Claims
- 1. An isolated protein comprising the amino acid sequence shown in SEQ ID NO:2.
- 2. An isolated protein comprising the amino acid sequence shown in SEQ ID NO:7.
- 3. An isolated peptide comprising an amino acid chain of ten or more consecutive amino acids of the sequence shown in SEQ ID NO:2.
- 4. The isolated peptide according to claim 3, wherein said ten or more consecutive amino acids are from the sequence shown in SEQ ID NO:8.
- 5. The isolated peptide according to claim 3, wherein said ten or more consecutive amino acids are from the sequence shown in SEQ ID NO:9.
- 6. The isolated peptide according to claim 3, wherein said ten or more consecutive amino acids are from the sequence shown in SEQ ID NO:10.
- 7. The isolated peptide according to claim 3, wherein said ten or more consecutive amino acids are from the sequence shown in SEQ ID NO:11.
- 8. The isolated peptide according to claim 3, wherein said ten or more consecutive amino acids are from the sequence shown in SEQ ID NO:12.
- 9. A method for detecting non-A, non-B hepatitis related antibodies in a sample comprising:
- (a) reacting said sample with a non-A, non-B hepatitis related antigen comprising the amino acid sequence shown in SEQ ID NOS:2 or 7-12 or a NANB hepatitis related epitope of a peptide, said NANB hepatitis related epitope comprising an amino acid chain of ten or more consecutive amino acids of SEQ ID NO:2 attached to a solid substrate under conditions which allow the formation of an antigen-antibody complex;
- (b) reacting said product of step (a) with a labelled anti-human IgG or IgM antibody; and
- (c) detecting the presence of said labelled anti-human IgG or IgM antibody bound to said antigen-antibody complex.
- 10. A non-A, non-B hepatitis diagnostic test kit for analyzing samples for the presence of antibodies directed to an antigen comprising the amino acid sequence according to SEQ ID NOS:2 or 7-12 or a NANB hepatitis related epitope of a peptide, said NANB hepatitis related epitome comprising an amino acid chain of ten or more consecutive amino acids of the sequence shown in SEQ ID NO:2 attached to a solid substrate.
Priority Claims (4)
Number |
Date |
Country |
Kind |
2-028191 |
Feb 1990 |
JPX |
|
2-153887 |
Jun 1990 |
JPX |
|
2-335806 |
Nov 1990 |
JPX |
|
3-104010 |
Feb 1991 |
JPX |
|
REFERENCE TO RELATED APPLICATIONS
The present application is a division of U.S. patent application Ser. No. 08/394,326, filed on Feb. 23, 1995, now U.S. Pat. No. 5,766,867 which is a continuation-in-part of U.S. patent application Ser. No. 07/653,090, filed Feb. 8, 1991, now abandoned, which is a CIP U.S. patent application Ser. No. 07/798,226, filed on Nov. 27, 1991, now abandoned which is a CIP and U.S. patent application Ser. No. 07/828,669, filed on Feb. 7, 1992, now abandoned.
Non-Patent Literature Citations (1)
Entry |
Mishiro, S., et al., The Lancet, Dec. 8, 1990, vol. 336, pp. 1400-1403. |
Divisions (1)
|
Number |
Date |
Country |
Parent |
394326 |
Feb 1995 |
|
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
653090 |
Feb 1991 |
|