Claims
- 1. A nematode neuromuscular juntion GABA receptor complex of the formula I
- 2. A homopentamer nematode neuromuscular juntion GABA receptor complex of the formula II
- 3. A nematode neuromuscular junction GABA receptor complex subunit, comprising an amino acid sequence selected from the group consisting of:
(a) an amino acid sequence which is encoded by a nucleic acid sequence which has at least 80% identity to SEQ ID NO 2; wherein said identity can be determined using the DNAsis computer program and default parameters; and (b) an amino acid sequence which has at least 80% identity to SEQ ID NO 1; wherein said identity can be determined using the DNAsis computer program and default parameters.
- 4. A nematode neuromuscular junction GABA receptor complex subunit, comprising an amino acid sequence selected from the group consisting of:
(a) an amino acid sequence which is encoded by a nucleic acid sequence which has at least 80% identity to SEQ ID NO 4; wherein said identity can be determined using the DNAsis computer program and default parameters; and (b) an amino acid sequence which has at least 80% identity to SEQ ID NO 3; wherein said identity can be determined using the DNAsis computer program and default parameters.
- 5. A nucleic acid compound which encodes a nematode neuromuscular junction GABA receptor complex subunit, comprising:
(a) a nucleic acid sequence which has at least 80% identity to SEQ ID NO 2; wherein said identity can be determined using the DNAsis computer program and default parameters; (b) a nucleic acid which encodes an amino acid sequence which has at least 80% identity to SEQ ID NO 1; wherein said identity can be determined using the DNAsis computer program and default parameters; (c) a nucleic acid sequence which is an allelic variant of SEQ ID NO 2: and (d) a nucleic acid sequence fully complementary to a nucleic acid sequence selected from the group consisting of: a nucleic acid sequence of (a); a nucleic acid sequence of (b); and a nucleic acid sequence of (c).
- 6. A nucleic acid compound which encodes a nematode neuromuscular junction GABA receptor complex subunit, comprising a nucleic acid sequence selected from the group consisting of:
(a) a nucleic acid sequence which has at least 80% identity to SEQ ID NO 4; wherein said identity can be determined using the DNAsis computer program and default parameters; (b) a nucleic acid which encodes an amino acid sequence which has at least 80% identity to SEQ ID NO 3; wherein said identity can be determined using the DNAsis computer program and default parameters; (c) a nucleic acid sequence which is an allelic variant of SEQ ID NO 4; and (d) a nucleic acid sequence fully complementary to a nucleic acid sequence selected from the group consisting of: a nucleic acid sequence of (a); a nucleic acid sequence of (b); and a nucleic acid sequence of (c).
- 7. A nucleic acid compound which encodes a nematode neuromuscular junction GABA receptor complex, comprising a first and second nucleic acid sequence, wherein said first nucleic acid sequence is selected from the group consisting of:
(a) a nucleic acid sequence which has at least 80% identity to SEQ ID NO 2; wherein said identity can be determined using the DNAsis computer program and default parameters; (b) a nucleic acid which encodes an amino acid sequence which has at least 80% identity to SEQ ID NO 1; wherein said identity can be determined using the DNAsis computer program and default parameters; (c) a nucleic acid sequence which is an allelic variant of SEQ ID NO 2; and (d) a nucleic acid sequence which has at least 80% identity to SEQ ID NO 4; wherein said identity can be determined using the DNAsis computer program and default parameters; (e) a nucleic acid which encodes an amino acid sequence which has at least 80% identity to SEQ ID NO 3; wherein said identity can be determined using the DNAsis computer program and default parameters; (f) a nucleic acid sequence which is an allelic variant of SEQ ID NO 4; and wherein said second nucleic acid sequence is selected from the group consisting of: (a) a nucleic acid sequence which has at least 80% identity to SEQ ID NO 4; wherein said identity can be determined using the DNAsis computer program and default parameters; (b) a nucleic acid which encodes an amino acid sequence which has at least 80% identity to SEQ ID NO 3; wherein said identity can be determined using the DNAsis computer program and default parameters; (c) a nucleic acid sequence which is an allelic variant of SEQ ID NO 4; and.
