Patent Grant
8581026
Not applicable.
This invention relates to a new Sesamum indicum L. variety with improved non-dehiscence (IND) appropriate for mechanized harvesting.
Sesame, or Sesamum indicum, is a tropical annual cultivated worldwide for its oil and its nut flavored seeds. The sesame plant grows to a height of about 52-249 cm, and at its leaf axils are found capsules, which contain the sesame seed. Upon maturity in nature, the capsules holding the sesame seeds begin to dry down, the capsules normally split open, and the seeds fall out. Commercially, the harvester tries to recover as much seed as possible from mature capsules. From ancient times through the present, the opening of the capsule has been the major factor in attempting to successfully collect the seed. Harvesting methods, weather, and plant characteristics all contribute to the amount of seed recovered.
The majority of the world's sesame is harvested manually. With manual non-mechanized methods, it is desirable for the sesame seed to fall readily from the plant. Manual harvesting is labor intensive. Efforts to mechanize or partially mechanize harvesting met with limited success.
A breakthrough was accomplished when non-dehiscent (ND) sesame was developed and patented by Derald Ray Langham. ND sesame was found to possess the proper characteristics, which would enable mechanical harvesting without the seed loss disadvantages reported with prior varieties.
U.S. Pat. Nos. 6,100,452; 6,815,576; 6,781,031; 7,148,403; 7,332,652; and 8,003,848 each disclose and claim non-dehiscent (ND) sesame cultivars having various characteristics.
U.S. Pat. Nos. 7,847,149; 7,855,317; 7,964,768; 8,058,503; 8,080,707; and 8,207,397 each disclose and claim improved non-dehiscent (IND) sesame cultivars having various characteristics.
A new sesame variety designated Sesaco 35 (S35) with representative seed having been deposited under ATCC Accession No. PTA-12708. In one aspect, the invention comprises a seed of sesame variety designated S35, a sample of said seed having been deposited under ATCC Accession No. PTA-12708. In another aspect, the invention comprises a sesame plant produced by growing the seed of sesame variety S35, a sample of said seed having been deposited under ATCC Accession No. PTA-12708.
In yet another aspect, the invention comprises plant cells derived from a sesame plant, said plant produced by growing the seed of sesame variety S35, a sample of said seed having been deposited under ATCC Accession No. PTA-12708. The plant cells may be selected, for example, from pollen, tissue culture of regenerable cells, and asexually reproducing cultivars.
In yet another aspect, the invention comprises a sesame plant having all the physiological and morphological characteristics of sesame variety S35, a sample of the seed of said variety having been deposited under ATCC Accession No. PTA-12708.
In another aspect, the invention comprises a sesame plant regenerated from a tissue culture of regenerable cells produced from plant cells derived from sesame variety S35, a sample of said seed having been deposited under ATCC Accession No. PTA-12708, wherein said regenerated sesame plant has all the physiological and morphological characteristics of said sesame variety S35. The plant cells may be derived from S35 seeds or plant cells from tissue from a sesame plant produced by growing the seed of sesame variety S35.
In another aspect, the invention comprises a method of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant and harvesting the resultant sesame seed, wherein said first or second parent sesame plant was produced by growing seed of sesame variety S35, a sample of said seed having been deposited under ATCC Accession No. PTA-12708.
Herein disclosed is a Non-Dehiscent (ND) sesame variety designated Sesaco 35 (S35), which exhibits Improved Non-Dehiscence (IND) and novel characteristics which make it a commercially suitable sesame line. S35 has better whitefly tolerance which leads to a higher yield, and a larger, heavier seed leads to better quality for the market than heretofore described ND and IND sesame varieties. By virtue of having IND, there is less seed loss when left in the field past prime harvest time in adverse weather conditions, for example, rain, fog, dew, and wind. S35 is suitable for mechanical harvesting.
The Improved Non-Dehiscent (IND) classes of sesame, developed by Derald Ray Langham, are ND, but IND sesame also exhibits better adhesion between false membranes and improved placenta attachment. IND sesame holds more seed than prior sesame types, as measured four weeks after a crop is ready for harvest (could have been combined). IND characteristics offer advantages for certain growing applications.
Compared to ND sesame, IND sesame has more seed in the capsules when measured between 4 and 9 weeks after the ideal harvest time.
Without wishing to be bound by one particular theory, it is believed that this increased amount of seed in the capsules may be due to the S35 variety having the ability to better withstand adverse environmental conditions such as inclement or harsh weather. Examples of adverse weather conditions to which S35 has been subjected in this regard are rain, fog, dew, and wind. S35 variety has been tested and meets the criteria of IND.
U.S. Pat. No. 8,080,707 is herein incorporated by reference as if fully set forth herein. This application discloses Improved Non-Dehiscent Sesame. S35 is an example of a variety which resulted from breeding methods described therein.
S35 exhibits improved shatter resistance, acceptable tolerance to common fungal diseases, and a maturity that allows a wide geographical range. Further, S35 exhibits higher yield in geographical locations desirable for sesame planting, and exhibits desirable seed size and seed color. S35 is suitable for planting in areas that have approximately a 21° C. ground temperature when planted in the spring and night temperatures above 5° C. for normal termination. An exemplary desirable geographical area for S35 is from South Texas at the Rio Grande to Central Kansas and from east Texas westward to elevations below 1,000 meters. Other exemplary areas are areas of the United States or of the world, which areas have similar climatic conditions, daylength patterns (similar latitudes) and elevations.
