Patent Application
20070010670
The present invention relates to a nitrogen-containing fused ring compounds and use thereof.
Uric acid is a substance having a molecular weight of 168 and a dissociation constant (pKa value) of 5.75, which is present in the form of uric acid or a conjugate base (urate) thereof in the body fluid depending on the pH of the body fluid. In human, since the function of urate oxidase (uricase) of the liver is lack by mutation, uric acid is the final metabolite of purine form. To be specific, dietarily or endogenously produced purine form becomes inosine from adenosine, then hypoxanthine, and then xanthine, or becomes guanine from guanosine, and then xanthine, and this xanthine is subject to oxidization by xanthine oxidase or xanthine dehydrogenase to become uric acid. Uric acid is mainly excreted from the kidney.
Hyperuricemia becomes severe, and when the blood uric acid level exceeds the upper limit of solubility, sodium urate crystal forms in the cartilage tissues and joints and then sediments called gouty tophus (tophi) are produced. This gouty tophus causes acute gouty arthritis, which progresses into chronic gouty arthritis. Besides these, it has been clarified that nephropathy (gouty kidney) and urolithiasis occur as complications of sodium urate crystal deposition due to hyperuricemia, and that hyperuricemia itself induces renal function disorder (see Johnson R J, Kivilighn S D, Kim Y G et al. Reappraisal of the pathogenesis and consequences of hyperuricemia in hypertension, cardiovascular disease, and renal disease. Am. J. Kidney Dis. 33, 225-234(1999)).
Many hyperuricemia patients have complications such as hyperlipidemia, diabetes, hypertension, obesity and the like. While these complications are each a risk factor for coronary artery disease and death rates, hyperuricemia patients have long been known to show significantly high complication rate of coronary artery diseases and short survival, as compared to patient groups having normal blood uric acid level. Fang et al. conducted a large-scale investigation on the death rates of coronary artery disease in 5926 cases ranging from 25 to 74 years old whose blood uric acid level was measured during the period of 22 years from 1971 to 1992, and clarified that increased blood uric acid level alone can be a risk for ischemic heart diseases (see Fang J., Alderman M H. JAMA 283(18) 2404-2410 (2000)). It has been also reported that a treatment aiming at decreasing the blood uric acid level itself, along with the treatment of complications, is useful for preventing the onset of and decreasing the death rates of coronary artery disease, and a treatment to decrease the blood uric acid level should be positively employed for asymptomatic hyperuricemia, too. (see Tohru Nakamura, Hyperuricemia and Gout, 9(1) 61-65 (2001)). Recently, it has become determinative that hyperuricemia associated with hypertension is an independent risk factor for cardiovascular diseases (see Ichiro Hisatome, Nikkei Medical, January 2004, 100-101). According to Guideline for the management of hyperuricemia and gout (1st Edit.), Gout and Nucleic acid metabolism, vol. 26, suppl. 1 (2002), Japanese Society of Gout and Nucleic Acid Metabolism, moreover, it has been also described that 1) nephropathy (also referred to as gouty kidney) and urolithiasis, which are complications associated with sodium urate crystal deposition, highly frequently occur, 2) control of blood uric acid level is important from the aspect of prevention of a recurrence of cerebral or cardiovascular incidents, 3) patients with hyperuricemia or gout show frequent complication of hyperlipidemia, 4) obesity should not be ignored as etiology or exacerbation factor of hyperuricemia, 5) uricosuric agents are basically used for decreased uric acid excretion hyperuricemia, 6) hyperuricemia associated with hypertension is highly likely an independent risk factor of cardiovascular incidents, and the like. Therefore, it is almost certain that decreasing the blood uric acid level is not the only effective measure for the prophylaxis or treatment of the above-mentioned diseases, but so is combined use of a pharmaceutical agent that decreases the blood uric acid level with therapeutic or prophylactic agents for these above-mentioned diseases.
While uric acid is mainly excreted from the kidney, uric acid in blood is once filtered off almost completely by renal glomerulus, after which uric acid is mostly reabsorbed by proximal renal tubule. Therefore, only a small amount of uric acid is excreted into urine. The proximal reabsorption of uric acid has been clarified to be a transport via a transporter by an experiment using membrane vesicle prepared from renal cortex (see Sica D A and Schoolwerth A C. The Kidney, 6th edition, pp. 680-700, Saunders, Philadelphia (2000)), and its substrate selectivity, inhibitors thereof and the like have also been elucidated (see Roch-Ramel F., Werner D., and Guisan B. Am. J. Physiol. 266, F797-F805 (1994), Roch-Ramel F., Guisan B., and Diezi J. J. Pharmacol. Exp. Ther. 280, 839-845 (1997)).
In recent years, a gene encoding a human kidney uric acid transporter (SLC22A12) has been identified (see Enomoto A. et al., Nature 417, 447-452 (2002)). The transporter (urate transporter 1, URAT1) encoded by this gene is a 12-spanning transmembrane molecule belonging to the organic anion transporter (OAT) family, and Northern blot using the full-length cDNA thereof as a probe has revealed that it specifically expresses in adult and embryo kidneys. It has been also confirmed by immunostaining of human renal tissue section conducted using a polyclonal antibody specific to C-terminal peptide thereof that it is localized on the lumen of the proximal renal tubule in the cortex. In an experiment using a Xenopus laevis oocyte expression system, uric acid uptake via URAT1 increased in a time-dependent manner, and the uric acid uptake showed saturation at high uric acid concentration, which is characteristic of carrier transport. Moreover, it has been clarified that the uptake is based on the exchange with organic anion such as lactic acid, pyrazine carboxylic acid, nicotinic acid and the like, and that the uptake is inhibited by uricosuric agents such as probenecid, benzbromarone and the like, and URAT1 has been demonstrated to be the transporter being elucidated by experiments using the above-mentioned membrane vesicle (see Naohiko Anzai et. al., Biochemical 76(2) 101-110 (2004)). In other words, URAT1 has been clarified to be a main transporter responsible for reabsorption of uric acid in the kidney.
Furthermore, gene mutations of URAT1 have been identified by gene analysis of idiopathic renal hypouricemia patients, and when these mutant URAT1 were expressed in Xenopus laevis oocyte, the uric acid transport activity had been lost. These facts also clarify that URAT1 is involved in the control of blood uric acid level (see Enomoto A. et al., Nature 417, 447-452 (2002)).
With regard to the relationship between URAT1 and diseases, moreover, many reports have documented that probenecid and benzbromarone that inhibit the uric acid transport activity of URAT1 are therapeutic drugs for hyperuricemia, and useful as drugs for the prophylaxis or treatment of pathology exhibiting high blood uric acid level, such as hyperuricemia, gouty tophus, gout arthritis, gouty kidney, urolithiasis and renal function disorder (see Hisashi Yamanaka, Diagnosis and Treatment, vol. 92, No. 1, 125-128 (2004)).
Furthermore, since some of the drugs of nucleic acid metabolic antagonists, hypotensive diuretics, antituberculosis, anti-inflammatory analgesic drugs, hyperlipidemic drugs, therapeutic drugs for asthma, immunosuppressants and the like increase blood uric acid level, thus creating the problems of progress into or exacerbation of pathology caused by increase in the above-mentioned blood uric acid level.
Therefore, a substance having an inhibitory action on URAT1 activity would be useful as a drug for the prophylaxis or treatment of pathology suggesting the involvement of uric acid, such as pathology suggesting the involvement of high blood uric acid level, specifically, hyperuricemia, gouty tophus, gout arthritis, gouty kidney, urolithiasis, renal function disorder and the like, and further as a drug for the prophylaxis or treatment of hyperlipidemia, diabetes, obesity or cardiovascular diseases (e.g., hypertension, coronary arterial disease, vascular endothelial disorder, ischemic heart disease etc.) because it decreases the blood uric acid level. A concurrent use of these other prophylactic or therapeutic drugs with the substance having an inhibitory action on URAT1 activity would be useful for more effective prophylaxis or treatment of these diseases.
A substance having an inhibitory action on URAT1 activity can be said to be useful because it can prevent increase in the blood uric acid level when concurrently used together with a pharmaceutical agent that prevents increase in the blood uric acid level, such as nucleic acid metabolic antagonist, hypotensive diuretic, anti-tuberculosis, anti-inflammatory analgesic drugs, hyperlipidemic drugs, therapeutic drugs for asthma, immunosuppressants and the like.
As a compound having a structure similar to that of the present invention, the following compounds are known. For example, JP-B-7-76214 describes, as a compound showing a vasopressin antagonistic action, a compound represented by the following formula:
wherein R1 is a hydrogen atom, a halogen atom, a lower alkyl group, an amino group optionally having lower alkyl group(s) as substituent(s), or lower alkoxy group, R2 is a hydrogen atom, a halogen atom, a lower alkoxy group, a phenyl-lower alkoxy group, a hydroxyl group, a lower alkyl group, an amino group optionally having lower alkyl group(s) as substituent(s), a carbamoyl-substituted lower alkoxy group, an amino group-substituted lower alkoxy group optionally having lower alkyl group(s) as substituent(s), or a benzoyloxy group optionally having halogen atom(s) on the phenyl ring as substituent(s), R3 is —NR4R5 or —CONR11R12, and W is a group: —(CH2)p- (p is an integer of 3-5) or a group: —CH═CH—(CH2)q- (q is 1 or 2).
However, the compound has a different structure from that of the compound of the present invention, and no description suggestive of the compound of the present invention can be found.
Now, known uricosuric agents and compounds considered to be comparatively similar to the compound of the present invention are described below. For example, U.S. Pat. No 265,559 describes, as a compound showing a uric acid excretion promoting action, the following compound, or benzbromarone.
However, this compound has a different structure from that of the compound of the present invention, and no description suggestive of the compound of the present invention can be found. In addition, this compound is suggested to potentially cause pharmacokinetic drug interaction because it has a strong inhibitory action on CYP2C8, CYP2C9, CYP2C19 and CYP3A4, molecular species of cytochrome P450 (CYP), which is a drug-metabolizing enzyme (see Chiyoko Kunishima et. al., J. Saitama Med School, vol. 30, No. 4, p 287-194 (2004)).
At present, as an agent for the prophylaxis or treatment of hyperuricemia, a uricosuric agent, benzbromarone, having an inhibitory action on URAT1 activity is used. However, the inhibitory action on URAT1 activity of benzbromarone is not sufficient. Moreover, a possibility of inducing a pharmacokinetic drug interaction has been suggested in view of its CYP inhibitory action. Therefore, there is a strong demand for the development of an agent for the prophylaxis or treatment of hyperuricemia, which has more potent inhibitory action on URAT1 activity and has no or very weak CYP inhibitory action. The present invention aims at providing an agent for the prophylaxis or treatment of hyperuricemia, which has more potent inhibitory action on URAT1 activity and has no or very weak CYP inhibitory action, namely, a URAT1 activity inhibitor and the like.
The present inventors have conducted intensive studies in an attempt to develop a new agent for the prophylaxis or treatment of hyperuricemia, which takes the place of conventional agents for the prophylaxis or treatment of hyperuricemia, and found a nitrogen-containing fused ring compound having a superior inhibitory action on URAT1 activity, which resulted in the completion of the present invention.
Accordingly, the present invention provides the following.
<1>
The present inventors have also found a crystal of the above-mentioned compound. Accordingly, the present invention encompasses the following crystal and a pharmaceutical composition comprising the crystal.
<81>
The nitrogen-containing fused ring compound of the present invention effectively inhibits the activity of URAT1. Therefore, it is effective as an agent for the prophylaxis or treatment of pathology showing involvement of uric acid, such as hyperuricemia, gouty tophus, acute gouty arthritis, chronic gouty arthritis, gouty kidney, urolithiasis, renal function disorder, coronary artery disease, ischemic heart disease and the like. Different from conventional agents for the prophylaxis or treatment of hyperuricemia, the compound of the present invention substantially does not inhibit CYP. Therefore, the possibility of inducing a pharmacokinetic drug interaction is extremely slim, which leads to the expectation of the effect of reducing the side effects.
Each substituent used in the present specification is defined in the following.
The “C1-6 alkyl group” is a straight chain or branched chain alkyl group having 1 to 6 carbon atoms and, for example, methyl group, ethyl group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, tert-butyl group, pentyl group, isopentyl group, neopentyl group, tert-pentyl group, hexyl group and the like can be mentioned. Preferred is a C1-4 alkyl group and particularly preferred are methyl group, ethyl group, isopropyl group and tert-butyl group.
The “C1-4 alkyl group” is a straight chain or branched chain alkyl group having 1 to 4 carbon atoms and, for example, methyl group, ethyl group, propyl group, isopropyl group, butyl group, isobutyl group, sec-butyl group, tert-butyl group and the like can be mentioned. Preferred are methyl group, ethyl group, isopropyl group and tert-butyl group.
The “C2-6 alkenyl group” is a straight chain or branched chain alkenyl group having 2 to 6 carbon atoms and, for example, vinyl group, n-propenyl group, isopropenyl group, n-butenyl group, isobutenyl group, sec-butenyl group, n-pentenyl group, isopentenyl group, 1-methylpropenyl group, n-hexenyl group, isohexenyl group, 1,1-dimethylbutenyl group, 2,2-dimethylbutenyl group, 3,3-dimethylbutenyl group, 3,3-dimethylpropenyl group, 2-ethylbutenyl group and the like can be mentioned. Preferred is a straight chain or branched chain alkenyl group having 2 to 4 carbon atoms and particularly preferred are vinyl group, n-propenyl group and isopropenyl group.
The “halogen atom” is fluorine atom, chlorine atom, bromine atom or iodine atom.
The “C1-6 alkoxy group” is an alkoxy group wherein the alkyl moiety is the “C1-6 alkyl group” defined above and, for example, methoxy group, ethoxy group, propoxy group, isopropoxy group, butoxy group, isobutoxy group, sec-butoxy group, tert-butoxy group, pentyloxy group, hexyloxy group and the like can be mentioned. Preferred is an alkoxy group wherein the alkyl moiety is the “C1-4 alkyl group” defined above and particularly preferred are methoxy group and ethoxy group.
The “C1-4 alkoxy group” is an alkoxy group wherein the alkyl moiety is the “C1-4 alkyl group” defined above and, for example, methoxy group, ethoxy group, propoxy group, isopropoxy group, butoxy group, isobutoxy group, sec-butoxy group, tert-butoxy group and the like can be mentioned. Particularly preferred are methoxy group and ethoxy group.
The “saturated or unsaturated carbon ring group having 3 to 14 carbon atoms” is a saturated or unsaturated cyclic hydrocarbon group having 3 to 14 carbon atoms, which is specifically an aryl group, a cycloalkyl group, a cycloalkenyl group, a group derived from a fused carbon ring, wherein two or more of rings constituting them are condensed, and the like. The “aryl group” is an aromatic hydrocarbon group having 6 to 14 carbon atoms and, for example, phenyl group, naphthyl group, biphenyl group, anthryl group, azulenyl group, phenanthryl group, pentalenyl group and the like can be mentioned. Preferred is phenyl group.
The “cycloalkyl group” is a cycloalkyl group having 3 to 8 carbon atoms and, for example, cyclopropyl group, cyclobutyl group, cyclopentyl group, cyclohexyl group, cycloheptyl group, cyclooctyl group and the like can be mentioned. Preferred is a cycloalkyl group having 3 to 6 carbon atoms, which is specifically cyclopropyl group, cyclobutyl group, cyclopentyl group and cyclohexyl group. Particularly preferred are cyclopropyl group and cyclohexyl group.
The “cycloalkenyl group” is a cycloalkenyl group having 3 to 8 carbon atoms, and contains at least one, preferably 1 or 2 double bonds. For example, cyclopropenyl group, cyclobutenyl group, cyclopentenyl group, cyclopentadienyl group, cyclohexenyl group, cyclohexadienyl group (2,4-cyclohexadien-1-yl group, 2,5-cyclohexadien-1-yl group etc.), cycloheptenyl group, cyclooctenyl group and the like can be mentioned. Preferred is a cycloalkenyl group having 3 to 6 carbon atoms, and particularly preferred is cyclohexenyl group.
As the group derived from a fused carbon ring, wherein two or more rings constituting these “aryl group”, “cycloalkyl group” and “cycloalkenyl group” are condensed, for example, indenyl group, indanyl group, fluorenyl group, 1,4-dihydronaphthyl group, 1,2,3,4-tetrahydronaphthyl group (1,2,3,4-tetrahydro-2-naphthyl group, 5,6,7,8-tetrahydro-2-naphthyl group etc.), perhydronaphthyl group and the like can be mentioned.
The “saturated or unsaturated carbon ring having 3 to 14 carbon atoms” is a ring constituting the “saturated or unsaturated carbon ring group having 3 to 14 carbon atoms” defined above.
The “aralkyl group” is an arylalkyl group wherein the aryl moiety is the “aryl group” defined above and the alkyl moiety is the “C1-6 alkyl group” defined above and, for example, benzyl group, phenethyl group, 3-phenylpropyl group, 4-phenylbutyl group, 6-phenylhexyl group and the like can be mentioned. Preferred is an aralkyl group having 7 to 14 carbon atoms and particularly Preferred is benzyl group.
The “aralkoxy group” is an arylalkoxy group wherein the aryl moiety is the “aryl group” defined above and the alkoxy moiety is the “C1-6 alkoxy group” defined above and, for example, benzyloxy group, 3-phenylpropyloxy group, 4-phenylbutyloxy group, 6-phenylhexyloxy group and the like can be mentioned. Preferred is an aralkoxy group having 7 to 14 carbon atoms and particularly Preferred is benzyloxy group.
The “cycloalkylalkoxy group” is a cycloalkylalkoxy group wherein the cycloalkyl moiety is the “cycloalkyl group” defined above and the alkoxy moiety is the “C1-6 alkoxy group” defined above and, for example, cyclopropylmethoxy group, cyclobutylmethoxy group, cyclopentylmethoxy group, cyclohexylmethoxy group and the like can be mentioned. Preferred is a cycloalkylalkoxy group having 4 to 8 carbon atoms and particularly Preferred are cyclopropylmethoxy group and cyclohexylmethoxy group.
The “aryloxy group” is an aryloxy group wherein the aryl moiety is the “aryl group” defined above and, for example, phenoxy group, naphthyloxy group, biphenyloxy group and the like can be mentioned. Preferred is phenoxy group.
The “saturated or unsaturated heterocyclic group containing at least one heteroatom selected from a nitrogen atom, an oxygen atom and a sulfur atom” is a saturated or unsaturated (including partially unsaturated and completely unsaturated) monocyclic 5-membered or 6-membered heterocyclic group, containing, besides carbon atoms, at least one, preferably 1 to 4, heteroatoms selected from a nitrogen atom, an oxygen atom and a sulfur atom; a fused ring group wherein two or more of these heterocycles are condensed; or a fused ring group wherein one of the heterocycles and a carbon ring selected from benzene, cyclopentane and cyclohexane are condensed.
As the “saturated monocyclic 5-membered or 6-membered heterocyclic group”, for example, pyrrolidinyl group, tetrahydrofuryl group, tetrahydrothienyl group, imidazolidinyl group, pyrazolidinyl group, 1,3-dioxolanyl group, 1,3-oxathiolanyl group, oxazolidinyl group, thiazolidinyl group, piperidinyl group, piperazinyl group, tetrahydropyranyl group, tetrahydrothiopyranyl group, dioxanyl group, morpholinyl group, thiomorpholinyl group, 2-oxopyrrolidinyl group, 2-oxopiperidinyl group, 4-oxopiperidinyl group, 2,6-dioxopiperidinyl group and the like can be mentioned.
As the “unsaturated monocyclic 5-membered or 6-membered heterocyclic group”, for example, pyrrolyl group, furyl group, thienyl group, imidazolyl group, 1,2-dihydro-2-oxoimidazolyl group, pyrazolyl group, oxazolyl group, isoxazolyl group, thiazolyl group, isothiazolyl group, triazolyl group (e.g., 1,2,4-triazolyl group, 1,2,3-triazolyl group etc.), tetrazolyl group, 1,3,4-oxadiazolyl group, 1,2,4-oxadiazolyl group, 1,3,4-thiadiazolyl group, 1,2,4-thiadiazolyl group, furazanyl group, pyridyl group, pyrimidinyl group, 3,4-dihydro-4-oxopyrimidinyl group, pyridazinyl group, pyrazinyl group, 1,3,5-triazinyl group, imidazolinyl group, pyrazolinyl group, oxazolinyl group (e.g., 2-oxazolinyl group, 3-oxazolinyl group, 4-oxazolinyl group etc.), isoxazolinyl group, thiazolinyl group, isothiazolinyl group, pyranyl group, 2-oxopyranyl group, 2-oxo-2,5-dihydrofuranyl group, 1,1-dioxo-1H-isothiazolyl group and the like can be mentioned.
