Patent Application
20110082109
The present invention relates to pharmaceuticals, particularly to novel acyl guanidine derivatives which possess inhibitory effects on Na+/H+ exchanger type 3 (hereinafter sometimes referred to as NHE3) and can be orally administered, methods of producing thereof, synthetic intermediates thereof and pharmaceutical compositions comprising the acyl guanidine derivatives.
Na+/H+ exchanger (NHE) is a transporter protein having 12-transmembrane domain which exists on the cell membrane and nine isoforms (NHE 1/SLC9A1˜NHE8/SLC9A9) have been identified (Malo M E, Fliegel L. Can J. Physiol. Pharmacol. 2006; 84 (11); 1081-95). In its C-terminal end domain which is located inside the cell, there are binding domains for variety of factors which are involved in intracellular signal regulations and it is believed that it plays a role in regulating cellular functions by interacting with such factors (Rhysiol. Review 2007, v 87, pp 825-872). NHE is a very important protein, since it is involved in maintaining intracellular pH or moisture and regulating cell proliferation through exchange transport of H+ ions to the extracellular region by using concentration gradients, as driving force, which are generated between the intracellular region and the extracellular region.
NHE3 is highly-expressed in renal tubule and gastrointestinal tract and in particular, plays an important role in regulating Na concentration and pH in the body fluid (Bookstein C, DePaoli A M, Xie Y, Niu P, Musch M W, Rao M C, Chang E B. J Clin Invest. 1994; 93(1): 106-13). It has been reported that proteinuria and loose stool occur in NHE3 knockout mice (Schultheis P J, Clarke L L, Meneton P, Miller M L, Soleimani M, Gawenis L R, Riddle T M, Duffy J J, Doetschman T, Wang T, Giebisch G, Aronson P S, Lorenz J N, Shull G E. Nat. Genet. 1998; 19(3): 282-5) and thus its link to protein reabsorption and regulation of the amount of water in stool have been functionally demonstrated.
In recent years, involvements of NHE3 in pathological conditions such as diabetic nephropathy and metabolic syndrome-related nephropathy has been reported (Klisic J, Nief V, Reyes L, Ambuhl P M. Nephron Physiol. 2006; 102(2): 27-35). Glomerular hyperfiltration occurs in an early stage of these pathological conditions and results in edema or hypertension through enhancement of Na+ reabsorption. It is NHE3 that plays an important role in such an event. It has been reported that NHE3 expression is enhanced by albumin or glucose load in vitro (Stevens V A, Saad S, Poronnik P, Fenton-Lee C A, Polhill T S, Pollock C A. Nephrol Dial Transplant. 2008; 23(6): 1834-43) and thus it has been also believed that NHE3 expression is increased in kidney diseases such as diabetic nephropathy and hypertension, which leads to worsen early symptoms.
NHE inhibitors have long been developed and clinical trials have been conducted to validate whether such inhibitors can be used as pharmaceuticals. It has been demonstrated that Cariporide which is a selective inhibitor against NHE1 is effective for myocardial ischemic injury and it is assumed that Cariporide inhibits progression of myocardial damage by inhibiting H+ increase occurred during ischemic reperfusion and enhancement of Na+ excretion associated with the H+ increase. In addition, it has been reported that 53226 which has been reported to selectively inhibit NHE3 shows an ameliorating effect on renal ischemia and reperfusion injury (Hropot M, Juretschke H P, Langer K H, Schwark J R. Kidney Int. 2001; 60(6): 2283-2289).
As NHE3 inhibitors, derivatives having a diacylguanidine structure such as 53226 (EP0825178A and WO2001/87829), derivatives having an aminoimidazole structure (WO2005/26173), derivatives having a tetrahydroisoquinolin structure (WO2004/85404) and the like have been known.
Although derivatives having a structure represented by formula (I) wherein one of R6, R7, R8, R9 and R10 is a sulfonamide group have been reported as derivatives having a monoacylguanidine structure (WO2002/24637), no specific substitution position, no functional group other than sulfonamide group or no specificity is described. In addition, although derivatives having a structure represented by formula (I) wherein X is selected from the group consisting of a single bond, an oxygen atom and a sulfur atom have been reported as NHE inhibitors (Japanese Patent Unexamined Publication Hei 10-175939), no specific substitution position or no specific function group which is required for R6, R7, R8, R9 and R10 is described.
Any excellent NHE3 inhibitor or any NHE3 inhibitor targeted for diseases or conditions of organs in which NHE3 is expressed has not yet been obtained and thus there has been a demand for such a NHE3 inhibitor.
An object of the present invention is to provide a pharmaceutical which possesses an inhibitory effect on NHE3 (Na+/H+ exchanger type 3) and effectively improves diseases or conditions of organs in which NHE3 is expressed.
Another object of the present invention is to provide a novel acylguanidine compound.
Yet another object of the present invention is to provide a novel acylguanidine compound which has good oral absorption.
Further, another object of the present invention is to provide a pharmaceutical composition.
The inventors of the present invention have intensively studied about compounds having inhibitory effects on NHE3 which are useful as pharmaceuticals improving diseases or conditions of organs in which NHE3 is expressed. As a result, compounds of formulae (I), (II) and (III) have been found to achieve the present invention.
Namely, the present invention provides a pharmaceutical comprising, as an active ingredient, an acylguanidine compound of formula (I), (II) or (III) or a pharmaceutically acceptable salt thereof, which effectively improves diseases or conditions of organs in which NHE3 is expressed.
The inventors of the present invention have intensively studied about compounds having inhibitory effects on Na+/H+ exchanger. As a result, it has been found that novel acylguanidine compounds have excellent inhibitory effects on Na+/H+ exchanger type 3 and thus the novel acylguanidine compounds are useful as pharmaceuticals which effectively improve diseases or conditions of organs in which NHE3 is expressed to achieve the present invention.
More specifically, the present invention provides an acylguanidine compound of the following formula (I) or a pharmaceutically acceptable salt thereof.
wherein
R1 is a hydrogen atom, a halogen atom, a substituted or unsubstituted C1-6-alkyl group;
R2, R3, R4 and R5 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkenyl group, a substituted or unsubstituted C1-6-alkynyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted C1-6-alkylthio group, a substituted or unsubstituted phenyloxy group and a substituted or unsubstituted phenyl group;
X is a single bond, —O— or —S—;
R6, R7, R8, R9 and R10 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a nitro group, a nitrile group, a carboxyl group, a hydroxy group, —B(OH)2, a substituted or unsubstituted amidino group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkenyl group, a substituted or unsubstituted C1-6-alkynyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted C1-6-alkylthio group, a substituted or unsubstituted aminocarbonyl group, a substituted or unsubstituted C1-6-alkyl-carbonyl group, a substituted or unsubstituted C1-6-alkoxy-carbonyl group, a substituted or unsubstituted C1-6-alkyl-S(═O)2—NH group and —OP, or
two adjacent groups from R6, R7, R8 and R9 together may form a 5-membered or 6-membered heterocyclic ring which has one or two oxygen atom(s) as a hetero atom(s) constituting the ring; and
P is selected from the group consisting of a substituted or unsubstituted C1-6-acyl group, a substituted or unsubstituted C1-6-alkoxycarbonyl group and a substituted or unsubstituted C1-6-alkylaminocarbonyl group.
In one embodiment, the followings are preferred in formula (I).
R2, R3, R4 and R5 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkenyl group, a substituted or unsubstituted C1-6-alkynyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted C1-6-alkylthio group and a substituted or unsubstituted phenyl group; and
R6, R7, R8, R9 and R10 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a nitro group, a nitrile group, a carboxyl group, a hydroxy group, —B(OH)2, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkenyl group, a substituted or unsubstituted C1-6-alkynyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted C1-6-alkylthio group, an aminocarbonyl, a substituted or unsubstituted C1-6-alkylcarbonyl group, a substituted or unsubstituted C1-6-alkoxycarbonyl group and a substituted or unsubstituted C1-6-alkyl-S(═O)2—NH group, or two adjacent groups from R6, R7, R8 and R9 together may form a 5-membered or 6-membered heterocyclic ring which has one or two oxygen atom(s) as a hetero atom(s) constituting the ring.
The present invention also provides a compound of the following formula (II) or a pharmaceutically acceptable salt thereof.
wherein
R14 is selected from the group consisting of a hydrogen atom, a halogen atom and a substituted or unsubstituted C1-6-alkyl group;
R15 and R17 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted phenyloxy group, a substituted or unsubstituted phenyl group and a substituted or unsubstituted, 5-membered or 6-membered heterocyclic ring having one or more hetero atom(s) selected from the group consisting of a nitrogen atom, an oxygen atom and a sulfur atom, the heterocyclic ring(s) being selected from the group consisting of a pyrrole ring, a furan ring, a thiophene ring, a thiazole ring, an isothiazole ring, an oxazole ring, an isoxazole ring, an imidazole ring, a pyrazole ring, a triazole ring, a tetrazole ring, a pyrimidine ring, a piperazine ring and a morpholine ring, provided that at least one of R15 and R17 is a heterocyclic ring; and
R16, R18 and R19 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted phenyloxy group and a substituted or unsubstituted phenyl group.
The present invention further provides a compound of the following formula (III) or a pharmaceutically acceptable salt thereof.
wherein
R20 is selected from the group consisting of a hydrogen atom, a halogen atom and a substituted or unsubstituted C1-6-alkyl group;
R21, R22, R23 and R24 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted morpholine group and a substituted or unsubstituted piperazine group; and
R25, R26, R27, R28 and R29 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a nitro group, a nitrile group, a carboxyl group, a hydroxy group, —B(OH)2, a substituted or unsubstituted amidino group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkenyl group, a substituted or unsubstituted C1-6-alkynyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted aminocarbonyl group, a substituted or unsubstituted C1-6-alkylcarbonyl group, a substituted or unsubstituted C1-6-alkoxycarbonyl group and a substituted or unsubstituted C1-6-alkyl-S(═O)2—NH group, or
two adjacent groups from R26, R27, R28 and R29 together may form a 5-membered or 6-membered heterocyclic ring which has one or two oxygen atom(s) as a hetero atom(s) constituting the ring.
In one aspect, the present invention provides a pharmaceutical composition comprising a compound of formula (I), (II) or (III) or a pharmaceutically acceptable salt thereof and optionally a pharmaceutically acceptable carrier.
In another aspect, the present invention provides a pharmaceutical composition for treating or preventing a disease or condition of an organ in which NHE3 is expressed, which comprises a compound of formula (I), (II) or (III) or a pharmaceutically acceptable salt thereof and optionally a pharmaceutically acceptable carrier.
In a further aspect, the present invention provides a NHE3 inhibitor comprising a compound of formula (I), (II) or (III) or a pharmaceutically acceptable salt thereof and optionally a pharmaceutically acceptable carrier.
Terms used therein are defined hereinafter.
Examples of a halogen atom include a fluorine atom, a chlorine atom, a bromine atom and an iodine atom.
“C1-6-alkyl group” means a straight, branched, cyclic or partially-cyclic aliphatic hydrocarbon group having 1 to 6 carbon(s) and includes, for example, a methyl group, an ethyl group, a propyl group, an isopropyl group, a butyl group, an isobutyl group, a sec-butyl group, a tert-butyl group, a cyclopropylmethyl group, a cyclobutyl group, a pentyl group, an isopentyl group, a 1,1-dimethyl-propyl group, a cyclopropyl group, a cyclopentyl group, a hexyl group, a cyclohexyl group and the carbon number is preferably 1 to 3.
“C1-6-alkenyl group” means a straight, branched or cyclic alkenyl group having 1 to 6 carbon(s) and specifically includes, for example, a 1-propenyl group, a 2-propenyl group, an isopropenyl group, a 1-butenyl group, a 2-butenyl group and a 3-butenyl group.
“C1-6-alkynyl group” means a straight or branched alkynyl group having 1 to 6 carbon(s) and specifically includes, for example, an ethynyl group, a 1-propynyl group, a 2-propynyl group, a 1-butynyl group, a 2-butynyl group and a 3-butynyl group.
“C1-6-alkoxy group” means a straight, branched or cyclic alkoxy group having 1 to 6 carbon(s) and specifically includes, for example, a methoxy group, an ethoxy group, a n-propoxy group, a n-butoxy group, a n-pentyloxy group, a n-hexyloxy group, an isopropoxy group, an isobutoxy group, a sec-butoxy group, a tert-butoxy group, a cyclopropyloxy group, a cyclobutoxy group, a cyclopentyloxy group and a cyclohexyloxy group, and the carbon number is preferably 1 to 3.
“C1-6-alkylthio group” means a straight, branched or cyclic alkylthio group and specifically includes, for example, a methylthio group, an ethylthio group, a n-propylthio group, a n-butylthio group, a n-pentylthio group, a n-hexylthio group, an isopropylthio group, an isobutylthio group, a sec-butylthio group and a tert-butylthio group, and the carbon number is preferably 1 to 3.
“A 5-membered or 6-membered heterocyclic ring which has one or two oxygen atom(s) as a hetero atom(s) constituting the ring” is preferably, but not specifically limited to, a 5-membered or 6-membered heterocyclic ring which has two oxygen atoms as hetero atoms constituting the ring and the rings represented by the following formulae are most preferable.
“Substituted” means that a group modified with the term has at least one substituent(s) selected from the following atoms or groups. Each substituent may be identical or different, and substitution position or substitution number may be any position or number and are not specifically limited.
Substituents are selected from the group consisting of halogen atoms, a hydroxy group, a mercapto group, a nitro group, a cyano group, an alkyl group, an alkoxy group, an alkylthio group, an alkylsulfonyl group, an acyl group, an acyloxy group, a carboxyl group, an alkoxycarbonyl group, a carbamoyl group, a sulfonamide group, an aryl group and a hetero aryl group.