In describing the present invention, it is helpful to be aware of some terminology. Sesame plants have been studied for their response to seasonal and climatic changes and the environment in, which they live during the different phases and stages of growth and development. This type of study, called “phenology” has been documented by the inventor in Langham, D. R. 2007. “Phenology of sesame,” In: J. Janick and A. Whipkey (ed.), Issues in New Crops and New Uses, ASHS Press, Alexandria, Va.
Table I summarizes the phases and stages of sesame, and will be useful in describing the present invention.
ZDAP = days after planting. These numbers are based on S26 in 2004 Uvalde, Texas, under irrigation.
There are several concepts and terms that are used in this document that should be defined. In the initial drydown stage in Table I, the capsules begin to dry and open. This stage ends when 10% of the plants have one or more dry capsules. The late drydown stage ends when the plants are dry enough so that upon harvest, the seed has a moisture content of 6% or less. At this point some of the capsules have been dry for 5 weeks in the example used in Table I, but in other environments for other varieties, the drying can stretch to 7 weeks. The “ideal harvest time” is at the end of the late drying stage. At this point, a combine (also sometimes referred to as a combine harvester, a machine that combines the tasks of harvesting, threshing, and cleaning grain crops) can be used to cut and thresh the plants and separate the seed from the undesired plant material. However, at times, weather may prevent harvest at the ideal time. The plants may have to remain in the field for as much as an additional four weeks, and in some cases even longer. Thus, time t0 corresponds to the ideal harvest time and time t1, which corresponds to the time the grower actually harvests the sesame, is a time later than time t0.
The pedigree method of plant breeding was used to develop S35. Sesame is generally self-pollinated. Crossing is done using standard techniques as delineated in Yermanos, D. M. 1980. “Sesame. Hybridization of crop plants,” Am Soc. Agronomy-Crop Sci. of America, pp. 549-563 and U.S. Pat. No. 6,100,452. Ashri provides an overview of sesame breeding in Ashri, A. (1998). “Sesame breeding,” Plant Breed. Rev. 16:179-228 and Ashri, A. 2007. Sesame (Sesamum indicum L.). In: R. J. Singh, Ed., Genetic Resources, Chromosome Engineering, and Crop Improvement, Vol. 4, Oilseed Crops, p. 231-289, CRC Press, Boca Raton, Fla., USA.
The lineage of S35 is presented in
104 (2) was a line obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with the designator SF084. The Sesamum Foundation obtained it from Maximo Rodriguez in 1961. He had collected it from Mexico where it was known as Instituto 8. Instituto 8 was a selection from G53.48, a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, 104 carried the identifier 0084. In 1983, a selection of this line became Sesaco 2 (S02).
192 (3) was a line obtained from the M. L. Kinman in 1980 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. The line was originally T61429-B-4-1-3 from the Kinman USDA sesame program, College Station, Tex., which had been in cold storage at Ft. Collins, Colo. In 1997, the line was transferred to the NPGS, Griffin, Ga., and given the identifier PI599462. Within Sesaco, 192 first carried the identifier 1479 and then was changed to X191 and X193. In 1985, a selection from X193 became Sesaco 3 (S03) and a selection of X191 became Sesaco 7 (S07)
R234 (4) was a named variety obtained from D. M. Yermanos in 1978 from his sesame program at the University of California at Riverside. It was first planted in the Kamman nursery (Wellton, Ariz.) in 1978. Within Sesaco, it carried the identifier 0544 and then changed to T234.
G8 (5) was a line obtained from D. G. Langham in 1977 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. It was a selection from the cultivar ‘Guacara’, which D. G. Langham developed in Venezuela in the 1950s. Guacara was an initial selection from a cross that later produced one of the major varieties in Venezuela—Aceitera. Within Sesaco, G8 first carried the identifier X011 and was later changed to TG8.
958 (6) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF411. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. Within Sesaco, G958-1 carried the identifier 0411 and later changed to T958.
982 (7) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF477 and was named G53.98-2. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. G53.98-2 was a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, 982 carried the identifier 0477 and then changed to T982.
G53.80-1 (8) was a line obtained from the Sesamum Foundation in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with a designator of SF471. The Sesamum Foundation obtained it from John Martin in 1962 who had obtained it from the D. G. Langham breeding program in Venezuela. G53.80-1 was a cross made by D. G. Langham in 1953 in Guacara, Venezuela. Within Sesaco, G53.80-1 carried the identifier 0471.
191 (9) was a selection from 192, which was a line obtained from the M. L. Kinman in 1980 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. The line was originally T61429-B-4-1-3 from the Kinman USDA sesame program, College Station, Tex., which had been in cold storage at Ft. Collins, Colo. In 1997, the line was transferred to the NPGS, Griffin, Ga. and given the identifier PI599462. Within Sesaco, 192 first carried the identifier 1479 and then was changed to X191 and X193. In 1985, a selection from X193 became Sesaco 3 (S03) and a selection of X191 became Sesaco 7 (S07).