As the “fused heterocyclic group”, for example, indolyl group (e.g., 4-indolyl group, 7-indolyl group etc.), isoindolyl group, 1,3-dihydro-1,3-dioxoisoindolyl group, benzofuranyl group (e.g., 4-benzofuranyl group, 7-benzofuranyl group etc.), indazolyl group, isobenzofuranyl group, benzothiophenyl group (e.g., 4-benzothiophenyl group, 7-benzothiophenyl group etc.), benzoxazolyl group (e.g., 4-benzoxazolyl group, 7-benzoxazolyl group etc.), benzimidazolyl group (e.g., 4-benzimidazolyl group, 7-benzimidazolyl group etc.), benzothiazolyl group (e.g., 4-benzothiazolyl group, 7-benzothiazolyl group etc.), indolizinyl group, quinolyl group, isoquinolyl group, 1,2-dihydro-2-oxoquinolyl group, quinazolinyl group, quinoxalinyl group, cinnolinyl group, phthalazinyl group, quinolizinyl group, purinyl group, pteridinyl group, indolinyl group, isoindolinyl group, 5,6,7,8-tetrahydroquinolyl group, 1,2,3,4-tetrahydroquinolyl group, 2-oxo-1,2,3,4-tetrahydroquinolyl group, benzo[1,3]dioxolyl group, 3,4-methylenedioxypyridyl group, 4,5-ethylenedioxypyrimidinyl group, chromenyl group, chromanyl group, isochromanyl group and the like can be mentioned.
The “monocyclic nitrogen-containing saturated heterocycle” formed together with the adjacent nitrogen atom is a saturated 5-membered or 6-membered monocyclic heterocycle containing at least one nitrogen atom, such as piperidine, morpholine, piperazine, pyrrolidine and the like.
Being “optionally substituted by one or more, the same or different substituents” means being unsubstituted or being substituted by at least one to the acceptable maximum number of substituents. In the case of a methyl group, for example, it means being optionally substituted by 1 to 3 substituents, and in the case of an ethyl group, it means being optionally substituted by 1 to 5 substituents. When substituted by 2 or more substituents, the substituents may be the same or different and the position of the substituents may be any, without any particular limitation. Preferred is being “optionally substituted by the same or different, 1 to 3 substituents”.
Preferable examples of each group are as follows.
R1, R2 and R3 are preferably the same or different and each is
R1, R2 and R3 are more preferably the same or different and each is
For each of R1, R2 and R3, the following are more preferable.
R1 is more preferably
R2 is more preferably
R3 is more preferably
R1, R2 and R3 are particularly preferably the same or different and each is
For each of R1, R2 and R3, the following are particularly preferable.
R1 is particularly preferably
R2 is particularly preferably
R3 is particularly preferably
X1 is preferably
X1 is more preferably
X2 is preferably
X2° is preferably
X2′ is more preferably an oxygen atom.
—X3—X4— is preferably
As —(CR11R12)n-,
—X3—X4— is more preferably —CH2—CH2—.
Ring A is preferably an unsaturated carbon ring group having 3 to 14 carbon atoms optionally substituted by one or more, preferably 2 or 3, the same or different substituents selected from the aforementioned group A, or an unsaturated heterocyclic group containing at least one heteroatom selected from a nitrogen atom, an oxygen atom and a sulfur atom, optionally substituted by one or more, preferably 2 or 3, the same or different substituents selected from the aforementioned group A.
Ring A is more preferably
wherein R23 to R27 are the same or different and each is
R23 to R27 on ring A are preferably are the same or different and each is
R23 to R27 on ring A are more preferably the same or different and each is
For each of R23 to R27 on ring A, the following are more preferable.
R23 on ring A is more preferably
R24 on ring A is more preferably
R25 on ring A is more preferably
R26 on ring A is more preferably
R27 on ring A is more preferably
As such ring A,
and the like can be mentioned.
R23 to R27 on ring A are particularly preferably the same or different and each is
For each of R23 to R27 on ring A, the following are particularly preferable.
R23 on ring A is particularly preferably
R24 on ring A is particularly preferably
R25 on ring A is particularly preferably
R26 on ring A is particularly preferably
R27 on ring A is particularly preferably
Ring A′ is preferably an unsaturated carbon ring group having 3 to 14 carbon atoms optionally substituted by one or more, preferably 2 or 3, the same or different substituents selected from the aforementioned group C, or an unsaturated heterocyclic group containing at least one heteroatom selected from a nitrogen atom, an oxygen atom and a sulfur atom, optionally substituted by one or more, preferably 2 or 3, the same or different substituents selected from the aforementioned group C. Ring A′ is substituted by at least one —OR13′ wherein R13′ is as defined above.
Ring A′ is preferably
wherein
1) a hydrogen atom, or
2) a group selected from the aforementioned group C, and at least one of R23 to R27 is —OR13′ wherein R13′ is as defined above.
R23 to R27 on ring A′ are preferably are the same or different and each is
R23 to R27 on ring A′ are more preferably the same or different and each is
For each of R23 to R27 on ring A′, the following are more preferable.
R23 on ring A′ is more preferably
R24 on ring A′ is more preferably
R25 on ring A′ is more preferably
R26 on ring A′ is more preferably
R27 on ring A′ is more preferably
As such ring A′,
and the like can be mentioned.
R23 to R27 on ring A′ are particularly preferably the same or different and each is
For each of R23 to R27 on ring A′, the following are particularly preferable.
R23 on ring A′ is particularly preferably
R24 on ring A′ is particularly preferably
R25 on ring A′ is particularly preferably
R26 on ring A′ is particularly preferably
R27 on ring A′ is particularly preferably
Of compounds [1], compound [2] is preferable. Particularly, a compound wherein
Particularly, a compound wherein
The compound selected from the following group or a pharmaceutically acceptable salt thereof is particularly preferable.
The nitrogen-containing fused ring compounds of the present invention are divided into in the following 6 types (type A-type F) depending on the kind of X2′. Preferable compounds for each are shown in the following.
type A
Of compounds [2], type A is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
Of compounds [2], type B is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
Of compounds [2], type C is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
Of compounds [2], type D is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
Of compounds [2], type E is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
Of compounds [2], type F is a compound wherein
Of these, a compound wherein
Of these, a compound wherein
Particularly, a compound wherein
The nitrogen-containing fused ring compound of the present invention can also be obtained as a crystal. For example,
The “pharmaceutically acceptable salt thereof” may be any as long as it forms nontoxic salts with compounds [1] and [2] (hereinafter they are also to be collectively referred to as the compound of the present invention) and, for example, salts with inorganic acids such as hydrochloric acid, sulfuric acid, phosphoric acid, hydrobromic acid and the like; salts with organic acids such as oxalic acid, malonic acid, citric acid, fumaric acid, lactic acid, malic acid, succinic acid, tartaric acid, acetic acid, trifluoroacetic acid, gluconic acid, ascorbic acid, methanesulfonic acid, benzenesulfonic acid and the like; salts with inorganic bases such as sodium hydroxide, potassium hydroxide, calcium hydroxide, magnesium hydroxide, ammonium hydroxide and the like; salts with organic bases such as methylamine, diethylamine, triethylamine, triethanolamine, ethylenediamine, tris(hydroxymethyl)methylamine, guanidine, choline, cinchonine and the like; salts with amino acids such as lysine, arginine, alanine and the like, and the like can be mentioned.
The present invention also encompasses water-containing products, hydrates and solvates of the compound of the present invention.
In addition, the compound of the present invention has various isomers. For example, E form and Z form can be present as geometric isomers, when an asymmetric carbon atom is present, enantiomer and diastereomer are present as stereoisomers based thereon, and tautomers can also be present. Therefore, the present invention encompasses all of these isomers and mixtures thereof. Moreover, the present invention encompasses, besides the compound of the present invention, prodrug compounds and metabolite compounds of these compounds as equivalent compounds.
As used herein, the “prodrug” is a derivative having a chemically or metabolically decomposable group, which shows a pharmaceutical activity upon decomposition by hydrolysis or solvolysis, or under physiological conditions. A prodrug is used for, for example, improving absorption by oral administration or targeting the object site. Inasmuch as what the chemically or metabolically decomposable group is, and how to introduced the group into a compound have been sufficiently established in the field of pharmaceutical agents, such known techniques can be employed in the present invention. As the moiety to be modified for producing a prodrug, for example, highly reactive functional groups such as a hydroxyl group, a carboxyl group, an amino group, a thiol group and the like in the compound of the present invention can be mentioned. For example, the compound of the present invention represented by the formula [2] wherein —OR13 or —OR13′ is a hydroxyl group and the like can be mentioned.
For example, a derivative wherein a substituent such as —CO—C1-6 alkyl group, —CO2—C1-6 alkyl group, —CONH—C1-6 alkyl group, —CO—C2-6 alkenyl group, —CO2—C2-6 alkenyl group, —CONH—C2-6 alkenyl group, —CO-aryl group, —CO2-aryl group, —CONH-aryl group, —CO-heterocyclic group, —CO2-heterocyclic group, —CONH-heterocyclic group (the C1-6 alkyl group, C2-6 alkenyl group, aryl group and heterocyclic group are each optionally substituted by halogen atom, C1-6 alkyl group, hydroxyl group, C1-6 alkoxy group, carboxy group, amino group, amino acid residue, —PO3H2, —SO3H, —CO-polyethylene glycol residue, —CO2-polyethylene glycol residue, —CO-polyethylene glycol monoalkyl ether residue, —CO2-polyethylene glycol monoalkyl ether residue and the like) and the like has been introduced into a hydroxyl group can be mentioned.
In addition, a derivative wherein a substituent such as —CO—C1-6 alkyl group, —CO2—C1-6 alkyl group, —CO—C2-6 alkenyl group, —CO2—C2-6 alkenyl group, —CO-aryl group, —CO2-aryl group, —CO-heterocyclic group, —CO2-heterocyclic group (the C1-6 alkyl group, C2-6 alkenyl group, aryl group and heterocyclic group are each optionally substituted by halogen atom, C1-6 alkyl group, hydroxyl group, C1-6 alkoxy group, carboxy group, amino group, amino acid residue, —PO3H2, —SO3H, —CO-polyethylene glycol residue, —CO2-polyethylene glycol residue, —CO-polyethylene glycol monoalkyl ether residue, —CO2-polyethylene glycol monoalkyl ether residue, —PO3H2 and the like) and the like has been introduced into an amino group can be mentioned.
Furthermore, a derivative wherein a substituent such as C1-6 alkoxy group, aryloxy group (the C1-6 alkoxy group and aryloxy group are each optionally substituted by halogen atom, C1-6 alkyl group, hydroxyl group, C1-6 alkoxy group, carboxy group, amino group, amino acid residue, —PO3H2, —SO3H, polyethylene glycol residue, polyethylene glycol monoalkyl ether residue and the like) and the like has been introduced into a carboxyl group can be mentioned.
The “pharmaceutical composition” includes, besides what is called a “composition” comprising an active ingredient as a pharmaceutical agent and a pharmaceutically acceptable carrier and the like, a combination agent with other pharmaceutical agents, and the like. It is needless to say that the pharmaceutical composition of the present invention can be concurrently used with any other pharmaceutical agent within the range acceptable in clinical situations. Therefore, the present pharmaceutical composition can also be considered a pharmaceutical composition to be combined with other pharmaceutical agents.
Moreover, the pharmaceutical composition of the present invention can be administered to not only human but also other mammals (mouse, rat, hamster, rabbit, cat, dog, bovine, sheep, monkey etc.). Therefore, the pharmaceutical composition of the present invention is also useful as a pharmaceutical product for animals, not to mention human.
To not “substantially inhibit CYP” means that the function of the drug metabolizing enzyme, cytochrome P450 (CYP), preferably CYP2D6, CYP2C8, CYP2C9, CYP2C19 or CYP3A4, more preferably CYP2C9, is not substantially inhibited and that, for example, the concentration of a substance that dose not substantially inhibit CYP necessary for inhibiting CYP by 50% is not less than 1 μM, preferably not less than 3 μM, more preferably not less than 10 μM, still more preferably not less than 25 μM, particularly preferably not less than 50 μM, based on the conditions of the below-mentioned Experimental Example 2, preferably under the conditions of Experimental Example 2.
To “inhibit URAT1 activity” means to specifically inhibit the function of URAT1 as a uric acid transporter to eliminate or attenuate the activity and means, for example, to specifically inhibit the function of URAT1 based on the conditions of the below-mentioned Experimental Example 1 and preferably means that the concentration necessary for inhibiting URAT1 by 50% is less than 3 μM, more preferably less than 1 μM, more preferably less than 0.3 μM, still more preferably less than 0.1 μM, yet more preferably less than 0.03 μM, under the conditions of the below-mentioned Experimental Example 1. The URAT1 activity inhibitor does not include biological substrates of URAT1, such as uric acid and the like.
To “decrease the blood uric acid level” means to decrease uric acid (including uric acid salt) in blood (including in serum and plasma), preferably to decrease high blood uric acid level, more preferably serum uric acid level, to less than 8 mg/dL (preferably less than 7 mg/dl, more preferably less than 6 mg/dL, as serum uric acid level).
The “pathology showing involvement of uric acid” means pathology in which uric acid (including uric acid salt) in the body such as blood (including in serum and plasma) or urine is involved. Specifically, pathology caused by high blood (including in serum and plasma) uric acid level or urine uric acid level such as hyperuricemia, gouty tophus, acute gouty arthritis, chronic gouty arthritis, gouty kidney, urolithiasis, renal function disorder, coronary artery disease, ischemic heart disease and the like can be mentioned.
For example, the “high blood uric acid level” means a serum uric acid level of not less than 6 mg/dL, preferably not less than 7 mg/dL, more preferably not less than 8 mg/dL. The “pathology caused by high blood uric acid level” is pathology caused by high blood uric acid level, or to which high blood uric acid level contributes. For example, according to “Guideline for the management of hyperuricemia and gout (1st Edit.)” (Gout and Nucleic Acid Metabolism, vol. 26, suppl. 1 (2002), Japanese Society of Gout and Nucleic Acid Metabolism), 7 mg/dL, which is a dissolution concentration of uric acid in plasma, is the normal upper limit, irrespective of sex•age, and any value exceeding this level is defined to be hyperuricemia, and concludes that the serum uric acid level should be desirably controlled to not more than 6 mg/dL for the treatment of hyperuricemia or gout and for the prevention of the onset of gout arthritis.
As the “pharmaceutically acceptable carrier”, various organic or inorganic carrier substances conventionally used as preparation materials are employed, which are added as excipient, lubricant, binder, disintegrant, solvent, dissolution aids, suspending agent, isotonicity agent, buffer, soothing agent and the like. Where necessary, preparation additives such as preservative, antioxidant, sweetening agent, coloring agent and the like can also be used. As preferable examples of the above-mentioned excipient, lactose, sucrose, D-mannitol, starch, crystalline cellulose, light silicic anhydride and the like can be mentioned. As preferable examples of the above-mentioned lubricant, magnesium stearate, calcium stearate, talc, colloidal silica and the like can be mentioned. As preferable examples of the above-mentioned binder, polymer compound such as crystalline cellulose, sucrose, D-mannitol, dextrin, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinylpyrrolidone and the like can be mentioned. As preferable examples of the above-mentioned disintegrant, starch, carboxymethylcellulose, carboxymethylcellulose calcium, croscarmellose sodium, sodium carboxymethyl starch and the like can be mentioned. As preferable examples of the above-mentioned solvent, water for injection, alcohol, propylene glycol, macrogol, sesame oil, corn oil, propylene glycol esters of fatty acids and the like can be mentioned. As preferable examples of the above-mentioned dissolution aids, polyethylene glycol, propylene glycol, D-mannitol, benzyl benzoate, ethanol, trisaminomethane, cholesterol, triethanolamine, sodium carbonate, sodium citrate and the like can be mentioned. As preferable examples of the above-mentioned suspending agent, surfactants such as stearyl triethanolamine, sodium lauryl sulfate, lauryl aminopropionic acid, lecithin, benzalkonium chloride, benzethonium chloride, glyceryl monostearate and the like; polyvinyl alcohol, polyvinylpyrrolidone, carboxymethylcellulose sodium, methylcellulose, hydroxymethylcellulose and the like can be mentioned. As preferable examples of the above-mentioned isotonicity agent, sodium chloride, glycerol, D-mannitol and the like can be mentioned. As preferable examples of the above-mentioned buffer, buffers such as phosphate, acetate, carbonate, citrate and the like, and the like can be mentioned. As preferable examples of the above-mentioned soothing agent, benzyl alcohol and the like can be mentioned. As preferable examples of the above-mentioned preservative, paraoxybenzoates, chlorobutanol, benzyl alcohol, phenethyl alcohol, dehydroacetic acid, sorbic acid and the like can be mentioned. As preferable examples of the above-mentioned antioxidant, sulfite, ascorbic acid and the like can be mentioned. As preferable examples of the above-mentioned sweetening agent, aspartam, saccharin sodium, stevia and the like can be mentioned. As preferable examples of the above-mentioned coloring agent, foodcolors such as Food Color Yellow No. 5, Food Color Red No. 2 and Food Color Blue No. 2 and the like, food lake colors, iron oxide and the like can be mentioned.
The compound of the present invention can be contained as an active ingredient of a pharmaceutical composition, a URAT1 activity inhibitor, an agent for lowering a blood uric acid level, or an agent for the prophylaxis or treatment of pathology showing involvement of uric acid, along with a pharmaceutically acceptable carrier.
When the compound of the present invention is used as the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the drug for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention, it can be systemically or topical, and orally or parenterally administered. While the dose varies depending on the age, body weight, condition, treatment effect and the like, for example, it can be generally administered within the range of 0.1 mg to 1 g per administration to an adult once to several times a day. The compound of the present invention can also be used as a drug for the treatment or prophylaxis of the aforementioned diseases in, not to mention human, animals other than human, particularly mammals. The same applies when the compound of the present invention is used as a drug for the prophylaxis or treatment of hyperlipidemia, diabetes, obesity or cardiovascular disease, such as hypertension, coronary artery disease, vascular endothelial disorder, ischemic heart disease and the like.
To produce a preparation of the compound of the present invention such as a solid composition or liquid composition for oral administration, or an injection for parenteral administration and the like, the compound can be admixed additives such as a suitable diluent, a dispersing agent, an adsorbent, a solubilizer and the like. In addition, the pharmaceutical composition of the present invention may have known form such as tablet, pill, powder, granule, suppository, injection, eye drop, liquid, capsule, troche, aerosol, elixir, suspension, emulsion, syrup and the like.
When the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention is, for example, a solid preparation such as tablet, pill, powder, granule etc., the additive includes, for example, lactose, mannitol, glucose, hydroxypropylcellulose, microcrystalline cellulose, starch, polyvinylpyrrolidone, magnesium aluminosilicate, silicic anhydride powder and the like. When a tablet or pill is to be prepared, sucrose, gelatin, hydroxypropylcellulose, or a film coating of gastrosoluble or enteric substance such as hydroxymethylcellulose phthalate etc. may be applied as necessary, or a multi-layer tablet having two or more layers may be produced.
As the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention, capsules obtained by dissolving the compound of the present invention in a solvent, adding an additive to give, a liquid, semi-solid or solid content and packing the content therein can also be mentioned. As the aforementioned solvent, for example, purified water, ethanol or vegetable oil and the like can be mentioned. Of these, ethanol or a mixture of purified water and ethanol is preferably used. As the additive, additives generally used for the production of capsules can be used without any particularly limitation. As the aforementioned additive, for example, propylene glycol esters of fatty acids; low molecular weight polyethylene glycol such as polyethylene glycol 200-600, low molecular weight polyethylene glycol such as polyethylene glycol 200-600 etc., esters of fatty acids, middle chain fatty acid triglyceride; alcohol/polyhydric alcohols such as stearyl alcohol, cetanol, polyethylene glycol etc., and esters thereof; fats and oils such as sesame oil, soy bean oil, peanut oil, corn oil, hydrogenated oil, paraffin oil, white beeswax etc.; organic carboxylic acid or fatty acid such as triethyl citrate, triacetine, stearic acid, palmitic acid, myristic acid etc. and derivatives thereof and the like can be mentioned, which are suitable for the production of liquid or semi-solid contents. For capsules of the present invention, propylene glycol esters of fatty acids are preferably used as additive. As propylene glycol esters of fatty acids, for example, propylene glycol monocaprylate (Capmul PG-8 (trade name), Sefol218 (trade name), Capryol90 (trade name)), propylene glycol monolaurate (Lauroglycol FCC (trade name)), propylene glycol monooleate (Myverol P-06 (trade name)), propylene glycol myristate, propylene glycol monostearate, propylene glycol ricinoleate (Propymuls (trade name)), propylene glycol dicaprylate/dicaprate (Captex (trademark) 200 (trade name)), propylene glycol dilaurate, propylene glycol distearate, and propylene glycol dioctanoate (Captex (trademark) 800 (trade name)) can be mentioned. While the material constituting the capsule of the present invention is not particularly limited, natural polysacchalides such as agar, alginate, starch, xanthan, dextran etc.; protein such as gelatin, casein etc.; chemically processed products such as hydroxystarch, pullulan, hydroxypropylcellulose, hydroxypropylmethylcellulose, polyvinyl alcohol and derivatives thereof, polyacryl derivative, polyvinylpyrrolidone and derivatives thereof, polyethylene glycol and the like can be mentioned.