“Substituted or unsubstituted” means that a group modified with the term may have no substituent or may have one or more substituent(s). Such substituents may be identical or different, and substitution position or substitution number may be any position or number and is not specifically limited. Such substituents preferably are selected from the group consisting of halogen atoms, a hydroxy group, a mercapto group, a nitro group, a cyano group, an alkyl group, an alkoxy group, an alkylthio group, an alkylsulfonyl group, an acyl group, an acyloxy group, a carboxyl group, an alkoxycarbonyl group, a carbamoyl group, a sulfonamide group, an aryl group and a heteroaryl group.
The compounds of the present invention are compounds of formulae (I), (II) and (III) and have an acryloyl group. Based on such structures, there are cis-trans geometrical isomers (or (E) isomer and (Z) isomer). The present invention encompasses an individual isomer ((E) isomer or (Z) isomer) or a mixture of the isomers. Among the compounds of the present invention, compounds having a trans configuration are particularly preferred. In addition, in the compounds of the present invention, there are tautomers based on their acylguanidine structure. The present invention encompasses an individual tautomer or a mixture of the tautomers. Other than the above-explained isomers or tautomers, there may be geometrical isomers or tautomers depending on kinds of substituents. The present invention encompasses an individual isomer or tautomer or a mixture of such isomers or tautomers. The compounds of the present invention may have an asymmetric carbon atom and in that case, there may be enantiomers (optical isomers) of (R) isomer and (S) isomer based on the asymmetric carbon atom. The present invention encompasses an individual enantiomer or a mixture of the enantiomers.
In the present invention, compounds having a combination of preferable groups for each substituent are preferable.
More specifically, the following groups for each substituent in formula (I) are preferable as an acylguanidine compound of formula (I) or a pharmaceutically acceptable salt thereof.
X is preferably a single bond or —O—, and more preferably a single bond.
R1 is preferably selected from the group consisting of a hydrogen atom, a halogen atom, a methyl group and an ethyl group, and more preferably selected from a hydrogen atom or a methyl group.
R2, R3, R4 and R5 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a methyl group, an ethyl group, a methoxy group, an ethoxy group and a phenyl group substituted with a hydroxy group, more preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a methyl group and a methoxy group, and most preferably are selected from the group consisting of a hydrogen atom, a halogen atom and methyl group.
R6, R7, R8, R9 and R10 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a nitro group, a nitrile group, a carboxyl group, a hydroxy group, —B(OH)2, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy-carbonyl group, a substituted or unsubstituted C1-6-alkyl-S(═O)2—NH group, a substituted or unsubstituted amidino group and a substituted or unsubstituted aminocarbonyl group, more preferably selected from the group consisting of a carboxyl group, a hydroxy group, —B(OH)2, a 1-hydroxyethyl group, CH3—S(═O)2—NH group, an amidino group and HONHC(═O) group, and most preferably are a hydroxy group.
In addition to those preferable groups, R7, R8 and R9 are each independently preferably a hydroxy group, and R8 is most preferably a hydroxy group.
Compounds having a combination of the following groups for each substituent in formula (I) are preferable as an acylguanidine compound of formula (I) or a pharmaceutically acceptable salt thereof.
R5 is a hydrogen atom or a methyl group and R6 and R10 are each independently selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a methoxy group and a substituted or unsubstituted C1-6-alkyl group.
Further, R2 is more preferably a hydrogen atom.
More preferably, R1 is a hydrogen atom or a C1-6-alkyl group.
Moreover, compounds having a combination of the following groups for each substituent in formula (I) are also preferable as an acylguanidine compound of formula (I) or a pharmaceutically acceptable salt thereof.
R3 is selected from the group consisting of a hydrogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6 alkoxy group and a substituted or unsubstituted phenyl group and R4 is selected from the group consisting of a hydrogen atom, a fluorine atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted phenyloxy group and a substituted or unsubstituted phenyl group.
In addition, in the definitions for each substitutent, the substituted or unsubstituted phenyl group is preferably selected from the group consisting of a unsubstituted phenyl group and a hydroxy phenyl group, and the substituted or unsubstituted phenyloxy group is preferably selected from the group consisting of a unsubstituted phenyloxy group and a hydroxyphenyloxy group.
Furthermore, in the definitions for each substituent, each “C1-6” is more preferably C1-3.
The following groups for each substituent in formula (II) are preferable as an acylguanidine compound of formula (II) or a pharmaceutically acceptable salt thereof.
R14 is preferably selected from the group consisting of a hydrogen atom and a substituted or unsubstituted C1-6-alkyl group.
R16 is preferably a hydrogen atom or a methyl group.
R15 and R17 are each independently preferably selected from a substituted or unsubstituted, 5-membered or 6-membered hetero ring which contains therein one or more hetero atom(s) selected from the group consisting of nitrogen, oxygen and sulfur, more preferably selected from the group consisting of a substituted or unsubstituted furan ring, a substituted or unsubstituted pyrrole ring, a substituted or unsubstituted thiophene ring, a substituted or unsubstituted pyrazole ring and a substituted or unsubstituted imidazole ring, and most preferably a pyrrole ring.
R19 is preferably selected from the group consisting of a hydrogen atom, a halogen atom and a methyl group.
If R15 is selected from the above-described hetero ring, R17 is preferably selected from the group consisting of a hydrogen atom, a fluorine atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group and a substituted or unsubstituted C1-6-alkoxy group.
Furthermore, in the definitions for each substituent, each “C1-6” is more preferably C1-3.
In addition, compounds having a combination of the following groups for each substituent in formula (II) are preferable as an acylguanidine compound of formula (II) or a pharmaceutically acceptable salt thereof.
R14 is preferably selected from the group consisting of a hydrogen and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom and a methyl group.
R16 is preferably a hydrogen atom or a methyl group.
R17 is preferably selected from the group consisting of a hydrogen atom, a fluorine atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group and a substituted or unsubstituted C1-6-alkoxy group.
R15 is preferably selected from a substituted or unsubstituted, 5-membered or 6-membered hetero ring which contains therein one or more hetero atom(s) selected from the group consisting of nitrogen, oxygen and sulfur, more preferably selected from the group consisting of a substituted or unsubstituted furan ring, a substituted or unsubstituted pyrrole ring, a substituted or unsubstituted thiophene ring, a substituted or unsubstituted pyrazole ring and a substituted or unsubstituted imidazole ring, and most preferably a pyrrole ring.
Furthermore, in the definitions for each substituent, each “C1-6” is more preferably C1-3.
Moreover, compounds having a combination of the following groups for each substituent in formula (II) are also preferable as an acylguanidine compound of formula (II) or a pharmaceutically acceptable salt thereof.
R14 is preferably selected from the group consisting of a hydrogen atom and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom and a methyl group.
R15 and R19 are each independently preferably selected from the group consisting of a hydrogen atom and a methyl group.
R17 is preferably selected from a substituted or unsubstituted, 5-membered or 6-membered hetero ring which contains therein one or more hetero atom(s) selected from the group consisting of nitrogen, oxygen and sulfur, and more preferably selected from the group consisting of a substituted or unsubstituted furan ring, a substituted or unsubstituted pyrrole ring, a substituted or unsubstituted thiophene ring, a substituted or unsubstituted pyrazole ring and a substituted or unsubstituted imidazole ring.
Furthermore, in the definitions for each substituent, each “C1-6” is more preferably C1-3.
The following groups for each substituent in formula (III) are preferable as an acylguanidine compound of formula (III) or a pharmaceutically acceptable salt thereof.
R21 and R24 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted morpholine group and a substituted or unsubstituted piperazine group.
R22 and R23 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom, a halogen atom, a methyl group and an ethyl group.
R25 and R29 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom, a halogen atom, a methyl group and an ethyl group.
One of R25, R26, R27, R28 and R29 is preferably a hydroxy group, and more preferably, one of R26, R27 and R28 is a hydroxy group.
Moreover, compounds having a combination of the following groups for each substituent in formula (III) are also preferable as an acylguanidine compound of formula (III) or a pharmaceutically acceptable salt thereof.
R21 and R24 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a substituted or unsubstituted C1-6-alkyl group, a substituted or unsubstituted C1-6-alkoxy group, a substituted or unsubstituted morpholine group and a substituted or unsubstituted piperazine group, more preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a methyl group, an ethyl group, a methoxy group, an ethoxy group and morpholine group, and most preferably selected from the group consisting of a hydrogen atom, a methyl group and a morpholine group.
R22 and R23 are each independently preferably selected from the group consisting of a hydrogen atom and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom and a methyl group.
R25 and R29 are each independently preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group and a substituted or unsubstituted C1-6-alkyl group, and more preferably selected from the group consisting of a hydrogen atom, a halogen atom, a hydroxy group, a methoxy group, a methyl group and an ethyl group.
One of R26, R27 and R28 is preferably selected from the group consisting of a hydroxymethyl group and a hydroxy group, and more preferably, R27 is a hydroxy group.
Furthermore, in the definitions for each substituent, each “C1-6” is more preferably C1-3-
Representative methods for manufacturing the present compounds of formulae (I), (II) and (III) will be explained hereinafter. Most of the compounds of the present invention can be synthesized, for example, by using the following manufacturing methods.
In the above described Manufacturing method A, R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are those defined hereinbefore. R11, R12 and R13 are each independently selected from the group consisting of a hydrogen atom, a C1-5-alkyl group which may be substituted with a halogen atom and benzyl group which may be substituted with a halogen atom. In addition, two R11 substitutents may form a ring by sharing a substituent or binding together.
Corresponding aldehyde (2A) can be synthesized by coupling corresponding bromoaldehyde (1A) with a corresponding phenylboronicacid derivative. Corresponding acrylic acid ester (3A) can be synthesized by reacting the resultant aldehyde (2A) with a corresponding phosphoryl derivative which has been treated under low temperature and under basic condition such as NaH, lithium diisopropylamide (LDA) and n-BuLi. Corresponding acrylic acid (4A) can be synthesized by hydrolyzing the resultant acrylic acid ester (3A) such as under an alkaline condition. Acylguanidine (IV) of the present invention can be synthesized by activating the resultant acrylic acid (4A) via an addition of a condensation agent such as 1,1′-carbonylbis-1H-imidazole (CDI) thereto and then adding 1M solution of guanidine-dimethylformaldehyde (DMF).
In the above described Manufacturing method B, R1, R2, R3, R4, R5, R6, R7, R8, R9 and R10 are those defined hereinbefore. R11, R12 and R13 are each independently selected from the group consisting of a hydrogen atom, a C1-5-alkyl group which may be substituted with a halogen atom and benzyl group which may be substituted with a halogen atom. In addition, two R11 substitutents may form a ring by sharing a substituent or binding together.
Corresponding acrylic acid ester (2B) can be synthesized by reacting corresponding 2-bromoaldehyde (1B) with a corresponding phosphoryl derivative which has been treated under low temperature and under basic condition such as NaH, lithium diisopropylamide (LDA) and n-BuLi. Corresponding acrylic acid (3B) can be synthesized by hydrolyzing the resultant acrylic acid ester (2B) such as under an alkaline condition. Acylguanidine (4B) can be synthesized by activating the resultant acrylic acid (3B) via an addition of a condensation agent such as 1,1′-carbonylbis-1H-imidazole (CDI) thereto and then adding 1M solution of guanidine-dimethylformaldehyde (DMF). Acylguanidine (IV) of the present invention can be synthesized by coupling the resultant acylguanidine (4B) with a corresponding phenylboronicacid derivative.
In the above described Manufacturing method C(C-1, C-2 and C-3), R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R14, R15, R16, R17, R18, R19, R20, R21, R22, R23, R24, R25, R26, R27, R28 and R29 are those defined hereinbefore. R11, R12 and R13 are each independently selected from the group consisting of a hydrogen atom, a C1-5-alkyl group which may be substituted with a halogen atom and benzyl group which may be substituted with a halogen atom. In addition, two R11 substitutents may form a ring by sharing a substituent or binding together.
Corresponding acrylic acid ester (2C) can be synthesized by reacting corresponding 2-bromoaldehyde (1C) with a corresponding phosphoryl derivative which has been treated under low temperature and under basic condition such as NaH, lithium diisopropylamide
(LDA) and n-BuLi. Corresponding acrylic acid (3C) can be synthesized by hydrolyzing the resultant acrylic acid ester (2C) such as under an alkaline condition. Acylguanidine (4C) can be synthesized by activating the resultant acrylic acid (3C) via an addition of a condensation agent such as 1,1′-carbonylbis-1H-imidazole (CDI) thereto and then conducting a condensation reaction with a guanidine protected with a tert-butoxycarbonyl (Boc) group. Acylguanidines (IV), (V) and (VI) of the present invention can be synthesized by coupling the resultant acylguanidine (4C) with a corresponding phenylboronicacid derivative.
The condensation of an acrylic acid with a guanidine derivative in the above described manufacturing methods A, B and C can be carried out by using any conventional method in the art and examples of such a conventional method include use of an acid halide, an acid anhydride, an active ester, a lower alkylester, an acid azide, an condensation agent.
Examples of such an acid halide include acid chlorides and acid bromides.
A symmetric acid anhydride or a mixed acid anhydride may be used as an acid anhydride and examples of such a mixed acid anhydride include a mixed acid anhydride with an alkyl chlorocarbonate ester such as ethyl chlorocarbonate and isobutyl chlorocarbonate, a mixed acid anhydride with an aralkyl chlorocarbonate ester such as benzyl chlorocarbonate, a mixed acid anhydride with an aryl chlorocarbonate ester such as phenyl chlorocarbonate and a mixed acid anhydride with an alkane acid such as isovaleric acid and pivalic acid.