MEL (10) was a line obtained from Mel Tiezen in 1978 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. Mr. Tiezen obtained it from a farmer in Mexico. Within Sesaco, MEL first carried the identifier 0543 and was then changed to TMEL.
V52 (11) was a cultivar designated as SF075 obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) collection in 1977 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. The Sesamum Foundation obtained it from B. Mazzani (Maracay, Venezuela) in 1960. Originally, it was a cultivar known as Venezuela 52 developed by D. G. Langham in the 1940s. Within Sesaco, V52 first carried the identifier 0075 and was later changed to TV52.
SOMALIA (12) was a line obtained from the NGPS (PI210687) in 1979 and first planted in Kamman nursery (Wellton, Ariz.) in 1979. The NGPS obtained it from the Administrazione Fiduciaria Italiana della Somalia, Mogadishu, Somalia. Within Sesaco, it carried the identifier 0730.
118 (13) was a line obtained from the NGPS (PI425944) in 1979 and first planted in Kamman nursery (Wellton, Ariz.) in 1979. The NGPS obtained it in 1978 from P. F. Knowles, University of California, Davis, Calif., who collected it in Pakistan. Within Sesaco, it carried the identifier 1118, which was changed to X118 and then to T118.
MAXIMO (14) was a line obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. The Sesamum Foundation obtained it from Maximo Rodriguez in 1961. He had collected it from Mexico where it was known as Instituto Regional Canasta. Within Sesaco, it carried the identifier 0116 and then changed to TMAX.
R234 TALL (15) was an outcross found in a population of R234 (4) in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier X026.
195 (16) was an outcross selected in plot MN4584 in a population of 192 (3) in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it carried the identifier E0690 and then changed to X195.
701 (17) was a line obtained from the NGPS (PI292145) in 1979 and first planted in Woods nursery (Wellton, Ariz.) in 1981. The NGPS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from the Indian subcontinent. Within Sesaco, it carried the identifier 0701 and then changed to X701. In 1984, a selection from X701 became Sesaco 5 (505).
111X (18) was an outcross in the 111 (1) plot BT0458 in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier E0745 and was later changed to T111X.
804 (19) was a cross made by Sesaco between G8 (5) and 111X (18) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it has carried the identifier F804; in 1988, a selection of this line became Sesaco 11 (S11).
F822 (20) was a cross made by Sesaco between 111X (18) and 192 (3) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F822.
700 (21) was a line obtained from the NPGS (PI292144) in 1979 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. NPGS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from India and Pakistan. Within Sesaco, 700 first carried the identifier 0700 and was later changed to T700.
F820 (22) was a cross made by Sesaco between 111X (18) and 104 (2) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F820.
F853 (23) was a cross made by Sesaco between 104 (2) and 192 (3) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier F853.
96B (24) was an outcross in the 191 (9) in plot 4637 in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it carried the identifier E0690, which later became X196B and was later changed to T96B.
S11 (25) was a cross made by Sesaco between G8 (5) and 111X (18) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it has carried the identifier F804. In 1988, a selection of this line became Sesaco 11.
B043 (26) was a cross made by Sesaco between G8 (5) and MEL (10) in the Kamman nursery (Yuma, Ariz.) in 1978. Within Sesaco, it carried the identifier B043.
G54 (27) was a line obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) in 1977 and first planted in the Kamman nursery (Wellton, Ariz.) in 1978. It was obtained with the designator SF408. The Sesamum Foundation obtained it from John Martin in 1962. This line was given to Mr. Martin by D. G. Langham. G54 was a selection from G53.48, a cross made by D. G. Langham in 1954 in Guacara, Venezuela. Within Sesaco, G54 carried the identifier 0408 and was then changed to TG54.
578 (28) was a cross made by Sesaco between F820 (22) and F853 (23) in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it first carried the identifier G8578 and was later changed to T578.
888 (29) was a cross made by Sesaco between 192 (3) and V52 (11) in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, 888 first carried the identifier F888 and was later changed to T888.
H6778 (30) was a cross made by Sesaco between SOMALIA (12) and 118 (13) in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6778.
193 (31) was a selection from 192, which was a line obtained from the M. L. Kinman in 1980 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. The line was originally T61429-B-4-1-3 from the Kinman USDA sesame program, College Station, Tex., which had been in cold storage at Ft. Collins, Colo. In 1997, the line was transferred to the NPGS, Griffin, Ga. and given the identifier PI599462. Within Sesaco, 192 first carried the identifier 1479 and then was changed to X191 (single capsule) and X193 (triple capsule). In 1985, a selection from X193 became Sesaco 3 (S03) and a selection of X191 became Sesaco 7 (S07).
076 (32) was a cross made by Sesaco between MAXIMO (14) and R234 TALL (15) in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier C076 and then changed to T076.
045 (33) was a cross made by Sesaco between G8 (5) and 958 (6) in the Kamman nursery (Wellton, Ariz.) in 1978. Within Sesaco, it carried the identifier B045 and then changed to T045.
036 (34) was a cross made by Sesaco between 982 (7) and G53.80-1 (8) in the Kamman nursery (Wellton, Ariz.) in 1979. Within Sesaco, it carried the identifier C036 and then X036. In 1984, a selection from X036 became Sesaco 6 (S06).