When the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention is, for example, a liquid preparation for oral administration of a pharmaceutically acceptable suspending agent, a solubilizer, a suspension, a syrup, an elixir etc., as the diluent to be used, for example, purified water, ethanol, vegetable oil, emulsifier and the like can be mentioned. This liquid preparation may contain, besides the diluent, an auxiliary agent such as an infiltration, a suspension, a sweetening agent, a flavor, an aromatic, a preservative and the like.
When the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention is a parenteral preparation such as injection etc., sterile aqueous or non-aqueous solution, solubilizer, suspension, emulsifier and the like are used as additives. As the aqueous solution, solubilizer and suspension, for example, distilled water for injection; saline, cyclodextrin and derivatives thereof; organic amines such as triethanolamine, diethanolamine, monoethanolamine, triethylamine and the like, inorganic alkali solution and the like can be mentioned. When a water-soluble solution is used, for example, propylene glycol, polyethylene glycol or vegetable oil such as olive oil, alcohols such as ethanol, and the like may be further added. As the solubilizer, for example, surfactants such as polyoxyethylene hydrogenated castor oil, sucrose esters of fatty acids and the like (for forming mixed micelle), lecithin or hydrogenated lecithin (for forming liposome) and the like can also be used. In addition, the parenteral preparation of the present invention can be produced as an emulsion preparation containing water-insoluble solubilizer such as vegetable oil etc., lecithin, polyoxyethylene hydrogenated castor oil, polyoxyethylenepolyoxypropylene glycol and the like.
The compound, pharmaceutical composition, URAT1 activity inhibitor, agent for lowering a blood uric acid level, or agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention can be used in combination with other pharmaceutical composition or pharmaceutical agent (hereinafter to be also referred to as a concomitant drug).
The “use in combination” means use of multiple pharmaceutical agents in combination as active ingredients, and use as a combination drug, use as a kit, use in a combination characterized by independent administration of each by the same or different administration routes and the like can be mentioned.
The administration time of the compound, the pharmaceutical composition, the URAT1 activity inhibitor, the agent for lowering a blood uric acid level, or the agent for the prophylaxis or treatment of pathology showing involvement of uric acid of the present invention and a concomitant drug is not limited, and these may be administered simultaneously to the subject of administration or administered in a staggered manner. The dose of the concomitant drug can be determined according to the dose employed clinically, and appropriately determined depending on the subject of administration, the age and body weight of the subject of administration, condition, administration time, dosage form, administration method, combination and the like. The administration mode of the concomitant drug is not particularly limited, and it is only necessary to combine the compound of the present invention, a pharmaceutical composition, a URAT1 activity inhibitor, an agent for lowering a blood uric acid level, or an agent for the prophylaxis or treatment of pathology showing involvement of uric acid and a concomitant drug at the time of administration.
As the concomitant drug,
As the “agent for the prophylaxis and/or treatment of hyperuricemia”, for example, uric acid production suppressants such as xanthine oxidase inhibitor etc., uricosuric agents and the like can be mentioned, and allopurinol, probenecid, bucolome, febuxostat, benzbromarone, oxipurinol and the like can be specifically mentioned.
As the “agent for the prophylaxis and/or treatment of gout arthritis”, for example, NSAIDs such as indomethacin, naproxen, fenbufen, pranoprofen, oxaprozin and the like, colchicine, corticosteroid and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of gouty kidney”, for example, uric acid production suppressants such as xanthine oxidase inhibitor etc., uricosuric agents, alkalinizing urine agents such as citric acid preparation, sodium bicarbonate etc., and the like can be mentioned, and allopurinol, probenecid, bucolome, febuxostat, benzbromarone and oxipurinol can be specifically mentioned.
As the “agent for the prophylaxis and/or treatment of urolithiasis”, for example, alkalinizing urine agents such as citric acid preparation, sodium bicarbonate etc., and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of hypertension or hypertensive complications”, for example, loop diuretics, angiotensin-convertase inhibitors, angiotensin II receptor antagonists, Ca antagonists, β blockers, α,β blockers, α blockers and the like can be mentioned. More specifically, for example, sustained-release furosemide preparation, captopril, sustained-release captopril preparation, enalapril maleate, alacepril, delapril hydrochloride, cilazapril, lisinopril, benazepril hydrochloride, imidapril hydrochloride, temocapril hydrochloride, quinapril hydrochloride, trandolapril, perindopril erbumine, losartan potassium, candesartan cilexetil, nicardipine hydrochloride, sustained-release nicardipine hydrochloride preparation, nilvadipine, nifedipine, sustained-release nifedipine preparation, benidipine hydrochloride, diltiazem hydrochloride, sustained-release diltiazem hydrochloride preparation, nisoldipine, nitrendipine, manidipine hydrochloride, barnidipine hydrochloride, efonidipine hydrochloride, amlodipine besylate, felodipine, cilnidipine, aranidipine, propranolol hydrochloride, sustained-release propranolol hydrochloride preparation, pindolol, sustained-release pindolol preparation, indenolol hydrochloride, carteolol hydrochloride, sustained-release carteolol hydrochloride preparation, bunitrolol hydrochloride, sustained-release bunitrolol hydrochloride preparation, atenolol, acebutolol hydrochloride, metoprolol tartrate, sustained-release metoprolol tartrate preparation, nipradilol, penbutolol sulfate, tilisolol hydrochloride, carvedilol, bisoprolol fumarate, betaxolol hydrochloride, celiprolol hydrochloride, bopindolol malonate, bevantolol hydrochloride, labetalol hydrochloride, arotinolol hydrochloride, amosulalol hydrochloride, prazosin hydrochloride, terazosin hydrochloride, doxazosin mesilate, bunazosin hydrochloride, sustained-release bunazosin hydrochloride preparation, urapidil, phentolamine mesylate and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of hyperlipidemia or hyperlipidemic complications”, for example, statin pharmaceutical agents, anion exchange resins, probucol, nicotinic acid preparations, fibrate pharmaceutical agents, eicosapentaenoic acid preparations and the like can be mentioned. More specifically, for example, lovastatin, simvastatin, pravastatin, fluvastatin, atorvastatin, cerivastatin, colestimide, colestyramine, niceritrol, nicomol, fenofibrate, bezafibrate, clinofibrate, clofibrate, ethyl icosapentate and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of diabetes or diabetic complications”, for example, insulin preparations, sulfonylureas, insulin secretagogues, sulfonamides, biguanides, a glucosidase inhibitors, insulin sensitizers, angiotensin-convertase inhibitors, aldose reductase inhibitors, antiarrhythmic drugs and the like can be mentioned. More specifically, for example, insulin, chlorpropamide, glibenclamide, glipizide, tolbutamide, glyclopyramide, acetohexamide, glimepiride, tolazamide, gliclazide, nateglinide, glybuzole, metformin hydrochloride, buformin hydrochloride, voglibose, acarbose, pioglitazone hydrochloride, mexiletine and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of obesity or obesity complications”, for example, mazindol, acarbose, voglibose, orlistat and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of primary disease causing decreased uric acid excretion secondary hyperuricemia”, for example, agents for the prophylaxis or treatment of chronic renal disease, polycyctic kidney, toxemia of pregnancy, lead nephropathy, hyperlactacidemia, Down's syndrome, sarcoidosis, glycogenosis I type (via hyperlactacidemia), dehydrating etc., and the like can be mentioned.
As the “agent for the prophylaxis and/or treatment of kidney failure, cardiovascular disorder, cerebrovascular disorder caused by hyperuricemia”, for example, loop diuretics (e.g., furosemide), citric acid preparations, sodium bicarbonate, cation exchange resins, aluminum hydroxide, alfacalcidol, β-blockers (e.g., propranolol hydrochloride), ACE inhibitors (e.g., captopril), cardiac stimulants (e.g., digoxin), angina pectoris therapeutic agents (e.g., isosorbide nitrate), Ca antagonists (e.g., diltiazem hydrochloride), uric acid production suppressants (e.g., allopurinol), amino acid preparations, hyperammonemia improvers, therapeutic agents for antiarrhythmic (e.g., mexiletine), therapeutic agents for anemia (e.g., mepitiostane, erythropoietin), other “agents for the prophylaxis and/or treatment of hypertension or hypertensive complications”, “agents for the prophylaxis and/or treatment of hyperlipidemia or hyperlipidemic complications”, “agents for the prophylaxis and/or treatment of diabetes or diabetic complications”, “agents for the prophylaxis and/or treatment of obesity or obesity complications” and the like can be mentioned.
As the “nucleic acid metabolic antagonist”, for example, azathiopurine, mizoribine, mycophenolic acid and the like can be mentioned.
Furthermore, the compound of the present invention, a pharmaceutical composition, a URAT1 activity inhibitor, an agent for lowering a blood uric acid level, and an agent for the prophylaxis or treatment of pathology showing involvement of uric acid can be used in combination with a pharmaceutical agent that increases the blood uric acid level, thereby to suppress increase in the blood uric acid level.
As the “pharmaceutical agent that increases the blood uric acid level”, nucleic acid metabolic antagonists, hypotensive diuretics, anti-tuberculosis, anti-inflammatory analgesic drugs, hyperlipidemic drugs, therapeutic drugs for asthma, immunosuppressants, salicylic acid, pyrazinamide, ethambutol, nicotinic acid, ethanol, cyclosporine and the like can be mentioned.
The compound of the crystal of the present invention (hereinafter to be sometimes abbreviated as the crystal of the present invention) only needs to be contained in a pharmaceutical composition, and it preferably contains substantially most of the crystal of the present invention.
By “contains substantially most of the crystal of the present invention” is meant that the crystal of the compound of the present invention is contained in a proportion of not less than 80%, preferably not less than 90%, more preferably not less than 95%.
The production methods of the compound of the present invention are specifically explained below. It is needless to say that the present invention is not limited to the production methods below. For production of the compound of the present invention, the order of the reaction can be appropriately changed. The reaction only needs to be carried out from a step or a position that seems to be reasonable.
In addition, a step for appropriately changing substituents (change or further modification of substituents) may be inserted between respective steps. When a reactive functional group is involved, appropriate protection or deprotection may be conducted. To promote progress of the reaction, moreover, reagents other than those exemplified can be appropriately used. The starting material compounds, for which a production method is not described, are commercially available or can be easily produced by combining known synthetic reactions.
The compound obtained in each step can be isolated and purified by conventional methods such as crystallization, recrystallization, column chromatography, preparative HPLC and the like. In some cases, it is possible to proceed to the next step without isolation and purification.
In the following production methods, the “room temperature” means 1-40° C.
Production Method 1
A compound [1] wherein X2 is an oxygen atom and Y is C═O can be produced by the following Steps:
wherein each symbol is as defined above.
Step 1
Compound 2 can be obtained by amidating compound 1 with acid halide B in a solvent in the presence of a base.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethyl sulfoxide, water and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate, water and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned, with preference given to triethylamine, pyridine, sodium hydroxide and sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 80° C., preferably about 0° C. to room temperature.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 2
Compound 3 can be obtained by subjecting compound 2 to cyclization in a solvent in the presence of a base, as necessary in the presence of a catalytic amount of sodium iodide, potassium iodide and the like.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include acetone and N,N-dimethylformamide.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; alkali metal carboxylates such as sodium acetate, potassium acetate and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal alkoxides such as sodium ethoxide, sodium methoxide, potassium tert-butoxide and the like; alkyllithiums such as n-butyllithium, s-butyllithium and the like; alkali metal amides such as lithium diisopropylamide, sodium amide, lithium bistrimethylsilylamide and the like; and the like can be mentioned, with preference given to potassium carbonate.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 100° C.
The reaction time is about 10 min to 48 hr, preferably about 1 hr to 24 hr.
Step 3
Compound 4 can be obtained by reducing compound 3 with a reducing agent in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; and the like can be mentioned, with preference given to toluene, diethyl ether and tetrahydrofuran.
As the reducing agent to be used for the reaction, for example, lithium aluminum hydride, sodium borohydride, diborane, diisobutylaluminum hydride, borane-tetrahydrofuran complex, sodium bis(2-methoxyethoxy)aluminum hydride and the like can be mentioned, with preference given to sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride and borane-tetrahydrofuran complex.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 130° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 4
Compound 5 can be obtained by converting carboxylic acid compound C to the acid chloride with oxalyl chloride, thionyl chloride and the like in a solvent, and amidating compound 4 with the acid chloride in a solvent, as necessary in the presence of a base.
As the solvent to be used for the reaction to obtain the acid chloride, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include 1,2-dimethoxyethane, ethyl acetate, and methylene chloride, chloroform, toluene, 1,2-dimethoxyethane and ethyl acetate each containing a catalytic amount of N,N-dimethylformamide.
The reaction temperature is about −20° C. to 120° C., preferably about 0° C. to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate, N,N-dimethylformamide and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. This reaction is preferably carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide, sodium hydride or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Compound 5 can also be obtained by subjecting compound 4 and carboxylic acid compound C to condensation with, for example, water-soluble carbodiimide (WSC HCl: 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride), dicyclohexylcarbodiimide (DCC), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI), 1-hydroxy-1H-benzotriazole (HOBT), 4-dimethylaminopyridine (DMAP) and the like; or by converting carboxylic acid compound C to a mixed acid anhydride with a chloroformate such as ethyl chloroformate, isopropyl chloroformate, isobutyl chloroformate and the like in the presence of a base such as triethylamine and the like, and reacting compound 4 with the mixed acid anhydride in the presence of a base.
As regards a compound wherein X4 is other than —CH2—, the corresponding compound can be obtained by a method similar to Step 4 and using known compounds.
Step 1 and Step 2 can be conducted in a single step according to Synthesis, 10, 851-852 (1984) to give compound 3.
Production Method 2
A compound [1] wherein X2 is an oxygen atom and Y is C═O can be produced by the following Steps:
wherein each symbol is as defined above.
Step 5
Compound 6 can be obtained by subjecting compound 1 to silylation with tert-butylchlorodimethylsilane in a solvent in the presence of a base.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include N,N-dimethylformamide.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, imidazole, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. The preferable base for this reaction is imidazole.
The reaction temperature is about 0° C. to 150° C., preferably about 0° C. to room temperature.
The reaction time is about 10 min to 24 hr, preferably about 30 min to 12 hr.
Step 6
Compound 7 can be obtained by converting carboxylic acid compound C to the acid chloride with oxalyl chloride, thionyl chloride and the like in a solvent, and amidating compound 6 with the acid chloride in a solvent, as necessary in the presence of a base.
As the solvent to be used for the reaction to obtain the acid chloride, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include 1,2-dimethoxyethane, ethyl acetate, and methylene chloride, chloroform, toluene, 1,2-dimethoxyethane and ethyl acetate each containing a catalytic amount of N,N-dimethylformamide.
The reaction temperature is about −20° C. to 120° C., preferably about 0° C. to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. This reaction is preferably carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Compound 7 can also be obtained by subjecting compound 6 and carboxylic acid compound C to condensation with, for example, water-soluble carbodiimide (WSC HCl: 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride), dicyclohexylcarbodiimide (DCC), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI), 1-hydroxy-1H-benzotriazole (HOBT), 4-dimethylaminopyridine (DMAP) and the like; or by converting carboxylic acid compound C to a mixed acid anhydride with a chloroformate such as ethyl chloroformate, isopropyl chloroformate, isobutyl chloroformate and the like in the presence of a base such as triethylamine and the like, and reacting compound 6 with the mixed acid anhydride in the presence of a base.
Step 7
Compound 8 can be obtained by subjecting compound 7 to desilylation in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include N,N-dimethylformamide.
As the reagent to be used for the reaction, for example, potassium carbonate, tetrabutylammonium fluoride and the like can be mentioned, with preference given to potassium carbonate.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 8
Compound 9 can be obtained by subjecting compound 8 to cyclization with halide D in a solvent in the presence of a base.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include N,N-dimethylformamide.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. The preferable base for this reaction is potassium carbonate.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 100° C.
The reaction time is about 10 min to 48 hr, preferably about 3 hr to 24 hr.
Production Method 3
A compound [1] wherein X2 is a carbon atom or a sulfur atom and Y is C═O can be produced by the following Steps:
wherein each symbol is as defined above.
Step 9
Compound 11 can be obtained by reducing known compound 10 with a reducing agent in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; and the like can be mentioned, with preference given to toluene, diethyl ether and tetrahydrofuran.
As the reducing agent to be used for the reaction, for example, lithium aluminum hydride, sodium borohydride, diborane, diisobutylaluminum hydride, borane-tetrahydrofuran complex, sodium bis(2-methoxyethoxy)aluminum hydride and the like can be mentioned, with preference given to sodium bis(2-methoxyethoxy)aluminum hydride, lithium aluminum hydride and borane-tetrahydrofuran complex.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 130° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 10
Compound 12 can be obtained by converting carboxylic acid compound C to the acid chloride with oxalyl chloride, thionyl chloride and the like in a solvent, and amidating compound 11 with the acid chloride in a solvent, as necessary in the presence of a base.
As the solvent to be used for the reaction to obtain the acid chloride, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include 1,2-dimethoxyethane, ethyl acetate, and methylene chloride, chloroform, toluene, 1,2-dimethoxyethane and ethyl acetate each containing a catalytic amount of N,N-dimethylformamide.
The reaction temperature is about −20° C. to 120° C., preferably about 0° C. to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like, polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate, N,N-dimethylformamide and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. This reaction is preferably carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide, sodium hydride or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
The compound 12 can also be obtained by subjecting compound 11 and carboxylic acid compound C to condensation with, for example, water-soluble carbodiimide (WSC HCl: 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride), dicyclohexylcarbodiimide (DCC), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI), 1-hydroxy-1H-benzotriazole (HOBT), 4-dimethylaminopyridine (DMAP) and the like; or by converting carboxylic acid compound C to a mixed acid anhydride with a chloroformate such as ethyl chloroformate, isopropyl chloroformate, isobutyl chloroformate and the like in the presence of a base such as triethylamine and the like, and reacting compound 11 with the mixed acid anhydride in the presence of a base.
As regards a compound wherein X4 is other than —CH2—, the corresponding compound can be obtained by a method similar to Step 10 and using known compounds.
Production Method 4
A compound [1] wherein X2 is S═O or S(═O)2 and Y is C═O can be produced by the following Step:
wherein each symbol is as defined above.
Step 11
Compound 14 can be obtained by oxidizing compound 13 with an oxidant in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethyl sulfoxide, water, acetic acid and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride and chloroform.
As the oxidant to be used for the reaction, for example, 3-chloroperbenzoic acid, hydrogen peroxide, sodium periodate, tert-butylhydroperoxide and the like can be mentioned, with preference given to 3-chloroperbenzoic acid.
The reaction temperature is about 0° C. to 80° C., preferably about 0° C. to room temperature.
The reaction time is about 10 min to 48 hr, preferably about 2 hr to 24 hr.
Production Method 5
A compound [1] wherein X2 is NR5, N(COR6), N(S(═O)2R6) or N(CONR7R8) and Y is C═O can be produced by the following Step:
wherein each symbol is as defined above.
Step 12
Compound 16 can be obtained by converting carboxylic acid compound C to the acid chloride with oxalyl chloride, thionyl chloride and the like in a solvent, and amidating known compound 15 with the acid chloride in a solvent, as necessary in the presence of a base.
As the solvent to be used for the reaction to obtain the acid chloride, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include 1,2-dimethoxyethane, ethyl acetate, and methylene chloride, chloroform, toluene, 1,2-dimethoxyethane and ethyl acetate each containing a catalytic amount of N,N-dimethylformamide.
The reaction temperature is about −20° C. to 120° C., preferably about 0° C. to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate, N,N-dimethylformamide and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. This reaction is preferably carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide, sodium hydride or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Compound 16 can also be obtained by subjecting known compound 15 and carboxylic acid compound C to condensation with, for example, water-soluble carbodiimide (WSC HC1: 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride), dicyclohexylcarbodiimide (DCC), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI), 1-hydroxy-1H-benzotriazole (HOBT), 4-dimethylaminopyridine (DMAP) and the like; or by converting carboxylic acid compound C to a mixed acid anhydride with a chloroformate such as ethyl chloroformate, isopropyl chloroformate, isobutyl chloroformate and the like in the presence of a base such as triethylamine and the like, and reacting known compound 15 with the mixed acid anhydride in the presence of a base.
Note that R5, COR6, S(═O)2R6 and CONR7R8 can be introduced into the nitrogen atom of X2 before and after Step 12, and the reactions can be carried out in a reasonable order.
Production Method 6
A compound [1] wherein Y is C═S can be produced by the following Step:
wherein each symbol is as defined above.
Step 13
Compound 18 can be obtained by reacting compound 17 with Lawesson reagent, diphosphorus pentasulfide and the like in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include tetrahydrofuran and toluene.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 120° C.
