Novel compounds

Abstract

Polypeptides and polynucleotides of the genes set forth in Table I and methods for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing polypeptides and polynucleotides of the genes set forth in Table I in diagnostic assays.

Description

FIELD OF INVENTION

[0001] This invention relates to newly identified polypeptides and polynucleotides encoding such polypeptides, to their use in diagnosis and in identifying compounds that may be agonists, antagonists that are potentially useful in therapy, and to production of such polypeptides and polynucleotides. The polynucleotides and polypeptides of the present invention also relate to proteins with signal sequences which allow them to be secreted extracellularly or membrane-associated (hereinafter often referred collectively as secreted proteins or secreted polypeptides).

BACKGROUND OF THE INVENTION

[0002] The drug discovery process is currently undergoing a fundamental revolution as it embraces “functional genomics”, that is, high throughput genome- or gene-based biology. This approach as a means to identify genes and gene products as therapeutic targets is rapidly superseding earlier approaches based on “positional cloning”. A phenotype, that is a biological function or genetic disease, would be identified and this would then be tracked back to the responsible gene, based on its genetic map position.

[0003] Functional genomics relies heavily on high-throughput DNA sequencing technologies and the various tools of bioinformatics to identify gene sequences of potential interest from the many molecular biology databases now available. There is a continuing need to identify and characterise further genes and their related polypeptides/proteins, as targets for drug discovery.

[0004] Proteins and polypeptides that are naturally secreted into blood, lymph and other body fluids, or secreted into the cellular membrane are of primary interest for pharmaceutical research and development. The reason for this interest is the relative ease to target protein therapeutics into their place of action (body fluids or the cellular membrane). The natural pathway for protein secretion into extracellular space is the endoplasmic reticulum in eukaryotes and the inner membrane in prokaryotes (Palade, 1975, Science, 189, 347; Milstein, Brownlee, Harrison, and Mathews, 1972, Nature New Biol., 239, 117; Blobel, and Dobberstein, 1975, J. Cell. Biol., 67, 835). On the other hand, there is no known natural pathway for exporting a protein from the exterior of the cells into the cytosol (with the exception of pinocytosis, a mechanism of snake venom toxin intrusion into cells). Therefore targeting protein therapeutics into cells poses extreme difficulties.

[0005] The secreted and membrane-associated proteins include but are not limited to all peptide hormones and their receptors (including but not limited to insulin, growth hormones, chemokines, cytokines, neuropeptides, integrins, kallikreins, lamins, melanins, natriuretic hormones, neuropsin, neurotropins, pituitiary hormones, pleiotropins, prostaglandins, secretogranins, selectins, thromboglobulins, thymosins), the breast and colon cancer gene products, leptin, the obesity gene protein and its receptors, serum albumin, superoxide dismutase, spliceosome proteins, 7TM (transmembrane) proteins also called as G-protein coupled receptors, immunoglobulins, several families of serine proteinases (including but not limited to proteins of the blood coagulation cascade, digestive enzymes), deoxyribonuclease I, etc.

[0006] Therapeutics based on secreted or membrane-associated proteins approved by FDA or foreign agencies include but are not limited to insulin, glucagon, growth hormone, chorionic gonadotropin, follicle stimulating hormone, luteinizing hormone, calcitonin, adrenocorticotropic hormone (ACTH), vasopressin, interleukines, interferones, immunoglobulins, lactoferrin (diverse products marketed by several companies), tissue-type plasminogen activator (Alteplase by Genentech), hyaulorindase (Wydase by Wyeth-Ayerst), dornase alpha (Pulmozyme\ by Genentech), Chymodiactin (chymopapain by Knoll), alglucerase (Ceredase by Genzyme), streptokinase (Kabikinase by Pharmacia) (Streptase by Astra), etc. This indicates that secreted and membrane-associated proteins have an established, proven history as therapeutic targets. Clearly, there is a need for identification and characterization of further secreted and membrane-associated proteins which can play a role in preventing, ameliorating or correcting dysfunction or disease, including but not limited to diabetes, breast-, prostate-, colon cancer and other malignant tumors, hyper- and hypotension, obesity, bulimia, anorexia, growth abnormalities, asthma, manic depression, dementia, delirium, mental retardation, Huntington's disease, Tourette's syndrome, schizophrenia, growth, mental or sexual development disorders, and dysfunctions of the blood cascade system including those leading to stroke. The proteins of the present invention which include the signal sequences are also useful to further elucidate the mechanism of protein transport which at present is not entirely understood, and thus can be used as research tools.

SUMMARY OF THE INVENTION

[0007] The present invention relates to particular polypeptides and polynucleotides of the genes set forth in Table I, including recombinant materials and methods for their production. Such polypeptides and polynucleotides are of interest in relation to methods of treatment of certain diseases, including, but not limited to, the diseases set forth in Tables m and V, hereinafter referred to as “diseases of the invention”. In a further aspect, the invention relates to methods for identifying agonists and antagonists (e.g., inhibitors) using the materials provided by the invention, and treating conditions associated with imbalance of polypeptides and/or polynucleotides of the genes set forth in Table I with the identified compounds. In still a further aspect, the invention relates to diagnostic assays for detecting diseases associated with inappropriate activity or levels the genes set forth in Table I. Another aspect of the invention concerns a polynucleotide comprising any of the nucleotide sequences set forth in the Sequence Listing and a polypeptide comprising a polypeptide encoded by the nucleotide sequence. In another aspect, the invention relates to a polypeptide comprising any of the polypeptide sequences set forth in the Sequence Listing and recombinant materials and methods for their production. Another aspect of the invention relates to methods for using such polypeptides and polynucleotides. Such uses include the treatment of diseases, abnormalities and disorders (hereinafter simply referred to as diseases) caused by abnormal expression, production, function and or metabolism of the genes of this invention, and such diseases are readily apparent by those skilled in the art from the homology to other proteins disclosed for each attached sequence. In still another aspect, the invention relates to methods to identify agonists and antagonists using the materials provided by the invention, and treating conditions associated with the imbalance with the identified compounds. Yet another aspect of the invention relates to diagnostic assays for detecting diseases associated with inappropriate activity or levels of the secreted proteins of the present invention.

DESCRIPTION OF THE INVENTION

[0008] In a first aspect, the present invention relates to polypeptides the genes set forth in Table I. Such polypeptides include:

[0009] (a) an isolated polypeptide encoded by a polynucleotide comprising a sequence set forth in the Sequence Listing, herein when referring to polynucleotides or polypeptides of the Sequence Listing, a reference is also made to the Sequence Listing referred to in the Sequence Listing;

[0010] (b) an isolated polypeptide comprising a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;

[0011] (c) an isolated polypeptide comprising a polypeptide sequence set forth in the Sequence Listing;

[0012] (d) an isolated polypeptide having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;

[0013] (e) a polypeptide sequence set forth in the Sequence Listing; and

[0014] (f) an isolated polypeptide having or comprising a polypeptide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polypeptide sequence set forth in the Sequence Listing;

[0015] (g) fragments and variants of such polypeptides in (a) to (f).

[0016] Polypeptides of the present invention are believed to be members of the gene families set forth in Table II. They are therefore of therapeutic and diagnostic interest for the reasons set forth in Tables III and V. The biological properties of the polypeptides and polynucleotides of the genes set forth in Table I are hereinafter referred to as “the biological activity” of polypeptides and polynucleotides of the genes set forth in Table I. Preferably, a polypeptide of the present invention exhibits at least one biological activity of the genes set forth in Table I.

[0017] Polypeptides of the present invention also include variants of the aforementioned polypeptides, including all allelic forms and splice variants. Such polypeptides vary from the reference polypeptide by insertions, deletions, and substitutions that may be conservative or non-conservative, or any combination thereof. Particularly preferred variants are those in which several, for instance from 50 to 30, from 30 to 20, from 20 to 10, from 10 to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1 amino acids are inserted, substituted, or deleted, in any combination.

[0018] Preferred fragments of polypeptides of the present invention include an isolated polypeptide comprising an amino acid sequence having at least 30, 50 or 100 contiguous amino acids from an amino acid sequence set forth in the Sequence Listing, or an isolated polypeptide comprising an amino acid sequence having at least 30, 50 or 100 contiguous amino acids truncated or deleted from an amino acid sequence set forth in the Sequence Listing. Preferred fragments are biologically active fragments that mediate the biological activity of polypeptides and polynucleotides of the genes set forth in Table I, including those with a similar activity or an improved activity, or with a decreased undesirable activity. Also preferred are those fragments that are antigenic or immunogenic in an animal, especially in a human.

[0019] Fragments of a polypeptide of the invention may be employed for producing the corresponding full-length polypeptide by peptide synthesis; therefore, these variants may be employed as intermediates for producing the full-length polypeptides of the invention. A polypeptide of the present invention may be in the form of the “mature” protein or may be a part of a larger protein such as a precursor or a fusion protein. It is often advantageous to include an additional amino acid sequence that contains secretory or leader sequences, pro-sequences, sequences that aid in purification, for instance multiple histidine residues, or an additional sequence for stability during recombinant production.

[0020] Polypeptides of the present invention can be prepared in any suitable manner, for instance by isolation form naturally occurring sources, from genetically engineered host cells comprising expression systems (vide infra) or by chemical synthesis, using for instance automated peptide synthesizers, or a combination of such methods. Means for preparing such polypeptides are well understood in the art.

[0021] In a further aspect, the present invention relates to polynucleotides of the genes set forth in Table I. Such polynucleotides include:

[0022] (a) an isolated polynucleotide comprising a polynucleotide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polynucleotide sequence set forth in the Sequence Listing;

[0023] (b) an isolated polynucleotide comprising a polynucleotide set forth in the Sequence Listing;

[0024] (c) an isolated polynucleotide having at least 95%, 96%, 97%, 98%, or 99% identity to a polynucleotide set forth in the Sequence Listing;

[0025] (d) an isolated polynucleotide set forth in the Sequence Listing;

[0026] (e) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;

[0027] (f) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide set forth in the Sequence Listing;

[0028] (g) an isolated polynucleotide having a polynucleotide sequence encoding a polypeptide sequence having at least 95%, 96%, 97%, 98%, or 99% identity to a polypeptide sequence set forth in the Sequence Listing;

[0029] (h) an isolated polynucleotide encoding a polypeptide set forth in the Sequence Listing;

[0030] (i) an isolated polynucleotide having or comprising a polynucleotide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polynucleotide sequence set forth in the Sequence Listing;

[0031] (j) an isolated polynucleotide having or comprising a polynucleotide sequence encoding a polypeptide sequence that has an Identity Index of 0.95, 0.96, 0.97, 0.98, or 0.99 compared to a polypeptide sequence set forth in the Sequence Listing; and polynucleotides that are fragments and variants of the above mentioned polynucleotides or that are complementary to above mentioned polynucleotides, over the entire length thereof.

[0032] Preferred fragments of polynucleotides of the present invention include an isolated polynucleotide comprising an nucleotide sequence having at least 15, 30, 50 or 100 contiguous nucleotides from a sequence set forth in the Sequence Listing, or an isolated polynucleotide comprising a sequence having at least 30, 50 or 100 contiguous nucleotides truncated or deleted from a sequence set forth in the Sequence Listing.

[0033] Preferred variants of polynucleotides of the present invention include splice variants, allelic variants, and polymorphisms, including polynucleotides having one or more single nucleotide polymorphisms (SNPs).

[0034] Polynucleotides of the present invention also include polynucleotides encoding polypeptide variants that comprise an amino acid sequence set forth in the Sequence Listing and in which several, for instance from 50 to 30, from 30 to 20, from 20 to 10, from 10 to 5, from 5 to 3, from 3 to 2, from 2 to 1 or 1 amino acid residues are substituted, deleted or added, in any combination.

[0035] In a further aspect, the present invention provides polynucleotides that are RNA transcripts of the DNA sequences of the present invention. Accordingly, there is provided an RNA polynucleotide that:

[0036] (a) comprises an RNA transcript of the DNA sequence encoding a polypeptide set forth in the Sequence Listing;

[0037] (b) is a RNA transcript of a DNA sequence encoding a polypeptide set forth in the Sequence Listing;

[0038] (c) comprises an RNA transcript of a DNA sequence set forth in the Sequence Listing; or

[0039] (d) is a RNA transcript of a DNA sequence set forth in the Sequence Listing; and RNA polynucleotides that are complementary thereto.

[0040] The polynucleotide sequences set forth in the Sequence Listing show homology with the polynucleotide sequences set forth in Table II. A polynucleotide sequence set forth in the Sequence Listing is a cDNA sequence that encodes a polypeptide set forth in the Sequence Listing. A polynucleotide sequence encoding a polypeptide set forth in the Sequence Listing may be identical to a polypeptide encoding a sequence set forth in the Sequence Listing or it may be a sequence other than a sequence set forth in the Sequence Listing, which, as a result of the redundancy (degeneracy) of the genetic code, also encodes a polypeptide set forth in the Sequence Listing. A polypeptide of a sequence set forth in the Sequence Listing is related to other proteins of the gene families set forth in Table II, having homology and/or structural similarity with the polypeptides set forth in Table II. Preferred polypeptides and polynucleotides of the present invention are expected to have, inter alia, similar biological functions/properties to their homologous polypeptides and polynucleotides. Furthermore, preferred polypeptides and polynucleotides of the present invention have at least one activity of the genes set forth in Table I.

[0041] Polynucleotides of the present invention may be obtained using standard cloning and screening techniques from a cDNA library derived from mRNA from the tissues set forth in Table IV (see for instance, Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y. (1989)). Polynucleotides of the invention can also be obtained from natural sources such as genomic DNA libraries or can be synthesized using well known and commercially available techniques.

[0042] When polynucleotides of the present invention are used for the recombinant production of polypeptides of the present invention, the polynucleotide may include the coding sequence for the mature polypeptide, by itself, or the coding sequence for the mature polypeptide in reading frame with other coding sequences, such as those encoding a leader or secretory sequence, a pre-, or pro- or prepro-protein sequence, or other fusion peptide portions. For example, a marker sequence that facilitates purification of the fused polypeptide can be encoded. In certain preferred embodiments of this aspect of the invention, the marker sequence is a hexa-histidine peptide, as provided in the pQE vector (Qiagen, Inc.) and described in Gentz et al., Proc Natl Acad Sci USA (1989) 86:821-824, or is an HA tag. A polynucleotide may also contain non-coding 5′ and 3′ sequences, such as transcribed, non-translated sequences, splicing and polyadenylation signals, ribosome binding sites and sequences, that stabilize mRNA.

[0043] Polynucleotides that are identical, or have sufficient identity to a polynucleotide sequence set forth in the Sequence Listing, may be used as hybridization probes for cDNA and genomic DNA or as primers for a nucleic acid amplification reaction (for instance, PCR). Such probes and primers may be used to isolate full-length cDNAs and genomic clones encoding polypeptides of the present invention and to isolate cDNA and genomic clones of other genes (including genes encoding paralogs from human sources and orthologs and paralogs from other species) that have a high sequence similarity to sequences set forth in the Sequence Listing, typically at least 95% identity. Preferred probes and primers will generally comprise at least 15 nucleotides, preferably, at least 30 nucleotides and may have at least 50, if not at least 100 nucleotides. Particularly preferred probes will have between 30 and 50 nucleotides. Particularly preferred primers will have between 20 and 25 nucleotides.

[0044] A polynucleotide encoding a polypeptide of the present invention, including homologs from other species, may be obtained by a process comprising the steps of screening a library under stringent hybridization conditions with a labeled probe having a sequence set forth in the Sequence Listing or a fragment thereof, preferably of at least 15 nucleotides; and isolating full-length cDNA and genomic clones containing the polynucleotide sequence set forth in the Sequence Listing. Such hybridization techniques are well known to the skilled artisan. Preferred stringent hybridization conditions include overnight incubation at 42° C. in a solution comprising: 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH 7.6), 5× Denhardt's solution, 10% dextran sulfate, and 20 microgram/ml denatured, sheared salmon sperm DNA; followed by washing the filters in 0.1×SSC at about 65° C. Thus the present invention also includes isolated polynucleotides, preferably with a nucleotide sequence of at least 100, obtained by screening a library under stringent hybridization conditions with a labeled probe having the sequence set forth in the Sequence Listing or a fragment thereof, preferably of at least 15 nucleotides.

[0045] The skilled artisan will appreciate that, in many cases, an isolated cDNA sequence will be incomplete, in that the region coding for the polypeptide does not extend all the way through to the 5′ terminus. This is a consequence of reverse transcriptase, an enzyme with inherently low “processivity” (a measure of the ability of the enzyme to remain attached to the template during the polymerisation reaction), failing to complete a DNA copy of the mRNA template during first strand cDNA synthesis.

[0046] There are several methods available and well known to those skilled in the art to obtain full-length cDNAs, or extend short cDNAs, for example those based on the method of Rapid Amplification of cDNA ends (RACE) (see, for example, Frohman et al., Proc Nat Acad Sci USA 85, 8998-9002, 1988). Recent modifications of the technique, exemplified by the Marathon (trade mark) technology (Clontech Laboratories Inc.) for example, have significantly simplified the search for longer cDNAs. In the Marathon (trade mark) technology, cDNAs have been prepared from mRNA extracted from a chosen tissue and an ‘adaptor’ sequence ligated onto each end. Nucleic acid amplification (PCR) is then carried out to amplify the “missing” 5′ end of the cDNA using a combination of gene specific and adaptor specific oligonucleotide primers. The PCR reaction is then repeated using ‘nested’ primers, that is, primers designed to anneal within the amplified product (typically an adapter specific primer that anneals further 3′ in the adaptor sequence and a gene specific primer that anneals further 5′ in the known gene sequence). The products of this reaction can then be analyzed by DNA sequencing and a full-length cDNA constructed either by joining the product directly to the existing cDNA to give a complete sequence, or carrying out a separate full-length PCR using the new sequence information for the design of the 5′ primer.

[0047] Recombinant polypeptides of the present invention may be prepared by processes well known in the art from genetically engineered host cells comprising expression systems. Accordingly, in a further aspect, the present invention relates to expression systems comprising a polynucleotide or polynucleotides of the present invention, to host cells which are genetically engineered with such expression systems and to the production of polypeptides of the invention by recombinant techniques. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention.

[0048] For recombinant production, host cells can be genetically engineered to incorporate expression systems or portions thereof for polynucleotides of the present invention. Polynucleotides may be introduced into host cells by methods described in many standard laboratory manuals, such as Davis et al., Basic Methods in Molecular Biology (1986) and Sambrook et al.(ibid). Preferred methods of introducing polynucleotides into host cells include, for instance, calcium phosphate transfection, DEAE-dextran mediated transfection, transvection, micro-injection, cationic lipid-mediated transfection, electroporation, transduction, scrape loading, ballistic introduction or infection.

[0049] Representative examples of appropriate hosts include bacterial cells, such as Streptococci, Staplrylococci, E. coli, Streptomyces and Bacillus subtilis cells; fungal cells, such as yeast cells and Aspergillus cells; insect cells such as Drosophila S2 and Spodoptera Sf9 cells; animal cells such as CHO, COS, HeLa, C127, 3T3, BHK, HEK 293 and Bowes melanoma cells; and plant cells.

[0050] A great variety of expression systems can be used, for instance, chromosomal, episomal and virus-derived systems, e.g., vectors derived from bacterial plasmids, from bacteriophage, from transposons, from yeast episomes, from insertion elements, from yeast chromosomal elements, from viruses such as baculoviruses, papova viruses, such as SV40, vaccinia viruses, adenoviruses, fowl pox viruses, pseudorabies viruses and retroviruses, and vectors derived from combinations thereof, such as those derived from plasmid and bacteriophage genetic elements, such as cosmids and phagemids. The expression systems may contain control regions that regulate as well as engender expression. Generally, any system or vector that is able to maintain, propagate or express a polynucleotide to produce a polypeptide in a host may be used. The appropriate polynucleotide sequence may be inserted into an expression system by any of a variety of well-known and routine techniques, such as, for example, those set forth in Sambrook et al., (ibid). Appropriate secretion signals may be incorporated into the desired polypeptide to allow secretion of the translated protein into the lumen of the endoplasmic reticulum, the periplasmic space or the extracellular environment. These signals may be endogenous to the polypeptide or they may be heterologous signals.

[0051] If a polypeptide of the present invention is to be expressed for use in screening assays, it is generally preferred that the polypeptide be produced at the surface of the cell. In this event, the cells may be harvested prior to use in the screening assay. If the polypeptide is secreted into the medium, the medium can be recovered in order to recover and purify the polypeptide. If produced intracellularly, the cells must first be lysed before the polypeptide is recovered.

[0052] Polypeptides of the present invention can be recovered and purified from recombinant cell cultures by well-known methods including ammonium sulfate or ethanol precipitation, acid extraction, anion or cation exchange chromatography, phosphocellulose chromatography, hydrophobic interaction chromatography, affinity chromatography, hydroxylapatite chromatography and lectin chromatography. Most preferably, high performance liquid chromatography is employed for purification. Well known techniques for refolding proteins may be employed to regenerate active conformation when the polypeptide is denatured during intracellular synthesis, isolation and/or purification.

[0053] Polynucleotides of the present invention may be used as diagnostic reagents, through detecting mutations in the associated gene. Detection of a mutated form of a gene is characterized by the polynucleotides set forth in the Sequence Listing in the cDNA or genomic sequence and which is associated with a dysfunction. Will provide a diagnostic tool that can add to, or define, a diagnosis of a disease, or susceptibility to a disease, which results from under-expression, over-expression or altered spatial or temporal expression of the gene. Individuals carrying mutations in the gene may be detected at the DNA level by a variety of techniques well known in the art.

[0054] Nucleic acids for diagnosis may be obtained from a subject's cells, such as from blood, urine, saliva, tissue biopsy or autopsy material. The genomic DNA may be used directly for detection or it may be amplified enzymatically by using PCR, preferably RT-PCR, or other amplification techniques prior to analysis. RNA or cDNA may also be used in similar fashion. Deletions and insertions can be detected by a change in size of the amplified product in comparison to the normal genotype. Point mutations can be identified by hybridizing amplified DNA to labeled nucleotide sequences of the genes set forth in Table I. Perfectly matched sequences can be distinguished from mismatched duplexes by RNase digestion or by differences in melting temperatures. DNA sequence difference may also be detected by alterations in the electrophoretic mobility of DNA fragments in gels, with or without denaturing agents, or by direct DNA sequencing (see, for instance, Myers et al., Science (1985) 230:1242). Sequence changes at specific locations may also be revealed by nuclease protection assays, such as RNase and S1 protection or the chemical cleavage method (see Cotton et al., Proc Natl Acad Sci USA (1985) 85:4397-4401).

[0055] An array of oligonucleotides probes comprising polynucleotide sequences or fragments thereof of the genes set forth in Table I can be constructed to conduct efficient screening of e.g., genetic mutations. Such arrays are preferably high density arrays or grids. Array technology methods are well known and have general applicability and can be used to address a variety of questions in molecular genetics including gene expression, genetic linkage, and genetic variability, see, for example, M. Chee et al., Science, 274, 610-613 (1996) and other references cited therein.

[0056] Detection of abnormally decreased or increased levels of polypeptide or mRNA expression may also be used for diagnosing or determining susceptibility of a subject to a disease of the invention. Decreased or increased expression can be measured at the RNA level using any of the methods well known in the art for the quantitation of polynucleotides, such as, for example, nucleic acid amplification, for instance PCR, RT-PCR, RNase protection, Northern blotting and other hybridization methods. Assay techniques that can be used to determine levels of a protein, such as a polypeptide of the present invention, in a sample derived from a host are well-known to those of skill in the art. Such assay methods include radio-immunoassays, competitive-binding assays, Western Blot analysis and ELISA assays.

[0057] Thus in another aspect, the present invention relates to a diagnostic kit comprising:

[0058] (a) a polynucleotide of the present invention, preferably the nucleotide sequence set forth in the Sequence Listing, or a fragment or an RNA transcript thereof;

[0059] (b) a nucleotide sequence complementary to that of (a);

[0060] (c) a polypeptide of the present invention, preferably the polypeptide set forth in the Sequence Listing or a fragment thereof; or

[0061] (d) an antibody to a polypeptide of the present invention, preferably to the polypeptide set forth in the Sequence Listing.

[0062] It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise a substantial component. Such a kit will be of use in diagnosing a disease or susceptibility to a disease, particularly diseases of the invention, amongst others.

[0063] The polynucleotide sequences of the present invention are valuable for chromosome localisation studies. The sequences set forth in the Sequence Listing are specifically targeted to, and can hybridize with, a particular location on an individual human chromosome. The mapping of relevant sequences to chromosomes according to the present invention is an important first step in correlating those sequences with gene associated disease. Once a sequence has been mapped to a precise chromosomal location, the physical position of the sequence on the chromosome can be correlated with genetic map data. Such data are found in, for example, V. McKusick, Mendelian Inheritance in Man (available on-line through Johns Hopkins University Welch Medical Library). The relationship between genes and diseases that have been mapped to the same chromosomal region are then identified through linkage analysis (co-inheritance of physically adjacent genes). Precise human chromosomal localisations for a genomic sequence (gene fragment etc.) can be determined using Radiation Hybrid (RH) Mapping (Walter, M. Spillett, D., Thomas, P., Weissenbach, J., and Goodfellow, P., (1994) A method for constructing radiation hybrid maps of whole genomes, Nature Genetics 7, 22-28). A number of RH panels are available from Research Genetics (Huntsville, Ala., USA) e.g. the GeneBridge4 RH panel (Hum Mol Genet 1996 March;5(3):339-46 A radiation hybrid map of the human genome. Gyapay G, Schmitt K, Fizames C, Jones H, Vega-Czarny N, Spillett D, Muselet D, Prud'Homme J F, Dib C, Auffray C, Morissette J, Weissenbach J, Goodfellow P N). To determine the chromosomal location of a gene using this panel, 93 PCRs are performed using primers designed from the gene of interest on RH DNAs. Each of these DNAs contains random human genomic fragments maintained in a hamster background (human/hamster hybrid cell lines). These PCRs result in 93 scores indicating the presence or absence of the PCR product of the gene of interest. These scores are compared with scores created using PCR products from genomic sequences of known location. This comparison is conducted at http://www.genome.wi.mit.edu/.

[0064] The polynucleotide sequences of the present invention are also valuable tools for tissue expression studies. Such studies allow the determination of expression patterns of polynucleotides of the present invention which may give an indication as to the expression patterns of the encoded polypeptides in tissues, by detecting the mRNAs that encode them. The techniques used are well known in the art and include in situ hydridization techniques to clones arrayed on a grid, such as cDNA microarray hybridization (Schena et al, Science, 270, 467-470, 1995 and Shalon et al, Genome Res, 6, 639-645, 1996) and nucleotide amplification techniques such as PCR. A preferred method uses the TAQMAN (Trade mark) technology available from Perkin Elmer. Results from these studies can provide an indication of the normal function of the polypeptide in the organism. In addition, comparative studies of the normal expression pattern of mRNAs with that of mRNAs encoded by an alternative form of the same gene (for example, one having an alteration in polypeptide coding potential or a regulatory mutation) can provide valuable insights into the role of the polypeptides of the present invention, or that of inappropriate expression thereof in disease. Such inappropriate expression may be of a temporal, spatial or simply quantitative nature.

[0065] A further aspect of the present invention relates to antibodies. The polypeptides of the invention or their fragments, or cells expressing them, can be used as immunogens to produce antibodies that are immunospecific for polypeptides of the present invention. The term “immunospecific” means that the antibodies have substantially greater affinity for the polypeptides of the invention than their affinity for other related polypeptides in the prior art.

[0066] Antibodies generated against polypeptides of the present invention may be obtained by administering the polypeptides or epitope-bearing fragments, or cells to an animal, preferably a non-human animal, using routine protocols. For preparation of monoclonal antibodies, any technique which provides antibodies produced by continuous cell line cultures can be used. Examples include the hybridoma technique (Kohler, G. and Milstein, C., Nature (1975) 256:495-497), the trioma technique, the human B-cell hybridoma technique (Kozbor et al., Immunology Today (1983) 4:72) and the EBV-hybridoma technique (Cole et al., Monoclonal Antibodies and Cancer Therapy, 77-96, Alan R. Liss, Inc., 1985).

[0067] Techniques for the production of single chain antibodies, such as those described in U.S. Pat. No. 4,946,778, can also be adapted to produce single chain antibodies to polypeptides of this invention. Also, transgenic mice, or other organisms, including other mammals, may be used to express humanized antibodies.

[0068] The above-described antibodies may be employed to isolate or to identify clones expressing the polypeptide or to purify the polypeptides by affinity chromatography. Antibodies against polypeptides of the present invention may also be employed to treat diseases of the invention, amongst others.

[0069] Polypeptides and polynucleotides of the present invention may also be used as vaccines. Accordingly, in a further aspect, the present invention relates to a method for inducing an immunological response in a mammal that comprises inoculating the mammal with a polypeptide of the present invention, adequate to produce antibody and/or T cell immune response, including, for example, cytokine-producing T cells or cytotoxic T cells, to protect said animal from disease, whether that disease is already established within the individual or not. An immunological response in a mammal may also be induced by a method comprises delivering a polypeptide of the present invention via a vector directing expression of the polynucleotide and coding for the polypeptide in vivo in order to induce such an immunological response to produce antibody to protect said animal from diseases of the invention. One way of administering the vector is by accelerating it into the desired cells as a coating on particles or otherwise. Such nucleic acid vector may comprise DNA, RNA, a modified nucleic acid, or a DNA/RNA hybrid. For use a vaccine, a polypeptide or a nucleic acid vector will be normally provided as a vaccine formulation (composition). The formulation may further comprise a suitable carrier. Since a polypeptide may be broken down in the stomach, it is preferably administered parenterally (for instance, subcutaneous, intramuscular, intravenous, or intra-dermal injection). Formulations suitable for parenteral administration include aqueous and non-aqueous sterile injection solutions that may contain anti-oxidants, buffers, bacteriostats and solutes that render the formulation instonic with the blood of the recipient; and aqueous and non-aqueous sterile suspensions that may include suspending agents or thickening agents. The formulations may be presented in unit-dose or multi-dose containers, for example, sealed ampoules and vials and may be stored in a freeze-dried condition requiring only the addition of the sterile liquid carrier immediately prior to use. The vaccine formulation may also include adjuvant systems for enhancing the immunogenicity of the formulation, such as oil-in water systems and other systems known in the art. The dosage will depend on the specific activity of the vaccine and can be readily determined by routine experimentation.

[0070] Polypeptides of the present invention have one or more biological functions that are of relevance in one or more disease states, in particular the diseases of the invention hereinbefore mentioned. It is therefore useful to identify compounds that stimulate or inhibit the function or level of the polypeptide. Accordingly, in a further aspect, the present invention provides for a method of screening compounds to identify those that stimulate or inhibit the function or level of the polypeptide. Such methods identify agonists or antagonists that may be employed for therapeutic and prophylactic purposes for such diseases of the invention as hereinbefore mentioned. Compounds may be identified from a variety of sources, for example, cells, cell-free preparations, chemical libraries, collections of chemical compounds, and natural product mixtures. Such agonists or antagonists so-identified may be natural or modified substrates, ligands, receptors, enzymes, etc., as the case may be, of the polypeptide; a structural or functional mimetic thereof (see Coligan et al., Current Protocols in Immunology 1(2):Chapter 5 (1991)) or a small molecule. Such small molecules preferably have a molecular weight below 2,000 daltons, more preferably between 300 and 1,000 daltons, and most preferably between 400 and 700 daltons. It is preferred that these small molecules are organic molecules.

[0071] The screening method may simply measure the binding of a candidate compound to the polypeptide, or to cells or membranes bearing the polypeptide, or a fusion protein thereof, by means of a label directly or indirectly associated with the candidate compound. Alternatively, the screening method may involve measuring or detecting (qualitatively or quantitatively) the competitive binding of a candidate compound to the polypeptide against a labeled competitor (e.g. agonist or antagonist). Further, these screening methods may test whether the candidate compound results in a signal generated by activation or inhibition of the polypeptide, using detection systems appropriate to the cells bearing the polypeptide. Inhibitors of activation are generally assayed in the presence of a known agonist and the effect on activation by the agonist by the presence of the candidate compound is observed. Further, the screening methods may simply comprise the steps of mixing a candidate compound with a solution containing a polypeptide of the present invention, to form a mixture, measuring an activity of the genes set forth in Table I in the mixture, and comparing activity of the mixture of the genes set forth in Table I to a control mixture which contains no candidate compound.

[0072] Polypeptides of the present invention may be employed in conventional low capacity screening methods and also in high-throughput screening (HTS) formats. Such HTS formats include not only the well-established use of 96- and, more recently, 384-well micotiter plates but also emerging methods such as the nanowell method described by Schullek et al, Anal Biochem., 246, 20-29, (1997).

[0073] Fusion proteins, such as those made from Fc portion and polypeptide of the genes set forth in Table I, as hereinbefore described, can also be used for high-throughput screening assays to identify antagonists for the polypeptide of the present invention (see D. Bennett et al., J Mol Recognition, 8:52-58 (1995); and K. Johanson et al., J Biol Chem, 270(16):9459-9471 (1995)).

[0074] The polynucleotides, polypeptides and antibodies to the polypeptide of the present invention may also be used to configure screening methods for detecting the effect of added compounds on the production of mRNA and polypeptide in cells. For example, an ELISA assay may be constructed for measuring secreted or cell associated levels of polypeptide using monoclonal and polyclonal antibodies by standard methods known in the art. This can be used to discover agents that may inhibit or enhance the production of polypeptide (also called antagonist or agonist, respectively) from suitably manipulated cells or tissues.

[0075] A polypeptide of the present invention may be used to identify membrane bound or soluble receptors, if any, through standard receptor binding techniques known in the art. These include, but are not limited to, ligand binding and crosslinking assays in which the polypeptide is labeled with a radioactive isotope (for instance, 125I), chemically modified (for instance, biotinylated), or fused to a peptide sequence suitable for detection or purification, and incubated with a source of the putative receptor (cells, cell membranes, cell supernatants, tissue extracts, bodily fluids). Other methods include biophysical techniques such as surface plasmon resonance and spectroscopy. These screening methods may also be used to identify agonists and antagonists of the polypeptide that compete with the binding of the polypeptide to its receptors, if any. Standard methods for conducting such assays are well understood in the art.

[0076] Examples of antagonists of polypeptides of the present invention include antibodies or, in some cases, oligonucleotides or proteins that are closely related to the ligands, substrates, receptors, enzymes, etc., as the case may be, of the polypeptide, e.g., a fragment of the ligands, substrates, receptors, enzymes, etc.; or a small molecule that bind to the polypeptide of the present invention but do not elicit a response, so that the activity of the polypeptide is prevented.

[0077] Screening methods may also involve the use of transgenic technology and the genes set forth in Table I. The art of constructing transgenic animals is well established. For example, the genes set forth in Table I may be introduced through microinjection into the male pronucleus of fertilized oocytes, retroviral transfer into pre- or post-implantation embryos, or injection of genetically modified, such as by electroporation, embryonic stem cells into host blastocysts. Particularly useful transgenic animals are so-called “knock-in” animals in which an animal gene is replaced by the human equivalent within the genome of that animal. Knock-in transgenic animals are useful in the drug discovery process, for target validation, where the compound is specific for the human target. Other useful transgenic animals are so-called “knock-out” animals in which the expression of the animal ortholog of a polypeptide of the present invention and encoded by an endogenous DNA sequence in a cell is partially or completely annulled. The gene knock-out may be targeted to specific cells or tissues, may occur only in certain cells or tissues as a consequence of the limitations of the technology, or may occur in all, or substantially all, cells in the animal. Transgenic animal technology also offers a whole animal expression-cloning system in which introduced genes are expressed to give large amounts of polypeptides of the present invention

[0078] Screening kits for use in the above described methods form a further aspect of the present invention. Such screening kits comprise:

[0079] (a) a polypeptide of the present invention;

[0080] (b) a recombinant cell expressing a polypeptide of the present invention;

[0081] (c) a cell membrane expressing a polypeptide of the present invention; or

[0082] (d) an antibody to a polypeptide of the present invention;

[0083] which polypeptide is preferably that set forth in the Sequence Listing.

[0084] It will be appreciated that in any such kit, (a), (b), (c) or (d) may comprise a substantial component.

[0085] Glossary

[0086] The following definitions are provided to facilitate understanding of certain terms used frequently hereinbefore.

[0087] “Antibodies” as used herein includes polyclonal and monoclonal antibodies, chimeric, single chain, and humanized antibodies, as well as Fab fragments, including the products of an

[0088] Fab or other immunoglobulin expression library.

[0089] “Isolated” means altered “by the hand of man” from its natural state, i.e., if it occurs in nature, it has been changed or removed from its original environment, or both. For example, a polynucleotide or a polypeptide naturally present in a living organism is not “isolated,” but the same polynucleotide or polypeptide separated from the coexisting materials of its natural state is “isolated”, as the term is employed herein. Moreover, a polynucleotide or polypeptide that is introduced into an organism by transformation, genetic manipulation or by any other recombinant method is “isolated” even if it is still present in said organism, which organism may be living or non-living.

[0090] “Secreted protein activity or secreted polypeptide activity” or “biological activity of the secreted protein or secreted polypeptide” refers to the metabolic or physiologic function of said secreted protein including similar activities or improved activities or these activities with decreased undesirable side-effects. Also included are antigenic and immunogenic activities of said secreted protein.

[0091] “Secreted protein gene” refers to a polynucleotide comprising any of the attached nucleotide sequences or allelic variants thereof and/or their complements.

[0092] “Polynucleotide” generally refers to any polyribonucleotide (RNA) or polydeoxribonucleotide (DNA), which may be unmodified or modified RNA or DNA. “Polynucleotides” include, without limitation, single- and double-stranded DNA, DNA that is a mixture of single- and double-stranded regions, single- and double-stranded RNA, and RNA that is mixture of single- and double-stranded regions, hybrid molecules comprising DNA and RNA that may be single-stranded or, more typically, double-stranded or a mixture of single- and double-stranded regions. In addition, “polynucleotide” refers to triple-stranded regions comprising RNA or DNA or both RNA and DNA. The term “polynucleotide” also includes DNAs or RNAs containing one or more modified bases and DNAs or RNAs with backbones modified for stability or for other reasons. “Modified” bases include, for example, tritylated bases and unusual bases such as inosine. A variety of modifications may be made to DNA and RNA; thus, “polynucleotide” embraces chemically, enzymatically or metabolically modified forms of polynucleotides as typically found in nature, as well as the chemical forms of DNA and RNA characteristic of viruses and cells. “Polynucleotide” also embraces relatively short polynucleotides, often referred to as oligonucleotides.

[0093] “Polypeptide” refers to any polypeptide comprising two or more amino acids joined to each other by peptide bonds or modified peptide bonds, i.e., peptide isosteres. “Polypeptide” refers to both short chains, commonly referred to as peptides, oligopeptides or oligomers, and to longer chains, generally referred to as proteins. Polypeptides may contain amino acids other than the 20 gene-encoded amino acids. “Polypeptides” include amino acid sequences modified either by natural processes, such as post-translational processing, or by chemical modification techniques that are well known in the art. Such modifications are well described in basic texts and in more detailed monographs, as well as in a voluminous research literature. Modifications may occur anywhere in a polypeptide, including the peptide backbone, the amino acid side-chains and the amino or carboxyl termini. It will be appreciated that the same type of modification may be present to the same or varying degrees at several sites in a given polypeptide. Also, a given polypeptide may contain many types of modifications. Polypeptides may be branched as a result of ubiquitination, and they may be cyclic, with or without branching. Cyclic, branched and branched cyclic polypeptides may result from post-translation natural processes or may be made by synthetic methods. Modifications include acetylation, acylation, ADP-ribosylation, amidation, biotinylation, covalent attachment of flavin, covalent attachment of a heme moiety, covalent attachment of a nucleotide or nucleotide derivative, covalent attachment of a lipid or lipid derivative, covalent attachment of phosphotidylinositol, cross-linking, cyclization, disulfide bond formation, demethylation, formation of covalent cross-links, formation of cystine, formation of pyroglutamate, formylation, gamma-carboxylation, glycosylation, GPI anchor formation, hydroxylation, iodination, methylation, myristoylation, oxidation, proteolytic processing, phosphorylation, prenylation, racemization, selenoylation, sulfation, transfer-RNA mediated addition of amino acids to proteins such as arginylation, and ubiquitination (see, for instance, Proteins—Structure and Molecular Properties, 2nd Ed., T. E. Creighton, W. H. Freeman and Company, New York, 1993; Wold, F., Post-translational Protein Modifications: Perspectives and Prospects, 1-12, in Post-translational Covalent Modification of Proteins, B. C. Johnson, Ed., Academic Press, New York, 1983; Seifter et al., “Analysis for protein modifications and nonprotein cofactors”, Meth Enzymol, 182, 626-646, 1990, and Rattan et al., “Protein Synthesis: Post-translational Modifications and Aging”, Ann NY Acad Sci, 663,48-62, 1992).

