Patent Application
20200197370
This patent application claims priority to Indian Provisional Patent Application number 2472/MUM/2014 (filed on Aug. 1, 2014) the contents of which are incorporated by reference herein.
The present invention relates to a nanoparticulate formulation comprising a microsomal prostaglandin E synthases-1 (“mPGES-1”) inhibitor. Particularly, the present invention relates to a nanoparticulate formulation comprising an mPGES-1 inhibitor and one or more surface stabilizers; a process for preparing such formulation; and its use in treating pain and inflammation in a subject.
Inflammation is one of the common causes of many disorders including asthma, inflammatory bowel disease, rheumatoid arthritis, osteoarthritis, rhinitis, conjunctivitis and dermatitis. Inflammation also leads to pain. One of the major problems associated with existing treatments of inflammatory conditions is inadequate efficacy and/or the prevalence of side effects.
The enzyme cyclooxygenase (COX) converts arachidonic acid to an unstable intermediate, prostaglandin H2 (PGH2), which is further converted to other prostaglandins, including PGE2, PGF2α, PGD2, prostacyclin and thromboxane A2. Among all prostaglandin metabolites, PGE2 is particularly known to be a strong pro-inflammatory mediator, and is also known to induce fever and pain. The conversion of PGH2 to PGE2 by prostaglandin E synthases (PGES) may, therefore, represent a pivotal step in the propagation of inflammatory stimuli. There are two microsomal prostaglandin E synthases (mPGES-1 and mPGES-2), and one cytosolic prostaglandin E synthase (cPGES). mPGES-1 is an inducible PGES after exposure to pro-inflammatory stimuli. mPGES-1 is induced in the periphery and CNS by inflammation, and represents therefore a target for acute and chronic inflammatory disorders. PGE2 is a major prostanoid, produced from arachidonic acid liberated by phospholipases (PLAs), which drives the inflammatory processes. Arachidonic acid is transformed by the action of prostaglandin H synthase (PGH synthase, cycloxygenase) into PGH2 which is a substrate for mPGES-1, the terminal enzyme transforming PGH2 to the pro-inflammatory PGE2.
Agents that are capable of inhibiting the action of mPGES-1, and thus reducing the formation of the specific arachidonic acid metabolite PGE2, are beneficial in the treatment of inflammation. Blocking the formation of PGE2 in animal models of inflammatory pain results in reduced inflammation, pain and fever response (Kojima et. al, The Journal of Immunology 2008, 180, 8361-6; Xu et. al., The Journal of Pharmacology and Experimental Therapeutics 2008, 326, 754-63).
International Publication Nos. WO 2006/063466, WO 2007/059610, WO 2010/034796, WO 2010/100249, WO 2012/055995, WO 2012/110860 and WO 2013/038308 disclose numerous heterocyclic compounds which are stated to be inhibitors of the microsomal prostaglandin E synthase-1 (mPGES-1) enzyme.
U.S. Pat. Nos. 5,145,684 and 7,998,507 and PCT Application Publication No. WO2003//049718 disclose nanoparticulate compositions.
There is a need for new, improved formulations of mPGES-1 inhibitors and methods of making and using such formulations.
The present invention relates to a nanoparticulate formulation comprising an mPGES-1 inhibitor, for example a poorly soluble mPGES-1 inhibitor such as the compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide or its pharmaceutically acceptable salt, solvates, hydrates or other derivative including esters and prodrug. The nanoparticulate formulation provides enhanced dissolution of the mPGES-1 inhibitor. Furthermore, the nanoparticles of the present invention are stable (e.g., with respect to particle size distribution, dissolution profile, and drug content over time) and provide a desirable dissolution profile.
In one embodiment, the nanoparticulate formulation comprises the compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (compound I) or its pharmaceutically acceptable salt and one or more surface stabilizers.
The nanoparticles preferably comprise the mPGES-1 inhibitor and one or more surface stabilizers.
In one of the embodiment nanoparticulate formulation comprises a compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (compound I) or its pharmaceutically acceptable salt and one or more surface stabilizer selected from the group consisting of polymers (also referred to as a polymer stabilizer or polymeric stabilizer) and surfactants. The compound I acts as an mPGES-1 inhibitor in the formulations and pharmaceutical compositions described herein.
In another embodiment, the nanoparticulate formulation comprises compound I or a pharmaceutically acceptable salt thereof wherein compound I or pharmaceutically acceptable salts thereof, has an effective average particle size in the range from about 20 nm to about 1000 nm. The formulation may comprise a therapeutically effective amount of compound I or its pharmaceutically acceptable salt, for example, an amount effective to inhibit mPGES-1 in a subject. The nanoparticulate formulation may further comprise one or more pharmaceutically acceptable excipients.
In an embodiment, the nanoparticulate particles may exist in a crystalline phase, an amorphous phase, a semi-crystalline phase, a semi amorphous phase, or a mixture thereof.
In one embodiment, the nanoparticulate formulation comprises from about 2% to about 15% by weight of an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof), such as from about 5 to about 10% by weight of an mPGES-1 inhibitor, based upon 100% total weight of the formulation.
In another embodiment, the nanoparticulate formulation comprises from about 15% to about 80% by weight of an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) based upon 100% total weight of the formulation.
A nanoparticulate formulation comprising a compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (compound I) or its pharmaceutically acceptable salt and one or more surface stabilizers selected from a group consisting of a polymer and a surfactant.