The reaction time is about 10 min to 24 hr, preferably about 30 min to 12 hr.
Production Method 7
A compound [1] wherein Y is S(═O)2 can be produced by the following Step:
wherein each symbol is as defined above.
Step 14
Compound 20 can be obtained by amidating compound 19 with known acid chloride E in a solvent, as necessary in the presence of a base.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate, N,N-dimethylformamide and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. Preferably, this reaction is carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide, sodium hydride or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Production Method 8
A compound [1] wherein X2 is an oxygen atom and Y is C═O can be produced by the following Steps:
wherein TBDMS is a tert-butyldimethylsilyl group, —OMs is—OS(═O)2—CH3, and the other symbols are as defined above.
Step 15
Compound 22 can be obtained by reacting compound 21 with halide F in a solvent in the presence of a base, as necessary in the presence of sodium iodide, potassium iodide and the like.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include acetone and N,N-dimethylformamide.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; alkali metal carboxylates such as sodium acetate, potassium acetate and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal alkoxides such as sodium ethoxide, sodium methoxide, potassium tert-butoxide and the like; alkyllithiums such as n-butyllithium, s-butyllithium and the like; alkali metal amides such as lithium diisopropylamide, sodium amide, lithium bistrimethylsilylamide and the like; and the like can be mentioned, with preference given to sodium hydride and potassium carbonate.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 130° C.
The reaction time is about 10 min to 48 hr, preferably about 1 hr to 24 hr.
Step 16
Compound 23 can be obtained by reducing compound 22 in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include tetrahydrofuran, ethyl acetate and ethanol.
As the reduction reaction, for example, hydrogenation using a noble metal catalyst (e.g., palladium carbon, palladium-barium sulfate, palladium black, platinum carbon, platinum oxide, rhodium carbon, Raney-nickel etc.) and the like, or reduction reaction using tin dichloride, iron, sodium hydrosulfite and the like, and the like can be mentioned, with preference given to hydrogenation using a noble metal catalyst (palladium carbon).
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 100° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 17
Compound 24 can be obtained by converting carboxylic acid compound C to the acid chloride with oxalyl chloride, thionyl chloride and the like in a solvent, and amidating compound 23 with the acid chloride in a solvent, as necessary in the presence of a base.
As the solvent to be used for the reaction to obtain the acid chloride, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include 1,2-dimethoxyethane, ethyl acetate, and methylene chloride, chloroform, toluene, 1,2-dimethoxyethane and ethyl acetate each containing a catalytic amount of N,N-dimethylformamide.
The reaction temperature is about −20° C. to 120° C., preferably about 0° C. to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
As the solvent to be used for the amidation reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include methylene chloride, chloroform, toluene, ethyl acetate and tetrahydrofuran.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; and the like can be mentioned. This reaction is preferably carried out without a base, or in the presence of triethylamine, pyridine, sodium hydroxide or sodium hydrogencarbonate.
The reaction temperature is about 0° C. to 120° C., preferably about 0° C. to 95° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
The compound 24 can also be obtained by subjecting compound 23 and carboxylic acid compound C to condensation with, for example, water-soluble carbodiimide (WSC HCl: 1-ethyl-3-(3′-dimethylaminopropyl)carbodiimide hydrochloride), dicyclohexylcarbodiimide (DCC), diphenylphosphoryl azide (DPPA), carbonyldiimidazole (CDI), 1-hydroxy-1H-benzotriazole (HOBT), 4-dimethylaminopyridine (DMAP) and the like; or by converting carboxylic acid compound C to a mixed acid anhydride with a chloroformate such as ethyl chloroformate, isopropyl chloroformate, isobutyl chloroformate and the like in the presence of a base such as triethylamine and the like, and reacting compound 23 with the mixed acid anhydride in the presence of a base.
Step 18
Compound 25 can be obtained by subjecting compound 24 to desilylation in a solvent.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; alcohols such as methanol, ethanol, isopropyl alcohol, tert-butanol and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include tetrahydrofuran and N,N-dimethylformamide.
As the reagent to be used for the reaction, for example, potassium carbonate, tetrabutylammonium fluoride and the like can be mentioned, with preference given to tetrabutylammonium fluoride.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 80° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 19
Compound 25 can be converted to compound 26.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. Preferably, this reaction is carried out without a solvent or in the presence of methylene chloride or chloroform.
As the reagent to be used for the reaction, for example, carbon tetrachloride in the presence of triphenylphosphine; N-chlorosuccinimide (NCS) in the presence of triphenylphosphine; thionyl chloride; carbon tetrabromide in the presence of triphenylphosphine; N-bromosuccinimide (NBS) in the presence of triphenylphosphine; phosphorus tribromide; phosphorus bromide; iodine in the presence of triphenylphosphine and imidazole; methanesulfonyl chloride in the presence of a base (pyridine, triethylamine etc.); and the like can be mentioned, with preference given to methanesulfonyl chloride in the presence of a base (pyridine, triethylamine etc.).
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 100° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
Step 20
Compound 27 can be obtained by subjecting compound 26 to cyclization in a solvent in the presence of a base, as necessary in the presence of sodium iodide, potassium iodide and the like.
As the solvent to be used for the reaction, for example, ethers such as diethyl ether, tetrahydrofuran, dioxane, 1,2-dimethoxyethane, diglyme and the like; hydrocarbons such as benzene, toluene, hexane, xylene and the like; halogenated hydrocarbons such as methylene chloride, chloroform, carbon tetrachloride, 1,2-dichloroethane and the like; esters such as ethyl acetate, methyl acetate, butyl acetate and the like; polar solvents such as acetone, N,N-dimethylformamide, dimethylsulfoxide and the like; and the like can be mentioned. These can be used alone or in a mixture of two or more kinds thereof. The preferable solvents for this reaction include tetrahydrofuran and N,N-dimethylformamide.
As the base to be used for the reaction, for example, organic bases such as triethylamine, pyridine, 4-dimethylaminopyridine, N-methylmorpholine and the like; alkali metal hydroxides such as lithium hydroxide, sodium hydroxide, potassium hydroxide and the like; alkali metal carbonates such as sodium carbonate, potassium carbonate and the like; alkali metal hydrogencarbonates such as sodium hydrogencarbonate, potassium hydrogencarbonate and the like; alkali metal carboxylates such as sodium acetate, potassium acetate and the like; alkali metal hydrides such as sodium hydride, potassium hydride and the like; alkali metal alkoxides such as sodium ethoxide, sodium methoxide, potassium tert-butoxide and the like; alkyllithiums such as n-butyllithium, s-butyllithium and the like; alkali metal amides such as lithium diisopropylamide, sodium amide, lithium bistrimethylsilylamide and the like; and the like can be mentioned, with preference given to potassium carbonate and sodium hydride.
The reaction temperature is about 0° C. to 150° C., preferably room temperature to 100° C.
The reaction time is about 10 min to 48 hr, preferably about 30 min to 24 hr.
The crystal of the present invention can also be produced by applying a crystallization method known per se to the compound of the present invention.
Here, as the crystallization method, for example, crystallization from a solution, crystallization from vapor, crystallization from a molten form and the like can be mentioned.
For the “crystallization from a solution”, a method comprising shifting from a non-saturation state to a supersaturation state by changing the factors (solvent composition, pH, temperature, ionic strength, oxidization-reduction state etc.) relating to the solubility of the compound or the amount of solvent is generally employed. Specifically, for example, concentration method, slow cooling method, reaction methods (diffusion method, electrolysis method), hydrothermal growth method, fusing agent method and the like can be mentioned. As the solvent to be used, for example, aromatic hydrocarbons (e.g., benzene, toluene, xylene etc.), halogenated hydrocarbons (e.g., dichloromethane, chloroform etc.), saturated hydrocarbons (e.g., hexane, heptane, cyclohexane etc.), ethers (e.g., diethyl ether, diisopropyl ether, tetrahydrofuran, dioxane etc.), nitrites (e.g., acetonitrile etc.), ketones (e.g., acetone etc.), sulfoxides (e.g., dimethyl sulfoxide etc.), acid amides (e.g., N,N-dimethylformamide etc.), esters (e.g., ethyl acetate etc.), alcohols (e.g., methanol, ethanol, isopropyl alcohol, n-butanol etc.), water and the like can be mentioned. These solvents are used alone or a mixture of two or more thereof at a suitable ratio (e.g., 1:1 to 1:100 (volume ratio)).
For the “crystallization from vapor”, for example, gasification methods (sealed tube method, gas stream method), gas phase reaction method, chemical transportation method and the like can be mentioned.
For the “crystallization from a molten form”, for example, normal freezing methods (pulling-up method, temperature gradient method, Bridgman method), zone melting methods (zone leveling method, float zone method), special growth methods (VLS method, liquid phase epitaxis method) and the like can be mentioned.
As preferable examples of crystallization, a method wherein the compound of the present invention is dissolved in a suitable solvent (e.g., alcohols such as methanol, ethanol etc. and the like) at a temperature of 20-120° C., the obtained solution is cooled to a temperature not more than that for dissolution (e.g., 0-50° C., preferably 0-20° C.) and the like can be mentioned.
The crystal of the present invention thus obtained can be isolated by, for example, filtration and the like.
The production of the compound of the present invention is concretely explained by referring to Examples, which are not to be construed as limitative.
In the Examples, the room temperature means 1-40° C.
Step 1
Chloroform (20 mL) was added to 3-chloro-4-methoxybenzoic acid (2.0 g), and oxalyl chloride (1.84 mL) and N,N-dimethylformamide (1 drop) were added under ice-cooling. The mixture was stirred at room temperature for 3 hrs, concentrated and azeotroped with toluene to give the title compound (2.063 g).
Step 2
Synthesis was performed in reference to Australian journal of chemistry, 9, 397-405 (1956). To be specific, lithium aluminum hydride (3 g) was suspended in tetrahydrofuran (120 mL), and 2H-1,4-benzoxazin-3(4H)-one (6 g) was added by small portions under ice-cooling. After heating under reflux for 10 hrs, water (3 mL), 15% aqueous sodium.hydroxide (3 mL) and water (9 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (4.9239 g) as an orange oil.
Step 3
3,4-Dihydro-2H-benzo[1,4]oxazine (525 mg) was dissolved in chloroform (10 mL), and triethylamine (0.65 mL) and 3-chloro-4-methoxybenzoyl chloride (836 mg) were added under ice-cooling. The mixture was stirred at room temperature for 12 hrs, and the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (1.180 g) as a white solid.
Step 4
(3-Chloro-4-methoxyphenyl)- (2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (1.175 g) was dissolved in chloroform (10 mL). Boron tribromide (1.0 M methylene chloride solution, 7.74 mL) was added dropwise at −45° C., and the mixture was stirred at room temperature for 3 hrs. Water and ethyl acetate were added to the reaction mixture, and the mixture was extracted with chloroform. The obtained chloroform layer was washed with water and saturated aqueous sodium hydrogencarbonate, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the residue was purified by silica gel chromatography (n-hexane-ethyl acetate=65:35) to give the title compound (124 mg) as white crystals.
Step 1
1,2-Dimethoxyethane (30 mL) was added to 3-bromo-4-hydroxybenzoic acid (3.25 g) to dissolve same by heating the mixture to 80° C. Thionyl chloride (1.6 mL) was added, and the mixture was stirred overnight at 80° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound (3.6181 g) as a white solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (203 mg) obtained in Step 2 of Example 1 and 3-bromo-4-hydroxybenzoyl chloride (353 mg) were dissolved in ethyl acetate (4 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (236.7 mg) as beige crystals.
Step 1
1,2-Dimethoxyethane (30 mL) was added to 3,5-dichloro-4-hydroxybenzoic acid (1.242 g) to dissolve same by heating the mixture to 80° C. Thionyl chloride (0.57 mL) was added, and the mixture was stirred overnight at 80° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound (1.358 g) as a white solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (135 mg) obtained in Step 2 of Example 1 and 3,5-dichloro-4-hydroxybenzoyl chloride (225 mg) were dissolved in ethyl acetate (3.2 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (282 mg) as white crystals.
Step 1
1,2-Dimethoxyethane (20 mL) was added to 3,5-dibromo-4-hydroxybenzoic acid (2.96 g) to dissolve same by heating the mixture to 80° C. Thionyl chloride (1.1 mL) was added, and the mixture was stirred overnight at 80° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound (3.1562 g) as a white solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1 and 3,5-dibromo-4-hydroxybenzoyl chloride (629 mg) were dissolved in ethyl acetate (10 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (700.9 mg) as pale-orange crystals.
Step 1
1,2-Dimethoxyethane (12 mL) was added to 4-hydroxy-3,5-diiodobenzoic acid (2.34 g) to dissolve same by heating the mixture to 80° C. Thionyl chloride (0.66 mL) was added, and the mixture was stirred overnight at 80° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound (2.4922 g) as a cream color solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (406 mg) obtained in Step 2 of Example 1 and 4-hydroxy-3,5-diiodobenzoyl chloride (1.26 g) were dissolved in ethyl acetate (15 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (1.2887 g) as pale-orange crystals.
Step 1
Chloroform (20 mL) was added to 3,5-difluoro-4-methoxybenzoic acid (2 g), and oxalyl chloride (1.87 mL) and N,N-dimethylformamide (1 drop) were added under ice-cooling. After stirring at room temperature for 3 hrs, the reaction mixture was concentrated under reduced pressure, and azeotroped with toluene to give the title compound as an oil.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (300 mg) obtained in Step 2 of Example 1 was dissolved in chloroform (6 mL) and triethylamine(0.371 mL) and 3,5-difluoro-4-methoxybenzoyl chloride (459 mg) were added under ice-cooling. The mixture was stirred at room temperature for 12 hrs, and the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (709 mg) as an oil.
Step 3
(3,5-Difluoro-4-methoxyphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (672 mg) was dissolved in methylene chloride (7 mL). After cooling to −78° C., boron tribromide (1.0 M methylene chloride solution, 3.3 mL) was added dropwise, and the mixture was stirred at room temperature for 20 hrs. The reaction mixture was poured into water, and the mixture was extracted with chloroform. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=7:3) to give the title compound (258 mg) as white crystals.
Step 1
Methylene chloride (8 mL) was added to 4-benzyloxy-3,5-dimethylbenzoic acid (256 mg), and oxalyl chloride (0.1 mL) and N,N-dimethylformamide (1 drop) were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was concentrated under reduced pressure, and azeotroped with toluene to give the title compound (276.7 mg) as a pale-yellow solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (135 mg) obtained in Step 2 of Example 1 was dissolved in methylene chloride (7 mL), and triethylamine (0.17 mL) and 4-benzyloxy-3,5-dimethylbenzoyl chloride (275 mg) were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (378.3 mg) as an oil.
Step 3
(4-Benzyloxy-3,5-dimethylphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (370.1 mg) was dissolved in tetrahydrofuran (10 mL). 7.5% Palladium-carbon (37 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (220.1 mg) as white crystals.
Step 1
Ethyl 3,5-dichloro-4-hydroxybenzoate (55.64 g) was dissolved in N,N-dimethylformamide (280 mL) and potassium carbonate (42.56 g) was added. Under ice-cooling, benzyl bromide (36 mL) was added dropwise, and the mixture was stirred overnight at 70° C. The solvent was evaporated, and the mixture was partitioned between water and ethyl acetate. The ethyl acetate layer was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was recrystallized from n-hexane to give the title compound (32.45 g). In addition, the filtrate was concentrated to give the title compound (42.19 g).
Step 2
Ethyl 4-benzyloxy-3,5-dichlorobenzoate (42.19 g) was dissolved in methanol (70 mL) and tetrahydrofuran (140 mL). Under ice-cooling, 2N aqueous lithium hydroxide (130 mL) was added dropwise, and the mixture was stirred overnight at room temperature. A small amount of an insoluble material was removed by filtration. The filtrate was concentrated. Water was added, and the mixture was acidified by dropwise addition of 1N hydrochloric acid under ice-cooling. The precipitated solid was collected by filtration to give the title compound (34.83 g).
Step 3
Chloroform (175 mL) was added to 4-benzyloxy-3,5-dichlorobenzoic acid (34.83 g), and oxalyl chloride (15.3 mL) and N,N-dimethylformamide (2 drops) were added under ice-cooling. The mixture was stirred at room temperature for 4 hrs, an insoluble material was removed by filtration, the filtrate was concentrated and azeotroped with toluene to give the title compound (37.371 g) as a pale-yellow solid.
Step 4
Lithium aluminum hydride (1 g) was suspended in tetrahydrofuran (40 mL), 4H-benzo[1,4]thiazin-3-one (2 g) was added under ice-cooling by small portions. The mixture was heated under reflux for 8 hrs, and water (1 mL), 15% aqueous sodium hydroxide (1 mL) and water (3 mL) were successively added lo under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate, and concentrated to give the title compound (1.9181 g) as a yellow oil.
Step 5
3,4-Dihydro-2H-benzo[1,4]thiazine (1 g) was dissolved in chloroform (19 mL), and triethylamine (1.1 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (2.08 g) were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (2.3350 g) as an oil.
Step 6
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]thiazin-4-yl)-methanone (223.7 mg) was dissolved in toluene (2 mL), and trifluoroacetic acid (2 mL) was added at room temperature. After stirring with heating at 80° C. for 1.5 hrs, the mixture was concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (101.6 mg) as white crystals.
Step 1
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]thiazin-4-yl)-methanone (426 mg) obtained in Step 5 of Example 8 was dissolved in chloroform (10 mL), 3-chloroperbenzoic acid (171 mg) was added under ice-cooling, and the mixture was stirred overnight at room temperature. Saturated aqueous sodium hydrogencarbonate was added to the reaction solution, and the mixture was extracted with chloroform. The extract was dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:3) to give the title compound (398.4 mg) as a white amorphous form.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(1-oxo-2,3-dihydro-1H-1λ4-benzo[1,4]thiazin-4-yl)-methanone (210.9 mg) was dissolved in toluene (2 mL), and trifluoroacetic acid (2 mL) was added at room temperature. After stirring with heating at 80° C. for 1.5 hrs, the mixture was concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (157.5 mg) as pale-blue crystals.
Step 1
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]thiazin-4-yl)-methanone (496.6 mg) obtained in Step 5 of Example 8 was dissolved in chloroform (15 mL), 3-chloroperbenzoic acid (597 mg) was added under ice-cooling, and the mixture was stirred overnight at room temperature. The insoluble material was removed by filtration, saturated aqueous sodium hydrogencarbonate was added to the filtrate, and the mixture was extracted with chloroform. The extract was dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=2:1) to give the title compound (363.9 mg) as a white solid.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(1,1-dioxo-2,3-dihydro-1H-1λ6-benzo[1,4]thiazin-4-yl)-methanone (245 mg) was dissolved in toluene (2.5 mL), and trifluoroacetic acid (2.5 mL) was added at room temperature. After stirring with heating at 80° C. for 2 hrs, and the mixture was concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (112 mg) as white crystals.
Step 1
3,4-Dihydro-2H-benzo[1,4]oxazine (0.4 g) obtained in Step 2 of Example 1 was dissolved in chloroform (20 mL), triethylamine (0.5 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (0.947 g) obtained in Step 3 of Example 8 were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (1.0635 g) as a white solid.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (400 mg) and Lawesson reagent (235 mg) were suspended in tetrahydrofuran (3 mL), and the mixture was stirred with heating at 95° C. for 2 hrs. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (372.4 mg) as an orange amorphous form.
Step 3
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanethione (366.8 mg) was dissolved in toluene (4 mL), and trifluoroacetic acid (4 mL) was added at room temperature. After stirring with heating at 80° C. for 2 hrs, and the mixture was concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (84.6 mg) as orange crystals.
Step 1
Lithium aluminum hydride (0.8 g) was suspended in tetrahydrofuran (50 mL), and 6-methyl-4H-benzo[1,4]oxazin-3-one (1.76 g) was added under ice-cooling by small portions. After heating under reflux for 6 hrs, water (0.8 mL), 15% aqueous sodium hydroxide (0.8 mL) and water (2.4 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (1.5719 g) as an orange oil.
Step 2
6-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (298 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (451 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (7 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (606.4 mg) as pale-orange crystals.
Step 1
2-Amino-5-methylphenol (2.463 g) and benzyltriethylammonium chloride (4.56 g) were suspended in chloroform (50 mL), sodium hydrogencarbonate (13.44 g) and chloroacetyl chloride (1.9 mL) were added under ice-cooling and the mixture was stirred under ice-cooling for 1 hr. Thereafter, and the mixture was stirred overnight with heating at 55° C. The reaction mixture was concentrated, water was added and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (1.7636 g) as a khaki solid.
Step 2
Lithium aluminum hydride (0.8 g) was suspended in tetrahydrofuran (50 mL), and 7-methyl-4H-benzo[1,4]oxazin-3-one (1.76 g) was added under ice-cooling by small portions. After heating under reflux for 6 hrs, water (0.8 mL), 15% aqueous sodium hydroxide (0.8 mL) and water (2.4 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (1.5038 g) as an orange-tan oil.