[0094] “Fragment” of a polypeptide sequence refers to a polypeptide sequence that is shorter than the reference sequence but that retains essentially the same biological function or activity as the reference polypeptide. “Fragment” of a polynucleotide sequence refers to a polynucleotide sequence that is shorter than the reference sequence set forth in the Sequence Listing.

[0095] “Variant” refers to a polynucleotide or polypeptide that differs from a reference polynucleotide or polypeptide, but retains the essential properties thereof. A typical variant of a polynucleotide differs in nucleotide sequence from the reference polynucleotide. Changes in the nucleotide sequence of the variant may or may not alter the amino acid sequence of a polypeptide encoded by the reference polynucleotide. Nucleotide changes may result in amino acid substitutions, additions, deletions, fusions and truncations in the polypeptide encoded by the reference sequence, as discussed below. A typical variant of a polypeptide differs in amino acid sequence from the reference polypeptide. Generally, alterations are limited so that the sequences of the reference polypeptide and the variant are closely similar overall and, in many regions, identical. A variant and reference polypeptide may differ in amino acid sequence by one or more substitutions, insertions, deletions in any combination. A substituted or inserted amino acid residue may or may not be one encoded by the genetic code. Typical conservative substitutions include Gly, Ala; Val, Ile, Leu; Asp, Glu; Asn, Gln; Ser, Thr; Lys, Arg; and Phe and Tyr. A variant of a polynucleotide or polypeptide may be naturally occurring such as an allele, or it may be a variant that is not known to occur naturally. Non-naturally occurring variants of polynucleotides and polypeptides may be made by mutagenesis techniques or by direct synthesis. Also included as variants are polypeptides having one or more post-translational modifications, for instance glycosylation, phosphorylation, methylation, ADP ribosylation and the like. Embodiments include methylation of the N-terminal amino acid, phosphorylations of serines and threonines and modification of C-terminal glycines.

[0096] “Allele” refers to one of two or more alternative forms of a gene occurring at a given locus in the genome.

[0097] “Polymorphism” refers to a variation in nucleotide sequence (and encoded polypeptide sequence, if relevant) at a given position in the genome within a population.

[0098] “Single Nucleotide Polymorphism” (SNP) refers to the occurrence of nucleotide variability at a single nucleotide position in the genome, within a population. An SNP may occur within a gene or within intergenic regions of the genome. SNPs can be assayed using Allele Specific Amplification (ASA). For the process at least 3 primers are required. A common primer is used in reverse complement to the polymorphism being assayed. This common primer can be between 50 and 1500 bps from the polymorphic base. The other two (or more) primers are identical to each other except that the final 3′ base wobbles to match one of the two (or more) alleles that make up the polymorphism. Two (or more) PCR reactions are then conducted on sample DNA, each using the common primer and one of the Allele Specific Primers.

[0099] “Splice Variant” as used herein refers to cDNA molecules produced from RNA molecules initially transcribed from the same genomic DNA sequence but which have undergone alternative RNA splicing. Alternative RNA splicing occurs when a primary RNA transcript undergoes splicing, generally for the removal of introns, which results in the production of more than one mRNA molecule each of that may encode different amino acid sequences. The term splice variant also refers to the proteins encoded by the above cDNA molecules.

[0100] “Identity” reflects a relationship between two or more polypeptide sequences or two or more polynucleotide sequences, determined by comparing the sequences. In general, identity refers to an exact nucleotide to nucleotide or amino acid to amino acid correspondence of the two polynucleotide or two polypeptide sequences, respectively, over the length of the sequences being compared.

[0101] “% Identity”—For sequences where there is not an exact correspondence, a “% identity” may be determined. In general, the two sequences to be compared are aligned to give a maximum correlation between the sequences. This may include inserting “gaps” in either one or both sequences, to enhance the degree of alignment. A % identity may be determined over the whole length of each of the sequences being compared (so-called global alignment), that is particularly suitable for sequences of the same or very similar length, or over shorter, defined lengths (so-called local alignment), that is more suitable for sequences of unequal length.

[0102] “Similarity” is a further, more sophisticated measure of the relationship between two polypeptide sequences. In general, “similarity” means a comparison between the amino acids of two polypeptide chains, on a residue by residue basis, taking into account not only exact correspondences between a between pairs of residues, one from each of the sequences being compared (as for identity) but also, where there is not an exact correspondence, whether, on an evolutionary basis, one residue is a likely substitute for the other. This likelihood has an associated “score” from which the “% similarity” of the two sequences can then be determined.

[0103] Methods for comparing the identity and similarity of two or more sequences are well known in the art. Thus for instance, programs available in the Wisconsin Sequence Analysis Package, version 9.1 (Devereux J et al, Nucleic Acids Res, 12, 387-395, 1984, available from Genetics Computer Group, Madison, Wis., USA), for example the programs BESTFIT and GAP, may be used to determine the % identity between two polynucleotides and the % identity and the % similarity between two polypeptide sequences. BESTFIT uses the “local homology” algorithm of Smith and Waterman (J Mol Biol, 147,195-197, 1981, Advances in Applied Mathematics, 2, 482-489, 1981) and finds the best single region of similarity between two sequences. BESTFIT is more suited to comparing two polynucleotide or two polypeptide sequences that are dissimilar in length, the program assuming that the shorter sequence represents a portion of the longer. In comparison, GAP aligns two sequences, finding a “maximum similarity”, according to the algorithm of Neddleman and Wunsch (J Mol Biol, 48, 443-453, 1970). GAP is more suited to comparing sequences that are approximately the same length and an alignment is expected over the entire length. Preferably, the parameters “Gap Weight” and “Length Weight” used in each program are 50 and 3, for polynucleotide sequences and 12 and 4 for polypeptide sequences, respectively. Preferably, % identities and similarities are determined when the two sequences being compared are optimally aligned.

[0104] Other programs for determining identity and/or similarity between sequences are also known in the art, for instance the BLAST family of programs (Altschul S F et al, J Mol Biol, 215, 403-410, 1990, Altschul S F et al, Nucleic Acids Res., 25:389-3402, 1997, available from the National Center for Biotechnology Information (NCBI), Bethesda, Md., USA and accessible through the home page of the NCBI at www.ncbi.nlm.nih.gov) and FASTA (Pearson W R, Methods in Enzymology, 183, 63-99, 1990; Pearson W R and Lipman D J, Proc Nat Acad Sci USA, 85, 2444-2448,1988, available as part of the Wisconsin Sequence Analysis Package).

[0105] Preferably, the BLOSUM62 amino acid substitution matrix (Henikoff S and Henikoff J G, Proc. Nat. Acad. Sci. USA, 89, 10915-10919, 1992) is used in polypeptide sequence comparisons including where nucleotide sequences are first translated into amino acid sequences before comparison.

[0106] Preferably, the program BESTFIT is used to determine the % identity of a query polynucleotide or a polypeptide sequence with respect to a reference polynucleotide or a polypeptide sequence, the query and the reference sequence being optimally aligned and the parameters of the program set at the default value, as hereinbefore described.

[0107] “Identity Index” is a measure of sequence relatedness which may be used to compare a candidate sequence (polynucleotide or polypeptide) and a reference sequence. Thus, for instance, a candidate polynucleotide sequence having, for example, an Identity Index of 0.95 compared to a reference polynucleotide sequence is identical to the reference sequence except that the candidate polynucleotide sequence may include on average up to five differences per each 100 nucleotides of the reference sequence. Such differences are selected from the group consisting of at least one nucleotide deletion, substitution, including transition and transversion, or insertion. These differences may occur at the 5′ or 3′ terminal positions of the reference polynucleotide sequence or anywhere between these terminal positions, interspersed either individually among the nucleotides in the reference sequence or in one or more contiguous groups within the reference sequence. In other words, to obtain a polynucleotide sequence having an Identity Index of 0.95 compared to a reference polynucleotide sequence, an average of up to 5 in every 100 of the nucleotides of the in the reference sequence may be deleted, substituted or inserted, or any combination thereof, as hereinbefore described. The same applies mutatis nutandis for other values of the Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.

[0108] Similarly, for a polypeptide, a candidate polypeptide sequence having, for example, an Identity Index of 0.95 compared to a reference polypeptide sequence is identical to the reference sequence except that the polypeptide sequence may include an average of up to five differences per each 100 amino acids of the reference sequence. Such differences are selected from the group consisting of at least one amino acid deletion, substitution, including conservative and non-conservative substitution, or insertion. These differences may occur at the amino- or carboxy-terminal positions of the reference polypeptide sequence or anywhere between these terminal positions, interspersed either individually among the amino acids in the reference sequence or in one or more contiguous groups within the reference sequence. In other words, to obtain a polypeptide sequence having an Identity Index of 0.95 compared to a reference polypeptide sequence, an average of up to 5 in every 100 of the amino acids in the reference sequence may be deleted, substituted or inserted, or any combination thereof, as hereinbefore described. The same applies mutatis mutantdis for other values of the Identity Index, for instance 0.96, 0.97, 0.98 and 0.99.

[0109] The relationship between the number of nucleotide or amino acid differences and the Identity Index may be expressed in the following equation:

n a <x a −(xa•I),

[0110] in which:

[0111] na is the number of nucleotide or amino acid differences,

[0112] xa is the total number of nucleotides or amino acids in a sequence set forth in the Sequence Listing,

[0113] I is the Identity Index,

[0114] • is the symbol for the multiplication operator, and

[0115] in which any non-integer product of xa and I is rounded down to the nearest integer prior to subtracting it from xa.

[0116] “Homolog” is a generic term used in the art to indicate a polynucleotide or polypeptide sequence possessing a high degree of sequence relatedness to a reference sequence. Such relatedness may be quantified by determining the degree of identity and/or similarity between the two sequences as hereinbefore defined. Falling within this generic term are the terms “ortholog”, and “paralog”. “Ortholog” refers to a polynucleotide or polypeptide that is the functional equivalent of the polynucleotide or polypeptide in another species. “Paralog” refers to a polynucleotideor polypeptide that within the same species which is functionally similar.

[0117] “Fusion protein” refers to a protein encoded by two, often unrelated, fused genes or fragments thereof. In one example, EP-A-0 464 533-A discloses fusion proteins comprising various portions of constant region of immunoglobulin molecules together with another human protein or part thereof. In many cases, employing an immunoglobulin Fc region as a part of a fusion protein is advantageous for use in therapy and diagnosis resulting in, for example, improved pharmacokinetic properties [see, e.g., EP-A 0232 262]. On the other hand, for some uses it would be desirable to be able to delete the Fc part after the fusion protein has been expressed, detected and purified.

[0118] All publications and references, including but not limited to patents and patent applications, cited in this specification are herein incorporated by reference in their entirety as if each individual publication or reference were specifically and individually indicated to be incorporated by reference herein as being fully set forth. Any patent application to which this application claims priority is also incorporated by reference herein in its entirety in the manner described above for publications and references.

TABLE I
			Corresponding
	GSK	Nucleic Acid	Protein
Gene Name	Gene ID	SEQ ID NO's	SEQ ID NO's
sbg1571549-	1571549	SEQ ID NO: 1	SEQ ID NO: 56
cystatin-re		SEQ ID NO: 2	SEQ ID NO: 57
sbg1558434SRCR	1558434	SEQ ID NO: 3	SEQ ID NO: 58
		SEQ ID NO: 4	SEQ ID NO: 59
sbg1546354LRR	1546354	SEQ ID NO: 5	SEQ ID NO: 60
		SEQ ID NO: 6	SEQ ID NO: 61
sbg1555869CABP	1555869	SEQ ID NO: 7	SEQ ID NO: 62
		SEQ ID NO: 8	SEQ ID NO: 63
sbg1518024NKR	1518024	SEQ ID NO: 9	SEQ ID NO: 64
		SEQ ID NO: 10	SEQ ID NO: 65
sbg1525809WNT8	1525809	SEQ ID NO: 11	SEQ ID NO: 66
sbg1519904cdk	1519904	SEQ ID NO: 12	SEQ ID NO: 67
sbg1521449-	1521449	SEQ ID NO: 13	SEQ ID NO: 68
connexin		SEQ ID NO: 14	SEQ ID NO: 69
sbg1522659-	1522659	SEQ ID NO: 15	SEQ ID NO: 70
Siglec		SEQ ID NO: 16	SEQ ID NO: 71
sbg1528389ITI	1528389	SEQ ID NO: 17	SEQ ID NO: 72
sbg1526964ISLR	1526964	SEQ ID NO: 18	SEQ ID NO: 73
sbg1539439TSPK	1539439	SEQ ID NO: 19	SEQ ID NO: 74
sbg1571349lipase	1571349	SEQ ID NO: 20	SEQ ID NO: 75
		SEQ ID NO: 21	SEQ ID NO: 76
sbg1565539-	1565539	SEQ ID NO: 22	SEQ ID NO: 77
aCONNEXIN
sbg1565539-	1565539	SEQ ID NO: 23	SEQ ID NO: 78
bCONNEXIN
sbg1563294-	1563294	SEQ ID NO: 24	SEQ ID N0: 79
acadherin		SEQ ID NO: 25	SEQ ID NO: 80
sbg1563294-	1563294	SEQ ID NO: 26	SEQ ID NO: 81
bcadherin
sbg1563509ECM	1563509	SEQ ID NO: 27	SEQ ID NO: 82
		SEQ ID NO: 28	SEQ ID NO: 83
sbg1552159-	1552159	SEQ ID NO: 29	SEQ ID NO: 84
Serprotease
sbg1551159-	1551159	SEQ ID NO: 30	SEQ ID NO: 85
thymosinb4
SBh1686954.SOCS	1686954	SEQ ID NO: 31	SEQ ID NO: 86
sbg1548844slit	1548844	SEQ ID NO: 32	SEQ ID NO: 87
sbg1545249GGT	1545249	SEQ ID NO: 33	SEQ ID NO: 88
		SEQ ID NO: 34	SEQ ID NO: 89
SBh1813899.Y82017	1813899	SEQ ID NO: 35	SEQ ID NO: 90
sbg1536324mapkkk	1536324	SEQ ID NO: 36	SEQ ID NO: 91
gsk1810944BrCaAg	1810944	SEQ ID NO: 37	SEQ ID NO: 92
sbg1535914AIP	1535914	SEQ ID NO: 38	SEQ ID NO: 93
		SEQ ID NO: 39	SEQ ID NO: 94
sbg1530894aCE	1530894	SEQ ID NO: 40	SEQ ID NO: 95
		SEQ ID NO: 41	SEQ ID NO: 96
sbg1530894bCE	1530894	SEQ ID NO: 42	SEQ ID NO: 97
gsk.1813134.NaI	1813134	SEQ ID NO: 43	SEQ ID NO: 98
		SEQ ID NO: 44	SEQ ID NO: 99
gsk1811484BrCaAg	1811484	SEQ ID NO: 45	SEQ ID NO: 100
sbg1529984-	1529984	SEQ ID NO: 46	SEQ ID NO: 101
aSproteinase		SEQ ID NO: 47	SEQ ID NO: 102
sbg1529984-	1529984	SEQ ID NO: 48	SEQ ID NO: 103
bSproteinase
SBGKIN113	575482	SEQ ID NO: 49	SEQ ID NO: 104
gsk305961GDNa	305961	SEQ ID NO: 50	SEQ ID NO: 105
gsk2402719—	2402719	SEQ ID NO: 51	SEQ ID NO: 106
2395124BIG2	and
	2395124
sbg18525LRRb	18525	SEQ ID NO: 52	SEQ ID NO: 107
SBhACRP30c	34718	SEQ ID NO: 53	SEQ ID NO: 108
sbg123493SLITc	123493	SEQ ID NO: 54	SEQ ID NO: 109
sbg102200MCTc	102200	SEQ ID NO: 55	SEQ ID NO: 110

[0119]

TABLE II
		Closest		Cell
		Polynuclotide by	Closest Polypeptide by	Localization
Gene Name	Gene Family	homology	homology	(by homology)
sbg1571549cystatin-re	Testatin	SC: AL121894	Human cystatin-related	Secreted
		Direct submitted	protein, designated
		(25 OCT. 2000) by	testatin,
		Sanger Centre,	geneseqp: Y53771
		Hinxton,	Filed by (KARO-)
		Cambridgeshire,	KAROLINSKA
		CB10 1SA, UK.	INNOVATIONS AB
			Publication number and
			filing date:
			WO9958565-A1, 18
			NOV. 1999
sbg1558434SRCR	Scavenger	GB: AL160313	Human KIAA1822	Membrane-
	receptor with	Directly submitted	protein, gi: 14017860	bound
	cysteine-rich	(23 MAY 2000) by	Submitted (27 MAR.
	domain	Genoscope to the	2001) by Osamu Ohara,
		EMBL/GenBank/	Kazusa DNA Rsearch
		DDBJ databases	Institute, Department of
			Human Gene Research;
			1532-3, Yana,
			Kisarazu, Chiba 292-
			0812, Japan
sbg1546354LRR	Leucine-rich	SC: AL353746	Human neuronal	Membrane-
	repeat (LRR)	Directly submitted	leucine-rich repeat	bound
	protein	(07 SEP. 2000) by	protein, gi: 12309630
		Sanger Centre,	Submitted (17 JAN.
		Hinxton,	2001) by Sanger
		Cambridgeshire,	Centre, Hinxton,
		CB10 1SA, UK	Cambridgeshire, CB10
			1SA, UK.
sbg1555869CABP	S-100 CABP	GB: AC005921	Human S100 calcium-	Cytosolic
		Direct submitted	binding protein A10,
		(03 NOV. 1998)	gi: 4506761
		Whitehead	Kube, E., Weber, K. and
		Institute/MIT Center	Gerke, V. 1991. Gene
		for Genome	102:255-259.
		Research, 320
		Charles Street,
		Cambridge, MA
		02141, USA
sbg1518024NKR	NK-receptor	GB: AL133414	Human natural killer	Membrane-
		Direct submitted (23	cell inhibitory receptor	bound
		MAR. 2000) Sanger	KIR2DL3, gi: 2760897
		Centre, Hinxton,	Direct submitted (02
		Cambridgeshire,	SEP. 1997) Structural
		CB10 1SA, UK	Biology, Stanford
			University, Sherman
			Fairchild Building,
			Stanford University
			School of Medicine,
			Stanford, CA 94305-
			5400, USA.
sbg1525809WNT8	WNT-8	GB: AC004826	Human WNT8A,	Secreted
	protein	Direct submitted	gi: 14495176
		(07 OCT. 2000)	Saitoh, T. and Katoh, M.
		Department of	Int. J. Oncol. 19 (1),
		Genetics,	123-127 (2001)
		Washington
		University, 4444
		Forest Park Avenue,
		St. Louis, Missouri
		63108, USA.
sbg1519904cdk	Cyclin-	EMBL: AL359916	Human cell division	Cytosolic
	dependent	Directly submitted	protein kinase4
	kinase	(29 SEP. 2000) by	(CDK4), gi: 12830335
		Sanger Centre,	Directly submitted (08
		Hinxton,	FEB. 2001) by Sanger
		Cambridgeshire,	Centre, Hinxton,
		CB10 1SA, UK	Cambridgeshire, CB10
			1SA, UK
sbg1521449connexin	Connexin	EMBL: AC004977	Human transport	Membrane-
	protein	Submitted (09	protein TPPT-31,	bound
		MAY 2001)	geneseqp: B60111
		Department of	Filed by (INCY-)
		Genetics,	INCYTE GENOMICS
		Washington	INC
		University, 4444	Publication number and
		Forest Park Avenue,	filing date:
		St. Louis, Missouri	WO200078953-A2,
		63108, USA	28 DEC. 2000
sbg1522659Siglec	Sialic acid-	GB: AC011452	Human sialic acid-	Membrane-
	binding Ig-	Directly submitted	binding lectin 11, gi:	bound
	like lectin	(07 OCT. 1999) by	14718451
	protein	DOE Joint Genome	Submitted (17 JAN.
		Institute, 2800	2001) Glycobiology
		Mitchell Drive,	Research and Training
		Walnut Creek,	Center, University of
		CA 94598, USA	California, San Diego,
			9500 Gilman Drive, La
			Jolla, CA 92093-0687,
			USA
sbg1528389ITI	Kunitz-type	SC: AL050348	Human novel protein	Secreted
	protease	Directly submitted	with Kunitz/Bovine
	inhibitor	(27 NOV. 2000) by	pancreatic trypsin
		Sanger Centre,	inhibitor domain,
		Hinxton,	gi: 13559983,
		Cambridgeshire,	Submitted (04 APR.
		CB10 1SA, UK	2001) by Sanger
			Centre, Hinxton,
			Cambridgeshire, CB10
			1SA, UK.
sbg1526964ISLR	Immunoglobulin	GB: AP001065	Human leucine-rich	Secreted
	superfamily	Submitted (12 JAN.	repeat-containing 3, gi:
	containing	2000) to the	13562088
	leucine-rich	DDBJ/EMBL/GenBank	Submitted (27 MAR.
	repeat	databases by	2001) by Yoonsoo
		Nobuyoshi Shimizu,	Hahn, Korea Research
		Keio University,	Institute of Bioscience
		School of Medicine,	& Biotechnology,
		Molecular Biology;	Genome Research
		35 Shinanomachi,	Center; 52 Eoeun-dong,
		Shinjuku-ku, Tokyo	Yuseong-gu, Taejon
		160-8582, Japan	305-333, South Korea
sbg1539439TSPK	Ser/Thr	EMBL: AC008476	Human serine/threonine	Cytosolic
	protein kinase	Directly submitted	kinase FKSGS1, gi:
		(03 AUG. 1999) by	14042947
		Production	Submitted (07 FEB.
		Sequencing Facility,	2001) Beijing
		DOE Joint Genome	Fengkesheng Function
		Institute, 2800	Gene Technology Ltd.,
		Mitchell Drive,	4 Tou Tiao Lu Chang
		Walnut Creek, CA	Street, Xuanwu
		94598, USA.	District, Beijing
			100050, P. R. China
sbg1571349lipase	Esterase and	EMBL: AC020549	Human membrane	Membrane-
	phospholipase	Directly submitted	associated protein	bound
		(08 NOV. 2000) by	MEMAP-15.
		Department of	geneseqp: B74709
		Genetics,	Filed by (INCY-)
		Washington	INCYTE GENOMICS
		University, 4444	INC
		Forest Park Avenue,	Publication number and
		St. Louis, Missouri	filing date:
		63108, USA	WO200112662-A2,
			22 FEB. 2001
sbg1565539aCONNEXIN	Connexin	SC: AL121749	Human FZD8 gene	Membrane-
		Directly submitted	product (frizzled	bound
		(27 APR. 2000) by	(Drosophila) homolog
		Sanger Centre,	8), gi: 10334641
		Hinxton,	Directly submitted (27
		Cambridgeshire,	APR. 2000) by Sanger
		CB10 1SA, UK	Centre, Hinxton,
			Cambridgeshire, CB10
			1SA, UK
sbg1565539bCONNEXIN	Connexin	SC: AL121749	Human FZD8 gene	Membrane-
		Directly submitted	product (frizzled	bound
		(27 APR. 2000) by	(Drosophila) homolog
		Sanger Centre,	8), gi: 10334641
		Hinxton,	Directly submitted (27
		Cambridgeshire,	APR. 2000) by Sanger
		CB10 1SA, UK	Centre, Hinxton,
			Cambridgeshire, CB10
			1SA, UK
sbg1563294acadherin	Cadherin	SC: AL109928	Human cadherin-like	Secreted
		Submitted (28	protein VR20, isoform
		MAR. 2001) Sanger	1, gi: 7981305
		Centre, Hinxton,	Submitted (28 MAR.
		Cambridgeshire,	2001) Sanger Centre,
		CB10 1SA, UK.	Hinxton,
			Cambridgeshire, CB10
			1SA, UK.
sbg1563294bcadherin	Cadherin	SC: AL109928	Human cadherin-like	Secreted
		Submitted (28	protein VR20, isoform
		MAR. 2001) Sanger	3, gi: 12743872
		Centre, Hinxton,	Submitted (28 MAR.
		Cambridgeshire,	2001) Sanger Centre,
		CB10 1SA, UK.	Hinxton,
			Cambridgeshire, CB10
			1SA, UK.
sbg1563509ECM	Hemicentin	SC: AL133553,	Human hemicentin,	Secreted
		SC: AL135796,	gi: 14575679
		SC: AL121996,	Submitted (02 JUN.
		EMBL: AL135797,	1999) by National
		GB: AL391827	Human Genome
		Submitted by	Research Institute,
		Sanger Centre,	National Institutes of
		Hinxton,	Health, 49 Convent
		Cambridgeshire,	Drive, Building 49
		CB10 1SA, UK on	Room 4A22, Bethesda,
		26 JUL. 2000, 18	MD 20892, USA
		JUL. 2000, 15
		MAY 2001, 31
		OCT. 2000 and 09
		MAY 2001
		respectively.
		SC: AL118582,
		Ressourcenzentrum,
		Heubnerweg 6,
		14059 Berlin-
		Charlottenburg,
		GERMANY
sbg1552159Serprotease	Serine	EMBL: AC012151	Xenopus laevis	Membrane-
	protease	submitted (19 SEP.	polyprotein, gi:	bound
		2000) by Human	7512178
		Genome Sequencing	Submitted (?? MAR.
		Center, Department	1998) to the EMBL
		of Molecular and	Data Library by Yang,
		Human Genetics,	J. C., Lindsay, L. L. and
		Baylor College of	Hedrick, J. L.
		Medicine, One
		Baylor Plaza,
		Houston, TX 77030.
		USA
sbg1551159thymosinb4	Thymosin	GB: AC006312	Mouse thymosin beta 4,	Secreted
	beta4	Submitted (13 APR.	gi: 10946578
		1999) by Whitehead	Rudin CM, Engler P,
		Institute/MIT Center	Storb U. J Immunol
		for Genome	1990 Jun.
		Research, 320	15; 144(12):4857-62
		Charles Street,
		Cambridge, MA
		02141, USA
SBH1686954.SOCS	Cytokine	GS: R-661F4 found	Mouse SOCS14 protein	Cytosolic
	signalling	at Genoscope	geneseqp: W62625
	protein		Filed by (HALL-)
			HALL INST
			MEDICAL RES
			WALTER & ELIZA
			Publication number and
			filing date:
			WO9820023-A1,
			14 MAY 1998
sbg1548844slit	Slit	GB: AC004152	Human membrane	Membrane-
		Submitted (18 FEB.	associated protein	bound
		1998) by Joint	MEMAP-11.
		Genome Institute,	geneseqp: B74705
		Lawrence Livermore	Filed by (INCY-)
		National Laboratory,	Incyte Genomics Inc.
		7000 East Ave.,	Publication number and
		Livermore, CA	filing date:
		94551, USA	WO200112662-A2
			22 FEB. 2001
sbg1545249GGT	Gamma-	EMBL: AC023491	Human glutamyl	Membrane-
	glutamyltrans-	found at European	transpeptidase,	bound
	peptidase	Molecular Biology	gi: 4885271
		Laboratory	Hum. Genet. 76 (3),
			283-286 (1987).
SBh1813899.Y82017	Ankyrin	GB: AL512624	Human immunogenic	Cytosolic
	motif protein	Submitted (12 FEB.	prostate tumour protein,
		2001) by	geneseqp: Y82017
		Genoscope - Centre	Filed by (CORI-)
		National de	CORIXA CORP
		Sequencage: BP	Publication number and
		191 91006 EVRY	filing date:
		cedex - FRANCE	WO200004149-A2,
			27 JAN. 2000
sbg1536324mapkkk	Mitogen-	SC: AL133380	Human mitogen-	Possibly
	activated	found at Sanger	activated protein kinase	cytosolic
	protein kinase	Centre	kinase kinase 10,
			gi: 4505263
			Eur. J. Biochem. 213
			(2), 701-710 (1993)
gsk1810944BrCaAg	Ankyrin	GB: AC060814	Human immunogenic	Possibly
	repeat/breast	Submitted (20 APR.	prostate tumour protein,	secreted
	cancer	2000) Whitehead	geneseqp: Y82017
	antigen	Institute/MIT Center	Filed by (CORI-)
		for Genome	CORIXA CORP
		Research, 320	Publication number and
		Charles Street,	filing date:
		Cambridge, MA	WO200004149-A2,
		02141, USA	27 JAN. 2000
sbg1535914AIP	Inhibitor of	GB: AC004223	Mouse putative protein,	Secreted
	apoptosis	Submitted (25 FEB.	gi: 12840594
	protein	1998) by Whitehead	Methods in
		Institute/MIT Center	enzymology. 303, 19-
		for Genome	44 (1999)
		Research, 320
		Charles Street,
		Cambridge, MA
		02141.
sbg1530894aCE	Carboxylesterase/	EMBL: AC007335	Macaca fascicularis	Secreted
	lipase	Submitted (03	hypothetical protein,
		NOV. 2000) by	gi: 13874532
		Production	Submitted (27 APR.
		Sequencing Facility,	2001) by Katsuyuki
		DOE Joint Genome	Hashimoto, National
		Institute, 2800	Institute of Infectious
		Mitchell Drive,	Diseases, Division of
		Walnut Creek, CA	Genetic Resources; 23-
		94598, USA	1, Toyama 1-chome,
			Shinjuku-ku, Tokyo
			162-8640, Japan
sbg1530894bCE	Carboxylesterase/	EMBL: AC007335	Macaca fascicularis	Cytosolic
	lipase	Submitted (03	hypothetical protein,
		NOV. 2000) by	gi: 13874532
		Production	Submitted (27 APR.
		Sequencing Facility,	2001) by Katsuyuki
		DOE Joint Genome	Hashimoto, National
		Institute, 2800	Institute of Infectious
		Mitchell Drive,	Diseases, Division of
		Walnut Creek, CA	Genetic Resources; 23-
		94598, USA	1, Toyama 1-chome,
			Shinjuku-ku, Tokyo
			162-8640, Japan
gsk.1813134.NaI	Sodium	GB: AC063951	Human solute carrier	Cytosolic
	iodide	Submitted (22 APR.	family 5 (sodium iodide
	symporter	2000) by Human	symporter),
		Genome Sequencing	gi: 4507035
		Center, Department	Biochem. Biophys. Res.
		of Molecular and	Commun. 226 (2), 339-
		Human Genetics,	345 (1996)
		Baylor College of
		Medicine, One
		Baylor Plaza,
		Houston, TX 77030,
		USA
gsk1811484BrCaAg	Ankyrin	GB: AC013269	Human immunogenic	Cytosolic
	repeat/breat	Submitted (05	prostate tumour protein,
	cancer	NOV. 1999)	geneseqp: Y82017
	antigen	Genome Sequencing	Filed by (CORI-)
		Center, Washington	CORIXA CORP
		University School of	Publication number and
		Medicine, 4444	filing date:
		Forest Park	WO200004149-A2,
		Parkway, St. Louis,	27 JAN. 2000
		MO 63108, USA
sbg1529984aSproteinase	Serine	GB: AC005570	Human plasminogen-	Secreted
	proteinase	Submitted (01 SEP.	like protein, geneseqp:
		1998) by Center for	AAE00290
		Human Genome	Filed by (HUMA-)
		Studies, DOE Joint	HUMAN GENOME
		Genome Institute,	SCI INC
		Los Alamos National	Publication number and
		Laboratory, MS	filing date:
		M888, Los Almos,	WO200124815-A1
		NM 87545, USA	12 APR. 2001
sbg1529984bSproteinase	Serine	GB: AC005570	Human plasminogen-	Secreted
	proteinase	Submitted (01 SEP.	like protein, geneseqp:
		1998) by Center for	AAE00290
		Human Genome	Filed by (HUMA-)
		Studies, DOE Joint	HUMAN GENOME
		Genome Institute,	SCI INC
		Los Alamos National	Publication number and
		Laboratory, MS	filing
		M888, Los Almos,	date: WO200124815-A1
		NM 87545, USA	12 APR. 2001
SBGKIN113	G protein-	GB: AC068693	Squirrel cone opsin	Cytosolic
	coupled	Submitted (06	kinase GRK7,
	receptor	MAY 2000) by	gi: 4001826
	kinase	Whitehead	Weiss E R, Raman D,
		Institute/MIT Center	Shirakawa S, Ducceschi
		for Genome	M H, Bertram P T,
		Research, 320	Wong F, Kraft T W,
		Charles Street,	Osawa S.
		Cambridge, MA	Mol Vis 1998 Dec.
		02141, USA	8; 4:27
gsk305961GDNa	Serpin	GB: AL137780	Human glia-derived	Secreted
		Sanger Centre,	nexin I alpha precursor,
		Hinxton,	gi: 87514
		Cambridgeshire,	Biochemistry. 26 (20),
		CB10 1SA, UK	6407-6410 (1987)
gsk2402719_2395124BIG2	Neural cell	AC066608,	Rat neural cell adhesion	Secreted
	adhesion	AC087084,	protein BIG-2
	molecule	AC087094 (Clones:	precursor, gi: 1016012
		RP11-785A7, RP11-	Yoshihara, Y.,
		198p17, 94m9)	Kawasaki, M.,
		Human Genomic	Tamada, A., Nagata, S.,
		Center, Institute of	Kagamiyama, H. and
		Genetics, Chinese	Mori. K. J. Neurobiol.
		Academy of	28 (1), 51-69 (1995)
		Sciences, Datun
		Road, Beijing,
		Beijing 100101,
		P.R. China
sbg18525LRR b	Leucine-rich	GB: AC016030	Macaca fascicularis	Membrane-
	repeat (LLR)	Direct submitted	hypothetical protein,	bound
		(19 NOV. 1999) by	gi: 13358942
		Whitehead	Submitted (24 FEB.
		Institute/MIT Center	2001) by Katsuyuki
		for Genome	Hashimoto, National
		Research, 320	Institute of Infectious
		Charles Street,	Diseases, Division of
		Cambridge, MA	Genetic Resources; 23-
		02141, USA	1, Toyama 1-chome,
			Shinjuku-ku, Tokyo
			162-8640, Japan
SBhACRP30c	Complement	GB: AC007016	Human complement-	secreted
	C1q/TNF	Submitted (08 May	c1q tumor necrosis
		1999) by Department	factor-related protein,
		of Genetics,	gi: 14734515
		Stanford Human	Submitted (20 DEC.
		Genome Center, 855	2000) by
		Miranda Avenue,	Bioinformatics,
		Palo, CA 94304	ZymoGenetics, Inc.,
			1201 Eastlake Ave.
			East, Seattle, WA
			98102, USA
sbg123493SLITc	Slit-like	SC: AL157714	Macaca fascicularis	Membrane-
	protein	Submitted (20 JAN.	hypothetical protein,	bound
		2001) by Sanger	gi: 15208206
		Centre, Hinxton,	Submitted (14 AUG.
		Cambridgeshire,	2001) by Katsuyuki
		CB10 1SA, UK.	Hashimoto, National
			Institute of Infectious
			Diseases, Division of
			Genetic Resources;
			23-1, Toyama 1-
			chome, Shinjuku-ku,
			Tokyo 162-8640,
			Japan
sbg102200MCTc	Monocarb	GB: AC015918	Mouse riken cDNA	Membrane-
	oxylate	Directly submitted	ORF, AK005699	bound
	cotransporter	(17 NOV. 1999)	The RIKEN Genome
	(MCT1)	by Whitehead	Exploration Research
		Institute/MIT	Group Phase II Team
		Center for Genome	and FANTOM
		Research, 320	Consortium.
		Charles Street,	Nature 409, 685-690
		Cambridge, MA	(2001)
		02141, USA.