Examples of such an active ester include p-nitrophenyl ester, N-hydroxysuccinimide ester, pentafluorophenyl ester, 2,4,5-trichlorophenyl ester, pentachlorophenyl ester, cyanomethyl ester, N-hydroxysuccinimide ester, N-hydroxyphthalimide ester, N-hydroxy-5-norbornene-2,3-dicarboxyimide ester, N-hydroxypiperidine ester, 8-hydroxyquinoline ester, 2-hydroxyphenyl ester, 2-hydroxy-4,5-dichlorophenyl ester, 2-hydroxypiperidine ester, 2-pyridylthiol ester and 1-benzotriazole.
Examples of such a condensation agent include, for example, dicyclohexylcarbodiimide (DCC), diisopropylcarbodiimide (DIPC), 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (WSC), benzotriazole-1-yl-tris(dimethylamino) phosphonium-hexafluorophosphate (BOP), diphenylphosphonylazide (DPPA), 1,1′-carbonyl bis-1H-imidazole (CDI) and the like.
If desired, an additive such as N-hydroxysuccinimide (HONSu), 1-hydroxybenzotriazole (HOBt, 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine (HOOBt) may be further added.
In each step, any reaction conditions generally used in the art can be used and should be appropriately selected depending on kinds of staring compounds.
In addition, examples of a solvent used include, for example, an aromatic hydrocarbon solvent such as benzene, toluene and xylene, an ether solvent such as tetrahydrofuran and 1,4-dioxane, a halogenated hydrocarbon solvent such as dichloromethane, chloroform and 1,2-dichloroethane, an amide solvent such as dimethylformamide and dimethylacetamide, and a basic solvent such as pyridine. Each solvent may be used by itself or in combination with one or more other solvent(s) including water. Solvent(s) should be appropriately selected depending on kinds of starting compounds.
Manufacturing method C may be preferably used to carry out the present invention and CDI may be preferably used as a condensation agent.
The compounds of the present invention obtained by the above-explained methods can be purified by any conventional means generally used in the organic synthesis field such as extraction, distillation, crystallization, column chromatography and the like.
In the case where compounds of formulae (I), (II) and (III) according to the present invention may form a salt, such a salt may be any kind of salt as long as it is pharmaceutically acceptable. If there is an acidic group in a compound such as carboxyl group, examples of such a salt include, for example, an ammonium salt, a salt with an alkali metal such as sodium and potassium, a salt with an alkaline earth metal such as calcium and magnesium, an aluminum salt, a zinc salt, a salt with an organic amine such as triethylamine, ethanolamine, morpholine, piperazine and dicyclohexylamine, and a salt with a basic amine such as arginine and lysine for such an acidic group.
If there is a basic group in a compound, examples of such a salt include, for example, a salt with an inorganic acid such as hydrochloric acid, sulfuric acid, phosphoric acid, nitric acid, and hydrobromic acid, a salt with an organic carboxylic acid such as acetic acid, trifluoroacetic acid, citric acid, benzoic acid, maleic acid, fumaric acid, tartaric acid, succinic acid, tannic acid, butyric acid, hibenzoic acid, pamoic acid, enanthic acid, decanoic acid, teoclate, salicyclic acid, lactic acid, oxalic acid, mandelic acid and malic acid, and a salt with an organic sulfonic acid such as methanesulfonic acid, benzenesulfonic acid and p-toluenesulfonic acid.
Examples of a method of forming a salt include, for example, mixing a compound of formula (I), (II) or (III) with an appropriate acid or base at a suitable ratio in a solvent or dispersion, or conducting cation exchange or anion exchange from another salt form.
The compounds of the present invention encompass solvates of a compound of formula (I), (II) or for example, hydrates, alcohol adducts and the like.
The compounds of the present invention may be converted to corresponding prodrug forms. The term “prodrug” used herein means a compound which will be converted (metabolized) in the body into the compound of the present invention. For instance, in the case where an active form has a carboxyl group or phosphate group, examples of a prodrug include their esters, amides and the like. In the case where an active form has an amino group, examples of a prodrug include its amides, carbamate and the like. In the case where an active form has a hydroxy group, examples of a prodrug include its esters, carbonates, carbamates and the like. The compounds of the present invention may be converted into corresponding prodrugs by combining them with amino acid(s) or sugar group(s).
The acylguanidine derivatives of the present invention of formulae (I), (II) and (III) or a pharmaceutically acceptable salt thereof may be produced as a pharmaceutical composition with or without using a drug formulation auxiliary agent according to conventional means and then administered. Examples of a dosage form for the pharmaceutical composition include, for example, tablet, powder, injection solution, freeze-dried form for injection, pill, granule, capsule, suppository, liquid, sugar coated tablet, depot, syrup, suspension, emulsion, troche, sublingual tablet, patch, orally-disintegrating tablet, inhalant, enema, ointment, tape, eye drop and the like.
The pharmaceutical composition or NHE3 inhibitor of the present invention may comprise any one of or any combination of two, three or more of the acylguanidine compounds of formulae (I), (II) and (III) or a pharmaceutically acceptable salt thereof and may further comprise any pharmaceutically, physiologically or experimentally acceptable, solid or liquid carriers, additives and the like.
Examples of such a carrier includes, for example, glucose, lactose, sucrose, starch, mannitol, dextrin, fatty acid glycerides, polyethylene glycol, hydroxyethylated starch, ethylene glycol, polyoxyethylene sorbitan fatty acid esters, gelatin, albumin, amino acids, water and saline. Moreover, any conventional additives such as stabilizing agents, wetting agents (humectants), emulsifying agents, binders, tonicity agents and the like may be appropriately added to the pharmaceutical composition or NHE3 inhibitor of the present invention, if necessary.
Examples of such an additive include, but not specifically limited to as long as they are generally used in the art for any purpose, for example, flavors, saccharides, sweeteners, dietary fibers, vitamins, amino acids such as monosodium glutaminate (MSG), nucleic acids such as inosine monophosphate (IMP), mineral salts such as sodium chloride, water and the like.
In addition, the pharmaceutical composition or NHE3 inhibitor may be used in any form such as dry powder, paste, solution and the like.
The pharmaceutical composition or NHE3 inhibitor of the present invention may be applied via any invasive or noninvasive administration method. Examples of such a method include, but not specifically limited to, oral administration, injection and the like. Administration of suppository or transdermal administration may be also employed.
An active ingredient may be formulated in any conventional pharmaceutical formulation together with any solid or liquid pharmaceutical carrier suitable for oral administration or injection and then administered. Examples of such a formulation include, for example, a solid formulation such as tablet, granule, powder and capsule, a liquid formulation such as solution, suspension and emulsion, and freeze dried formulation. These formulations can be prepared by any conventional means in the art. In addition, any pharmaceutically or experimentally acceptable, solid or liquid carriers, additives and the like may be optionally added to the pharmaceutical composition or NHE3 inhibitor of the present invention.
Although an amount of the pharmaceutical composition or NHE3 inhibitor of the present invention may be appropriately determined depending on each purpose, for instance, if it is orally administered to the subject, as the total amount of the acylguanidine compounds of formulae (I), (II) and (III) or a pharmaceutically acceptable salt thereof, it is preferably 0.0001 mg/kg˜5 g/kg of body weight per dose, more preferably 0.001 mg/kg˜1 g/kg of body weight per dose, and yet more preferably 0.01 mg/kg˜10 mg/kg of body weight per dose. Number of administration times is not specifically limited and it may be administered 1 time or plural times/day.
Although a content of the acylguanidine compounds of formulae (I), (II) and (III) or a pharmaceutically acceptable salt thereof in the pharmaceutical composition or NHE3 inhibitor is not specifically limited as long as it complies with the above-described amount to be used, it is preferably 0.000001 mass %˜99.9999 mass % based on the dry weight of the pharmaceutical composition or NHE3 inhibitor, more preferably 0.00001 mass %˜99.999 mass %, and particularly preferably 0.0001 mass %˜99.99 mass %.
The pharmaceutical composition or NHE3 inhibitor of the present invention may further comprise one or two or more kind(s) of known substance(s) which can produce clinically desired effect(s).
The pharmaceutical composition or NHE3 inhibitor can be used for any disease or condition for which it may produce clinically desired therapeutic or preventive effect(s) including NHE3-related diseases or conditions. Examples of such a disease or condition include, but not limited to, renal dysfunction, diabetic nephropathy, metabolic syndrome-related nephropathy, edema, hypertension, sleep apnea syndrome, renal ischemia, reperfusion injury and tubular damage, and tubular damage or renal dysfunction is preferred.
The acylguanidine compounds of formulae (I), (II) and (III) or a pharmaceutically acceptable salt thereof according to the present invention show good inhibitory effects on Na+/H+ exchanger type 3. Particularly preferred compounds among the compounds according to the present invention have good oral absorption. In addition, particularly preferred compounds among the compounds according to the present invention have good selectivities for NHE3.
The present invention will be explained in detail hereinafter by referring to the examples, which are not intended to be limiting of the present invention.
AIBN: azoisobutyronitrile
Boc: tert-butoxycarbonyl
CDI: 1,1′-carbonylbis-1H-imidazole
DMA: dimethylacetamide
DMF: dimethylformamide
dppf: 1,1′-bis(diphenylphosphino) ferrocene
EtOAc: ethylacetate
EDCI: 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride
HATU: O-(benzotriazol-1-yl)-N,N,N′,N′-tetramethyluroniumhexafluorophosphate
HPLC: high performance liquid chromatograph
MeOH: methanol
MS: analytical value by mass spectrometry (EI) [M+H]+
TFA: trifluoroacetic acid
THF: tetrahydrofuran
NaH (60% assay, 824 mg, 20.6 mmol) was suspended in DMF (50 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (4.48 mL, 20.6 mmol) in DMF (10 mL) was added dropwise in a slow manner to the resulting solution and stirred for 15 minutes. Then, 2-bromobenzaldehyde (2.0 mL, 17.0 mmol) in DMF (3 mL) was added thereto in a slow manner and stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue.
The resulting residue was dissolved in THF (50 mL) and MeOH (20 mL), 1 N NaOH (40 ml, 40 mmol) was added thereto and stirred at room temperature for 8 hours. The solvent was eliminated in vacuo, 2N HCl was added to acidify the solution and then the precipitated crystals were filtrated to obtain white crystals of the objective carboxylic acid (3.37 g, 82.0%).
MS: 241
The carboxylic acid obtained from Step 1 (3.81 g, 15.7 mmol) was dissolved in DMF (40 mL), CDI (2.80 g, 17.3 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (3.75 g, 23.6 mmol) was added to the solution and then stirred for 16 hours. After eliminating the solvent in vacuo, TFA (10 mL) was added to the residue at 0° C. and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective Intermediate 1 (3.48 g, 57.0%).
MS: 282
NaH (60% assay, 502 mg, 12.6 mmol) was suspended in DMF (50 mL), triethyl-phosphonopropionate (2.74 mL, 12.6 mmol) was added dropwise in a slow manner to the resulting solution and then stirred for 15 minutes. Then, 2-bromo-4-methylbenzaldehyde (2 g, 10.1 mmol) in DMF (10 mL) was added thereto in a slow manner and stirred for 18 hours. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. After eliminating the solvent in vacuo, the resulting compound was dissolved in THF (15 mL) and MeOH (12 mL), 1N NaOH (8 mL, 8 mmol) was added and then stirred at room temperature for 8 hours. The solvent was eliminated in vacuo, 2N HCl was added to acidify the solution and then the precipitated crystals were filtrated to obtain the objective carboxylic acid (1.36 g, 42%).
MS: 241
The carboxylic acid obtained from Step 1 (1.36 g, 5.3 mmol) was dissolved in DMF (10 mL), CDI (1.0 g, 6.4 mmol) was added and then stirred at room temperature for 30 minutes. N-Boc-guanidine (1.27 g, 8.0 mmol) was added to the solution and stirred for 16 hours. EtOAc was added, washed with water and saturated saline and then dried over anhydrous MgSO4. TFA (10 mL) was added to the residue and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective Intermediate 2 (820 mg, 38%).
MS: 297
Intermediate 3 was obtained in the same manner as described for Intermediate 1.
MS: 362
AIBN (657 mg, 4.0 mmol) was added at room temperature into a container containing 2,4-dibromotoluene (5.00 g, 20 mmol), N-bromosuccinimide (3.92 g, 22.0 mmol) and carbon tetrachloride (6.0 mL). After stirring it at 65° C. for 16 hours, the solvent was eliminated in vacuo. The residue was filtrated, washed with hexane and then the filtrate was eliminated in vacuo to obtain a crude product (5.12 g, 78 W.
Tri-methylamine-N-oxide (1.17 g, 15.6 mmol) was added to the crude product obtained from Step 1 (5.12 g, 15.6 mmol) in acetonitrile (30 mL) and then stirred at 60° C. for 6 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective aldehyde (2.49 g, 60%).
MS: 265
NaH (60% assay, 1.13 g, 28.3 mmol) was suspended in THF (40 mL) and then cooled to 0° C. Tri-ethyl-2-phosphonopropionate (6.74 g, 28.3 mmol) in THF (5 mL) was added in a slow manner to the resulting suspension. After stirring it for 15 minutes, the aldehyde obtained from Step 2 (2.49 g, 9.435 mmol) in THF (5 mL) was added thereto and then stirred for 1 hour while gradually raising the temperature to room temperature. EtOAc was added thereto and then washed with NaHCO3 solution, water and saturated saline. After drying it over anhydrous MgSO4, the solvent was eliminated in vacuo to obtain a crude product (an ester intermediate).
MS: 349
The resulting crude product was dissolved in a mixed solution of THF/MeOH (v/v=5/3, 40 mL). Then, 2N NaOH (30 mL, 60 mmol) was added to the solution and stirred at 50° C. for 6 hours. After cooling it to 0° C., 2N HCl was added to acidify the solution, dichloromethane was added thereto, washed with water and saturated saline and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective carboxylic acid (2.04 g, 68%).