In one embodiment, the surface stabilizer may be a polymer selected from one or more from polyvinyl pyrrolidone, copovidone, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropylmethyl cellulose, polyethylene glycol, natural gums, cellulose derivatives and combinations thereof.
In another embodiment, the weight ratio of the mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) to the polymer stabilizer ranges from about 1:0.01 to about 1:100, or more preferable from about 1:0.1 to about 1:50.
In another embodiment, the nanoparticulate formulation comprises an mPGES1 inhibitor (such as compound 1 or its pharmaceutically acceptable salt) and one or more surface stabilizers wherein the surface stabilizer is a surfactant selected from poloxamer, polyoxyethylene sorbitan esters, polyethoxylated castor oil, glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, vitamin E TPGS, soya lecithin, or combinations thereof.
The nanoparticulate formulation may have a weight ratio of the mPGES-1 inhibitor (such as compound I or its pharmaceutically acceptable salt) to the surfactant ranging from about 1:0.01 to about 1:100 or from about 1:0.1 to about 1:50.
Another embodiment relates to nanoparticulate formulation comprising a compound I or its pharmaceutically acceptable salt, a polymer and a surfactant, wherein the formulation has an effective average particle size in the range from about 20 nm to about 1000 nm.
In another embodiment, the formulation has an effective average particle size in the range from about 50 nm to about 600 nm, more preferably from about 70 nm to 500 nm, more preferably from about 80 nm to 400 nm.
In one embodiment, the nanoparticles have a D10 value in the range from about 10 nm to about 300 nm, or preferably from about 20 nm to about 200 nm. In another embodiment, the nanoparticles have a D80 value in the range from about 100 nm to about 1000 nm, or preferably from about 200 nm to about 800 nm.
In yet another embodiment, the effective average particle size is in the range from about 70 nm to about 500 nm or from about 80 nm to about 400 nm. In one aspect of this embodiment, the D10 value is in the range from about 50 nm to about 200 nm. In another aspect the D80 value is in the range from about 300 nm to about 800 nm.
In one embodiment, the nanoparticulate formulation comprises an mPGES1 inhibitor (compound 1 or a pharmaceutically acceptable salt thereof) and one or more surface stabilizers wherein the surface stabilizer is selected from polymer such as polyvinyl pyrrolidone, copovidone, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropylmethyl cellulose, polyethylene glycol, natural gums, cellulose derivatives and combinations thereof. The weight ratio of the compound I or its pharmaceutically acceptable salt to the polymer may range from about 1:0.01 to 1:100 or about 1:0.1 to about 1:50.
In another embodiment, the said nanoparticulate formulation comprises an mPGES1 inhibitor (compound I or pharmaceutically acceptable salt thereof) and one or more surface stabilizers wherein the stabilizer is selected from surfactants such as poloxamer, polyoxyethylene sorbitan esters, polyethoxylated castor oil, glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, vitamin E TPGS, soya lecithin, and combinations thereof. The weight ratio of compound I or its pharmaceutically acceptable salt to the surfactant may range from about 1:0.01 to about 1:100 or from about 1:0.1 to about 1:50.
Yet another embodiment is a nanoparticulate formulation comprising i) an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof), ii) mannitol, iii) sodium lauryl sulphate, iv) hydroxy propyl methyl cellulose, v) poloxamer or vitamin ETPGS, wherein the formulation has an effective average particle size in the range from about 70 nm to about 500 nm, more preferably from 80 nm to 400 nm.
Another embodiment is a pharmaceutical composition comprising the nanoparticulate formulation described herein. The pharmaceutical formulation can be in the form of various dosage forms including, but not limited to, a dispersion, gel, aerosol, ointment, cream, lotion, paste, spray, film, patch, tablet, capsules, powder, granules, dry syrup, syrup or parenteral preparation such as preparation for intravenous, intra-arterial, intramuscular, intra-articular, or subcutaneous injection.
In a preferred embodiment, the pharmaceutical composition is present in the form of a dispersion, liquid solution, suspension, semi-solid preparation, granules, powders, tablet or capsules.
In one embodiment, the present invention relates to a pharmaceutical composition comprising a nanoparticulate formulation of the invention and one or more pharmaceutically acceptable excipients.
In an embodiment, the present invention also relates to a pharmaceutical composition comprising a nanoparticulate formulation comprising particles of an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof), one or more surface stabilizers and one or more pharmaceutically acceptable excipients. The nanoparticles have an effective average particle size in the range from about 20 nm to about 1000 nm.
The nanoparticulate formulation can be administered by an appropriate route which includes, but is not limited to, the oral, pulmonary, rectal, ophthalmic, parenteral, intravaginal, local, buccal, nasal or topical route. Preferably, the nanoparticulate formulation is suitable for oral administration.
In one embodiment, the pharmaceutical composition described herein is an immediate release composition suitable for oral administration.
In another embodiment, the pharmaceutical composition is an extended release or a delayed release composition suitable for oral administration.