[0120]

TABLE III
		Associated
Gene Name	Uses	Diseases
sbg1571549cystatin-re	An embodiment of the invention	Cancer and wound
	is the use of sbg1571549cystatin-re	healing disorders
	in bone resorption and in a variety
	of pathological conditions, including
	cancer progression and metastasis,
	stroke, inflammatory diseases, and
	infections. A close homologue of
	sbg1571549cystatin-re is a cystatin-
	related protein, known as testatin,
	a family of small secreted protease
	inhibitors. Cystatins and their target
	proteases are likely to play important
	roles in normal processes such as bone
	resorption (Lerner, U. H., Johansson,
	L., Ranjso, M., Rosenquist, J. B.,
	Reinholt, F. P. & Grubb, A. 1997. Acta
	Physiol. Scand. 161, 8192) and in a
	variety of pathological conditions,
	including cancer progression and meta-
	stasis, stroke, inflammatory diseases,
	and infections (Henskens, Y. M.,
	Veerman, E. C. & Nieuw Amerongen, A. V.
	1996. Biol. Chem. Hoppe-Seyler 377,
	7186).
sbg1558434SRCR	An embodiment of the invention is the	Atherosclerosis
	use of sbg1558434SRCR in atherogenesis.
	Close homologues of sbg1558434SRCR are
	scavenger receptor cysteine-rich
	domains. The scavenger receptors were
	detected in the macrophages of lipid-
	rich atherosclerotic lesions
	(Matsumoto A, Naito M, Itakura H,
	Ikemoto S, Asaoka H, Hayakawa I,
	Kanamori H, Aburatani H, Takaku F,
	Suzuki H, et al. Proc Natl Acad Sci.
	USA 1990 Dec.; 87(23):9133-7).
	Recently, Gough et al. also demonstrated
	that in human and rabbit atherosclerotic
	lesions, the scavenger receptor class A
	was mainly expressed by macrophage-
	derived foam cells, whereas only a low
	level of expression was found in the
	aortic endothelium (Gough, P. J., Greaves,
	D. R., Suzuki, H., Hakkinen, T., Hiltunen,
	M. O., Turunen, M., Ylä Herttuala,
	S., Kodama, T., Gordon, S. 1999.
	Arterioscler. Thromb. Vasc. Biol.
	19:461-471).
sbg1546354LRR	An embodiment of the invention is the	Wound healing,
	use of sbg1546354LRR in wound healing	Parkinson's
	and tissue repair and in neural develop-	disease, and
	ment and the adult nervous system dys-	Alzheimer's
	functions including Parkinson's disease	disease
	and Alzheimer's disease. Close homologues
	of sbg1546354LRR are leucine-rich repeat
	(LRR) proteins such as connectin, slit,
	chaoptin, and Toll. Mouse brain cDNAs
	which encode three new independent LRR
	proteins (neuronal leucine-rich repeat:
	NLRR-1, NLRR-2, NLRR-3) were reported
	(Taguchi A, Wanaka A, Mori T, Matsumoto
	K, Imai Y, Tagaki T, Tohyama M. 1996.
	Brain Res Mol Brain Res Jan.; 35(1-2):
	31-40, Taniguchi H, Tohyama M, Takagi
	T. 1996 Brain Res Mol Brain Res Feb.;
	36(1):45-52). Both NLRR-1 and NLRR-2
	mRNAs were expressed primarily in the
	central nervous system (CNS); NLRR-1
	mRNA was also detected in the non-
	neuronal tissues such as cartilage,
	while NLRR-2 mRNA expression was con-
	fined to the CNS at all developmental
	stages. NLRR-3 mRNA increased in
	layers 2-3 in the injured cerebral
	cortex (Ishii N, Wanaka A, Tohyama M.
	1996. Brain Res Mol Brain Res Aug.;
	40(1):148-52). These results suggest
	that NLRR-1 and NLRR-2 may play sig-
	nificant but distinct roles in neural
	development and the adult nervous
	system. While NLRR-3 may be an
	important component of the patho-
	physiological response to brain
	injury.
sbg1555869CABP	An embodiment of the invention is the	Cancer, infection,
	use of sbg1555869CABP in cell growth	autoimmune disorder,
	and differentiation, cell cycle	hematopoietic
	regulation and metabolic control. Close	disorder, wound
	homologues of sbg1555869CABP are S-100,	healing disorders,
	a member of the calcium and zinc-	inflammation,
	binding protein family. Calcium ions	Alzheimer's disease,
	act as a second messenger to interact	neoplastic diseases,
	with calcium binding proteins, and thus	nervous system
	control biological processes. Many	related disorders, and
	diseases, including Alzheimer's disease	cardiovascular
	and neoplastic diseases, are associated	disorders
	with abnormal calcium ion levels
	(Schafer B W, Heizmann C W. 1996. Trends
	Biochem Sci 21:134-40). Therefore,
	intracellular calcium ion levels and
	calcium ion signaling must be tightly
	regulated. For example, S100 A10, serves
	as a ligand of the tyrosine kinase
	substrate, annexin II (Kube E, Weber K,
	Gerke V. 1991. Gene 102:255-9).
	Evidence support S-100 involvement in
	cell growth and differentiation, cell
	cycle regulation and metabolic control
	(Lackmann M., Cornish C. J., Simpson
	R. J., Moritz R. L., Geczy C. L. 1992.
	J. Biol. Chem. 267:7499-7504).
sbg1518024NKR	An embodiment of the invention is the	Cancer, infection, and
	use of sbg1518024NKR in the recognition	autoimmune disorder
	of target cells by natural killer (herein
	“NK”) cells and subsequent cell lysis.
	Close homologues of sbg1518024NKR are a
	family of cell surface molecules, the
	receptors of NK cells. This lysis is a
	defense mechanism against pathogens and
	tumor cells that downregulate MHC
	expression to avoid T-cell immune response.
	Normal cells can escape lysis because
	their MHC molecules are recognized by
	NK-cell inhibitory receptors (Fan Q R,
	Mosyak L, Winter C C, Wagtmann N, Long
	E O, Wiley D C. 1997. Nature 389:96-100).
sbg1525809WNT8	An embodiment of the invention is the	Cancer, infection,
	use of sbg1525809WNT8 in the regulation	autoimmune disorder,
	of axis formation and hindbrain	hematopoietic
	patterning. Close homologues of	disorder, wound
	sbg1525809WNT8 include mouse and	healing disorders, and
	chicken WNT-8. WNT-8 is only expressed	inflammation
	during embryogenesis (Bouillet P,
	Oulad-Abdelghani M, Ward S J, Bronner
	S, Chambon P, Dolle P. 1996 Mech Dev
	58:141-52, Hume C R, Dodd J. 1993
	Development 119:1147-60).
sbg1519904cdk	An embodiment of the invention is the	Cancer
	use of sbg1519904cdk, an important
	component in cell cycle control, as a
	potential target for novel anticancer
	drug discovery. Close homologues of
	sbg1519904cdk are Cdk4 and its close
	relative Cdk6. In mammalian cells,
	there are two classes of Cdks that
	function at the G1/S phase transition
	(Ekholm S V, Reed S I, Curr Opin Cell
	Biol 2000 Dec.; 12(6):676-84).
	Cdk4 and Cdk6 are driven by three D-
	type cyclins: D1, D2 and D3. The
	primary target of these activities
	is pRb and related protiens (Grana X,
	Garriga J, Mayol X. Oncogene 1998,
	17:3365-3383). The interaction of the
	pRb-E2F complexes with cell-cycle
	control genes has been shown to be
	important for pRb-mediated cell-
	cycle arrest (Zhang, P., Wong, C.,
	Liu, D., Finegold, M., Harper, J.
	W. and Elledge, S. J., 1999. Genes
	Dev. 13, pp. 213-224.). The E2F
	target genes induced as a consequence
	of the disruption of pRb-E2F complexes
	include some of the enzymes involved
	in DNA metabolism, some protooncogenes,
	and some cell-cycle regulatory pro-
	teins (Dyson, N. 1998. Genes Dev. 12,
	pp. 2245-2262). Cdk4 is also
	reported mis-regulated in 60-70% of
	human cancers (Soni R, Muller L, Furet
	P, Schoepfer J, Stephan C, Zumstein-
	Mecker S, Fretz H, Chaudhuri B.
	Biochem Biophys Res Commun 2000 Sep.
	7; 275(3):877-84).
sbg1521449connexin	An embodiment of the invention is	Cardiovascular
	the use of sbg1521449connexin in	disease, osteogenesis,
	the propagation of the action	and wound healing
	potential from the conduction	disorders
	system to the working myocardium.
	Close homologues of
	sbg1521449connexin include connexin
	43 and connexin 45. Two members of
	the connexin family of gap junction
	proteins, connexin 43 (Cx43; Gj alpha
	1) and connexin 45 (Cx45; Gj alpha
	6) have been associated with osteo-
	genesis (Civitelli R, Beyer E C,
	Warlow P M, Robertson A J, Geist S
	T, Steinberg T H. 1993. J Clin
	Invest 91:1888-1896). Human
	connexin 45 was cloned and, like
	Cx43, is expressed throughout the
	heart. Expression of Cx45 in heart
	is regional and associated with
	the atrioventricular node, the
	bundle and peripheral ventricular
	conduction system where it is
	located at the borderzone between
	the purkinje-fibers and the working
	ventricular myocardium. In this
	position, Cx45 may play a signifi-
	cant role in the propagation of
	the action potential from the con-
	duction system to the working
	myocardium (Kanter H L, Saffitz
	J E, Beyer E C. J Mol Cell Cardiol
	1994 Jul.; 26(7):861-8. S. R.
	Coppen, E. Dupont, S. Rothery and
	N. J. Severs. Circ. Res. 82
	(1998), pp. 232243). Cx45-deficient
	mouse embryos exhibited striking
	abnormalities in vascular development
	and died between embryonic day (E)
	9.5 and 10.5 (Kruger O, Plum A, Kim
	J, Winterhager E, Maxeiner S,
	Hallas G, Kirchhoff S, Traub O,
	Lamers W H, Willecke K. Development
	2000 Oct.; 127(19):4179-93).
sbg1522659Siglec	An embodiment of the invention is	Cancer, infection,
	the use of sbg1522659Siglec to help	autoimmune disorder,
	prevent undesirable self-	hematopoietic
	reactivity and tissue damage and	disorder, wound
	permit effective killing of non-	healing disorders, and
	sialylated pathogens. Close homo-	inflammation diseases
	logues of sbg1522659Siglec are
	the five proteins Siglec-5 to
	Siglec-9. Despite their sequence
	similarity, all novel Siglecs
	characterized to date are expressed
	on distinct subsets of hemopoietic
	cells, such as neutrophils (Siglec-
	5), B-cells (Siglec-6), natural
	killer (NK) cells (Siglec7), eosino-
	phils (Siglec-8) and neutrophils and
	monocytes (Siglec-9). Each of these
	Siglecs also exhibits distinct carbo-
	hydrate binding properties (Zhang
	J Q, Nicoll G, Jones C, Crocker P R.
	J Biol Chem 2000 Jul. 21; 275(29):
	22121-6, Crocker, P. R., Clark,
	E. A., Filbin, M., Gordon, S., Jones,
	Y., Kehrl, J. H., Kelm, S., Le
	Douarin, N., Powell, L., Roder, J.,
	Schnaar, R. L., Sgroi, D. C.,
	Stamenkovic, K., Schauer, R.,
	Schachner, M., van den Berg, T. K.,
	van der Merwe, P. A., Watt, S. M.,
	and Varki, A. (1998) Glycobiology 8,
	v). CD22 (Siglec-6) was shown to
	downregulate the B cell receptor-
	mediated cell triggering. In
	addition, cross-linking of Siglec-7
	in human NK cells inhibited signal
	transduction of spontaneous NK cell-
	mediated cytotoxicity (Falco, M.,
	Biassoni, R., Bottino, C., Vitale,
	M., Sivori, S., Augugliaro, R.,
	Moretta, L., and Moretta, A. (1999)
	J. Exp. Med. 190, 793-802). It
	is possible that the immune receptor
	tyrosine-based inhibitory motifs
	(ITIM)-containing Siglecs mediate
	negative regulation of cellular
	activation events via sialic acid
	recognition.
sbg1528389ITI	An embodiment of the invention is	Cancer, pancreatitis,
	the use of sbg1528389ITI to	inflammation,
	modulate premature activation of	respiratory and wound
	pancreatic proteases and decrease	healing disorder
	the rate of mucus digestion,
	possibly by lowering the threshold
	for pancreatitis. Close homologues
	of sbg1528389ITI include a pan-
	creatic secretory trypsin inhibitor
	(PSTI). sbg1528389ITI has a function-
	al domain similiar to pancreatic
	trypsin inhibitor and/or neutrophil
	elastase inhibitors. Based on
	genetic-linkage studies, PSTI muta-
	tions are associated with
	pancreatitis and are disease modi-
	fying, possibly by lowering the
	threshold for pancreatitis from
	other genetic or environmental
	factors (Pfutzer R H, Barmada M M,
	Brunskill A P, Finch R, Hart P S,
	Neoptolemos J, Furey W F, Whitcomb
	D C. Gastroenterology 2000 Sep.;
	119(3):615-23). PSTI was also
	shownd to prevent premature acti-
	vation of pancreatic proteases and
	to decrease the rate of mucus
	digestion by luminal proteases within
	the stomach and colon. In addition,
	it was suggested that PSTI may be
	involved in both the early and late
	phases of the healing response follow-
	ing injury by increasing the proli-
	feration of a variety of cell lines
	and stimulating cell migration
	(Marchbank T, Freeman T C, Playford
	R J. Digestion 1998; 59(3):167-74).
	Finally, neutrophil elastase in-
	hibitors and other members of Kunitz-
	type protease inhibitor family are
	suspected of playing an important
	role in the extra-cellular matrix
	biology which is a key part of
	tumoral invasion and chronic broncho-
	pulmonary diseases (Cuvelier A, Muir
	J F, Martin J P, Sesboue R. Rev Mal
	Respir 2000 Apr.; 17(2):437-46).
sbg1526964ISLR	An embodiment of the invention is	Cancer, infection,
	the use of sbg1526964ISLR, a	autoimmune disorder,
	secreted protein, for the inhibition	hematopoietic
	of neoplastic cell growth and	disorder, wound
	regulation of the orderly assembly	healing disorders, and
	of extracellular matrices. Close	inflammation disease
	homologues of sbg1526964ISLR are
	decorin D and slit, members of
	the immunoglobulin superfamily
	containing leucine-rich repeat
	(ISLR) and human PRO221 antitumour
	protein. Decorin has been shown
	to mediate fundamental cellular
	processes, including regulation
	of the orderly assembly of extra-
	cellular matrices, corneal trans-
	parency, tensile strength of skin
	and tendon, viscoelasticity of
	blood vessels, and tumor cell
	proliferation (Kresse, H., Hausser,
	H., and Schönherr, E. (1993) Ex-
	perientia (Basel) 49, 403-416.
	Iozzo, R. V. (1998) Annu. Rev.
	Biochem. 67, 609-652. Iozzo,
	R. V. (1999) J. Biol. Chem. 274,
	18843-18846). Slit protein
	participates in the formation and
	maintenance of the nervous and
	endocrine systems by protein-
	protein interactions (Itoh A,
	Miyabayashi T, Ohno M, Sakano S.
	Brain Res Mol Brain Res 1998
	Nov. 20; 62(2):175-86).
sbg1539439TSPK	An embodiment of the invention is	Cancer, infection,
	the use of sbg1539439TSPK in	autoimmune disorder,
	spermiogenesis, and signaling	hematopoietic
	mechanisms including cell pro-	disorder, wound
	liferation and transformation.	healing disorders, and
	Close homologues of	inflammation disease
	sbg1539439TSPK include mouse
	spermiogenesis assosciated
	serine/threonine kinase and
	Ser/Thr kinase Akt/PKB,
	and Ser/Thr kinase CK2.
	Some protein kinases are
	related to carcinogenesis
	and tumor progression (Maemura
	M, Iino Y, Koibuchi Y, Yokoe T,
	and Morishita Y; 1999; Oncology
	57. Arnaud Besson, Stephen M.
	Robbins and V. Wee Yong. European
	Journal of Biochemistry 263 (3),
	605-611. Suppl 2:37-44). Ser/Thr
	kinase Akt/PKB has been implicated in
	antiapoptosis and neuronal survival
	(Kauffmann-Zeh A, Rodriguez-Viciana
	P, Ulrich E, Gilbert C, Coffer P,
	Downward J, Evan G. Nature 385,
	544-548 (1997). Kennedy S G,
	Wagner A J, Conzen S D, Jordan J,
	Bellacosa A, Tsichlis P N, Hay N
	Genes Dev. 11, 701-713 (1997).
	Ikeuchi T, Shimoke K, Kubo T,
	Yamada M, Hatanaka H, Hum Cell 1998
	Sep.; 11(3):125-40). Another Ser/Thr
	kinase CK2 was shown to be rapid
	modulated in the in nuclear matrix
	response to hormonal and growth
	factor signals and may serve as a
	factor in promoting the transition
	of inactive to active nucleosome
	(Ahmed K. Crit Rev Eukaryot Gene
	Expr 1999; 9(3-4):329-36).
	Another Ser/Thr kinase CK2 was
	shown to be rapid modulated in the
	in nuclear matrix response to hor-
	monal and growth factor signals and
	it may serve as a factor in pro-
	moting the transition of inactive
	to active nucleosome (Ahmed K. Crit
	Rev Eukaryot Gene Expr 1999;
	9(3-4):329-36).
sbg1571349lipase	An embodiment of the invention is	Cancer, infection,
	the use of sbg1571349lipase in	autoimmune disorder,
	modulating esterase-mediated	hematopoietic
	degradation. Close homologues of	disorder, wound
	sbg1571349lipase are rabbit entero-	healing disorders, and
	cyte brush border protein and	inflammation diseases
	Phospholipases A2. Rabbit entero-
	cyte brush border protein which
	was found to have esterase and
	phospholipase activity (Boll W,
	Schmid-Chanda T, Semenza G, Mantei
	N. J Biol Chem 1993 Jun. 15;
	268(17):12901-11). Esterase-
	mediated degradation during
	transepithelial transport might
	be a limiting factor for its oral
	absorption, targeting the pro-drug
	to a region of the intestine with
	lower esterase activity could lead
	to an increase in oral absorption
	of the pro-drug (Van Gelder J,
	Shafiee M, De Clercq E, Penninckx
	F, Van den Mooter G, Kinget R,
	Augustijns P. Int J Pharm 2000 Sep.
	15; 205(1-2):93-100). Phos-
	pholipases A2 has contributed to
	the production of proinflammatory
	lipid mediators, moreover, it
	may be involved in cell responses
	such as apoptosis or tumor cell
	metastatic potential (Chaminade B,
	Le Balle F, Fourcade O, Nauze M,
	Delagebeaudeuf C, Gassama-Diagne A,
	Simon M F, Fauvel J, Chap H.
	Lipids 1999; 34 Suppl: S49-55).
sbg1565539aCONNEXIN	An embodiment of the invention is	Cardiovascular
	the use of sbg1565539aCONNEXIN in	diseases, osteogenesis,
	the propagation of the action	and wound healing
	potential from the conduction system	disorders disease
	to the working myocardium. Close
	homologues of sbg1565539aCONNEXIN
	are connexin 43 and connexin 45.
	Two members of the connexin family
	of gap junction proteins, connexin
	43 (Cx43; Gj alpha 1) and connexin
	45 (Cx45; Gj alpha 6) have been
	associated with osteogenesis
	(Civitelli R, Beyer E C, Warlow P
	M, Robertson A J, Geist S T,
	Steinberg T H. 1993. J Clin Invest
	91:1888-1896). Human connexin 45
	was cloned and, like Cx43, is
	expressed throughout the heart.
	Expression of Cx45 in heart is
	regional and associated with the
	atrioventricular node, the bundle and
	peripheral ventricular conduction
	system where it is located at the
	borderzone between the purkinje-
	fibers and the working ventricular
	myocardium. In this position, Cx45
	may play a significant role in the
	propagation of the action potential
	from the conduction system to the
	working myocardium (Kanter H L, Saffitz
	J E, Beyer E C. J Mol Cell Cardiol
	1994 Jul.; 26(7):861-8. S. R.
	Coppen, E. Dupont, S. Rothery and N.
	J. Severs. Circ. Res. 82 (1998), pp.
	232243). Cx45-deficient mouse embryos
	exhibited striking abnormalities in
	vascular development and died between
	embryonic day (E) 9.5 and 10.5 (Kruger
	O, Plum A, Kirn J, Winterhager E,
	Maxeiner S, Hallas G, Kirchhoff S,
	Traub O, Lamers W H, Willecke K.
	Development 2000 Oct.; 127(19):4179-
	93).
sbg1565539bCONNEXIN	An embodiment of the invention is the	Cardiovascular
	use of sbg1565539bCONNEXIN in the	diseases, osteogenesis,
	propagation of the action potential	and wound healing
	from the conduction system to the	disorders disease
	working myocardium. Close homologues
	of sbg1565539bCONNEXIN are connexin
	43 and connexin 45. Two members of
	the connexin family of gap junction
	proteins, connexin 43 (Cx43; Gj alpha
	1) and connexin 45 (Cx45; Gj alpha 6)
	have been associated with osteo-
	genesis (Civitelli R, Beyer E C,
	Warlow P M, Robertson A J, Geist S T,
	Steinberg T H. 1993. J Clin Invest
	91:1888-1896). Human connexin 45
	was cloned and, like Cx43, is expressed
	throughout the heart. Expression of
	Cx45 in heart is regional and
	associated with the atrioventricular
	node, the bundle and peripheral
	ventricular conduction system where
	it is located at the borderzone
	between the purkinje-fibers and the
	working ventricular myocardium. In
	this position, Cx45 may play a
	significant role in the propagation
	of the action potential from the
	conduction system to the working
	myocardium (Kanter H L, Saffitz
	J E, Beyer E C. J Mol Cell Cardiol
	1994 Jul.; 26(7):861-8. S. R. Coppen,
	E. Dupont, S. Rothery and N. J.
	Severs. Circ. Res. 82 (1998), pp.
	232243). Cx45-deficient mouse
	embryos exhibited striking
	abnormalities in vascular develop-
	ment and died between embryonic
	day (E) 9.5 and 10.5 (Kruger O,
	Plum A, Kirn J, Winterhager E,
	Maxeiner S, Hallas G, Kirchhoff
	S, Traub O, Lamers W H, Willecke
	K. Development 2000 Oct.; 127(19):
	4179-93).
sbg1563294acadherin	An embodiment of the invention is	Cancer, infection,
	the use of sbg1563294acadherin in	autoimmune
	epithelial polarization, epithelial	disorder,
	sheet or tube fusion, cell	hematopoietic
	migration, cell sorting, and axonal	disorder, wound
	patterning. A close homologue of	healing disorders and
	sbg1563294acadherin is desmocollin,	inflammation
	a memeber of the cadherin super-	diseases
	family. Antisense expression of a
	desmocollin gene in MDCK cells has
	showed the alteration in desmosome
	plaque assembly (Roberts G A,
	Burdett I D, Pidsley S C, King I A,
	Magee A I, Buxton R S. Eur J Cell
	Biol 1998 Jul.; 76(3):192-203).
	Fat-like cadherins were involved
	in epithelial morphogenesis,
	proliferation control, and epithelial
	planar polarization (Tepass U. Curr
	Opin Cell Biol 1999 Oct.; 11(5):
	540-8, Takeshi Yagi and Takeichi.
	Genes & Dev 2000. 14(10) 1169-1180).
	Moreover, cadherin has been implicated
	in tumor progression in carcinomas
	and cutaneous melanoma (Johnson J P.
	Cancer Metastasis Rev 1999; 18(3):
	345-57).
sbg1563294bcadherin	An embodiment of the invention is	Cancer, infection,
	the use of sbg1563294bcadherin in	autoimmune
	epithelial polarization, epithelial	disorder,
	sheet or tube fusion, cell migration,	hematopoietic
	cell sorting, and axonal patterning.	disorder, wound
	A close homologue of	healing disorders and
	sbg1563294bcadherin is desmocollin,	inflammation
	a memeber of the cadherin super-	diseases
	family. Antisense expression of a
	desmocollin gene in MDCK cells has
	showed the alteration in desmosome
	plaque assembly (Roberts G A, Burdett
	I D, Pidsley S C, King I A, Magee A I,
	Buxton R S. Eur J Cell Biol 1998 Jul.;
	76(3):192-203). Fat-like cadherins
	were involved in epithelial morpho-
	genesis, proliferation control, and
	epithelial planar polarization (Tepass
	U. Curr Opin Cell Biol 1999 Oct.;
	11(5):540-8, Takeshi Yagi and
	Takeichi. Genes & Dev 2000. 14(10)
	1169-1180). Moreover, cadherin has
	been implicated in tumor progression
	in carcinomas and cutaneous melanoma
	(Johnson J P. Cancer Metastasis Rev
	1999; 18(3):345-57).
sbg1563509ECM	An embodiment of the invention is the	Cancer, infection,
	use of sbg1563509ECM to modulate the	autoimmune
	regulation of cell proliferation,	disorder,
	adhesion, migration, defective cell	hematopoietic
	migration, and chromosome instability.	disorder, wound
	Close homologues of sbg1563509ECM are	healing disorders,
	human fibulin-1, fibulin-2, chicken	tissue fragility,
	thrombospondin (TSP), mouse TSP-1,	abnormal escape
	and C. elegans hemicentin. The C-	reflex,
	terminal segment of human fibulin	mispositioning of
	contains eight consecutive calcium-	the vas deferens and
	binding EGF-like domains (Zhang R	uterus
	Z, Pan T C, Zhang Z Y, Mattei M G,	and inflammation
	Timpl R, Chu M L Genomics 1994 Jul.	diseases
	15; 22(2):425-30). Fibulins
	have been identified as basement
	membrane and microfibrillar pro-
	teins with a broad binding
	repertoire for other extracellular
	ligands (Giltay R, Timpl R, Kostka
	G. Matrix Biol 1999 Oct.; 18(5):
	469-80). It was suggested that
	the functional role of fibulin-1
	and fibulin-2 in bone marrow was
	related to binding to the major
	cell adhesion protein fibronectin
	(Gu Y C, Nilsson K, Eng H, Ekblom
	M. Br J Haematol 2000 May; 109(2):
	305-13). TSP contains VWFC
	domains and EGF-like domains at
	its N-terminal segment (Lawler J,
	Duquette M, Ferro P. J Biol Chem
	1991 May 5; 266(13):8039-43).
	TSP is a multifunction adhesive
	protein with the ability to bind
	proteoglycans, cell surface re-
	ceptors, other proteins, and
	calcium ions and is involved in the
	regulation of cell proliferation,
	adhesion and migration (Bornstein
	P. J Cell Biol 1995 Aug.; 130 (3):
	503-6). TSP, as a endogenous
	angiogenesis inhibitor, has been
	shown to inhibit proliferation and
	migration of vascular endothelial
	cells in vitro and inhibit neovas-
	cularization and tumor growth in
	vivo (Streit, M., Riccardi L.,
	Velasco, P., Brown, L. F.,
	Hawighorst, T., Bornstein, P. and
	Detmar, M. 1999. Proc. Natl Acad.
	Sci. USA, 96, 14888-14893). TSP-1
	was found to suppresses wound
	healing and granulation tissue
	formation in the skin of transgenic
	mice (Michael Streit, Paula
	Velasco, Lucia Riccardi, Lisa
	Spencer, Lawrence F. Brown,
	Lauren Janes, Bernhard Lange-
	Asschenfeldt, Kiichiro Yano,
	Thomas Hawighorst, Luisa
	Iruela-Arispe and Michael Detmar.
	the EMBO Journal, Vol. 19, No.
	13 pp. 3272-3282, 2000). Mutants
	of hemicentin, a conserved extra-
	cellular member of the immuno-
	globulin superfamily in
	Caenorhabditis elegans , are shown
	to cause a syndrome of tissue
	fragility, defective cell
	migration, chromosome insta-
	bility, abnormal escape reflex,
	mispositioning of the vas
	deferens, uterus, mitotic chromo-
	some loss, and multinucleate
	cells in the germline (Vogel
	B E, Hedgecock E M. Development
	2001 Mar.; 128 (6):883-894).
sbg1552159Serprotease	An embodiment of the invention is	Cancer, infection,
	the use of sbg1552159Serprotease	autoimmune
	in modulating the various	disorder,
	enzyme activities as discussed	hematopoietic
	herein. Close homologues of	disorder, wound
	sbg1552159Serprotease are rat	healing disorders,
	serine protease, and Xenopus	and inflammation
	laevis ovochymase and oviductin	diseases
	which are both serine protease.
	Serine proteases exist as pre-
	cursors that are activated by
	specific and limited proteo-
	lysis, allowing regulation of
	enzyme activity. Examples of
	this type of regulation include
	blood coagulation, fibrinolysis,
	complement activation, and
	trypsinogen activation by entero-
	peptidase in digestion. The
	precise control of these activa-
	tion processes is crucial for
	normal physiological enzymatic
	function; misregulation of these
	enzymes can lead to pathological
	conditions (Takeuchi T, Shuman
	M A, Craik C S. Proc Natl Acad
	Sci USA 1999 Sep. 28; 96(20):
	11054-61). Evidence supports
	a role for serine proteases, such
	as blood-derived proteases throm-
	bin, tissue plasminogen activator
	and plasmin by entering into brain
	tissue during cerebrovascular
	insult, and causing the sequence
	of events that can lead to glial
	scarring, edema, seizure and
	neuronal death (Gingrich M B,
	Traynelis S F. Trends Neurosci
	2000 Sep.; 23(9):399-407).
	Ovochymase, with chymotrypsin-
	like (Phe-X) substrate specificity,
	was translated as part of an
	unusual polyprotein proenzyme
	and released during egg activation.
	Lindsay L L, Yang J C, Hedrick J L.
	Proc Natl Acad Sci USA 1999 Sep.
	28; 96(20):11253-8). Oviductin,
	the Xenopus laevis oviductal pro-
	tease was observed to process egg
	envelope glycoprotein gp43 to a
	sperm-penetrable form, increases
	sperm binding to envelopes
	(Lindsay L L, Wieduwilt M J,
	Hedrick J L. Biol Reprod 1999
	Apr.; 60(4):989-95).
sbg1551159thymosinb4	An embodiment of the invention is	Cancer, infection,
	the use of sbg1551159thymosinb4	autoimmune
	in regulating rapid actin polymer-	disorder,
	ization/depolymerization processes	hematopoietic
	implicated in cell motility. A	disorder, wound
	close homologue of	healing disorders,
	sbg1551159thymosinb4 is thymosin	and inflammation
	b4. Thymosin b4 is a small actin-	diseases
	binding protein of 43 residues
	whose role consists of sequester-
	ing a large pool of unpolymerized
	actin in cells (Fechheimer, M. &
	Zigmond, S. H. J. Cell Biol. 1993.
	123, 1-5). In mammalian tissues,
	it forms a 1:1 complex with ATP-G-
	actin (Safer, D., Elzinga, M. &
	Nachmias, V. T. J. Biol. Chem.
	1991. 266, 4029-4032). In
	Xenopus laevis , thymosin beta 4
	mRNA was maternally present at a
	low level and the transcript
	became abundant after gastrula-
	tion (Yamamoto M, Shoda A,
	Minamino N, Matsuo H, Nishimatsu
	S, Ueno N, Murakami K. Biochem
	Biophys Res Commun 1992 Apr. 15;
	184(1):93-9). The oxidized
	thymosin beta4 (thymosin beta 4
	sulfoxide) generated by monocytes
	in the presence of glucocorti-
	coids was demonstrated to contain
	attenuated intracellular G-actin
	sequestering activity but greatly
	enhanced extracellular signal to
	inhibit an inflammatory response
	(Young J D, Lawrence A J, MacLean
	A G, Leung B P, McInnes I B, Canas
	B, Pappin D J, Stevenson R D. Nat
	Med 1999 Dec.; 5(12):1424-7).
	Further more, thymosin beta 4
	stimulated keratinocyte migration
	in the Boyden chamber assay and
	enhanced wound healing in a rat
	full thickness wound model
	(Malinda K M, Sidhu G S, Mani H,
	Banaudha K, Maheshwari R K,
	Goldstein A L, Kleinman H K. J
	Invest Dermatol 1999 Sep.;
	113(3):364-8).
SBh1686954.SOCS	An embodiment of the invention	Hyperproliferation,
	is the use of SBh1686954.SOCS	systemic
	in potential treatment for	autoimmune
	disease, injury, or abnormality	disease,
	involving cytokine mediated	hyperimmunity,
	cellular responsivenes, such as,	developmental
	hyperimmunity, immunosuppression,	abnormality
	allergies and hypertension. Close
	homologues of SBh1686954.SOCS are
	SOCS. SOCS can be used for
	naturally occuring antibodies in
	autoimmune diseases as well as
	helpful for diagnosis of cancer
	(W09820023-A1, submitted by Walter
	and Eliza institute of Medical
	Research).
sbg1548844slit	An embodiment of the invention	Alzheimer's disease,
	is the use of sbg1548844slit to	cancer,
	regulate the formation and	gastrointestinal
	maintenance of the nervous and	ulceration,
	endocrine systems by protein-	neuropathy, wound
	protein interactions. Close	healing disorders and
	homologues of sbg1548844slit	inflammation
	are human Slit-1, -2, and -3,	diseases
	a leucine-rich repeat super-
	family. In Drosophila embryo-
	genesis, the slit gene had been
	shown to play a critical role
	in CNS midline formation. Human
	Slit-1, -2, and -3 mRNAs were
	exclusively expressed in the
	brain, spinal cord, and thyroid,
	respectively (Itoh A, Miyabayashi
	T, Ohno M, Sakano S. Brain Res
	Mol Brain Res 1998 Nov. 20;
	62(2):175-86). Similiar
	leucine-rich protein GAC1
	was also found to be amplified
	and overexpressed in malignant
	gliomas (Almeida A, Zhu X X, Vogt
	N, Tyagi R, Muleris M, Dutrillaux
	A M, Dutrillaux B, Ross D, Malfoy
	B, Hanash S. Oncogene 1998 Jun.
	11; 16(23):2997-3002). Slit
	proteins were recently identified
	as important regulators of axon
	guidance and cell migration in
	Drosophila and vertebrates, they
	were simultaneously identified
	as negative regulators, repell-
	ing various axonal and cell
	migrations in both invertebrates
	and vertebrates, and as positive
	regulators, stimulating branching
	and extension of at least one
	class of axons in vertebrates
	(Brose K, Tessier-Lavigne M.
	Curr Opin Neurobiol 2000 Feb.;
	10(1):95-102).
sbg1545249GGT	An embodiment of the invention	Cancer, infection,
	is the use of sbg1545249GGTto	autoimmune
	regulate the gamma-glutamyl	disorder,
	cycle and the conjugation of	hematopoietic
	carcinogens and toxins. A close	disorder, wound
	homologue of sbg1545249GGT is	healing disorders,
	Gamma-glutamyltranspeptidase.	and inflammation
	Gamma-glutamyltranspeptidase
	(GGT) catalyzes the first step
	in the extracellular breakdown
	of Glutathione (GSH) into
	its constituent amino acids,
	which can then be transported
	into the cell and used to main-
	tain the level of intracellular
	GSH. GSH, the most abundant
	intracellular nonprotein thiol,
	is the major reductant of
	cellular thiol groups and parti-
	cipates in many important bio-
	logical processes, including
	the gamma-glutamyl cycle and
	the conjugation of carcinogens
	and toxins (Potdar P D, Andrews
	K L, Nettesheim P, Ostrowski L
	E. Am J Physiol 1997 Nov.; 273(5
	Pt 1):L1082-9). Mice deficient
	in GGT appear normal at birth
	but grow slowly and suffer pre-
	mature death, demonstrating the
	importance of GGT to normal
	homeostasis (Lieberman, M. W.,
	A. L. Wiseman, Z. -Z. Shi, B. Z.
	Carter, R. Barrios, C. -N. Ou,
	P. Chevez-Barrios, Y. Wang, G. M.
	Habib, J. C. Goodman, S. L.
	Huang, R. M. Lebovitz, and M. M.
	Matzuk. Proc. Natl. Acad. Sci.
	USA 93:7923-7926, 1996).
	Increased GGT activity is also
	widely used as a marker of
	preneoplastic lesions in the
	liver during chemical carcino-
	genesis (Hanigan, M. H., and H.
	C. Pitot. Carcinogenesis 6:
	165-172, 1985).
SBh1813899.Y82017	An embodiment of the invention	Hyperproliferation,
	is the use of SBh1813899.Y82017	Breast and Prostate
	to inhibit the development of	cancer
	prostate cancer. Close homologues
	of SBh1813899.Y82017 contain
	cytostatic activity and have
	been identified as potential
	vaccines and gene therapies
	(WO200004149-A2, Human immuno-
	genic prostate tumour protein
	sequence SEQ ID NO: 378
	HARLOCKER S L, MITCHAM J L,
	XU J, YUQIU J, DILLON D C:
	CORIXA CORP,).
sbg1536324mapkkk	An embodiment of the invention	Cancer, infection,
	is the use of sbg1536324mapkkk	autoimmune
	in mediating cell proliferation,	disorder,
	cell differentiation, and cell	hematopoietic
	survival by regulating signaling	disorder, wound
	pathways activated by receptor	healing disorders,
	protein tyrosine kinases. Close	and inflammation
	homologues of sbg1536324mapkkk	diseases
	are mitogen-activated protein
	(MAP) kinases. Examples include:
	Mitogen-activated protein kinase
	kinase kinase 10, MLK2, a mixed
	lineage kinase 2 (tyr and ser/thr
	specificity) with a Src homology
	3 (SH3) domain at the N-terminus
	followed by leucine zipper domains
	and a proline rich domain (Dorow
	D S, Devereux L, Tu G F, Price G,
	Nicholl J K, Sutherland G R,
	Simpson R J. Eur J Biochem 1995
	Dec. 1; 234(2):492-500). It has
	been reported that MLK2 can
	directly bind and mediate activa-
	tion of MKK7 and SEK1, which in
	turn induces JNK activation (Syu-
	ichi Hirai, Kumi Noda, Tetsuo
	Moriguchi, Eisuke Nishida, Akio
	Yamashita, Tetsuya Deyama, Keiko
	Fukuyama, and Shigeo Ohno. J Biol
	Chem, Vol. 273, Issue 13, 7406-
	7412, Mar. 27, 1998). The JNK
	pathway is required for the normal
	regulation of AP-1 transcriptional
	activity (Yang, D., Tournier, C.,
	Wysk, M., Lu, H. T., Xu, J.,
	Davis, R. J. & Flavell, R. A.
	1997. Proc. Natl. Acad. Sci. USA
	94, 3004-3009) and has been impli-
	cated in the stress-induced apop-
	tosis of neurons (Riesgo-Escovar,
	J. R., Jenni, M., Fritz, A. &
	Hafen, E. (1996) Genes Dev. 10,
	2759-2768), the malignant
	transformation of pre-B cells
	(Xia, Z., Dickens, M., Raingeaud,
	J., Davis, R. J. & Greenberg, M.
	E. (1995) Science 270, 1326-
	133137), and the expression of
	E-selectin by endothelial cells
	(Read, M. A., Whitely, M. Z.,
	Gupta, S., Pierce, J. W., Best,
	J., Davis, R. J. & Collins, T.
	(1997) J. Biol. Chem. 272, 2753-
	2761).
gsk1810944BrCaAg	An embodiment of the invention	Breast and prostate
	is the use of gsk1810944BrCaAg	cancer
	to inhibit the development of
	prostate cancer. Close homo-
	logues of gsk1810944BrCaAg
	contain cytostatic activity
	and have been identified as
	potential vaccines and gene
	therapies (WO200004149-A2,
	Human immunogenic prostate
	tumour protein sequence SEQ
	ID NO: 378 HARLOCKER S L,
	MITCHAM J L, XU J, YUQIU J,
	DILLON D C: CORIXA CORP,).
sbg1535914AIP	An embodiment of the inven-	Cancer, neurological
	tion is the use of	diseases,
	sbg1535914AIP in preventing	autoimmune
	and/or treating neuronal	disorders, viral
	loss in animal models having	infections, and
	head injury, stroke, spinal	hematological
	cord trauma, and Alzheimer's	diseases
	disease. Close homologues of
	sbg1535914AIP are mouse cas-
	pase-3 and -7. The central
	mechanisms of IAP apoptotic
	suppression appeared to be
	through direct caspase and
	pro-caspase inhibition (pri-
	marily caspase 3 and 7) and
	modulation of and by the
	transcription factor NF-
	kappaB. It has been demon-
	strated that X-linked
	inhibitor of apoptosis pro-
	tein (Xiap) was an important
	element in the control of
	ovarian tumor growth and
	might be a point of regula-
	tion for cisplatin in the
	induction of apoptosis (Li
	J, Feng Q, Kim J M,
	Schneiderman D, Liston P,
	Li M, Vanderhyden B, Faught
	W, Fung M F, Senterman M,
	Korneluk R G, Tsang B K.
	Endocrinology 2001 Jan. 1;
	142(1):370-380). The strong-
	est evidence for IAP involve-
	ment in cancer was observed
	in the IAP called survivin.
	Although not observed in
	adult differentiated tissue,
	survivin was present in most
	transformed cell lines and
	cancers tested to date. Sur-
	vivin was shown to inhibit
	caspase directly and apop-
	tosis in general. Moreover,
	survivin protein levels corre-
	lated inversely with 5 year
	survival rates in colorectal
	cancer. Recent data has also
	implicated survivin in cell
	cycle control. The IAPs have
	been shown to be induced by
	NF-kappaB or v-Rel in mul-
	tiple cell lines and con-
	versely, HIAP1 and HIAP2 have
	been shown to activate NF-
	kappaB possibly forming a
	positive feed-back loop
	(LaCasse E C, Baird S,
	Korneluk R G, MacKenzie A E.
	Oncogene 1998 Dec. 24;
	17(25):3247-59). Mice null
	for caspase-3 displayed con-
	siderable neuronal expansion
	usually resulting in death
	by the second week of life.
	Consistent with the observa-
	tion that apoptosis plays a
	central role in human neuro-
	degenerative disease, cas-
	pase-3 activation has recent-
	ly been observed in stroke,
	spinal cord trauma, head in-
	jury and Alzheimer's disease.
	In severe spinal muscular
	atrophy (SMA), the neuronal
	specific inhibitor of apop-
	tosis (IAP) family member
	known as NAIF was often
	dysfunctional due to missense
	and truncation mutations
	(Robertson G S, Crocker S J,
	Nicholson D W, Schulz J B.
	Brain Pathol 2000 Apr.;
	10(2):283-92). Taken to-
	gether, these findings
	suggest that anti-apop-
	totic strategies may some
	day have utility in the
	treatment of cancer and
	neurodegenerative diseases.
sbg1530894aCE	An embodiment of the inven-	Cancer, infection,
	tion is the use of	metabolic diseases,
	sbg1530894aCE in modulating	and sperm
	cancerous cells and alter-	disfunction
	ing sperm differentiation
	and maturation. Close
	homologues of sbg1530894aCE
	are mammalian carboxyles-
	terases. Carboxylesterase
	overexpression was observed
	in the male reproductive
	tract of different species.
	Based on a well-known role
	of carboxylesterases in de-
	toxification of environmental
	chemicals such as organophos-
	phate pesticides, it was pro-
	posed that various male
	genital tract carboxyles-
	terases was able to protect
	the male reproductive system
	against xenobiotic influences
	that could provoke its dys-
	function, thus altering sperm
	differentiation and maturation
	(Mikhailov A T, Torrado M.
	Reprod Fertil Dev 1999;
	11(3):133-45). Mammalian
	carboxylesterases represent a
	multigene family, the products
	of which are localized in the
	endoplasmic reticulum of many
	tissues. Multiple carboxyles-
	terases are major determinants
	of the pharmacokinetic behavior
	of most therapeutic agents con-
	taining ester or amide bonds.
	Toxic metabolites converted
	from systemically administered
	prodrugs by recombinant adeno-
	viruses expressing carboxyles-
	terase (CE) in the local tumor
	milieu has been shown to sup-
	press tumor growth and attain
	regional control in a single
	organ (Crystal R G. Cancer
	Chemother Pharmacol 1999;
	43 Suppl: S90-9). It was
	proven to be a useful strategy
	for achieving high local con-
	centrations of the toxic
	product while avoiding the
	systemic toxicity that limits
	the use of chemotherapy agents.
	Thus, altering the activity
	of CE class has important
	clinical implications.
sbg1530894bCE	An embodiment of the invention	Cancer, infection,
	is the us of sbg1530894bCE in	metabolic diseases, and sperm
	modulating cancerous cells and	disfunction
	altering sperm differentiation
	and maturation. Close homo-
	logues of sbg1530894bCE are
	mammalian carboxylesterases.
	Carboxylesterase overexpres-
	sion was observed in the male
	reproductive tract of differ-
	ent species. Based on a well-
	known role of carboxyles-
	terases in detoxification of
	environmental chemicals such
	as organophosphate pesticides,
	it was proposed that various
	male genital tract carboxyles-
	terases was able to protect
	the male reproductive system
	against xenobiotic influences
	that could provoke its dys-
	function, thus altering sperm
	differentiation and maturation
	(Mikhailov A T, Torrado M.
	Reprod Fertil Dev 1999; 11(3):
	133-45). Mammalian carboxyl-
	esterases represent a multigene
	family, the products of which
	are localized in the endoplas-
	mic reticulum of many tissues.
	Multiple carboxylesterases are
	major determinants of the
	pharmacokinetic behavior of
	most therapeutic agents con-
	taining ester or amide bonds.
	Toxic metabolites converted
	from systemically administered
	prodrugs by recombinant adeno-
	viruses expressing carboxyles-
	terase (CE) in the local tumor
	milieu has been shown to sup-
	press tumor growth and attain
	regional control in a single
	organ (Crystal R G. Cancer
	Chemother Pharmacol 1999;
	43 Suppl: S90-9). It was
	proven to be a useful strategy
	for achieving high local
	concentrations of the toxic
	product while avoiding the
	systemic toxicity that limits
	the use of chemotherapy
	agents. Thus, altering the
	activity of CE class has
	important clinical impli-
	cations.
gsk.1813134.NaI	An embodiment of the inven-	Breast cancer,
	tion is the use of	thyroid disorders,
	gsk.1813134.NaI in the diag-	e.g. hypo-
	nosis and treatment of	thyroidism, hyper-
	thyroid disorders, e.g.	thyroidis, immune/
	hypo-thyroidism and hyper-	inflammatory
	thyroidis. In addition,	disorders,
	gsk.1813134.NaI may be	hyperproliferation,
	useful in the prevention	and Prostate cancer
	and treatment of membrane
	transport disorders and
	immune/inflammatory dis-
	orders. Close homologues
	of gsk.1813134.NaI are
	known to be specifically
	expressed in breast cancer
	cells. (W09728175-A1, Dai,
	G., Levy, O., Carrasco,
	N.; University of Yeshiva
	Einstein College,
	W0200026245, Azimzai, Y.,
	Corley, N. C., Guegler, K.
	J., et. al. Incyte
	Pharmaceutical Inc.
gsk1811484BrCaAg	An embodiment of the inven-	Breast and prostate
	tion is the use of	cancer
	gsk1811484BrCaAg to inhibit
	the development of prostate
	cancer. Close homologues of
	gsk1811484BrCaAg contain
	cytostatic activity and
	have been identified as
	potential vaccines and gene
	therapies (WO200004149-A2,
	Human immunogenic prostate
	tumour protein sequence SEQ
	ID NO: 378 HARLOCKER S L,
	MITCHAM J L, XU J, YUQIU J,
	DILLON D C: CORIXA CORP,).
sbg1529984aSproteinase	An embodiment of the inven-	Cancer, infection,
	tion is the use of	autoimmune
	sbg1529984aSproteinase, a	disorder,
	proteinase, in mediating	hematopoietic
	cellular infiltration,	disorder, wound
	cytokine activation, tissue	healing disorders,
	damage, remodeling, and re-	inflammation, brain
	pair. Close homologues of	damages, colitis
	sbg1529984aSproteinase are	diseases
	testisin and serine pro-
	teases. Testisin, a human
	serine proteinase, was found
	abundantly expressed only in
	the testis, and was lost in
	testicular tumors. It was
	also aberrantly expressed in
	some tumor cell lines of
	non-testis origin (Hooper J
	D, Bowen N, Marshall H,
	Cullen L M, Sood R, Daniels
	R, Stuttgen M A, Normyle J
	F, Higgs D R, Kastner D L,
	Ogbourne S M, Pera M F,
	Jazwinska E C, Antalis T M.
	Biochim Biophys Acta 2000
	Jun. 21; 1492(1):63-71).
	Serine proteases were also
	suggested to be involved the
	sequence of events that lead
	to glial scarring, edema,
	seizure and neuronal death
	(Gingrich M B, Traynelis S F.
	Trends Neurosci 2000 Sep.;
	23(9):399-407). A clear ele-
	vation in the levels and
	activation of serine pro-
	tease was also found in
	colitis (Tarlton J F,
	Whiting C V, Tunmore D,
	Bregenholt S, Reimann J,
	Claesson M H, Bland P W.
	Am J Pathol 2000 Dec.;
	157(6):1927-35).
sbg1529984bSproteinase	An embodiment of the inven-	Cancer, infection,
	tion is the use of	autoimmune
	sbg1529984bSproteinase, a	disorder,
	proteinase, in mediating	hematopoietic
	cellular infiltration,	disorder, wound
	cytokine activation, tissue	healing disorders,
	damage, remodeling, and re-	inflammation, brain
	pair. Close homologues of	damages, colitis
	sbg1529984bSproteinase are	diseases
	testisin and serine pro-
	teases. Testisin, a human
	serine proteinase, was
	found abundantly expressed
	only in the testis, and was
	lost in testicular tumors.
	It was also aberrantly ex-
	pressed in some tumor cell
	lines of non-testis origin
	(Hooper J D, Bowen N,
	Marshall H, Cullen L M,
	Sood R, Daniels R, Stuttgen
	M A, Normyle J F, Higgs D R,
	Kastner D L, Ogbourne S M,
	Pera M F, Jazwinska E C,
	Antalis T M. Biochim Biophys
	Acta 2000 Jun. 21; 1492(1):
	63-71). Serine proteases
	were also suggested to be
	involved the sequence of
	events that lead to glial
	scarring, edema, seizure and
	neuronal death (Gingrich M B,
	Traynelis S F. Trends Neurosci
	2000 Sep.; 23(9):399-407). A
	clear elevation in the levels
	and activation of serine pro-
	tease was also found in
	colitis (Tarlton J F,
	Whiting C V, Tunmore D,
	Bregenholt S, Reimann J,
	Claesson M H, Bland P W.
	Am J Pathol 2000 Dec.;
	157(6):1927-35).
SBGKIN113	An embodiment of the inven-	Cone
	tion is the use of	phototransduction
	SBGKIN113 in regulating	deficiency
	vertebrate cone phototrans-
	duction. Close homologues
	of SBGKIN113 are mouse GRK1
	and squirrel GRK7. The G
	protein-coupled receptor
	kinases (GRKs) are critical
	enzymes in the desensitiza-
	tion of activated G protein-
	coupled receptors. Mice
	lacking GRK1 (rhodopsin kin-
	ase) was found recently to
	have profoundly slowed re-
	covery of cone-driven re-
	tinal responses suggesting
	a major role of a specific
	GRK in the inactivation of
	vertebrate cone phototrans-
	duction (Lyubarsky A L, Chen
	C, Simon M I, Pugh E N. J
	Neurosci 2000 Mar. 15; 20(6):
	2209-17). It was observed
	that null mutation in GRK1
	slowed recovery kinetics of
	rod and cone phototrans-
	duction in man (Cideciyan A
	V, Zhao X, Nielsen L, Khani
	S C, Jacobson S G, Palczewski
	K. Proc Natl Acad Sci USA
	1998 Jan. 6; 95(1):328-33).
	Squirrel GRK7 was cloned
	from retina and was shown to
	phosphorylate bovine
	rhodopsin in a light-
	dependent manner similar to
	bovine GRK1. The presence of
	this kinase in cones suggests
	that it could function in
	cone cells as a cone opsin
	kinase (Weiss E R, Raman D,
	Shirakawa S, Ducceschi M H,
	Bertram P T, Wong F, Kraft
	T W, Osawa S. Mol Vis 1998
	Dec. 8; 4:27).
gsk305961GDNa	An embodiment of the inven-	Cancer, infection,
	tion is the use of	autoimmune
	gsk305961GDNa, a member of	disorder,
	the serpin family, in	hematopoietic
	regulating neurite outgrowth.	disorder, wound
	gsk305961GDNa contains a	healing disorder,
	perfect representative of	inflammation,
	the serpin characteristic	neurological diseases
	pattern (pdoc00256). A close	(neuroinflammatory,
	homologue of gsk305961GDNa	neurodegenerative,
	is a glial derived nexin.	memory impairment,
	Serpins (SERine Proteinase	epilepsy, learning
	INhibitors) are specific	impairment, brain
	extracellular protease in-	trauma, stroke,
	hibitors with activity in	multiple sclerosis,
	the Cterminus. Glial derived	amyleotrophic lateral
	nexin (GDN, protease nexin I,	sclerosis,
	protease inhibitor 7), with	encephalitis,
	activity against thrombin,	Huntington's
	trypsin and urokinase, is	Disease, Down's
	known to promote neurite	Syndrome,
	outgrowth. (R W Scott, B L	Kallmann's disease),
	Bergman, A Bajpai, R T Hersh,	angiogenesis, cellular
	H Rodriguez, B N Jones, C	migration, ovulation
	Barreda, S Watts and J B	disorders
	Baker. Protease nexin.
	Properties and a modified
	purification procedure. J.
	Biol. Chem., Vol.
	260, Issue 11, 7029-7034,
	Jun., 1985; Monard, D.,
	NOday, E., Limat, A. &
	Solomon, F. Prog. Brain
	Res. 58, 359-364, 1983).
gsk2402719_2395124BIG2	An embodiment of the inven-	Cancer,
	tion is the use of	infection, autoimmune
	gsk2402719_2395124BIG2 a	disorder,
	member of the axon-	hematopoietic
	associated cell adhesion	disorder, wound
	molecule subfamily, in the	healing disorder,
	formation, maintenance and	inflammation,
	plasticity of functional	neurological diseases
	neuronal networks. A close	(neuroinflammatory,
	homologue of	neurodegenerative,
	gsk2402719_2395124BIG2 is a	memory impairment,
	rat subfamily member BIG2,	epilepsy, learning
	which is been shown to pro-	impairment, brain
	mote neurite outgrowth when	trauma, stroke,
	used as a substrate for	multiple sclerosis,
	neurons in vitro. (Yoshihara	amyleotrophic lateral
	Y, Kawasaki M, Tamada A,	sclerosis,
	Nagata S, Kagamiyama H, Mori	encephalitis,
	K. Overlapping and differen-	Huntington's
	tial expression of BIG-2,	Disease, Down's
	BIG-1, TAG-1, and F3: four	Syndrome,
	members of an axon-associ-	Kallmann's disease),
	ated cell adhesion molecule	angiogenesis, cellular
	subgroup of the immunoglobu-	migration, ovulation
	lin superfamily. J Neurobiol	disorders
	1995 Sep.; 28(1):51-69).
sbg18525LRRb	An embodiment of the inven-	Cancer, infection,
	tion is the use of	autoimmune
	sbg18525LRRb, a leucine-	disorder,
	rich repeat protein, in	hematopoietic
	regulating protein-protein	disorder, wound
	interactions, such as cell	healing disorder,
	adhesion or receptor-ligand	inflammation,
	binding. Close homologues	gastrointestinal
	of sbg18525LRRb are	ulceration, and
	connectin, slit, chaoptin,	diseases in spinal
	and toll. It is likely that	cord, thyroid gland,
	leucine-rich repeat (LRR)	heart, trachea,
	proteins such as connectin,	thymus, lymph
	slit, chaoptin, and toll	node, muscular
	have important roles in	system, and nervous
	neuronal development and	system
	the adult nervous system as
	cell adhesion molecules
	(Taguchi A, Wanaka A, Mori
	T, Matsumoto K, Imai Y,
	Tagaki T, Tohyama M, 1996,
	Brain Res Mol Brain Res;
	35:31-4). At least one LRR
	was shown to be specifi-
	cally expressed on B cells,
	suggesting its role in
	immunization (Miyake K,
	Yamashita Y, Ogata M,
	Sudo T, Kimoto M, 1995.
	J Immunol 154:3333-40).
	Some studies have shown
	that brain injury can
	cause overexepression
	of neuronal LRR, sug-
	gesting that neuronal
	LRR may be an important
	component of the patho-
	physiological response
	to brain injury (Ishii
	N, Wanaka A, Tohyama M,
	1996, Brain Res Mol
	Brain Res 40:148-52).
SBhACRP30c	An embodiment of the inven-	Cancer, obesity,
	tion is the use of	anorexia,
	SBhACRP30c in regulating	inflammation,
	inflammation, cell pro-	cardiovadcular
	liferation, cell death,	disease, growth
	immunity, and/or energy	abnormalities
	homeostatis. Close homo-
	logues of SBhACRP30c are
	ACRP30, chipmunk Hib27,
	C1q complement proteins,
	TNF, and other members
	of the TNF superfamily.
	ACRP30 (Adipocyte Comple-
	ment-Related Protein of 30
	kDa) is made exclusively
	in adipocytes, and its
	expression is dysregulated
	in various forms of obesity
	(Hu, E, Liang, P and
	Spiegelman, B M. J. Biol.
	Chem 271, 10697-10703,
	1996). ACRP30 secretion is
	acutely stimulated by in-
	sulin (Scherer, P E, Williams
	S., Fogliano, M., Baldini,
	G. and Lodish, J Biol. Chem.
	270, 26746-26749, 1995)
	and is repressed by chroni-
	cally elevated levels of
	insulin. A related molecule,
	the Hib27 protein from Si-
	berian chipmunks, seems also
	to be involved in energy
	homeostasis, as its expres-
	sion is specifically ex-
	tinguished during hiber-
	nation (Takamatsu, N.,
	Ohba, K., Kondo, J., Kondo,
	N., and Shiba, T. Mol. Cell
	Biol. 13 1516-1521, 1993).
	Recently, it has been shown
	that the three dimensional
	structure of ACRP30 is
	superimposible with that of
	the TNF's, suggesting that
	these proteins may have a
	similar function and mode
	of action (Shapiro, L and
	Scherer PE., Current Biology
	8, 335-338, 1997). TNF's
	are known to play a role in
	energy homeostasis, where
	they are implicated in
	cachexia, obesity and in
	insulin resistance
	(Hotamisligil G S., and
	Spiegelman B M. Diabetes
	(1994) 43, 1271-1278;
	Teoman Uysal K., Wiesbrock
	S M, Marina M W and
	Hotamisligil G S., Nature
	389, 610-614, 1997).
	Based on EST expression
	data, SBHACRP30a is pri-
	marily or exclusively
	expressed in heart.
sbg123493SLITc	An embodiment of the inven-	Diseases in spinal
	tion is the use of	cord, thyroid gland,
	sbg123493SLITc, a secreted	ovary, prostate,
	protein, in preventing and	renal gland, small
	treatment of diseases in	intestine, heart,
	spinal cord, thyroid gland,	trachea, thymus,
	ovary, prostate, renal	lymph node,
	gland, small intestine,	muscular system
	heart, trachea, thymus,	and colon, pineal
	lymph node, muscular system	tumors and
	and colon. Close homologues	alleviation of
	of sbg123493SLITc are rat	precocious puberty
	slit protein and pineal
	gland specific gene-1 pro-
	tein. This slit protein
	binds Robo rec ptors and
	has an evolutionarily con-
	served role in repulsive
	axon guidance. Pineal
	gland specific gene-1
	protein, is used in the
	treatment of pineal tumors
	and alleviation of pre-
	cocious puberty. (Brose,
	K., Bland, K. S., Wang, K.
	H., Arnott, D., Henzel, W.
	Goodman, C. S., Tessier-
	Lavigne, M. and Kidd, T.
	Slit proteins bind Robo
	receptors and have an
	evolutionarily conserved
	role in repulsive axon
	guidance Cell 96 (6),
	795-806 (1999);
	Application number
	AAW09405, publication
	date, 12 Dec. 1996, sub-
	mitted by Human Genome
	Science Inc)
sbg102200MCTc	An embodiment of the inven-	Cancer, infection,
	tion is the use of	autoimmune
	sbg102200MCTc in regulating	disorder,
	pyruvate uptake. Close homo-	hematopoietic
	logues of sbg102200MCTc are	disorder, wound
	Chinese hamster and human	healing disorders,
	MCT1. Mouse H+-mono-	and inflammation
	carboxylate cotransporter
	(MCT1) was cloned and se-
	quenced from Ehrlich
	Lettre tumour cells, the
	sequence of MCT1 is 93%
	and 87% identical to MCT1
	from Chinese hamster and
	human, respectively. N-
	glycanase-F treatment and
	an in vitro translation
	experiments demonstrated
	that glycosylation was not
	required for MCT1 function
	(Carpenter L, Poole R C,
	Halestrap A P. 1996. Biochim
	Biophys Acta Mar. 13;
	1279(2):157-63). Chick
	monocarboxylate transporter
	MCT3 cloned from retinal
	pigment epithelium (RPE)
	cDNA library was found only
	expressed in RPE cells. A
	rat thyroid epithelial cell
	line FRTL transfected with
	pCl-neo/MCT3 showed enhanced
	pyruvate uptake suggesting
	that MCT3 may regulate lac-
	tate levels in the inter-
	photoreceptor space (Yoon H,
	Fanelli A, Grollman E F,
	Philp N J. 1997. Biochem
	Biophys Res Commun May 8;
	234 (1):90-4). In human,
	MCT2 has been implicated as
	a primary pyruvate trans-
	porter. The mRNAs of MCT1
	and MCT2 were found co-
	expressed in various human
	cancer cell lines, including
	the hematopoietic lineages
	HL60, K562, MOLT-4,
	Burkitt's lymphoma Raji, and
	solid tumor cells such as
	SW480, A549, and G361. These
	findings suggested that
	human MCT1 and MCT2 may have
	distinct biological roles
	(Lin R Y, Vera J C, Chaganti
	R S, Golde D W. 1998. J Biol
	Chem Oct. 30; 273(44):28959-
	65).