MS: 321
The carboxylic acid obtained from Step 3 (2.04 g, 6.375 mmol) was dissolved in DMF (20 mL), CDI (1.24 g, 7.65 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (1.22 g, 7.65 mmol) was added to the solution and then stirred for 19 hours. Then, the solvent was eliminated in vacuo, TFA (20 mL) was added to the residue and stirred at 55° C. for 8 hours. Then, the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective Intermediate 4 (0.821 g, 27%).
MS: 362
NaH (60% assay, 412 mg, 10.3 mmol) was suspended in DMF (50 mL) and then cooled to 0° C. Tri-ethyl-2-phosphonopropionate (2.24 mL, 10.3 mmol) in THF (10 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, 4-bromobenzaldehyde (1.57 g, 8.49 mmol) in DMF (3 mL) was added thereto in a slow manner and stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue.
The resulting residue was dissolved in THF (50 mL) and MeOH (20 mL), 1N NaOH (40 mL, 40 mmol) was added and then stirred at room temperature for 8 hours. The solvent was eliminated in vacuo, 2N HCl was added to acidify the solution and the precipitated crystals was filtrated to obtain white crystals of the objective carboxylic acid (729 mg, 35%).
MS: 242
The carboxylic acid obtained from Step 1 (729 mg, 3.0 mmol) was dissolved in DMF (20 mL), CDI (535 g, 3.3 mmol) was added and then stirred at room temperature for 30 minutes.
N-Boc-guanidine (720 mg, 4.5 mmol was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (10 mL) was added to the residue at 0° C. and then stirred for 1.5 hours. After vacuum concentration of the solvent, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective Intermediate 5 (348 mg, 29%).
The Intermediate 6 (330 mg, 17%) was obtained from 4-bromo-2-methylbenzaldehyde (1.0 g) in the same manner as described for Intermediate 1.
MS: 297
Intermediate 1 (20 mg, 0.05 mmol) and 4-chlorophenylboronic acid (9 mg. 0.055 mmol were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (3 mg, 2.6 μmol and Na2CO3 (21 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 1 (5.7 mg, 27% MS: 314
Intermediate 1 (50 mg. 0.126 mmol) and 4-hydroxyphenylboronic acid (19.2 mg, 0.139 mmol were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.4 mL). Pd(PPh3)4 (7.29 mg, 6.30 μmol) and Na2CO3 (40.1 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 15.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 2 (51.6 mg, 100%). 1H-NMR (d-DMSO, 300 MHz), σ 2.01 (s, 3H), 6.82 (d, 2H, J=8.5 Hz), 7.13 (d, 2H, J=8.5 Hz), 7.33 (s, 1H), 7.37-7.52 (m, 4H), 8.19-8.33 (bs, 4H), 9.66 (s, 1H)
MS: 296
Intermediate 1 (20 mg, 0.05 mmol) and 4-methoxyphenylboronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg. 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 3 (6.6 mg, 31%).
MS: 310
<Step 1>2-bromobenzaldehyde (200 mg, 1.08 mmol) and 4-ethoxyphenylboronic acid (179 mg, 1.08 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 8 mL). Pd(PPh3)4 (125 mg, 0.108 mmol) and Na2CO3 (343 mg, 3.24 mmol) were added to the solution and then stirred at 90° C. for 6 hours. After cooling it to room temperature, EtOAc was added thereto, washed with NaHCO3 solution, water and saturate saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo and then purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective aldehyde (202 mg, 82.6%).
1H-NMR (d-DMSO, 300 MHz) σ 1.46 (t, 3H, J=7.0 Hz), 4.10 (q, 2H, J=7.0 Hz), 6.99 (d, 2H, J=8.5 Hz), 7.26 (s, 1H), 7.30 (d, 2H, J=8.5 Hz). 7.39-7.50 (m, 2H), 7.62 (ddd, 2H, J=1.5, 7.3, 7.3 Hz), 7.39-7.50 (m, 2H, J=1.1, 7.3 Hz), 10.0 (s, 1H)
MS: 227
NaH (60% assay, 53.6 mg, 1.34 mmol) was suspended in THF (5 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (319 mg, 1.34 mmol) in THF (2 mL) was added in a slow manner to the suspension. After stirring it for 15 minutes, the aldehyde obtained from Step 1 (202 mg, 0.893 mmol) in THF (2 mL) was added thereto and then stirred overnight while gradually heating it to room temperature. EtOAc was added thereto, washed with NaHCO3, water and saturated saline, and then dried over anhydrous MgSO4. After eliminating the solvent in vacuo, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective ester (258 mg, 93.0%).
1H-NMR (d-DMSO, 300 MHz) σ 1.26 (t, 3H, J=7.0 Hz), 1.44 (t, 3H, J=7.0 Hz), 2.01 (s, 3H), 4.07 (q, 2H, J=7.0 Hz), 4.20 (q, 2H, J=7.0 Hz), 6.91 (d, 2H, J=8.8 Hz), 7.23 (d, 2H, J=8.8 Hz), 7.31 (s, 1H), 7.33-7.40 (m, 3H), 7.54 (bs, 1H), 7.98 (d, 1H, J=16 Hz)
MS: 311
The ester obtained from Step 2 (258 mg, 0.831 mmol) was dissolved in a mixed solution of THF and MeOH (v/v=4/1, 5.2 mL). 2N NaOH (4.2 mL, 8.31 mmol) was added to the solution and then stirred at room temperature for 63.5 hours. After the solvent was eliminated in vacuo, 2N HCl (4.2 mL) was added thereto to acidify the solution, EtOAc was added thereto, washed with water and saturated saline, and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a quantitative amount of the objective carboxylic acid (235 mg).
MS: 283
The carboxylic acid obtained from Step 3 (50 mg, 0.177 mmol was dissolved in DMF (3 mL), CDI (31.6 mg, 0.195 mmol) was added thereto and then stirred at room temperature for 30 minutes. 2N guanidine solution in DMF (0.266 mL, 0.531 mmol) was added to the solution and then stirred at room temperature for 21 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 4 (2.2 mg, 2.84%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 4-acetylphenylboronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 5 (6.8 mg, 31.0%).
MS: 322
Intermediate 1 (20 mg, 0.05 mmol) and 4-hydroxymethylphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 6 (7.8 mg, 38%).
MS: 310
Intermediate 1 (20 mg, 0.05 mmol) and 4-methoxycarbonylphenyl boronic acid (11 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 7 (3.3 mg, 15%).
MS: 338
Intermediate 1 (20 mg, 0.05 mmol) and 4-methanesulfonamidephenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 8 (9.9 mg, 41%).
MS: 373
Intermediate 1 (20 mg, 0.05 mmol) and 4-carboxyamidephenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1 TFA in water/CH3CN) to obtain the compound of Example 9 (3.1 mg, 14%).
MS: 323
Intermediate 1 (20 mg, 0.05 mmol) and 4-carboxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 10 (4.2 mg, 19%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 1,4-benzenediboronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.4 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.152 mmol) were added to the solution and then stirred at 90° C. for 15.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 11 (5.0 mg, 23%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 4-nitrophenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 12 (3.8 mg, 17%).
MS: 325
Intermediate 1 (20 mg, 0.05 mmol) and 3-hydroxyphenylboronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 mol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 13 (5.6 mg, 27%).
MS: 296
The compound of Example 15 (10 mg, 0.0229 mmol) was dissolved in THF (1 mL), cooled to 0° C., NaBH4 (2 mg, 0.046 mmol) was added thereto and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 14 (4.0 mg, 40%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 4-acetylphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 15 (4.3 mg, 20%).
MS: 322
Intermediate 1 (20 mg, 0.05 mmol) and 3-methoxycarbonylphenylboronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 16 (3.3 mg, 31%).
MS: 338
Intermediate 1 (20 mg, 0.05 mmol) and 3-methanesulfonamidephenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred overnight at 90° C. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 17 (6.3 mg, 27%).
MS: 373
Intermediate 1 (20 mg, 0.05 mmol) and 3-carboxyamidephenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 18 (4.6 mg, 21%).
MS: 323
Intermediate 1 (20 mg, 0.05 mmol) and 3-carboxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 19 (6.5 mg, 30%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 3-cyanophenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 20 (4.1 mg, 20%).
MS: 305
Intermediate 1 (20 mg, 0.05 mmol) and 2-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 21 (5.6 mg, 27%).
MS: 296
Intermediate 1 (20 mg, 0.05 mmol) and 3,5-dimethyl-4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.4 mL). Pd(PPh3)4 (7.29 mg, 6.30 μmol) and Na2CO3 (40.1 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 15.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 22 (5.0 mg, 23%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 3-methoxy-4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 23 (5.2 mg, 24%).
MS: 326
Intermediate 1 (20 mg, 0.05 mmol) and 3-fluoro-4-hydroxyphenyl boronic acid (10.2 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.4 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.152 mmol) were added to the solution and then stirred at 90° C. for 15.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 24 (3.0 mg, 14%).
MS: 314
The intermediate obtained from Step 2 of Example 32 (20 mg, 0.05 mmol) and 3,5-difluoro-4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 25 (5.2 mg, 24%).
MS: 318
Intermediate 1 (20 mg, 0.05 mmol) and 3,4-dihydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 26 (5.3 mg, 25 W.
MS: 312
Intermediate 1 (20 mg, 0.05 mmol) and 3,5-dihydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1° A TFA in water/CH3CN) to obtain the compound of Example 27 (6.1 mg, 29%).
MS: 312
Intermediate 1 (20 mg, 0.05 mmol) and 3,4,5-trihydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 28 (3.5 mg, 16%).
MS: 328
Intermediate 1 (100 mg, 0.253 mmol) and 2-methyl-4-methoxyphenyl boronic acid (46.1 mg, 0.278 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (14.6 mg, 12.7 μmol) and Na2CO3 (80.5 mg, 0.759 mmol) were added to the solution and then stirred at 90° C. for 2.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain a coupling product (69.7 mg, 63%).
MS: 324
CH2Cl2 (2.0 mL) was added to the coupling product obtained from Step 1 (25 mg, 0.057 mmol) to dissolve, 1.0 mol/L BBr3 dichloromethane solution (0.35 mL, 0.35 mmol) was added to the solution and then stirred at room temperature for 3 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective compound of Example 29 (16.7 mg, 68.9%).
MS: 310
Intermediate 1 (20 mg, 0.05 mmol) and 3,4-methylenedioxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 30 (5.4 mg, 25%).
MS: 324
Intermediate 1 (20 mg, 0.05 mmol) and 3,4-ethylenedioxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 31 (12 mg, 53%).
MS: 338
2-bromobenzaldehyde (500 mg, 2.70 mmol) and malonic acid (562 mg, 5.40 mmol) were dissolved in pyridine (5 mL). Pyrrolidine (19.2 mg, 0.270 mmol) was added to the solution and then stirred at 100° C. for 19.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then white crystals of the objective carboxylic acid (402 mg, 65.5%) were obtained by decantation.
1H-NMR (d-DMSO, 300 MHz) σ 6.57 (d, 2H, J=15.8 Hz), 7.36 (ddd, 1H, J=1.8, 7.6, 7.6 Hz), 7.44 (ddd, 1H, J=1.2, 7.6, 7.6 Hz), 7.71 (dd, 1H, J=1.2, 7.6 Hz), 7.84 (d, 1H, 15.8 Hz). 7.90 (dd, J=1.8, 7.6 Hz)
MS: 241
The carboxylic acid obtained from Step 1 (402 mg, 1.77 mmol) was dissolved in DMF (15 mL), CDI (287 mg, 1.77 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (338 mg, 2.13 mmol) was added to the solution and then stirred for 19.5 hours. Then, after the solvent was eliminated in vacuo, TFA (5 mL) was added to the residue and then stirred at room temperature for 6 hours. The solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (308 mg, 45.5%).
1H-NMR (d-DMSO, 300 MHz) σ 6.78 (d, 1H, J=16 Hz), 7.43 (ddd, 1H, J=1.8, 7.6 7.6 Hz), 7.52 (ddd, 1H, J=1.2, 7.6, 7.6 Hz), 7.73-7.84 (m, 2H), 8.39 (bs, 1H)
MS: 268
The acylguanidine obtained from Step 2 (50 mg, 0.131 mmol) and 4-hydroxyphenyl boronic acid (19.9 mg, 0.144 mmol) was dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (7.58 mg, 6.60 μmol) and Na2CO3 (41.7 mg, 0.393 mmol) were added to the solution and then stirred at 90° C. for 18.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo, purified by reversed phase HPLC (0.1% TFA in water/CH3CN) and then repurified by silica gel column chromatography (amino, CH2Cl2/MeOH) to obtain the compound of Example 32 (4.14 mg, 7.99%).
MS: 282
The Acylguanidine obtained from Step 2 in Example 32 (50 mg, 0.131 mmol) and 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolane-2-yl)phenol (31.7 mg, 0.144 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (7.58 mg, 6.60 μmol) and Na2CO3 (41.7 mg, 0.393 mmol) were added to the solution and then stirred at 90° C. for 18.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 33 (18.2 mg, 7.99%).
1H-NMR (d-DMSO, 300 MHz) σ 6.67-6.77 (m, 2H), 6.81-6.88 (m, 1H), 7.24-7.32 (m, 1H), 7.40 (dd, 1H, J=1.8, 7.0 Hz), 7.47-7.59 (m, 2H), 7.70 (d, 1H, J=16 Hz), 7.77-7.83 (m, 1H), 8.24-8.48 (bs, 4H)
MS: 282
NaH (97.3 mg, 2.43 mmol) was suspended in THF (10 mL) and then cooled to 0° C. 2-phosphono butyric acid triethyl (613 mg, 2.43 mmol) in THF (3 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, 2-bromobenzaldehyde (300 mg, 1.62 mmol) in THF (3 mL) was added thereto in a slow manner and then stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue. The residue obtained was dissolved in THF (10 mL) and MeOH (2 mL), 2 N NaOH (4 mL, 8.0 mmol) was added and then stirred at 50° C. for 8 hours. The solvent was eliminated in vacuo, 2N HCl was added to acidify the solution and then the precipitated crystals were filtrated to obtain white crystals of the objective carboxylic acid (364 mg, 88.4%).