Yet another embodiment is a process for the preparation of a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) and one or more surface stabilizers. The process may include (a) reducing the size of particles in an aqueous suspension, where the particles comprise an mPGES-1 inhibitor and one or more surface stabilizer (e.g., to an average particle size below 1000 nm), and (b) optionally spray drying the suspension. The particles in step (a) may be reduced by any method known in the art, including with a bead mill or high pressure wet milling. In one embodiment, the process comprises the steps of:
Yet another embodiment is a process for the preparation of a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) and one or more surface stabilizers. The process comprises the steps of:
Yet another embodiment is a process for preparation of a nanoparticulate formulation comprising the mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) and one or more surface stabilizer which is a mixture of a polymer (i.e., a polymeric stabilizer) and a surfactant. The process comprises the steps of:
The present invention also relates to a nanoparticle formulation for the treatment of an inflammation and/or pain in a subject, comprising compound I or its pharmaceutically acceptable salt and one or more surface stabilizer, wherein the formulation has an effective average particle size in the range from about 20 nm to about 1000 nm.
In one embodiment, the present invention relates a nanoparticle formulation for the treatment of an inflammation and/or pain or a disease or condition associated with pain and/or inflammation in a subject, comprising the compound I or a pharmaceutically acceptable salt thereof and a surface stabilizer; wherein the nanoparticles have an effective average particle size in the range from about 20 nm to 1000 nm, preferably from about 30 nm to about 800 nm, preferably from about 50nm to about 600nm, more preferably from about 70 nm to about 500 nm, more preferably from about 80 nm to about 400 nm.
In a further embodiment, the nanoparticulate formulation can be administered to the subject in need thereof once daily, twice daily, thrice daily or four times a day.
In yet another embodiment, the nanoparticulate formulation can be administered to a subject in need thereof at a dose range of about 10mg to about 500mg of compound I or its pharmaceutically acceptable salt.
The term “active ingredient” (used interchangeably with “active” or “active substance” or “drug”) as used herein refers to an mPGES -1 inhibitor. Preferably, the mPGES-1 inhibitor is N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (hereinafter, “compound I”) having structural formula:
or its pharmaceutically acceptable salt, solvate, hydrate or other derivatives including esters and prodrug.
By “salt” or “pharmaceutically acceptable salt”, it is meant those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, and allergic response, commensurate with reasonable benefit to risk ratio, and effective for their intended use. Representative acid additions salts include hydrochloride, hydrobromide, sulphate, bisulphate, acetate, oxalate, valerate, oleate, palmitate, stearate, laurate, borate, benzoate, lactate, phosphate, tosylate, mesylate, citrate, maleate, fumarate, succinate, tartrate, ascorbate, glucoheptonate, lactobionate, and lauryl sulphate salts. Representative alkali or alkaline earth metal salts include sodium, calcium, potassium and magnesium salts.
The term “surface stabilizer” as used herein includes agents which associate with the surface of particles of the mPGES-1 inhibitor, but do not chemically bond to or interact with it. Without being bound by any particular theory, it is believed that the surface stabilizer provides steric and/or ionic barriers to prevent agglomeration of the particles.
The term “nanoparticulate formulation” as used herein refers to a pharmaceutical dispersion wherein drug particles are dispersed in a solvent and have an effective average particle size of less than about 1000 nm.
As used herein, the term “average particle size” (or synonymously, “mean particle size”) refers to the distribution of particles, wherein about 50 volume percent of all the particles measured have a size less than the defined average particle size value. This can be identified by the term “D50” or “d (0.5)”.
As used herein, the term “D10” refers to the distribution of particles wherein about 10 volume percent of all the particles measured have a size less than the defined particle size value. This can be identified by the term “d (0.1)” as well. Similarly, as used herein, the term “D80” refers to the distribution of particles wherein about 80 volume percent of all the particles measured have a size less than the defined particle size value. This can be identified by the term “d (0.8)” as well. On similar lines, as used herein, the term “D90” refers to the distribution of particles wherein about 90 volume percent of all the particles measured have a size less than the defined particle size value. This can be identified by the term “d (0.9)” as well.
The particle size can be measured using various techniques such as laser diffraction, photon correlation spectroscopy (PCS) and Coulter's principle. When PCS is used as the method of determining particle size, the average particle size is the Z-average particle diameter known to those skilled in the art. Typically, instruments such as a ZETASIZER® 3000 HS (Malvern® Instruments Ltd., Malvern, United Kingdom), NICOMP 388™ ZLS system (PSS-Nicomp Particle Sizing Systems, Santa Barbara, Calif., USA), or Coulter Counter are used to determine the average particle size. Preferably, a Mastersizer 2000 (Malvern® Instruments Ltd., Malvern, United Kingdom) is used to determine the particle size of the particles.
By “an effective average particle size in the range from about 20 nm to about 1000 nm” it is meant that at least 50% of the total particles of compound I or its salt have a particle size in the range from about 20 nm to about 1000 nm when measured by the techniques mentioned herein. It is preferred that at least about 80% or at least about 90% of the particles have a particle size less than the effective average particle size, e.g., 1000 nm.
By “an effective average particle size in the range from about 30 nm to about 800 nm” it is meant that at least 50% of the total particles of compound I or its salt have a particle size in the range from about 30 nm to about 800 nm when measured by the techniques mentioned herein.
By “an effective average particle size in the range from about 50 nm to about 600 nm” it is meant that at least 50% of the total particles of compound I or its salt have a particle size in the range from about 50 nm to about 600 nm when measured by the techniques mentioned herein.
By “an effective average particle size in the range from about 70 nm to about 500 nm” it is meant that at least 50% of the total particles of compound I or its salt have a particle size in the range from about 70 nm to about 500 nm when measured by the techniques mentioned herein.