[0121] Table IV. Quantitative, Tissue-Specific mRNA Expression Detected Using SybrMan

[0122] Quantitative, tissue-specific, mRNA expression patterns of the genes were measured using SYBR-Green Quantitative PCR (Applied Biosystems, Foster City, Calif.; see Schmittgen T. D. et al., Analytical Biochemistry 285:194-204, 2000) and human cDNAs prepared from various human tissues. Gene-specific PCR primers were designed using the first nucleic acid sequence listed in the Sequence List for each gene. The threshold cycle (Ct) is defined as the fractional cycle number at which the reporter fluorescence generated by cleavage of the probe reaches a threshold defined as 10 times the background. In cases sequence detection system software predicted more than one PCR product, Taqman was used for the specific PCR amplification as indicated under the specific genes.

[0123] In each gene's first subset table, two replicate measurements of gene of identification (GOI) mRNA were measured from various human tissues (column 3 and 4). The average GOI mRNA copies of the two replicates were made from each tissue RNA (column 5). The average amount of 18S rRNA from each tissue RNA was measured (column 6) and used for normalization. To make each tissue with the same amount of 50 ng of 18S rRNA, the normalization factor (column 7) was calculated by dividing 50 ng with the amount of 18S rRNA measured from each tissue (column 6). The mRNA copies per 50 ng of total RNA were obtained by multipling each GOI normalization factor and the average mRNA copies (column 8).

[0124] Fold changes shown in each gene's second subset table were only calculated for disease tissues which have a normal counterpart. There are blanks in the fold change column for all samples that do not have counterparts. In addition, the fold change calculations are the fold change in the disease sample as compared to the normal sample. Accordingly, there will not be a fold change calculation next to any of the normal samples. For patient matched cancer pairs (colon, lung, and breast), each tumor is compared to its specific normal counterpart. When patient-matched normal/disease pairs do not exist, each disease sample was compared back to the average of all the normal samples of that same tissue type. For example, normal brain from the same patient that provided Alzheimer's brain is not applicable. Three normal brain samples and 4 Alzheimer's brain samples are used in the fold change. Three normal samples were averaged, and each of the Alzheimer's samples was compared back to that average.

[0125] Abbreviations

[0126] ALZ Alzheimer's Disease

[0127] CT CLONTECH (1020 East Meadow Circle Palo Alto, Calif. 94303-4230, USA)

[0128] KC Sample prepared by GSK investigator

[0129] COPD chronic obstructive pulmonary disease

[0130] endo endothelial

[0131] VEGF vascular endothelial growth factor

[0132] bFGF basic fibroblast growth factor

[0133] BM bone marrow

[0134] osteo osteoblast

[0135] OA osteoarthritis

[0136] RA rheumatoid arthritis

[0137] PBL peripheral blood lymphocytes

[0138] PBMNC peripheral blood mononuclear cells

[0139] HIV human immunodeficiency virus

[0140] HSV Herpes simplex virus

[0141] HPV human papilloma virus

[0142] Gene Name sbg1571549cystatin-re

[0143] Moderate to low overall expression. Highest normal expression is seen in the whole brain, hypothalamus, liver, head of pancreas, small intestine, and uterus. This pattern of expression suggests that this gene may be involved in diabetes or other metabolic diseases. Highest disease expression is seen in the normal/tumor breast samples as well as in the RA and OA synovium samples. Upregulation in 2 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in 2 of 4 Alzheimer's brain samples suggests involvement in Alzheimer's disease. Downregulation in 3 of 3 COPD samples and in 2 of 4 asthmatic lung samples suggests a potential role for this gene in chronic obstructive pulmonary disorder and asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Downregulation in the HIV-infected PBL cells and in the HSV-infected MRC5 cells suggests a role for this gene in HIV and HSV.

							copies
		Mean	Mean				of mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18S	18 S	50 ng
sbg1571549-	(sample 1	(sample	(sample	GOI	rRNA	rRNA	total
cystatin-re	and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 40	0	0	0.00	3.06	16.34	0.00
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0.18	0.19	0.19	0.96	52.36	9.69
Zenbio
Adrenal Gland Clontech	38.08, 38.33	0.8	0.7	0.75	0.61	81.97	61.48
Whole Brain Clontech	30.32, 30.44	59.6	55.83	57.72	7.24	6.91	398.58
Fetal Brain Clontech	39.19, 38.01	0.43	0.84	0.64	0.48	103.95	66.01
Cerebellum Clontech	39.49, 38.62	0.37	0.59	0.48	2.17	23.04	11.06
Cervix	38.35, 40	0.69	0.22	0.46	2.42	20.66	9.40
Colon	37.24, 33.17	1.28	12.27	6.78	2.71	18.45	125.00
Endometrium	38.93, 37.89	0.5	0.89	0.70	0.73	68.21	47.41
Esophagus	38.16, 36.9	0.77	1.55	1.16	1.37	36.50	42.34
Heart Clontech	38.51, 36.41	0.63	2.03	1.33	1.32	37.88	50.38
Hypothalamus	36.58, 37.03	1.85	1.44	1.65	0.32	155.28	255.43
Ileum	38.36, 33.12	0.69	12.61	6.65	2.58	19.38	128.88
Jejunum	32.5, 39.56	17.8	0.35	9.08	6.60	7.58	68.75
Kidney	35.43, 36.28	3.49	2.18	2.84	2.12	23.58	66.86
Liver	38.57, 32.25	0.61	20.46	10.54	1.50	33.33	351.17
Fetal Liver Clontech	38.06, 37.94	0.81	0.87	0.84	10.40	4.81	4.04
Lung	37.01, 34.41	1.45	6.15	3.80	2.57	19.46	73.93
Mammary Gland	33.49, 34	10.24	7.74	8.99	13.00	3.85	34.58
Clontech
Myometrium	36.23, 38.44	2.24	0.66	1.45	2.34	21.37	30.98
Omentum	37.67, 37.81	1.01	0.93	0.97	3.94	12.69	12.31
Ovary	40, 34.28	0	6.63	3.32	4.34	11.52	38.19
Pancreas	36.22, 40	2.26	0	1.13	0.81	61.80	69.84
Head of Pancreas	32.37, 36.38	19.09	2.06	10.58	1.57	31.85	336.78
Parotid Gland	33.64, 39.31	9.46	0.41	4.94	5.48	9.12	45.03
Placenta Clontech	36.31, 33.17	2.14	12.29	7.22	5.26	9.51	68.58
Prostate	38.64, 38.17	0.59	0.76	0.68	3.00	16.67	11.25
Rectum	38.55, 38.15	0.62	0.77	0.70	1.23	40.65	28.25
Salivary Gland Clontech	40, 40	0.23	0	0.12	7.31	6.84	0.79
Skeletal Muscle	36.02, 39.17	2.52	0.44	1.48	1.26	39.68	58.73
Clontech
Skin	35.55, 35.44	3.28	3.49	3.39	1.21	41.32	139.88
Small Intestine Clontech	37.51, 33.94	1.1	8.01	4.56	0.98	51.07	232.64
Spleen	36.06, 38.75	2.47	0.55	1.51	4.92	10.16	15.35
Stomach	34.58, 33.81	5.61	8.59	7.10	2.73	18.32	130.04
Testis Clontech	37.39, 37.34	1.18	1.21	1.20	0.57	87.87	105.01
Thymus Clontech	33.94, 37.28	8.01	1.25	4.63	9.89	5.06	23.41
Thyroid	35.69, 37.08	3.02	1.4	2.21	2.77	18.05	39.89
Trachea Clontech	37.53, 38.01	1.09	0.83	0.96	9.71	5.15	4.94
Urinary Bladder	38.1, 37.26	0.79	1.26	1.03	5.47	9.14	9.37
Uterus	31.16, 34.07	37.51	7.42	22.47	5.34	9.36	210.35
genomic	24.9	1208.23
b-actin	26.07	630.69
1.00E+05	17.45	100000
1.00E+05	17.57	100000
1.00E+04	21.26	10000
1.00E+04	20.74	10000
1.00E+03	24.49	1000
1.00E+03	24.38	1000
1.00E+02	29.46	100
1.00E+02	28.37	100
1.00E+01	36.37	10
1.00E+01	32.51	10
1.00E+00	37.91	1
1.00E+00	37.27	1
NTC	35.86	−1
NTC	39.15	−1
				copies of
				mRNA		Fold
	Reg			detected /		Change
Sample	number		Mean	50 ng		in
sbg1571549-	(GSK		GOI	total		Disease
cystatin-re	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	32.53	18.72	37.44	colon normal
colon tumor GW98-166	21940	32.71	16.83	33.66	colon tumor	−1.11
colon normal GW98-178	22080	35.41	3.3	6.60	colon normal
colon tumor GW98-177	22060	33.45	10.79	21.58	colon tumor	3.27
colon normal GW98-561	23514	32.21	22.71	45.42	colon normal
colon tumor GW98-560	23513	35.14	3.89	7.78	colon tumor	−5.84
colon normal GW98-894	24691	31.7	30.93	61.86	colon normal
colon tumor GW98-893	24690	32.11	24.11	48.22	colon tumor	−1.28
lung normal GW98-3	20742	30.69	56.9	113.80	lung normal
lung tumor GW98-2	20741	34.24	6.67	13.34	lung tumor	−8.53
lung normal GW97-179	20677	33.59	9.89	19.78	lung normal
lung tumor GW97-178	20676	31.55	33.83	67.66	lung tumor	3.42
lung normal GW98-165	21922	31.6	32.73	65.46	lung normal
lung tumor GW98-164	21921	32.03	25.34	50.68	lung tumor	−1.29
lung normal GW98-282	22584	35.05	4.11	8.22	lung normal
lung tumor GW98-281	22583	32.79	16.06	32.12	lung tumor	3.91
breast normal GW00-392	28750	27.24	452.07	452.07	breast normal
breast tumor GW00-391	28746	28.37	229.53	459.06	breast tumor	1.02
breast normal GW00-413	28798	26.81	585.37	585.37	breast normal
breast tumor GW00-412	28797	25.15	1596.82	3193.64	breast tumor	5.46
breast normal GW00-	27592-95	35.56	3.02	3.02	breast normal
235:238
breast tumor GW00-	27588-91	29.85	93.86	93.86	breast tumor	31.08
231:234
breast normal GW98-621	23656	32.1	24.26	48.52	breast normal
breast tumor GW98-620	23655	35.55	3.04	6.08	breast tumor	−7.98
brain normal BB99-542	25507	35.27	3.61	7.22	brain normal
brain normal BB99-406	25509	33.99	7.79	15.58	brain normal
brain normal BB99-904	25546	34.34	6.31	12.62	brain normal
brain stage 5 ALZ BB99-	25502	33.03	13.84	27.68	brain stage 5 ALZ	2.34
874
brain stage 5 ALZ BB99-	25503	31.1	44.41	88.82	brain stage 5 ALZ	7.52
887
brain stage 5 ALZ BB99-	25504	32.56	18.44	36.88	brain stage 5 ALZ	3.12
862
brain stage 5 ALZ BB99-	25542	33.24	12.2	24.40	brain stage 5 ALZ	2.07
927
CT lung	normal	32.12	24.04	48.08	CT lung Nml
lung 26	normal	26.05	928.5		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	38.83	0.42	0.42	lung 24 COPD	−38.16
lung 28	COPD	40	0	0.00	lung 28 COPD	−16.03
lung 23	COPD	40	0	0.00	lung 23 COPD	−16.03
lung 25	normal	40	0	0.00	lung 25 Nml
asthmatic lung ODO3112	29321	40	0	0.00	asthmatic lung	−16.03
asthmatic lung ODO3433	29323	40	0.2	0.40	asthmatic lung	−40.07
asthmatic lung ODO3397	29322	33.96	7.93	15.86	asthmatic lung	−1.01
asthmatic lung ODO4928	29325	35.14	3.9	7.80	asthmatic lung	−2.05
endo cells	control	34.25	6.65	6.65	endo cells
endo VEGF		36.2	2.05	2.05	endo VEGF	−3.24
endo bFGF		36.19	2.06	2.06	endo bFGF	−3.23
heart Clontech	normal	38.63	0.48	0.96	heart
heart (T-1) ischemic	29417	36.32	1.91	3.82	heart (T-1) ischemic	3.98
heart (T-14) non-	29422	34.07	7.41	14.82	heart (T-14) non-	15.44
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	34.43	5.97	11.94	heart (T-3399) DCM	12.44
adenoid GW99-269	26162	34.29	6.47	12.94	adenoid
tonsil GW98-280	22582	37.3	1.06	2.12	tonsil
T cells PC00314	28453	36.43	1.79	3.58	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		35.28	3.58	7.16	monocyte
B cells PC00665	28455	38.29	0.58	1.16	B cells
dendritic cells	28441	34.09	7.31	14.62	dendritic cells
neutrophils	28440	35.65	2.86	2.86	neutrophils
eosinophils	28446	37.31	1.05	2.10	eosinophils
BM unstim		38.46	0.53	0.53	BM unstim
BM stim	treated	40	0.2	0.20	BM stim	−2.65
osteo dif	treated	37.81	0.78	0.78	osteo dif	0.78
osteo undif		40	0	0.00	osteo undif
chondrocytes		32.19	22.93	57.33	chondrocytes
OA Synovium IP 12/01	29462	36.01	2.3	2.30	OA Synovium
OA Synovium NP10/01	29461	32.01	25.56	51.12	OA Synovium
OA Synovium NP57/00	28464	30.61	59.45	118.90	OA Synovium
RA Synovium	28466	28.04	279.01	558.02	RA Synovium
NP03/01
RA Synovium	28467	30.26	73.55	147.10	RA Synovium
NP71/00
RA Synovium	28475	30.62	59.33	118.66	RA Synovium
NP45/00
OA bone (biobank)	29217	40	0	0.00	OA bone (biobank)
OA bone Sample 1	J. Emory	34.61	5.36	10.72	OA bone
OA bone Sample 2	J. Emory	35.81	2.6	5.20	OA bone
Cartilage (pool)	Normal	31.89	27.57	55.14	Nml Cartilage (pool)
Cartilage (pool)	OA	32.68	17.14	34.28	OA Cartilage (pool)	−1.61
PEL unifected	28441	35.33	3.47	6.94	PBL unifected
PBL HTV IIIB	28442	39.75	0.24	0.48	PBL HIV IIIB	−14.46
MRC5 uninfected	29158	29.55	112.51	225.02	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	33.51	10.36	20.72	MRC5 HSV strain F	−10.86
W12 cells	29179	32.16	23.43	46.86	W12 cells
Keratinocytes	29180	32.05	24.96	49.92	Keratinocytes
B-actin control		26.37	765.93
genomic		25.7	1146.09
1.00E+05		18.02	100000
1.00E+05		18.16	100000
1.00E+04		21.71	10000
1.00E+04		21.87	10000
1.00E+03		25.65	1000
1.00E+03		25.51	1000
1.00E+02		30.97	100
1.00E+02		30.1	100
1.00E+01		32.32	10
1.00E+01		38.11	10
1.00E+00		40	0
1.00E+00		34.22	1
NTC		38.19	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample
		Fold Change in Disease
		Population Relative to
	Disease tissues	Normal
	colon tumor	−1.11
	colon tumor	3.27
	colon tumor	−5.84
	colon tumor	−1.28
	lung tumor	−8.53
	lung tumor	3.42
	lung tumor	−1.29
	lung tumor	3.91
	breast tumor	1.02
	breast tumor	5.46
	breast tumor	31.08
	breast tumor	−7.98
	brain stage 5 ALZ	2.34
	brain stage 5 ALZ	7.52
	brain stage 5 ALZ	3.12
	brain stage 5 ALZ	2.07
	lung 24 COPD	−38.16
	lung 28 COPD	−16.03
	lung 23 COPD	−16.03
	asthmatic lung	−16.03
	asthmatic lung	−40.07
	asthmatic lung	−1.01
	asthmatic lung	−2.05
	endo VEGF	−3.24
	endo bFGF	−3.23
	heart (T-1) ischemic	3.98
	heart (T-14) non-obstructive DCM	15.44
	heart (T-3399) DCM	12.44
	BM stim	−2.65
	osteo dif	0.78
	OA Cartilage (pool)	−1.61
	PBL HTV IIIB	−14.46
	MRC5 HSV strain F	−10.86

[0144] Gene Name sbg1571549cystatin-re

[0145] Gene Name sbg1558434SRCR

[0146] Moderate to low overall expression. This gene is expressed fairly ubiquitously in all normal samples analyzed with the highest levels of expression seen in the whole brain, fetal brain, endometrium, and parotid gland. Highest disease expression is seen in the normal and Alzheimer's brain samples and in the RA and OA bone samples. Upregulation in 2 of 4 breast tumor samples implicates this gene in breast cancer. Downregulation in 1 of 3 COPD samples and in 1 of 4 asthmatic lung samples suggests a potential role for this gene in chronic obstructive pulmonary disorder and asthma. Downregulation in the HIV-infected PBL cells and in the HSV-infected MRC5 cells suggests a role for this gene in HIV and HSV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample 1	(sample	(sample	GOI	rRNA	18 S	total
sbg1558434SRCR	and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	38.36, 38.46	5.73	5.4	5.57	3.06	16.34	90.93
Adipocytes Zenbio
Subcutaneous Adipose	39.06, 39.58	3.66	2.63	3.15	0.96	52.36	164.66
Zenbio
Adrenal Gland	39.97, 38.01	1.44	7.19	4.32	0.61	81.97	353.69
Clontech
Whole Brain Clontech	29.24, 29.36	2025.47	1877.04	1951.26	7.24	6.91	13475.52
Fetal Brain Clontech	37.04, 38.12	13.44	6.69	10.07	0.48	103.95	1046.26
Cerebellum Clontech	35.93, 36.29	27.41	21.74	24.58	2.17	23.04	566.24
Cervix	38.03, 40	7.11	1.83	4.47	2.42	20.66	92.36
Colon	37.31, 38.11	11.27	6.73	9.00	2.71	18.45	166.05
Endometrium	36.72, 38.11	16.48	6.76	11.62	0.73	68.21	792.63
Esophagus	40, 38.98	1.7	3.87	2.79	1.37	36.50	101.64
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 39.1	0	3.56	1.78	0.32	155.28	276.40
Ileum	36.38, 34.9	20.49	53.26	36.88	2.58	19.38	714.63
Jejunum	35.3, 34.3	41.09	78.41	59.75	6.60	7.58	452.65
Kidney	37.19, 40	12.16	0	6.08	2.12	23.58	143.40
Liver	38.75, 36.46	4.47	19.51	11.99	1.50	33.33	399.67
Fetal Liver Clontech	33.75, 34.3	111.17	78.04	94.61	10.40	4.81	454.83
Lung	36.52, 36.62	18.74	17.61	18.18	2.57	19.46	353.60
Mammary Gland	33.65, 32.72	118.53	215.49	167.01	13.00	3.85	642.35
Clontech
Myometrium	36.02, 35.39	25.8	38.86	32.33	2.34	21.37	690.81
Omentum	36.03, 36.27	25.7	22.05	23.88	3.94	12.69	302.98
Ovary	35.07, 35.58	47.55	34.44	41.00	4.34	11.52	472.29
Pancreas	39.37, 40	3.01	0	1.51	0.81	61.80	93.02
Head of Pancreas	36.64, 36.61	17.38	17.7	17.54	1.57	31.85	558.60
Parotid Gland	32.39, 31.96	267.75	353.03	310.39	5.48	9.12	2832.03
Placenta Clontech	36.81, 36.33	15.55	21.26	18.41	5.26	9.51	174.95
Prostate	37.41, 40	10.56	0	5.28	3.00	16.67	88.00
Rectum	36.41, 37.24	20.1	11.83	15.97	1.23	40.65	648.98
Salivary Gland	35.89, 37.94	28.06	7.54	17.80	7.31	6.84	121.75
Clontech
Skeletal Muscle	38.79, 40	4.37	0	2.19	1.26	39.68	86.71
Clontech
Skin	37.85, 40	7.95	0	3.98	1.21	41.32	164.26
Small Intestine	40, 39.26	0	3.23	1.62	0.98	51.07	82.48
Clontech
Spleen	36.13, 37.51	24.07	9.94	17.01	4.92	10.16	172.82
Stomach	37.71, 37.79	8.75	8.27	8.51	2.73	18.32	155.86
Testis Clontech	38.78, 37.36	4.39	10.94	7.67	0.57	87.87	673.55
Thymus Clontech	35.3, 35.43	41.14	37.7	39.42	9.89	5.06	199.29
Thyroid	38.37, 37.54	5.7	9.75	7.73	2.77	18.05	139.44
Trachea Clontech	37.12, 36.12	12.77	24.18	18.48	9.71	5.15	95.13
Urinary Bladder	36.06, 36.07	25.22	25.12	25.17	5.47	9.14	230.07
Uterus	36.75, 36.59	16.22	17.9	17.06	5.34	9.36	159.74
genomic	30.97	665.14
b-actin	26.24	13919.08
1.00E+05	21.78	100000
1.00E+05	21.42	100000
1.00E+04	26.73	10000
1.00E+04	26.98	10000
1.00E+03	30.72	1000
1.00E+03	31.14	1000
1.00E+02	35.32	100
1.00E+02	37.01	100
1.00E+01	38.96	10
1.00E+01	38.41	10
1.00E+00	39.65	1
1.00E+00	37.39	1
NTC	40	−1
NTC	37.08	−1
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1558434SRCR	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	30.6	1522.06	3044.12	colon normal
colon tumor GW98-166	21940	28.65	4895.5	9791.00	colon tumor	3.22
colon normal GW98-178	22080	32.98	366.41	732.82	colon normal
colon tumor GW98-177	22060	33.13	335.61	671.22	colon tumor	−1.09
colon normal GW98-561	23514	32.96	370.41	740.82	colon normal
colon tumor GW98-560	23513	32.32	545.47	1090.94	colon tumor	1.47
colon normal GW98-894	24691	32.23	574.3	1148.60	colon normal
colon tumor GW98-893	24690	32.82	403.39	806.78	colon tumor	−1.42
lung normal GW98-3	20742	30	2180.92	4361.84	lung normal
lung tumor GW98-2	20741	29.78	2492.04	4984.08	lung tumor	1.14
lung normal GW97-179	20677	29.53	2888.4	5776.80	lung normal
lung tumor GW97-178	20676	30.84	1317.26	2634.52	lung tumor	−2.19
lung normal GW98-165	21922	30.08	2083.87	4167.74	lung normal
lung tumor GW98-164	21921	28.84	4367.14	8734.28	lung tumor	2.10
lung normal GW98-282	22584	30.99	1208.68	2417.36	lung normal
lung tumor GW98-281	22583	31.14	1104.34	2208.68	lung tumor	−1.09
breast normal GW00-392	28750	31.07	1150.42	1150.42	breast normal
breast tumor GW00-391	28746	31.19	1068.66	2137.32	breast tumor	1.86
breast normal GW00-413	28798	37.01	32.76	32.76	breast normal
breast tumor GW00-412	28797	33.02	357.67	715.34	breast tumor	21.84
breast normal GW00-	27592-95	35.62	75.44	75.44	breast normal
235:238
breast tumor GW00-	27588-91	31.32	989.78	989.78	breast tumor	13.12
231:234
breast normal GW98-621	23656	28.72	4703.97	9407.94	breast normal
breast tumor GW98-620	23655	28.41	5654.66	11309.32	breast tumor	1.20
brain normal BB99-542	25507	27.9	7676.77	15353.54	brain normal
brain normal BB99-406	25509	28.57	5145.99	10291.98	brain normal
brain normal BB99-904	25546	29.05	3866.67	7733.34	brain normal
brain stage 5 ALZ BB99-	25502	28.31	6015.38	12030.76	brain stage 5 ALZ	1.08
874
brain stage 5 ALZ BB99-	25503	26.48	17975.27	35950.54	brain stage 5 ALZ	3.23
887
brain stage 5 ALZ BB99-	25504	27.66	8852.07	17704.14	brain stage 5 ALZ	1.59
862
brain stage 5 ALZ BB99-	25542	28.89	4235.74	8471.48	brain stage 5 ALZ	−1.31
927
CT lung	normal	31.88	709.49	1418.98	CT lung Nml
lung 26	normal	24.63	54579.32		lung 26 Nml
lung 27	normal	35.73	70.39	70.39	lung 27 Nml
lung 24	COPD	36.36	48.37	48.37	lung 24 COPD	−12.03
lung 28	COPD	32.44	506.79	506.79	lung 28 COPD	−1.15
lung 23	COPD	33.62	249.93	249.93	lung 23 COPD	−2.33
lung 25	normal	33.58	255.95	255.95	lung 25 Nml
asthmatic lung ODO3112	29321	35.26	93.39	93.39	asthmatic lung	−6.23
asthmatic lung ODO3433	29323	32.79	409.82	819.64	asthmatic lung	1.41
asthmatic lung ODO3397	29322	33.36	291.41	582.82	asthmatic lung	1.00
asthmatic lung ODO4928	29325	32.07	633.54	1267.08	asthmatic lung	2.18
endo cells	control	34.24	172.28	172.28	endo cells
endo VEGF		35.49	81.66	81.66	endo VEGF	−2.11
endo bFGF		34.74	128.01	128.01	endo bFGF	−1.35
heart Clontech	normal	32.35	535.93	1071.86	heart
heart (T-1) ischemic	29417	31.25	1030.24	2060.48	heart (T-1)	1.92
					ischemic
heart (T-14) non-	29422	30.72	1417.06	2834.12	heart (T-14) non-	2.64
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	30.34	1778.61	3557.22	heart (T-3399)	3.32
					DCM
adenoid GW99-269	26162	30.66	1468.08	2936.16	adenoid
tonsil GW98-280	22582	30.26	1874.15	3748.30	tonsil
T cells PC00314	28453	38.46	13.71	27.42	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		39.15	9.12	18.24	monocyte
B cells PC00665	28455	37.38	26.22	52.44	B cells
dendritic cells	28441	33.71	237.32	474.64	dendritic cells
neutrophils	28440	37.27	28.07	28.07	neutrophils
eosinophils	28446	39.45	7.59	15.18	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	0.00
osteo dif	treated	31.2	1062.37	1062.37	osteo dif	2.11
osteo undif		32.45	503.88	503.88	osteo undif
chondrocytes		30.75	1397.9	3494.75	chondrocytes
OA Synovium IP12/01	29462	29.3	3319.59	3319.59	OA Synovium
OA Synovium NP10/01	29461	30.09	2067.27	4134.54	OA Synovium
OA Synovium NP57/00	28464	29.57	2832.91	5665.82	OA Synovium
RA Synovium	28466	29.37	3184.64	6369.28	RA Synovium
NP03/01
RA Synovium	28467	28.32	5987.67	11975.34	RA Synovium
NP71/00
RA Synovium	28475	28.28	6138.69	12277.38	RA Synovium
NP45/00
OA bone (biobank)	29217	32.72	428.55	428.55	OA bone
					(biobank)
OA bone Sample 1	J. Emory	30.28	1850.45	3700.90	OA bone
OA bone Sample 2	J. Emory	31.23	1047.56	2095.12	OA bone
Cartilage (pool)	Normal	28.39	5721.72	11443.44	Nml Cartilage
					(pool)
Cartilage (pool)	OA	29.25	3432.68	6865.36	OA Cartilage	−1.67
					(pool)
PBL unifected	28441	38.72	11.74	23.48	PBL unifected
PBL HIV IIIB	28442	40	0	0.00	PBL HIV IIIB	−23.48
MRC5 uninfected (100%)	29158	31.52	878.24	1756.48	MRC5
					uninfected
					(100%)
MRC5 HSV strain F	29178	37.92	19.03	38.06	MRC5 HSV	−46.15
					strain F
W12 cells	29179	36.14	55.05	110.10	W12 cells
Keratinocytes	29180	35.36	88.09	176.18	Keratinocytes
B-actin control		25.47	32999.55
genomic		31.99	663.96
1.00E+05		22	100000
1.00E+05		22.24	100000
1.00E+04		27.59	10000
1.00E+04		27.53	10000
1.00E+03		32.07	1000
1.00E+03		32.22	1000
1.00E+02		39.16	100
1.00E+02		36.16	100
1.00E+01		39.45	10
1.00E+01		37.64	10
1.00E+00		40	0
1.00E+00		39.82	1
NTC		36.91	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0147] Gene Name sbg1558434SRCR

