Patent Application
20090281049
The present invention relates to a new use of ginsenoside Compound-K in manufacturing medicaments, in particular use in the manufacturing medicaments for prevention or treatment of arthritis.
Chinese patent (CN1570733A) disclosed the structure, the manufacturing method and the use for anti-tumor of ginsenoside Compound-K, which structural formula is as follow:
But there is no any report on the use of ginsenoside Compound-K for prevention or treatment of arthritis.
The object of the present invention is to study the possibility of using of ginsenoside Compound-K in other fields, and provides new use of ginsenoside Compound-K in manufacturing medicaments.
The present invention provides use of ginsenoside Compound-K in the manufacturing medicaments for prevention or treatment of arthritis.
Mice were treated by intravenous injection of ginsenoside Compound-K at the dose of 360 mg/kg, their act was normal and no behavior out of the way was observed.
1. The object of experiment: to observe the effect of the example to be tested on carrageenan-induced acute inflammation in mice
2. Materials and Reagents
1) Medicine: Ginsenoside Compound-K, at the dose of 10 mg/kg, 5 mg/kg, 2.5 mg/kg.
Indomethacin, at the dose of 10 mg/kg.
Carrageenan, concentration 1% made of double distilled water.
2) Animals:
60 Kunming mice.
Body weight: 19-21 g.
Sex: male.
Number of animals in each group: 10.
Temperature in laboratory: 24-26° C.; Relative humidity: 60-70%
3. Methods of Experiment:
Animals were firstly divided into a large dose group: 10 mg/kg, iv, a medium dose group: 5 mg/kg, iv, a small dose group: 2.5 mg/kg, iv, a Indomethacin group: 10 mg/kg, po and a blank control group: physiological saline 10 mg/kg, iv. And then the mice were administered by aforesaid method for 3 days. 1 hour after the last administration, 0.1 ml 1% carrageenan was injected into the plantar surface of the right hind paw in order to induce inflammation. The volume of the right hind paw of the mice was measured by using a hydroplethismometer before inflammation induced and every other one hour thereafter respectively. The difference of the volume of the right hind paw between before inflammation inducement and at the different time points after inflammation inducement was the swell value. Swell rate and inhibition rate were calculated. The differences among groups were compared with t test.
En=swell value at the different time points after inflammation inducement
Eo=swell value before inflammation inducement
C=swell rate of control group
T=swell rate of treatment group
4. Results
The results were shown in table 1. Ginsenoside Compound-K was able to inhibit carrageenan-induced acute inflammation in mice obviously. The most effective inhibition was observed in the large dose group on the 4th hour with inhibition rate 65.66% for the acute inflammation in mice. The inhibition effect of Indomethacin group amounts to the same as the small dose group.
1. The object of experiment: to observe the effect of example to be tested on carrageenan-induced acute inflammation in rats
2. Materials and Reagents
1) Medicine: Ginsenoside Compound-K, at the dose of 10 mg/kg, 5 mg/kg, 2.5 mg/kg.
Indomethacin, at the dose of 1 mg/kg.
Carrageenan, concentration 1% made of double distilled water
2) Animals:
Rat, SD strain.
Body weight: 130-150 g.
Sex: male.
Number of animals in each group: 8.
Temperature in laboratory: 24-26° C.; Relatively humidity: 60-70%
3. Methods of Experiment:
Animals were firstly divided into large dose group: 10 mg/kg, iv, medium dose group: 5 mg/kg, iv, small dose group: 2.5 mg/kg, iv, an Indomethacin group: 1 mg/kg, po and a blank control group: physiological saline 10 mg/kg, iv. The animals were administered by the foregoing method for 3 days. The volume of the right hind paw of the rats was measured using hydroplethismometer before inflammation induced. 1 hour after the last administration, 0.1 ml 1% carrageenan was injected into the plantar surface of the right hind paw in order to induce inflammation. The volume of the right hind paw of the rats was measured by hydroplethismometer every other hour thereafter respectively. Swell rate and inhibition rate were calculated by the same method as above. Differences among groups were compared with t test.
4. Results
The results were shown in table 2. Ginsenoside Compound-K was able to inhibit carrageenan-induced acute inflammation in rats obviously. The most effective inhibition was observed in the large dose group, where the highest inhibition rate may reach 78.23%. The inhibition effect of Indomethacin group was in between the large dose group and the low dose group.