H6562 (35) was a cross made by Sesaco between 195 (16) and 701 (17) in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6562.
702 (36) was a line obtained from the NGPS (PI292146) in 1979 and first planted in Woods nursery (Wellton, Ariz.) in 1981. The NGPS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from the Indian subcontinent. Within Sesaco, it has carried the identifier 0702 and then changed to X702. When the selection was made it carried the designator X702C and later changed to 720. In 1986, a selection from 72C became Sesaco 12 (S12).
031 (37) was a cross made by Sesaco between 578 (28) and 118 (13) in the Ramsey nursery (Roll, Ariz.) in 1984. Within Sesaco, it carried the identifier H0031 and then changed to T031.
H6432 (38) was a cross made by Sesaco between 193 (31) and 076 (32) in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6432.
H6785 (39) was a cross made by Sesaco between 045 (33) and 036 (34) in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it carried the identifier H6785.
562 (40) was a cross made by Sesaco between F822 (20) and 700 (21) in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it first carried the identifier G8562 and was later changed to T562.
K0338 (41) was a cross made by Sesaco between 804 (19) and 96B (24) in the Hancock nursery (Wellton, Ariz.) in 1986. Within Sesaco, it carried the identifier K0338.
C063 (42) was a cross made by Sesaco between B043 (26) and G54 (27) in the Kamman nursery (Yuma, Ariz.) in 1979. Within Sesaco, it carried the identifier C063.
88F (43) was a cross between S11 (25) and 888 (29) made by Sesaco in the Sharp nursery (Roll, Ariz.) in 1988. Within Sesaco, it carried the identifier LCE01 and then changed to X88F and then T88F.
J3208 (44) was a cross made by Sesaco between H6778 (30) and H6432 (38) in the Hancock nursery (Wellton, Ariz.) in 1985. Within Sesaco, it carried the identifier J3208.
J3222 (45) was a cross made by Sesaco between H6785 (39) and H6562 (35) in the Hancock nursery (Wellton, Ariz.) in 1982. Within Sesaco, it carried the identifier J3222.
72A (46) was a selection from 0702, which was a line obtained from the NGPS (PI292146) in 1979 and first planted in Woods nursery (Wellton, Ariz.) in 1981. The NGPS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from the Indian subcontinent. Within Sesaco, it has carried the identifier 0702 and then changed to X702. When the selection was made it carried the designator X702A and later changed to 72A.
K3255 (47) was a cross made by Sesaco between J3208 (44) and J3222 (45) in the Hancock nursery (Wellton, Ariz.) in 1986. Within Sesaco, it carried the identifier K3255.
56B (48) was a cross made by Sesaco between 804 (19) and 562 (40) in the Wright nursery (Tacna, Ariz.) in 1987. Within Sesaco, it first carried the identifier KAN00 and was later changed to X56B and then to T56B.
ZSA (49) was a cross made by Sesaco between K0338 (41) and S11 (25) in the Yuma greenhouse (Yuma, Ariz.) in 1986. Within Sesaco, it first carried the identifier KAC22 and was later changed to XZSA and then to TZSA.
233 (50) was a cross made by Sesaco between C063 (42) and 193 (31) in the Hancock nursery (Wellton, Ariz.) in 1984. Within Sesaco, it first carried the identifier H6233 and was later changed to T233.
S16 (51) was a cross made by Sesaco between K3255 (47) and S11 (25) in the Wright nursery (Roll, Ariz.) in 1987. Within Sesaco, it carried the identifier KAP11 and then changed to XFXA. In 1991, a selection from XFXA became Sesaco 16 (S16).
S17 (52) was a cross between S11 (25) and 72A (46) made by Sesaco in the Wright nursery (Roll, Ariz.) in 1987. Within Sesaco, it carried the identifier KAN22 and then changed to X7AB. In 1992, a selection from X7AB became Sesaco 17 (S17).
FXA (53) was a cross made by Sesaco between K3255 (47) and S11 (25) in the Wright nursery (Roll, Ariz.) in 1987. Within Sesaco, it carried the identifier KAP11 and then changed to XFXA. In 1991, a selection from XFXA became Sesaco 16 (S16).
13C (54) was a cross made by Sesaco between 56B (48) and 031 (37) in the Sharp nursery (Tacna, Ariz.) in 1990. Within Sesaco it first carried the identifier PF270 and later was changed to X13C and then T13C.
SAA (55) was a cross made by Sesaco between ZSA (49) and 233 (60) in the Sharp nursery (Roll, Ariz.) in 1989. Within Sesaco, it has carried the identifier PE046 and later changed to XSAA and then to TSAA.
BI791 (56) was a cross between 88F (43) and S16 (51) made by Sesaco in the Gilleland nursery (Uvalde, Tex.) in 1992. Within Sesaco, it carried the identifier BI791.
13A (57) was a cross between 031 (37) and FXA (53) made by Sesaco in the Sharp nursery (Roll, Ariz.) in 1990. Within Sesaco, it carried the identifier PE242 and later changed to X13A and then to T13A.
3CEd (58) was a cross between 13A (57) and 13C (54) made by Sesaco in the Gilleland nursery (Uvalde, Tex.) in 1995. Within Sesaco, it carried the identifier DT354) and later changed to X3CE and then to T3CEd.
