Claims
- 1. A compound comprising a chain of at least three rhamnose moieties linked to a lipid moiety and derivatives thereof.
- 2. The compound of claim 1, wherein said chain comprises seven rhamnose moieties.
- 3. The compound of claim 2, wherein said lipid moiety comprises a complex lipid containing an ester linkage.
- 4. A method for preventing a bacterium from participating in formation of a biofilm, said method comprising exposing said bacterium to a biological surfactant.
- 5. The method of claim 4, wherein said biological surfactant is a rhamnolipid compound.
- 6. The method of claim 5, wherein said rhamnolipid compound comprises a chain having at least three rhamnose moieties linked to a lipid moiety.
- 7. The method of claim 6, wherein said rhamnolipid compound comprises a chain of seven rhamnose moieties linked to a lipid moiety.
- 8. The method of claim 7, wherein said bacterium is selected from the group consisting of Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas acidovorans, Pseudomonas alcaligenes, Pseudomonas putida, Pseudomonas syringae, Pseudomonas aureofaciens, Pseudomonas fragi, Fusobacterium nucleatum, Treponema denticola, Porphyromonas gingivalis, Moraxella catarrhalis, Stenotrophomonas maltophilia, Burkholderia cepacia, Aeromonas hydrophilia, Escherichia coli, Citrobacter freundii, Salmonella typhimurium, Salmonella typhi, Salmonella paratyphi, Salmonella enteritidis, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens, Francisella tularensis, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia alcalifaciens, Providencia rettgeri, Providencia stuartii, Acinetobacter calcoaceticus, Acinetobacter haemolyticus, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudo tuberculosis, Yersinia intermedia, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiseptica, Haemophilus influenzae, Haemophilus parainfluenzae, Haemophilus haemolyticus, Haemophilus parahaemolyticus, Pasteurella multocida, Pasteurella haemolytica, Helicobacter pylori, Campylobacter fetus, Campylobacter jejuni, Campylobacter coli, Borrelia burgdorferi, Vibrio cholerae, Vibrio paramaemolyticus, Legionella pneumophila, Listeria monocytogenes, Neisseria gonorrhoeae, Neisseria meningitidis, Gardnerella vaginalis, Bacteroides spp., Clostridium difficile, Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium intracellulare, Mycrobacterium leprae, Corynebacterium diphtheriae, Corynebacterium ulcerans, Streptococcus spp., Enterococcus spp., Desulfvibrio spp., Actinomyces spp., Erwinia spp., Xanthomonas spp., Xylella spp., Clavibacter spp., Desulfomonas spp., Desulfovibrio spp., Desulfococcus spp., Desulfobacter spp., Desulfobulbus spp., Desulfosarcina spp., Deslfuromonas spp., Bacillus spp., Streptomyces spp., Clostridium spp., Rhodococcus spp., Thermatoga spp., Sphingomonas spp., Zymomonas spp., Micrococcus spp., Azotobacter spp., Norcardia spp., Brevibacterium spp., Alcaligenes spp., Microbispora spp., Micromonospora spp., Methylobacterium organophilum, Pseudomonas reptilivora, Pseudomonas carragienovora, Pseudomonas dentificans, Corynebacterium spp., Propionibacterium spp., Xanothomonas spp., Methylobacterium spp., Chromobacterium spp., Saccharopolyspora spp., Actinobacillus spp., Alteromonas spp., Aeronomonas spp., Agrobacterium tumefaciens, Staphylococcus aureus, Staphylococcus epidennidis, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus warneri, Staphylococcus cohnii, Staphylococcus saprophyticus, Staphylococcus capitis, Staphylococcus lugdunensis, Staphylococcus intemedius, Staphylococcus hyicus, Staphylococcus saccharolyticus and Rhizobium spp., and mutant derivatives thereof.
- 9. The method of claim 8, wherein said bacterium is Pseudomonas aeruginosa.
- 10. The method of claim 8, wherein said bacterial organism is selected from Streptococcus spp.
- 11. The method of claim 8, wherein said bacterial organism is Staphylococcus aureus.
- 12. The method of claim 8, wherein said bacterial organism is Staphylococcus epidermis.
- 13. The method of claim 8, wherein said bacterial organism is a coagulase-negative Staphylococcus.