(A) Preventive Effect of Ginsenoside Compound-K on Adjuvant Induced Adjuvant Arthritis in Rats
1. Materials and Reagents
1) Medicine: Ginsenoside Compound K, at the dose of 10 mg/kg, 5 mg/kg, 2.5 mg/kg.
Indomethacin, at the dose of 1 mg/kg.
Tripterygium Wilfordii Hook, at the dose of 1.5 mg/kg.
Freund's Adjuvant Complete, purchased from Sigma Company, was injected into the plantar surface of the right hind paw by 0.1 ml in order to induce inflammation in rats.
2) Animals:
Rat, SD strain.
Body weight: 130-150 g.
Sex: male.
Number of animals in each group: 8.
Temperature in laboratory: 24-26° C.; Relatively humidity: 60-70%.
2. Methods of Experiment:
Animals were firstly divided into large dose group: 10 mg/kg, iv, medium dose group: 5 mg/kg, iv, small dose group: 2.5 mg/kg, iv, Indomethacin group: 1 mg/kg, po, Tripterygium Wilfordii Hook group: 1.5 mg/kg, po and blank control group: physiological saline 10 mg/kg, iv. The volume of the right hind paw of the rats was measured by using hydroplethismometer before inflammation induced. Then 0.1 ml Freund's Adjuvant Complete was injected into the plantar surface of the right hind paw in order to induce inflammation in rats. The rats were grouped by the foregoing division way in the succeeding day, and to be administered for 15 days. The volume of paw was measured in regular intervals. Swell rate and inhibition rate were calculated by the same method as the foregoing. Differences among groups were compared by t test.
3. Results
The results were shown in table 3. Ginsenoside Compound-K was able to prevent Adjuvant Arthritis in rats. The effect of the large dose group was slightly higher than Tripterygium Wilfordii Hook group and amounts to the same as Indomethacin group.
(B) Treatment Effect of Ginsenoside Compound-K on Adjuvant Induced Adjuvant Arthritis in Rats
1. Materials and Reagents
1) Medicine: Ginsenoside Compound-K, at the dose of 10 mg/kg, 5 mg/kg, 2.5 mg/kg.
Indomethacin, at the dose of 1 mg/kg.
Tripterygium Wilfordii Hook, at the dose of 1.5 mg/kg.
Freund's Adjuvant Complete, purchased from Sigma Company, was injected into the plantar surface of the right hind paw by 0.1 ml in order to induce inflammation in rats.
2) Animals:
Rat, SD strain.
Body weight: 130-150 g.
Sex: male.
Number of animals in each group: 8.
Temperature in laboratory: 24-26° C.; Relative humidity: 60-70%
2. Methods of Experiment:
Animals were firstly divided into large dose group: 10 mg/kg, iv, medium dose group: 5 mg/kg, iv, small dose group: 2.5 mg/kg, iv, Indomethacin group: 1 mg/kg, po, Tripterygium Wilfordii Hook group: 1.5 mg/kg, po. and blank control group: physiological saline 10 mg/kg, iv. The volume of the right hind paw of the rats was measured by using hydroplethismometer before inflammation induced. Then 0.1 ml Freund's Adjuvant Complete was injected into the plantar surface of the right hind paw in order to induce inflammation in rats. 20 days after inflammation induced, the rats were grouped by the foregoing division method and to be administered for 8 days. The volume of paw was measured in regular intervals. Swell rate and inhibition rate were calculated with the same method as the foregoing. Differences among groups were compared with t test.
3. Results
The results were shown in table 4. Ginsenoside Compound-K was able to cure Adjuvant Arthritis in rats. The strongest treatment effect was observed in the large dose group, the best effect shown on the 26th day, inhibition rate may arrive at 36.15%, which was obviously higher than Tripterygium Wilfordii Hook group and Indomethacin group respectively.
1. Materials and Reagents
1) Medicine: Ginsenoside Compound-K, at the dose of 10 mg/kg, 5 mg/kg, 2.5 mg/kg.
Enbrel: at the dose of 9 mg/kg by subcutaneous injection every 4 days.
Tripterygium Wilfordii Hook, at the dose of 1.5 mg/kg.
Inflammation-induced agent: Collagen Type II was dissolved in 0.1 mol/L acetic acid to make final concentration be 2 mg/ml collagen solution, and held in 4° C. overnight. The Collagen Type II solution was added to cold Freund's Adjuvant Incomplete by drops (Collagen Type II: Freund's Adjuvant Incomplete=1:1) on the next day, emulsified completely (Type II Collagen solution: Freund's Adjuvant Incomplete=1:1), and the final concentration of Collagen Type II was 1 mg/ml. The Collagen Type II emulsion was reserved in a refrigerator at 4° C.
