Claims
- 1. An isolated vertebrate UNC-5 protein comprising SEQ ID NO:5, 6, 7 or, 8, or a fragment thereof having vertebrate UNC-5-specific activity.
- 2. An isolated protein according to claim 1, wherein said protein specifically binds a natural netrin protein.
- 3. A recombinant nucleic acid encoding a protein according to claim 1.
- 4. A cell comprising a nucleic acid according to claim 3.
- 5. A method of making an isolated vertebrate UNC-5 protein, comprising steps: introducing a nucleic acid according to claim 3 into a host cell or cellular extract, incubating said host cell or extract under conditions whereby said nucleic acid is expressed as a transcript and said transcript is expressed as a translation product comprising said protein, and isolating said translation product.
- 6. An isolated vertebrate UNC-5 protein made by the method of claim 5.
- 7. An isolated vertebrate unc-5 nucleic acid comprising SEQ ID NO:1 ,2, 3, or 4, or a fragment thereof having at least 24 consecutive bases of SEQ ID NO:1, 2, 3, or 4 and sufficient to specifically hybridize with a nucleic acid having the sequence of the corresponding SEQ ID NO:1, 2, 3, or 4 in the presence of natural C. elegans unc-5 cDNA.
- 8. A method of screening for an agent which modulates the binding of a vertebrate UNC-5 protein to a binding target, said method comprising the steps of:
incubating a mixture comprising:
an isolated protein according to claim 1, a binding target of said protein, and a candidate agent; under conditions whereby, but for the presence of said agent, said protein specifically binds said binding target at a reference affinity; detecting the binding affinity of said protein to said binding target to determine an agent-biased affinity, wherein a difference between the agent-biased affinity and the reference affinity indicates that said agent modulates the binding of said protein to said binding target.
- 9. A method according to claim 8, wherein said binding target is a natural netrin protein.
Government Interests
[0001] The research carried out in the subject application was supported in part by grants from the National Institutes of Health. The government may have rights in any patent issuing on this application.
Divisions (2)
|
Number |
Date |
Country |
Parent |
09306902 |
May 1999 |
US |
Child |
09933261 |
Aug 2001 |
US |
Parent |
08808982 |
Feb 1997 |
US |
Child |
09306902 |
May 1999 |
US |