- 14. The method of claim 7, wherein said rhamnolipid compound is derived from supernatant of a closed culture system.
- 15. A biofilm modulating compound identified by the method comprising:
(a) obtaining supernatant from a closed culture system, said system comprising at least one type of a source microorganism; (b) exposing a target organism to said supernatant or an extract thereof; and (c) measuring the level of said activity, wherein the compound is not an acyl homoserine lactone, a microbially-produced hydrolytic enzyme, a Lactobocillus biosurfactant, or an autoinducing compound.
- 16. The compound of claim 15, wherein said compound is purified from supernatant from a closed culture system.
- 17. The compound of claim 15, wherein said compound is synthesized.
- 18. A method of modulating a biofilm, said method comprising exposing a target organism to the compound of claim 15.
- 19. The method of claim 18, wherein said modulating comprises inhibition of biofilm formation.
- 20. The method of claim 18, wherein said modulating comprises disruption of a preexisting biofilm.
- 21. The method of claim 18, wherein said modulating comprises promoting biofilm formation.
- 22. The method of claim 18, wherein said modulating comprises decreasing the viability of cells within said biofilm.
- 23. The method of claim 18, wherein said modulating comprises potentiating the ability of an antimicrobial agent to decrease the viability of cells within said biofilm.
- 24. The method of claim 18, wherein said biofilm comprises at least one target organism selected from the group consisting of archaea, bacteria, fungi, protozoa, and algae.
- 25. The method of claim 24, wherein said organism is a bacterial organism.
- 26. The method of claim 25, wherein said bacterial organism is selected from the group consisting of Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas acidovorans, Pseudomonas alcaligenes, Pseudomonas putida, Pseudomonas syringae, Pseudomonas aureofaciens, Pseudomonas fragi, Fusobacterium nucleatum, Treponema denticola, Porphyromonas gingivalis, Moraxella catarrhalis, Stenotrophomonas maltophilia, Burkholderia cepacia, Aeromonas hydrophilia, Escherichia coli, Citrobacter freundii, Salmonella typhimurium, Salmonella typhi, Salmonella paratyphi, Salmonella enteritidis, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens, Francisella tularensis, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia alcalifaciens, Providencia rettgeri, Providencia stuartii, Acinetobacter calcoaceticus, Acinetobacter haemolyticus, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudo tuberculosis, Yersinia intermedia, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiseptica, Haemophilus influenzae, Haemophilus parainfluenzae, Haemophilus haemolyticus, Haemophilus parahaemolyticus, Pasteurella multocida, Pasteurella haemolytica, Helicobacter pylori, Campylobacter fetus, Campylobacter jejuni, Campylobacter coli, Borrelia burgdorferi, Vibrio cholerae, Vibrio paramaemolyticus, Legionella pneumophila, Listeria monocytogenes, Neisseria gonorrhoeae, Neisseria meningitidis, Gardnerella vaginalis, Bacteroides spp., Clostridium difficile, Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium intracellulare, Mycrobacterium leprae, Corynebacterium diphtheriae, Corynebacterium ulcerans, Streptococcus spp., Enterococcus spp., Desulfvibrio spp., Actinomyces spp., Erwinia spp., Xanthomonas spp., Xylella spp., Clavibacter spp., Desulfomonas spp., Desulfovibrio spp., Desulfococcus spp., Desulfobacter spp., Desulfobulbus spp., Desulfosarcina spp., Deslfuromonas spp., Bacillus spp., Streptomyces spp., Clostridium spp., Rhodococcus spp., Thermatoga spp., Sphingomonas spp., Zymomonas spp., Micrococcus spp., Azotobacter spp., Norcardia spp., Brevibacterium spp., Alcaligenes spp., Microbispora spp., Micromonospora spp., Methylobacterium organophilum, Pseudomonas reptilivora, Pseudomonas carragienovora, Pseudomonas dentificans, Corynebacterium spp., Propionibacterium spp., Xanothomonas spp., Methylobacterium spp., Chromobacterium spp., Saccharopolyspora spp., Actinobacillus spp., Alteromonas spp., Aeronomonas spp., Agrobacterium tumefaciens, Staphylococcus aureus, Staphylococcus epidennidis, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus warneri, Staphylococcus cohnii, Staphylococcus saprophyticus, Staphylococcus capitis, Staphylococcus lugdunensis, Staphylococcus intermedius, Staphylococcus hyicus, Staphylococcus saccharolyticus and Rhizobium spp., and mutant derivatives thereof.