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      3.22
colon tumor                      −1.09
colon tumor                      1.47
colon tumor                      −1.42
lung tumor                       1.14
lung tumor                       −2.19
lung tumor                       2.10
lung tumor                       −1.09
breast tumor                     1.86
breast tumor                     21.84
breast tumor                     13.12
breast tumor                     1.20
brain stage 5 ALZ                1.08
brain stage 5 ALZ                3.23
brain stage 5 ALZ                1.59
brain stage 5 ALZ                −1.31
lung 24 COPD                     −12.03
lung 28 COPD                     −1.15
lung 23 COPD                     −2.33
asthmatic lung                   −6.23
asthmatic lung                   1.41
asthmatic lung                   1.00
asthmatic lung                   2.18
endo VEGF                        −2.11
endo bFGF                        −1.35
heart (T-1) ischemic             1.92
heart (T-14) non-obstructive DCM 2.64
heart (T-3399) DCM               3.32
BM stim                          0.00
osteo dif                        2.11
OA Cartilage (pool)              −1.67
PBL HIV IIIB                     −23.48
MRC5 HSV strain F                −46.15

[0148] Gene Name sbg1546354LRR

[0149] This primer set was shown to amplify two products of approximately equal proportions by the dissociation curve program. Moderate to low overall expression. Highest normal expression is seen in the whole brain, endometrium, and uterus. Good levels of expression are seen in all of the samples representing the female reproductive system. Highest disease expression is seen in the normal and Alzheimer's brain samples. Downregulation in 2 of 4 lung tumors and upregulation in 3 of 4 breast tumors implicates this gene in cancers of the lung and breast. Downregulation in 1 of 4 Alzheimer's brain samples may suggest a role in Alzheimer's disease. Downregulation in 3 of 3 COPD samples and in 3 of 4 asthmatic lung samples suggests a potential role for this gene in chronic obstructive pulmonary disorder and asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulation in the OA cartilage sample suggests a possible role for this gene in osteoarthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample 1	(sample	(sample	GOI	rRNA	rRNA	total
sbg1546354LRR	and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 40	0	0	0.00	3.06	16.34	0.00
Adipocytes Zenbio
Subcutaneous	40, 40	0.45	0.39	0.42	0.96	52.36	21.99
Adipose Zenbio
Adrenal Gland	38.55, 39.05	1.15	0.88	1.02	0.61	81.97	83.20
Clontech
Whole Brain	22.45, 22.92	7165.24	5554.84	6360.04	7.24	6.91	43922.93
Clontech
Fetal Brain Clontech	40, 39.61	0.46	0.65	0.56	0.48	103.95	57.69
Cerebellum Clontech	40, 34.21	0	12.15	6.08	2.17	23.04	139.98
Cervix	40, 36.23	0	4.05	2.03	2.42	20.66	41.84
Colon	40, 40	0.39	0	0.20	2.71	18.45	3.60
Endometrium	34.32, 32.75	11.42	26.84	19.13	0.73	68.21	1304.91
Esophagus	37.29, 37.76	2.28	1.77	2.03	1.37	36.50	73.91
Heart Clontech	39.67, 38.45	0.63	1.21	0.92	1.32	37.88	34.85
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	37.74, 38.72	1.78	1.05	1.42	2.58	19.38	27.42
Jejunum	34.06, 34.2	13.18	12.16	12.67	6.60	7.58	95.98
Kidney	39.29, 40	0.77	0	0.39	2.12	23.58	9.08
Liver	34, 37.64	13.6	1.89	7.75	1.50	33.33	258.17
Fetal Liver Clontech	39.07, 36.21	0.87	4.1	2.49	10.40	4.81	11.95
Lung	39.45, 39.92	0.71	0.57	0.64	2.57	19.46	12.45
Mammary Gland	38.43, 35.9	1.23	4.85	3.04	13.00	3.85	11.69
Clontech
Myometrium	38.88, 34.92	0.96	8.24	4.60	2.34	21.37	98.29
Omentum	37.96, 38.78	1.58	1.02	1.30	3.94	12.69	16.50
Ovary	33.4, 32.97	18.8	23.7	21.25	4.34	11.52	244.82
Pancreas	40, 39.83	0	0.58	0.29	0.81	61.80	17.92
Head of Pancreas	40, 40	0.5	0	0.25	1.57	31.85	7.96
Parotid Gland	37.46, 33.84	2.08	14.78	8.43	5.48	9.12	76.92
Placenta Clontech	36.58, 33.33	3.35	19.54	11.45	5.26	9.51	108.79
Prostate	39.19, 40	0.81	0.58	0.70	3.00	16.67	11.58
Rectum	40, 37.44	0	2.11	1.06	1.23	40.65	42.89
Salivary Gland	38.17, 40	1.41	0.42	0.92	7.31	6.84	6.26
Clontech
Skeletal Muscle	37.17, 40	2.43	0	1.22	1.26	39.68	48.21
Clontech
Skin	39.11, 39.87	0.85	0.56	0.71	1.21	41.32	29.13
Small Intestine	39.14, 38.66	0.83	1.08	0.96	0.98	51.07	48.77
Clontech
Spleen	38.75, 35.13	1.03	7.35	4.19	4.92	10.16	42.58
Stomach	39.38, 39.17	0.73	0.82	0.78	2.73	18.32	14.19
Testis Clontech	40, 39.46	0	0.7	0.35	0.57	87.87	30.76
Thymus Clontech	31.76, 31.99	45.85	40.36	43.11	9.89	5.06	217.92
Thyroid	37.48, 33.8	2.06	15.17	8.62	2.77	18.05	155.51
Trachea Clontech	34.12, 34.14	12.73	12.58	12.66	9.71	5.15	65.16
Urinary Bladder	33.97, 38.52	13.82	1.17	7.50	5.47	9.14	68.51
Uterus	30.75, 31.24	79.28	60.67	69.98	5.34	9.36	655.20
genomic	25.24	1579.11
b-actin	26	1042.55
1.00E+05	18	100000
1.00E+05	18.21	100000
1.00E+04	21.29	10000
1.00E+04	21.33	10000
1.00E+03	25.09	1000
1.00E+03	24.96	1000
1.00E+02	32.46	100
1.00E+02	29.76	100
1.00E+01	33.68	10
1.00E+01	37.73	10
1.00E+00	38.01	1
1.00E+00	37.89	1
NTC	40	0
NTC	39.22	−1
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
sbg1546354LRR	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	32.92	25.83	51.66	colon normal
colon tumor GW98-166	21940	33.26	21.39	42.78	colon tumor	−1.21
colon normal GW98-178	22080	32.74	28.67	57.34	colon normal
colon tumor GW98-177	22060	35.12	7.61	15.22	colon tumor	−3.77
colon normal GW98-561	23514	32.71	29.09	58.18	colon normal
colon tumor GW98-560	23513	33.09	23.59	47.18	colon tumor	−1.23
colon normal GW98-894	24691	32.44	33.79	67.58	colon normal
colon tumor GW98-893	24690	32.14	40.07	80.14	colon tumor	1.19
lung normal GW98-3	20742	32.37	35.14	70.28	lung normal
lung tumor GW98-2	20741	33.74	16.37	32.74	lung tumor	−2.15
lung normal GW97-179	20677	30.45	102.87	205.74	lung normal
lung tumor GW97-178	20676	34.06	13.68	27.36	lung tumor	−7.52
lung normal GW98-165	21922	30.69	89.75	179.50	lung normal
lung tumor GW98-164	21921	28.52	300.37	600.74	lung tumor	3.35
lung normal GW98-282	22584	30.49	100.24	200.48	lung normal
lung tumor GW98-281	22583	34.72	9.48	18.96	lung tumor	−10.57
breast normal GW00-392	28750	31.86	46.72	46.72	breast normal
breast tumor GW00-391	28746	29.77	150.15	300.30	breast tumor	6.43
breast normal GW00-413	28798	39.07	0.84	0.84	breast normal
breast tumor GW00-412	28797	34.47	10.94	21.88	breast tumor	26.05
breast normal GW00-	27592-95	38.66	1.05	1.05	breast normal
235:238
breast tumor GW00-	27588-91	34.58	10.25	10.25	breast tumor	9.76
231:234
breast normal GW98-621	23656	34.45	11.01	22.02	breast normal
breast tumor GW98-620	23655	35.28	6.93	13.86	breast tumor	−1.59
brain normal BB99-542	25507	24.64	2625.08	5250.16	brain normal
brain normal BB99-406	25509	27.93	418.16	836.32	brain normal
brain normal BB99-904	25546	26.28	1048.78	2097.56	brain normal
brain stage 5 ALZ BB99-	25502	29.47	177.72	355.44	brain stage 5 ALZ	−7.68
874
brain stage 5 ALZ BB99-	25503	25.5	1620.02	3240.04	brain stage 5 ALZ	1.19
887
brain stage 5 ALZ BB99-	25504	25.96	1251.61	2503.22	brain stage 5 ALZ	−1.09
862
brain stage 5 ALZ BB99-	25542	25.83	1348.09	2696.18	brain stage 5 ALZ	−1.01
927
CT lung	normal	32.74	28.6	57.20	CT lung Nml
lung 26	normal	29.2	205.78		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	40	0	0.00	lung 24 COPD	−19.69
lung 28	COPD	40	0	0.00	lung 28 COPD	−19.69
lung 23	COPD	40	0	0.00	lung 23 COPD	−19.69
lung 25	normal	37.63	1.87	1.87	lung 25 Nml
asthmatic lung ODO3112	29321	37.11	2.5	2.50	asthmatic lung	−7.88
asthmatic lung ODO3433	29323	35.13	7.54	15.08	asthmatic lung	−1.31
asthmatic lung ODO3397	29322	36.76	3.03	6.06	asthmatic lung	−3.25
asthmatic lung ODO4928	29325	37.12	2.48	4.96	asthmatic lung	−3.97
endo cells	control	35.13	7.56	7.56	endo cells
endo VEGF		36.92	2.79	2.79	endo VEGF	−2.71
endo bFGF		34.52	10.6	10.60	endo bFGF	1.40
heart Clontech	normal	37.63	1.87	3.74	heart
heart (T-1) ischemic	29417	35.2	7.28	14.56	heart (T-1)	3.89
					ischemic
heart (T-14) non-	29422	33.92	14.83	29.66	heart (T-14) non-	7.93
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	33.91	14.89	29.78	heart (T-3399)	7.96
					DCM
adenoid GW99-269	26162	36.84	2.91	5.82	adenoid
tonsil GW98-280	22582	38.06	1.47	2.94	tonsil
T cells PC00314	28453	34.48	10.87	21.74	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		40	0	0.00	monocyte
B cells PC00665	28455	37.71	1.8	3.60	B cells
dendritic cells	28441	34.1	13.44	26.88	dendritic cells
neutrophils	28440	33.53	18.38	18.38	neutrophils
eosinophils	28446	36.39	3.75	7.50	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	37.26	2.31	2.31	BM stim	2.31
osteo dif	treated	39.27	0.75	0.75	osteo dif	0.75
osteo undif		40	0	0.00	osteo undif
chondrocytes		35.46	6.27	15.68	chondrocytes
OA Synovium IP12/01	29462	34.8	9.09	9.09	OA Synovium
OA Synovium NP10/01	29461	37.51	2	4.00	OA Synovium
OA Synovium NP57/00	28464	31.86	46.85	93.70	OA Synovium
RA Synovium	28466	32.26	37.32	74.64	RA Synovium
NP03/01
RA Synovium	28467	33.22	21.88	43.76	RA Synovium
NP71/00
RA Synovium	28475	31.52	56.4	112.80	RA Synovium
NP45/00
OA bone (biobank)	29217	40	0	0.00	OA bone (biobank)
OA bone Sample 1	J. Emory	39.56	0.64	1.28	OA bone
OA bone Sample 2	J. Emory	36.53	3.46	6.92	OA bone
Cartilage (pool)	Normal	38.31	1.28	2.56	Nml Cartilage
					(pool)
Cartilage (pool)	OA	33.77	16.11	32.22	OA Cartilage	12.59
					(pool)
PBL unifected	28441	34.27	12.18	24.36	PBL unifected
PBL HIV IIIB	28442	34.68	9.69	19.38	PBL HIV IIIB	−1.26
MRC5 uninfected (100%)	29158	35.57	5.9	11.80	MRC5 uninfected
					(100%)
MRC5 HSV strain F	29178	38.1	1.44	2.88	MRC5 HSV strain F	−4.10
W12 cells	29179	36.18	4.2	8.40	W12 cells
Keratinocytes	29180	32.92	25.89	51.78	Keratinocytes
B-actin control		26.2	1097.83
genomic		25.78	1386.79
1.00E+05		18.35	100000
1.00E+05		18.44	100000
1.00E+04		21.88	10000
1.00E+04		21.87	10000
1.00E+03		25.52	1000
1.00E+03		26.11	1000
1.00E+02		33.25	100
1.00E+02		29.64	100
1.00E+01		39.97	10
1.00E+01		34.19	10
1.00E+00		38.21	1
1.00E+00		39.11	1
NTC		38.2	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0150] Gene Name sbg1546354LRR

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.21
colon tumor                      −3.77
colon tumor                      −1.23
colon tumor                      1.19
lung tumor                       −2.15
lung tumor                       −7.52
lung tumor                       3.35
lung tumor                       −10.57
breast tumor                     6.43
breast tumor                     26.05
breast tumor                     9.76
breast tumor                     −1.59
brain stage 5 ALZ                −7.68
brain stage 5 ALZ                1.19
brain stage 5 ALZ                −1.09
brain stage 5 ALZ                −1.01
lung 24 COPD                     −19.69
lung 28 COPD                     −19.69
lung 23 COPD                     −19.69
asthmatic lung                   −7.88
asthmatic lung                   −1.31
asthmatic lung                   −3.25
asthmatic lung                   −3.97
endo VEGF                        −2.71
endo bFGF                        1.40
heart (T-1) ischemic             3.89
heart (T-14) non-obstructive DCM 7.93
heart (T-3399) DCM               7.96
BM stim                          2.31
osteo dif                        0.75
OA Cartilage (pool)              12.59
PBL HIV IIIB                     −1.26
MRC5 HSV strain F                −4.10

[0151] Gene Name sbg1555869CABP

[0152] Failed.

[0153] Gene Name sbg1518024NKR

[0154] Moderate to low overall expression. The highest normal expression is seen in the liver with lower levels of expression in the tissues specific to the female reproductive system including the uterus, cervix, endometrium, myometrium, and the ovary. The highest disease expression is seen in the HIV-infected and uninfected peripheral blood lymphocytes. Upregulation in 2 of 4 colon tumor samples implicates this gene in colon cancer. Downregulation in 2 of 4 lung tumor samples suggests a potential role in cancer of the lung. Upregulation in 1 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in the VEGF-treated endothelial cell line implicates a possible role for this gene in angiogenesis. Upregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV. Moderate to high expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in the PBMNCs, B cells, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1518024NKR	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 37.18	0	0.53	0.27	3.06	16.34	4.33
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 39.59	0	0.12	0.06	0.61	81.97	4.92
Whole Brain Clontech	34.25, 37.04	3.37	0.58	1.98	7.24	6.91	13.64
Fetal Brain Clontech	39.3, 40	0.14	0	0.07	0.48	103.95	7.28
Cerebellum Clontech	35, 40	2.1	0	1.05	2.17	23.04	24.19
Cervix	33.92, 35.2	4.13	1.84	2.99	2.42	20.66	61.67
Colon	40, 37.27	0	0.5	0.25	2.71	18.45	4.61
Endometrium	37.16, 34.98	0.54	2.12	1.33	0.73	68.21	90.72
Esophagus	39.19, 40	0.15	0	0.08	1.37	36.50	2.74
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 39.13	0	0.15	0.08	0.32	155.28	11.65
Ileum	40, 37.58	0	0.41	0.21	2.58	19.38	3.97
Jejunum	34.43, 33.67	3	4.84	3.92	6.60	7.58	29.70
Kidney	40, 37.82	0	0.35	0.18	2.12	23.58	4.13
Liver	32.48, 32.79	10.26	8.41	9.34	1.50	33.33	311.17
Fetal Liver Clontech	35.88, 40	1.2	0	0.60	10.40	4.81	2.88
Lung	34.3, 35.15	3.26	1.9	2.58	2.57	19.46	50.19
Mammary Gland	37.99, 35.57	0.32	1.46	0.89	13.00	3.85	3.42
Clontech
Myometrium	36.21, 35.15	0.98	1.9	1.44	2.34	21.37	30.77
Omentum	40, 34.97	0	2.13	1.07	3.94	12.69	13.52
Ovary	33.51, 34.04	5.37	3.84	4.61	4.34	11.52	53.05
Pancreas	36.13, 36.05	1.03	1.08	1.06	0.81	61.80	65.20
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	34.91, 39.48	2.22	0.12	1.17	5.48	9.12	10.68
Placenta Clontech	40, 35.62	0	1.42	0.71	5.26	9.51	6.75
Prostate	36.16, 37.79	1.01	0.36	0.69	3.00	16.67	11.42
Rectum	40, 38.6	0	0.22	0.11	1.23	40.65	4.47
Salivary Gland Clontech	37.14, 34.66	0.54	2.59	1.57	7.31	6.84	10.70
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	38.65, 34.71	0.21	2.51	1.36	1.21	41.32	56.20
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	36.41, 38.53	0.86	0.23	0.55	4.92	10.16	5.54
Stomach	36.03, 34.11	1.09	3.67	2.38	2.73	18.32	43.59
Testis Clontech	40, 40	0	0	0.00	0.57	87.87	0.00
Thymus Clontech	33.89, 35.15	4.2	1.9	3.05	9.89	5.06	15.42
Thyroid	36.33, 40	0.9	0	0.45	2.77	18.05	8.12
Trachea Clontech	38.43, 36.09	0.24	1.05	0.65	9.71	5.15	3.32
Urinary Bladder	40, 37.25	0	0.51	0.26	5.47	9.14	2.33
Uterus	31.93, 32.48	14.54	10.24	12.39	5.34	9.36	116.01
genomic	24.9	1225.76
b-actin	27.36	258.77
1.00E+05	18.17	100000
1.00E+05	18.21	100000
1.00E+04	21.27	10000
1.00E+04	21.19	10000
1.00E+03	24.74	1000
1.00E+03	24.69	1000
1.00E+02	29.57	100
1.00E+02	29.1	100
1.00E+01	32.13	10
1.00E+01	34.09	10
1.00E+00	40	0
1.00E+00	35.2	1
NTC	40	0
NTC	40	0
				copies
				of mRNA		Fold
	Reg			detected /		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
sbg1518024NKR	identifier )	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	30.98	26.90	53.80	colon normal
colon tumor GW98-166	21940	33.55	7.50	14.99	colon tumor	−3.59
colon normal GW98-178	22080	37.16	1.46	2.91	colon normal
colon tumor GW98-177	22060	35.29	3.33	6.67	colon tumor	2.29
colon normal GW98-561	23514	39.1	0.64	1.29	colon normal
colon tumor GW98-560	23513	33.49	7.71	15.43	colon tumor	11.98
colon normal GW98-894	24691	35.69	2.78	5.56	colon normal
colon tumor GW98-893	24690	32.33	13.58	27.15	colon tumor	4.88
lung normal GW98-3	20742	30.32	37.99	75.98	lung normal
lung tumor GW98-2	20741	34.37	5.09	10.18	lung tumor	−7.46
lung normal GW97-179	20677	32.59	11.94	23.88	lung normal
lung tumor GW97-178	20676	31.04	26.08	52.16	lung tumor	2.18
lung normal GW98-165	21922	29.1	73.39	146.78	lung normal
lung tumor GW98-164	21921	34.05	5.91	11.83	lung tumor	−12.41
lung normal GW98-282	22584	34.42	4.97	9.95	lung normal
lung tumor GW98-281	22583	32.16	14.77	29.55	lung tumor	2.97
breast normal GW00-392	28750	32.66	11.54	11.54	breast normal
breast tumor GW00-391	28746	38.04	1.00	2.00	breast tumor	−5.76
breast normal GW00-413	28798	35.03	3.75	3.75	breast normal
breast tumor GW00-412	28797	30.75	30.31	60.63	breast tumor	16.16
breast normal GW00-	27592-95	37.22	1.42	1.42	breast normal
235:238
breast tumor GW00-	27588-91	36.24	2.18	2.18	breast tumor	1.53
231:234
breast normal GW98-621	23656	34.34	5.16	10.32	breast normal
breast tumor GW98-620	23655	34.52	4.75	9.49	breast tumor	−1.09
brain normal BB99-542	25507	34.07	5.86	11.72	brain normal
brain normal BB99-406	25509	40	0.45	0.89	brain normal
brain normal BB99-904	25546	36.33	2.09	4.18	brain normal
brain stage 5 ALZ BB99-	25502	35.45	3.10	6.20	brain stage 5 ALZ	1.11
874
brain stage 5 ALZ BB99-	25503	35.07	3.68	7.37	brain stage 5 ALZ	1.32
887
brain stage 5 ALZ BB99-	25504	35.35	3.24	6.49	brain stage 5 ALZ	1.16
862
brain stage 5 ALZ BB99-	25542	39.36	0.58	1.16	brain stage 5 ALZ	−4.83
927
CT lung	normal	31.4	21.68	43.35	CT lung Nml
lung 26	normal	33.97	6.14		lung 26 Nml
lung 27	normal	33.88	6.41	6.41	lung 27 Nml
lung 24	COPD	35.94	2.49	2.49	lung 24 COPD	−7.20
lung 28	COPD	35.07	3.68	3.68	lung 28 COPD	−4.86
lung 23	COPD	38.79	0.73	0.73	lung 23 COPD	−24.46
lung 25	normal	34.92	3.95	3.95	lung 25 Nml
asthmatic lung ODO3112	29321	32.7	11.31	11.31	asthmatic lung	−1.58
asthmatic lung ODO3433	29323	31.02	26.35	52.70	asthmatic lung	2.94
asthmatic lung ODO3397	29322	32.05	15.61	31.21	asthmatic lung	1.74
asthmatic lung ODO4928	29325	33.14	9.13	18.26	asthmatic lung	1.02
endo cells	control	40	0.45	0.45	endo cells
endo VEGF		35.32	3.29	3.29	endo VEGF	7.35
endo bFGF		40	0.45	0.45	endo bFGF	1.00
heart Clontech	normal	33.28	8.53	17.07	heart
heart (T-1) ischemic	29417	34.16	5.62	11.23	heart (T-1)	−1.52
					ischemic
heart (T-14) non-	29422	33.01	9.72	19.45	heart (T-14) non-	1.14
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	34.08	5.83	11.66	heart (T-3399)	−1.46
					DCM
adenoid GW99-269	26162	40	0.45	0.89	adenoid
tonsil GW98-280	22582	33.34	8.29	16.58	tonsil
T cells PC00314	28453	34.18	5.56	11.13	T cells
PBMNC		33.86	6.47	6.47	PBMNC
monocyte		34.74	4.29	8.57	monocyte
B cells PC00665	28455	32.36	13.38	26.75	B cells
dendritic cells	28441	40	0.45	0.89	dendritic cells
neutrophils	28440	30.85	28.78	28.78	neutrophils
eosinophils	28446	39.24	0.61	1.22	eosinophils
BM unstim		32.98	9.87	9.87	BM unstim
BM stim	treated	30.62	32.44	32.44	BM stim	3.29
osteo dif	treated	40	0.45	0.45	osteo dif	1.00
osteo undif		40	0.45	0.45	osteo undif
chondrocytes		35.26	3.38	8.45	chondrocytes
OA Synovium IP12/01	29462	34.36	5.11	5.11	OA Synovium
OA Synovium NP10/01	29461	37.48	1.27	2.54	OA Synovium
OA Synovium NP57/00	28464	33.51	7.64	15.28	OA Synovium
RA Synovium	28466	33.5	7.68	15.36	RA Synovium
NP03/01
RA Synovium	28467	30.54	33.83	67.67	RA Synovium
NP71/00
RA Synovium __—	28475	34.1	5.78	11.55	RA Synovium
NP45/00
OA bone (biobank)	29217	32.39	13.18	13.18	OA bone (biobank)
OA bone Sample 1	J. Emory	34.31	5.23	10.47	OA bone
OA bone Sample 2	J. Emory	33.15	9.09	18.17	OA bone
Cartilage (pool)	Normal	33.56	7.46	14.92	Nml Cartilage
					(pool)
Cartilage (pool)	OA	34.94	3.91	7.82	OA Cartilage	−1.91
					(pool)
PBL unifected	28441	27.6	171.44	342.88	PBL unifected
PBL HIV IIIB	28442	26.15	407.26	814.52	PBL HIV IIIB	2.38
MRC5 uninfected	29158	40	0.45	0.89	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	35.02	3.77	7.54	MRC5 HSV strain F	8.43
W12 cells	29179	40	0.45	0.89	W12 cells	1.00
Keratinocytes	29180	40	0.45	0.89	Keratinocytes
B-actin control		27.29	205.48
genomic		24.73	996.82		Disease plate did not have
					genomic samples.
1.00E+05		40	0.45		Copy number was, therefore
					calculated from the gene-
					specific genomic curve on
					the normal plate.
1.00E+05		40	0.45
1.00E+04		39.72	0.50
1.00E+04		40	0.45
1.00E+03		40	0.45
1.00E+03		40	0.45
1.00E+02		38.14	0.96
1.00E+02		40	0.45
1.00E+01		40	0.45
1.00E+01		40	0.45
1.00E+00		40	0.45
1.00E+00		38.5	0.83
NTC		40	0.45
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0155] Gene Name sbg1518024NKR

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −3.59
colon tumor                      2.29
colon tumor                      11.98
colon tumor                      4.88
lung tumor                       −7.46
lung tumor                       2.18
lung tumor                       −12.41
lung tumor                       2.97
breast tumor                     −5.76
breast tumor                     16.16
breast tumor                     1.53
breast tumor                     −1.09
brain stage 5 ALZ                1.11
brain stage 5 ALZ                1.32
brain stage 5 ALZ                1.16
brain stage 5 ALZ                −4.83
lung 24 COPD                     −7.20
lung 28 COPD                     −4.86
lung 23 COPD                     −24.46
asthmatic lung                   −1.58
asthmatic lung                   2.94
asthmatic lung                   1.74
asthmatic lung                   1.02
endo VEGF                        7.35
endo bFGF                        1.00
heart (T-1) ischemic             −1.52
heart (T-14) non-obstructive DCM 1.14
heart (T-3399) DCM               −1.46
BM stim                          3.29
osteo dif                        1.00
OA Cartilage (pool)              −1.91
PBL HIV IIIB                     2.38
MRC5 HSV strain F                8.43
W12 cells                        1.00

[0156] Gene Name sbg1525809WNT8

[0157] Low overall expression. The highest normal expression is seen in the whole brain, testis, liver, parotid gland, and thymus. Some of the tissues representing the GI tract including the colon and the jejunum are represented as well as some of the tissue of the female reproductive system including the endometrium, ovary, and uterus. The highest disease expression is seen in the normal and disease brain samples. Upregulation in 1 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1525809WNT8	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 40	0	0	0.00	3.06	16.34	0.00
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	29.74, 29.32	643.92	815.05	729.49	7.24	6.91	5037.88
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	40, 40	0	0	0.00	2.17	23.04	0.00
Cervix	40, 40	0	0	0.00	2.42	20.66	0.00
Colon	40, 37.05	0	10.82	5.41	2.71	18.45	99.82
Endometrium	37.21, 40	9.89	0	4.95	0.73	68.21	337.31
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	40, 40	0	0	0.00	2.58	19.38	0.00
Jejunum	33.11, 36.95	98.1	11.44	54.77	6.60	7.58	414.92
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	35.45, 40	26.55	0	13.28	1.50	33.33	442.50
Fetal Liver Clontech	40, 40	0	0	0.00	10.40	4.81	0.00
Lung	40, 40	0	0	0.00	2.57	19.46	0.00
Mammary Gland	40, 40	0	0	0.00	13.00	3.85	0.00
Clontech
Myometrium	40, 40	0	0	0.00	2.34	21.37	0.00
Omentum	40, 40	0	0	0.00	3.94	12.69	0.00
Ovary	35.51, 36.3	25.61	16.49	21.05	4.34	11.52	242.51
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	36.18, 36.67	17.64	13.4	15.52	5.48	9.12	141.61
Placenta Clontech	40, 40	0	0	0.00	5.26	9.51	0.00
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland Clontech	40, 40	0	0	0.00	7.31	6.84	0.00
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	40, 40	0	0	0.00	4.92	10.16	0.00
Stomach	40, 40	0	0	0.00	2.73	18.32	0.00
Testis Clontech	40, 37.35	0	9.13	4.57	0.57	87.87	401.14
Thymus Clontech	37.64, 35.26	7.78	29.5	18.64	9.89	5.06	94.24
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	37.13, 40	10.38	0	5.19	9.71	5.15	26.73
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	36.99, 35.76	11.22	22.23	16.73	5.34	9.36	156.60
genomic	28.79	1094.71
b-actin	27.79	1919.17
1.00E+05	20.91	100000
1.00E+05	21.03	100000
1.00E+04	24.51	10000
1.00E+04	24.59	10000
1.00E+03	28.47	1000
1.00E+03	28.76	1000
1.00E+02	34.37	100
1.00E+02	32.84	100
1.00E+01	37.11	10
1.00E+01	36.96	10
1.00E+00	40	0
1.00E+00	40	1
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1525809WNT8	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	36.83	7.92	15.84	colon normal
colon tumor GW98-166	21940	37.72	5.11	10.23	colon tumor	−1.55
colon normal GW98-178	22080	40	1.75	3.49	colon normal
colon tumor GW98-177	22060	37.27	6.37	12.74	colon tumor	3.65
colon normal GW98-561	23514	36.48	9.43	18.87	colon normal
colon tumor GW98-560	23513	39.67	2.03	4.06	colon tumor	−4.64
colon normal GW98-894	24691	36.16	11.09	22.17	colon normal
colon tumor GW98-893	24690	39.74	1.97	3.93	colon tumor	−5.64
lung normal GW98-3	20742	39.11	2.64	5.27	lung normal
lung tumor GW98-2	20741	40	1.75	3.49	lung tumor	−1.51
lung normal GW97-179	20677	37.48	5.75	11.50	lung normal
lung tumor GW97-178	20676	35.75	13.66	27.32	lung tumor	2.38
lung normal GW98-165	21922	36.45	9.58	19.15	lung normal
lung tumor GW98-164	21921	35.43	16.11	32.21	lung tumor	1.68
lung normal GW98-282	22584	37.39	6.01	12.01	lung normal
lung tumor GW98-281	22583	40	1.75	3.49	lung tumor	−3.44
breast normal GW00-392	28750	40	1.75	1.75	breast normal
breast tumor GW00-391	28746	33.26	51.26	102.52	breast tumor	58.73
breast normal GW00-413	28798	37.05	7.10	7.10	breast normal
breast tumor GW00-412	28797	36.5	9.34	18.68	breast tumor	2.63
breast normal GW00-	27592-95	37.45	5.83	5.83	breast normal
235:238
breast tumor GW00-	27588-91	35.98	12.15	12.15	breast tumor	2.08
231:234
breast normal GW98-621	23656	35.2	18.15	36.29	breast normal
breast tumor GW98-620	23655	39.59	2.11	4.22	breast tumor	−8.61
brain normal BB99-542	25507	30.39	267.68	535.36	brain normal
brain normal BB99-406	25509	30.94	192.72	385.45	brain normal
brain normal BB99-904	25546	32.17	94.37	188.74	brain normal
brain stage 5 ALZ BB99-	25502	30.61	234.55	469.10	brain stage 5 ALZ	1.27
874
brain stage 5 ALZ BB99-	25503	30.11	317.14	634.27	brain stage 5 ALZ	1.71
887
brain stage 5 ALZ BB99-	25504	30.57	240.24	480.47	brain stage 5 ALZ	1.30
862
brain stage 5 ALZ BB99-	25542	29.5	461.38	922.75	brain stage 5 ALZ	2.49
927
CT lung	normal	40	1.75	3.49	CT lung Nml
lung 26	normal	33.69	40.51		lung 26 Nml
lung 27	normal	40	1.75	1.75	lung 27 Nml
lung 24	COPD	40	1.75	1.75	lung 24 COPD	−1.33
lung 28	COPD	40	1.75	1.75	lung 28 COPD	−1.33
lung 23	COPD	40	1.75	1.75	lung 23 COPD	−1.33
lung 25	normal	40	1.75	1.75	lung 25 Nml
asthmatic lung ODO3112	29321	38.56	3.42	3.42	asthmatic lung	1.47
asthmatic lung ODO3433	29323	40	1.75	3.49	asthmatic lung	1.50
asthmatic lung ODO3397	29322	40	1.75	3.49	asthmatic lung	1.50
asthmatic lung ODO4928	29325	40	1.75	3.49	asthmatic lung	1.50
endo cells	control	40	1.75	1.75	endo cells
endo VEGF		40	1.75	1.75	endo VEGF	1.00
endo bFGF		37.41	5.95	5.95	endo bFGF	3.41
heart Clontech	normal	40	1.75	3.49	heart
heart (T-1) ischemic	29417	40	1.75	3.49	heart (T-1)	1.00
					ischemic
heart (T-14) non-	29422	40	1.75	3.49	heart (T-14) non-	1.00
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	40	1.75	3.49	heart (T-3399)	1.00
					DCM
adenoid GW99-269	26162	40	1.75	3.49	adenoid
tonsil GW98-280	22582	35.4	16.36	32.72	tonsil
T cells PC00314	28453	40	1.75	3.49	T cells
PBMNC		40	1.75	1.75	PBMNC
monocyte		40	1.75	3.49	monocyte
B cells PC00665	28455	38.05	4.36	8.72	B cells
dendritic cells	28441	40	1.75	3.49	dendritic cells
neutrophils	28440	40	1.75	1.75	neutrophils
eosinophils	28446	40	1.75	3.49	eosinophils
BM unstim		40	1.75	1.75	BM unstim
BM stim	treated	40	1.75	1.75	BM stim	1.00
osteo dif	treated	40	1.75	1.75	osteo dif	1.00
osteo undif		40	1.75	1.75	osteo undif
chondrocytes		40	1.75	4.36	chondrocytes
OA Synovium IP12/01	29462	35.9	12.65	12.65	OA Synovium
OA Synovium NP10/01	29461	40	1.75	3.49	OA Synovium
OA Synovium NP57/00	28464	34.81	22.26	44.51	OA Synovium
RA Synovium	28466	40	1.75	3.49	RA Synovium
NP03/01
RA Synovium	28467	36.39	9.87	19.74	RA Synovium
NP71/00
RA Synovium	28475	40	1.75	3.49	RA Synovium
NP45/00
OA bone (biobank)	29217	40	1.75	1.75	OA bone (biobank)
OA bone Sample 1	J. Emory	40	1.75	3.49	OA bone
OA bone Sample 2	J. Emory	36.13	11.26	22.51	OA bone
Cartilage (pool)	Normal	36.51	9.29	18.59	Nml Cartilage
					(pool)
Cartilage (pool)	OA	40	1.75	3.49	OA Cartilage (pool)	−5.32
PBL unifected	28441	40	1.75	3.49	PBL unifected
PBL HIV IIIB	28442	40	1.75	3.49	PBL HIV IIIB	1.00
MRC5 uninfected	29158	38.07	4.32	8.64	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	32.86	63.97	127.95	MRC5 HSV strain F	14.82
W12 cells	29179	40	1.75	3.49	W12 cells	1.00
Keratinocytes	29180	40	1.75	3.49	Keratinocytes
B-actin control		27.57	1593.28
genomic		28.2	1053.33
1.00E+05		40	1.75		Disease plate did not have
					genomic samples.
1.00E+05		40	1.75		Copy number was, therefore
					calculated from the gene-
					specific genomic curve on the
					normal plate.
1.00E+04		40	1.75
1.00E+04		40	1.75
1.00E+03		40	1.75
1.00E+03		40	1.75
1.00E+02		40	1.75
1.00E+02		40	1.75
1.00E+01		40	1.75
1.00E+01		40	1.75
1.00E+00		40	1.75
1.00E+00		40	1.75
NTC		40	1.75
NTC		40	1.75
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0158] Gene Name sbg1525809WNT8

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.55
colon tumor                      3.65
colon tumor                      −4.64
colon tumor                      −5.64
lung tumor                       −1.51
lung tumor                       2.38
lung tumor                       1.68
lung tumor                       −3.44
breast tumor                     58.73
breast tumor                     2.63
breast tumor                     2.08
breast tumor                     −8.61
brain stage 5 ALZ                1.27
brain stage 5 ALZ                1.71
brain stage 5 ALZ                1.30
brain stage 5 ALZ                2.49
lung 24 COPD                     −1.33
lung 28 COPD                     −1.33
lung 23 COPD                     −1.33
asthmatic lung                   1.47
asthmatic lung                   1.50
asthmatic lung                   1.50
asthmatic lung                   1.50
endo VEGF                        1.00
endo bFGF                        3.41
heart (T-1) ischemic             1.00
heart (T-14) non-obstructive DCM 1.00
heart (T-3399) DCM               1.00
BM stim                          1.00
osteo dif                        1.00
OA Cartilage (pool)              −5.32
PBLHIV IIIB                      1.00
MRC5 HSV strain F                14.82
W12 cells                        1.00