1H-NMR (d-DMSO, 300 MHz) σ 1.13 (t, 3H, J=7.3 Hz), 2.40 (q, 2H, J=7.3 Hz), 7.16-7.39 (m, 4H), 7.63 (d, 1H, J=8.2 Hz), 7.78 (s, 1H)
MS: 255
The carboxylic acid obtained from Step 2 (250 mg, 0.984 mmol) was dissolved in DMF (10 mL), CDI (191 mg, 1.18 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (188 mg, 1.18 mmol) was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (4 mL) was added to the residue at 0° C. and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (228 mg, 56.6%).
MS: 296
The acylguanidine obtained from Step 2 (42.5 mg, 0.104 mmol) and 3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolane-2-yl)phenol (34.3 mg, 0.156 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (6.01 mg, 5.20 μmol) and Na2CO3 (33.1 mg, 0.312 mmol) were added to the solution and then stirred at 90° C. for 21.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 34 (28.3 mg, 64.3%).
1H-NMR (d-DMSO, 300 MHz) σ 1.05 (t, 3H, J=7.3 Hz), 2.44-2.57 (m, 2H), 6.71-6.84 (m, 3H), 7.21-7.30 (m, 2H), 7.41-7.56 (m, 4H), 8.13-8.50 (bs, 4H)
MS: 310
The objective acylguanidine was obtained from 2-bromo-5-methoxybenzaldehyde in the same manner as described for Intermediate 1.
The intermediate obtained from Step 1 (20 mg, 0.05 mmol) and 3-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain a coupling product (5.4 mg, 25%).
MS: 328
The coupling product obtained from Step 2 (10 mg, 0.023 mmol) was dissolved in CH2Cl2 (1 mL), cooled to 0° C., 1.0 mol/L BBr3 dichloromethane solution (0.34 mL, 0.341 mmol) was added thereto and stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 35 (29.1 mg, 79%).
MS: 312
The compound of Example 39 (20 mg, 0.045 mmol) was dissolved in CH2Cl2 (1 mL), cooled to 0° C., 1.0 mol/L BBr3 dichloromethane solution (0.34 mL, 0.341 mmol) was added thereto and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 36 (10.8 mg, 54%).
MS: 328
Intermediate 2 (20 mg, 0.05 mmol) and 3-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 37 (5 mg, 24%).
MS: 310
An acylguanidine which is an intermediate was obtained from 2-bromo-5-methylbenzaldehyde in the same manner as described for Intermediate 1.
The intermediate obtained from Step 1 (20 mg, 0.05 mmol) and 3-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 38 (4.8 mg, 23%).
MS: 310
An acylguanidine which is an intermediate was obtained from 2-bromo-4,5-dimethoxybenzaldehyde in the same manner as described for Intermediate 1.
The intermediate obtained from Step 1 (20 mg, 0.05 mmol) and 3-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 39 (4.9 mg, 21%).
MS: 356
Intermediate 3 (40 mg, 0.08 mmol) and 3-hydroxyphenylboronic acid (18 mg, 0.11 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (6 mg, 5.2 μmol) and Na2CO3 (42 mg, 0.4 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 40 (5.8 mg, 14%).
MS: 388
An acylguanidine which is an intermediate was obtained from 2,5-dibromobenzaldehyde in the same manner as described for Intermediate 1.
The intermediate obtained from Step 1 (40 mg, 0.08 mmol) and 3-chlorophenyl boronic acid (18 mg, 0.11 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (6 mg, 5.2 μmol) and Na2CO3 (42 mg, 0.4 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 41 (3.7 mg, 9%).
MS: 388
An acylguanidine which is an intermediate was obtained from 2-bromo-4-fluorobenzaldehyde in the same manner as described for Intermediate 1.
The intermediate obtained from Step 1 (20 mg, 0.05 mmol) and 3-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 42 (10.8 mg, 51%).
MS: 314
The intermediate obtained from Step 1 in Example 42 (20 mg, 0.05 mmol) and 4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 43 (10.2 mg, 50%).
MS: 314
The intermediate obtained from Step 1 in Example 35 (20 mg, 0.05 mmol) and 4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain a coupling product (4.3 mg, 20%).
MS: 328
The coupling product obtained from Step 1 (10 mg, 0.023 mmol) was dissolved in CH2Cl2 (1 mL), cooled to 0° C., 1.0 mol/L BBr3 dichloromethane solution (0.34 mL, 0.341 mmol) was added thereto and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 44 (29.1 mg, 40%).
MS: 312
The intermediate obtained from Step 1 in Example 39 (20 mg, 0.05 mmol) and 4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 45 (5.1 mg, 22%).
MS: 356
The compound of Example 45 (20 mg, 0.045 mmol) was dissolved in CH2Cl2 (1 mL), cooled to 0° C., 1.0 mol/L BBr3 dichloromethane solution (0.34 mL, 0.341 mmol) was added thereto and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 46 (10.8 mg, 24%).
MS: 328
Intermediate 2 (20 mg, 0.05 mmol) and 4-hydroxyphenyl boronic acid (10 mg, 0.06 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 3 mL). Pd(PPh3)4 (3.00 mg, 2.60 μmol) and Na2CO3 (21.0 mg, 0.2 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 47 (8.1 mg, 38%).
MS: 310
The intermediate obtained from Step 1 in Example 41 and 4-hydroxyphenyl boronic acid were reacted to obtain the compound of Example 48 (7.4 mg, 18%) in the same manner as described in Example 41.
MS: 388
2-fluorobenzaldehyde (100 mg, 0.806 mmol) and 3-methoxyphenol (110 mg, 0.886 mmol) were dissolved in DMA (4 mL), K2CO3 (335 mg, 2.42 mmol) was added thereto and then stirred at 170° C. for 1.5 hours. After cooling it to room temperature, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective aldehyde (110 mg, 59.8%).
1H-NMR (d-DMSO, 300 MHz) σ 3.80 (s, 3H), 6.59-6.66 (m, 2H), 6.73 (ddd, 1H, J=1.2, 2.4, 8.2 Hz), 6.95 (d, 1H, J=8.2 Hz), 7.20 (dd, 1H, J=7.3, 8.5 Hz), 7.28 (dd, 1H, J=8.5, 8.5 Hz), 7.52 (ddd, 1H, J=1.8, 7.3, 8.5 Hz), 7.94 (dd, 1H, J=1.8, 7.9 Hz), 10.5 (s, 1H)
MS: 229
NaH (60% assay, 28.9 mg, 0.723 mmol) was suspended in THF (5 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (182 mg, 0.723 mmol) in THF (2 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, the aldehyde obtained from Step 1 (110 mg, 0.482 mmol) in THF (1 mL) was added thereto in a slow manner and then stirred for 22 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue. The residue obtained was dissolved in THF mL) and MeOH (2 mL), 2N NaOH (2 mL, 4.0 mmol) was added thereto and then stirred at 50° C. for 22 hours. The solvent was eliminated in vacuo, 2 N HCl was added to acidify the solution and then crystals precipitated were filtrated to obtain white crystals of the objective carboxylic acid (114 mg, 83.1%).
MS: 285
The carboxylic acid obtained from Step 2 (114 mg, 0.400 mmol) was dissolved in DMF mL), CDI (77.8 mg, 0.480 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (76.4 mg, 0.480 mmol) was added to the solution and then stirred for approximately 3 days. After the solvent was eliminated in vacuo, TFA (3 mL) was added to the residue and then stirred for 2.5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 49 (97.8 mg, 55.3 W.
MS: 326
2-fluorobenzaldehyde (100 mg, 0.806 mmol) and 4-methoxyphenol (110 mg, 0.886 mmol) were dissolved in DMA (4 mL), K2CO3 (335 mg, 2.42 mmol) was added thereto and then stirred at 170° C. for 2.5 hours. After cooling it to room temperature, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective aldehyde which was a crude product (185 mg).
MS: 243
NaH (60% assay, 48.6 mg, 1.22 mmol) was suspended in THF (5 mL) and then cooled to 0° C. 2-phosphonopropionic acid triethyl (307 mg, 1.22 mmol) in THF (2 mL) was added dropwise in a slow manner to the solution and then stirred for 30 minutes. Then, the aldehyde obtained from Step 1 (185 mg, 0.810 mmol) in THF (1 mL) was added in a slow manner and stirred for 14.5 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then chided over anhydrous MgSO4. The solvent was then eliminated in vacuo to obtain a residue. The resulting residue was dissolved in THF (4 mL) and MeOH (2 mL), 2 N NaOH (2 mL, 4.0 mmol) was added thereto and then stirred at 50° C. for 5 hours. The solvent was eliminated in vacuo, 2 N HCl was added to acidify the solution and then the crystals precipitated were filtrated to obtain white crystals of the objective carboxylic acid (217 mg, 94.1% from Step 1).
MS: 285
The carboxylic acid obtained from Step 2 (100 mg, 0.352 mmol) was dissolved in DMF (3 mL), CDI (68.4 mg, 0.422 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (67.1 mg, 0.422 mmol) was added to the solution and then stirred for approximately 3 days. After the solvent was eliminated in vacuo, TFA (2 mL) was added to the residue at 0° C. and then stirred for 2.5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (52.4 mg, 33.9%).
1H-NMR (d-DMSO, 300 MHz) σ 2.01 (s, 3H), 3.69 (s, 3H), 6.72 (d, 1H, J=8.2 Hz), 6.88-6.97 (m, 4H), 7.12 (dd, 1H, J=7.8, 8.2 Hz), 7.31 (ddd, 1H, J=1.5, 7.8, 8.5 Hz), 7.44 (dd, 1H, J=1.2, 7.8 Hz), 7.58 (s, 1H), 8.11-8.55 (bs, 4H)
MS: 326
The compound obtained from Step 3 (30 mg, 0.0683 mmol) was dissolved in CH2Cl2 (1 mL), cooled to 0° C., 1.0 mol/L BBr3 in dichloromethane (0.34 mL, 0.341 mmol) was added thereto and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 50 (29.1 mg, 100
MS: 312
Intermediate 1 and 2-methanesulfonamidephenyl boronic acid were reacted in the same manner as described in Example 1 to obtain the compound of Example 51.
MS: 373
Intermediate 1 and 2,3-dimethoxyphenyl boronic acid were reacted in the same manner as described in Example 1 to obtain the compound of Example 52.
MS: 340
Intermediate 1 (100 mg, 0.252 mmol) and 2,4-dimethoxyphenyl boronic acid (55.1 mg. 0.303 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (14.6 mg, 12.6 μmol) and Na2CO3 (80.3 mg, 0.757 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (107.2 mg, 94%).
MS: 340
After 1.0 mol/L BBr3 in dichloromethane solution was added to the intermediate obtained from Step 1 (100 mg, 0.221 mmol) at 0° C., the reaction temperature was elevated to 35° C. and then stirred for 6 hours. After cooling it to 0° C., it was diluted with dichloromethane and then saturated sodium hydrate carbonate solution was added to terminate the reaction. After adding saturated saline thereto, it was extracted with acetonitrile, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 53 (79.8 mg, 85%).
MS: 312
4-bromo-2,5-difluoroanisole (223.0 mg, 1.00 mmol) and 2-formylphenyl boronic acid (179.9 mg. 1.20 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 20 mL). Pd(PPh3)4 (57.8 mg, 0.05 mmol) and Na2CO3 (318.0 mg, 3.0 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, dichloromethane was added thereto, washed with water and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective aldehyde (200 mg, 81%).
MS: 249
NaH (60% assay, 96.7 mg, 2.42 mmol) was suspended in THF (8 mL) and then cooled to 0° C. Triethyl-2-phosphonopropionate (576 mg, 2.42 mmol) in THF (2 mL) was added in a slow manner to the suspension. After stirring it for 15 minutes, the intermediate aldehyde obtained from Step 1 (200 mg, 0.806 mmol) in THF (2 mL) was added thereto and then stirred for 1 hour while gradually heating it to room temperature. EtOAc was added to the reaction solution and then washed with NaHCO3 solution, water and saturated saline. After drying it over anhydrous MgSO4, the solvent was eliminated in vacuo to obtain a crude product.
MS: 333
The resulting crude product was then dissolved in a mixed solution of THF and MeOH (v/v=5/3, 16 m). 2 N NaOH (5 mL, 10 mmol) was added to the solution and then stirred at 50° C. for 6 hours. After cooling it to 0° C., 2 N HCl was added to acidify the solution, dichloromethane was added, washed with water and saturated saline and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel column chromatography (Hexane/EtOAc) to obtain the objective carboxylic acid (123 mg, 50%).
MS: 305
The carboxylic acid obtained from Step 2 (123 mg, 0.383 mmol) was dissolved in DMF (5 mL), CDI (74.5 mg, 0.459 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (73.1 mg, 0.459 mmol) was added to the solution and then stirred for 19.5 hours. After the solvent was eliminated in vacuo, TFA (5 mL) was added to the residue and then stirred at 55° C. for 8 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (17.3 mg, 10%).
MS: 346
After 1.0 mol/L BBr3 dichloromethane solution (3.0 mL, 3.0 mmol) was added to the acylguanidine obtained from Step 3 (10 mg, 0.0218 mmol) at 0° C., the reaction temperature was elevated to 35° C. and then stirred for 6 hours. After cooling it to 0° C., it was diluted with dichloromethane and then saturated sodium hydrogen carbonate solution was added thereto to terminate the reaction. After saturated saline was added thereto, it was extracted with acetonitrile, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 54 (2.1 mg, 22%)
MS: 332
3-bromo-benzoic acid (200 mg, 0.995 mmol) and tert-butoxyamine hydrochloride (111 mg, 0.887 mmol) were dissolved in dichloromethane (10 mL), triethylamine (0.34 mL, 2.42 mmol) and EDCI (186 mg, 0.967 mmol) were added to the solution at room temperature and then stirred for 13 hours. Then, dichloromethane was added thereto, washed with water, saturated NH4Cl and saturated saline and then dried over anhydrous MgSO4. Meter the solvent was eliminated in vacuo, it was purified by silica gel column chromatography (SiO2, Hexame/EtOAc) to obtain the objective compound (147 mg, 54.2%).