- 27. The method of claim 26, wherein said bacterial organism is Pseudomonas aeruginosa.
- 28. The method of claim 26, wherein said bacterial organism is selected from Streptococcus spp.
- 29. The method of claim 26, wherein said bacterial organism is Staphylococcus aureus.
- 30. The method of claim 26, wherein said bacterial organism is Staphylococcus epidermis.
- 31. The method of claim 25, wherein said bacterial organism is a coagulase-negative Staphylococcus.
- 32. The method of claim 24, wherein said target organism is a fungal organism.
- 33. The method of claim 32, wherein said fungal organism is selected from the group consisting of Absidia spp., Actinomadura madurae, Actinomyces spp., Allescheria boydii, Alternaria spp., Anthopsis deltoidea, Aphanomyces spp., Apophysomyces eleqans, Armillaria spp., Arnium leoporinum, Aspergillus spp., Aureobasidium pullulans, Basidiobolus ranarum, Bipolaris spp., Blastomyces dermatitidis, Botrytis spp., Candida spp., Centrospora spp., Cephalosporium spp., Ceratocystis spp., Chaetoconidium spp., Chaetomium spp., Cladosporium spp., Coccidioides immitis, Colletotrichum spp, Conidiobolus spp., Corynebacterium tenuis, Cryptoporiopsis spp., Cylindrocladium spp., Cryptococcus spp., Cunninghamella bertholletiae, Curvularia spp., Dactylaria spp., Diplodia spp., Epidermophyton spp., Epidermophyton floccosum, Exserophilum spp., Exophiala spp., Fonsecaea spp., Fulvia spp., Fusarium spp., Geotrichum spp., Guignardia spp., Helminthosporium spp., Histoplasma spp., Lecythophora spp., Macrophomina spp., Madurella spp., Magnaporthe spp., Malassezia furfur, Microsporum spp., Monilinia spp., Mucor spp., Mycocentrospora acerina, Nectria spp., Nocardia spp., Oospora spp., Ophiobolus spp., Paecilomyces spp., Paracoccidioides brasiliensis, Penicillium spp., Phaeosclera dematioides, Phaeoannellomyces spp., Phialemonium obovatum, Phialophora spp., Phlyctaena spp., Phoma spp., Phomopsis spp., Phymatotrichum spp., Phytophthora spp., Pythium spp., Piedraia hortai, Pneumocystis carinii, Puccinia spp., Pythium insidiosum, Rhinocladiella aquaspersa, Rhizomucor pusillus, Rhizoctonia spp., Rhizopus spp., Saccharomyces spp., Saksenaea vasifornis, Sarcinomyces phaeomuriformis, Scerotium spp., Sclerotinia spp., Sphaerotheca spp., Sporothrix schenckii, Syncephalastrum racemosum, Taeniolella boppii, Taphrina spp., Thielaviopsis spp., Torulopsosis spp., Trichophyton spp., Trichosporon spp., Ulocladium chartarum, Ustilago spp., Venturia spp., Verticillium spp., Wangiella dernatitidis, Whetxelinia spp., Xylohypha spp., and their synonyms.
- 34. The method of claim 33, wherein said fungal organism is selected from Candida spp.
- 35. A method of potentiating the ability of an antimicrobial agent to decrease the viability of a microorganism within a biofilm, said method comprising exposing said microorganism to the supernatant of a closed culture system or an extract thereof.
- 36. The method of claim 35, wherein said biological surfactant is a rhamnolipid compound.
- 37. The method of claim 36, wherein said rhamnolipid compound comprises a chain having at least three rhamnose moieties linked to a lipid moiety.
- 38. The method of claim 37, wherein said rhamnolipid compound comprises a chain of seven rhamnose moieties linked to a lipid moiety.