[0159] Gene Name sbg1519904cdk

[0160] Moderate to high overall expression. The highest normal expression is seen in the whole brain, fetal liver, and thymus. Additional high expression is seen in the subcutaneous adipocytes, the salivary gland and many of the tissues representing the female reproductive system including the mammary gland, the placenta, and the myometrium. This gene is expressed fairly ubiquitously in the disease samples with the highest expression seen in the T cells, uninfected PBLS, and the W12 cells. Upregulation in 2 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in 1 of 4 asthmatic lung samples indicates a possible involvement in asthma Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulated in the stimulated bone marrow and the differentiated osteoblasts. May be implicated in diseases of the bone such as osteoporosis. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1519904cdk	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	31.33, 31.31	70.47	71.37	70.92	3.06	16.34	1158.82
Adipocytes Zenbio
Subcutaneous Adipose	38.32, 35.71	1.13	5.28	3.21	0.96	52.36	167.80
Zenbio
Adrenal Gland Clontech	38.53, 35.13	1	7.43	4.22	0.61	81.97	345.49
Whole Brain Clontech	23.3, 23.29	8125.17	8212.8	8168.99	7.24	6.91	56415.64
Fetal Brain Clontech	40, 37.83	0	1.51	0.76	0.48	103.95	78.48
Cerebellum Clontech	33.54, 32.7	19	31.38	25.19	2.17	23.04	580.41
Cervix	39.09, 34.53	0.71	10.59	5.65	2.42	20.66	116.74
Colon	32.65, 33.21	32.3	23.22	27.76	2.71	18.45	512.18
Endometrium	40, 35.31	0	6.67	3.34	0.73	68.21	227.49
Esophagus	33.86, 34.19	15.77	12.95	14.36	1.37	36.50	524.09
Heart Clontech	35.18, 40	7.23	0	3.62	1.32	37.88	136.93
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	40, 34.3	0	12.17	6.09	2.58	19.38	117.93
Jejunum	31.16, 32.16	77.78	42.98	60.38	6.60	7.58	457.42
Kidney	34.24, 35.35	12.57	6.54	9.56	2.12	23.58	225.35
Liver	35.96, 40	4.56	0	2.28	1.50	33.33	76.00
Fetal Liver Clontech	27.16, 27.02	828.81	901.45	865.13	10.40	4.81	4159.28
Lung	33.27, 32.96	22.35	26.8	24.58	2.57	19.46	478.11
Mammary Gland	29.23, 28.79	243.81	317.27	280.54	13.00	3.85	1079.00
Clontech
Myometrium	33.91, 32.24	15.32	41.01	28.17	2.34	21.37	601.82
Omentum	40, 33.65	0	17.84	8.92	3.94	12.69	113.20
Ovary	33.95, 33.51	14.91	19.39	17.15	4.34	11.52	197.58
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	32.16, 31.84	43.01	52.19	47.60	5.48	9.12	434.31
Placenta Clontech	31.2, 31.32	76.24	70.84	73.54	5.26	9.51	699.05
Prostate	34.02, 33.02	14.34	25.92	20.13	3.00	16.67	335.50
Rectum	34.27, 37.67	12.38	1.66	7.02	1.23	40.65	285.37
Salivary Gland Clontech	30.75, 30.63	99.33	106.86	103.10	7.31	6.84	705.16
Skeletal Muscle	36.12, 36.3	4.13	3.73	3.93	1.26	39.68	155.95
Clontech
Skin	35.5, 34.03	5.99	14.23	10.11	1.21	41.32	417.77
Small Intestine Clontech	35.23, 39.47	7.02	0.57	3.80	0.98	51.07	193.82
Spleen	34.43, 35.07	11.28	7.71	9.50	4.92	10.16	96.49
Stomach	35.12, 40	7.5	8.77	8.14	2.73	18.32	148.99
Testis Clontech	40, 39.08	0	0.72	0.36	0.57	87.87	31.63
Thymus Clontech	27.04, 26.44	893.2	1274.29	1083.75	9.89	5.06	5478.99
Thyroid	35.48, 33.05	6.03	25.39	15.71	2.77	18.05	283.57
Trachea Clontech	30.24, 30.39	134.17	122.65	128.41	9.71	5.15	661.23
Urinary Bladder	40, 32.22	0	41.7	20.85	5.47	9.14	190.59
Uterus	32.01, 33.06	47.08	25.38	36.23	5.34	9.36	339.23
genomic	26.3	1379.25
b-actin	27.49	683.1
1.00E+05	19.36	100000
1.00E+05	19.42	100000
1.00E+04	22.84	10000
1.00E+04	22.8	10000
1.00E+03	26.49	1000
1.00E+03	26.45	1000
1.00E+02	31.23	100
1.00E+02	29.95	100
1.00E+01	33.93	10
1.00E+01	35.99	10
1.00E+00	40	1
1.00E+00	40	0
NTC	40	−1
NTC	37.4	−1
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1519904cdk	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	25.08	2125.15	4250.29	colon normal
colon tumor GW98-166	21940	25.01	2223.66	4447.32	colon tumor	1.05
colon normal GW98-178	22080	27.63	442.18	884.35	colon normal
colon tumor GW98-177	22060	26.06	1141.56	2283.13	colon tumor	2.58
colon normal GW98-561	23514	26.26	1008.48	2016.97	colon normal
colon tumor GW98-560	23513	26.24	1021.02	2042.04	colon tumor	1.01
colon normal GW98-894	24691	25.22	1941.75	3883.51	colon normal
colon tumor GW98-893	24690	25.04	2180.86	4361.72	colon tumor	1.12
lung normal GW98-3	20742	25.13	2057.62	4115.24	lung normal
lung tumor GW98-2	20741	27.03	631.22	1262.44	lung tumor	−3.26
lung normal GW97-179	20677	24.81	2532.80	5065.59	lung normal
lung tumor GW97-178	20676	25.16	2018.20	4036.41	lung tumor	−1.25
lung normal GW98-165	21922	25	2238.12	4476.25	lung normal
lung tumor GW98-164	21921	24.61	2887.95	5775.90	lung tumor	1.29
lung normal GW98-282	22584	26.08	1127.45	2254.91	lung normal
lung tumor GW98-281	22583	26.67	784.49	1568.98	lung tumor	−1.44
breast normal GW00-392	28750	25.78	1360.07	1360.07	breast normal
breast tumor GW00-391	28746	25.58	1543.11	3086.23	breast tumor	2.27
breast normal GW00-413	28798	28.12	332.52	332.52	breast normal
breast tumor GW00-412	28797	26.28	996.11	1992.23	breast tumor	5.99
breast normal GW00-	27592-95	28.65	245.67	245.67	breast normal
235:238
breast tumor GW00-	27588-91	25.79	1351.55	1351.55	breast tumor	5.50
231:234
breast normal GW98-621	23656	25.15	2031.25	4062.51	breast normal
breast tumor GW98-620	23655	24.59	2926.27	5852.54	breast tumor	1.44
brain normal BB99-542	25507	24.69	2739.93	5479.85	brain normal
brain normal BB99-406	25509	26.27	1002.28	2004.56	brain normal
brain normal BB99-904	25546	26.21	1040.13	2080.26	brain normal
brain stage 5 ALZ BB99-	25502	27.76	409.77	819.55	brain stage 5 ALZ	−3.89
874
brain stage 5 ALZ BB99-	25503	24.73	2668.95	5337.90	brain stage 5 ALZ	1.67
887
brain stage 5 ALZ BB99-	25504	25.54	1582.77	3165.53	brain stage 5 ALZ	−1.01
862
brain stage 5 ALZ BB99-	25542	25.72	1412.43	2824.86	brain stage 5 ALZ	−1.13
927
CT lung	normal	26.28	996.11	1992.23	CT lung Nml
lung 26	normal	31.37	56.46		lung 26 Nml
lung 27	normal	31.95	41.95	41.95	lung 27 Nml
lung 24	COPD	33.17	22.85	22.85	lung 24 COPD	−30.21
lung 28	COPD	31.09	65.29	65.29	lung 28 COPD	−10.57
lung 23	COPD	31.27	59.46	59.46	lung 23 COPD	−11.61
lung 25	normal	32.21	36.79	36.79	lung 25 Nml
asthmatic lung ODO3112	29321	27.83	393.38	393.38	asthmatic lung	−1.75
asthmatic lung ODO3433	29323	26.47	886.31	1772.62	asthmatic lung	2.57
asthmatic lung ODO3397	29322	25.24	1916.96	3833.92	asthmatic lung	5.55
asthmatic lung ODO4928	29325	26.5	870.18	1740.37	asthmatic lung	2.52
endo cells	control	29.11	189.76	189.76	endo cells
endo VEGF		29.15	185.58	185.58	endo VEGF	−1.02
endo bFGF		28.83	221.95	221.95	endo bFGF	1.17
heart Clontech	normal	27.41	503.37	1006.73	heart
heart (T-1) ischemic	29417	25.9	1261.43	2522.86	heart (T-1)	2.51
					ischemic
heart (T-14) non-	29422	25.73	1403.56	2807.12	heart (T-14) non-	2.79
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	25.98	1199.91	2399.82	heart (T-3399)	2.38
					DCM
adenoid GW99-269	26162	27.36	518.49	1036.98	adenoid
tonsil GW98-280	22582	25.81	1334.67	2669.33	tonsil
T cells PC00314	28453	24.02	4280.04	8560.08	T cells
PBMNC		33.82	16.68	16.68	PBMNC
monocyte		32.42	33.11	66.21	monocyte
B cells PC00665	28455	25.34	1797.92	3595.85	B cells
dendritic cells	28441	26.54	849.16	1698.33	dendritic cells
neutrophils	28440	24.97	2282.12	2282.12	neutrophils
eosinophils	28446	26.2	1046.59	2093.17	eosinophils
BM unstim		30.6	84.47	84.47	BM unstim
BM stim	treated	28.86	218.24	218.24	BM stim	2.58
osteo dif	treated	29.59	145.56	145.56	osteo dif	2.53
osteo undif		31.33	57.64	57.64	osteo undif
chondrocytes		25.51	1613.22	4033.04	chondrocytes
OA Synovium IP12/01	29462	26.08	1127.45	1127.45	OA Synovium
OA Synovium NP10/01	29461	27.14	591.00	1181.99	OA Synovium
OA Synovium NP57/00	28464	25.83	1318.01	2636.02	OA Synovium
RA Synovium	28466	26.05	1148.69	2297.38	RA Synovium
NP03/01
RA Synovium	28467	25.51	1613.22	3226.43	RA Synovium
NP71/00
RA Synovium	28475	25.74	1394.74	2789.49	RA Synovium
NP45/00
OA bone (biobank)	29217	27.53	468.95	468.95	OA bone (biobank)
OA bone Sample 1	J. Emory	26.81	720.65	1441.29	OA bone
OA bone Sample 2	J. Emory	26.87	695.00	1389.99	OA bone
Cartilage (pool)	Normal	26.02	1170.35	2340.70	Nml Cartilage
					(pool)
Cartilage (pool)	OA	27.15	587.48	1174.95	OA Cartilage	−1.99
					(pool)
PBL unifected	28441	23.67	5429.99	10859.98	PBL unifected
PBL HIV IIIB	28442	25.5	1623.50	3247.01	PBL HIV IIIB	−3.34
MRC5 uninfected	29158	25.69	1439.41	2878.82	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	29.72	135.57	271.15	MRC5 HSV strain F	−10.62
W12 cells	29179	24.11	4028.24	8056.48	W12 cells	1.88
Keratinocytes	29180	25.07	2138.93	4277.86	Keratinocytes
B-actin control		27.39	509.36
genomic		26.99	646.56
1.00E+05		38.65	1.92	Disease plate did not have genomic
				samples.
1.00E+05		38.29	2.23	Copy number was, therefore calculated
				from the gene-specific genomic curve
				on the normal plate.
1.00E+04		36.45	4.95
1.00E+04		38.78	1.81
1.00E+03		38.22	2.30
1.00E+03		39.34	1.44
1.00E+02		39.78	1.20
1.00E+02		39.65	1.27
1.00E+01		36.39	5.09
1.00E+01		39.94	1.12
1.00E+00		39.12	1.57
1.00E+00		38.04	2.48
NTC		40	1.10
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0161] Gene Name sbg1519904cdk

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.05
colon tumor                      2.58
colon tumor                      1.01
colon tumor                      1.12
lung tumor                       −3.26
lung tumor                       −1.25
lung tumor                       1.29
lung tumor                       −1.44
breast tumor                     2.27
breast tumor                     5.99
breast tumor                     5.50
breast tumor                     1.44
brain stage 5 ALZ                −3.89
brain stage 5 ALZ                1.67
brain stage 5 ALZ                −1.01
brain stage 5 ALZ                −1.13
lung 24 COPD                     −30.21
lung 28 COPD                     −10.57
lung 23 COPD                     −11.61
asthmatic lung                   −1.75
asthmatic lung                   2.57
asthmatic lung                   5.55
asthmatic lung                   2.52
endo VEGF                        −1.02
endo bFGF                        1.17
heart (T-1) ischemic             2.51
heart (T-14) non-obstructive DCM 2.79
heart (T-3399) DCM               2.38
BM stim                          2.58
osteo dif                        2.53
OA Cartilage (pool)              −1.99
PBL HIV IIIB                     −3.34
MRC5 HSV strain F                −10.62
W12 cells                        1.88

[0162] Gene Name sbg1521449connexin

[0163] Moderate to low overall expression. This gene is expressed fairly ubiquitously in the normal samples with highest levels of expression seen in the whole brain, hypothalamus, liver, fetal liver, and thymus. The highest disease expression is seen in the breast, colon, and lung normal and tumor samples and in the normal and disease brain samples. Upregulation in 2 of 4 breast tumor samples implicates this gene in breast cancer. Downregulation in 2 of 4 asthmatic lung samples indicates a possible involvement in asthma Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Moderate to high expression in the RA and OA synovium samples and the chondrocytes with corroborating high expression in the T cells, B cells, dendritic cells, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1521449-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
connexin	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	38.05, 37.25	0.37	0.62	0.50	3.06	16.34	8.09
Adipocytes Zenbio
Subcutaneous Adipose	39.32, 39.13	0.16	0.18	0.17	0.96	52.36	8.90
Zenbio
Adrenal Gland Clontech	37.27, 36.02	0.61	1.4	1.01	0.61	81.97	82.38
Whole Brain Clontech	26.93, 26.74	579.86	654.12	616.99	7.24	6.91	4260.98
Fetal Brain Clontech	38.44, 36.08	0.28	1.35	0.82	0.48	103.95	84.72
Cerebellum Clontech	34.17, 33.86	4.77	5.89	5.33	2.17	23.04	122.81
Cervix	35.78, 34.45	1.64	3.97	2.81	2.42	20.66	57.95
Colon	37.58, 34.4	0.5	4.1	2.30	2.71	18.45	42.44
Endometrium	35.52, 36.32	1.95	1.15	1.55	0.73	68.21	105.73
Esophagus	37.2, 36.68	0.64	0.91	0.78	1.37	36.50	28.28
Heart Clontech	35.58, 35.61	1.88	1.84	1.86	1.32	37.88	70.45
Hypothalamus	35.78, 36.24	1.64	1.22	1.43	0.32	155.28	222.05
Ileum	36.89, 35.46	0.79	2.03	1.41	2.58	19.38	27.33
Jejunum	32.55, 32.37	13.94	15.76	14.85	6.60	7.58	112.50
Kidney	36.69, 36.32	0.9	1.15	1.03	2.12	23.58	24.17
Liver	33.4, 34.37	7.97	4.18	6.08	1.50	33.33	202.50
Fetal Liver Clontech	31.75, 31.56	23.79	26.88	25.34	10.40	4.81	121.80
Lung	35.02, 35.59	2.72	1.86	2.29	2.57	19.46	44.55
Mammary Gland	32.27, 32.71	16.81	12.59	14.70	13.00	3.85	56.54
Clontech
Myometrium	35.06, 35.53	2.65	1.94	2.30	2.34	21.37	49.04
Omentum	32.33, 34.21	16.17	4.66	10.42	3.94	12.69	132.17
Ovary	33.74, 33.59	6.35	7	6.68	4.34	11.52	76.90
Pancreas	36.87, 36.58	0.8	0.97	0.89	0.81	61.80	54.70
Head of Pancreas	34.28, 36.68	4.46	0.91	2.69	1.57	31.85	85.51
Parotid Gland	34.09, 33.54	5.03	7.25	6.14	5.48	9.12	56.02
Placenta Clontech	35.07, 36.04	2.63	1.39	2.01	5.26	9.51	19.11
Prostate	35.42, 35.23	2.1	2.37	2.24	3.00	16.67	37.25
Rectum	36.31, 35.93	1.16	1.49	1.33	1.23	40.65	53.86
Salivary Gland Clontech	34.01, 33.49	5.3	7.48	6.39	7.31	6.84	43.71
Skeletal Muscle	37.12, 38.72	0.68	0.23	0.46	1.26	39.68	18.06
Clontech
Skin	39.2, 37.09	0.17	0.69	0.43	1.21	41.32	17.77
Small Intestine Clontech	38.84, 37.18	0.22	0.65	0.44	0.98	51.07	22.22
Spleen	34.52, 34.67	3.79	3.43	3.61	4.92	10.16	36.69
Stomach	36.3, 35.98	1.16	1.44	1.30	2.73	18.32	23.81
Testis Clontech	37.29, 36.56	0.61	0.98	0.80	0.57	87.87	69.86
Thymus Clontech	31.3, 31.11	31.97	36.26	34.12	9.89	5.06	172.47
Thyroid	35.22, 36.32	2.39	1.15	1.77	2.77	18.05	31.95
Trachea Clontech	34.39, 34.02	4.13	5.27	4.70	9.71	5.15	24.20
Urinary Bladder	34.87, 34.25	3.01	4.53	3.77	5.47	9.14	34.46
Uterus	33.4, 32.98	7.98	10.51	9.25	5.34	9.36	86.56
genomic	25.67	1329.61
b-actin	27.33	444.52
1.00E+05	19.28	100000
1.00E+05	19.4	100000
1.00E+04	22.27	10000
1.00E+04	22.19	10000
1.00E+03	26.01	1000
1.00E+03	25.8	1000
1.00E+02	30.08	100
1.00E+02	29.91	100
1.00E+01	33.07	10
1.00E+01	33.84	10
1.00E+00	37.02	1
1.00E+00	35.22	1	−
NTC	38.14	−1
NTC	37.85	−1
				copies of
				mRNA		Fold
	Reg			detected/		Change
Sample	number		Mean	50 ng		in
sbg1521449-	(GSK		GOI	total		Disease
connexin	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	29.61	127.84	255.68	colon normal
colon tumor GW98-166	21940	29.7	120.56	241.12	colon tumor	−1.06
colon normal GW98-178	22080	31.16	47.88	95.76	colon normal
colon tumor GW98-177	22060	30.04	97.35	194.70	colon tumor	2.03
colon normal GW98-561	23514	30.47	74.05	148.10	colon normal
colon tumor GW98-560	23513	30.59	68.46	136.92	colon tumor	−1.08
colon normal GW98-894	24691	29.36	148.99	297.98	colon normal
colon tumor GW98-893	24690	29.27	158.62	317.24	colon tumor	1.06
lung normal GW98-3	20742	29.04	182.72	365.44	lung normal
lung tumor GW98-2	20741	31.3	43.67	87.34	lung tumor	−4.18
lung normal GW97-179	20677	30.21	87.43	174.86	lung normal
lung tumor GW97-178	20676	29.38	147.55	295.10	lung tumor	1.69
lung normal GW98-165	21922	29.52	135.41	270.82	lung normal
lung tumor GW98-164	21921	30.07	95.25	190.50	lung tumor	−1.42
lung normal GW98-282	22584	31.11	49.34	98.68	lung normal
lung tumor GW98-281	22583	30.77	61.11	122.22	lung tumor	1.24
breast normal GW00-392	28750	29.34	151.22	151.22	breast normal
breast tumor GW00-391	28746	28.76	217.94	435.88	breast tumor	2.88
breast normal GW00-413	28798	30.46	74.23	74.23	breast normal
breast tumor GW00-412	28797	29.03	184.33	368.66	breast tumor	4.97
breast normal GW00-	27592-95	33.21	13	13.00	breast normal
235:238
breast tumor GW00-	27588-91	30.17	89.28	89.28	breast tumor	6.87
231:234
breast normal GW98-621	23656	29.48	138.9	277.80	breast normal
breast tumor GW98-620	23655	29.99	100.55	201.10	breast tumor	−1.38
brain normal BB99-542	25507	30.6	68.32	136.64	brain normal
brain normal BB99-406	25509	30.74	62.32	124.64	brain normal
brain normal BB99-904	25546	31.09	50.01	100.02	brain normal
brain stage 5 ALZ BB99-	25502	31.03	51.88	103.76	brain stage 5 ALZ	−1.16
874
brain stage 5 ALZ BB99-	25503	29.5	137	274.00	brain stage 5 ALZ	2.28
887
brain stage 5 ALZ BB99-	25504	30.04	97.32	194.64	brain stage 5 ALZ	1.62
862
brain stage 5 ALZ BB99-	25542	30.12	92.58	185.16	brain stage 5 ALZ	1.54
927
CT lung	normal	31.49	38.73	77.46	CT lung Nml
lung 26	normal	34.07	7.55		lung 26 Nml
lung 27	normal	38.07	0.6	0.60	lung 27 Nml
lung 24	COPD	37.74	0.74	0.74	lung 24 COPD	−36.11
lung 28	COPD	36.85	1.3	1.30	lung 28 COPD	−20.55
lung 23	COPD	36.02	2.19	2.19	lung 23 COPD	−12.20
lung 25	normal	36.09	2.1	2.10	lung 25 Nml
asthmatic lung ODO3112	29321	35.54	2.98	2.98	asthmatic lung	−8.97
asthmatic lung ODO3433	29323	34.42	6.05	12.10	asthmatic lung	−2.21
asthmatic lung ODO3397	29322	31.9	29.9	59.80	asthmatic lung	2.24
asthmatic lung ODO4928	29325	33.84	8.75	17.50	asthmatic lung	−1.53
endo cells	control	34.17	7.08	7.08	endo cells
endo VEGF		34.73	4.96	4.96	endo VEGF	−1.43
endo bFGF		34.51	5.71	5.71	endo bFGF	−1.24
heart Clontech	normal	35.07	4.01	8.02	heart
heart (T-1) ischemic	29417	32.02	27.63	55.26	heart (T-1)	6.89
					ischemic
heart (T-14) non-	29422	32.12	26.01	52.02	heart (T-14) non-	6.49
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	31.81	31.6	63.20	heart (T-3399)	7.88
					DCM
adenoid GW99-269	26162	32.06	27.08	54.16	adenoid
tonsil GW98-280	22582	31.65	35.03	70.06	tonsil
T cells PC00314	28453	29.97	101.75	203.50	T cells
PBMNC		35.68	2.72	2.72	PBMNC
monocyte		33.68	9.64	19.28	monocyte
B cells PC00665	28455	29.93	104.12	208.24	B cells
dendritic cells	28441	30.83	58.85	117.70	dendritic cells
neutrophils	28440	31.1	49.65	49.65	neutrophils
eosinophils	28446	31.01	52.56	105.12	eosinophils
BM unstim		35.46	3.13	3.13	BM unstim
BM stim	treated	34.87	4.55	4.55	BM stim	1.45
osteo dif	treated	36.01	2.2	2.20	osteo dif	1.95
osteo undif		37.06	1.13	1.13	osteo undif
chondrocytes		31.4	40.95	102.38	chondrocytes
OA Synovium IP12/01	29462	34.18	7.04	7.04	OA Synovium
OA Synovium NP10/01	29461	32.08	26.64	53.28	OA Synovium
OA Synovium NP57/00	28464	31.17	47.32	94.64	OA Synovium
RA Synovium	28466	31.12	49.1	98.20	RA Synovium
NP03/01
RA Synovium	28467	30.48	73.65	147.30	RA Synovium
NP71/00
RA Synovium	28475	31.52	37.97	75.94	RA Synovium
NP45/00
OA bone (biobank)	29217	33.84	8.73	8.73	OA bone (biobank)
OA bone Sample 1	J. Emory	33.02	14.69	29.38	OA bone
OA bone Sample 2	J. Emory	34	7.91	15.82	OA bone
Cartilage (pool)	Normal	32.41	21.57	43.14	Nml Cartilage
					(pool)
Cartilage (pool)	OA	33.21	13.01	26.02	OA Cartilage (pool)	−1.66
PBL unifected	28441	31.16	47.76	95.52	PBL unifected
PBL HIV IIIB	28442	31.06	50.89	101.78	PBL HIV IIIB	1.07
MRC5 uninfected	29158	31.11	49.19	98.38	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	32.04	27.34	54.68	MRC5 HSV strain F	−1.80
W12 cells	29179	31.48	39.06	78.12	W12 cells	1.37
Keratinocytes	29180	31.98	28.42	56.84	Keratinocytes
B-actin control		27.14	609.73
genomic		25.24	2031.4
1.00E+05		19.93	100000
1.00E+05		19.87	100000
1.00E+04		22.49	10000
1.00E+04		22.37	10000
1.00E+03		24.73	1000
1.00E+03		24.83	1000
1.00E+02		30.26	100
1.00E+02		30.17	100
1.00E+01		35.69	10
1.00E+01		34.47	10
1.00E+00		37.49	1
1.00E+00		35.83	1
NTC		37.35	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0164] Gene Name sbg1521449connexin

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.06
colon tumor                      2.03
colon tumor                      −1.08
colon tumor                      1.06
lung tumor                       −4.18
lung tumor                       1.69
lung tumor                       −1.42
lung tumor                       1.24
breast tumor                     2.88
breast tumor                     4.97
breast tumor                     6.87
breast tumor                     −1.38
brain stage 5 ALZ                −1.16
brain stage 5 ALZ                2.28
brain stage 5 ALZ                1.62
brain stage 5 ALZ                1.54
lung 24 COPD                     −36.11
lung 28 COPD                     −20.55
lung 23 COPD                     −12.20
asthmatic lung                   −8.97
asthmatic lung                   −2.21
asthmatic lung                   2.24
asthmatic lung                   −1.53
endo VEGF                        −1.43
endo bFGF                        −1.24
heart (T-1) ischemic             6.89
heart (T-14) non-obstructive DCM 6.49
heart (T-3399) DCM               7.88
BM stim                          1.45
osteo dif                        1.95
OA Cartilage (pool)              −1.66
PBL HIV IIIB                     1.07
MRC5 HSV strain F                −1.80
W12 cells                        1.37

[0165] Gene Name sbg1522659Siglec (Taqman was Performed for this Sample)

[0166] Moderate to high overall expression. The highest normal expression is seen in the myometrium, ovary, adrenal gland, whole brain, spleen, and fetal liver. The other tissues representing both the GI tract and the female reproductive system also show high expression. These tissues include the jejunum, omentum, small intestine, rectum, stomach, endometrium, and placenta. The GI pattern of expression suggests that this gene may be involved in diseases of the digestive tract such as IBS and Crohn's Disease. The highest disease expression is seen in the Clontech normal lung sample, some of the asthmatic lung samples, the Clontech normal heart sample, and all of the OA samples. Upregulation in 2 of 4 lung tumor samples implicates this gene in lung cancer. Downregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulated in the stimulated bone marrow sample. Downregulation in the OA cartilage pool suggests that this gene may be involved in or a consequence of osteoarthritis. Upregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV. Upregulation in the W12 cells suggests that this gene may be a host factor in HPV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1522659Siglec	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	32.79, 32.97	0.26	0.22	0.24	3.06	16.34	3.92
Adipocytes Zenbio
Subcutaneous Adipose	32.36, 32.09	0.41	0.54	0.48	0.96	52.36	24.87
Zenbio
Adrenal Gland	30.43, 30.5	2.82	2.63	2.73	0.61	81.97	223.36
Clontech
Whole Brain Clontech	26.61, 26.6	127.82	129.82	128.82	7.24	6.91	889.64
Fetal Brain Clontech	27.17, 30.55	72.98	2.49	37.74	0.48	103.95	3922.56
Cerebellum Clontech	31.1, 31.54	1.44	0.93	1.19	2.17	23.04	27.30
Cervix	31.19, 31.05	1.32	1.51	1.42	2.42	20.66	29.24
Colon	30.75, 30.9	2.04	1.75	1.90	2.71	18.45	34.96
Endometrium	30.47, 30.71	2.71	2.12	2.42	0.73	68.21	164.73
Esophagus	30.06, 30.12	4.07	3.82	3.95	1.37	36.50	143.98
Heart Clontech	30.11, 30.33	3.88	3.11	3.50	1.32	37.88	132.39
Hypothalamus	30.08, 30.31	3.99	3.16	3.58	0.32	155.28	555.12
Ileum	31.29, 31.04	1.19	1.53	1.36	2.58	19.38	26.36
Jejunum	31.07, 31.19	1.48	1.31	1.40	6.60	7.58	10.57
Kidney	30.45, 30.84	2.75	1.87	2.31	2.12	23.58	54.48
Liver	30.2, 30.13	3.53	3.79	3.66	1.50	33.33	122.00
Fetal Liver Clontech	29.09, 28.81	10.71	14.2	12.46	10.40	4.81	59.88
Lung	30.77, 31.04	2	1.53	1.77	2.57	19.46	34.34
Mammary Gland	31.23, 31.23	1.26	1.26	1.26	13.00	3.85	4.85
Clontech
Myometrium	30.34, 30.99	3.09	1.61	2.35	2.34	21.37	50.21
Omentum	31.03, 31.02	1.54	1.55	1.55	3.94	12.69	19.61
Ovary	28.69, 28.77	16.06	14.84	15.45	4.34	11.52	178.00
Pancreas	30.57, 30.38	2.45	2.95	2.70	0.81	61.80	166.87
Head of Pancreas	30.22, 30.97	3.45	1.63	2.54	1.57	31.85	80.89
Parotid Gland	31.25, 31.06	1.24	1.5	1.37	5.48	9.12	12.50
Placenta Clontech	30.75, 30.63	2.05	2.31	2.18	5.26	9.51	20.72
Prostate	30.45, 30.59	2.75	2.39	2.57	3.00	16.67	42.83
Rectum	30.3, 30.68	3.21	2.2	2.71	1.23	40.65	109.96
Salivary Gland	30.6, 31.04	2.36	1.53	1.95	7.31	6.84	13.30
Clontech
Skeletal Muscle	30.82, 31.03	1.9	1.55	1.73	1.26	39.68	68.45
Clontech
Skin	30.65, 30.33	2.25	3.11	2.68	1.21	41.32	110.74
Small Intestine	30.61, 30.64	2.35	2.29	2.32	0.98	51.07	118.49
Clontech
Spleen	30.6, 30.7	2.38	2.14	2.26	4.92	10.16	22.97
Stomach	30.77, 30.97	2.01	1.63	1.82	2.73	18.32	33.33
Testis Clontech	30.26, 30.18	3.32	3.61	3.47	0.57	87.87	304.48
Thymus Clontech	28.57, 28.75	17.98	15.05	16.52	9.89	5.06	83.49
Thyroid	31, 30.75	1.59	2.03	1.81	2.77	18.05	32.67
Trachea Clontech	31.06, 30.99	1.49	1.6	1.55	9.71	5.15	7.96
Urinary Bladder	30.92, 30.81	1.72	1.93	1.83	5.47	9.14	16.68
Uterus	30.4, 30.95	2.89	1.66	2.28	5.34	9.36	21.30
genomic	24.94	680.71
b-actin	27.61	47.14
1.00E+05	19.42	100000
1.00E+05	19.39	100000
1.00E+04	22.09	10000
1.00E+04	21.97	10000
1.00E+03	24.5	1000
1.00E+03	24.43	1000
1.00E+02	28.5	100
1.00E+02	28.27	100
1.00E+01	28.83	10
1.00E+01	31.14	10
1.00E+00	29.91	1
1.00E+00	30.02	1
NTC	29.79	−1
NTC	30.05	−1
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
sbg1522659Siglec	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	28.59	33.24	66.48	colon normal
colon tumor GW98-166	21940	28.78	27.91	55.82	colon tumor	−1.19
colon normal GW98-178	22080	30.22	7.7	15.40	colon normal
colon tumor GW98-177	22060	29.4	16.01	32.02	colon tumor	2.08
colon normal GW98-561	23514	30.01	9.27	18.54	colon normal
colon tumor GW98-560	23513	30.15	8.14	16.28	colon tumor	−1.14
colon normal GW98-894	24691	28.96	23.84	47.68	colon normal
colon tumor GW98-893	24690	28.44	38.1	76.20	colon tumor	1.60
lung normal GW98-3	20742	29.06	21.79	43.58	lung normal
lung tumor GW98-2	20741	30	9.37	18.74	lung tumor	−2.33
lung normal GW97-179	20677	29.96	9.73	19.46	lung normal
lung tumor GW97-178	20676	27.91	61.4	122.80	lung tumor	6.31
lung normal GW98-165	21922	28.37	40.67	81.34	lung normal
lung tumor GW98-164	21921	29.21	19.05	38.10	lung tumor	−2.13
lung normal GW98-282	22584	30.06	8.88	17.76	lung normal
lung tumor GW98-281	22583	29.11	20.89	41.78	lung tumor	2.35
breast normal GW00-392	28750	29.88	10.38	10.38	breast normal
breast tumor GW00-391	28746	29.83	10.89	21.78	breast tumor	2.10
breast normal GW00-413	28798	31.21	3.16	3.16	breast normal
breast tumor GW00-412	28797	29.86	10.63	21.26	breast tumor	6.73
breast normal GW00-	27592-95	32.1	1.42	1.42	breast normal
235:238
breast tumor GW00-	27588-91	31.73	1.97	1.97	breast tumor	1.39
231:234
breast normal GW98-621	23656	29.11	20.78	41.56	breast normal
breast tumor GW98-620	23655	29.14	20.29	40.58	breast tumor	−1.02
brain normal BB99-542	25507	29.79	11.34	22.68	brain normal
brain normal BB99-406	25509	29.73	11.93	23.86	brain normal
brain normal BB99-904	25546	30.12	8.4	16.80	brain normal
brain stage 5 ALZ BB99-	25502	29.88	10.44	20.88	brain stage 5 ALZ	−1.01
874
brain stage 5 ALZ BB99-	25503	29.07	21.63	43.26	brain stage 5 ALZ	2.05
887
brain stage 5 ALZ BB99-	25504	29.29	17.74	35.48	brain stage 5 ALZ	1.68
862
brain stage 5 ALZ BB99-	25542	29.84	10.8	21.60	brain stage 5 ALZ	1.02
927
CT lung	normal	28.36	40.79	81.58	CT lung Nml
lung 26	normal	28.68	30.74		lung 26 Nml
lung 27	normal	31.5	2.43	2.43	lung 27 Nml
lung 24	COPD	31.84	1.78	1.78	lung 24 COPD	−16.29
lung 28	COPD	31.63	2.16	2.16	lung 28 COPD	−13.42
lung 23	COPD	32.08	1.43	1.43	lung 23 COPD	−20.27
lung 25	normal	31.28	2.96	2.96	lung 25 Nml
asthmatic lung ODO3112	29321	31.03	3.7	3.70	asthmatic lung	−7.84
asthmatic lung ODO3433	29323	30.1	8.53	17.06	asthmatic lung	−1.70
asthmatic lung ODO3397	29322	28.3	43.06	86.12	asthmatic lung	2.97
asthmatic lung ODO4928	29325	29.71	12.09	24.18	asthmatic lung	−1.20
endo cells	control	31.53	2.37	2.37	endo cells
endo VEGF		32.48	1.01	1.01	endo VEGF	−2.35
endo bFGF		31.64	2.13	2.13	endo bFGF	−1.11
heart Clontech	normal	28.78	28.16	56.32	heart
heart (T-1) ischemic	29417	30.04	9.03	18.06	heart (T-1) ischemic	−3.12
heart (T-14) non-	29422	30.52	5.85	11.70	heart (T-14) non-	−4.81
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	30.03	9.1	18.20	heart (T-3399) DCM	−3.09
adenoid GW99-269	26162	30.31	7.05	14.10	adenoid
tonsil GW98-280	22582	29.06	21.74	43.48	tonsil
T cells PC00314	28453	29.07	21.64	43.28	T cells
PBMNC		32.15	1.35	1.35	PBMNC
monocyte		31.25	3.03	6.06	monocyte
B cells PC00665	28455	29.44	15.46	30.92	B cells
dendritic cells	28441	30.62	5.35	10.70	dendritic cells
neutrophils	28440	29.12	20.61	20.61	neutrophils
eosinophils	28446	29.41	15.85	31.70	eosinophils
BM unstim		32.07	1.45	1.45	BM unstim
BM stim	treated	30.78	4.65	4.65	BM stim	3.21
osteo dif	treated	31.61	2.19	2.19	osteo dif	1.58
osteo undif		32.12	1.39	1.39	osteo undif
chondrocytes		30.4	6.55	16.38	chondrocyt s
OA Synovium IP12/01	29462	28.48	36.75	36.75	OA Synovium
OA Synovium NP10/01	29461	29.49	14.84	29.68	OA Synovium
OA Synovium NP57/00	28464	28.59	33.29	66.58	OA Synovium
RA Synovium	28466	28.6	32.84	65.68	RA Synovium
NP03/01
RA Synovium	28467	28.6	32.95	65.90	RA Synovium
NP71/00
RA Synovium	28475	29.19	19.32	38.64	RA Synovium
NP45/00
OA bone (biobank)	29217	28.49	36.53	36.53	OA bone (biobank)
OA bone Sample 1	J. Emory	28.42	38.86	77.72	OA bone
OA bone Sample 2	J. Emory	28.73	29.22	58.44	OA bone
Cartilage (pool)	Normal	29.39	16.21	32.42	Nml Cartilage (pool)
Cartilage (pool)	OA	30.62	5.33	10.66	OA Cartilage (pool)	−3.04
PBL unifected	28441	29.94	9.86	19.72	PBL unifected
PBL HIV IIIB	28442	29.36	16.65	33.30	PBL HIV IIIB	1.69
MRC5 uninfected	29158	30.27	7.36	14.72	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	30.73	4.83	9.66	MRC5 HSV strain F	−1.52
W12 cells	29179	31.09	3.5	7.00	W12 cells	−1.29
Keratinocytes	29180	30.81	4.51	9.02	Keratinocytes
B-actin control		27.35	101.64
genomic		25.51	531.17
1.00E+05		19.06	100000
1.00E+05		19.11	100000
1.00E+04		22.1	10000
1.00E+04		21.85	10000
1.00E+03		25.01	1000
1.00E+03		24.81	1000
1.00E+02		28.64	100
1.00E+02		29.11	100
1.00E+01		30.45	10
1.00E+01		30.95	10
1.00E+00		30.99	1
1.00E+00		30.95	1
NTC		31.42	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0167] Gene Name sbg1522659Siglec

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.19
colon tumor                      2.08
colon tumor                      −1.14
colon tumor                      1.60
lung tumor                       −2.33
lung tumor                       6.31
lung tumor                       −2.13
lung tumor                       2.35
breast tumor                     2.10
breast tumor                     6.73
breast tumor                     1.39
breast tumor                     −1.02
brain stage 5 ALZ                −1.01
brain stage 5 ALZ                2.05
brain stage 5 ALZ                1.68
brain stage 5 ALZ                1.02
lung 24 COPD                     −16.29
lung 28 COPD                     −13.42
lung 23 COPD                     −20.27
asthmatic lung                   −7.84
asthmatic lung                   −1.70
asthmatic lung                   2.97
asthmatic lung                   −1.20
endo VEGF                        −2.35
endo bFGF                        −1.11
heart (T-1) ischemic             −3.12
heart (T-14) non-obstructive DCM −4.81
heart (T-3399) DCM               −3.09
BM stim                          3.21
osteo dif                        1.58
OA Cartilage (pool)              −3.04
PEL HIV IIIB                     1.69
MRC5 HSV strain F                −1.52
W12 cells                        −1.29