Step 3
7-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (298 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (451 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (7 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (609.3 mg) as a white amorphous solid.
Step 1
2-Amino-3-methylphenol (737 mg) was dissolved in tetrahydrofuran (20 mL), triethylamine (1 mL) and chloroacetyl chloride (0.5 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.2217 g) as a khaki solid.
Step 2
2-chloro-N-(2-hydroxy-6-methylphenyl)-acetamide (1.2217 g) was dissolved in N,N-dimethylformamide (7 mL), and potassium carbonate (0.99 g) and sodium iodide (catalytic amount) were added at room temperature. After stirring overnight at 80° C., the mixture was poured into water. The mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid and water, then successively washed with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (813.5 mg) as a pale-yellow solid.
Step 3
Lithium aluminum hydride (0.4 g) was suspended in tetrahydrofuran (40 mL) and, under ice-cooling, 5-methyl-4H-benzo[1,4]oxazin-3-one (810 mg) was added by small portions. After heating under reflux for 7 hrs, water (0.4 mL), 15% aqueous sodium hydroxide (0.4 mL) and water (1.2 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (471.7 mg) as a red brown oil.
Step 4
5-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (224 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (338 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3.5 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (331.9 mg) as gray crystals.
Step 1
o-Cresol (5 g) was dissolved in acetic acid (50 mL) and 48% aqueous hydrogen bromide (25 mL), dimethyl sulfoxide (25 mL) was added dropwise at room temperature. After stirring at room temperature for 1 hr, the reaction mixture was neutralized with sodium carbonate. Water was added, and the mixture was extracted with ethyl ether. The obtained ethyl ether layer was washed successively with water and saturated brine, and dried over magnesium sulfate. The solvent was evaporated, and the residue was used for the next Step without purification.
Step 2
To a mixture of 4-bromo-2-methylphenol produced in Step 1 and sodium nitrite (10.4 g) were added n-hexane (75 mL), isopropyl ether (35 mL) and water (50 mL), and 4.5N sulfuric acid (110 mL) was added dropwise at room temperature. After stirring at room temperature for 15 hrs, the reaction mixture was washed successively with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (7.14 g) as a yellow solid.
Step 3
4-Bromo-2-methyl-6-nitrophenol (7.1 g) was dissolved in methanol (50 mL). 7.5% Palladium-carbon (1.5 g) was added to this solution, and under a hydrogen atmosphere (2 kgf/cm2), and the mixture was stirred at room temperature for 18 hrs. The reaction mixture was filtered through celite, and the filtrate was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (5.40 g) as a brown solid.
Step 4
2-Amino-6-methylphenol hydrobromide (408 mg) was suspended in tetrahydrofuran (10 mL), triethylamine (0.7 mL) and lo chloroacetyl chloride (0.17 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (384.6 mg) as a dark brown solid.
Step 5
2-Chloro-N-(2-hydroxy-3-methylphenyl)-acetamide (380 mg) was dissolved in N,N-dimethylformamide (4 mL), potassium carbonate (315 mg) and sodium iodide (catalytic amount) were added at room temperature. After stirring overnight at 80° C., the mixture was poured into water and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer washed successively with 1N hydrochloric acid and water, and successively with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (284.3 mg) as a red brown solid.
Step 6
Lithium aluminum hydride (150 mg) was suspended in tetrahydrofuran (25 mL), 8-methyl-4H-benzo[1,4]oxazin-3-one (280 mg) was added under ice-cooling by small portions. After heating under reflux for 7 hrs, water (0.15 mL), 15% aqueous sodium hydroxide (0.15 mL) and water (0.45 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (145.2 mg) as a red brown oil.
Step 7
8-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (116.3 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (176 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (2 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (121.3 mg) as pale-brown crystals.
Step 1
1-Aminonaphthalen-2-ol hydrochloride (1.1739 g) was suspended in tetrahydrofuran (20 mL), triethylamine (2 mL) and chloroacetyl chloride (0.5 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.227 g) as a dark brown solid.
Step 2
2-Chloro-N-(2-hydroxynaphthalen-1-yl)-acetamide (1.22 g) was dissolved in N,N-dimethylformamide (10 mL), and potassium carbonate (860 mg) and sodium iodide (catalytic amount) were added at room temperature. After stirring overnight at 80° C., and the mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid and water, subsequently with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.0553 g) as a dark brown solid.
Step 3
Lithium aluminum hydride (400 mg) was suspended in tetrahydrofuran (40 mL), 1H-naphtho[2,1-b][1,4]oxazin-2-one (1.055 g) was added under ice-cooling by small portions. After heating under reflux for 7 hrs, water (0.4 mL), 15% aqueous sodium hydroxide (0.4 mL) and water (1.2 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (361.4 mg) as a dark brown oil.
Step 4
2,3-Dihydro-1H-naphtho[2,1-b][1,4]oxazine (185 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (225 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (334.5 mg) as gray crystals.
Step 1
4-Methoxy-2-nitrophenol (3.38 g) was dissolved in tetrahydrofuran (100 mL). 7.5% Palladium-carbon (0.34 g) was added to this solution and, under a hydrogen atmosphere, and the mixture was stirred at room temperature for 3.5 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentration under reduced pressure to give the title compound (2.8528 g) as a beige solid.
Step 2
2-Amino-4-methoxyphenol (2.85 g) and benzyltriethylammonium chloride (4.56 g) were suspended in chloroform (50 mL), sodium hydrogencarbonate (6.72 g) and chloroacetyl chloride (1.9 mL) were added under ice-cooling, and the mixture was stirred under ice-cooling for 1 hr. Thereafter, the mixture was stirred overnight with heating at 55° C. The reaction mixture was concentrated, water was added and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (1.3884 g) as a pale-orange solid. The mother liquor was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (151 mg) as a white solid.
Step 3
Lithium aluminum hydride (700 mg) was suspended in tetrahydrofuran (50 mL), 6-methoxy-4H-benzo[1,4]oxazin-3-one (1.53 g) was added under ice-cooling by small portions. After heating under reflux for 6 hrs, water (0.7 mL), 15% aqueous sodium hydroxide (0.7 mL) and water (2.1 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (1.3294 g) as a pale-yellow solid.
Step 4
6-Methoxy-3,4-dihydro-2H-benzo[1,4]oxazine (600 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (818 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (14 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (1.059 g) as white crystals.
Step 1
5-Methoxy-2-nitrophenol (1.059 g) was dissolved in tetrahydrofuran (30 mL). 7.5% Palladium-carbon (0.1 g) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 3 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure to give the title compound (0.9615 g) as a pale-orange solid.
Step 2
2-Amino-5-methoxyphenol (0.96 g) was dissolved in tetrahydrofuran (35 mL), triethylamine (1.15 mL) and chloroacetyl chloride (0.58 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.5106 g) as a bright yellow solid.
Step 3
2-Chloro-N-(2-hydroxy-4-methoxyphenyl)-acetamide (1.51 g) was dissolved in N,N-dimethylformamide (7 mL), and potassium carbonate (1.04 g) and sodium iodide (catalytic amount) were added at room temperature. After stirring overnight at 80° C., and the mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with 1N hydrochloric acid and water, and sequentially washed with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from ethyl acetate to give the title compound (682.6 mg) as a bright yellow solid.
Step 4
Lithium aluminum hydride (300 mg) was suspended in tetrahydrofuran (20 mL), 7-methoxy-4H-benzo[1,4]oxazin-3-one (680 mg) was added under ice-cooling by small portions. After heating under reflux for 6 hrs, water (0.3 mL), 15% aqueous sodium hydroxide (0.3 mL) and water (0.9 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=2:1) to give the title compound (588.1 mg) as a red brown oil.
Step 5
7-Methoxy-3,4-dihydro-2H-benzo[1,4]oxazine (367.6 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (496 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (4 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (518.2 mg) as light purple crystals.
(3,5-Dichloro-4-hydroxyphenyl)-(6-methoxy-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (500 mg) obtained in Step 4 of Example 17 was dissolved in methylene chloride (10 mL). After cooling to −78° C., boron tribromide (1.0 M methylene chloride solution, 2.1 mL) was added dropwise, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the mixture was extracted with chloroform, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=10:1) to give the title compound (157.6 mg) as pale-blue crystals.
(3,5-Dichloro-4-hydroxyphenyl)-(7-methoxy-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (504 mg) obtained in Step 5 of Example 18 was dissolved in methylene chloride (10 mL). After cooling to −78° C., boron tribromide (1.0 M methylene chloride solution, 4.2 mL) was added dropwise, and the mixture was stirred overnight at room temperature. The reaction mixture was poured into water, and the precipitated solid was collected by filtration. The collected solid was purified by silica gel chromatography (chloroform-methanol=30:1) to give the title compound (355.7 mg) as white crystals.
Step 1
3-Amino-N,N-diethyl-4-methoxybenzenesulfonamide (5 g) was dissolved in methylene chloride (150 mL). Under ice-cooling, boron tribromide (1.0 M methylene chloride solution, 38.7 mL) was added dropwise, and the mixture was stirred overnight at room temperature. Water (150 mL) was added dropwise to the reaction mixture under ice-cooling, and the aqueous layer was washed with chloroform. The obtained aqueous layer was weak acidified with 4N aqueous sodium hydroxide under ice-cooling. The precipitated solid was collected by filtration to give the title compound (4.0344 g) as a pale-beige solid.
Step 2
3-Amino-N,N-diethyl-4-hydroxybenzenesulfonamide (1.5 g) was dissolved in N,N-dimethylformamide (8 mL), and imidazole (0.61 g) and tert-butylchlorodimethylsilane (1.18 g) were added under ice-cooling. After stirring at room temperature for 1.5 hrs, the mixture was poured into water, and the mixture was extracted with ethyl ether. The obtained ethyl ether layer was washed with water and saturated brine, dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=7:1) to give the title compound (2.0654 g) as a white solid.
Step 3
3-Amino-4-(tert-butyldimethylsilyloxy)-N,N-diethylbenzenesulfonamide (1.5 g) was dissolved in methylene chloride (40 mL), pyridine (0.41 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (1.32 g) obtained in Step 3 of Example 8 were added under ice-cooling, and the mixture was stirred overnight at room temperature. The solvent was evaporated and the residue was dissolved in N,N-dimethylformamide (5 mL), and potassium carbonate (2.89 g) was added at room temperature. After stirring with heating at 60° C. for 1.5 hrs, the mixture was acidified with 10% aqueous citric acid solution and water under ice-cooling. The precipitated solid was collected by filtration to give the title compound (2.1455 g) as a cream color solid.
Step 4
4-Benzyloxy-3,5-dichloro-N-(5-diethylsulfamoyl-2-hydroxyphenyl)-benzamide (2.136 g) was dissolved in N,N-dimethylformamide (40 mL), and potassium carbonate (1.41 g) and 1,2-dibromoethane (0.42 mL) were added at room temperature. After stirring overnight at 70° C., the mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=5:2) to give the title compound (1.6181 g) as a white amorphous form.
Step 5
4-(4-Benzyloxy-3,5-dichlorobenzoyl)-3,4-dihydro-2H-benzo[1,4]oxazine-6-sulfonic acid diethylamide (1.6151 g) was dissolved in tetrahydrofuran (70 mL). 7.5% Palladium-carbon (0.16 g) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from ethyl ether to give the title compound (1.2863 g) as white crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (246 mg) obtained in Step 2 of Example 1 and 3,5-dichloro-4-hydroxybenzenesulfonyl chloride (475 mg) were dissolved in chloroform (8 mL), and the mixture was stirred overnight at room temperature. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=2:1) to give the title compound (146.8 mg) as beige crystals.
The title compound (539.6 mg) was obtained as white crystals by a method similar to Example 14 and using 2-amino-4-tert-butylphenol instead of 2-amino-3-methylphenol.
Step 1
4H-Benzo[1,4]oxazin-3-one (224 mg) was dissolved in tetrahydrofuran (10 mL), 60% sodium hydride (78 mg) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (473 mg) obtained in Step 3 of Example 8 were successively added with stirring under ice-cooling, and the mixture was stirred overnight at room temperature. Under ice-cooling, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with saturated aqueous sodium hydrogencarbonate, water and saturated brine, dried over anhydrous sodium sulfate, and the mixture was concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (294.3 mg) as a yellow solid.
Step 2
4-(4-Benzyloxy-3,5-dichlorobenzoyl)-4H-benzo[1,4]oxazin-3-one (290 mg) was dissolved in tetrahydrofuran (12 mL). 7.5% Palladium-carbon (29 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (176.8 mg) as yellow crystals.
The title compound (160.6 mg) was obtained as white crystals by a method similar to Step 2 to 5 of Example 21 and using 3-amino-4-hydroxybenzenesulfonamide instead of 3-amino-N,N-diethyl-4-hydroxybenzenesulfonamide.
Step 1
1,2,3,4-Tetrahydroquinoline (147 mg) was dissolved in chloroform (6 mL), and triethylamine (0.18 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (347 mg) obtained in Step 3 of Example 8 were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (407.9 mg) as a white amorphous form.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(3,4-dihydro-2H-quinolin-1-yl)-methanone (398.7 mg) was dissolved in tetrahydrofuran (12 mL). 7.5% Palladium-carbon (40 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (267.4 mg) as white crystals.
Step 1
Lithium aluminum hydride (100 mg) was suspended in tetrahydrofuran (10 mL), 1,3,4,5-tetrahydrobenzo[b]azepin-2-one (245 mg) was added under ice-cooling by small portions. After heating under reflux for 5.5 hrs, water (0.1 mL), 15% aqueous sodium hydroxide (0.1 mL) and water (0.3 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate, and concentrated to give the title compound (301.6 mg) as a yellow oil.
Step 2
2,3,4,5-Tetrahydro-1H-benzo[b]azepine (301 mg) was dissolved in methylene chloride (10 mL), triethylamine (0.25 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (480 mg) obtained in Step 3 of Example 8 were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (531.9 mg) as a yellow green oil.
Step 3
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3,4,5-tetrahydrobenzo[b]azepin-1-yl)-methanone (530 mg) was dissolved in tetrahydrofuran (15 mL). 7.5% Palladium-carbon (53 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (259.6 mg) as white crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1,4-amino-3,5-dichlorobenzoic acid (412 mg) and N,N-dimethylaminopyridine (269 mg) were dissolved in chloroform (8 mL), and WSC.HCl (422 mg) was added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (412.5 mg) as bright yellow crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1, 5-chloro-6-hydroxynicotinoic acid (347 mg) and 4-dimethylaminopyridine (269 mg) were, dissolved in chloroform (12 mL), and WSC.HCl (422 mg) was added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (chloroform-methanol=20:1) to give the title compound (295.7 mg) as cream color crystals.
Step 1
1,2-Dimethoxyethane (5 mL) was added to 4-hydroxy-3,5-dinitrobenzoic acid (1 g) to dissolve same by heating the mixture to 70° C. Thionyl chloride (0.415 mL) was added, and the mixture was stirred overnight at 70° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound as a yellow solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (135 mg) obtained in Step 2 of Example 1 and 4-hydroxy-3,5-dinitrobenzoyl chloride (271 mg) ware dissolved in ethyl acetate (4 mL), and the mixture was heated under reflux for 3 hrs. The solvent was evaporated, and the obtained solid was crystallized from methanol to give the title compound (211 mg) as pale-yellow crystals.
Step 1
Concentrated sulfuric acid (200 mL) was added to methyl 3-chloro-4-methoxybenzoate (24.6 g) under ice-cooling and a mixture of fuming nitric acid (10.3 mL) and concentrated sulfuric acid (20 mL) was successively added dropwise under ice-cooling. After stirring under ice-cooling, the reaction mixture was poured into ice water (1 L). The precipitated solid was collected by filtration to give the title compound (28.8 g) as a cream color solid.
Step 2
Methyl 3-chloro-4-methoxy-5-nitrobenzoate (28.8 g) was suspended in dimethyl sulfoxide (130 mL), and 50% aqueous potassium hydroxide (130 mL) was added under ice-cooling. After stirring with heating at 80° C. for 1.5 hrs, the mixture was ice-cooled, and 6N hydrochloric acid (200 mL) and water were added. The mixture was extracted with ethyl acetate, and the extract was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from n-hexane to give the title compound (21.3 g) as a lemon solid.
Step 3
1,2-Dimethoxyethane (5 mL) was added to 3-chloro-4-hydroxy-5-nitrobenzoic acid (1 g) to dissolve same by heating the mixture to 70° C. Thionyl chloride (0.436 mL) was added, and the mixture was stirred overnight at 70° C. The reaction mixture was concentrated under reduced pressure, azeotroped with toluene, and dried to give the title compound (1.10 g) as a yellow solid.
Step 4
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1 and 3-chloro-4-hydroxy-5-nitrobenzoyl chloride (519 mg) were dissolved in ethyl acetate (6 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (537 mg) as yellow crystals.
Step 1
2-Isopropylphenol (25 g) was dissolved in acetic acid (250 mL) and 48% aqueous hydrogen bromide (125 mL), and dimethyl sulfoxide (125 mL) was added dropwise at room temperature. After stirring at room temperature for 2 hrs, the reaction mixture was neutralized with sodium carbonate (257 g). Water was added, and the mixture was extracted with ethyl ether. The obtained ethyl ether layer was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (40.2 g) as a pale-yellow solid.
Step 2
To a mixture of 4-bromo-2-isopropylphenol (40.2 g) produced in Step 1 and sodium nitrite (41.5 g) were added n-hexane (300 mL), isopropyl ether (130 mL) and water (200 mL), and 4.5N sulfuric acid (430 mL) was added dropwise at room temperature. After stirring at room temperature for 1 hr, the reaction mixture was washed successively with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=20:1) to give the title compound (41.1 g) as a yellow oil.
Step 3
4-Bromo-2-isopropyl-6-nitrophenol (41.1 g) was dissolved in methanol (300 mL). 7.5% Palladium-carbon (8 g) was added to this solution and, under a hydrogen atmosphere (2 kgf/cm2), the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the filtrate was concentrated under reduced pressure. The obtained solid was crystallized from ethyl acetate to give the title compound (27.4 g) as a beige solid.
Step 4
Chloroform (200 mL) was added to 2-bromo-3-methylbutyric acid (25 g), and oxalyl chloride (14.5 mL) and N,N-dimethylformamide (3 drops) were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was concentrated under reduced pressure, and azeotroped with toluene to give the title compound as a yellow oil.
Step 5
Ethyl acetate (70 mL) and water (80 mL) were added to 2-amino-6-isopropylphenol hydrobromide (6.14 g) obtained in Step 3 and dissolved therein. Sodium hydrogencarbonate (6.68 g) and 2-bromo-3-methylbutyryl chloride (5.80 g) obtained in Step 4 were added at room temperature, and the mixture was stirred at room temperature for 2 hrs. The reaction mixture was partitioned. The obtained ethyl acetate layer was washed successively with saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (9.0 g) as a pale-pink solid.
Step 6
2-Bromo-N-(2-hydroxy-3-isopropylphenyl)-3-methylbutyramide (9.0 g) was dissolved in N,N-dimethylformamide (45 mL), potassium carbonate (4.94 g) was added, and the mixture was stirred overnight at room temperature. The reaction mixture was neutralized with 1N hydrochloric acid, and water was added. The precipitated solid was collected by filtration. The obtained solid was crystallized from n-hexane-isopropyl ether to give the title compound (4.5 g) as a white solid.
Step 7
2,8-Diisopropyl-4H-benzo[1,4]oxazin-3-one (1.0 g) was dissolved in tetrahydrofuran (10 mL), borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 5.14 mL) was added, and the mixture was heated under reflux for 14.5 hrs. 6N Hydrochloric acid (5 mL) was added, and the mixture was stirred with heating again. The mixture was allowed to cool to room temperature, neutralized with sodium hydrogencarbonate, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (768 mg) as a colorless oil.
Step 8
2,8-Diisopropyl-3,4-dihydro-2H-benzo[1,4]oxazine (150 mg) was dissolved in chloroform (5 mL), and pyridine (0.066 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (227 mg) obtained in Step 3 of Example 8 were added under ice-cooling. After stirring overnight at room temperature, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (375 mg) as an oil.
Step 9
(4-Benzyloxy-3,5-dichlorophenyl)-(2,8-diisopropyl-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (370 mg) was dissolved in tetrahydrofuran (5 mL). 7.5% Palladium-carbon (70 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from n-hexane to give the title compound (212 mg) as white crystals.
Step 1
1-Methoxy-2-nitrobenzene (11 mL) was added dropwise to chlorosulfonic acid (25 mL) under ice-cooling, and the mixture was stirred at room temperature for 1.5 hrs. The reaction mixture was poured into ice water and the mixture was extracted with ethyl ether. The obtained ethyl ether layer was washed with water, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (15.1527 g) as a red brown oil.