By “an effective average particle size in the range from about 80 nm to about 400 nm” it is meant that at least 50% of the total particles of compound I or its salt have a particle size in the range from about 80 nm to about 400 nm when measured by the techniques mentioned herein.
By “pharmaceutically acceptable excipient” it is meant any of the components of a formulation or pharmaceutical composition other than the active ingredient, and which are approved by regulatory authorities or are generally regarded as safe for human or animal use.
The term “treating” or “treatment” as used herein includes the prophylaxis, mitigation, prevention, amelioration, or suppression of a disorder modulated by the mPGES -1 inhibitor in a subject.
The term “effective amount” or “therapeutically effective amount” when used in conjunction with an mPGES-1 inhibitor denotes an amount of an active ingredient that, when administered to a subject for treating a state, disorder or condition, produces an intended therapeutic benefit in a subject.
The term “subject” includes mammals such as humans and other animals, such as domestic animals (e.g., household pets including cats and dogs) and non-domestic animals (such as wildlife). Preferably, the subject is a human.
“Pain” a complex constellation of unpleasant sensory, emotional and cognitive experiences provoked by real or perceived tissue damage and manifested by certain autonomic, psychological and behavioral reactions and is a disease of epidemic proportions. From a neurobiological perspective, pain is believed to be of three different aspects: first, pain that is an early warning physiological protective system, essential to detect and minimize contact with damaging or noxious stimuli and is called ‘nociceptive pain’; second, pain is adaptive and protective, by heightening sensory sensitivity after unavoidable tissue damage, which is mainly caused by activation of the immune system by tissue injury or infection and is normally called ‘inflammatory pain’; and the third type of pain which is not protective, but maladaptive resulting from abnormal functioning of the nervous system and generally called as ‘pathological pain’. This pathological pain is not a symptom of some disorder but rather a disease state of the nervous system and can occur after damage to the nervous system (neuropathic pain) or a situation where there is no such damage or inflammation (dysfunctional pain—like fibromyalgia, irritable bowel syndrome, temporomandibular joint disease, interstitial cystitis and other syndromes where there is substantial pain but no noxious stimulants and minimal/no peripheral inflammatory pathology).
Pain can also have different qualities and temporal features depending on the modality and locality of the stimulus, respectively—firstly pain can be described as lancinating, stabbing or pricking; and secondly more pervasive including burning, throbbing, cramping, aching and sickening. It is believed to be one of the leading causes of joint movement limitations and disability.
The mPGES-1 Inhibitor
Suitable mPGES-1 inhibitors include, but are not limited to, those disclosed in co-assigned International Publication No. WO 2013/186692 (“the '692 application”), which is hereby incorporated by reference in its entirety. These mPGES-1 inhibitors are useful for the treatment of pain and inflammation in a variety of diseases and conditions. One preferred mPGES-1 inhibitor disclosed in the '692 application is N-(4-chloro-3-(5-oxo-1-(4-(trifuloromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (hereinafter, “compound I”) having the structural formula:
or its pharmaceutically acceptable salt, solvates and hydrates and other derivatives including esters and prodrugs.
The surface stabilizer may be one or more polymers, one or more surfactants, or a combination thereof. Suitable polymers include, but are not limited to, cellulose derivatives, such as hydroxypropyl methyl cellulose(hypromellose), hydroxypropyl cellulose, methyl cellulose, carboxymethyl cellulose sodium or calcium salt, hydroxyl ethyl cellulose, polyvinyl pyrrolidone, copovidone, carbopols, copolymers of polyvinyl pyrrolidone, polyoxyethylene alkyl ether, polyethylene glycol, co-block polymers of ethylene oxide and propylene oxide (poloxamer®, Pluronic®), poly methacrylate derivatives, polyvinyl alcohol, polyvinyl alcohol derivatives and polyethylene glycol derivatives, such as macrogol glycerol stearate, natural gums such as xanthan gum, locust bean gum, alginic acid, carrageenan, and sodium alginate. Preferred polymers include, but are not limited to, polyvinyl pyrrolidone, copovidone, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropylmethyl cellulose, polyethylene glycol, magnesium aluminum silicate, cellulose derivatives and natural gums.
Suitable surfactants include, but are not limited to, poloxamer, polyoxyethylene sorbitan esters (such as polysorbate or Tween® available from Sigma-Aldrich of St. Louis, Mo.), polyethoxylated castor oil (such as Cremophor® available from BASF of Florham Park, N.J.), methyl glucose sesquistearate, PEG-20 methyl glucoside sesquistearate, caprylocaproyl macrogol-8 glycerides, lauroyl macrogol-32-glycerides, Steareth-21, soluplus, polyethylene glycol 20 sorbitan monostearate, polyethylene glycol 60 sorbitan monostearate, polyethylene glycol 80 sorbitan monostearate, Steareth-20, Ceteth-20, PEG-100 stearate, sodium stearoyl sarcosinate, hydrogenated lecithin, sodium cocoylglyceryl sulfate, sodium stearyl sulfate, sodium stearoyl lactylate, PEG-20 glyceryl monostearate, sucrose monostearate, sucrose polystearates, polyglyceryl 10 stearate, polyglyceryl 10 myristate, steareth 10, DEA oleth 3 phosphate, DEA oleth 10 phosphate, PPG-5 Ceteth 10 phosphate sodium salt, PPG-5 Ceteth 10 phosphate potassium salt, steareth-2, PEG-5 soya sterol oil, PEG-10 soya sterol oil, diethanolamine cetyl phosphate, sorbitan monostearate, diethylenglycol monostearate, glyceryl monostearate, sodium stearyl sulfate, benzalkonium chloride, docusate sodium, triethanolamine, and phospholipids. Preferred surfactants include, but are not limited to, polyoxyethylene sorbitan esters (such as polysorbate or Tween®), polyethoxylated castor oil (such as cremophor®), glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, Vitamin E TPGS, and soya lecithin. In one embodiment, the surfactant is selected from poloxamer, polyoxyethylene sorbitan esters (such as polysorb ate or Tween®), polyethoxylated castor oil (such as Cremophor®), glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, vitamin E TPGS, and soya lecithin.