- 8. An isolated antibody selective for a GABA receptor complex of claim 1.
- 9. An isolated antibody selective for a GABA receptor complex of claim 2.
- 10. An isolated antibody selective for a GABA receptor complex subunit of claim 3.
- 11. An isolated antibody selective for a GABA receptor complex subunit of claim 4.
- 12. A method to determine a test substance's ability to interact with a nematode neuromuscular junction GABA receptor complex, comprising contacting a receptor complex of claim 1 with a test substance and determining whether said test substance and said receptor complex interact.
- 13. A method to determine a test substance's ability to interact with a nematode neuromuscular junction GABA receptor complex, comprising contacting a receptor complex of claim 2 with a test substance and determining whether said test substance and said receptor complex interact.
- 14. A method to determine a test substance's ability to interact with a nematode neuromuscular junction GABA receptor complex subunit, comprising contacting a receptor complex subunit of claim 3 with a test substance and determining whether said test substance and said receptor complex subunit interact.
- 15. A method to determine a test substance's ability to interact with a nematode neuromuscular junction GABA receptor complex subunit, comprising contacting a receptor complex subunit of claim 4 with a test substance and determining whether said test substance and said receptor complex subunit interact.
- 16. A method to detect GABA receptors in a test sample, comprising: (a) immobilizing a test sample on a substrate; (b) contacting the test sample with an antibody of claim 8 under conditions suitable for formation of a GABA receptor:antibody complex bound to the substrate; (c) removing non-bound material from the substrate under conditions that retain GABA:antibody complex binding to the substrate; and (d) detecting the presence of the GABA receptor:antibody complex.
- 17. A method to detect GABA receptors in a test sample, comprising: (a) immobilizing a test sample on a substrate; (b) contacting the test sample with an antibody of claim 9 under conditions suitable for formation of a GABA receptor:antibody complex bound to the substrate; (c) removing non-bound material from the substrate under conditions that retain GABA:antibody complex binding to the substrate; and (d) detecting the presence of the GABA receptor:antibody complex.
- 18. A method to detect GABA receptors in a test sample, comprising: (a) immobilizing a test sample on a substrate; (b) contacting the test sample with an antibody of claim 10 under conditions suitable for formation of a GABA receptor:antibody complex bound to the substrate; (c) removing non-bound material from the substrate under conditions that retain GABA:antibody complex binding to the substrate; and (d) detecting the presence of the GABA receptor:antibody complex.
- 19. A method to detect GABA receptors in a test sample, comprising: (a) immobilizing a test sample on a substrate; (b) contacting the test sample with an antibody of claim 11 under conditions suitable for formation of a GABA receptor:antibody complex bound to the substrate; (c) removing non-bound material from the substrate under conditions that retain GABA:antibody complex binding to the substrate; and (d) detecting the presence of the GABA receptor:antibody complex.
- 20. An isolated nucleic acid compound of claim 5 which comprises a nucleic acid sequence selected from the group consisting of: SEQ ID NO 2; SEQ ID NO 4; SEQ ID NO 6; SEQ ID NO 8; SEQ ID NO 10; and SEQ ID NO 12.
- 21. An isolated amino acid compound of claim 3 which comprises a nucleic acid sequence selected from the group consisting of: SEQ ID NO 1; SEQ ID NO 3, SEQ ID NO 5; SEQ ID NO 7; SEQ ID NO 9; SEQ ID NO 11.
Parent Case Info
[0001] The present invention claims priority to U.S. Provisional Patent Application Serial No. 60/107,727, filed Nov. 9, 1998.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60107727 |
Nov 1998 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09436063 |
Nov 1999 |
US |
Child |
10156240 |
May 2002 |
US |