[0168] Gene Name sbg1528389ITI

[0169] Moderate overall expression. This gene is expressed fairly ubiquitously in the normal samples with the highest expression seen in the whole brain, lung, thymus, and testis. The highest disease expression is seen in some of the colon, breast, and lung normal and tumor samples as well as in the asthmatic lung samples. Upregulation in 2 of 4 colon tumor samples implicates this gene in colon cancer. Upregulation in 2 of 4 asthmatic lung samples indicates a possible involvement in asthma Upregulation in the HIV-infected PBLs cells suggests that this gene may be a host factor in HIV. Upregulation in the OA cartilage pool and high expression in the RA and OA synovium samples, and the OA bone samples with corroborating high expression in the T cells, B cells, and neutrophils, dendritic cells, and the eosinophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1528389ITI	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 39.7	0	0.43	0.22	3.06	16.34	3.51
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	37.07, 39.08	2.04	0.62	1.33	0.61	81.97	109.02
Whole Brain Clontech	27.35, 27.52	634.74	576.15	605.45	7.24	6.91	4181.25
Fetal Brain Clontech	36.09, 37.27	3.64	1.82	2.73	0.48	103.95	283.78
Cerebellum Clontech	36.66, 36.18	2.6	3.45	3.03	2.17	23.04	69.70
Cervix	38.2, 36.11	1.05	3.6	2.33	2.42	20.66	48.04
Colon	33.09, 32.85	21.4	24.64	23.02	2.71	18.45	424.72
Endometrium	37.25, 34.99	1.84	6.99	4.42	0.73	68.21	301.16
Esophagus	37.06, 36.38	2.05	3.07	2.56	1.37	36.50	93.43
Heart Clontech	37.13, 35.89	1.98	4.1	3.04	1.32	37.88	115.15
Hypothalamus	38.14, 37.58	1.09	1.51	1.30	0.32	155.28	201.86
Ileum	34.17, 34.6	11.31	8.76	10.04	2.58	19.38	194.48
Jejunum	33.32, 32.88	18.73	24.26	21.50	6.60	7.58	162.84
Kidney	36.95, 39.47	2.2	0.49	1.35	2.12	23.58	31.72
Liver	35.02, 35.4	6.87	5.48	6.18	1.50	33.33	205.83
Fetal Liver Clontech	33.53, 33.48	16.53	17.05	16.79	10.40	4.81	80.72
Lung	34.5, 31.28	9.32	62.37	35.85	2.57	19.46	697.37
Mammary Gland	34.17, 33.53	11.34	16.56	13.95	13.00	3.85	53.65
Clontech
Myometrium	34.52, 35.41	9.22	5.44	7.33	2.34	21.37	156.62
Omentum	35.14, 34.07	6.4	12.03	9.22	3.94	12.69	116.94
Ovary	34.02, 34.32	12.37	10.37	11.37	4.34	11.52	130.99
Pancreas	36.86, 37.21	2.31	1.88	2.10	0.81	61.80	129.48
Head of Pancreas	34.54, 35.46	9.09	5.3	7.20	1.57	31.85	229.14
Parotid Gland	32.22, 33.21	35.77	20	27.89	5.48	9.12	254.43
Placenta Clontech	33.64, 34.31	15.47	10.44	12.96	5.26	9.51	123.15
Prostate	35.95, 38.03	3.95	1.16	2.56	3.00	16.67	42.58
Rectum	36.19, 34.63	3.43	8.63	6.03	1.23	40.65	245.12
Salivary Gland Clontech	34.97, 35.39	7.07	5.52	6.30	7.31	6.84	43.06
Skeletal Muscle	36.99, 36.59	2.14	2.71	2.43	1.26	39.68	96.23
Clontech
Skin	34.99, 34.63	6.97	8.63	7.80	1.21	41.32	322.31
Small Intestine Clontech	35.78, 36.83	4.37	2.35	3.36	0.98	51.07	171.60
Spleen	36.13, 37.09	3.56	2.02	2.79	4.92	10.16	28.35
Stomach	35.12, 34.26	6.47	10.73	8.60	2.73	18.32	157.51
Testis Clontech	35.84, 35.35	4.23	5.64	4.94	0.57	87.87	433.66
Thymus Clontech	30.97, 30.87	75.12	79.5	77.31	9.89	5.06	390.85
Thyroid	35.11, 35.5	6.48	5.15	5.82	2.77	18.05	104.96
Trachea Clontech	33.16, 33.71	20.51	14.85	17.68	9.71	5.15	91.04
Urinary Bladder	35.13, 35.13	6.42	6.42	6.42	5.47	9.14	58.68
Uterus	33.91, 33.81	13.18	14.02	13.60	5.34	9.36	127.34
genomic	24.82	2824.82
b-actin	27.4	615.4
1.00E+05	19.68	100000
1.00E+05	19.74	100000
1.00E+04	21.44	10000
1.00E+04	22.63	10000
1.00E+03	26.05	1000
1.00E+03	25.81	1000
1.00E+02	30.64	100
1.00E+02	30.82	100
1.00E+01	33.47	10
1.00E+01	34.14	10
1.00E+00	39.1	1
1.00E+00	38.87	1
NTC	40	−1
NTC	39.62	−1
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
sbg1528389ITI	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	29.14	216.46	432.92	colon normal
colon tumor GW98-166	21940	27.54	550.97	1101.94	colon tumor	2.55
colon normal GW98-178	22080	32.22	35.92	71.84	colon normal
colon tumor GW98-177	22060	27.74	488.73	977.46	colon tumor	13.61
colon normal GW98-561	23514	30.58	93.27	186.54	colon normal
colon tumor GW98-560	23513	26.52	996.72	1993.44	colon tumor	10.69
colon normal GW98-894	24691	29.1	221.71	443.42	colon normal
colon tumor GW98-893	24690	28	420.56	841.12	colon tumor	1.90
lung normal GW98-3	20742	28.35	343.97	687.94	lung normal
lung tumor GW98-2	20741	29.86	141.95	283.90	lung tumor	−2.42
lung normal GW97-179	20677	26.22	1190.76	2381.52	lung normal
lung tumor GW97-178	20676	28.38	337.28	674.56	lung tumor	−3.53
lung normal GW98-165	21922	26.98	764.17	1528.34	lung normal
lung tumor GW98-164	21921	28.26	362.34	724.68	lung tumor	−2.11
lung normal GW98-282	22584	31.43	56.99	113.98	lung normal
lung tumor GW98-281	22583	31.03	72.06	144.12	lung tumor	1.26
breast normal GW00-392	28750	29.1	221.58	221.58	breast normal
breast tumor GW00-391	28746	29.16	213.78	427.56	breast tumor	1.93
breast normal GW00-413	28798	31.29	61.63	61.63	breast normal
breast tumor GW00-412	28797	30.38	105.24	210.48	breast tumor	3.42
breast normal GW00-	27592-95	30.91	76.93	76.93	breast normal
235:238
breast tumor GW00-	27588-91	32.6	28.82	28.82	breast tumor	−2.67
231:234
breast normal GW98-621	23656	31.46	55.99	111.98	breast normal
breast tumor GW98-620	23655	30.46	100.08	200.16	breast tumor	1.79
brain normal BB99-542	25507	31.13	67.76	135.52	brain normal
brain normal BB99-406	25509	30.81	81.84	163.68	brain normal
brain normal BB99-904	25546	32.62	28.45	56.90	brain normal
brain stage 5 ALZ BB99-	25502	31.06	70.8	141.60	brain stage 5 ALZ	1.19
874
brain stage 5 ALZ BB99-	25503	30.4	103.5	207.00	brain stage 5 ALZ	1.74
887
brain stage 5 ALZ BB99-	25504	31.15	67.03	134.06	brain stage 5 ALZ	1.13
862
brain stage 5 ALZ BB99-	25542	31.38	58.64	117.28	brain stage 5 ALZ	−1.01
927
CT lung	normal	29.75	151.95	303.90	CT lung Nml
lung 26	normal	28.26	361.08		lung 26 Nml
lung 27	normal	31.13	67.84	67.84	lung 27 Nml
lung 24	COPD	30.48	99.14	99.14	lung 24 COPD	−1.73
lung 28	COPD	31.22	64.26	64.26	lung 28 COPD	−2.66
lung 23	COPD	33.41	17.89	17.89	lung 23 COPD	−9.56
lung 25	normal	29.87	141.5	141.50	lung 25 Nml
asthmatic lung ODO3112	29321	29.23	205.61	205.61	asthmatic lung	1.20
asthmatic lung ODO3433	29323	29.42	183.77	367.54	asthmatic lung	2.15
asthmatic lung ODO3397	29322	27.81	469.96	939.92	asthmatic lung	5.49
asthmatic lung ODO4928	29325	28.16	382.12	764.24	asthmatic lung	4.47
endo cells	control	35.58	5.07	5.07	endo cells
endo VEGF		36.5	2.95	2.95	endo VEGF	−1.72
endo bFGF		35.2	6.32	6.32	endo bFGF	1.25
heart Clontech	normal	36.64	2.73	5.46	heart
heart (T-1) ischemic	29417	32.68	27.53	55.06	heart (T-1) ischemic	10.08
heart (T-14) non-	29422	39.58	0.49	0.98	heart (T-14) non-	−5.57
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	35.02	7.02	14.04	heart (T-3399) DCM	2.57
adenoid GW99-269	26162	31.63	50.7	101.40	adenoid
tonsil GW98-280	22582	30.69	87.76	175.52	tonsil
T cells PC00314	28453	30.3	109.89	219.78	T cells
PBMNC		37.37	1.78	1.78	PBMNC
monocyte		34.45	9.8	19.60	monocyte
B cells PC00665	28455	31.31	60.88	121.76	B cells
dendritic cells	28441	32.25	35.19	70.38	dendritic cells
neutrophils	28440	32.3	34.36	34.36	neutrophils
eosinophils	28446	32.85	24.86	49.72	eosinophils
BM unstim		37.04	2.16	2.16	BM unstim
BM stim	treated	40	0	0.00	BM stim	−2.16
osteo dif	treated	35.82	4.39	4.39	osteo dif	1.15
osteo undif		36.06	3.81	3.81	osteo undif
chondrocytes		34.57	9.12	22.80	chondrocytes
OA Synovium IP12/01	29462	30.02	129.3	129.30	OA Synovium
OA Synovium NP10/01	29461	31.82	45.36	90.72	OA Synovium
OA Synovium NP57/00	28464	30.27	112.19	224.38	OA Synovium
RA Synovium	28466	30.84	80.36	160.72	RA Synovium
NP03/01
RA Synovium	28467	30.23	114.46	228.92	RA Synovium
NP71/00
RA Synovium	28475	36.06	3.81	7.62	RA Synovium
NP45/00
OA bone (biobank)	29217	32.73	26.65	26.65	OA bone (biobank)
OA bone Sample 1	J. Emory	31.47	55.69	111.38	OA bone
OA bone Sample 2	J. Emory	33.85	13.88	27.76	OA bone
Cartilage (pool)	Normal	40	0	0.00	Nml Cartilage (pool)
Cartilage (pool)	OA	32.49	30.73	61.46	OA Cartilage (pool)	61.46
PBL unifected	28441	34.32	10.57	21.14	PBL unifected
PBL HIV IIIB	28442	31.22	64.17	128.34	PBL HIV IIIB	6.07
MRC5 uninfected	29158	32.19	36.6	73.20	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	32.13	37.92	75.84	MRC5 HSV strain F	1.04
W12 cells	29179	32.9	24.2	48.40	W12 cells	−1.23
Keratinocytes	29180	32.54	29.85	59.70	Keratinocytes
B-actin control		27.27	643.43
genomic		26.42	1058.14
1.00E+05		19.45	100000
1.00E+05		19.73	100000
1.00E+04		22.57	10000
1.00E+04		22.68	10000
1.00E+03		25.02	1000
1.00E+03		25.15	1000
1.00E+02		28.95	100
1.00E+02		30.01	100
1.00E+01		33.54	10
1.00E+01		37.65	10
1.00E+00		38.77	1
1.00E+00		40	0
NTC		38.96	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0170] Gene Name sbg1528389ITI

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      2.55
colon tumor                      13.61
colon tumor                      10.69
colon tumor                      1.90
lung tumor                       −2.42
lung tumor                       −3.53
lung tumor                       −2.11
lung tumor                       1.26
breast tumor                     1.93
breast tumor                     3.42
breast tumor                     −2.67
breast tumor                     1.79
brain stage 5 ALZ                1.19
brain stage 5 ALZ                1.74
brain stage 5 ALZ                1.13
brain stage 5 ALZ                −1.01
lung 24 COPD                     −1.73
lung 28 COPD                     −2.66
lung 23 COPD                     −9.56
asthmatic lung                   1.20
asthmatic lung                   2.15
asthmatic lung                   5.49
asthmatic lung                   4.47
endo VEGF                        −1.72
endo bFGF                        1.25
heart (T-1) ischemic             10.08
heart (T-14) non-obstructive DCM −5.57
heart (T-3399) DCM               2.57
BM stim                          −2.16
osteo dif                        1.15
OA Cartilage (pool)              61.46
PBL HIV IIIB                     6.07
MRC5 HSV strain F                1.04
W12 cells                        −1.23

[0171] Gene Name sbg15269641SLR

[0172] Moderate overall expression. The highest normal expression is seen in the subcutaneous adipocytes, whole brain, fetal brain, liver, thymus, and fetal liver. Additional expression is seen in the colon and rectum. The highest disease expression is seen in the breast, colon, and lung normal and tumor samples as well as in the dendritic cell, the eosinophils, and the uninfected PBLS. Upregulation in 1 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in 3 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Upregulation in 4 of 4 asthmatic lung samples indicates a possible involvement in asthma. Upregulated in the stimulated bone marrow and the differentiated osteoblasts. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV. Downregulation in the W12 cells suggests that this gene may be a host factor in HPV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in the T cells, B cells, neutrophils, dendritic cells, and the eosinophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1526964ISLR	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	32.03, 31.87	86.32	94.34	90.33	3.06	16.34	1475.98
Adipocytes Zenbio
Subcutaneous Adipose	36.36, 37.6	7.63	3.8	5.72	0.96	52.36	299.21
Zenbio
Adrenal Gland	40, 40	0	0	0.00	0.61	81.97	0.00
Clontech
Whole Brain Clontech	28.56, 28.16	602.41	753.27	677.84	7.24	6.91	4681.22
Fetal Brain Clontech	34.51, 32.76	21.5	57.2	39.40	0.48	103.95	4095.11
Cerebellum Clontech	37.92, 40	3.18	0	1.59	2.17	23.04	36.64
Cervix	40, 36.56	0	6.83	3.42	2.42	20.66	70.56
Colon	34.23, 34.51	25.09	21.49	23.29	2.71	18.45	429.70
Endometrium	36.12, 36.1	8.71	8.83	8.77	0.73	68.21	598.23
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	35.01, 40	16.23	0	8.12	2.58	19.38	157.27
Jejunum	33.6, 33.32	35.76	41.84	38.80	6.60	7.58	293.94
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	33.77, 34.04	32.62	27.91	30.27	1.50	33.33	1008.83
Fetal Liver Clontech	29.78, 29.53	303.66	349.23	326.45	10.40	4.81	1569.45
Lung	36.17, 35.56	8.51	11.95	10.23	2.57	19.46	199.03
Mammary Gland	32.26, 31.59	75.95	110.01	92.98	13.00	3.85	357.62
Clontech
Myometrium	34.73, 40	19.02	0	9.51	2.34	21.37	203.21
Omentum	40, 40	0	0	0.00	3.94	12.69	0.00
Ovary	33.48, 33.38	38.36	40.55	39.46	4.34	11.52	454.55
Pancreas	40, 34.09	0	27.28	13.64	0.81	61.80	843.02
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	33, 34.94	49.96	16.95	33.46	5.48	9.12	305.25
Placenta Clontech	36, 35.49	9.33	12.42	10.88	5.26	9.51	103.37
Prostate	35.4, 40	13.09	0	6.55	3.00	16.67	109.08
Rectum	34.06, 35.27	27.61	14.02	20.82	1.23	40.65	846.14
Salivary Gland	33.4, 33.53	39.97	37.31	38.64	7.31	6.84	264.30
Clontech
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	36.6, 40	6.69	0	3.35	1.21	41.32	138.22
Small Intestine	38.85, 40	1.89	0	0.95	0.98	51.07	48.26
Clontech
Spleen	40, 34.46	0	22.14	11.07	4.92	10.16	112.50
Stomach	36.86, 35.09	5.78	15.52	10.65	2.73	18.32	195.05
Testis Clontech	40, 35.99	0	9.38	4.69	0.57	87.87	412.13
Thymus Clontech	31.03, 31.12	151.08	143.44	147.26	9.89	5.06	744.49
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	33.62, 34.06	35.48	27.61	31.55	9.71	5.15	162.44
Urinary Bladder	35.3, 36.69	13.81	6.35	10.08	5.47	9.14	92.14
Uterus	34.52, 33.14	21.39	46.31	33.85	5.34	9.36	316.95
genomic	27.07	1382.37
b-actin	27.91	866.2
1.00E+05	19.67	100000
1.00E+05	19.81	100000
1.00E+04	23.26	10000
1.00E+04	23.26	10000
1.00E+03	27.11	1000
1.00E+03	27.29	1000
1.00E+02	31.65	100
1.00E+02	32.86	100
1.00E+01	35.52	10
1.00E+01	36.09	10
1.00E+00	40	0
1.00E+00	40	1
NTC	40	0
NTC	40	0
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
sbg1526964ISLR	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	26.36	1672.61	3345.22	colon normal
colon tumor GW98-166	21940	27.67	795.52	1591.04	colon tumor	−2.10
colon normal GW98-178	22080	28.28	561.07	1122.14	colon normal
colon tumor GW98-177	22060	28.49	499.37	998.74	colon tumor	−1.12
colon normal GW98-561	23514	28.19	592.91	1185.82	colon normal
colon tumor GW98-560	23513	27.14	1073.47	2146.94	colon tumor	1.81
colon normal GW98-894	24691	27.48	884.72	1769.44	colon normal
colon tumor GW98-893	24690	27.3	984.57	1969.14	colon tumor	1.11
lung normal GW98-3	20742	27.32	970.01	1940.02	lung normal
lung tumor GW98-2	20741	28.11	618.42	1236.84	lung tumor	−1.57
lung normal GW97-179	20677	27.76	757.07	1514.14	lung normal
lung tumor GW97-178	20676	27.82	731.21	1462.42	lung tumor	−1.04
lung normal GW98-165	21922	26.55	1505.27	3010.54	lung normal
lung tumor GW98-164	21921	28.86	403.81	807.62	lung tumor	−3.73
lung normal GW98-282	22584	29.13	347.08	694.16	lung normal
lung tumor GW98-281	22583	29.38	300.54	601.08	lung tumor	−1.15
breast normal GW00-392	28750	29.57	270.38	270.38	breast normal
breast tumor GW00-391	28746	29.5	281.83	563.66	breast tumor	2.08
breast normal GW00-413	28798	28.16	602.86	602.86	breast normal
breast tumor GW00-412	28797	28.12	614.3	1228.60	breast tumor	2.04
breast normal GW00-	27592-95	30.02	208.6	208.60	breast normal
235:238
breast tumor GW00-	27588-91	29.56	271.58	271.58	breast tumor	1.30
231:234
breast normal GW98-621	23656	29.07	359.48	718.96	breast normal
breast tumor GW98-620	23655	27.48	887.97	1775.94	breast tumor	2.47
brain normal BB99-542	25507	31.18	108.39	216.78	brain normal
brain normal BB99-406	25509	32.07	65.03	130.06	brain normal
brain normal BB99-904	25546	31.93	70.43	140.86	brain normal
brain stage 5 ALZ BB99-	25502	30.08	202.54	405.08	brain stage 5 ALZ	2.49
874
brain stage 5 ALZ BB99-	25503	28.53	486.51	973.02	brain stage 5 ALZ	5.99
887
brain stage 5 ALZ BB99-	25504	29.63	261.11	522.22	brain stage 5 ALZ	3.21
862
brain stage 5 ALZ BB99-	25542	31.07	114.88	229.76	brain stage 5 ALZ	1.41
927
CT lung	normal	29.16	341.04	682.08	CT lung Nml
lung 26	normal	29.13	347.67		lung 26 Nml
lung 27	normal	34.08	20.78	20.78	lung 27 Nml
lung 24	COPD	34.17	19.77	19.77	lung 24 COPD	−12.19
lung 28	COPD	33.07	36.85	36.85	lung 28 COPD	−6.54
lung 23	COPD	36.23	6.12	6.12	lung 23 COPD	−39.37
lung 25	normal	34.16	19.9	19.90	lung 25 Nml
asthmatic lung ODO3112	29321	28.42	519.08	519.08	asthmatic lung	2.15
asthmatic lung ODO3433	29323	28.73	435.93	871.86	asthmatic lung	3.62
asthmatic lung ODO3397	29322	28.45	510.25	1020.50	asthmatic lung	4.24
asthmatic lung ODO4928	29325	28.56	480.73	961.46	asthmatic lung	3.99
endo cells	control	29.91	223.16	223.16	endo cells
endo VEGF		30.84	131.52	131.52	endo VEGF	−1.70
endo bFGF		30.69	143.15	143.15	endo bFGF	−1.56
heart Clontech	normal	30.26	182.88	365.76	heart
heart (T-1)ischemic	29417	29.44	290.65	581.30	heart (T-1)	1.59
					ischemic
heart (T-14) non-	29422	30.36	172.66	345.32	heart (T-14) non-	−1.06
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	29.37	303.23	606.46	heart (T-3399)	1.66
					DCM
adenoid GW99-269	26162	31.77	77.36	154.72	adenoid
tonsil GW98-280	22582	31.05	116.19	232.38	tonsil
T cells PC00314	28453	31.07	115.05	230.10	T cells
PBMNC		35.92	7.29	7.29	PBMNC
monocyte		36.32	5.82	11.64	monocyte
B cells PC00665	28455	29.22	330.33	660.66	B cells
dendritic cells	28441	28.3	555.32	1110.64	dendritic cells
neutrophils	28440	29.72	248.67	248.67	neutrophils
eosinophils	28446	28.45	510.81	1021.62	eosinophils
BM unstim		33.48	29.26	29.26	BM unstim
BM stim	treated	31.14	110.68	110.68	BM stim	3.78
osteo dif	treated	31.61	84.86	84.86	osteo dif	6.03
osteo undif		34.77	14.07	14.07	osteo undif
chondrocytes		29.75	244.35	610.88	chondrocytes
OA Synovium IP12/01	29462	28.4	525.46	525.46	OA Synovium
OA Synovium NP10/01	29461	30.38	170.32	340.64	OA Synovium
OA Synovium NP57/00	28464	29.66	256.61	513.22	OA Synovium
RA Synovium	28466	29.39	298.69	597.38	RA Synovium
NP03/01
RA Synovium	28467	29.23	327.25	654.50	RA Synovium
NP71/00
RA Synovium	28475	28.88	400.22	800.44	RA Synovium
NP45/00
OA bone (biobank)	29217	29.38	301.04	301.04	OA bone (biobank)
OA bone Sample 1	J. Emory	28.96	381.28	762.56	OA bone
OA bone Sample 2	J. Emory	30.39	168.95	337.90	OA bone
Cartilage (pool)	Normal	30.48	161.19	322.38	Nml Cartilage
					(pool)
Cartilage (pool)	OA	30.72	140.64	281.28	OA Cartilage	−1.15
					(pool)
PBL unifected	28441	27.63	815.72	1631.44	PBL unifected
PBL HIV IIIB	28442	29.29	315.83	631.66	PBL HIV IIIB	−2.58
MRC5 uninfected	29158	30.65	145.99	291.98	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	36.19	6.25	12.50	MRC5 HSV strain F	−23.36
W12 cells	29179	31.18	107.97	215.94	W12 cells	−5.27
Keratinocytes	29180	28.26	568.92	1137.84	Keratinocytes
B-actin control		27.63	814.59
genomic		27.4	929.42
1.00E+05		19.18	100000
1.00E+05		19.36	100000
1.00E+04		23.05	10000
1.00E+04		22.98	10000
1.00E+03		27.04	1000
1.00E+03		26.85	1000
1.00E+02		32.16	100
1.00E+02		32.19	100
1.00E+01		34.5	10
1.00E+01		40	10
1.00E+00		40	0
1.00E+00		40	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0173] Gene Name sbg15269641SLR

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −2.10
colon tumor                      −1.12
colon tumor                      1.81
colon tumor                      1.11
lung tumor                       −1.57
lung tumor                       −1.04
lung tumor                       −3.73
lung tumor                       −1.15
breast tumor                     2.08
breast tumor                     2.04
breast tumor                     1.30
breast tumor                     2.47
brain stage 5 ALZ                2.49
brain stage 5 ALZ                5.99
brain stage 5 ALZ                3.21
brain stage 5 ALZ                1.41
lung 24 COPD                     −12.19
lung 28 COPD                     −6.54
lung 23 COPD                     −39.37
asthmatic lung                   2.15
asthmatic lung                   3.62
asthmatic lung                   4.24
asthmatic lung                   3.99
endo VEGF                        −1.70
endo bFGF                        −1.56
heart (T-1) ischemic             1.59
heart (T-14) non-obstructive DCM −1.06
heart (T-3399) DCM               1.66
BM stim                          3.78
osteo dif                        6.03
OA Cartilage (pool)              −1.15
PBL HIV IIIB                     −2.58
MRC5 HSV strain F                −23.36
W12 cells                        −5.27

[0174] Gene Name sbg1539439TSPK

[0175] Moderate to low overall expression. The highest normal expression is seen in the whole brain, testis, endometrium, and trachea. The highest disease expression is seen in one of the lung tumor samples, one of the breast tumor samples, and one of the normal breast samples. Upregulation in 1 of 4 colon tumor samples, 1 of 4 lung tumor samples, and 1 of 4 breast tumor samples implicates this gene in cancers of the colon, lung, and breast. Upregulation in 1 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Downregulation in the VEGF and bFGF-treated endothelial cells suggests an involvement in angiogenesis. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as obstructive DCM and ischemia Downregulated in the differentiated osteoblasts. Downregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1539439TSPK	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 36.02	0	4.84	2.42	3.06	16.34	39.54
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	36.66, 40	3.29	0	1.65	0.61	81.97	134.84
Whole Brain Clontech	30.21, 30.36	157.24	143.95	150.60	7.24	6.91	1040.02
Fetal Brain Clontech	36.07, 40	4.69	0	2.35	0.48	103.95	243.76
Cerebellum Clontech	40, 40	0	0	0.00	2.17	23.04	0.00
Cervix	40, 40	0	0	0.00	2.42	20.66	0.00
Colon	35.92, 35.85	5.15	5.36	5.26	2.71	18.45	96.96
Endometrium	35.96, 35.78	5.03	5.59	5.31	0.73	68.21	362.21
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	40, 40	0	0	0.00	2.58	19.38	0.00
Jejunum	34.85, 33.91	9.76	17.17	13.47	6.60	7.58	102.01
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	40, 40	0	0	0.00	1.50	33.33	0.00
Fetal Liver Clontech	33.25, 33.41	25.49	23.06	24.28	10.40	4.81	116.71
Lung	40, 33.18	0	26.53	13.27	2.57	19.46	258.07
Mammary Gland	34.09, 32.82	15.36	32.98	24.17	13.00	3.85	92.96
Clontech
Myometrium	40, 37.47	0	2.02	1.01	2.34	21.37	21.58
Omentum	40, 40	0	0	0.00	3.94	12.69	0.00
Ovary	35.55, 34.85	6.4	9.78	8.09	4.34	11.52	93.20
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	34.15, 34.78	14.8	10.18	12.49	5.48	9.12	113.96
Placenta Clontech	40, 36.41	0	3.84	1.92	5.26	9.51	18.25
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland Clontech	40, 35.17	0	8.04	4.02	7.31	6.84	27.50
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	40, 35.97	0	4.99	2.50	4.92	10.16	25.36
Stomach	40, 35.78	0	5.59	2.80	2.73	18.32	51.19
Testis Clontech	40, 33.83	0	17.92	8.96	0.57	87.87	787.35
Thymus Clontech	33.81, 32.94	18.15	30.59	24.37	9.89	5.06	123.21
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	32.09, 31.16	50.98	89.2	70.09	9.71	5.15	360.92
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	35.73, 34.51	5.74	11.97	8.86	5.34	9.36	82.91
genomic	27.01	1071.32
b-actin	27.61	745.98
1.00E+05	19.6	100000
1.00E+05	19.43	100000
1.00E+04	23.05	10000
1.00E+04	23.03	10000
1.00E+03	26.8	1000
1.00E+03	26.78	1000
1.00E+02	31.67	100
1.00E+02	32.36	100
1.00E+01	33.69	10
1.00E+01	40	0
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1539439TSPK	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	33.02	44.92	89.84	colon normal
colon tumor GW98-166	21940	30.69	149.66	299.32	colon tumor	3.33
colon normal GW98-178	22080	40	0	0.00	colon normal
colon tumor GW98-177	22060	32.11	71.99	143.98	colon tumor	143.98
colon normal GW98-561	23514	33.18	41.4	82.80	colon normal
colon tumor GW98-560	23513	33.52	34.64	69.28	colon tumor	−1.20
colon normal GW98-894	24691	33.58	33.62	67.24	colon normal
colon tumor GW98-893	24690	31.29	109.68	219.36	colon tumor	3.26
lung normal GW98-3	20742	31.18	116.02	232.04	lung normal
lung tumor GW98-2	20741	31.65	90.98	181.96	lung tumor	−1.28
lung normal GW97-179	20677	31.85	82.35	164.70	lung normal
lung tumor GW97-178	20676	31.88	81.04	162.08	lung tumor	−1.02
lung normal GW98-165	21922	30.93	132.27	264.54	lung normal
lung tumor GW98-164	21921	27.53	762.99	1525.98	lung tumor	5.77
lung normal GW98-282	22584	29.58	265.31	530.62	lung normal
lung tumor GW98-281	22583	30.27	185.74	371.48	lung tumor	−1.43
breast normal GW00-392	28750	29.47	281.23	281.23	breast normal
breast tumor GW00-391	28746	28.91	374.08	748.16	breast tumor	2.66
breast normal GW00-413	28798	31.18	115.93	115.93	breast normal
breast tumor GW00-412	28797	28.24	530.48	1060.96	breast tumor	9.15
breast normal GW00-	27592-95	31.64	91.39	91.39	breast normal
235:238
breast tumor GW00-	27588-91	29.12	335.78	335.78	breast tumor	3.67
231:234
breast normal GW98-621	23656	28.12	564.44	1128.88	breast normal
breast tumor GW98-620	23655	32.45	60.32	120.64	breast tumor	−9.36
brain normal BB99-542	25507	31.13	119.34	238.68	brain normal
brain normal BB99-406	25509	33.22	40.62	81.24	brain normal
brain normal BB99-904	25546	32.19	69.14	138.28	brain normal
brain stage 5 ALZ BB99-	25502	31.35	106.38	212.76	brain stage 5 ALZ	1.39
874
brain stage 5 ALZ BB99-	25503	29.76	241.33	482.66	brain stage 5 ALZ	3.16
887
brain stage 5 ALZ BB99-	25504	31.08	122.52	245.04	brain stage 5 ALZ	1.60
862
brain stage 5 ALZ BB99-	25542	31.16	117.27	234.54	brain stage 5 ALZ	1.54
927
CT lung	normal	32.4	61.98	123.96	CT lung Nml
lung 26	normal	29.22	319.73		lung 26 Nml
lung 27	normal	35.13	15.13	15.13	lung 27 Nml
lung 24	COPD	40	0	0.00	lung 24 COPD	−46.36
lung 28	COPD	40	0	0.00	lung 28 COPD	−46.36
lung 23	COPD	40	0	0.00	lung 23 COPD	−46.36
lung 25	normal	40	0	0.00	lung 25 Nml
asthmatic lung ODO3112	29321	33.11	42.85	42.85	asthmatic lung	−1.08
asthmatic lung ODO3433	29323	32.67	53.81	107.62	asthmatic lung	2.32
asthmatic lung ODO3397	29322	32.05	74.04	148.08	asthmatic lung	3.19
asthmatic lung ODO4928	29325	32.44	60.75	121.50	asthmatic lung	2.62
endo cells	control	34.69	18.99	18.99	endo cells
endo VEGF		40	0	0.00	endo VEGF	−18.99
endo bFGF		40	0	0.00	endo bFGF	−18.99
heart Clontech	normal	33.48	35.46	70.92	heart
heart (T-1) ischemic	29417	31.11	120.31	240.62	heart (T-1) ischemic	3.39
heart (T-14) non-	29422	33.38	37.23	74.46	heart (T-14) non-	1.05
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	31.22	113.98	227.96	heart (T-3399) DCM	3.21
adenoid GW99-269	26162	34.45	21.49	42.98	adenoid
tonsil GW98-280	22582	32.46	60.05	120.10	tonsil
T cells PC00314	28453	37.71	3.98	7.96	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		40	0	0.00	monocyte
B cells PC00665	28455	40	0	0.00	B cells
dendritic cells	28441	40	0	0.00	dendritic cells
neutrophils	28440	40	0	0.00	neutrophils
eosinophils	28446	40	0	0.00	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	0.00
osteo dif	treated	40	0	0.00	osteo dif	−9.66
osteo undif		36	9.66	9.66	osteo undif
chondrocytes		35.21	14.53	36.33	chondrocytes
OA Synovium IP12/01	29462	29.83	232.79	232.79	OA Synovium
OA Synovium NP10/01	29461	34.09	25.81	51.62	OA Synovium
OA Synovium NP57/00	28464	30.77	143.53	287.06	OA Synovium
RA Synovium	28466	32.4	61.91	123.82	RA Synovium
NP03/01
RA Synovium	28467	30.44	170.17	340.34	RA Synovium
NP71/00
RA Synovium	28475	31.75	86.5	173.00	RA Synovium
NP45/00
OA bone (biobank)	29217	33.41	36.7	36.70	OA bone (biobank)
OA bone Sample 1	J. Emory	31.82	83.64	167.28	OA bone
OA bone Sample 2	J. Emory	33.54	34.41	68.82	OA bone
Cartilage (pool)	Normal	31.35	106.16	212.32	Nml Cartilage (pool)
Cartilage (pool)	OA	34.25	23.76	47.52	OA Cartilage (pool)	−4.47
PBL unifected	28441	34.16	24.9	49.80	PBL unifected
PBL HIV IIIB	28442	35.26	14.17	28.34	PBL HIV IIIB	−1.76
MRC5 uninfected	29158	30.16	196.24	392.48	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	32.39	62.07	124.14	MRC5 HSV strain F	−3.16
W12 cells	29179	29.09	342.35	684.70	W12 cells	1.41
Keratinocytes	29180	29.76	242.11	484.22	Keratinocytes
B-actin control		27.19	912.41
genomic		26.06	1629.6
1.00E+05		18.96	100000
1.00E+05		19	100000
1.00E+04		22.27	10000
1.00E+04		22.05	10000
1.00E+03		25.89	1000
1.00E+03		26.06	1000
1.00E+02		31.08	100
1.00E+02		31.14	100
1.00E+01		40	0
1.00E+01		37.69	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0176] Gene Name sbg1539439TSPK

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      3.33
colon tumor                      143.98
colon tumor                      −1.20
colon tumor                      3.26
lung tumor                       −1.28
lung tumor                       −1.02
lung tumor                       5.77
lung tumor                       −1.43
breast tumor                     2.66
breast tumor                     9.15
breast tumor                     3.67
breast tumor                     −9.36
brain stage 5 ALZ                1.39
brain stage 5 ALZ                3.16
brain stage 5 ALZ                1.60
brain stage 5 ALZ                1.54
lung 24 COPD                     −46.36
lung 28 COPD                     −46.36
lung 23 COPD                     −46.36
asthmatic lung                   −1.08
asthmatic lung                   2.32
asthmatic lung                   3.19
asthmatic lung                   2.62
endo VEGF                        −18.99
endo bFGF                        −18.99
heart (T-1) ischemic             3.39
heart (T-14) non-obstructive DCM 1.05
heart (T-3399) DCM               3.21
BM stim                          0.00
osteo dif                        −9.66
OA Cartilage (pool)              −4.47
PBL HIV IIIB                     −1.76
MRC5 HSV strain F                −3.16
W12 cells                        1.41

[0177] Gene Name sbg1571349lipase

[0178] Highly specific overall expression. The highest normal expression is seen in the whole brain, colon, and rectum with additional high expression in other GI tract-specific tissues including the ileum and the jejunum. This pattern of expression suggests that this gene may be involved in diseases of the digestive tract such as IBS. The highest disease expression is seen in the colon normal and tumor samples. Large upregulation in 1 of 4 colon tumor samples is sufficient to make a claim for colon cancer. Upregulation in 1 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia Upregulation in the HIV-infected PBL cells suggests that this gene may be a host factor in HIV. Upregulation in the W12 cells suggests that this gene may be a host factor in HPV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1571349-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
lipase	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	40, 40	0	0	0.00	3.06	16.34	0.00
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	28.65, 28.66	381.49	377.43	379.46	7.24	6.91	2620.58
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	40, 40	0	0	0.00	2.17	23.04	0.00
Cervix	40, 40	0	0	0.00	2.42	20.66	0.00
Colon	29.1, 28.56	289.29	402.29	345.79	2.71	18.45	6379.89
Endometrium	40, 40	0	0	0.00	0.73	68.21	0.00
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	33.37, 34.4	21.83	11.72	16.78	2.58	19.38	325.10
Jejunum	33.03, 33.89	26.82	15.94	21.38	6.60	7.58	161.97
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	36.4, 35.75	3.49	5.18	4.34	1.50	33.33	144.50
Fetal Liver Clontech	33.02	27.08	82.43	54.76	10.40	4.81	263.25
Lung	40, 40	0	0.68	0.34	2.57	19.46	6.61
Mammary Gland	38.18, 40	1.19	0	0.60	13.00	3.85	2.29
Clontech
Myometrium	40, 40	0	0	0.00	2.34	21.37	0.00
Omentum	40, 40	0	0	0.00	3.94	12.69	0.00
Ovary	34.85, 34.22	8.94	13.1	11.02	4.34	11.52	126.96
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	38.47, 35.13	1	7.55	4.28	5.48	9.12	39.01
Placenta Clontech	40, 40	0	0	0.00	5.26	9.51	0.00
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	30.07, 30.1	161.52	157.91	159.72	1.23	40.65	6492.48
Salivary Gland Clontech	32.8, 32.31	30.88	41.68	36.28	7.31	6.84	248.15
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	40, 40	0	0	0.00	4.92	10.16	0.00
Stomach	40, 40	0	0	0.00	2.73	18.32	0.00
Testis Clontech	40, 35.34	0	6.65	3.33	0.57	87.87	292.18
Thymus Clontech	32.58, 33.71	35.33	17.82	26.58	9.89	5.06	134.35
Thyroid	40, 35.83	0	4.93	2.47	2.77	18.05	44.49
Trachea Clontech	37.09, 40	2.3	0	1.15	9.71	5.15	5.92
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	34.81, 34.22	9.16	13.06	11.11	5.34	9.36	104.03
genomic	26.45	1442.6
b-actin	27.74	660.76
1.00E+05	19.65	100000
1.00E+05	19.7	100000
1.00E+04	23.16	10000
1.00E+04	23.05	10000
1.00E+03	26.21	1000
1.00E+03	26.74	1000
1.00E+02	30.93	100
1.00E+02	32.11	100
1.00E+01	33.74	10
1.00E+01	35.25	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1571349-	(GSK		GOI	total		Disease
lipase	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	21.81	18527.55	37055.10	colon normal
colon tumor GW98-166	21940	24.48	3977.68	7955.36	colon tumor	−4.66
colon normal GW98-178	22080	30.22	144.61	289.22	colon normal
colon tumor GW98-177	22060	25.35	2406.82	4813.64	colon tumor	16.64
colon normal GW98-561	23514	22.94	9655.42	19310.84	colon normal
colon tumor GW98-560	23513	22.94	9655.62	19311.24	colon tumor	1.00
colon normal GW98-894	24691	21.03	29052.3	58104.60	colon normal
colon tumor GW98-893	24690	23.12	8692.86	17385.72	colon tumor	−3.34
lung normal GW98-3	20742	32.33	43.02	86.04	lung normal
lung tumor GW98-2	20741	40	0	0.00	lung tumor	−86.04
lung normal GW97-179	20677	29.92	172.76	345.52	lung normal
lung tumor GW97-178	20676	31.38	74.23	148.46	lung tumor	−2.33
lung normal GW98-165	21922	31.1	87.26	174.52	lung normal
lung tumor GW98-164	21921	31.13	85.73	171.46	lung tumor	−1.02
lung normal GW98-282	22584	33.29	24.7	49.40	lung normal
lung tumor GW98-281	22583	33.61	20.52	41.04	lung tumor	−1.20
breast normal GW00-	28750	33.03	28.62	28.62	breast normal
392
breast tumor GW00-391	28746	32.43	40.5	81.00	breast tumor	2.83
breast normal GW00-	28798	34.93	9.6	9.60	breast normal
413
breast tumor GW00-412	28797	33.16	26.53	53.06	breast tumor	5.53
breast normal GW00-	27592-95	34.31	13.71	13.71	breast normal
235:238
breast tumor GWOO-	27588-91	32.53	38.16	38.16	breast tumor	2.78
231:234
breast normal GW98-	23656	32.24	45.24	90.48	breast normal
621
breast tumor GW98-620	23655	31.55	67.35	134.70	breast tumor	1.49
brain normal BB99-542	25507	31.2	82.43	164.86	brain normal
brain normal BB99-406	25509	30.13	152.5	305.00	brain normal
brain normal BB99-904	25546	33.42	22.88	45.76	brain normal
brain stage 5 ALZ	25502	31.76	59.61	119.22	brain stage 5 ALZ	−1.44
BB99-874
brain stage 5 ALZ	25503	30.05	159.48	318.96	brain stage 5 ALZ	1.86
BB99-887
brain stage 5 ALZ	25504	30.83	102.18	204.36	brain stage 5 ALZ	1.19
BB99-862
brain stage 5 ALZ	25542	30.06	159.13	318.26	brain stage 5 ALZ	1.85
BB99-927
CT lung	normal	34.2	14.61	29.22	CT lung Nml
lung 26	normal	30.25	142.31		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	40	0	0.00	lung 24 COPD	−9.74
lung 28	COPD	40	0	0.00	lung 28 COPD	−9.74
lung 23	COPD	40	0	0.00	lung 23 COPD	−9.74
lung 25	normal	40	0	0.00	lung 25 Nml
asthmatic lung	29321	36.18	4.67	4.67	asthmatic lung	−2.09
ODO3112
asthmatic lung	29323	34.06	15.81	31.62	asthmatic lung	3.25
ODO3433
asthmatic lung	29322	32.15	47.6	95.20	asthmatic lung	9.77
ODO3397
asthmatic lung	29325	35.92	5.42	10.84	asthmatic lung	1.11
ODO4928
endo cells	control	40	0	0.00	endo cells
endo VEGF		40	0	0.00	endo VEGF	0.00
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	32.63	36.17	72.34	heart (T-1) ischemic	72.34
heart (T-14) non-	29422	32.06	50.14	100.28	heart (T-14) non-	100.28
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	33.12	27.25	54.50	heart (T-3399) DCM	54.50
adenoid GW99-269	26162	34.14	15.07	30.14	adenoid
tonsil GW98-280	22582	32.93	30.43	60.86	tonsil
T cells PC00314	28453	34.46	12.57	25.14	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		40	0	0.00	monocyte
B cells PC00665	28455	32.13	48.2	96.40	B cells
dendritic cells	28441	40	0	0.00	dendritic cells
neutrophils	28440	36.83	3.21	3.21	neutrophils
eosinophils	28446	40	0	0.00	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	0.00
osteo dif	treated	40	0	0.00	osteo dif	0.00
osteo undif		40	0	0.00	osteo undif
chondrocytes		31.82	57.77	144.43	chondrocytes
OA Synovium IP12/01	29462	31.1	87.17	87.17	OA Synovium
OA Synovium NP10/01	29461	31.99	52.32	104.64	OA Synovium
OA Synovium NP57/00	28464	30.73	107.79	215.58	OA Synovium
RA Synovium	28466	32.67	35.27	70.54	RA Synovium
NP03/01
RA Synovium	28467	30.36	134.08	268.16	RA Synovium
NP71/00
RA Synovium	28475	32.2	46.39	92.78	RA Synovium
NP45/00
OA bone (biobank)	29217	34.27	14.02	14.02	OA bone (biobank)
OA bone Sample 1	J. Emory	32.28	44.28	88.56	OA bone
OA bone Sample 2	J. Emory	32.12	48.37	96.74	OA bone
Cartilage (pool)	Normal	29.16	267.1	534.20	Nml Cartilage (pool)
Cartilage (pool)	OA	32.62	36.38	72.76	OA Cartilage (pool)	−7.34
PBL unifected	28441	40	0	0.00	PBL unifected
PBL HIV IIIB	28442	34.18	14.74	29.48	PBL HIV IIIB	29.48
MRC5 uninfected	29158	32.13	48.26	96.52	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	31.75	60.03	120.06	MRC5 HSV strain F	1.24
W12 cells	29179	32.75	33.72	67.44	W12 cells	67.44
Keratinocytes	29180	40	0	0.00	Keratinocytes
B-actin control		27.39	743.52
genomic		26.45	1273.58
1.00E+05		19.22	100000
1.00E+05		19.32	100000
1.00E+04		22.57	10000
1.00E+04		22.44	10000
1.00E+03		26.39	1000
1.00E+03		26.17	1000
1.00E+02		31.23	100
1.00E+02		32.05	100
1.00E+01		33.62	10
1.00E+01		35.71	10
1.00E+00		40	0
1.00E+00		40	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0179] Gene Name sbg1571349lipase