One embodiment is a nanoparticulate formulation comprising an mPGES-1 inhibitor, such as the compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (compound I) or its pharmaceutically acceptable salt and one or more surface stabilizers.
In an embodiment, the said nanoparticulate formulation may further comprise one or more pharmaceutically acceptable excipients.
The present invention relates to a nanoparticulate formulation comprising a compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl)benzyl)pivalamide (compound 1) or its pharmaceutically acceptable salt and one or more surface stabilizers selected from the group consisting of polymers or surfactants.
In another embodiment, the nanoparticulate formulation comprising the compound I or a pharmaceutically acceptable salt thereof wherein compound I having an effective average particle size in the range from about 20 nm to about 1000 nm. The nanoparticulate formulation may further comprise one or more pharmaceutically acceptable excipients.
In one embodiment, the nanoparticulate formulation has an effective average particle size in the range from about 30 nm to about 800 nm, preferably from about 50 nm to about 600 nm, more preferably from about 80 nm to about 400 nm.
In one embodiment, the nanoparticulate formulation comprise from about 2 to about 15% by weight of an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof), such as from about 5 to about 10% by weight of an mPGES-1 inhibitor, based upon 100% total weight of the formulation.
In another embodiment, the said nanoparticulate formulation comprise from about 15 to about 80% by weight of an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) based upon 100% total weight of the formulation.
In a preferred embodiment, the present invention provides a nanoparticulate formulation comprising compound I or a pharmaceutically salt thereof and a surface stabilizer selected from a polymer, a surfactant, and/or combination thereof.
The formulation may have an effective average particle size in the range from about 30 nm to about 800 nm, or preferably from about 50 nm to about 600 nm, more preferably from about 80 nm to about 400 nm.
Another embodiment is a nanoparticulate formulation comprising particles of compound I or a pharmaceutically salt thereof and a surface stabilizer selected from polyvinyl pyrrolidone, copovidone, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropylmethyl cellulose, polyethylene glycol, natural gums, cellulose derivatives and combinations thereof., where the particles have an effective average particle size in the range from about 20 nm to about 1000 nm, from about 30 nm to about 800 nm, or from about 50 nm to about 600 nm.
In one embodiment, surface stabilizer comprises copovidone, poloxamer, sodium lauryl sulfate, and polyethylene glycol, and any combination of any of the foregoing. The particles may also include a diluent, such as mannitol. In one preferred embodiment, the particles have an effective average particle size in the range from about 70 nm to about 500 nm or from about 80 nm to about 400 nm.
In one embodiment, the surface stabilizer is selected from one or more polymers selected from polyvinyl pyrrolidone, copovidone, hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropylmethyl cellulose, polyethylene glycol, natural gums, cellulose derivatives and combinations thereof.
In another embodiment, the weight ratio of the mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof) to the polymer stabilizer ranges from about 1:0.01 to about 1:100, or more preferable from about 1:0.1 to about 1:50.
In another embodiment, the nanoparticulate formulation comprises an mPGES1 inhibitor (such as compound I or its pharmaceutically acceptable salt) and a surface stabilizer which is a surfactant selected from poloxamer, polyoxyethylene sorbitan esters, polyethoxylated castor oil, glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, vitamin E TPGS, soya lecithin, or combinations thereof.
In further embodiment, the nanoparticulate formulation may have a weight ratio of the mPGES-1 inhibitor (such as compound I or its pharmaceutically acceptable salt) to the surfactant ranging from about 1:0.01 to about 1:100 or from about 1:0.1 to about 1:50.
In another embodiment, the nanoparticles have a D10 value in the range from about 1 nm to about 500 nm, or preferably from about 5 nm to about 200 nm. In another aspect of this embodiment, the nanoparticles have a D80 value in the range from about 100 nm to about 1000 nm, or preferably from about 200 nm to about 800 nm.
In yet another embodiment, the effective average particle size of the nanoparticles is in the range from about 70 nm to about 500 nm or from about 80 nm to about 400 nm. In one aspect of this embodiment, the D10 value is in the range from about 5 nm to about 200 nm. In another aspect the D80 value is in the range from about 300 nm to about 800 nm.
All combinations of the particle size ranges are contemplated to be within the scope of this invention. For example, the nanoparticles may have a D10 value of from about 1 nm to about 500 nm as well as a D80 value of from about 200 to about 800 nm.