Step 2
4-Methoxy-3-nitrobenzenesulfonyl chloride (5.1531 g) was dissolved in pyridine (17 mL), pyrrolidine (2.05 g) was added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated under reduced pressure, the residue was poured into water. The mixture was extracted with chloroform, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:1) to give the title compound (4.158 g) as a yellow solid.
Step 3
1-(4-Methoxy-3-nitrobenzenesulfonyl)pyrrolidine (2.054 g) was dissolved in dimethyl sulfoxide (40 mL), and 50% aqueous potassium hydroxide (40 mL) was added under ice-cooling. After stirring with heating at 80° C. for 6 hrs, the mixture was ice-cooled, and 6N hydrochloric acid (80 mL) was added. The mixture was extracted with chloroform. The extract was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (2.198 g) as a yellow solid.
Step 4
2-Nitro-4-(pyrrolidine-1-sulfonyl)phenol (2.19 g) was dissolved in tetrahydrofuran (50 mL). 7.5% Palladium-carbon (0.2 g) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 4 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:1) to give the title compound (1.5494 g) as a cream color solid.
Step 5
2-Amino-4-(pyrrolidine-1-sulfonyl)phenol (1.54 g) was dissolved in N,N-dimethylformamide (9 mL), and imidazole (0.649 g) and tert-butylchlorodimethylsilane (1.254 g) were added under ice-cooling. After stirring at room temperature of 1.5 hrs, the mixture was poured into water, and the mixture was extracted with ethyl ether. The obtained ethyl ether layer was washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=7:1) to give the title compound (2.1549 g) as a white solid.
Step 6
2-(tert-Butyldimethylsilyloxy)-5-(pyrrolidine-1-sulfonyl)phenylamine (727.8 mg) was dissolved in methylene chloride (17 mL), pyridine (0.2 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (644 mg) obtained in Step 3 of Example 8 were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated under reduced pressure, and the residue was dissolved in N,N-dimethylformamide (4 mL). Potassium carbonate (1.41 g) was added at room temperature. After stirring with heating at 60° C. for 1.5 hrs, the mixture was acidified with 10% aqueous citric acid solution and water under ice-cooling. The precipitated solid was collected by filtration to give the title compound (1.0619 g) as a cream color solid.
Step 7
4-Benzyloxy-3,5-dichloro-N-[2-hydroxy-5-(pyrrolidine-1-sulfonyl)phenyl]benzamide (500 mg) was dissolved in N,N-dimethylformamide (5 mL), and potassium carbonate (332 mg) and 1,2-dibromoethane (0.099 mL) were added at room temperature. After stirring overnight at 70° C., the mixture was poured into water. The mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=2:1) to give the title compound (459.5 mg) as a white amorphous form.
Step 8
(4-Benzyloxy-3,5-dichlorophenyl)-[6-(pyrrolidine-1-sulfonyl)-2,3-dihydrobenzo[1,4]oxazin-4-yl]-methanone (442.5 mg) was dissolved in tetrahydrofuran (20 mL). 7.5% Palladium-carbon (45 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:1) to give the title compound (384.5 mg) as a white amorphous solid.
The title compound (341.8 mg) was obtained as a white amorphous solid by a method similar to Steps 2 to 8 of Example 33 and using ethylamine instead of pyrrolidine.
The title compound (379.2 mg) was obtained as a white amorphous solid by a method similar to Steps 2 to 8 of Example 33 and using dimethylamine instead of pyrrolidine.
Step 1
Lithium aluminum hydride (2 g) was suspended in tetrahydrofuran (80 mL), and 4H-pyrido[3,2-b][1,4]oxazin-3-one (3.956 g) was added under ice-cooling by small portions. After heating under reflux for 2 hrs, water (2 mL), 15% aqueous sodium hydroxide (2 mL) and water (6 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate. The solvent was evaporated, and the residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:9) to give the title compound (3.407 g) as a white solid.
Step 2
3,4-Dihydro-2H-pyrido[3,2-b][1,4]oxazine (272 mg) was dissolved in chloroform (15 mL), and triethylamine (0.335 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (662 mg) obtained in Step 3 of Example 8 were added under ice-cooling. The mixture was stirred at room temperature for 12 hrs, and the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (646 mg) as a white solid.
Step 3
(4-Benzyloxy-3,5-dichlorophenyl)-(2,3-dihydropyrido[3,2-b][1,4]oxazin-4-yl)-methanone (415 mg) was dissolved in tetrahydrofuran (10 mL). 7.5% Palladium-carbon (40 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid, was crystallized from ethyl acetate-tetrahydrofuran to give the title compound (266 mg) as white crystals.
Step 1
Acrylic acid (8.6 g) and benzene-1,2-diamine (5.4 g) were added to polyphosphoric acid (1.5 g), and the mixture was heated under reflux for 3 hrs. Water (100 mL), chloroform (200 mL) and N,N-dimethylformamide (50 mL) were added to the reaction mixture, and the mixture was extracted with chloroform. The obtained chloroform layer was washed successively with water, saturated aqueous sodium hydrogencarbonate and water, and dried over anhydrous sodium sulfate. The solvent was evaporated and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate-1:4) to give the title compound (490 mg).
Step 2
1,3,4,5-Tetrahydrobenzo[b] [1,4]diazepin-2-one (194.4 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (177.99 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3 mL), and the mixture was stirred with heating at 95° C. for 12 hrs. The reaction mixture was purified by silica gel chromatography to give the title compound (118 mg) as white crystals.
Step 1
3,5-Dichloro-2-hydroxybenzoic acid (600 mg) was suspended in toluene (6 mL), and thionyl chloride (0.275 mL) and N,N-dimethylformamide (1 drop) were added. After stirring with heating at 70° C. for 2 hrs, and the mixture was concentrated and azeotroped with toluene to give the title compound as a pale-yellow solid.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (391.7 mg) obtained in Step 2 of Example 1 and 3,5-dichloro-2-hydroxybenzoyl chloride obtained in the previous Step were dissolved in ethyl acetate (6 mL), and the mixture was stirred with heating at 90° C. for 1 hr. Ethyl acetate (6 mL) was added to the reaction mixture, and the mixture was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=85:15) to give the title compound (457 mg) as pale-yellow crystals.
Step 1
4-Hydroxy-3-trifluoromethylbenzoic acid (610 mg) was suspended in toluene (6 mL), and thionyl chloride (0.28 mL) and N,N-dimethylformamide (1 drop) were added. After stirring with heating at 70° C. for 2 hrs, the mixture was concentrated and azeotroped with toluene to give the title compound.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (400 mg) obtained in Step 2 of Example 1 and 4-hydroxy-3-trifluoromethylbenzoyl chloride obtained in the previous Step were dissolved in ethyl acetate (6 mL), and the mixture was stirred with heating at 90° C. for 1 hr. Ethyl acetate was added to the reaction mixture, and the mixture was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from ethyl acetate to give the title compound (359 mg) as crystals.
Step 1
3-Chloro-4-hydroxy-5-methoxybenzoic acid (598 mg) was suspended in toluene (6 mL), and thionyl chloride (0.28 mL) and N,N-dimethylformamide (1 drop) were added. After stirring with heating at 70° C. for 2 hrs, the mixture was concentrated and azeotroped with toluene to give the title compound.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (400 mg) obtained in Step 2 of Example 1 and 3-chloro-4-hydroxy-5-methoxybenzoyl chloride obtained in the previous Step were dissolved in ethyl acetate (6 mL), and the mixture was stirred with heating at 90° C. for 1 hr. Ethyl acetate was added to the reaction mixture, and the mixture was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from ethyl acetate to give the title compound (428 mg) as crystals.
Step 1
4-Chloro-3-hydroxybenzoic acid (510 mg) was suspended in toluene (6 mL), and thionyl chloride (0.28 mL) and N,N-dimethylformamide (1 drop) were added. After stirring with heating at 70° C. for 2 hrs, and the mixture was concentrated and azeotroped with toluene to give the title compound.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (400 mg) obtained in Step 2 of Example 1 and 4-chloro-3-hydroxybenzoyl chloride obtained in the previous Step were dissolved in ethyl acetate (6 mL), and the mixture was stirred with heating at 90° C. for 1 hr. Ethyl acetate was added to the reaction mixture, and the mixture was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from ethyl acetate to give the title compound (583 mg) as crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1, 2,6-dichloroisonicotinoic acid (384 mg) and 4-dimethylaminopyridine (269 mg) were dissolved in chloroform (12 mL), and WSC.HCl (422 mg) was added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (419.8 mg) as pale-yellow crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (135 mg) obtained in Step 2 of Example 1 and 4-nitrobenzoyl chloride (185 mg) were dissolved in ethyl acetate (3 mL), and the mixture was stirred overnight with heating at 95° C. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (288.1 mg) as a yellow solid.
Step 1
6-Fluoro-4H-benzo[1,4]oxazin-3-one (1.5 g) was dissolved in tetrahydrofuran (20 mL), borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 11 mL) was added under ice-cooling, and the mixture was stirred overnight at room temperature. 6N Hydrochloric acid (5 mL) was added, and the mixture was stirred with heating at 70° C. The mixture was allowed to cool to room temperature, weak-alkalified with 4N aqueous sodium hydroxide and saturated aqueous sodium hydrogencarbonate, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (954 mg) as an oil.
Step 2
6-Fluoro-3,4-dihydro-2H-benzo[1,4]oxazine (230 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (338 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3.5 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (374.9 mg) as pale-beige crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (300 mg) obtained in Step 2 of Example 1 and phthalic anhydride (329 mg) were dissolved in toluene (3 mL), and the mixture was heated under reflux for 4 hrs. The reaction mixture was allowed to cool, and the precipitated solid was collected by filtration to give the title compound (519 mg) as crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (154 mg) obtained in Step 2 of Example 1 was dissolved in chloroform (6 mL), triethylamine (0.18 mL) and methyl 4-chlorocarbonylbenzoate (226 mg) was added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=7:2) to give the title compound (287.4 mg) as a pale-orange solid.
Methyl 4-(2,3-dihydrobenzo[1,4]oxazine-4-carbonyl)benzoate (283 mg) obtained in Example 46 was dissolved in methanol (10 mL) and tetrahydrofuran (5 mL), and 4N aqueous lithium hydroxide (1.5 mL) was added. After stirring overnight at room temperature, the reaction mixture was concentrated under reduced pressure, and the residue was acidified with 1N hydrochloric acid under ice-cooling. The precipitated solid was collected by filtration to give the title compound (207.6 mg) as a white solid.
Step 1
Chloroform (5 mL) was added to isophthalic acid monomethyl ester (198 mg), and oxalyl chloride (0.12 mL) and N,N-dimethylformamide (1 drop) were added under ice-cooling. After stirring at room temperature for 7 hrs, the mixture was concentrated and azeotroped with toluene to give the title compound.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (154 mg) obtained in Step 2 of Example 1 was dissolved in chloroform (6 mL), and triethylamine (0.18 mL) and methyl 3-chlorocarbonylbenzoate obtained in the previous Step were added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (323.7 mg) as a white solid.
Methyl 3-(2,3-dihydrobenzo[1,4]oxazine-4-carbonyl)benzoate (293 mg) obtained in Step 2 of Example 48 was dissolved in methanol (10 mL) and tetrahydrofuran (5 mL), and 4N aqueous lithium hydroxide (1.5 mL) was added. After stirring overnight at room temperature, the reaction mixture was concentrated under reduced pressure, and the residue was acidified with 1N hydrochloric acid under ice-cooling. The precipitated solid was collected by filtration, and the obtained solid was purified by silica gel chromatography (chloroform-methanol=10:1) to give the title compound (71.8 mg) as a white amorphous solid.
Step 1
2,4-Dihydroxybenzoic acid (25.0 g) was dissolved in ethyl acetate (400 mL), tert-butyl hypochlorite (61.9 g) was added dropwise under ice-cooling, and the mixture was stirred for 2 hrs. The reaction mixture was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was crystallized from ethyl ether-n-hexane to give the title compound (11.88 g) as a solid.
Step 2
3,5-Dichloro-2,4-dihydroxybenzoic acid (605 mg) was suspended in toluene (6 mL), and thionyl chloride (0.25 mL) and N,N-dimethylformamide (1 drop) were added. After heating under reflux for 1 hr, the mixture was concentrated and azeotroped with toluene to give the title compound.
Step 3
3,4-Dihydro-2H-benzo[1,4]oxazine (366 mg) obtained in Step 2 of Example 1 and 3,5-dichloro-2,4-dihydroxybenzoyl chloride obtained in the previous Step were dissolved in ethyl acetate (6 mL), and the mixture was heated under reflux for 1 hr. The reaction mixture was washed successively with water, 1N hydrochloric acid, water, saturated aqueous sodium hydrogencarbonate, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was crystallized from ethyl acetate to give the title compound (171 mg) as crystals.
Step 1
2-Amino-4-chlorophenol (1.0061 g) was dissolved in ethyl acetate (10 mL), water (10 mL) and sodium hydrogencarbonate (1.185 g) were added under ice-cooling, and chloroacetyl chloride (0.67 mL) was added dropwise. After stirring at room temperature for 2.5 hrs, water (20 mL) was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.5455 g) as a yellow solid.
Step 2
2-Chloro-N-(5-chloro-2-hydroxyphenyl)-acetamide (1.54 g) was dissolved in N,N-dimethylformamide (15 mL), and potassium carbonate (1.26 g) was added at room temperature. After stirring overnight at room temperature, 1N hydrochloric acid (12 L) was added under ice-cooling, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.281 g) as a pale-yellow solid.
Step 3
6-Chloro-4H-benzo[1,4]oxazin-3-one (764 mg) was dissolved in tetrahydrofuran (8 mL), borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 6.2 mL) was added under ice-cooling, and the mixture was stirred overnight at room temperature. After stirring with heating at 70° C. for 1 hr, methanol (3 mL) was added dropwise at the same temperature, and the mixture was further stirred with heating for 1 hr. Then, while maintaining at 70° C., 1N hydrochloric acid (6.2 mL) was added dropwise and, after stirring with heating for 0.5 hr, the mixture was allowed to cool to room temperature. The mixture was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=99:1) to give the title compound (640.1 mg) as a solid.
Step 4
6-Chloro-3,4-dihydro-2H-benzo[1,4]oxazine (168.4 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (242.7 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3 mL), and the mixture was heated under reflux overnight. The solvent was evaporated, and the obtained solid was crystallized from methanol to give the title compound (318.4 mg) as white crystals.
The title compound (749.3 mg) was obtained as white crystals by a method similar to Steps 1 to 4 of Example 51 and using 2-amino-5-chlorophenol instead of 2-amino-4-chlorophenol.
1,2,3,4-Tetrahydroquinoxaline (68.1 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (250.5 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (5 mL), and the mixture was heated under reflux overnight. The solvent was evaporated, and the obtained solid was crystallized from methanol to give the title compound (122.0 mg) as pale-gray crystals.
Step 1
1,2,3,4-Tetrahydroquinoxaline (805.3 mg) was dissolved in ethyl acetate (30 mL), triethylamine (1.0 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (1.8988 g) obtained in Step 3 of Example 8 were added under ice-cooling, and the mixture was stirred overnight at room temperature. Methanol was added to the reaction mixture, and the mixture was concentrated. The obtained solid was crystallized from ethyl acetate-water to give the title compound (2.042 g) as a pale-yellow solid.
(4-Benzyloxy-3,5-dichlorophenyl)-(3,4-dihydro-2H-quinoxalin-1-yl)-methanone. (287.7 mg) was dissolved in tetrahydrofuran (6 mL). 7.5% Palladium-carbon (26 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained solid was crystallized from methanol to give the title compound (194.1 mg) as pale-yellow crystals.
Step 1
Methanol (50 mL) and concentrated sulfuric acid (0.5 mL) were added to 4-hydroxy-3-nitrobenzoic acid (5.0022 g), and the mixture was stirred overnight at 80° C. The solvent was evaporated, and the residue was partitioned between ethyl acetate and saturated aqueous sodium hydrogencarbonate. The aqueous layer was acidified with 1N hydrochloric acid, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (5.3436 g) as a yellow solid.
Step 2
Methyl 4-hydroxy-3-nitrobenzoate (5.3436 g) was dissolved in tetrahydrofuran (27 mL) and methanol (27 mL). 7.5% Palladium-carbon (276.6 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure to give the title compound (4.6803 g) as a solid.
Step 3
Methyl 3-amino-4-hydroxybenzoate (4.6803 g) and benzyltriethylammonium chloride (6.1787 g) were suspended in chloroform (50 mL), sodium hydrogencarbonate (9.10 g) and chloroacetyl chloride (2.6 mL) were added under ice-cooling, and the mixture was stirred under ice-cooling for 1 hr. Thereafter, the mixture was stirred with heating at 70° C. The reaction mixture was concentrated, water and ethyl acetate were added, and the precipitated solid was collected by filtration. The mother liquor was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue and the solid collected earlier by filtration were combined and the mixture was crystallized from methanol to give the title compound (4.8085 g) as a solid.
Step 4
Methyl 3-oxo-3,4-dihydro-2H-benzo[1,4]oxazine-6-carboxylate (1.009 g) was added to borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 11 mL) under ice-cooling, and the mixture was stirred with heating at 70° C. for 3.5 hrs. Methanol (3 mL) was added dropwise and the mixture was further stirred with heating for 3 hrs. The mixture was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=98:2) to give the title compound (281.3 mg) as a pink solid and (3,4-dihydro-2H-benzo[1,4]oxazin-6-yl)methanol (413.8 mg) as an oil.
Step 5
Methyl 3,4-dihydro-2H-benzo[1,4]oxazine-6-carboxylate (81.2 mg) was dissolved in ethyl acetate (3 mL), 3,5-dichloro-4-hydroxybenzoyl chloride (108.3 mg) obtained in Step 1 of Example 3 was added under ice-cooling, and the mixture was stirred overnight at 80° C. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (167.8 mg) as a white amorphous solid.
Step 1
(3,4-Dihydro-2H-benzo[1,4]oxazin-6-yl)methanol (406.1 mg) obtained in Step 4 of Example 55 was dissolved in N,N-dimethylformamide (4 mL), and imidazole(203.8 mg) and tert-butylchlorodimethylsilane (443.4 mg) were added under ice-cooling. After stirring at room temperature for 1 hr, the mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (659.0 mg) as a pale-yellow oil.
Step 2
6-(tert-Butyldimethylsilyloxymethyl)-3,4-dihydro-2H-benzo[1,4]oxazine (279.1 mg) was dissolved in ethyl acetate (3 mL), triethylamine (0.167 mL) and 3,5-dichloro-4-hydroxybenzoyl chloride (249.4 mg) obtained in Step 1 of Example 3 were added under ice-cooling, and the mixture was stirred overnight at 80° C. The solvent was evaporated, and water and ethyl acetate were added and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (131.7 mg) as a pale-yellow solid.
Step 3
[6-(tert-Butyldimethylsilyloxymethyl)-2,3-dihydrobenzo[1,4]oxazin-4-yl]-(3,5-dichloro-4-hydroxyphenyl)-methanone (131.7 mg) was dissolved in tetrahydrofuran (1.5 mL), 1M tetrabutylammonium fluoride/tetrahydrofuran solution (0.34 mL) was added under ice-cooling, and the mixture was stirred overnight at room temperature. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=97:3) to give the title compound (50.0 mg) as crystals.
Methyl 4-(3,5-dichloro-4-hydroxybenzoyl)-3,4-dihydro-2H-benzo[1,4]oxazine-6-carboxylate (140.8 mg) obtained in Step 5 of Example 55 was dissolved in methanol (1.5 mL) and tetrahydrofuran (1.5 mL), and 2N aqueous sodium hydroxide (0.55 mL) was added. After stirring overnight at room temperature, the solvent was evaporated, and the residue was acidified with 10% aqueous citric acid solution under ice-cooling. The precipitated solid was collected by filtration, and the obtained solid was crystallized from methanol to give the title compound (81.3 mg) as crystals.
The title compound (246.0 mg) was obtained as a white amorphous solid by a method similar to Steps 1 to 5 of Example 55 and using 3-hydroxy-2-nitrobenzoic acid instead of 4-hydroxy-3-nitrobenzoic acid.
The title compound (317.9 mg) was obtained as white crystals by a method similar to Steps 1 to 5 of Example 55 and using 3-hydroxy-4-nitrobenzoic acid instead of 4-hydroxy-3-nitrobenzoic acid.
Methyl 4-(3,5-dichloro-4-hydroxybenzoyl)-3,4-dihydro-2H-benzo[1,4]oxazine-7-carboxylate (257.4 mg) obtained in Example 59 was dissolved in methanol (2.5 mL) and tetrahydrofuran (2.5 mL), and 2N aqueous sodium hydroxide (1.0 mL) was added. After stirring with heating at 60° C. for 2.5 hrs, the solvent was evaporated, and the residue was acidified with 10% aqueous citric acid solution under ice-cooling. The precipitated solid was collected by filtration, and the obtained solid was crystallized from methanol to give the title compound (76.6 mg) as crystals.