- 39. The method of claim 38, wherein said microorganism is a bacterium selected from the group consisting of Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas acidovorans, Pseudomonas alcaligenes, Pseudomonas putida, Pseudomonas syringae, Pseudomonas aureofaciens, Pseudomonas fragi, Fusobactelium nucleatum, Treponema denticola, Porphyromonas gingivalis, Moraxella catarrhalis, Stenotrophomonas maltophilia, Burkholderia cepacia, Aeromonas hydrophilia, Escherichia coli, Citrobacter freundii, Salmonella typhimurium, Salmonella typhi, Salmonella paratyphi, Salmonella enteritidis, Shigella dysenteriae, Shigella flexneri, Shigella sonnei, Enterobacter cloacae, Enterobacter aerogenes, Klebsiella pneumoniae, Klebsiella oxytoca, Serratia marcescens, Francisella tularensis, Morganella morganii, Proteus mirabilis, Proteus vulgaris, Providencia alcalifaciens, Providencia rettgeri, Providencia stuartii, Acinetobacter calcoaceticus, Acinetobacter haemolyticus, Yersinia enterocolitica, Yersinia pestis, Yersinia pseudo tuberculosis, Yersinia intermedia, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiseptica, Haemophilus influenzae, Haemophilus parainfluenzae, Haemophilus haemolyticus, Haemophilus parahaemolyticus, Pasteurella multocida, Pasteurella haemolytica, Helicobacter pylori, Campylobacter fetus, Campylobacter jejuni, Campylobacter coli, Borrelia burgdorferi, Vibrio cholerae, Vibrio paramaemolyticus, Legionella pneumophila, Listeria monocytogenes, Neisseria gonorrhoeae, Neisseria meningitidis, Gardnerella vaginalis, Bacteroides spp., Clostridium difficile, Mycobacterium tuberculosis, Mycobacterium avium, Mycobacterium intracellulare, Mycrobacterium leprae, Corynebacterium diphtheriae, Corynebacterium ulcerans, Streptococcus spp., Enterococcus spp., Desulfvibrio spp., Actinomyces spp., Erwinia spp., Xanthomonas spp., Xylella spp., Clavibacter spp., Desulfomonas spp., Desulfovibrio spp., Desulfococcus spp., Desulfobacter spp., Desulfobulbus spp., Bacillus spp., Streptomyces spp., Clostridium spp., Rhodococcus spp., Thermatoga spp., Sphingomonas spp., Zymomonas spp., Micrococcus spp., Azotobacter spp., Norcardia spp., Brevibacterium spp., Alcaligenes spp., Microbispora spp., Micromonospora spp., Methylobacterium organophilum, Pseudomonas reptilivora, Pseudomonas carragienovora, Pseudomonas dentificans, Corynebacterium spp., Propionibacterium spp., Xanothomonas spp., Methylobacterium spp., Chromobacterium spp., Saccharopolyspora spp., Actinobacillus spp., Alteromonas spp., Aeronomonas spp., Desulfosarcina spp., Deslfuromonas spp., Agrobacterium tumefaciens, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus haemolyticus, Staphylococcus warneri, Staphylococcus cohnii, Staphylococcus saprophyticus, Staphylococcus capitis, Staphylococcus lugdunensis, Staphlyococcus intermedius, Staphylococcus hyicus, Staphylococcus saccharolyticus and Rhizobium spp., and mutant derivatives thereof.
- 40. The method of claim 39, wherein said bacterium is Pseudomonas aeruginosa.
- 41. The method of claim 39, wherein said bacterial organism is selected from Streptococcus spp.
- 42. The method of claim 39, wherein said bacterial organism is Staphylococcus aureus.
- 43. The method of claim 39, wherein said bacterial organism is Staphylococcus epidermis.
- 44. The method of claim 39, wherein said bacterial organism is a coagulase-negative Staphylococcus.
- 45. The method of claim 35, wherein said rhamnolipid compound is derived from supernatant of a closed culture system.
Parent Case Info
[0001] This application claims priority from U.S. Ser. No. 60/236,639, which was filed on Sep. 29, 2000.
STATEMENT AS TO FEDERALLY SPONSORED RESEARCH
[0002] This invention was made with Government support under grant Nos. GM58213 and AI07519 awarded by the NIH. The Government has certain rights in the invention.
Provisional Applications (1)
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Number |
Date |
Country |
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60236639 |
Sep 2000 |
US |