Nanostructured Magnetic Disks for Wide Range Size Selection in NGS Library Preparation and PCR Purification

Information

  • Research Project
  • 10212466
  • ApplicationId
    10212466
  • Core Project Number
    R44GM134994
  • Full Project Number
    3R44GM134994-03S1
  • Serial Number
    134994
  • FOA Number
    PA-18-591
  • Sub Project Id
  • Project Start Date
    8/18/2017 - 7 years ago
  • Project End Date
    3/31/2021 - 3 years ago
  • Program Officer Name
    KREPKIY, DMITRIY
  • Budget Start Date
    4/1/2020 - 4 years ago
  • Budget End Date
    3/31/2021 - 3 years ago
  • Fiscal Year
    2020
  • Support Year
    03
  • Suffix
    S1
  • Award Notice Date
    9/15/2020 - 4 years ago
Organizations

Nanostructured Magnetic Disks for Wide Range Size Selection in NGS Library Preparation and PCR Purification

Project Summary We proposed to develop Nanobind reaction purification and size selection methods for long-read sequencing library preparation. These methods use our Nanobind magnetic disks to purify enzymatic reactions and to enhance read lengths by removing short DNA during long-read sequencing library preparation. Nanobind disks are small 1-5 mm diameter magnetic disks covered with a high density of micro- and nanostructured silica. Compared to magnetic particles, Nanobind disks are extraordinarily gentle, preventing DNA shearing and enabling high efficiency recovery of DNA surpassing megabase in length. We currently sell DNA extraction kits that enable isolation of ultra high molecular weight (UHMW) megabase-sized DNA from a wide variety of sample types. To evaluate how gentle the Nanobind reaction purifications are, we tested them in an ultra-long sequencing protocol capable of generating 100 kb ? 1+ Mb length reads. This method is capable of generating extremely high read length N50 in the 100 ? 200 kb range and 10 times more megabase reads than previous ultra long methods. This supplement will allow additional development and sequencing tests on the Nanobind ultra long sequencing protocols. Successful validation of the method on PromethION will lead to a significant enhancement in data throughput and corresponding in reduction of cost, time, and manpower, enabling an immediate impact via translation into projects such as the NHGRI pan-genome project and a long-term impact by dramatically reducing the cost of ultra long sequencing.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R44
  • Administering IC
    GM
  • Application Type
    3
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    410712
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    172
  • Ed Inst. Type
  • Funding ICs
    NHGRI:410712\
  • Funding Mechanism
    SBIR-STTR RPGs
  • Study Section
  • Study Section Name
  • Organization Name
    CIRCULOMICS, INC.
  • Organization Department
  • Organization DUNS
    830377581
  • Organization City
    Baltimore
  • Organization State
    MD
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    212023183
  • Organization District
    UNITED STATES