The title compound (313.8 mg) was obtained as white crystals by a method similar to Steps 1 to 5 of Example 55 and using 2-hydroxy-3-nitrobenzoic acid instead of 4-hydroxy-3-nitrobenzoic acid.
Methyl 4-(3,5-dichloro-4-hydroxybenzoyl)-3,4-dihydro-2H-benzo[1,4]oxazine-8-carboxylate (244.2 mg) obtained in Example 61 was dissolved in methanol (2.5 mL) and tetrahydrofuran (2.5 mL), and 2N aqueous sodium hydroxide (0.96 mL) was added. After stirring overnight at room temperature, the solvent was evaporated, and the residue was acidified with 10% aqueous citric acid solution under ice-cooling. The precipitated solid was collected by filtration, and the obtained solid was crystallized from ethyl acetate to give the title compound (186.6 mg) as crystals.
3,4-Dihydro-2H-benzo[1,4]oxazine (250.1 mg) obtained in Step 2 of Example 1 and 3,5-dichlorobenzoyl chloride (426.9 mg) were dissolved in ethyl acetate (5 mL), and the mixture was stirred overnight with heating at 80° C. The solvent was evaporated, and the obtained residue was crystallized from methanol to give the title compound (469.6 mg) as pale-beige crystals.
Phenoxazine (276.1 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (370.8 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (3 mL), and the mixture was stirred overnight with heating at 80° C. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (397.6 mg) as a yellow green solid.
The title compound (160.0 mg) was obtained as white crystals by a method similar to Steps 2 to 5 of Example 21 and using 2-amino-4-phenylphenol instead of 3-amino-N,N-diethyl-4-hydroxybenzenesulfonamide.
The title compound (152.7 mg) was obtained as crystals by a method similar to Steps 1 to 4 of Example 51 and using 2-amino-4,6-dimethylphenol instead of 2-amino-4-chlorophenol.
Step 1
2-Amino-4-nitrophenol (4.6283 g) and benzyltriethylammonium chloride (6.8772 g) were suspended in chloroform (46 mL), sodium hydrogencarbonate (10.10 g) and chloroacetyl chloride (4.0707 g) were added under ice-cooling, and the mixture was stirred for 1 hr under ice-cooling. Thereafter, the mixture was stirred with heating at 70° C. The reaction mixture was concentrated, water and ethyl acetate were added, and the precipitated solid was collected by filtration. The mother liquor was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, the obtained residue and the solid collected earlier by filtration were combined and the mixture was crystallized from ethanol to give the title compound (5.4344 g) as a solid.
Step 2
To borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 33.5 mL) was added 6-nitro-4H-benzo[1,4]oxazin-3-one (3.0084 g) under ice-cooling, and the mixture was stirred with heating at 70° C. for 5 hrs. Methanol (5 mL) was added dropwise, and the mixture was further stirred with heating for 2.5 hrs. Concentrated hydrochloric acid (5 mL) was added dropwise, and the mixture was stirred with heating for 1.5 hrs. The mixture was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was crystallized from n-hexane-ethyl acetate to give the title compound (2.2092 g) as a solid.
Step 3
6-Nitro-3,4-dihydro-2H-benzo[1,4]oxazine (376.1 mg) was dissolved in ethyl acetate (3 mL), 3,5-dichloro-4-hydroxybenzoyl chloride (270.7 mg) obtained in Step 1 of Example 3 was added under ice-cooling, and the mixture was stirred overnight at 80° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (538.0 mg) as pale-yellow crystals.
(3,5-Dichloro-4-hydroxyphenyl)-(6-nitro-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (200.0 mg) obtained in Step 3 of Example 67 was dissolved in tetrahydrofuran (12 mL). 7.5% Palladium-carbon (19.4 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (6.4 mg) as crystals.
6-Nitro-3,4-dihydro-2H-benzo[1,4]oxazine (179.8 mg) obtained in Step 2 of Example 67 was dissolved in ethyl acetate (5 mL), 3,5-dibromo-4-hydroxybenzoyl chloride (346.6 mg) obtained in Step 1 of Example 4 was added under ice-cooling, and the mixture was stirred overnight at 80° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (437.0 mg) as yellow crystals.
The title compound (485.2 mg) was obtained as yellow crystals by a method similar to Steps 1 to 3 of Example 67 and using 2-amino-5-nitrophenol instead of 2-amino-4-nitrophenol.
(3,5-Dichloro-4-hydroxyphenyl)-(7-nitro-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (303.1 mg) obtained in Example 70 was dissolved in tetrahydrofuran (6 mL) and methanol (3 mL). 7.5% Palladium-carbon (30 mg) was added to this solution and, under a hydrogen atmosphere, and the mixture was stirred at room temperature for 1.5 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was crystallized from ethanol to give the title compound (257.9 mg) as yellow crystals.
(7-Amino-2,3-dihydrobenzo[1,4]oxazin-4-yl)-(3,5-dichloro-4-hydroxyphenyl)-methanone (251.7 mg) obtained in Example 71 was suspended in methylene chloride (5 mL), pyridine (0.0776 mL) and methanesulfonyl chloride (0.0689 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. Methanol was added to the reaction mixture, and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (173.6 mg) as a pale-orange amorphous solid.
Step 1
(4-Benzyloxy-3,5-dichlorophenyl)-(3,4-dihydro-2H-quinoxalin-1-yl)-methanone (400 mg) obtained in Step 1 of Example 54 was suspended in methylene chloride (8 mL), triethylamine (0.162 mL) and acetyl chloride (0.082 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. Methanol was added to the reaction mixture, and the mixture was concentrated under reduced pressure. Water was added and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=99:1) to give the title compound (295.9 mg) as a pale-yellow amorphous form.
Step 2
1-[4-(4-Benzyloxy-3,5-dichlorobenzoyl)-3,4-dihydro-2H-quinoxalin-1-yl]-ethanone (288.6 mg) was dissolved in tetrahydrofuran (6 mL). 7.5% Palladium-carbon (27.7 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (chloroform-methanol=97:3) and crystallized from n-hexane-ethyl acetate to give the title compound (108.1 mg) as white crystals.
Step 1
(4-Benzyloxy-3,5-dichlorophenyl)-(3,4-dihydro-2H-quinoxalin-1-yl)-methanone (401.6 mg) obtained in Step 1 of Example 54 was dissolved in N,N-dimethylformamide (8 mL), potassium carbonate (335.7 mg) and iodomethane(0.076 mL) were added, and the mixture was stirred with heating at 50° C. The mixture was poured into water, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (chloroform-methanol=99:1) to give the title compound (114.7 mg) as an amorphous form.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(4-methyl-3,4-dihydro-2H-quinoxalin-1-yl)-methanone (114.7 mg) was dissolved in tetrahydrofuran (5 mL). 7.5% Palladium-carbon (10.0 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was crystallized from ethyl acetate to give the title compound (56.3 mg) as pale-yellow crystals.
Step 1
1,2-Dimethoxyethane (20 mL) was added to 4-hydroxy-3-nitrobenzoic acid (1.83 g) to dissolve same by heating the mixture to 80° C. Thionyl chloride (1.1 mL) was added, and the mixture was stirred overnight at 80° C. The reaction mixture was concentrated under reduced pressure, and azeotroped with toluene to give the title compound (2.0551 g) as a yellow oil.
Step 2
3,4-Dihydro-2H-benzo[1,4]oxazine (203 mg) obtained in Step 2 of Example 1 and 4-hydroxy-3-nitrobenzoyl chloride (302 mg) were dissolved in ethyl acetate (2 mL), and the mixture was heated under reflux overnight. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (460.7 mg) as a yellow amorphous solid.
Step 1
2-Methyl-2,3-dihydro-1H-indole (139.3 mg) was dissolved in methylene chloride (2.5 mL), pyridine (0.1 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (396.6 mg) obtained in Step 3 of Example 8 were added, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated, water was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=6:1) to give the title compound (449.4 mg) as a yellow oil.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(2-methyl-2,3-dihydroindol-1-yl)-methanone (437.1 mg) was dissolved in toluene (4 mL), and trifluoroacetic acid (2.5 mL) was added at room temperature. After stirring with heating at 80° C., the mixture was concentrated. The obtained solid was crystallized from n-hexane-ethyl acetate to give the title compound (300.3 mg) as white crystals.
The title compound (230.6 mg) was obtained as white crystals by a method similar to Steps 1 and 2 of Example 76 and using 2,3-dihydro-1H-indole instead of 2-methyl-2,3-dihydro-1H-indole.
Step 1
5-Nitro-2,3-dihydro-1H-indole (329.5 mg) was dissolved in methylene chloride (5 mL), pyridine (0.194 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (762.1 mg) obtained in Step 3 of Example 6 were added, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated, water was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1) to give the title compound (539.7 mg) as a yellow solid.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(5-nitro-2,3-dihydroindol-1-yl)-methanone (539.7 mg) was dissolved in tetrahydrofuran (10 mL). Platinum oxide (IV) (14 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. Water was added, and the mixture was extracted with chloroform. The obtained chloroform layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give a mixture (489.5 mg) of the title compound and (5-amino-2,3-dihydroindol-1-yl)-(3,5-dichloro-4-hydroxyphenyl)-methanone as a yellow amorphous form.
Step 3
A mixture (489.5 mg) of (5-amino-2,3-dihydroindol-1-yl)-(4-benzyloxy-3,5-dichlorophenyl)-methanone and (5-amino-2,3-dihydroindol-1-yl)-(3,5-dichloro-4-hydroxyphenyl)-methanone was dissolved in toluene (5 mL), and trifluoroacetic acid (2.75 mL) was added at room temperature. After stirring with heating at 80° C. for 2.5 hrs, the mixture was concentrated. The obtained solid was crystallized from ethyl acetate to give the title compound (282.4 mg) as pale-gray crystals.
6-Fluoro-3,4-dihydro-2H-benzo[1,4]oxazine (230 mg) obtained in Step 1 of Example 44 and 3,5-dibromo-4-hydroxybenzoyl chloride (472 mg) obtained in Step 1 of Example 4 were dissolved in ethyl acetate (3.5 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (533.7 mg) as white crystals.
2,3-Dihydro-1H-naphtho[2,1-b][1,4]oxazine (140.9 mg) obtained in Step 3 of Example 16 and 3,5-dibromo-4-hydroxybenzoyl chloride (239 mg) obtained in Step 1 of Example 4 were dissolved in ethyl acetate (2.3 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (298.8 mg) as black blue crystals.
6-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (149 mg) obtained in Step 1 of Example 12 and 3,5-dibromo-4-hydroxybenzoyl chloride (314 mg) obtained in Step 1 of Example 4 were dissolved in ethyl acetate (3.5 mL), and the mixture was stirred overnight at 95° C. The reaction mixture was allowed to cool to room temperature, and the precipitated solid was collected by filtration to give the title compound (368.2 mg) as beige crystals.
6-Chloro-3,4-dihydro-2H-benzo[1,4]oxazine (170.6 mg) obtained in Step 3 of Example 51 and 3,5-dibromo-4-hydroxybenzoyl chloride (349.3 mg) obtained in Step 1 of Example 4 were dissolved in ethyl acetate (5 mL), and the mixture was heated under reflux overnight. The solvent was evaporated, and the obtained solid was crystallized from methanol to give the title compound (441.0 mg) as crystals.
Step 1
(4-Benzyloxy-3,5-dichlorophenyl)-(3,4-dihydro-2H-quinoxalin-1-yl)-methanone (399.7 mg) obtained in Step 1 of Example 54 was dissolved in methylene chloride (8 mL), triethylamine (0.162 mL) and methanesulfonyl chloride (0.094 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was concentrated, water was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=98:2) to give the title compound (451.5 mg) as a pale-yellow amorphous form.
Step 2
(4-Benzyloxy-3,5-dichlorophenyl)-(4-methanesulfonyl-3,4-dihydro-2H-quinoxalin-1-yl)-methanone (451.5 mg) was dissolved in tetrahydrofuran (10 mL). 7.5% Palladium-carbon (43.1 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (335.9 mg) as a pale-yellow amorphous solid.
Step 1
2-Amino-4-ethanesulfonylphenol (1.5063 g) was dissolved in ethyl acetate (15 mL), water (15 mL) and sodium hydrogencarbonate (1.2588 g) were added under ice-cooling, and chloroacetyl chloride (0.715 mL) was added dropwise. After stirring at room temperature for 5 hrs, water (20 mL) was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.9375 g) as a brown solid.
Step 2
2-Chloro-N-(5-ethanesulfonyl-2-hydroxyphenyl)acetamide (1.9375 g) was dissolved in N,N-dimethylformamide (20 mL), and potassium carbonate (1.2520 g) was added at room temperature. After stirring overnight at room temperature, water (20 mL) was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained solid was crystallized from methanol to give the title compound (1.3358 g) as a solid.
Step 3
6-Ethanesulfonyl-4H-benzo[1,4]oxazin-3-one (802.3 mg) was dissolved in tetrahydrofuran (4 mL), and borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 7 mL) was added under ice-cooling. After stirring with heating at 70° C. for 9 hrs, methanol (5 mL) was added dropwise at the same temperature, and the mixture was further stirred with heating for 1 hr. 1N Hydrochloric acid (5 mL) was added dropwise while heating at 70° C. and, after stirring with heating for 1 hr, the mixture was allowed to cool to room temperature. The mixture was extracted with ethyl acetate, and the obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (748.7 mg) as an orange oil.
Step 4
6-Ethanesulfonyl-3,4-dihydro-2H-benzo[1,4]oxazine (233.5 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (255.4 mg) obtained Step 1 of Example 3 were dissolved in ethyl acetate (5 mL), and the mixture was heated under reflux overnight. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (446.9 mg) as a white amorphous solid.
Step 1
2-Nitro-4-trifluoromethylphenol (3.0951 g) was dissolved in tetrahydrofuran (15 mL). 7.5% Palladium-carbon (299.8 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure to give the title compound (2.6346 g) as a gray solid.
Step 2
2-Amino-4-trifluoromethylphenol (1.0075 g) was dissolved in ethyl acetate (10 mL), water (10 mL) and sodium hydrogencarbonate (0.9531 g) were added under ice-cooling, and chloroacetyl chloride (0.55 mL) was added dropwise. The mixture was stirred overnight at room temperature, and extracted with ethyl acetate. The obtained ethyl acetate layer was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.4341 g) as a pale-brown solid.
Step 3
2-Chloro-N-(2-hydroxy-5-trifluoromethylphenyl)acetamide (1.4244 g) was dissolved in N,N-dimethylformamide (14 mL), and potassium carbonate (1.0072 g) was added at room temperature. After stirring at room temperature for 2 hrs, water (20 mL) was added, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (1.2084 g) as a pale-gray solid.
Step 4
6-Trifluoromethyl-4H-benzo[1,4]oxazin-3-one (810.8 mg) was dissolved in tetrahydrofuran (4 mL), borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 7.4 mL) was added under ice-cooling. After stirring with heating at 70° C. for 6 hrs, methanol (5 mL) was added dropwise at the same temperature, and the mixture was further stirred with heating for 1 hr. 1N Hydrochloric acid (5 mL) was added dropwise while heating at 70° C., and the mixture was stirred with heating for 30 min. The mixture was allowed to cool to room temperature and extracted with ethyl acetate. The obtained ethyl acetate layer was washed with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=99:1) to give the title compound (718.3 mg) as a white solid.
Step 5
6-Trifluoromethyl-3,4-dihydro-2H-benzo[1,4]oxazine (205.3 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (252.5 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (5 mL), and the mixture was heated under reflux overnight. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (chloroform-methanol=95:5) to give the title compound (286.5 mg) as white crystals.
(3,5-Dichloro-4-hydroxyphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (100 mg) obtained in Step 2 of Example 3 was dissolved in acetone (5 mL), potassium carbonate (47 mg) and iodomethane(0.096 mL) were added, and the mixture was stirred with heating at 45° C. overnight. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (109.2 mg) as a white solid.
(3,5-Dichloro-4-hydroxyphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (100 mg) obtained in Step 2 of Example 3 was dissolved in chloroform (5 mL), triethylamine (0.064 mL) and acetyl chloride (0.026 mL) were added under ice-cooling, and the mixture was stirred overnight at room temperature. The reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=5:1) to give the title compound (123.1 mg) as white crystals.
Step 1
3,4-Dihydro-2H-benzo[1,4]oxazine (270 mg) obtained in Step 2 of Example 1, 4-benzyloxybenzoic acid (457 mg) and 4-dimethylaminopyridine (269 mg) were dissolved in chloroform (7 mL), and WSC.HCl (422 mg) was added under ice-cooling. After stirring overnight at room temperature, the reaction mixture was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (636.2 mg) as an orange oil.
Step 2
(4-Benzyloxyphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (630 mg) was dissolved in tetrahydrofuran (20 mL). 7.5% Palladium-carbon (70 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 6 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The filtrate and washing solution were combined and the mixture was concentrated under reduced pressure. The obtained residue was crystallized from ethyl acetate to give the title compound (149.1 mg) as white crystals.
Step 1
2-Aminobenzene-1,3-diol hydrochloride (2.00 g) was dissolved in ethyl acetate (20 mL), water (20 mL) and sodium hydrogencarbonate (2.50 g) were added, chloroacetyl chloride (1.02 mL) was added dropwise under ice-cooling. After stirring at room temperature for 12 hrs, and the mixture was extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=65:35) to give the title compound (1.508 g) as a pale-orange solid.
Step 2
2-Chloro-N-(2,6-dihydroxyphenyl)-acetamide (1.274 g) was dissolved in N,N-dimethylformamide (10 mL), potassium carbonate (873 mg) was added at room temperature. After stirring at room temperature for 2.5 hrs, water (30 mL) was added. The mixture was stirred under ice-cooling for 0.5 hr, and the precipitated solid was collected by filtration to give the title compound (915 mg) as a pale-orange solid.
Step 3
5-Hydroxy-4H-benzo[1,4]oxazin-3-one (200 mg) was dissolved in N,N-dimethylformamide (2 mL), potassium carbonate (167 mg) and benzyl bromide (158 mL) were added at room temperature. The mixture was stirred at room temperature for 3 hrs and stirred with heating at 60° C. for 1 hr, and water was added. The precipitated solid was collected by filtration to give the title compound (283 mg) as a pale-yellow solid.
Step 4
Lithium aluminum hydride (50 mg) was suspended in tetrahydrofuran (2 mL), and 5-benzyloxy-4H-benzo[1,4]oxazin-3-one (276 mg) was added by small portions under ice-cooling. The mixture was stirred at room temperature for 1 hr and stirred with heating at 60° C. for 1 hr. Water (0.05 mL), 15% aqueous sodium hydroxide (0.05 mL) and water (0.15 mL) were successively added under ice-cooling, and the mixture was stirred at room temperature. The mixture was dried over anhydrous sodium sulfate and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (54 mg) as an oil.
Step 5
5-Benzyloxy-3,4-dihydro-2H-benzo[1,4]oxazine (47 mg) was dissolved in chloroform (2 mL), triethylamine (0.0716 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (689.3 mg) obtained in Step 3 of Example 8 were added. The mixture was stirred at room temperature for 25 hrs, water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The mixture was washed successively with water, saturated aqueous sodium hydrogencarbonate and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=85:15) to give the title compound (54.7 mg) as a white solid.
Step 6
(4-Benzyloxy-3,5-dichlorophenyl)-(5-benzyloxy-2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (52 mg) was dissolved in tetrahydrofuran (2 mL). 7.5% Palladium-carbon (10 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 1 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The solvent was evaporated and the obtained solid was crystallized from ethyl ether to give the title compound (14.8 mg) as a pale-yellow solid.
Step 1
1,2-Dimethoxyethane (100 mL) was added to 2-methoxyphenol (6.21 g), and the mixture was cooled to −50° C. Nitronium tetrafluoroborate (6.77 g) was added, and the mixture was stirred at −50° C. After the completion of reaction, the reaction mixture was poured into ice water, and ethyl acetate and ethyl ether were added. The insoluble material was removed by filtration, and the filtrate was extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1-3:1) to give the title compound (2.46 g) as a yellow solid.
Step 2
2-Methoxy-6-nitrophenol (2.46 g) was dissolved in tetrahydrofuran (20 mL). 7.5% Palladium-carbon (400 mg) was added to this solution and, under a hydrogen atmosphere, and the mixture was stirred at room temperature for 7 hrs. The reaction mixture was filtered through celite, and the residue was washed with ethyl acetate. Under ice-cooling, 4N hydrogen chloride-ethyl acetate (10 mL) was added dropwise and the mixture was stirred for 30 min. The precipitated solid was collected by filtration to give the title compound (2.41 g) as a white solid.
Step 3
2-Amino-6-methoxyphenol hydrochloride (2.40 g) was dissolved in ethyl acetate (25 mL), water (30 mL) and sodium hydrogencarbonate (2.76 g) were added under ice-cooling, and chloroacetyl chloride (1.2 mL) was added dropwise. The mixture was stirred at room temperature for 0.5 hr, and extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (2.95 g) as a pale-orange solid.
