Claims
- 1. An isolated nucleic acid molecule comprising a nucleotide sequence selected from the group consisting of:
a) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; b) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4; c) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 2; d) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 4; e) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 1; f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 3; g) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO: 1, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO: 1; h) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO: 3, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO: 3; i) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; j) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; and k) a nucleotide sequence complementary to at least one of the nucleotide sequences set forth in a), b), c), d), e), f), g), h), i), or j).
- 2. The isolated nucleic acid molecule of claim 1, wherein said nucleic acid molecule comprises a nucleotide sequence selected from the group consisting of:
a) the nucleotide sequence set forth in SEQ ID NO: 2; b) the nucleotide sequence set forth in SEQ ID NO: 4; c) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 1; d) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 3; e) a nucleotide sequence complementary to a nucleotide sequence of a), b), c), or d).
- 3. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule further comprises vector nucleic acid sequences.
- 4. The nucleic acid molecule of claim 1, wherein said nucleic acid molecule further comprises nucleic acid sequences encoding a heterologous polypeptide.
- 5. A host cell containing the nucleic acid molecule of claim 1.
- 6. The host cell of claim 5, wherein said host cell is a mammalian host cell.
- 7. A nonhuman mammalian host cell containing the nucleic acid molecule of claim 1.
- 8. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of:
a) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 1, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 1; b) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 3, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 3; c) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; d) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; e) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; and f) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4.
- 9. The isolated polypeptide of claim 8, wherein said polypeptide comprises an amino acid sequence selected from the group consisting of:
a) the amino acid sequence set forth in SEQ ID NO: 1; and b) the amino acid sequence set forth in SEQ ID NO: 3.
- 10. The polypeptide of claim 8, wherein said polypeptide further comprises heterologous amino acid sequences.
- 11. An antibody which selectively binds to a polypeptide of claim 8.
- 12. A method for producing a polypeptide selected from the group consisting of:
a) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 1, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 1; b) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 3, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 3; c) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; d) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; e) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; and f) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4; wherein said method comprises culturing a host cell comprising a nucleotide sequence encoding said polypeptide under conditions in which the polypeptide is expressed.
- 13. The method of claim 12, wherein said polypeptide comprises an amino acid sequence selected from the group consisting of:
a) the amino acid sequence set forth in SEQ ID NO: 1; and b) the amino acid sequence set forth in SEQ ID NO: 3.
- 14. A method for detecting the presence of a polypeptide in a sample comprising the steps of contacting the sample with a compound that selectively binds to the polypeptide and determining whether the compound binds to the polypeptide in the sample, wherein said polypeptide is selected from the group consisting of:
a) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 1, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 1; b) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 3, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 3; c) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; d) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; e) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; and f) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4.
- 15. The method of claim 14, wherein the compound which binds to the polypeptide is an antibody.
- 16. A kit comprising a compound which selectively binds to a polypeptide and instructions for use, wherein said polypeptide is selected from the group consisting of:
a) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 1, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 1; b) the amino acid sequence of a fragment of the amino acid sequence set forth in SEQ ID NO: 3, wherein the fragment comprises at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO: 3; c) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; d) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleic acid molecule that hybridizes to the complement of the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; e) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; and f) the amino acid sequence of a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said variant is encoded by a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4
- 17. A method for detecting the presence of a nucleic acid molecule in a sample, the method comprising the steps of contacting the sample with a nucleic acid probe that hybridizes to the nucleic acid molecule under stringent conditions, and determining whether the nucleic acid probe binds to a nucleic acid molecule in the sample, wherein said nucleic acid molecule is selected from the group consisting of:
a) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 2; b) a nucleotide sequence having at least 70% sequence identity with the nucleotide sequence set forth in SEQ ID NO: 4; c) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 2; d) a nucleotide sequence consisting of at least 20 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 4; e) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 1; f) a nucleotide sequence encoding the amino acid sequence set forth in SEQ ID NO: 3; g) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO: 1, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO: 1; h) a nucleotide sequence encoding a fragment of the amino acid set forth in SEQ ID NO: 3, wherein said fragment consists of at least 15 contiguous amino acids of SEQ ID NO: 3; i) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 1, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO: 2 under stringent conditions; j) a nucleotide sequence encoding a variant of the amino acid sequence set forth in SEQ ID NO: 3, wherein said nucleotide sequence hybridizes to the nucleotide sequence set forth in SEQ ID NO: 4 under stringent conditions; and k) a nucleotide sequence complementary to at least one of the nucleotide sequences set forth in a), b), c), d), e), f), g), h), i), or j).
- 18. The method of claim 17, wherein the sample comprises mRNA molecules.
- 19. A kit for use in the method of claim 17, wherein said kit comprises a compound which selectively hybridizes to at least one nucleic acid molecule of claim 1 and instructions for use.
- 20. A method for identifying a compound which binds to a polypeptide of claim 8, said method comprising the steps of:
a) contacting a polypeptide, or a cell expressing a polypeptide of claim 8 with a test compound; and b) determining whether the polypeptide binds to the test compound.
- 21. The method of claim 20, wherein the binding of the test compound to the polypeptide is detected by a method selected from the group consisting of:
a) detection of binding by direct detecting of test compound/polypeptide binding; b) detection of binding using a competition binding assay; c) detection of binding using an assay for programmed cell death protein-like-mediated activity.
- 22. A method for modulating the activity of a polypeptide of claim 8 comprising contacting a polypeptide or a cell expressing a polypeptide of claim 8 with a compound which binds to the polypeptide in a sufficient concentration to modulate the activity of the polypeptide.
- 23. A method for identifying a compound which modulates the activity of a polypeptide of claim 8, comprising:
a) contacting a polypeptide of claim 8 with a test compound; and b) determining the effect of the test compound on the activity of the polypeptide to thereby identify a compound which modulates the activity of the polypeptide.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. Provisional Application No. 60/262,306, filed Jan. 16, 2001.
Provisional Applications (1)
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Number |
Date |
Country |
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60262306 |
Jan 2001 |
US |