13H (59) was a cross made by Sesaco between SAA (55) and 031 (37) in the Gilleland nursery (Uvalde, Tex.) in 1994. Within Sesaco, it carried the identifier CM413 and later changed to X13H and then to T13H.
3HR (60) was a cross made by Sesaco between 3CEd (58) and 13H (59) in the Gilleland nursery (Uvalde, Tex.) in 1999. Within Sesaco, it carried the identifier HG970 and later changed to X3HR.
S27 (61) S27 (35) was a cross between BI791 (56) and S17 (52) made by Sesaco in the Friesenhahn nursery (Knippa, Tex.) in 1994. Within Sesaco, it carried the identifier CM586 and later changed to X88K.
S35 (62) was obtained with the following method. A cross between 3HR (60) and S27 (61) was made in the Gilleland nursery (Uvalde, Tex.) in Year 1 (hereinafter “Year” is abbreviated as “YR”) and designated AV341.
The resulting seed of AV341, designated V341 was planted in a plot 3663 in YR2. Five plants were selected based having a lot of capsules down the row, quick drying phase, a capsule zone and height of the first capsule better than the S27 parent, and branches on branches.
The seed (3897) from one of the plants was planted in a plot 3870 in YR3. Four individual plants were selected based a capsule zone and height of the first capsule better than the S27 parent, and tight branching.
The designator was changed to X27U. The seed (1785) from one of the plants was planted in plot 3830 in YR4. A bulk of 5 plants was selected based on having a good yield, being low, having a wide row, and tight branches.
The seed (0842) from the plants was planted in strip plot W500 in YR4-5 in a winter nursery. The plot was rogued and all of the plants were harvested based on ability to do very well in low moisture compared to the other lines under the same conditions.
The seed (27UCR) from the plants was planted in a farmer field in YR5 for final verification of weather shatter resistance, lodging tolerance, combinability, and yield. The line was uniform and repeated on having good weather shatter resistance and excellent lodging tolerance. Part of the strip was harvested for an increase and part was left for a combine test. The line combined well with no issues. The designator was changed to Sesaco 35. The variety was released to farmers in YR6.
Along with breeding programs for sesame, tissue culture of sesame is currently being practiced in such areas of the world as Korea, Japan, China, India, Sri Lanka and the United States. One of ordinary skill in the art may utilize sesame plants grown from tissue culture as parental lines in the production of non-dehiscent sesame. Further IND sesame may be propagated through tissue culture methods. By means well known in the art, sesame plants can be regenerated from tissue culture having all the physiological and morphological characteristics of the source plant.
The present invention includes the seed of sesame variety S35 deposited under ATCC Accession No. PTA-12708; a sesame plant or parts thereof produced by growing the seed deposited under ATCC Accession No. PTA-12708; any sesame plant having all the physiological and morphological characteristics of sesame variety S35; any sesame plant having all the physiological and morphological characteristics of a sesame plant produced by growing the seed deposited under ATCC Accession No. PTA-12708. The present invention also includes a tissue culture of regenerable cells produced from the seed having been deposited under ATCC Accession No. PTA-12708 or a tissue culture of regenerable cells from sesame variety S35 or a part thereof produced by growing the seed of sesame variety S35 having been deposited under ATCC Accession No. PTA-12708. A sesame plant regenerated from a tissue culture of regenerable cells produced from the seed having been deposited under ATCC Accession No. PTA-12708 or from sesame variety S35, wherein the regenerated sesame plant has all the physiological and morphological characteristics of sesame variety S35 is also contemplated by the present invention. Methods of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, wherein the first or second parent sesame plant was produced by seed having been deposited under ATCC Patent Deposit Designation No. PTA-12708 is part of the present invention.
Unless otherwise stated, as used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like. Further, unless otherwise stated, as used herein, the term progeny includes plants derived from plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like.
Sesame cultivar S35 has been tested experimentally over several years under various growing conditions ranging from South Texas to Southern Oklahoma. Sesame cultivar S35 has shown uniformity and stability within the limits of environmental influence for the characters listed in Table II below. Table II provides the name, definition, and rating scale of each character as well as the method by which the character is measured. Under the rating section, the rating for S35 is presented in bold text. Additionally, the distribution of the character in Sesaco's sesame development program is indicated under the rating section. Sesaco uses slightly different character specifications from “Descriptors for sesame,” AGP:IBPGR/80/71, IBPGR Secretariat, Rome, (1981) and from the form “Sesame (Sesamum indicum),” U.S. Department of Agriculture Plant Variety Protection Office, Beltsville, Maryland. The descriptors in those documents were developed in the early 1980s and have not been updated to incorporate new concepts in sesame data collection.
Table II provides characteristics of S35 for forty-five (45) traits. Numerical ratings and values reported in this table were experimentally determined for S35 with prior sesame varieties in side by side replicated trials. Actual numerical values and ratings for a given variety will vary according to the environment, and the values and ratings provided in Table II were obtained in the environment specified in the parenthetical following the S35 rating. If “NT” is indicated, it indicates that trait was not tested. Table V provides a direct comparison between the new S35 variety and the prior varieties thus demonstrating the relative differences between the varieties in the side by side trials.