In another embodiment, the surfactant is selected from poloxamer, polyoxyethylene sorbitan esters, polyethoxylated castor oil, glycerol monostearate, phospholipids, benzalkonium chloride, triethanolamine, sodium lauryl sulfate, docusate sodium, Vitamin E TPGS, soya lecithin, and any combination thereof.
In another embodiment, the present invention relates to a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as Compound I or a pharmaceutically acceptable salt thereof), mannitol, sodium lauryl sulphate, Hydroxy propyl methyl cellulose, poloxamer or vitamin ETPGS.
Yet another embodiment is a nanoparticulate formulation comprising i) an mPGES-1 inhibitor (such as compound I or a pharmaceutically acceptable salt thereof), ii) mannitol, iii) sodium lauryl sulphate, iv) hydroxy propyl methyl cellulose, and poloxamer or vitamin ETPGS, wherein the formulation has an effective average particle size in the range from about 70 nm to about 500 nm, more preferably from 80 nm to 400 nm.
The nanoparticles may further include one or more pharmaceutically acceptable excipients, such as a diluent. Non-limiting examples of diluents include one or more of microcrystalline cellulose, silicified microcrystalline cellulose (e.g., Prosolv®), microfine cellulose, lactose, starch, pregelatinized starch, mannitol, sorbitol, dextrates, dextrin, maltodextrin, dextrose, calcium carbonate, calcium sulfate, dibasic calcium phosphate dihydrate, tribasic calcium phosphate, magnesium carbonate, magnesium oxide, and combinations thereof. Other examples of diluents include (1) cores or beads comprising insoluble inert materials such as glass particles/beads or silicon dioxide, calcium phosphate dihydrate, dicalcium phosphate, calcium sulfate dihydrate, or cellulose derivatives; (2) soluble cores such as sugar spheres of sugars such as dextrose, mannitol, sorbitol, or sucrose; (3) insoluble inert plastic materials such as spherical or nearly spherical core beads of polyvinyl chloride, polystyrene or any other pharmaceutically acceptable insoluble synthetic polymeric material, 4) acacia, guar gum, alginic acid, dextrin, maltodextrin, methylcellulose, ethyl cellulose, hydroxyethyl cellulose, hydroxypropyl cellulose (e.g., Klucel®), low substituted hydroxypropyl cellulose, hydroxypropyl methylcellulose (e.g., Methocel®), carboxymethyl cellulose sodium, povidone (various grades of Kollidon®, Plasdone®), carboxymethyl cellulose calcium, croscarmellose sodium, (e.g., Ac-Di-Sol®, Primellose®), crospovidone (e.g., Kollidon®, Polyplasdone®), povidone K-30, polacrilin potassium, sodium starch glycolate (e.g., Primogel, Explotab®), and combinations thereof.
The present invention relates to the nanoparticulate formulation which can be administered by an appropriate route which includes, but is not limited to, the oral, pulmonary, rectal, ophthalmic, parenteral, intravaginal, local, buccal, nasal or topical route. Preferably, the nanoparticulate formulation is suitable for oral administration.
The nanoparticulate formulation can be converted or incorporated into a suitable pharmaceutical composition which includes, but is not limited to, dispersion, gel, aerosol, ointment, cream, lotion, paste, spray, film, patch, tablets, capsules, powder, granules, dry syrup, syrup and parenteral preparations such as intravenous, intra-arterial, intramuscular, intra-articular, and subcutaneous injections.
In a preferred embodiment, the nanoparticulate formulation is in the form of a dispersion, liquid suspension, semi-solid suspension, powder, granules, tablets or capsules.
In one embodiment, the pharmaceutical composition is an immediate release composition suitable for oral administration.
In another embodiment, the pharmaceutical composition is an extended release or a delayed release composition suitable for oral administration. The nanoparticulate formulation of the present invention can be administered as such, or alternately, it can be further converted into a suitable pharmaceutical composition such as solid, liquid or semi-solid preparation for ease of administration. The pharmaceutical composition may be prepared by conventional methods known in the art.
In one embodiment, the present invention relates to a pharmaceutical composition comprising the nanoparticulate formulation of the invention and one or more pharmaceutically acceptable excipients.
Suitable pharmaceutically acceptable excipients include, but are not limited to one or more of diluents, glidants and lubricants, preservatives, buffering agents, chelating agents, polymers, opacifiers, colorants, gelling agents and viscosifying agents, antioxidants, disintegrants, solvents, co-solvents, and combinations thereof.
Non-limiting examples of glidants and lubricants include one or more of stearic acid, magnesium stearate, talc, colloidal silicon dioxide, and sodium stearyl fumarate.
Non-limiting examples of preservatives include one or more of phenoxyethanol, parabens such as methyl paraben and propyl paraben and their sodium salts, propylene glycols, sorbates, urea derivatives such as diazolindinyl urea, and mixtures thereof. Non-limiting examples of buffering agents include sodium hydroxide, potassium hydroxide, ammonium hydroxide and mixtures thereof. Non-limiting examples of chelating agents include ethylene diamine tetraacetic acid (“EDTA”), and disodium edetate and EDTA derivatives.
Non-limiting examples of polymers include one or more of gum arabic, sodium based lignosulfonate, methyl methacrylate, methacrylate copolymers, isobutyl methacrylate, and ethylene glycol dimethacrylate.