Step 4
2-Chloro-N-(2-hydroxy-3-methoxyphenyl)-acetamide (2.95 g) was dissolved in N,N-dimethylformamide (20 mL), and potassium carbonate (2.46 g) was added under ice-cooling. The mixture was stirred at room temperature for 4 hrs, and water was added. The mixture was stirred at room temperature for 0.5 hr, and the precipitated solid was collected by filtration to give the title compound (2.06 g) as a pink solid.
Step 5
8-Methoxy-4H-benzo[1,4]oxazin-3-one (950 mg) was dissolved in methylene chloride (90 mL). After cooling to −78° C., boron tribromide (1.0M methylene chloride solution, 13.3 mL) was added dropwise, and the mixture was stirred at room temperature for 2 hrs. Under ice-cooling, the reaction mixture was poured into water, and the mixture was extracted with ethyl acetate. The extract was washed with saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (864 mg) as a khaki solid.
Step 6
8-Hydroxy-4H-benzo[1,4]oxazin-3-one (900 mg) was dissolved in N,N-dimethylformamide (10 mL), and imidazole (482 mg) and tert-butylchlorodimethylsilane (986 mg) were added. The mixture was stirred at room temperature for 1 hr, and water and 10% aqueous citric acid solution were added. The mixture was extracted with ethyl acetate, washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=7:3) to give the title compound (1.41 g) as a white solid.
Step 7
8-(tert-Butyldimethylsilyloxy)-4H-benzo[1,4]oxazin-3-one (700 mg) was dissolved in tetrahydrofuran (5 mL), borane-tetrahydrofuran complex (1M tetrahydrofuran solution, 3.76 mL) was added, and the mixture was heated under reflux for 2.5 hrs. The mixture was allowed to cool to room temperature, weak-alkalified with saturated aqueous sodium hydrogencarbonate and water, and extracted with ethyl acetate. The obtained ethyl acetate layer was washed successively with water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (675 mg) as an oil.
Step 8
8-(tert-Butyldimethylsilyloxy)-3,4-dihydro-2H-benzo[1,4]oxazine (792 mg) was dissolved in chloroform (10 mL), and pyridine (0.242 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (792 mg) obtained in Step 3 of Example 8 were added. The mixture was stirred at room temperature for 1 hr, 10% aqueous citric acid solution was added, and the mixture was extracted with chloroform. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=8:1) to give the title compound (1.28 g) as a white solid.
Step 9
(4-Benzyloxy-3,5-dichlorophenyl)-[8-(tert-butyldimethylsilyloxy)-2,3-dihydrobenzo[1,4]oxazin-4-yl]-methanone (545 mg) was dissolved in toluene (2 mL), and trifluoroacetic acid (4 mL) was added at room temperature. After stirring with heating at 85° C. for 7.5 hrs, and the mixture was concentrated. The obtained residue was dissolved in tetrahydrofuran, and the solution was treated with activated carbon. The solvent was evaporated, and the residue was purified by silica gel chromatography (n-hexane-ethyl acetate=1:1) and crystallized from ethyl ether was to give the title compound (220 mg) as a yellow solid.
(3,5-Dichloro-4-hydroxyphenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (1.0067 g) obtained in Example 3 was dissolved in N,N-dimethylformamide (10 mL), and potassium carbonate (0.6553 g) and ethyl bromoacetate (0.52 mL) were added at room temperature. After stirring overnight with heating at 70° C., ethyl acetate (20 mL) and 10% aqueous citric acid solution (20 mL) were added under ice-cooling, and the mixture was extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (1.2891 g) as a pale-yellow oil.
Ethyl [2,6-dichloro-4-(2,3-dihydrobenzo[1,4]oxazine-4-carbonyl)phenoxy]acetate (1.2753 g) obtained in Example 91 was dissolved in methanol (6.5 mL), 2N aqueous sodium hydroxide (3.1 mL) was added, and the mixture was stirred at room temperature for 0.5 hr. Water was added to the reaction mixture, the mixture was washed with ethyl ether, and acidified with 10% aqueous citric acid solution. The mixture was extracted with ethyl acetate. The extract was washed with saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was crystallized from methanol to give the title compound (156.4 mg) as white crystals.
Step 1
2-Nitrophenol (2.78 g) was dissolved in N,N-dimethylformamide (20 mL), potassium carbonate (3.34 g) and bromoacetone (1.85 mL) were added at room temperature, and the mixture was stirred overnight at room temperature. Water was added to the reaction mixture, and the mixture was extracted with ethyl ether. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (3.2101 g) as a bright yellow solid.
Step 2
1-(2-Nitrophenoxy)propan-2-one (501.1 mg) was dissolved in tetrahydrofuran (10 mL). 7.5% Palladium-carbon (49.1 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 3 hrs. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=3:1) to give the title compound (248.1 mg) as a yellow oil.
Step 3
3-Methyl-3,4-dihydro-2H-benzo[1,4]oxazine (244.9 mg) and 3,5-dichloro-4-hydroxybenzoyl chloride (370.1 mg) obtained in Step 1 of Example 3 were dissolved in ethyl acetate (7 mL), and the mixture was stirred with heating at 80° C. for 3 hrs. Ethyl acetate (15 mL) and water (20 mL) were added to partition the reaction mixture into layers, and the aqueous layer extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained solid was crystallized from methanol to give the title compound (320.2 mg) as white crystals.
Step 1
(4-Amino-3,5-dichlorophenyl)-(2,3-dihydrobenzo[1,4]oxazin-4-yl)-methanone (323 mg) obtained in Example 28 was dissolved in N,N-dimethylformamide (5 mL), 60% sodium hydride (52 mg) was added, and the mixture was stirred at room temperature for 0.5 hr. Methanesulfonyl chloride (0.0851 mL) was added, and the mixture was stirred at room temperature. 10% Aqueous citric acid solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate and tetrahydrofuran. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=4:1), and crystallized from isopropyl ether to give the title compound (78 mg) as a white solid.
Step 2
N-Methanesulfonyl-N-[2,6-dichloro-4-(2,3-dihydrobenzo[1,4]oxazine-4-carbonyl)phenyl]methanesulfonamide (73 mg) was dissolved in tetrahydrofuran (3 mL), tetrabutylammonium fluoride (1M tetrahydrofuran solution, 0.168 mL) was added, and the mixture was stirred overnight at room temperature. 10% Aqueous citric acid solution and water added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was crystallized from ethyl acetate to give the title compound (48 mg) as a white solid.
Step 1
2-Nitrophenol (3 g) was dissolved in N,N-dimethylformamide (20 mL), and sodium hydride (1.04 g) was added under ice-cooling. The mixture was stirred at room temperature for 0.5 hr, (3-bromopropoxy)-tert-butyldimethylsilane (5.49 mL) was added under ice-cooling, and the mixture was stirred with heating at 90° C. overnight. Potassium carbonate (1 g) and (3-bromopropoxy)-tert-butyldimethylsilane (2.5 mL) were added, and the mixture was stirred with heating at 90° C. for 1.5 hrs. 10% Aqueous citric acid solution and water were added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (6.71 g) as a yellow oil.
Step 2
tert-Butyldimethyl[3-(2-nitrophenoxy)propoxy]silane (6.71 g) was dissolved in tetrahydrofuran (50 mL). 7.5% Palladium-carbon (1 g) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred overnight at room temperature. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The solvent was evaporated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (5.88 g) as a pale-orange oil.
Step 3
2-[3-(tert-Butyldimethylsilyloxy)propoxy]phenylamine (844 mg) was dissolved in chloroform (10 mL), and pyridine (0.314 mL) and 4-benzyloxy-3,5-dichlorobenzoyl chloride (947 mg) obtained in Step 3 of Example 8 were added. The mixture was stirred at room temperature for 1 hr. The reaction mixture was concentrated, and the obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (1.63 g) as a pale-yellow oil.
Step 4
4-Benzyloxy-N-{2-[3-(tert-butyldimethylsilyloxy)propoxy]phenyl}-3,5-dichlorobenzamide (1.62 g) was dissolved in tetrahydrofuran (10 mL), tetrabutylammonium fluoride (1M tetrahydrofuran solution, 4.33 mL) was added, and the mixture was stirred at room temperature for 1 hr. 10% Aqueous citric acid solution was added to the reaction mixture, and extracted with ethyl acetate and tetrahydrofuran. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was crystallized from isopropyl ether to give the title compound (1.19 g) as a white solid.
Step 5
4-Benzyloxy-3,5-dichloro-N-[2-(3-hydroxypropoxy)phenyl]benzamide (1.00 g) was dissolved in pyridine (10 mL), methanesulfonyl chloride (0.225 mL) was added, and the mixture was stirred with heating at 70° C. for 2 hrs. Water was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with 10% aqueous citric acid solution, water and saturated brine, and dried over anhydrous sodium sulfate. The solvent was evaporated to give the title compound (713 mg) as a beige solid.
Step 6
4-Benzyloxy-3,5-dichloro-N-[2-(3-chloropropoxy)phenyl]benzamide (200 mg) was dissolved in N,N-dimethylformamide (3 mL), 60% sodium hydride (21 mg) and sodium iodide (64 mg) were added, and the mixture was stirred with heating at 60° C. 10% Aqueous citric acid solution was added to the reaction mixture, and the mixture was extracted with ethyl acetate. The extract was washed successively with water and saturated brine, dried over anhydrous sodium sulfate, and concentrated. The obtained residue was purified by silica gel chromatography (n-hexane-ethyl acetate=9:1) to give the title compound (121 mg) as an oil.
Step 7
(4-Benzyloxy-3,5-dichlorophenyl)-(7,8-dihydro-6H-5-oxa-9-azabenzocyclohepten-9-yl)-methanone (115 mg) was dissolved in tetrahydrofuran (5 mL). 7.5% Palladium-carbon (15 mg) was added to this solution and, under a hydrogen atmosphere, the mixture was stirred at room temperature for 0.5 hr. The reaction mixture was filtered through celite, and the residue was washed with tetrahydrofuran. The solvent was evaporated and the obtained residue was crystallized from isopropyl ether to give the title compound (69 mg) as a white solid.
The 1H-NMR spectrum data of the compounds of Examples 1-95 are shown in Table 1-Table 18.
The 1H-NMR spectrum was measured in CDCl3 or DMSO-d6, with tetramethylsilane as an inner standard, and the total δ value are shown in ppm.
The symbols in the tables mean the following.
J: coupling constant
The powder X-ray diffraction patterns of the crystals of the present invention are shown in
Based on the powder X-ray diffraction patterns, the diffraction angles (2θ) of the characteristic diffraction peaks of each crystal are listed in Table 19-Table 22.
Each sample crystal of the present invention was mounted on an aluminum cell, and the powder X-ray diffraction pattern was measured using a powder X-ray diffractometer (RINT2100 Ultima+, manufactured by Rigaku) at X-ray radiation: Cu—Kα1 ray, applied voltage: 40 kV, applied electric current: 40 mA, scanning rate: 5° per min, scanning step: 0.02°, scanning range: 5°-40°. Since a diffraction peak due to aluminum cell is observed at around 38.20°-38.40°, the diffraction peaks near this range are not listed as a characteristic diffraction peak of each crystal.
In general, diffraction angle (2θ) and peak intensity (cps) of powder X-ray diffraction pattern may vary depending on a measurement device, measurement conditions, and so on. The crystals in the present specification may show diffraction angle (2θ) and peak intensity of powder X-ray diffraction pattern different from those described in the present specification, as long as it is within a general error range.
Because the compounds of the present invention are superior in physical and chemical stability in the form of a crystal, it is advantageous that they are capable of retaining the quality for a long-time, and easily preserved. In addition, advantages such as easy handling during production of various pharmaceutical compositions and bulk drug and decreased production cost can be attained. Therefore, the crystals of the present invention are extremely useful as a medicine.
7.02, 10.96, 11.44, 13.12, 14.14, 16.52, 21.30,
21.80, 22.04, 23.14, 25.56, 26.56, 28.12, 29.34,
10.54, 11.24, 13.16, 19.04, 20.00, 21.24, 21.60,
24.88, 25.26, 26.10, 26.88, 28.32, 28.94, 34.40
22.76, 22.98, 25.70, 26.24, 26.40, 27.76
32.98, 33.64, 34.42, 35.18, 35.62
22.16, 22.78, 23.46, 23.88, 24.52, 25.70, 26.82,
25.52, 26.26, 26.60, 27.16, 28.18, 30.68, 31.04,
19.94, 21.86, 22.52, 23.84, 24.50, 25.32, 25.56,
26.14, 26.42, 30.20, 31.34, 31.86, 33.04, 34.84,
7.58, 12.10, 12.76, 14.64, 15.24, 15.70, 17.24,
10.06, 12.80, 15.10, 15.76, 16.56, 17.10, 17.48,
19.06, 19.94, 20.76, 22.30, 22.90, 24.12, 24.36,
23.90, 25.56, 25.88, 26.66, 27.02, 28.00, 29.46,
7.26, 10.54, 12.84, 13.54, 13.84, 14.60, 15.54,
22.62, 24.48, 25.12, 25.70, 27.92, 28.44, 29.60,
11.72, 13.28, 14.34, 16.62, 21.24, 21.66, 21.78,
22.54, 22.82, 23.68, 24.46, 25.50, 25.78, 26.22,
24.72, 25.14, 25.98, 27.38, 28.46, 30.26, 32.10,
30.92, 31.14, 34.42, 34.92, 36.72, 39.62
7.16, 14.40, 16.72, 20.96, 21.72, 22.14, 22.68,
25.54, 25.92, 27.20, 28.62, 29.92, 30.30, 30.94,
10.48, 11.32, 11.76, 12.08, 16.58, 18.30, 19.68,
20.18, 21.16, 22.28, 22.84, 23.74, 24.62, 25.46,
25.76, 26.18, 26.74, 27.90, 29.64, 32.88, 33.60
23.40, 24.42, 24.88, 25.52, 25.98, 27.24, 28.62
9.30, 13.44, 13.96, 14.52, 14.90, 18.64, 19.90,
8.92, 10.46, 14.82, 15.06, 16.48, 17.98, 18.26,
7.12, 10.72, 12.90, 14.32, 15.20, 16.48, 20.98,
7.22, 13.84, 14.54, 16.16, 16.56, 19.56, 21.02,
The present invention is explained in detail by referring to the following Preparation Examples, which are not to be construed as limitative.
1), 2), 3) and 4) are admixed and filled in a gelatin capsule.
The total amount of 1), 2) and 3) and 30 g of 4) are kneaded with water, vacuum dried and sized. This sized powder is admixed with 14 g of 4) and 1 g of 5) and the mixture is punched with a tableting machine. In this way, 1000 tablets containing 30 mg of the compound of Example 1 per tablet are obtained.
Capsule or tablet can be produced for the compounds of Examples 2 to 95 in the same manner as in Example the above-mentioned Formulation Example 1 or Formulation Example 2.
Human URAT1 full length cDNA was subcloned to expression vector pcDNA3.1 and human URAT1 gene was transfected into human embryonic kidney derived cell line (HEK293 cells) by liposome method using Lipofectamine2000. Simultaneously, HEK293 cells transfected with expression vector pcDNA3.1 alone (hereinafter mock cells) were also produced. HEK293 cells expressing human URAT1 gene or mock cells were selected with geneticin resistance as an index. The functional expression of human URAT1 gene was confirmed by a method similar to the following method, using transport of uric acid labeled with 14C into the cells as an index.
Human URAT1 expressing HEK293 cells or mock cells were cultured in Dulbecco's modified Eagle's MEM medium (high glucose) containing 10% fetal bovine serum, 0.5 mg/mL geneticin sulfate, 100 units/mL penicillin and 100 μg/mL streptomycin under the conditions of 37° C. and 5% CO2 in an incubator. The cells were plated in a 96 well plate (poly-D-Lysine coated) at 1×105 cells/well and the following uric acid transport inhibitory test was performed 24 hr later. This test was performed at room temperature.
After the medium was removed by aspiration from each well, the cells were washed once with Hank's Balanced Salt Solution (HBSS) and preincubated with HBSS (100 mL/well) for 5 min. HBSS was aspirated and an assay buffer (wherein NaCl in the above-mentioned HBSS had been substituted with Na-gluconate) containing various concentrations of the Example compound and a radioactive ligand (uric acid labeled with 14C; final concentration 50 mM) was added to each well at 50 mL/well and an uptake reaction was carried out for 5 min. After the reaction, the cells were washed twice with ice-cold HBSS (150 mL/well), and Microscin ti TM 20 (PerkinElmer) was added at 50 mL/well. The cells were lysed by stirring and the radioactivity of each well was measured in a liquid scintillation counter (TOP COUNT, Packard).
The uric acid transport rate (%) of the Example compound at each concentration was calculated relative to the radioactivity (difference in radioactivity between human URAT1 expressing HEK293 cells and mock cells without addition of Example compound (DMSO addition)) showing URAT1 specific uric acid transport as 100%, and the concentration (IC50) of the Example compound necessary for inhibiting the uric acid transport rate by 50% was determined. The results are shown in Table 23-Table 24. In Table-Table , “+++” means IC50 value of less than 100 nM, “++” means IC50 value of 100 nM to less than 1000 nM, and “+” means IC50 value of 1000 nM to less than 3000 nM.
Human liver microsome (20 mg protein/mL, 5 μL, Xenotech LLC, purchased from Lenexa KS) was suspended in 100 mM potassium phosphate buffer (70 μL, pH 7.4), mixed with a solution (0.5 μL) of the Example compound dissolved in DMSO and preincubated at 37° C. for 5 min. NADPH producing system coenzyme solution (β-nicotinamide adenine dinucleotide phosphate: 5.2 mM, D-glucose-6-phosphate: 13.2 mM, magnesium chloride: 13.2 mM, glucose-6-phosphate dehydrogenase: 1.8 U/mL) 25 μL, and a model substrate (CYP3A4: midazolam 1 mM, CYP2D6: bufuralol 1 mM, CYP2C9: diclofenac 2 mM) 0.5 μL dissolved in DMSO were added to start the reaction. After incubation at 37° C. for 10 min., acetonitrile (200 μL) containing an internal standard substance (propranolol 1 μM) was added and the mixture was centrifuged (room temperature, 3000 rpm, 20 min). The amount of the metabolite produced from each model substrate in the supernatant was measured by high performance liquid chromatography/mass spectrometry (LC/MS/MS) and each CYP enzyme activity was determined. The concentration showing 50% inhibition (IC50) was calculated relative to the enzyme activity without addition of Example compound (DMSO 0.5 μL added) as 100%. The results are shown in Table 25-Table 27.
As is clear from the above-mentioned Experimental Example 1 (uric acid transport inhibitory test using human URAT1 expression cells), the compound of the present invention and a pharmaceutically acceptable salt thereof have a superior inhibitory action on URAT1 activity. As is clear from Experimental Example 2 (CYP inhibitory test), the compound of the present invention and a pharmaceutically acceptable salt thereof have no or extremely low CYP inhibitory action.
They indicate that the compound of the present invention and a pharmaceutically acceptable salt thereof have an effect of strong suppression of reabsorption of uric acid, and very low fear of side effect since they do not substantially inhibit CYP.
Therefore, the compound of the present invention inhibits reabsorption of uric acid by inhibiting URAT1 activity, whereby affords a drug for the prophylaxis or treatment of pathology showing involvement of uric acid, such as hyperuricemia, gouty tophus, acute gouty arthritis, chronic gouty arthritis, gouty kidney, urolithiasis, renal function disorder, coronary artery disease, ischemic heart disease and the like.
Moreover, as the causal disease of the above-mentioned pathology showing involvement of uric acid, complications or diseases highly possibly complicated, for example, gout arthritis, urolithiasis, hypertension or hypertensive complications, hyperlipidemia or hyperlipidemic complications, diabetes or diabetic complications, obesity or obesity complications, decreased uric acid excretion secondary hyperuricemia and the like can be mentioned. A combined use of an agent for the prophylaxis or treatment of these diseases including hyperuricemia and a pharmaceutically effective amount of the compound of the present invention or a pharmaceutically acceptable salt thereof is effective for the prophylaxis or treatment of these diseases. In addition, a combined use of a pharmaceutical agent that increases the blood uric acid level and a pharmaceutically effective amount of the compound of the present invention or a pharmaceutically acceptable salt thereof is effective for suppressing the increase in the blood uric acid level.
The present invention is useful for the prophylaxis or treatment of pathology showing involvement of uric acid, such as hyperuricemia, gouty tophus, acute gouty arthritis, chronic gouty arthritis, gouty kidney, urolithiasis, renal function disorder, coronary artery disease, ischemic heart disease and the like.
This application is based on a patent application No. 2004/344563 filed in Japan and a patent application No. 60/634,223 filed in USA the contents of which are hereby incorporated by reference.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2004-344563 | Nov 2004 | JP | national |
| Number | Date | Country | |
|---|---|---|---|
| 60634223 | Dec 2004 | US |