Macrophomina
phaseoli, and Phytophthora
parasitica. Between 1988 and the present,
Macrophomina; too much water can
FUSARIUM WILT (F.
oxysporum)
Fusarium wilt
PHYTOPHTHORA
FUSARIUM WILT (Character No. 35)
parasitica)
Phytophthora stem rot
FUSARIUM WILT (Character No. 35)
phaseoli)
FUSARIUM WILT (Character No. 35)
sesami)
argentifolii)
tabaci) has been observed in nurseries
persicae)
Heliothis has affected nurseries or
aUvalde nursery planted north of Uvalde, Texas (latitude 29°22′ north, longitude 99°47′ west, 226 m elev) in middle to late May to early June from 1988 to the present; mean rainfall is 608 mm annually with a mean of 253 mm during the growing season; temperatures range from an average low of 3° C. and an average high of 17° C. in January to an average low of 22° C. and an average high of 37° C. in July. The nursery was planted on 96 cm beds from 1988 to 1997 and on 76 cm beds from 1998 to the present. The nursery was pre-irrigated and has had 2-3 post-plant irrigations depending on rainfall. The fertility has varied from 30-60 units of nitrogen.
bLorenzo nursery planted southeast of Lubbock, Texas (latitude 33°40′ north, longitude 101°49′ west, 1000 m elev) in mid-June from 2004 to the present; mean rainfall is 483 mm annually with a mean of 320 mm during the growing season; temperatures range from an average low of −4° C. and an average high of 11° C. in January to an average low of 20° C. and an average high of 33° C. in July. The nursery was planted on 101 cm beds. The nursery was rainfed. The fertility was 30 units of nitrogen.
cPuerto Rico nursery planted east of Ponce, Puerto Rico (latitude 18°15′ north, longitude 66°30′ west, 50 m elev) in December; mean rainfall is 963 mm annually with a mean of 0 mm during the growing season; temperatures range from an average low of 19° C. and an average high of 30° C. in January to an average low of 23° C. and an average high of 33° C. in July. The nursery was planted on 101 cm beds. The nursery had drip irrigation. The fertility was 60 units of nitrogen.
In developing sesame varieties for the United States, there are eight characters that are desirable for successful crops: SHAKER SHATTER RESISTANCE (Character No. 23), IMPROVED NON-DEHISCENT VISUAL RATING (Character No. 26), COMPOSITE KILL TOLERANCE (Character No. 35), DAYS TO PHYSIOLOGICAL MATURITY (Character No. 30), YIELD AT DRYDOWN (Character 11), SEED COLOR (Character No. 32), SEED WEIGHT−100 SEEDS FROM THE ENTIRE PLANT (Character No. 33), and SEED OIL CONTENT (Character No. 45). The first four characters contribute to YIELD AT DRYDOWN, which is the most important economic factor normally considered by a farmer in the selection of a variety. The last three characters determine the market value of the seed.
SHAKER SHATTER RESISTANCE and IMPROVED NON-DEHISCENT VISUAL RATING determine how well the plants will retain the seed while they are drying down in adverse weather.
COMPOSITE KILL TOLERANCE determines whether the plants can finish their cycle and have the optimum seed fill.
DAYS TO PHYSIOLOGICAL MATURITY determines how far north and to which elevation the varieties can be grown.
In improving the characters, the YIELD AT DRYDOWN has to be comparable to or better than current varieties, or provide a beneficial improvement for a particular geographical or market niche.
In the United States and Europe, the SEED COLOR is important since over 95% of the market requires white or buff seed. There are limited markets for gold and black seed in the Far East. All other colors can only be used in the oil market.
SEED WEIGHT−100 SEEDS FROM THE ENTIRE PLANT determines the market for the seed. Lack of Composite kill Tolerance can reduce SEED WEIGHT−100 SEEDS FROM THE ENTIRE PLANT. In parts of the United States where there is little rain in dry years, the lack of moisture can reduce the SEED WEIGHT−100 SEEDS FROM THE ENTIRE PLANT.
SEED OIL CONTENT determines the market for the seed. Lack of Composite kill Tolerance can reduce SEED OIL CONTENT. Low fertility in terms of nitrogen raises SEED OIL CONTENT and high fertility lowers SEED OIL CONTENT.
There are other characters important in developing commercial sesame varieties explained in Langham, D. R. and T. Wiemers, 2002. “Progress in mechanizing sesame in the US through breeding”, In: J. Janick and A. Whipkey (ed.), Trends in new crops and new uses, ASHS Press, Alexandria, Va. BRANCHING STYLE (Character No. 1), HEIGHT OF PLANT (Character No. 5) and HEIGHT OF FIRST CAPSULE (Character No. 6) are important in combining. CAPSULE ZONE LENGTH (Character No. 7), NUMBER OF CAPSULE NODES (Character No. 8), AVERAGE INTERNODE LENGTH WITHIN CAPSULE ZONE (Character No. 9), and SEED WEIGHT PER CAPSULE (Character No. 18) are important in creating potential YIELD AT DRYDOWN (Character No. 10). LEAF DIMENSIONS (Characters No. 12, 13, 14, and 15) are important in determining optimum populations.