MS: 273
The compound obtained from Step 1 (147 mg, 0.539 mmol) and 2-formylphenyl boronic acid (147 mg, 0.539 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL), Pd(PPh3)4 (31.2 mg, 27 μmol) and Na2CO3 (171 mg, 1.62 mmol) were added to the solution and then stirred at 90° C. for 3 hours. After cooling it to room temperature, the solvent was eliminated in vacuo, EtOAc was added thereto, the organic layer was washed with saturated NaHCO3 solution and saturated saline and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain a crude product (229 mg).
MS: 298
NaH (60% assay, 97.2 mg, 2.43 mmol) was suspended in THF (6.0 mL) and then cooled to 0° C. Triethyl-2-phosphonopropionate (579 mg, 2.43 mmol) in THF (1.0 mL) was added dropwise in a slow manner to the suspension and then stirred for 15 minutes. Then, the crude product obtained from Step 2 in THF (1.0 mL) was added thereto in a slow manner and then stirred for 3 days while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline, and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain a residue.
The resulting residue was dissolved in THF (3.0 mL) and MeOH (1.0 mL), 2 N NaOH (1.5 mL, 3.0 mmol) was added thereto and then stirred at room temperature for 2 hours.
The solvent was then eliminated in vacuo, 2 N HCl was added to acidify the solution and then the crystals precipitated were filtrated to obtain white crystals of a carboxylic acid which was a crude product (174 mg).
MS: 354
The carboxylic acid obtained from Step 3 (100 mg, 0.283 mmol) was dissolved in DMF (3.0 mL), CDI (55.1 mg, 0.340 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (53.3 mg, 0.340 mmol) was added to the solution and then stirred for 22 hours. After the solvent was eliminated in vacuo, TFA (4.0 mL) was added to the residue and then stirred for 17.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 55 (28.5 mg, 22.3%)
MS: 339
Intermediate 1 (150 mg, 0.379 mmol) and 4-cyanophenyl boronic acid (111 mg. 0.758 mmol) were dissolved in a mixed solution of dioxane and water (v/v=4/1, 5.0 mL). Pd(PPh3)4 (21.9 mg, 19.0 μmol) and Na2CO3 (161 mg, 1.52 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (84.3 mg, 53%).
MS: 305
The intermediate obtained from Step 1 (70 mg, 0.167 mmol) was dissolved in EtOH (1.0 mL). 4 N HCl in dioxane (4.0 mL) was added to the solution and then stirred at room temperature for 48 hours. After the solvent was eliminated in vacuo, it was dissolved in EtOH (1.0 mL), (NH4)2CO3 (161 mg, 1.67 mmol) was added thereto and then stirred at room temperature for 5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 56 (38 mg, 52%)
MS: 322
Intermediate 1 (100 mg, 0.253 mmol) and 2-methyl-4-cyanophenyl boronic acid (61.0 mg. 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107 mg, 1.01 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (90 mg, 80%).
MS: 319
The intermediate obtained from Step 1 (90 mg, 0.208 mmol) was dissolved in EtOH (0.8 mL). 4 N HCl in dioxane (4.0 mL) was added to the solution and then stirred at room temperature for 72 hours. After the solvent was eliminated in vacuo, it was dissolved in EtOH (2.0 mL), (NH4)2CO3 (200 mg, 2.08 mmol) was added thereto and then stirred at room temperature for 5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 57 (70 mg, 60%)
MS: 336
Intermediate 1 (100 mg, 0.253 mmol) and 3-fluoro-4-cyanophenyl boronic acid (62.5 mg, 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107 mg, 1.01 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (84 mg, 76%).
MS: 323
The intermediate obtained from Step 1 (84 mg, 0.192 mmol) was dissolved in EtOH (0.6 mL). 4 N HCl in dioxane (3.0 mL) was added to the solution and then stirred at room temperature for 6 days. After the solvent was eliminated in vacuo, it was dissolved in EtOH (2.0 mL), (NH4)2CO3 (200 mg, 2.08 mmol) was added thereto and then stirred at room temperature for 12 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 58 (38 mg, 35%)
MS: 340
Intermediate 1 (50 mg, 0.126 mmol) and 2-fluoro-4-methoxyphenyl boronic acid (23.6 mg, 0.138 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.29 mg, 6.30 μmol) and Na2CO3 (40.0 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 2.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective coupling product (38.6 mg, 69.5%).
MS: 328
The coupling product obtained from Step 1 (25 mg, 0.0567 mmol) was dissolved in CH2Cl2 (1.0 mL), 1.0 mol/L BBr3 dichloromethane solution (0.42 mL, 0.420 mmol) was added to the solution and then stirred at room temperature for 4.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 59 (22.3 mg, 92.1%).
1H-NMR (d-DMSO, 400 MHz) σ 2.00 (s, 3H), 6.62 (dd, 1H, J=2.6, 12 Hz), 6.67 (dd, 1H, J=2.6, 8.2 Hz), 7.07 (t, 1H, J=8.8 Hz), 7.26 (s, 1H), 7.33-7.41 (m, 1H), 7.43-7.53 (m, 3H), 8.24 (bs, 4H), 10.6 (bs, 1H)
MS: 314
Intermediate 1 (50 mg, 0.126 mmol) and 2-chloro-4-methoxyphenyl boronic acid (25.9 mg, 0.139 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (8.04 mg, 6.95 μmol) and Na2CO3 (41.6 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective coupling product (38.9 mg, 67.6%).
MS: 345
The coupling product obtained from Step 1 (33 mg, 0.0722 mmol) was dissolved in CH2Cl2 (1.0 mL), 1.0 mol/L BBr3 dichloromethane solution (0.50 mL, 0.50 mmol) was added to the solution and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 60 (22.4 mg, 69.9%).
1H-NMR (d-DMSO, 400 MHz) σ 1.96 (d, 3H, J=1.3 Hz), 6.80 (dd, 1H, J=2.5, 8.5 Hz), 6.91 (d, 1H, J=2.5 Hz), 7.07 (d, 1H, J=8.5 Hz), 7.15-7.18 (m, 1H), 7.28-7.33 (m, 1H), 7.44-7.51 (m, 2H), 8.26 (bs, 4H), 10.1 (s, 1H)
MS: 331
Intermediate 1 (50 mg, 0.126 mmol) and 3-methyl-4-methoxyphenyl boronic acid (23.9 mg, 0.139 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (6.94 mg, 6.00 μmol) and Na2CO3 (41.6 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective coupling product (40.5 mg, 73.5%).
MS: 324
The coupling product obtained from Step 1 (30 mg, 0.0671 mmol) was dissolved in CH2Cl2 (1.0 mL), 1.0 mol/L BBr3 dichloromethane solution (0.50 mL, 0.50 mmol) was added to the solution and then stirred at room temperature for 5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 61 (27.2 mg, 95.8%).
1H-NMR (d-DMSO, 400 MHz) σ 2.00 (d, 3H, J=1.2 Hz), 6.81 (d, 1H, J=8.3 Hz), 6.92 (dd, 1H, J=2.2, 8.4 Hz), 7.06 (d, 1H, J=1.7 Hz), 7.34 (d, 1, J=1.2), 7.35-7.38 (m, 4H), 8.22-8.52 (m, 4H), 9.53 (s, 1H)
MS: 310
Intermediate 2 (50 mg, 0.122 mmol) and 3-fluoro-4-hydroxyphenyl boronic acid (22.8 mg, 0.146 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.0 mg, 6.1 μmol) and Na2CO3 (38.8 mg, 0.366 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 62 (10.9 mg, 20%).
MS: 328
Intermediate 2 (50 mg, 0.122 mmol) and 4-fluoro-3-hydroxyphenyl boronic acid (22.8 mg, 0.146 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.0 mg, 6.10 μmol) and Na2CO3 (38.8 mg, 0.366 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 63 (7.9 mg, 15%).
MS: 328
The intermediate obtained from Step 3 in Example 65 and 3-hydroxyphenyl boronic acid were reacted to obtain the compound of Example 64 in the same manner as described in Example 65.
MS: 477
2-bromo-4-fluorobenzaldehyde (500 mg, 2.46 mmol) was dissolved in DMF (50 mL), 4-hydroxybenzenesulfonamide (511 mg, 2.95 mmol) and K2CO3 (408 mg, 2.96 mmol) were added thereto and then stirred at 100° C. for 2 hours. After cooling it to room temperature, EtOAc was added thereto, washed with water and saturated saline and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel chromatography (Hexane/EtOc) to obtain the objective ether (690 mg, 78%).
NaH (60% assay, 116 mg, 2.9 mmol) was suspended in DMF (50 mL) and then triethyl 2-phosphonopropionate (0.611 mg, 2.9 mmol) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, the compound obtained from Step 1 in DMF (3 mL) was added thereto in a slow manner and then stirred for 18 hours. EtOAc was then added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel chromatography (Hexane/EtOAc) to obtain the objective ester (880 mg, 70%). The resulting compound was then dissolved in THF (5 mL) and MeOH (2 mL), 1 N NaOH (8 mL, 8 mmol) was added thereto and then stirred at room temperature for 8 hours. After the solvent was eliminated in vacuo, 2 N HCl was added to acidify the solution and then crystals precipitated were filtrated to obtain the objective carboxylic acid (830 mg, 100%).
The carboxylic acid obtained from Step 2 (830 mg, 2.0 mmol) was dissolved in DMF (10 mL), CDI (375 mg, 2.3 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (453 mg, 2.85 mmol) was added to the solution and then stirred for 16 hours. Then, EtOAc was added thereto, washed with water and saturated saline and then dried over anhydrous MgSO4. TFA (10 mL) was added to the residue and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain an intermediate acylguanidine (410 mg, 35%).
MS: 454
The compound of Example 65 was obtained from the intermediate obtained from Step 3 in the same manner as described in Example 2.
MS: 477
Intermediate 2 (50 mg, 0.122 mmol) and 2-methoxy-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolane-2-yl)phenol (36.6 mg, 0.0146 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.00 mg, 6.10 μmol) and Na2CO3 (38.8 mg, 0.366 mmol) were added to the solution and then stirred at 90° C. for 2.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 66 (1.7 mg, 3%).
MS: 388
2-methyl-3-bromobenzoic acid (1 g, 4.65 mmol) and triethylamine (0.97 mL, 6.78 mmol) were dissolved in THF (20 mL), chloroformic acid ethyl (0.49 mL, 6.11 mmol) was added thereto while cooling it by ice and then stirred for 15 minutes. The precipitate was then eliminated by suction filtration, 1 g of ice and sodium borohydride (260 mg, 6.78 mmol) were added to the resulting filtrate while cooling it by ice and then stirred overnight. It was washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was then eliminated in vacuo to obtain a residue. The resulting residue was dissolved in chloroform (50 mL), manganese dioxide (2 g, 22.5 mmol) was added thereto and then stirred overnight. After filtration, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain an aldehyde (640 mg, 69%).
MS: 199
NaH (60% assay, 193 mg, 4.82 mmol) was suspended in DMF (10 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (1.05 mg, 4.82 mmol) in DMF (10 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, the aldehyde obtained from Step 1 (640 mg, 3.22 mmol) in DMF (3 mL) was added thereto in a slow manner and then stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was then added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue. The resulting residue was then dissolved in THF (10 mL) and MeOH (4 mL), 2 N NaOH (8 mL, 8 mmol) was added thereto and then stirred at room temperature for 8 hours. The solvent was then eliminated in vacuo, 2 N HCl was added to acidify the solution and then the crystals precipitated were filtrated to obtain white crystals of the objective carboxylic acid (600 mg, 93%).
MS: 256
The carboxylic acid obtained from Step 2 (600 mg, 3.0 mmol) was dissolved in DMF (10 mL), CDI (610 mg, 3.8 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (716 mg, 4.5 mmol) was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (10 mL) was added to the residue and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (250 mg, 20%)
MS: 297
The acylguanidine obtained from Step 3 (50 mg, 0.122 mmol) and 4-hydroxyphenyl boronic acid (18.5 mg, 0.134 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (7.06 mg, 6.10 μmol) and Na2CO3 (40.3 mg, 0.366 mmol) were added to the solution and then stirred at 90° C. for 14 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 67 (20.0 mg, 38.8%).
MS: 310
The intermediate obtained from Step 3 in Example 67 (50 mg, 0.122 mmol) and 3-hydroxyphenyl boronic acid (18.5 mg, 0.134 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.06 mg, 4.56 μmol) and Na2CO3 (40.3 mg, 0.366 mmol) were added to the solution and then stirred at 90° C. overnight. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 68 (20.9 mg, 40.5%).
MS: 310
Intermediate 2 and 2,3-dihydroxyphenyl boronic acid were reacted in the same manner as described in Example 1 to obtain the compound of Example 69.
MS: 326
Intermediate 3 (100 mg, 0.210 mmol) and 4-hydroxyphenyl boronic acid (29.1 mg, 0.210 mmol) were dissolved in a mixed solution of dioxane and water (v/v=4/1, 2.5 mL). PdCl2 (dppf) (8.5 mg, 11.0 μmol) and Na2CO3 (89.0 mg, 0.840 mmol) were added to the solution and then stirred at 90° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 70 (85.0 mg).
