Claims
- 1. A nicotianamine synthase comprising amino acid sequence shown in SEQ ID NO: 1, or amino acid sequence having deletion in a part thereof, being substituted by the other amino acids or being added with the other amino acids.
- 2. The nicotianamine synthase according to claim 1 wherein said enzyme is originated from barley.
- 3. The nicotianamine synthase according to claim 1 or 2 comprising having amino acid sequence shown in SEQ ID NO: 1, 3, 5, 7, 9, 11 or 13.
- 4. The nicotianamine synthase according to claim 1 wherein said enzyme is originated from Arabidopsis.
- 5. The nicotianamine synthase according to claim 1 or 4 comprising having amino acid sequence shown in SEQ ID NO: 17, 19 or 21.
- 6. The nicotianamine synthase according to claim 1 wherein said enzyme is originated from Oryza sativa.
- 7. The nicotianamine synthase according to claim 1 or 6 comprising having amino acid sequence shown in SEQ ID NO: 15.
- 8. A gene encoding amino acid sequence of nicotianamine synthase according to any one of claims 1-7.
- 9. The gene according to claim 8 wherein said gene is cDNA.
- 10. The gene according to claim 8 or 9 comprising having base sequence shown in SEQ ID NO: 2, 4, 6, 8, 10, 12 or 14.
- 11. The gene according to claim 8 or 9 comprising having base sequence shown in SEQ ID NO: 18, 20 or 22.
- 12. A vector comprising containing gene according to any one of claims 8-11.
- 13. The vector according to claim 12 wherein said vector is an expression vector.
- 14. A transformant wherein said transformant is transformed by the vector according to claim 12 or 13.
- 15. The transformant according to claim 14 wherein the foreign gene is a gene having base sequence shown in SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, or 22.
- 16. The transformant according to claim 14 or 15 wherein the host is bacteria.
- 17. The transformant according to claim 14 or 15 wherein the host is higher bacteria.
- 18. A process for production of nicotianamine comprising using the transformant according to any one of claims 14-17.
- 19. A plant wherein the gene according to any one of claims 8-10 is introduced.
- 20. The plant according to claim 19 wherein said plant is seed.
- 21. A fruit obtained by growing the plant according to claim 19 or 20.
- 22. An antibody against nicotianamine synthase according to any one of claims 1-7.
- 23. The antibody according to claim 22 wherein said antibody is polyclonal antibody.
- 24. The antibody according to claim 22 wherein said antibody is monoclonal antibody.
- 25. A method for extraction of nicotianamine synthase comprising extracting the said enzyme in the presence of thiol protease inhibitor at the extraction of nicotianamine synthase from the plant.
- 26. The method according to claim 25 wherein the thiol protease inhibitor is E-64.
Parent Case Info
[0001] This is a divisional application which claims the benefit of U.S. patent application Ser. No. 09/674,337, filed on Jul. 26, 2001, which is incorporated by reference.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09674337 |
Jul 2001 |
US |
Child |
10281024 |
Oct 2002 |
US |