NUMBER OF CAPSULES PER LEAF AXIL (Character No. 2), NUMBER OF CARPELS PER CAPSULE (Character No. 16), CAPSULE LENGTH (Character No. 17), CAPSULE WEIGHT PER CAPSULE (Character No. 19), and CAPSULE WEIGHT PER CM OF CAPSULE (Character No. 20) are important in breeding for VISUAL SEED RETENTION (Character No. 21) and IMPROVED NON-DEHISCENT VISUAL RATING (Character No. 25), which lead to testing for SHAKER SHATTER RESISTANCE (Character No. 22) and determining the CAPSULE SHATTERING TYPE (Character No. 23), NON-DEHISCENT TEST (Character 24) and IMPROVED NON-DEHISCENT TEST (Character No. 26).
DAYS TO FLOWERING (Character No. 27), DAYS TO FLOWER TERMINATION (Character No. 28), DAYS TO PHYSIOLOGICAL MATURITY (Character No. 29) and MATURITY CLASS (Character No. 3) are highly correlated and important in determining the phenology and geographical range for the variety.
DAYS TO DIRECT HARVEST (Character No. 30) is important in that once the plants reach physiological maturity there is no weather event that will increase yield and many weather events that may substantially lower the yield. A shorter drying phase increases yield. PLANT PHENOTYPE (Character No. 4) is a summary character of characters 1, 2, and 3 that allows an overall visualization of the line.
TOLERANCE TO DROUGHT (Character No. 11) may reduce yield and seed weight. Even though there was a drought in the growing areas in 2006, there has not been a drought in nurseries planted since 2000 because of irrigation. LODGING TOLERANCE (Character No. 31) is important in years when there are high winds in the growing areas. The tolerance characters (Characters No. 35, 36, 37, 38, 39, 40, 41, 42, and 43) are important in reducing the losses from diseases and pests.
Over the past 35 years, Sesaco has tested 2,990 introductions from all over the world. Commercial samples have been obtained from China, India, Sudan, Ethiopia, Burkina Faso, Nigeria, Mozambique, Pakistan, Myanmar, Bangladesh, Vietnam, Egypt, Mexico, Guatemala, Nicaragua, Venezuela, Thailand, Turkey, Upper Volta, Uganda, Mali, Kenya, Indonesia, Sri Lanka, Afghanistan, Philippines, Colombia, Ivory Coast, Gambia, Somalia, Eritrea, Paraguay, Bolivia, and El Salvador. Additional research seed has been received from the commercial countries and from many other countries such as Australia, Iraq, Iran, Japan, Russia, Jordan, Yemen, Syria, Morocco, Saudi Arabia, Angola, Argentina, Peru, Brazil, Cambodia, Laos, Sri Lanka, Ghana, Gabon, Greece, Italy, South Korea, Libya, Nepal, Zaire, England and Tanzania. Research seed received from one country may have originated from another unspecified country. All of the commercial and research introductions have CAPSULE SHATTERING TYPE (Character No. 23) of shattering, “SHA”.
Using selected characters from Table II, Table III provides a character differentiation between S35 and name cultivars from all over the world.
a SHA = shattering; SSH = semi-shattering; ID = indehiscent; SR = shatter resistant; XX = not non-dehiscent according to the teachings of U.S. Pat. No. 6,100,452; ND = non-dehiscent according to the teachings of U.S. Pat. No. 6,100,452; IND = improved non-dehiscent according to the teachings of U.S. Pat. No. 8,080,707, py/py = presence of homozygous pygmy alleles; PY/PY = absence of pygmy alleles according to the teachings of U.S. Patent Publication No. 2011/0271359; U = unbranched; B = branched
As a comparison to the S35 variety, there are 3 other branched, Improved Non-dehiscent (IND) sesame cultivars, S27 is disclosed in U.S. Pat. No. 7,964,768; S32 is disclosed in U.S. Pat. No. 7,855,317; and S33 is disclosed in U.S. Pat. No. 8,207,397. S27, and S32, and S33 are stable, commercially suitable sesame lines providing improved non-dehiscence.
Table III differentiates S35 from all other cultivars and varieties except S27, S32, and S33. Table IV provides additional data that separates S35 from S27, S32, and S33.
In comparison to S27, it can be seen in the above table that S35 has a shorter internode length, a higher 2011 Uvalde yield, a comparable 2011 Lorenzo yield, better composite kill tolerance, better bacterial black rot tolerance, and better whitefly tolerance. In comparison to S32, it can be seen in the above table that S35 has a shorter internode length, a higher 2011 Uvalde yield, a higher 2011 Lorenzo yield, better composite kill tolerance, comparable black rot tolerance, and better whitefly tolerance. In comparison to S33, it can be seen in the above table that S35 has a shorter internode length, a higher 2011 Uvalde yield, a higher 2011 Lorenzo yield, comparable composite kill tolerance, higher black rot tolerance, and better whitefly tolerance.
Table V shows all the characters from Table II for S35 and varieties S26, S30, S32, S33, and S34. The table is in terms of all of the characters listed in Table II. The major differences in Table V are indicated in the “Dif” column by a “C” for commercially important differences and an “M” for morphological differences.