MS: 376
4-methoxy-phenol (283 mg, 2.28 mmol) and 2-bromo-4-fluorobenzaldehyde (386 mg, 1.90 mmol) were dissolved in DMF (10 mL), K2CO3 (315 mg, 2.28 mmol) was added to the solution and then stirred at 100° C. for 2 hours. Then, the reaction solution was cooled, EtOAc was added thereto, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was then eliminated in vacuo to obtain a residue. NaH (60% assay, 114 mg, 2.85 mmol) was suspended in DMF (10 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (0.62 mL, 2.85 mmol) in DMF (10 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, the resulting residue in DMF (3 mL) was added in a slow manner and then stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was then added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The residue obtained by eliminating the solvent in vacuo was then purified by silica gel column chromatography to obtain an ester (230 mg, 30%).
MS: 392
The resulting ester (230 mg, 0.59 mmol) was dissolved in THF (5 mL) and MeOH (2 mL), 1 N NaOH (4 mL, 4 mmol) was added thereto and then stirred at room temperature for 8 hours. The solvent was then eliminated in vacuo, 2N HCl was added to acidify the solution and then the crystals precipitated were filtrated to obtain white crystals of the objective carboxylic acid (210 mg, 98%).
MS: 364
The carboxylic acid obtained from Step 1 (210 mg, 0.58 mmol) was dissolved in DMF (10 mL), CDI (113 mg, 0.70 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (138 mg, 0.87 mmol) was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (10 mL) was added to the residue at 0° C. and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (120 mg, 52%).
MS: 282
The acylguanidine obtained from Step 2 (50 mg, 0.096 mmol) and 4-hydroxyphenyl boronic acid (14.6 mg, 0.106 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Then, Pd(PPh3)4 (5.55 mg, 4.80 μmol) and Na2CO3 (30.5 mg, 0.288 mmol) were added to the solution and then stirred at 90° C. for 5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the coupling product (34.1 mg, 66.8%).
1H-NMR (d-DMSO, 400 MHz) σ 2.03 (s, 3H), 3.76 (s, 3H), 6.80 (d, 2H, J=8.6 Hz), 6.89 (d, 1H, J=2.7 Hz), 6.94 (dd, 111, J=2.7, 8.6 Hz), 7.00 (d, 2H, J=9.0 Hz), 7.08 (d, 2H, J=8.6 Hz), 7.12 (d, 2H, J=9.0 Hz), 7.27 (s, 1H), 7.45 (d, 1H, J=8.6 Hz), 8.24 (bs, 4H), 9.67 (s, 1H)
MS: 418
The coupling product obtained from Step 3 (30 mg, 0.0564 mmol) was dissolved in CH2Cl2 (1.0 mL), 1.0 mol/L BBr3 dichloromethane solution (0.50 mL, 0.50 mmol) was added thereto and then stirred at room temperature for 3 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 71 (22.5 mg, 77.1%).
1H-NMR (d-DMSO, 400 MHz) σ 2.02 (d, 3H, J=1.2 Hz), 6.79 (d, 2H, J=8.6 Hz), 6.81 (d, 211, J=9.0 Hz), 6.86 (d, 1H, J=2.7 Hz), 6.91 (dd, 1H, J=2.7, 8.6 Hz), 6.98 (d, 2H, J=9.0 Hz), 7.09 (d, 2H, J=8.6 Hz), 7.22 (s, 1H), 7.43 (d, 1H, J=8.6 Hz), 8.33 (bs, 4H), 9.45 (s, 1H), 9.68 (s, 1H)
MS: 404
Intermediate 4 (31 mg, 0.0653 mmol) and 4-hydroxyphenyl boronic acid (21.6 mg, 0.157 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.5 mg, 6.53 μmol) and Na2CO3 (41.5 mg, 0.392 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 72 (14.1 mg, 43%).
MS: 388
NaOMe in MeOH (0.46 mL, 2.28 mmol) was dissolved in DMF (10 mL), K2CO3 (315 mg, 2.28 mmol) was added thereto and stirred for 15 minutes. 2-bromo-4-fluorobenzaldehyde (386 mg, 1.9 mmol) was added to the solution and then stirred at 100° C. for 2 hours. After cooling it to room temperature, EtOAc was added thereto, washed with NaHCO3 solution and saturated saline and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain the objective aldehyde.
MS: 216
NaH (60% assay, 114 mg, 2.85 mmol) was suspended in DMF (10 mL) and then cooled to 0° C. Triethyl-2-phosphonopropionate (0.62 mL, 2.85 mmol) in DMF (5 mL) was added dropwise in a slow manner to the solution and then stirred for 15 minutes. Then, the aldehyde obtained from Step 1 in DMF (3 mL) was added thereto in a slow manner and then stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain a residue. The resulting residue was dissolved in THF (5 mL) and MeOH (2 mL), 1 N NaOH (4 mL, 4 mmol) was added thereto and then stirred at room temperature for 8 hours. Then, the solvent was eliminated in vacuo, 2 N HCl was added to acidify the solution and then the crystals precipitated were filtrated to obtain white crystals of the objective carboxylic acid (90 mg, 17%).
MS: 272
The carboxylic acid obtained from Step 2 (90 mg, 0.33 mmol) was dissolved in DMF (4 CDI (75 mg, 0.45 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (73 mg, 0.45 mmol) was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (10 mL) was added to the residue and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (80 mg, 57.0%).
MS: 313
The acylguanidine obtained from Step 3 (33.8 mg, 0.079 mmol) and 4-hydroxyphenyl boronic acid (36.0 mg, 0.261 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (13.5 mg, 11.7 μmol) and Na2CO3 (75.3 mg, 0.711 mmol) were added to the solution and then stirred at 90° C. for 6 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 73 (11.0 mg, 31.7%).
1H-NMR (d-DMSO, 400 MHz) σ 2.04 (d, 3H, J=1.2 Hz), 3.84 (s, 3H), 6.79-6.86 (m, 3H), 7.00 (dd, 2H, J=2.7, 8.6 Hz), 7.15 (d, 2H, J=8.6 Hz), 7.27 (s, 1H), 7.42 (d, 1H, J=8.6 Hz), 8.38 (bs, 4H), 9.67 (s, 1H)
MS: 326
Intermediate 2 (100 mg, 0.244 mmol) and 3-hydroxy-4-methoxyphenyl boronic acid pinacol ester (73.2 mg, 0.293 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.1 mg, 0.0122 mmol) and Na2CO3 (155.1 mg, 1.463 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 74 (11.6 mg, 10%).
MS: 340
Intermediate 4 (50 mg, 0.105 mmol) and 2,6-dimethyl-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolane-2-yl)-phenol (62.7 mg, 0.253 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (12.2 mg, 0.0105 mmol) and Na2CO3 (133.8 mg, 1.262 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 75 (7.9 mg, 13 W.
MS: 444
Intermediate 4 (50 mg, 0.105 mmol) and 4-fluoro-3-hydroxyphenyl boronic acid (39.4 mg, 0.253 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (12.2 mg, 0.0105 mmol) and Na2CO3 (133.8 mg, 1.262 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 76 (26.4 mg, 47%).
MS: 424
Intermediate 4 (50 mg, 0.105 mmol) and 3-hydroxyphenyl boronic acid (34.8 mg, 0.253 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (12.2 mg, 0.0105 mmol) and Na2CO3 (133.8 mg, 1.262 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 77 (20.9 mg, 40%).
MS: 388
Intermediate 4 (50 mg, 0.105 mmol) and 2-methoxy-4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolane-2-yl)phenol (63.2 mg, 0.253 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). PdCl2 (dppf) CH2Cl2 (8.6 mg, 0.0105 mmol) and Na2CO3 (133.8 mg, 1.262 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 78 (2.1 mg, 4%).
MS: 448
Intermediate 2 (100 mg, 0.244 mmol) and 3-fluoro-4-cyanophenyl boronic acid (60.3 mg, 0.366 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.1 mg, 12.0 μmol) and Na2CO3 (103.5 mg, 0.976 mmol) were added to the solution and then stirred at 80° C. for 6 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (80 mg, 73%).
MS: 337
The intermediate obtained from Step 1 (60 mg, 0.133 mmol) was dissolved in EtOH (0.4 mL). 4 N HCl in dioxane (2.0 mL) was added to the solution and stirred at room temperature for 36 hours. After the solvent was eliminated in vacuo, it was dissolved in EtOH (2.0 mL), (NH4)2CO3 (200 mg, 2.08 mmol) was added thereto and then stirred at room temperature for 5 hours. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 79 (7.0 mg, 11%).
MS: 355
Intermediate 3 (20 mg, 0.042 mmol) and 4-hydroxyphenyl boronic acid (14.6 mg, 0.106 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (5.55 mg, 4.80 μmol) and Na2CO3 (30.5 mg, 0.288 mmol) were added to the solution and then stirred at 90° C. for 5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 80 (6.5 mg, 30%).
MS: 388
NaH (60% assay, 237 mg, 5.92 mmol) was suspended in DMF (50 mL) and then cooled to 0° C. Triethyl 2-phosphonopropionate (1.29 mL, 5.92 mmol) in DMF (20 mL) was added dropwise in a slow manner to the resulting solution and stirred for 15 minutes. Then, 2-bromo-4-trifluoromethylbenzaldehyde (1.00 g, 3.95 mmol) in DMF (5 mL) was added thereto in a slow manner and stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. The solvent was eliminated in vacuo to obtain a residue.
The resulting residue was dissolved in THF (30 mL) and MeOH (20 mL), 1 N NaOH (10 ml, 10 mmol) was added thereto and stirred at room temperature for 8 hours. The solvent was eliminated in vacuo, 2N HCl was added to acidify the solution and then the precipitated crystals were filtrated to obtain white crystals of the objective carboxylic acid (460 mg, 38%).
MS: 310
The carboxylic acid obtained from Step 2 (460 mg, 1.49 mmol) was dissolved in DMF (20 mL), CDI (289 mg, 1.78 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (355 mg, 2.24 mmol) was added to the solution and then stirred for 16 hours. After the solvent was eliminated in vacuo, TFA (10 mL) was added to the residue at 0° C. and then stirred for 1.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective acylguanidine (228 mg, 33%).
MS: 351
The acylguanidine obtained from Step 3 (50 mg, 0.108 mmol) and 4-hydroxyphenyl boronic acid (16.4 mg, 0.119 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (6.24 mg, 5.40 μmol) and Na2CO3 (35.6 mg, 0.324 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 81 (19.2 mg, 37.3%).
1H-NMR (d-DMSO, 400 MHz) σ 1.96 (s, 3H), 6.63 (d, 2H, J=8.5 Hz), 7.21 (d, 3H, J=8.5 Hz), 7.37 (s, 1H), 7.64 (d, 2H, J=8.5 Hz), 7.68 (s, 1H), 7.76 (d, 1H, J=8.5 Hz), 8.36 (bs, 4H), 9.79 (s, 1H)
MS: 364
An intermediate was obtained from 2-bromo-6-fluoro-benzaldehyde in the same manner as described for Intermediate 1.
MS: 300
The intermediate obtained from Step 1 (93 mg, 0.225 mmol) and 4-hydroxyphenyl boronic acid (46.5 mg, 0.337 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (13.0 mg, 11.0 μmol) and Na2CO3 (95.4 mg, 0.90 mmol) were added to the solution and then stirred at 90° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 82 (46.0 mg, 48%).
MS: 314
Intermediate 1 (100 mg, 0.253 mmol) and 4-methylthiophene-3-boronic acid pinacol ester (85.0 mg, 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107.3 mg, 1.012 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 83.
MS: 300
Intermediate 1 (100 mg, 0.253 mmol) and 1-Boc-pyrrole-3-boronic acid pinacol ester (111 mg, 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107.3 mg, 1.012 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo to obtain a residue. The resulting residue was stirred in TFA (5.0 mL) at room temperature for 30 minutes. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 84.
MS: 269
Intermediate 5 (100 mg, 0.253 mmol) and furan-3-boronic acid (43.0 mg, 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107 mg, 1.01 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH-3CN) to obtain the compound of Example 85 (54 mg, 55%).
MS: 270
Intermediate 5 (100 mg, 0.253 mmol) and 2-methyl-4-methoxyphenyl boronic acid (46.1 mg, 0.278 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 12.7 μmol) and Na2CO3 (80.5 mg, 0.759 mmol) were added to the solution and then stirred at 90° C. for 1.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo, EtOAc was added thereto, and the organic phase was washed with saturated NaHCO3 solution, saturated saline and water and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain a residue.
CH2Cl2 (2.0 mL) was added to the residue obtained from Step 1 to dissolve, 1.0 mol/L BBr3 dichloromethane solution (0.50 mL, 0.50 mmol) was added to the solution and then stirred at room temperature for 3 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 86 (22.3 mg, 20.8%).
1H-NMR (d-DMSO, 400 MHz), σ 2.16 (d, 3H, J=1.2 Hz), 2.20 (s, 3H), 6.68 (dd, 1H, J=2.5, 8.3), 6.71 (dd, 1H, J=1.2, 2.2 Hz), 7.05 (d, 1H, J=8.3 Hz), 7.41 (d, 2H, J=8.3 Hz), 7.52 (s, 1H), 7.56 (d, 2H, J=8.3 Hz), 8.39 (bs, 4H), 9.45 (s, 1H), 11.1 (s, 1H)
MS: 310
Intermediate 5 and 3-hydroxyphenyl boronic acid were reacted to obtain the compound of Example 87 in the same manner as described for Example 1.
MS: 296
Intermediate 5 and 2-hydroxyphenyl boronic acid were reacted to obtain the compound of Example 88 in the same manner as described for Example 1.
MS: 296
Intermediate 5 (50 mg, 0.126 mmol) and 4-fluoro-3-hydroxyphenyl boronic acid (21.7 mg, 0.139 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.0 mL). Pd(PPh3)4 (7.23 mg, 6.3 μmol) and Na2CO3 (40.1 mg, 0.378 mmol) were added to the solution and then stirred at 90° C. for 2 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 89 (10.2 mg, 18.9%).