Non-limiting examples of gelling agents and viscosifying agents include one or more of carbomers (carbopol), modified cellulose derivatives, naturally-occurring, synthetic or semi-synthetic gums such as xanthan gum, acacia and tragacanth, sodium alginate, gelatin, modified starches, cellulosic polymers such as hydroxypropyl cellulose, hydroxyethyl cellulose, hydroxypropyl methylcellulose, hydroxypropyl methylcellulose phthalate, and methyl cellulose, co-polymers such as those formed between maleic anhydride and methyl vinyl ether, colloidal silica, methacrylate derivatives, polyethylene oxides, polyoxyethylene-polyoxypropylene copolymers, and polyvinyl alcohol.
Non-limiting examples of co-solvents include one or more of propylene glycol, polyol esters of fatty acids, trialkyl citrate esters, propylene carbonate, dimethylisosorbide, ethyl lactate, N-methylpyrrolidones, transcutol, glycofurol, decaglycerol mono-, dioleate (Caprol PGE-860), triglycerol monooleate (Caprol 3GO), polyglycerol oleate (Caprol MPGO), mixed diesters of Caprylic/Capric acid and propylene glycol (Captex 200), glyceryl mono- and di-caprate (Capmul MCM), isostearyl isostearate, oleic acid, peppermint oil, oleic acid, soybean oil, safflower oil, corn oil, olive oil, cottonseed oil, arachis oil, sunflower seed oil, palm oil, rapeseed oil, ethyl oleate, glyceryl monooleate, and vitamin E TPGS.
Non-limiting examples of solvents include one or more of water; tetrahydrofuran; propylene glycol; liquid petrolatum; ether; petroleum ether; alcohols, e.g., methanol, ethanol, isopropyl alcohol and higher alcohols; alkanes, e.g., pentane, hexane and heptane; ketones, e.g., acetone and methyl ethyl ketone; chlorinated hydrocarbons, e.g., chloroform, carbon tetrachloride, methylene chloride and ethylene dichloride; acetates, e.g., ethyl acetate; lipids, e.g., isopropyl myristate, diisopropyl adipate and mineral oil.
The nanoparticulate formulations and pharmaceutical compositions are stable (e.g., with respect to particle size distribution, dissolution profile, and drug content over time) and provide a desirable dissolution profile. For example, in one embodiment, the nanoparticulate formulation or pharmaceutical composition exhibits less than a 4, 5, or 10% variation in the amount of drug dissolved in 60 minutes when tested initially and after 3 or 6 months of storage under standard conditions (25° C. and 60% relative humidity) or accelerated conditions (40° C. and 75% relative humidity).
In another embodiment, the nanoparticulate formulation or pharmaceutical composition exhibits less than 0.5, 1, or 2% total impurities when tested initially and after 3 or 6 months of storage under standard conditions (25° C. and 60% relative humidity) or accelerated conditions (40° C. and 75% relative humidity). In yet another embodiment, the nanoparticulate formulation or pharmaceutical composition exhibits less than a 3, 5, or 7% variation in the drug content when tested initially and after 3 or 6 months of storage under standard conditions (25° C. and 60% relative humidity) or accelerated conditions (40° C. and 75% relative humidity).
In an embodiment, the nanoparticulate formulation is in the form of granules that are rapidly dissolvable, for example, dissolving at least 80% of the drug content within 60 minutes, when measured using a USP type II (paddle) apparatus in 900 mL of 0.1 N HCl and 3% to 5% cetyl trimethyl ammonium bromide (CTAB) at 37±0.5° C. and a speed of 100 rpm.
In another embodiment, the nanoparticulate formulations are rapidly dissolvable, for example, dissolving at least 80% of the drug content within 60 minutes can also be tested using a USP type II (paddle) apparatus in 900 mL of 0.1 N HCl at 37±0.5° C. and a speed of 50 rpm.
The preparation of the nanoparticulate formulation (or pharmaceutical composition containing the nanoparticulate formulation) may include various unit operations such as milling, micronization, mixing, homogenizing, sifting, spraying, solubilizing, dispersing, granulating, lubricating, compressing, coating, and/or filling. These processes may be used for preparing the nanoparticulate formulation and pharmaceutical composition of the present invention. The reduction of the particle size may be achieved using various techniques such as dry or wet milling, micronization, high pressure homogenization, controlled precipitation using an anti-solvent, microfluidization and/or supercritical fluid technology.
One embodiment relates to a process for preparation of a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as compound I or its pharmaceutically acceptable salt) and a surface stabilizer. The process comprises the steps of:
Yet another embodiment is a process for preparation of a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as compound I or its pharmaceutically acceptable salt) and one or more surface stabilizers. The process comprises the steps of:
Yet another embodiment is a process for preparation of a nanoparticulate formulation comprising an mPGES-1 inhibitor (such as Compound I or its pharmaceutically acceptable salt) and one or more surface stabilizer. The process comprises the steps of:
The present invention also relates to a method of treating pain and/or inflammation or a disease or condition associated with pain and/or inflammation (for example, such a disease or condition which is mediated by mPGES-1) by administering to a subject the nanoparticulate formulation (or pharmaceutical composition containing the nanoparticulate formulation) as described herein.
The present invention also relates to a nanoparticle formulation for the treatment of an inflammation and/or pain in a subject, comprising the compound N-(4-chloro-3-(5-oxo-1-(4-(trifluoromethyl)phenyl)-4,5-dihydro-1H-1,2,4-triazol-3-yl) benzyl) pival amide (“compound I”) or its pharmaceutically acceptable salt and a surface stabilizer, wherein the formulation has an effective average particle size in the range from about 20 nm to about 1000 nm.
