Claims
- 1. A neuronal nicotinic acetylcholine receptor, expressed recombinantly in a host cell, said receptor having the ability to bind acetylcholine, in the presence or absence of .alpha.-bungarotoxin, and having the ability to effect membrane depolarization of said host cell, said receptor comprising at least one agonist binding subunit and at least one non-agonist binding subunit, wherein said agonist binding subunit is selected from the group consisting of neuronal nicotinic acetylcholine receptor subunits alpha2, alpha3, alpha4, and alpha5, and said non-agonist binding subunit is selected from the group consisting of neuronal nicotinic acetylcholine receptor subunits beta2, beta3, and beta4, provided, however, that when the agonist binding subunit is alpha4, the non-agonist binding subunit is not beta2.
- 2. A neuronal nicotinic acetylcholine receptor of claim 1, wherein said alpha subunit(s) are encoded by alpha neuronal nicotinic acetylcholine receptor subunit gene sequences selected from the group consisting of: pHYP16, ATCC No. 67646, which encodes alpha2; pPCA48, ATCC No. 67642, which encodes alpha3; pPHYA23-1(E)1, ATCC No. 67644, which encodes alpha4.1; pHIP3(E)3, ATCC No. 67645, which encodes alpha4.2; and PC1321, ATCC No. 67652, which encodes alpha5; and said beta subunit(s) are encoded by beta neuronal nicotinic acetylcholine receptor subunit gene sequences selected from the group consisting of: pPCX49, ATCC No. 67643, which encodes beta2; ESD76, ATCC No. 67653, which encodes beta3; and pZPC13, ATCC No. 67893, which encodes beta4, provided, however, that when the alpha subunit is alpha4.1 or alpha4.2, the beta subunit is not beta2.
- 3. A neuronal nicotinic acetylcholine receptor, expressed recombinantly in a lost cell, said receptor having the ability to bind acetylcholine, in the presence or absence of .alpha.-bungarotoxin, and having the ability to effect membrane depolarization or said host cell, said receptor comprising at least one alpha receptor subunit and at least one beta receptor subunit, wherein said alpha receptor subunit(s) are selected from the group consisting of neuronal nicotinic acetylcholine receptor subunits alpha2, alpha3, and alpha4, and said beta receptor subunit(s) are selected from the group consisting of neuronal nicotinic acetylcholine receptor subunits beta2, beta3, and beta4, provided, however, that when the alpha subunit is alpha4, the beta subunit is not beta2.
- 4. A neuronal nicotinic acetylcholine receptor of claim 3, wherein said alpha subunits are encoded by alpha neuronal nicotinic acetylcholine receptor subunit gene sequences selected from the group consisting of: pHYP16, ATCC No. 67646, which encodes alpha2; pPCA48, ATCC No. 67642, which encodes alpha3; pPHYA23-1(E)1, ATCC No. 67644, which encodes alpha4.1; and pHIP3(E)3, ATCC No. 67645, which encodes alpha4.2; and said beta subunit(s) are encoded by beta neuronal nicotinic acetylcholine receptor subunit gene sequences selected from the group consisting of: pPCX49, ATCC No. 67643, which encodes beta2; ESD76, ATCC No. 67653, which encodes beta3; and pZPC13, ATCC No. 67893, which encodes beta4, provided, however, that when the alpha subunit is alpha4.1 or alpha4.2, the beta subunit is not beta2.
- 5. A neuronal nicotinic acetylcholine receptor according to claim 3 comprising agonist binding subunit alpha2 and non-agonist binding subunit beta2.
- 6. A neuronal nicotinic acetylcholine receptor according to claim 3 comprising agonist binding subunit alpha2 and non-agonist binding subunit beta4.
- 7. A neuronal nicotinic acetylcholine receptor according to claim 3 comprising agonist binding subunit alpha3 and non-agonist binding subunit beta2.
- 8. A neuronal nicotinic acetylcholine receptor according to claim 3 comprising agonist binding subunit alpha3 and non-agonist binding subunit beta4.
- 9. A neuronal nicotinic acetylcholine receptor according to claim 3 comprising agonist binding subunit alpha4 and non-agonist binding subunit beta4.
- 10. A neuronal nicotinic acetylcholine receptor subunit protein, expressed recombinantly in a lost cell, comprised of an amino acid sequence selected from the group consisting of those amino acid sequences shown in FIGS. 15C(1) , 15C(2) , 15C(3) (for alpha2); FIG. 19 (for beta3); FIG. 24 (for beta4); and FIG. 25 (for alpha5).
RELATED APPLICATION
This application is a continuation of U.S. Ser. No. 07/664,473, filed 4 Mar. 1991, now abandoned, which is a continuation of U.S. Ser. No. 07/321,384, filed 14 Mar. 1989, now abandoned, which is a continuation in part of U.S. Ser. No. 07/170,295, filed 18 Mar. of 1988, now abandoned.
ACKNOWLEDGMENT
This invention was made with government support under several grants from the National Institutes of Health and the United States Army.
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
4518527 |
Numa |
May 1985 |
|
Non-Patent Literature Citations (6)
Entry |
Mishina, M. et al. "Expression of Functional Acetylcholine receptor . . . " Nature vol. 307, pp. 604-608 Feb. 16, 1984. |
Whiting & Lindstrom, Proc. Natl. Acad. Sci. USA vol. 84, pp. 595-599, Jan. 1987. |
Boulter, J. et al. "Isolation of a cDNA clone coding for a possible neural nicotinic acetylcholine receptor .alpha.-subunit" Nature vol. 319 pp. 368-374 Jan. 30, 1986. |
Goldman, D. et al. "Members of a Nicotine Acetylcholine receptor gene family . . . " Cell vol. 48, pp. Mar. 27, 1987. |
Whiting, P. J. et al. "Functional acetylcholine receptor in PC12 cells . . . " Nature vol. 327 pp. 515-518 Jun. 11, 1987. |
Whiting, Paul & Lindstrom, Jon "Affinity Labelling of Neuronal acetylcholine receptors . . . " FEBS Letters vol. 23(1) pp. 55-60 Mar. 1987. |
Continuations (2)
|
Number |
Date |
Country |
Parent |
664473 |
Mar 1991 |
|
Parent |
321384 |
Mar 1989 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
170295 |
Mar 1988 |
|