Phytophthora Stem Rot
phaseoli)
sesami)
argentifolii)
persica)
aB = true branches; U = uniculm (no true branches); UV = Uvalde nursery; M = medium maturity class of 95-104 days; B1M = phenotype of true branches, single capsules per leaf axil, and medium maturity class of 95-104 days; U1M = phenotype of uniculm, single capsules per leaf axil, and medium maturity class of 95-104 days; LO = Lorenzo nursery; NT = not tested; W = weather visual seed retention >75%; SR = shatter resistant; ND = non-dehiscent; ZZ = not improved non-dehiscent; IND = improved non-dehiscent; BF = buff color; and NEC = no economic damage - not enough disease or insects to do ratings; PY/PY = absence of pygmy alleles.
As stated earlier, in developing sesame varieties for the United States, there are eight important characters: SHAKER SHATTER RESISTANCE (Character No. 22), IMPROVED NON-DEHISCENT VISUAL RATING (Character No. 25), COMPOSITE KILL TOLERANCE (Character No. 34), DAYS TO PHYSIOLOGICAL MATURITY (Character No. 29), YIELD AT DRYDOWN (Character No. 10), SEED COLOR (Character No. 32), SEED WEIGHT−100 SEEDS FROM ENTIRE PLANT (Character No. 33), and SEED OIL CONTENT (Character No. 45). These characters will be discussed first comparing S35 to Sesaco varieties (S26, S30, S32, S33, and S34, which are the current varieties used in the United States). The data is based on planting the varieties side by side with five replications in Uvalde and two in Lorenzo.
S35 has 71.1% SHAKER SHATTER RESISTANCE, which is above the 65% threshold established in U.S. Pat. No. 6,100,452 to qualify S35 as a non-dehiscent variety.
S35 exhibited an IMPROVED NON-DEHISCENT VISUAL RATING rating of 7.4 (data from both Uvalde and Lorenzo nurseries, 2011) which is above the 7.0 threshold established in U.S. Pat. No. 8,080,707 to qualify S35 as an improved non-dehiscent variety.
S35 has a rating of 7.4 for COMPOSITE KILL TOLERANCE (data from Uvalde nursery, 2011) and is an improvement over most of the other patented commercial varieties. Any rating above 5.67 indicates that over 90% of the plants produced good seed to the top of the plant.
The mean DAYS TO PHYSIOLOGICAL MATURITY for S35 is 97, which allows it to be planted in all of the current sesame growing areas. Care should be taken in areas where early freezes may occur to plant S35 in at a time where it will physiologically mature prior to expected freezes.
The yield of S35 is expected to be slightly better or better than that of prior varieties under typical growing conditions and was found to be comparable under less than ideal conditions used for testing as reported in
The SEED COLOR (Character No. 32) of S35 is buff, which is suitable for most of the U.S. and world markets.
S35 seed is heavier than the other patented varieties (0.302 grams per hundred seeds), and thus meets most commercial specifications in the ingredient and natural topping markets for sesame seed, which (in the United States) often set a threshold value of 0.30 grams per hundred seed weight.
S35 exhibited oil contents of 54.1% and 51.7% (data from Uvalde 2010 and 2011) and thus is suitable for use as a source of sesame oil for the commercial oil market.
S35 has demonstrated adequate tolerance to whiteflies. While prior variety S26 has been used as a comparative standard for whitefly tolerance, S35 (with a rating of 5.7) had comparable tolerance than S26 in testing conducted in Puerto Rico.
S35, with a rating of 7.0, exhibits better tolerance when these conditions are present than most previously described varieties.
On Mar. 27, 2012, a deposit of at least 2500 seeds of sesame plant S35 was made by Sesaco Corporation under the provisions of the Budapest Treaty with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209, and the deposit was given ATCC Accession No. PTA-12708. This deposit will be maintained in the ATCC depository for a period of 30 years or 5 years after the last request or for the enforceable life of the patent, whichever is longer. Should the seeds from the sesame line S35 deposited with the American Type Culture Collection become non-viable, the deposit will be replaced by Sesaco Corporation upon request.
The foregoing invention has been described in some detail by way of illustration and characters for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications may be practiced within the scope of the invention as limited only by the scope of the appended claims.
This application claims the benefit of U.S. Provisional Application No. 61/791,110 filed Mar. 15, 2013.
| Number | Name | Date | Kind |
|---|---|---|---|
| 6100452 | Langham | Aug 2000 | A |
| 6781031 | Langham | Aug 2004 | B2 |
| 6815576 | Langham | Nov 2004 | B2 |
| 7148403 | Langham | Dec 2006 | B2 |
| 7332652 | Langham | Feb 2008 | B2 |
| 7847149 | Langham | Dec 2010 | B2 |
| 7855317 | Langham | Dec 2010 | B2 |
| 7964768 | Langham | Jun 2011 | B2 |
| 8003848 | Langham | Aug 2011 | B2 |
| 8058503 | Langham | Nov 2011 | B1 |
| 8080707 | Langham | Dec 2011 | B2 |
| 8207397 | Langham | Jun 2012 | B1 |
| 20060230472 | Langham | Oct 2006 | A1 |
| Number | Date | Country |
|---|---|---|
| WO9915681 | Apr 1999 | WO |
| WO0013488 | Mar 2000 | WO |
| Entry |
|---|
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| Number | Date | Country | |
|---|---|---|---|
| 61791110 | Mar 2013 | US |