In one embodiment, the present invention relates to a nanoparticulate formulation for treating pain and/or inflammation or a disease or condition associated with pain and/or inflammation, comprising an mPGES-1 inhibitor (such as compound I or its pharmaceutically acceptable salt) and a surface stabilizer; where the formulation has an effective average particle size in the range from about 20 nm to about 1000 nm. In one embodiment, the effective average particle size is in the range from about 30 nm to about 800 nm, from about 50 nm to 600 nm, from about 70 nm to about 500 nm, or from about 80 nm to 400 nm.
In further embodiment, the said nanoparticulate formulation can be administered to the subject in need thereof once daily, twice daily, thrice daily or four times a day.
In yet another embodiment, the nanoparticulate formulation comprising compound-1 as mPGES-1 inhibitor can be administered to the subject in need thereof at a dose of the mPGES-1 inhibitor of about 10 mg to about 500 mg.
It will be understood that various modifications may be made to the embodiments disclosed herein. Therefore the above description should not be construed as limiting, but merely as exemplifications of preferred embodiments. Other arrangements and methods may be implemented by those skilled in the art without departing from the scope and spirit of this invention.
The following examples are provided to enable one skilled in the art to practice the invention and are merely illustrative of the invention. The examples should not be read as limiting the scope of the invention.
Manufacturing process:
The particle size of the compound I was determined in water using a Mastersizer 2000 (Malvern Instruments Ltd., Malvern, United Kingdom). Three readings were taken for each measurement, and the average size was reported.
Manufacturing process:
The pharmaceutical composition was subjected to accelerated stability studies at a temperature of 40° C.±2° C. and a relative humidity of 75%±5% for a period of 3 months. The drug assay was performed and active contents were analyzed using HPLC. In-vitro dissolution studies were performed using USP type II (Paddle) apparatus in 900 ml 0.1N HCl and 3% cetyl trimethyl ammonium bromide (CTAB) as the Dissolution Medium at a temperature of 37±0.5 ° C. and a speed of 100 revolutions per minute (RPM) for a period of 60 minutes. The aliquots taken out at 60 minutes were analyzed for active content by HPLC technique. The HPLC parameters include Inertsil ODS 3V, 150×4.6 mm, 5 μm column at a flow rate of 1.0 ml/min, detection wavelength of 270 nm, column temperature of 25° C., injection volume of 20μ1 and run time of 14 minutes. Aqueous orthophosphoric acid buffer (pH 2.5): Acetonitrile in the ratio of 35:65 v/v was used as a mobile phase.
Stability and dissolution data for Example 2:
Manufacturing process:
The particle size data for the granules used in the capsules of Example 3 is provided below.
The compositions described above were prepared according to the process described in Example 1.
The compositions were prepared according to the process described in Example 1.
The compositions were prepared according to the process described in Example 6.
The tablet containing the nanoparticulate formulation is crushed to get a powder mass. The powder mass can be further subjected to Hot-stage Optical Microscopy technique as described in Yin et al., Journal of Pharmaceutical Sciences Vol. 94 No. 7, July 2005. Briefly, the powder mass is mounted on the slide, which is heated at a controlled rate (e.g., 10° C/min). The particles remaining at higher temperature are confirmed by DSC and variable-temperature powder X-ray diffraction to be crystalline drug particles.
Alternatively, the particle size of the nanoparticulate formulation in a tablet can also be determined by dispersing the tablet in a suitable solvent in which the excipients are highly soluble as against the nanoparticulate formulation such that the nanoparticulate formulation remains in dispersed form. Further, the particle size of the dispersion can be determined by the methods as described above.
In another method, particle size of Compound I containing granules in the pharmaceutical composition can be determined using a PXRD peak broadening technique, followed by applying the Scherrer equation T=Kλ/βτ cos θ where τ is the mean particle dimension, K is a constant of 0.9, λ is the X-ray wavelength, and βτ is the peak broadening value due to crystal size reduction, i.e., the full-width-at-half-maximal (FWHM) difference in radian at a certain Bragg angle (θ), between a nanoparticulate dispersion and the micronized excipients. (Dantuluri A. et. al., Sciforum e-conference ECPS 2011 Communication).
Further, different imaging techniques or methodologies can be used to expose the particulate formulation contained in the pharmaceutical compositions (e.g., tablet), wherein in situ particle size measurements can be performed. Several methods exist which are able to determine the particle size in a matrix, such as Raman spectroscopy, Transmission Electron Microscopy (TEM), Time of Flight Secondary Ion Mass Spectroscopy (TOF-SIMS), FTIR and NIR microscopy and micro-thermal analysis (μTA).
Although the invention herein has been described with reference to particular embodiments, it is to be understood that these embodiments are merely illustrative of the principles and application of the present invention. It is therefore to be understood that numerous modifications may be made to the illustrative embodiments.
All publications, patents, and patent applications cited in this application are herein incorporated by reference to the same extent as if each individual publication, patent, or patent application was specifically and individually indicated to be incorporated herein by reference.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2472/MUM/2014 | Aug 2014 | IN | national |
| Number | Date | Country | |
|---|---|---|---|
| Parent | 15318571 | Dec 2016 | US |
| Child | 16527817 | US |