MS: 314
Intermediate 5 (100 mg, 0.253 mmol) and 2-methyl-4-methoxyphenyl boronic acid (46.1 mg, 0.278 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 12.7 μmol) and Na2CO3 (80.5 mg, 0.759 mmol) were added to the solution and then stirred at 90° C. for 1.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo, EtOAc was added thereto, and the organic phase was washed with saturated NaHCO3 solution and saturated saline and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain a residue. CH2Cl2 (2.0 mL) was added to the resulting residue, 1.0 mol/L BBr3 dichloromethane solution (0.50 mL, 0.50 mmol) was added to the solution and then stirred at room temperature for 3 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 90 (22.3 mg, 20.8%).
MS: 310
Intermediate 5 (100 mg, 0.253 mmol) and 2-fluoro-5-methoxuphenyl boronic acid (64.4 mg, 0.379 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107 mg, 1.01 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (112 mg, 100%).
MS: 328
1.0 mol/L BBr3 dichloromethane solution (2.53 mL, 2.53 mmol) was added at 0° C. to the intermediate obtained from Step 1 (112 mg, 0.253 mmol) and then stirred at room temperature for 3 hours. After cooling it to 0° C., it was diluted with dichloromethane and then water was added to terminate the reaction. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 91 (77.0 mg, 70%).
MS: 314
Intermediate 5 (100 mg, 0.253 mmol) and 3-methoxy-4-hydroxyphenyl boronic acid pinacol ester (95.3 mg, 0.381 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.6 mg, 13.0 μmol) and Na2CO3 (107 mg, 1.01 mmol) were added to the solution and then stirred at 80° C. for 6 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (60.0 mg, 54%).
MS: 326
1.0 mol/L BBr3 dichloromethane solution (0.46 mL, 0.46 mmol) was added at 0° C. to the intermediate obtained from Step 1 (20 mg, 0.046 mmol) and then stirred at room temperature for 12 hours. After cooling it to 0° C., it was diluted with dichloromethane and then water was added to terminate the reaction. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 92 (10.0 mg, 51%).
MS: 312
Intermediate 5 (50 mg, 0.127 mmol) and 4-hydroxymethylphenyl boronic acid (29.0 mg, 0.190 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.5 mL). Pd(PPh3)4 (7.50 mg, 7.0 μmol) and Na2CO3 (54.0 mg, 0.51 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 93 (43 mg, 80 V.
MS: 310
NaH (60% assay, 222 mg, 5.55 mmol) was suspended in THF (20 mL), and triethyl-2-phosphonopropionate (1.19 mL, 5.55 mmol) was added dropwise in a slow manner to the solution and then stirred for 30 minutes. Then, 4-bromo-2-(N-morpholino)-benzaldehyde (500 mg, 1.85 mmol) was added in a slow manner and then stirred for 12 hours. EtOAc was then added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. Then, the solvent was eliminated in vacuo to obtain an ester which was a crude product. The resulting compound was dissolved in THF (5 mL) and MeOH (3 mL), 2 N NaOH (3 mL, 16.0 mmol) was added thereto and then stirred at 50° C. for 30 minutes. Then, the solvent was eliminated in vacuo, 2 N HCl was added to acidify the solution and then extracted with dichloromethane. After the solvent was eliminated, it was dried over Na2SO4 to obtain the objective carboxylic acid.
MS: 326
The carboxylic acid obtained from Step 1 was dissolved in DMF (15.0 mL), CDI (360 mg, 2.22 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (355 mg, 2.22 mmol) was added to the solution and then stirred for 12 hours. Then, EtOAc was added thereto, washed with water and saturated saline and then dried over Na2SO4. TFA (15 mL) was added to the residue and then stirred for 1 hour. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain an intermediate (300 mg, 34%).
MS: 367
The intermediate obtained from Step 2 (50.0 mg, 0.104 mmol) and 4-hydroxyphenyl boronic acid (21.5 mg, 0.156 mmol) were dissolved in a mixed solution of dioxane and water (v/v=4/1, 2.5 mL). Pd(PPh3)4 (6.0 mg, 5.0 μmol) and Na2CO3 (44.0 mg, 0.416 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 94 (41.0 mg, 80%).
MS: 381
Intermediate 6 (50.0 mg, 0.122 mmol) and 4-hydroxyphenyl boronic acid (25.2 mg, 0.183 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (7.0 mg, 6.0 μmol) and Na2CO3 (51.7 mg, 0.488 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 95 (31 mg, 60%).
MS: 324
Intermediate 6 (100 mg, 0.244 mmol) and 2-methyl-4-methoxyphenyl boronic acid (60.8 mg, 0.366 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 4.0 mL). Pd(PPh3)4 (14.0 mg, 12.0 μmol) and Na2CO3 (103.4 mg, 0.976 mmol) were added to the solution and then stirred at 80° C. for 12 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the objective intermediate (55 mg, 50%).
MS: 338
1.0 mol/L BBr3 dichloromethane solution (1.10 mL, 1.10 mmol) was added to the intermediate obtained from Step 1 (50 mg, 0.11 mmol) at 0° C. and then stirred at room temperature for 3 hours. After cooling it to 0° C., it was diluted with dichloromethane and then water was added to terminate the reaction. After the solvent was eliminated in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 96 (24.0 mg, 50%).
MS: 324
NaH (60% assay, 944 mg, 23.6 mmol) was suspended in DMF (50 mL) and then cooled to 0° C. 2-(diethoxy-phosphoryl)-propionic acid tert-butyl ester (5.9 mL, 23.6 mmol) in DMF (10 mL) was added dropwise in a slow manner to the resulting solution and stirred for 15 minutes. Then, 2-bromobenzaldehyde (3.5 g, 18.9 mmol) in DMF (3 mL) was added thereto in a slow manner thereto and stirred for 18 hours while gradually heating it from 0° C. to room temperature. EtOAc was added to the reaction solution, washed with water and saturated saline and then dried over anhydrous MgSO4. After the solvent was eliminated in vacuo, it was purified by silica gel column chromatography (SiO2, Hexane/EtOAc) to obtain the objective ester compound (4.84 g, 86%).
MS: 298
The ester obtained from Step 1 (3.59 g, 12 mmol) and 4-hydroxyphenyl boronic acid (2.0 g, 14.5 mmol) were dissolved in a mixed solution of dioxane and water (v/v=3/1, 2.4 mL). Pd(PPh3)4 (168 mg, 145 μmol) and Na2CO3 (2.5 g, 24 mmol) were added to the solution and then stirred at 90° C. for 15.5 hours. After cooling it to room temperature, the solvent was eliminated in vacuo and then it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain a coupling product (1.9 g, 44%).
MS: 241
Dichloromethane (5 mL), HATU (170 mg, 0.46 mmol), triethylamine (0.09 mL, 0.778 mmol) and 2-methyl-2-phenylpropionic acid (67 mg, 0.41 mmol) were added to the coupling product obtained from Step 2 (115 mg, 0.322 mmol) and then stirred overnight at room temperature. After the solvent was eliminated in vacuo, the resulting residue was dissolved in TFA (3.0 mL) at 0° C. and then stirred at room temperature for 2 hours. After the solvent was eliminated in vacuo, ethylacetate was added thereto; and the organic phase was washed with saturated NaHCO3 solution and saturated saline and then dried over anhydrous MgSO4. A crude product was obtained by eliminating the solvent in vacuo. The resulting crude product was then dissolved in DMF (3.0 mL), CDI (38 mg, 0.23 mmol) was added thereto and then stirred at room temperature for 30 minutes. N-Boc-guanidine (48 mg, 0.25 mmol) was added to the solution and then stirred overnight. After the solvent was eliminated in vacuo, TFA (3.0 mL) was added to the residue and then stirred for 4.5 hours. After concentrating the solvent in vacuo, it was purified by reversed phase HPLC (0.1% TFA in water/CH3CN) to obtain the compound of Example 97 (5 mg, 3%).
MS: 442
Structural formulae of the compounds demonstrated in Examples will be shown in Tables 1, 2, 3, 4, 5 and 6.
Compounds according to the present invention were examined for NHE inhibitory activities by using the following methods.
HLF cells (Human hepatoma cell line) were used as cells for the test. After 1×104 cells/well were seeded to a 96-well plate and cultured for 3 days, they were cultured overnight under a serum-free medium. Then, the cells were incubated in a stain solution of Tetramethylammonium (TMA) Buffer (130 mM TMA-C1, 5 mM KCl, 2 mM CaCl2, 1 mM MgSO2, 25 mM glucose, 20 mM HEPES; pH 7.4) containing 40 mM NH4Cl and 1 μg/mL pH-sensitive fluorescent indicator BCECF-AM at 37° C. for 40 minutes and then BCECF was introduced into the cells. After the cells were washed once with TMA Buffer and incubated in TMA+40 mM NH4Cl solution at 37° C. for 15 minutes, the solution was removed and 20 μL/well of TMA Buffer and 10 μL/well of each test compound solution prepared with TMA Buffer were added to each well. The measurements were carried out by adding 200 μL/well of Na Buffer (130 mM, NaCl, 5 mM KCl, 2 mM CaCl2, 1 mM MgSO2, 1 mM NaH2PO4, mM glucose, 20 mM HEPES; pH 7.4) or TMA Buffer (for base measurement), immediately placing it on FlexStation (Molecular Device) and then, after 10 minutes, measuring at the two wavelengths of 505 nm (excitation wavelength)/530 nm (emission wavelength) (measurement value) and 440 nm (excitation wavelength)/530 nm (emission wavelength) (isosbestic point value). A NHE activity was calculated by dividing a measurement value by an isosbestic point value and a NHE1 inhibitory activity was calculated by the following equation.
NHE1 inhibitory activity(%)=100×(1−(Measurement value[addition of each compound]−Base[addition of TMA Buffer])/(Control[addition of Na Buffer]−Base [addition of TMA Buffer]))
Evaluation results of NHE1 inhibitory activities for representative compounds according to the present invention are shown in Table 7.
OK26 cells (OK (opossum kidney) cells in which human NHE3 genes are overexpressed) were used as cells for the test. The measurements were carried out in the same manner as described in Pharmacological Test Example 1 except that the measurement time was 5 minutes (at 37° C.) to calculate NHE3 inhibitory activities.
Evaluation results of NHE3 inhibitory activities for representative compounds according to the present invention are shown in Table 8.
OK26 cells express endogeneous NHE1 (opNHE1). In order to evaluate NHE3 inhibitory activities more precisely, OK26 ND cell lines in which the expression level of opNHE1 is reduced by 90% were established. Measurements of NHE inhibitory activities were carried out by using the established cell lines in the same manner as described in Pharmacological Test Example 1 except that the measurement time was 8 minutes (at 26° C.) to calculate more precise NHE3 inhibitory activities.
Evaluation results of NHE3 inhibitory activities for representative compounds according to the present invention are shown in Table 9.
Evaluations for membrane permeability of the present compound were conducted by using MDCK (MADIN-DARBY Canine Kidney) cells.
1×106 MDCK (MADIN-DARBY Canine Kidney) cells were seeded to each well and cultivated on a trans-well for 4 days (Mixed medium; DMEM:F12=1:1). The trans-well consists of a upper chamber into which cells are seeded and a lower chamber which is separated by a porous membrane and each test compound added into the upper chamber penetrates through the porous membrane to be detected in the lower chamber. The trans-well system has been used as a model for cell membrane permeability.
Buffer solution (pH 6.5) (138 mM NaCl, 2.7 mM KCl, 25 mM D-Glucose, 20 mM MES, 1.25 mM CaCl2, 0.5 mM MgCl2; pH was adjusted with KOH) was added into the upper chamber (Apical side) while Buffer solution (pH 7.4) (138 mM NaCl, 2.7 mM KCl, 25 mM D-Glucose, 20 mM HEPES, 1.25 mM CaCl2, 0.5 mM MgCl2; pH was adjusted with KOH) was added into the lower chamber (basal side). After it was pre-incubated at 37° C. for 20 minutes, 50 μM of each test compound was added thereto and then reacted at 37° C. for 1 hour. The solutions in the upper and lower chambers were collected and the concentrations of each test compound were determined by LC/MS to calculate membrane permeability values (Pm values) by the following equation.
P
m [cm/sec]=(Concentration of each test compound in the basal side×1.5 mL)/(3600 sec×1.12 cm2×Initial concentration of the added compound)
Membrane permeability values (Pm values) of the compounds of Examples 7 and 15 are shown in Table 10.
Continuous administration test by using renal dysfunction model rats was carried out to determine improving effects on renal dysfunction of the present compound.
After unilateral nephrectomy was performed on 7-week-old Wistar rats and the rats were habituated for 1 week, they were divided into 4 groups based on their body weights, Normal group, Vehicle group, 20 mg/kg of the compound of Example 7-administered group and 50 mg/kg of the compound of Example 7-administered group were assigned to 5 rats, respectively. After the rats were habituated in metabolic cages for 4 days, the test compound (the compound of Example 7) dissolved in 0.5% methylcellulose solution was administered via gavage simultaneously with intraperitoneal administration of oleic acid-containing bovine serum albumin (OA-BSA) at a dose of 2 g/animal once daily for 4 days. Vehicles (0.5% methylcellulose solution) were administered to Normal group and Vehicle group instead of the test compound (the compound of Example 7). In addition, no oleic acid-containing bovine serum albumin was administered to Normal group and only unilateral nephrectomy was performed on the Normal group rats. Urine samples were collected from each rat on the last administration day to the next day of the last administration, and blood sampling and autopsy were conducted on the same day. A result for beta 2-microglobulin, which is a marker for tubular damage, after 4 days administration is shown in
| Number | Date | Country | Kind |
|---|---|---|---|
| 2009-230640 | Oct 2009 | JP | national |
| 2010-129190 | Jun 2010 | JP | national |