Immune method: Each rat was immunized by being injected subcutaneously at 5 points on the back with 0.1 ml emulsion in each point, total of 5 ml (contained 0.5 mg Collagen Type II), and immunized again in the same way 7 days later. An emulsion of 0.1 mol/L acetic acid and Freund's Adjuvant Incomplete was injected in control group. The swell values of the arthrosis in right hind ankle and foot were measured respectively by using a hydroplethismometer before injection and 20 days after immunization.
2) Animals:
Rat, SD strain.
Body weight: 140-160 g.
Sex: male.
Number of animals in each group: 8.
Temperature in laboratory: 24-26° C., Relatively humidity: 60-70%
2. Preventive Effect of Ginsenoside Compound-K on Collagen Type II (CII) Induced Arthritis in Rats
2.1 Methods of Experiment:
The Collagen Type II solution, which had been dissolved in acetic acid and held overnight, was adjusted to concentration 2 mg/ml, then added by drops slowly to Freund's Adjuvant Incomplete in proportion of 1:1. After the mixture was emulsified completely, each rat was injected subcutaneously at 5 points on the back with 0.1 ml emulsion in each point (total 0.5 ml in each rat, containing 0.5 mg Collagen Type II) for the first immunization. 7 days later, the second immunization was repeated in the same way. The animals of control group were treated in a similar way, but the immune components did not contain collagen.
The animals were divided into large dose group: Ginsenoside Compound-K, 10 mg/kg, iv, medium dose group: Ginsenoside Compound-K, 5 mg/kg, iv, small dose group: Ginsenoside Compound-K, 2.5 mg/kg, iv, Tripterygium Wilfordii Hook group: 1.5 mg/kg, po-, Enbrel group: 9 mg/kg, sc and blank control group: physiological saline 10 mg/kg, iv. The animals of administration groups were administered at the first immunization and stopped administering 8 days after the first immunization. The swell value of arthrosis in ankle and foot was measured by using hydroplethismometer before immunization and on day 20, 22, 24, 27 and 29 after the first immunization respectively. Swell rate and inhibition rate were calculated by the same method as the foregoing. Differences among groups were compared with t test. When the treatment finished, the arthroses in ankle and foot were taken for pathological examination.
2.2 Results
The results were shown in table 5. Ginsenoside Compound-K were able to prevent Collagen Type II induced arthritis. The strongest effect was observed in the large dose group, which was higher obviously than the Tripterygium Wilfordii Hook group and amounts to the Enbrel group.
3. Treatment Effect of Ginsenoside Compound-K on Collagen Type II (CII) Induced Arthritis in Rats
3.1 Methods of Experiments:
Collagen Type II (CII) and Freund's Adjuvant Incomplete were ground into emulsion and were injected subcutaneously into the back of the rats for immunization. Until the 19th day, most parts of the pedal joint of the rats began to swell. Picked 40 rats out which were with swelled pedal joint and divided them into 5 groups subsequently, i.e. 8 rats in each group. They are respectively inflammation-induced control group: Ginsenoside Compound-K, 10 mg/kg, iv, medium dose group: Ginsenoside Compound-K, 5 mg/kg, iv, small dose group: Ginsenoside Compound-K, 2.5 mg/kg, iv, Enbrel group: 9 mg/kg, sc, Tripterygium Wilfordii Hook group: 1.5 mg/kg, po. and blank control group: physiological saline 10 mg/kg, iv. The rats were administered for 8 days consecutively. The swell value of the foot was measured respectively by using hydroplethismometer before inflammation induced and at different time after the onset of symptoms, then calculated swell rate and prohibition rate, and compared the differences among groups with t test.
3.2 Results
The results were shown in table 6. Ginsenoside Compound-K could be able to cure Collagen Type II induced arthritis. The strongest effect was observed in the large dose group, the best effect was achieved on day 31 with the highest inhibition rate 27.67%, which was higher than Tripterygium Wilfordii Hook group and amount to the Enbrel group.
| Number | Date | Country | Kind |
|---|---|---|---|
| 200610116197.0 | Sep 2006 | CN | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/CN2007/002354 | 8/6/2007 | WO | 00 | 5/26/2009 |