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −4.66
colon tumor                      16.64
colon tumor                      1.00
colon tumor                      −3.34
lung tumor                       −86.04
lung tumor                       −2.33
lung tumor                       −1.02
lung tumor                       −1.20
breast tumor                     2.83
breast tumor                     5.53
breast tumor                     2.78
breast tumor                     1.49
brain stage 5 ALZ                −1.44
brain stage 5 ALZ                1.86
brain stage 5 ALZ                1.19
brain stage 5 ALZ                1.85
lung 24 COPD                     −9.74
lung 28 COPD                     −9.74
lung 23 COPD                     −9.74
asthmatic lung                   −2.09
asthmatic lung                   3.25
asthmatic lung                   9.77
asthmatic lung                   1.11
endo VEGF                        0.00
endo bFGF                        0.00
heart (T-1) ischemic             72.34
heart (T-14) non-obstructive DCM 100.28
heart (T-3399) DCM               54.50
BM stim                          0.00
osteo dif                        0.00
OA Cartilage (pool)              −7.34
PBL HIV IIIB                     29.48
MRC5 HSV strain F                1.24
W12 cells                        67.44

[0180] Gene Name sbg1565539aCONNEXIN

[0181] Failed

[0182] Gene Name sbg1565539bCONNEXIN

[0183] Highly specific overall expression. The highest normal expression is seen primarily in tissues representing the female reproductive system including the endometrium, ovary, placenta, and uterus as well as tissues representing the GI tract such as the colon, ileum, jejunum, omentum, and stomach. Additional high expression is seen in the whole brain and the liver. Highest disease expression is specific to the Alzheimer's brain samples. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as obstructive DCM and ischemia. Upregulated in the differentiated osteoblasts. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in the T cells, B cells, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis. Upregulation in the W12 cells suggests that this gene may be a host factor in HPV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1565539b-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
CONNEXIN	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous Adipocytes	36.28, 40	5.2	0	2.60	3.06	16.34	42.48
Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	28.42, 28.6	530.94	479.68	505.31	7.24	6.91	3489.71
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	40, 39.91	0	4.34	2.17	2.17	23.04	50.00
Cervix	40, 40	0	0	0.00	2.42	20.66	0.00
Colon	40, 37.11	0	3.19	1.60	2.71	18.45	29.43
Endometrium	40, 36.59	0	4.34	2.17	0.73	68.21	148.02
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	40, 35.95	0	6.3	3.15	2.58	19.38	61.05
Jejunum	35.01, 35.1	11	10.42	10.71	6.60	7.58	81.14
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	35.14, 35.33	10.15	9.1	9.63	1.50	33.33	320.83
Fetal Liver Clontech	35.29, 34.74	9.33	12.9	11.12	10.40	4.81	53.44
Lung	40, 40	0	0	0.00	2.57	19.46	0.00
Mammary Gland	40, 36.16	0	5.58	2.79	13.00	3.85	10.73
Clontech
Myometrium	40, 40	0	0	0.00	2.34	21.37	0.00
Omentum	35.17, 33.81	9.98	22.2	16.09	3.94	12.69	204.19
Ovary	40, 34.09	0	18.92	9.46	4.34	11.52	108.99
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	35.74, 35.13	7.14	10.21	8.68	5.48	9.12	79.15
Placenta Clontech	34.95, 35.76	11.39	7.07	9.23	5.26	9.51	87.74
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland Clontech	40, 36.02	0	6.04	3.02	7.31	6.84	20.66
Skeletal Muscle Clontech	40, 40	0	0	0.00	1.26	39.68	0.00
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	35.14, 40	10.2	0	5.10	4.92	10.16	51.83
Stomach	36.06, 35.41	5.92	8.67	7.30	2.73	18.32	133.61
Testis Clontech	40, 40	0	0	0.00	0.57	87.87	0.00
Thymus Clontech	35.49, 40	8.25	0	4.13	9.89	5.06	20.85
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	40, 40	0	0	0.00	9.71	5.15	0.00
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	35.46, 34.1	8.44	18.73	13.59	5.34	9.36	127.20
genomic	27.32	1018.5
b-actin	28.07	654.85
1.00E+05	19.58	100000
1.00E+05	19.75	100000
1.00E+04	23.28	10000
1.00E+04	23.36	10000
1.00E+03	27.08	1000
1.00E+03	27.26	1000
1.00E+02	31.3	100
1.00E+02	31.53	100
1.00E+01	40	10
1.00E+01	40	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1565539b-	(GSK		GOI	total		Disease
CONNEXIN	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	34.17	36.97	73.94	colon normal
colon tumor GW98-166	21940	34.12	38.19	76.38	colon tumor	1.03
colon normal GW98-178	22080	36.65	9.19	18.38	colon normal
colon tumor GW98-177	22060	34.02	40.31	80.62	colon tumor	4.39
colon normal GW98-561	23514	36.43	10.4	20.80	colon normal
colon tumor GW98-560	23513	35.4	18.5	37.00	colon tumor	1.78
colon normal GW98-894	24691	33.79	46	92.00	colon normal
colon tumor GW98-893	24690	35.1	21.92	43.84	colon tumor	−2.10
lung normal GW98-3	20742	33.16	65.36	130.72	lung normal
lung tumor GW98-2	20741	40	0	0.00	lung tumor	−130.72
lung normal GW97-179	20677	33.44	55.88	111.76	lung normal
lung tumor GW97-178	20676	33.44	55.89	111.78	lung tumor	1.00
lung normal GW98-165	21922	32.96	73.02	146.04	lung normal
lung tumor GW98-164	21921	32.32	104.66	209.32	lung tumor	1.43
lung normal GW98-282	22584	40	0	0.00	lung normal
lung tumor GW98-281	22583	34.38	32.96	65.92	lung tumor	65.92
breast normal GW00-392	28750	33.59	51.32	51.32	breast normal
breast tumor GW00-391	28746	33.66	49.38	98.76	breast tumor	1.92
breast normal GW00-413	28798	33.6	51.05	51.05	breast normal
breast tumor GW00-412	28797	34.67	28.04	56.08	breast tumor	1.10
breast normal GW00-	27592-95	33.2	63.96	63.96	breast normal
235:238
breast tumor GW00-	27588-91	33.09	68.07	68.07	breast tumor	1.06
231:234
breast normal GW98-621	23656	33.23	62.78	125.56	breast normal
breast tumor GW98-620	23655	33.89	43.44	86.88	breast tumor	−1.45
brain normal BB99-542	25507	29.44	528.77	1057.54	brain normal
brain normal BB99-406	25509	32.03	123.74	247.48	brain normal
brain normal BB99-904	25546	29	679.06	1358.12	brain normal
brain stage 5 ALZ BB99-	25502	32.89	76.09	152.18	brain stage 5 ALZ	−5.83
874
brain stage 5 ALZ BB99-	25503	30.24	337.73	675.46	brain stage 5 ALZ	−1.31
887
brain stage 5 ALZ BB99-	25504	30.43	303.67	607.34	brain stage 5 ALZ	−1.46
862
brain stage 5 ALZ BB99-	25542	29.62	478.76	957.52	brain stage 5 ALZ	1.08
927
CT lung	normal	40	0	0.00	CT lung Nml
lung 26	normal	40	1015.6		lung 26 Nml
lung 27	normal	36.73	8.79	8.79	lung 27 Nml
lung 24	COPD	40	0	0.00	lung 24 COPD	−9.84
lung 28	COPD	40	0	0.00	lung 28 COPD	−9.84
lung 23	COPD	35.64	16.25	16.25	lung 23 COPD	1.65
lung 25	normal	35.2	20.72	20.72	lung 25 Nml
asthmatic lung ODO3112	29321	35.75	15.26	15.26	asthmatic lung	1.55
asthmatic lung ODO3433	29323	34.63	28.66	57.32	asthmatic lung	5.83
asthmatic lung ODO3397	29322	36.61	9.41	18.82	asthmatic lung	1.91
asthmatic lung ODO4928	29325	34.2	36.36	72.72	asthmatic lung	7.39
endo cells	control	40	0	0.00	endo cells
endo VEGF		40	0	0.00	endo VEGF	0.00
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	35.4	18.58	37.16	heart (T-1)	37.16
					ischemic
heart (T-14) non-	29422	40	0	0.00	heart (T-14) non-	0.00
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	35.3	19.59	39.18	heart (T-3399) DCM	39.18
adenoid GW99-269	26162	40	0	0.00	adenoid
tonsil GW98-280	22582	40	0	0.00	tonsil
T cells PC00314	28453	31.96	128.72	257.44	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		36.29	11.27	22.54	monocyte
B cells PC00665	28455	33.69	48.6	97.20	B cells
dendritic cells	28441	40	0	0.00	dendritic cells
neutrophils	28440	32.43	98.82	98.82	neutrophils
eosinophils	28446	34.77	26.38	52.76	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	0.00
osteo dif	treated	35.04	22.76	22.76	osteo dif	22.76
osteo undif		40	0	0.00	osteo undif
chondrocytes		32.27	107.58	268.95	chondrocytes
OA Synovium IP12/01	29462	32.64	87.38	87.38	OA Synovium
OA Synovium NP10/01	29461	35.16	21.21	42.42	OA Synovium
OA Synovium NP57/00	28464	32.72	83.67	167.34	OA Synovium
RA Synovium NP03/01	28466	33.11	67.32	134.64	RA Synovium
RA Synovium	28467	34.14	37.67	75.34	RA Synovium
NP71/00
RA Synovium	28475	33.02	70.9	141.80	RA Synovium
NP45/00
OA bone (biobank)	29217	34.82	25.76	25.76	OA bone (biobank)
OA bone Sample 1	J. Emory	31.76	143.77	287.54	OA bone
OA bone Sample 2	J. Emory	33.25	62.21	124.42	OA bone
Cartilage (pool)	Normal	32.24	109.9	219.80	Nml Cartilage (pool)
Cartilage (pool)	OA	33.41	56.7	113.40	OA Cartilage (pool)	−1.94
PBL unifected	28441	33.37	58.1	116.20	PBL unifected
PBL HIV IIIB	28442	34.85	25.34	50.68	PBL HIV IIIB	−2.29
MRC5 uninfected	29158	32.53	93.06	186.12	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	34.4	32.5	65.00	MRC5 HSV strain F	−2.86
W12 cells	29179	40	1.27	2.54	W12 cells	2.54
Keratinocytes	29180	40	0	0.00	Keratinocytes
B-actin control		27.94	1228.38
genomic		27.61	1485.33
1.00E+05		20.29	100000
1.00E+05		20.32	100000
1.00E+04		24.09	10000
1.00E+04		24.12	10000
1.00E+03		28.12	1000
1.00E+03		28.02	1000
1.00E+02		32.15	100
1.00E+02		32.83	100
1.00E+01		37.6	10
1.00E+01		35.56	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0184] Gene Name sbg1565539bCONNEXIN

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.03
colon tumor                      4.39
colon tumor                      1.78
colon tumor                      −2.10
lung tumor                       −130.72
lung tumor                       1.00
lung tumor                       1.43
lung tumor                       65.92
breast tumor                     1.92
breast tumor                     1.10
breast tumor                     1.06
breast tumor                     −1.45
brain stage 5 ALZ                −5.83
brain stage 5 ALZ                −1.31
brain stage 5 ALZ                −1.46
brain stage 5 ALZ                1.08
lung 24 COPD                     −9.84
lung 28 COPD                     −9.84
lung 23 COPD                     1.65
asthmatic lung                   1.55
asthmatic lung                   5.83
asthmatic lung                   1.91
asthmatic lung                   7.39
endo VEGF                        0.00
endo bFGF                        0.00
heart (T-1) ischemic             37.16
heart (T-14) non-obstructive DCM 0.00
heart (T-3399) DCM               39.18
BM stim                          0.00
osteo dif                        22.76
OA Cartilage (pool)              −1.94
PBL HIV IIIB                     −2.29
MRC5 HSV strain F                −2.86
W12 cells                        2.54

[0185] Gene Name sbg1563294acadherin

[0186] Moderate to low overall expression. The highest normal expression is seen in the whole brain, prostate, thymus, and trachea. The highest disease expression is seen in some of the colon and lung normal and tumor samples as well as in 2 of the asthmatic lung samples. Upregulation in 1 of 4 colon tumor samples and 1 of 4 lung tumor samples implicates this gene in cancers of the colon and lung. Upregulation in 3 of 4 asthmatic lung samples coupled with immune cell expression in indicates a possible involvement in asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. This gene appears to show the same expression patterns in the disease samples as 1563294b. Based on this, it is likely that these two different primer sets are amplifying the same gene.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1563294a-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
cadherin	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	35.18, 34.05	10.16	20.47	15.32	3.06	16.34	250.25
Adipocytes Zenbio
Subcutaneous	40, 37.07	0	3.12	1.56	0.96	52.36	81.68
Adipose Zenbio
Adrenal Gland	36.83, 37.11	3.63	3.04	3.34	0.61	81.97	273.36
Clontech
Whole Brain Clontech	28.05, 27.84	855.18	975.02	915.10	7.24	6.91	6319.75
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	36.55, 36.15	4.33	5.53	4.93	2.17	23.04	113.59
Cervix	40, 37.37	0	2.59	1.30	2.42	20.66	26.76
Colon	36.27, 35.14	5.13	10.4	7.77	2.71	18.45	143.27
Endometrium	40, 40	0	0	0.00	0.73	68.21	0.00
Esophagus	36.74, 40	3.84	0	1.92	1.37	36.50	70.07
Heart Clontech	40, 37.03	0	3.2	1.60	1.32	37.88	60.61
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	40, 40	0	0	0.00	2.58	19.38	0.00
Jejunum	34.97, 33.38	11.53	31.09	21.31	6.60	7.58	161.44
Kidney	40, 35.03	0	11.1	5.55	2.12	23.58	130.90
Liver	40, 36.73	0	3.86	1.93	1.50	33.33	64.33
Fetal Liver Clontech	32.3, 32.52	60.85	53.09	56.97	10.40	4.81	273.89
Lung	33.6, 34.06	27.01	20.39	23.70	2.57	19.46	461.09
Mammary Gland	32.92, 32.24	41.47	63.25	52.36	13.00	3.85	201.38
Clontech
Myometrium	40, 39.48	0	0.7	0.35	2.34	21.37	7.48
Omentum	34.65, 34.7	14.12	13.68	13.90	3.94	12.69	176.40
Ovary	35.24, 37.08	9.75	3.11	6.43	4.34	11.52	74.08
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	35.56, 36.73	7.98	3.87	5.93	5.48	9.12	54.06
Placenta Clontech	34.66, 36.1	13.99	5.72	9.86	5.26	9.51	93.68
Prostate	32.65, 33.31	48.83	32.38	40.61	3.00	16.67	676.75
Rectum	36.01, 40	6.03	0	3.02	1.23	40.65	122.56
Salivary Gland	36.61, 35.77	4.15	7	5.58	7.31	6.84	38.13
Clontech
Skeletal Muscle	40, 37.84	0	1.93	0.97	1.26	39.68	38.29
Clontech
Skin	40, 38.04	0	1.71	0.86	1.21	41.32	35.33
Small Intestine	36.86, 40	3.56	0	1.78	0.98	51.07	90.91
Clontech
Spleen	33.78, 34.67	24.18	13.89	19.04	4.92	10.16	193.45
Stomach	40, 35.96	0.37	6.22	3.30	2.73	18.32	60.35
Testis Clontech	40, 36.66	0	4.04	2.02	0.57	87.87	177.50
Thymus Clontech	30, 29.53	254.19	341.24	297.72	9.89	5.06	1505.13
Thyroid	36.28, 35.31	5.1	9.34	7.22	2.77	18.05	130.32
Trachea Clontech	31.45, 30.55	103.03	180.44	141.74	9.71	5.15	729.84
Urinary Bladder	35.12, 34.3	10.54	17.5	14.02	5.47	9.14	128.15
Uterus	33.99, 34.37	21.21	16.81	19.01	5.34	9.36	178.00
genomic	27.38	1297.04
b-actin	28.45	666.41
1.00E+05	20.54	100000
1.00E+05	20.49	100000
1.00E+04	23.66	10000
1.00E+04	24.02	10000
1.00E+03	27.83	1000
1.00E+03	27.45	1000
1.00E+02	32.08	100
1.00E+02	32.23	100
1.00E+01	33.15	10
1.00E+01	36.57	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1563294a-	(GSK		GOI	total		Disease
cadherin	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	30.45	272.65	545.30	colon normal
colon tumor GW98-166	21940	29.54	466.79	933.58	colon tumor	1.71
colon normal GW98-178	22080	32.11	102.37	204.74	colon normal
colon tumor GW98-177	22060	30.73	232.2	464.40	colon tumor	2.27
colon normal GW98-561	23514	31.25	170.2	340.40	colon normal
colon tumor GW98-560	23513	25.38	5486.82	10973.64	colon tumor	32.24
colon normal GW98-894	24691	31.16	180.03	360.06	colon normal
colon tumor GW98-893	24690	31.44	151.77	303.54	colon tumor	−1.19
lung normal GW98-3	20742	26.23	3322.51	6645.02	lung normal
lung tumor GW98-2	20741	28.13	1078.21	2156.42	lung tumor	−3.08
lung normal GW97-179	20677	28.53	847.92	1695.84	lung normal
lung tumor GW97-178	20676	25.6	4821.54	9643.08	lung tumor	5.69
lung normal GW98-165	21922	26.55	2749.53	5499.06	lung normal
lung tumor GW98-164	21921	25.19	6129.4	12258.80	lung tumor	2.23
lung normal GW98-282	22584	26.14	3498.08	6996.16	lung normal
lung tumor GW98-281	22583	28.57	832.53	1665.06	lung tumor	−4.20
breast normal GW00-392	28750	29.87	385.61	385.61	breast normal
breast tumor GW00-391	28746	30.46	272.26	544.52	breast tumor	1.41
breast normal GW00-413	28798	30.67	240.34	240.34	breast normal
breast tumor GW00-412	28797	30.48	268.96	537.92	breast tumor	2.24
breast normal GW00-	27592-95	30.45	273.76	273.76	breast normal
235:238
breast tumor GW00-	27588-91	30.21	315.33	315.33	breast tumor	1.15
231:234
breast normal GW98-621	23656	29.69	427.33	854.66	breast normal
breast tumor GW98-620	23655	30.06	345.05	690.10	breast tumor	−1.24
brain normal BB99-542	25507	27.8	1311.06	2622.12	brain normal
brain normal BB99-406	25509	27.43	1626.56	3253.12	brain normal
brain normal BB99-904	25546	30.13	330.31	660.62	brain normal
brain stage 5	25502	29.06	622.62	1245.24	brain stage 5 ALZ	−1.75
ALZ BB99-874
brain stage 5	25503	27.07	2020.76	4041.52	brain stage 5 ALZ	1.86
ALZ BB99-887
brain stage 5	25504	28.16	1058.41	2116.82	brain stage 5 ALZ	−1.03
ALZ BB99-862
brain stage 5	25542	28.29	978.87	1957.74	brain stage 5 ALZ	−1.11
ALZ BB99-927
CT lung	normal	28.86	699.64	1399.28	CT lung Nml
lung 26	normal	27.67	1416.44		lung 26 Nml
lung 27	normal	31.28	167.59	167.59	lung 27 Nml
lung 24	COPD	31.37	158.47	158.47	lung 24 COPD	4.02
lung 28	COPD	31.09	187.38	187.38	lung 28 COPD	−3.40
lung 23	COPD	29.49	482.41	482.41	lung 23 COPD	−1.32
lung 25	normal	30.05	346.64	346.64	lung 25 Nml
asthmatic lung ODO3112	29321	28.32	965.18	965.18	asthmatic lung	1.51
asthmatic lung ODO3433	29323	27.74	1354.63	2709.26	asthmatic lung	4.25
asthmatic lung ODO3397	29322	28.08	1112.71	2225.42	asthmatic lung	3.49
asthmatic lung ODO4928	29325	28.13	1075.22	2150.44	asthmatic lung	3.37
endo cells	control	40	0	0.00	endo cells
endo VEGF		35.79	11.64	11.64	endo VEGF	11.64
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	35.89	10.93	21.86	heart
heart (T-1) ischemic	29417	31.1	186.02	372.04	heart (T-1)	17.02
					ischemic
heart (T-14) non-	29422	31.91	115.38	230.76	heart (T-14) non-	10.56
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	30.71	234.8	469.60	heart (T-3399)	21.48
					DCM
adenoid GW99-269	26162	27.78	1325.3	2650.60	adenoid
tonsil GW98-280	22582	26.94	2181.1	4362.20	tonsil
T cells PC00314	28453	30.14	328.5	657.00	T cells
PBMNC		34.06	32.41	32.41	PBMNC
monocyte		32.1	103.22	206.44	monocyte
B cells PC00665	28455	30.49	266.46	532.92	B cells
dendritic cells	28441	29.8	400.89	801.78	dendritic cells
neutrophils	28440	28.31	966.8	966.80	neutrophils
eosinophils	28446	29.5	478.06	956.12	eosinophils
BM unstim		31.09	187.47	187.47	BM unstim
BM stim	treated	31.53	144.53	144.53	BM stim	−1.30
osteo dif	treated	36.7	6.78	6.78	osteo dif	1.20
osteo undif		37	5.67	5.67	osteo undif
chondrocytes		33.79	37.96	94.90	chondrocytes
OA Synovium IP12/01	29462	29.29	543.87	543.87	OA Synovium
OA Synovium NP10/01	29461	30.73	231.25	462.50	OA Synovium
OA Synovium NP57/00	28464	30.01	354.05	708.10	OA Synovium
RA Synovium	28466	30.19	319.47	638.94	RA Synovium
NP03/01
RA Synovium	28467	29.59	454.45	908.90	RA Synovium
NP71/00
RA Synovium	28475	30.26	306.51	613.02	RA Synovium
NP45/00
OA bone (biobank)	29217	30.44	274.99	274.99	OA bone
					(biobank)
OA bone Sample 1	J. Emory	30.58	253.73	507.46	OA bone
OA bone Sampl 2	J. Emory	29.5	478.67	957.34	OA bone
Cartilage (pool)	Normal	29.84	392.34	784.68	Nml Cartilage
					(pool)
Cartilage (pool)	OA	32.44	84.12	168.24	OA Cartilage	−4.66
					(pool)
PBL unifected	28441	30.99	198.67	397.34	PBL unifected
PBL HIV IIIB	28442	31.5	146.74	293.48	PBL HIV IIIB	−1.35
MRC5 uninfected (100%)	29158	34.39	26.52	53.04	MRC5
					uninfected
					(100%)
MRC5 HSV strain F	29178	35.37	14.92	29.84	MRC5 HSV	−1.78
					strain F
W12 cells	29179	30.15	325.42	650.84	W12 cells	−1.01
Keratinocytes	29180	30.14	327.34	654.68	Keratinocytes
B-actin control		28.07	1118.54
genomic		27.26	1802.46
1.00E+05		20.95	100000
1.00E+05		20.95	100000
1.00E+04		24.15	10000
1.00E+04		23.99	10000
1.00E+03		27.62	1000
1.00E+03		27.5	1000
1.00E+02		32.65	100
1.00E+02		32.29	100
1.00E+01		36.41	10
1.00E+01		40	0
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0187] Gene Name sbg1563294acadherin

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.71
colon tumor                      2.27
colon tumor                      32.24
colon tumor                      −1.19
lung tumor                       −3.08
lung tumor                       5.69
lung tumor                       2.23
lung tumor                       −4.20
breast tumor                     1.41
breast tumor                     2.24
breast tumor                     1.15
breast tumor                     −1.24
brain stage 5 ALZ                −1.75
brain stag 5 ALZ                 1.86
brain stage 5 ALZ                −1.03
brain stage 5 ALZ                −1.11
lung 24 COPD                     −4.02
lung 28 COPD                     −3.40
lung 23 COPD                     −1.32
asthmatic lung                   1.51
asthmatic lung                   4.25
asthmatic lung                   3.49
asthmatic lung                   3.37
endo VEGF                        11.64
endo bFGF                        0.00
heart (T-1) ischemia             17.02
heart (T-14) non-obstructive DCM 10.56
heart (T-3399) DCM               21.48
BM stim                          −1.30
osteo dif                        1.20
OA Cartilage (pool)              −4.66
PBL HIV IIIB                     −1.35
MRC5 HSV strain F                −1.78
W12 cells                        −1.01

[0188] Gene Name sbg1563294bcadherin

[0189] Moderate overall expression. The highest normal expression is seen in the adrenal gland, whole brain, fetal brain, pancreas, hypothalamus, and the testis. Additional high expression is seen in the endometrium, esophagus, heart, liver, pancreas, rectum, and small intestine. This normal expression pattern suggests that this gene may be involved in certain metabolic disorders. The highest disease expression is seen in some of the colon, lung, and breast normal and tumor samples as well as in the asthmatic lung samples and the adenoid and tonsil. Large upregulation in 1 of 4 colon tumor samples is sufficient to make a claim for colon cancer. Upregulation in 1 of 4 lung tumor samples implicates this gene in cancer of the lung. Upregulation in 3 of 4 asthmatic lung samples coupled with immune cell expression in indicates a possible involvement in asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV. This gene appears to show the same expression patterns in the disease samples as 1563294a. Based on this, it is likely that these two different primer sets are amplifying the same gene.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1563294b-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
cadherin	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	37.49, 35.73	1.36	4.27	2.82	3.06	16.34	46.00
Adipocytes Zenbio
Subcutaneous Adipose	35.02, 36.17	6.8	3.22	5.01	0.96	52.36	262.30
Zenbio
Adrenal Gland Clontech	33.03, 33.13	25.03	23.45	24.24	0.61	81.97	1986.89
Whole Brain Clontech	28.46, 28.38	494.81	521.62	508.22	7.24	6.91	3509.77
Fetal Brain Clontech	33.31, 33.77	20.82	15.42	18.12	0.48	103.95	1883.58
Cerebellum Clontech	34.14, 34	12.12	13.22	12.67	2.17	23.04	291.94
Cervix	34.5, 35.14	9.55	6.29	7.92	2.42	20.66	163.64
Colon	34.01, 33.67	13.2	16.39	14.80	2.71	18.45	272.97
Endometrium	34.01, 34.27	13.17	11.12	12.15	0.73	68.21	828.44
Esophagus	33.36, 33.24	20.16	21.74	20.95	1.37	36.50	764.60
Heart Clontech	33.64, 33.59	16.73	17.32	17.03	1.32	37.88	644.89
Hypothalamus	34.93, 33.8	7.24	15.11	11.18	0.32	155.28	1735.25
Ileum	34.29, 34.04	10.98	12.94	11.96	2.58	19.38	231.78
Jejunum	33.16, 35	22.96	6.91	14.94	6.60	7.58	113.14
Kidney	34.08, 34.26	12.61	11.2	11.91	2.12	23.58	280.78
Liver	33.38, 33.12	19.84	23.61	21.73	1.50	33.33	724.17
Fetal Liver Clontech	33.88, 33.28	14.33	21.14	17.74	10.40	4.81	85.26
Lung	33.73, 33.67	15.81	16.42	16.12	2.57	19.46	313.52
Mammary Gland	34.75, 34.81	8.1	7.79	7.95	13.00	3.85	30.56
Clontech
Myometrium	33.89, 34.25	14.25	11.26	12.76	2.34	21.37	272.54
Omentum	34.44, 34.42	9.95	10.06	10.01	3.94	12.69	126.97
Ovary	33.31, 33.26	20.76	21.54	21.15	4.34	11.52	243.66
Pancreas	33.39, 33.86	19.76	14.5	17.13	0.81	61.80	1058.71
Head of Pancreas	34.03, 34.1	13.03	12.44	12.74	1.57	31.85	405.57
Parotid Gland	34.76, 34.14	8.09	12.07	10.08	5.48	9.12	91.97
Placenta Clontech	34.55, 33.51	9.24	18.23	13.74	5.26	9.51	130.56
Prostate	33.38, 33.86	19.9	14.52	17.21	3.00	16.67	286.83
Rectum	33.28, 33.69	21.26	16.22	18.74	1.23	40.65	761.79
Salivary Gland Clontech	34, 34.3	13.23	10.87	12.05	7.31	6.84	82.42
Skeletal Muscle	33.67, 33.98	16.45	13.38	14.92	1.26	39.68	591.87
Clontech
Skin	34.09, 34.16	12.45	11.9	12.18	1.21	41.32	503.10
Small Intestine Clontech	33.85, 33.54	14.59	17.86	16.23	0.98	51.07	828.65
Spleen	33.41, 34.42	19.48	10.04	14.76	4.92	10.16	150.00
Stomach	33.27, 34.25	21.28	11.24	16.26	2.73	18.32	297.80
Testis Clontech	33.14, 33.18	23.23	22.66	22.95	0.57	87.87	2016.26
Thymus Clontech	31.43, 31.74	70.97	58.17	64.57	9.89	5.06	326.44
Thyroid	34.02, 33.55	13.09	17.78	15.44	2.77	18.05	278.61
Trachea Clontech	33.46, 33.58	18.81	17.38	18.10	9.71	5.15	93.18
Urinary Bladder	34.01, 33.99	13.16	13.32	13.24	5.47	9.14	121.02
Uterus	32.8, 33.11	29	23.72	26.36	5.34	9.36	246.82
genomic	27.37	1004.18
b-actin	28.24	569.86
1.00E+05	20.35	100000
1.00E+05	20.42	100000
1.00E+04	23.72	10000
1.00E+04	23.91	10000
1.00E+03	27.31	1000
1.00E+03	27.29	1000
1.00E+02	31.21	100
1.00E+02	30.74	100
1.00E+01	33.19	−1
1.00E+01	33.15	−1
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1563294b-	(GSK		GOI	total		Disease
cadherin	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	30.36	297.32	594.64	colon normal
colon tumor GW98-166	21940	30.25	316.19	632.38	colon tumor	1.06
colon normal GW98-178	22080	32.94	68.78	137.56	colon normal
colon tumor GW98-177	22060	31.16	189.21	378.42	colon tumor	2.75
colon normal GW98-561	23514	31.86	126.77	253.54	colon normal
colon tumor GW98-560	23513	25.95	3631.23	7262.46	colon tumor	28.64
colon normal GW98-894	24691	31.23	181.79	363.58	colon normal
colon tumor GW98-893	24690	32.39	93.93	187.86	colon tumor	−1.94
lung normal GW98-3	20742	26.77	2287.7	4575.40	lung normal
lung tumor GW98-2	20741	28.7	763.18	1526.36	lung tumor	−3.00
lung normal GW97-179	20677	29.12	600.92	1201.84	lung normal
lung tumor GW97-178	20676	26.39	2830.92	5661.84	lung tumor	4.71
lung normal GW98-165	21922	27.18	1811.38	3622.76	lung normal
lung tumor GW98-164	21921	25.7	4187.41	8374.82	lung tumor	2.31
lung normal GW98-282	22584	26.43	2772.29	5544.58	lung normal
lung tumor GW98-281	22583	29.05	624.92	1249.84	lung tumor	−4.44
breast normal GW00-392	28750	30.07	351.94	351.94	breast normal
breast tumor GW00-391	28746	30.38	294.08	588.16	breast tumor	1.67
breast normal GW00-413	28798	31.2	185.02	185.02	breast normal
breast tumor GW00-412	28797	30.11	343.21	686.42	breast tumor	3.71
breast normal GW00-	27592-95	31.1	195.26	195.26	breast normal
235:238
breast tumor GW00-	27588-91	30.83	228.5	228.50	breast tumor	1.17
231:234
breast normal GW98-621	23656	30.07	350.88	701.76	breast normal
breast tumor GW98-620	23655	31.01	205.73	411.46	breast tumor	−1.71
brain normal BB99-542	25507	28.3	958.12	1916.24	brain normal
brain normal BB99-406	25509	27.98	1148.55	2297.10	brain normal
brain normal BB99-904	25546	30.32	304.77	609.54	brain normal
brain stage 5 ALZ BB99-	25502	29.57	465.37	930.74	brain stage 5 ALZ	−1.73
874
brain stage 5 ALZ BB99-	25503	27.37	1624.72	3249.44	brain stage 5 ALZ	2.02
887
brain stage 5 ALZ BB99-	25504	28.23	1000.08	2000.16	brain stage 5 ALZ	1.24
862
brain stage 5 ALZ BB99-	25542	28.66	783.14	1566.28	brain stage 5 ALZ	−1.03
927
CT lung	normal	28.86	698.25	1396.50	CT lung Nml
lung 26	normal	29.32	536.67		lung 26 Nml
lung 27	normal	32.23	102.91	102.91	lung 27 Nml
lung 24	COPD	32.82	73.51	73.51	lung 24 COPD	−7.79
lung 28	COPD	32.07	113.08	113.08	lung 28 COPD	−5.06
lung 23	COPD	30.17	330.88	330.88	lung 23 COPD	−1.73
lung 25	normal	30.91	217.57	217.57	lung 25 Nml
asthmatic lung	29321	28.81	716.54	716.54	asthmatic lung	1.25
ODO3112
asthmatic lung	29323	27.55	1463.97	2927.94	asthmatic lung	5.12
ODO3433
asthmatic lung	29322	28.04	1112.34	2224.68	asthmatic lung	3.89
ODO3397
asthmatic lung	29325	28.08	1084.62	2169.24	asthmatic lung	3.79
ODO4928
endo cells	control	40	0	0.00	endo cells
endo VEGF		40	0	0.00	endo VEGF	0.00
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	35.23	18.78	37.56	heart
heart (T-1) ischemic	29417	31.14	191.53	383.06	heart (T-1)	10.20
					ischemic
heart (T-14) non-	29422	31.71	138.24	276.48	heart (T-14) non-	7.36
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	30.96	211.99	423.98	heart (T-3399)	11.29
					DCM
adenoid GW99-269	26162	27.86	1230.52	2461.04	adenoid
tonsil GW98-280	22582	26.75	2315.5	4631.00	tonsil
T cells PC00314	28453	30.22	322.85	645.70	T cells
PBMNC		34.32	31.53	31.53	PBMNC
monocyte		32.88	71.25	142.50	monocyte
B cells PC00665	28455	30.67	250.35	500.70	B cells
dendritic cells	28441	30.26	314.6	629.20	dendritic cells
neutrophils	28440	28.51	851.25	851.25	neutrophils
eosinophils	28446	30.2	326.41	652.82	eosinophils
BM unstim		31.78	133.15	133.15	BM unstim
BM stim	treated	31.77	133.52	133.52	BM stim	1.00
osteo dif	treated	37.28	5.86	5.86	osteo dif	−1.16
osteo undif		37.03	6.77	6.77	osteo undif
chondrocytes		33.06	64.39	160.98	chondrocytes
OA Synovium IP12/01	29462	29.57	465.45	465.45	OA Synovium
OA Synovium NP10/01	29461	31.15	190.41	380.82	OA Synovium
OA Synovium NP57/00	28464	30.1	346.02	692.04	OA Synovium
RA Synovium	28466	30.81	230.7	461.40	RA Synovium
NP03/01
RA Synovium	28467	29.68	438.8	877.60	RA Synovium
NP71/00
RA Synovium	28475	30.54	269.5	539.00	RA Synovium
NP45/00
OA bone (biobank)	29217	31.25	179.99	179.99	OA bone
					(biobank)
OA bone Sample 1	J. Emory	30.88	222.01	444.02	OA bone
OA bone Sample 2	J. Emory	29.91	384.53	769.06	OA bone
Cartilage (pool)	Normal	30.22	322.99	645.98	Nml Cartilage
					(pool)
Cartilage (pool)	OA	32.9	70.57	141.14	OA Cartilage	−4.58
					(pool)
PBL unifected	28441	30.47	280.4	560.80	PBL unifected
PBL HIV IIIB	28442	31.96	120.33	240.66	PBL HIV IIIB	−2.33
MRC5 uninfected	29158	34.5	28.48	56.96	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	40	0	0.00	MRC5 HSV	−56.96
					strain F
W12 cells	29179	30.42	288.67	577.34	W12 cells	1.07
Keratinocytes	29180	30.54	268.96	537.92	Keratinocytes
B-actin control		27.74	1315.45
genomic		27.18	1811.29
1.00E+05		20.88	100000
1.00E+05		20.75	100000
1.00E+04		23.99	10000
1.00E+04		23.9	10000
1.00E+03		27.54	1000
1.00E+03		27.31	1000
1.00E+02		31.42	100
1.00E+02		32.09	100
1.00E+01		38.05	10
1.00E+01		40	0
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0190] Gene Name sbg1563294bcadherin

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.06
colon tumor                      2.75
colon tumor                      28.64
colon tumor                      −1.94
lung tumor                       −3.00
lung tumor                       4.71
lung tumor                       2.31
lung tumor                       −4.44
breast tumor                     1.67
breast tumor                     3.71
breast tumor                     1.17
breast tumor                     −1.71
brain stage 5 ALZ                −1.73
brain stage 5 ALZ                2.02
brain stage 5 ALZ                1.24
brain stage 5 ALZ                −1.03
lung 24 COPD                     −7.79
lung 28 COPD                     −5.06
lung 23 COPD                     −1.73
asthmatic lung                   1.25
asthmatic lung                   5.12
asthmatic lung                   3.89
asthmatic lung                   3.79
endo VEGF                        0.00
endo bFGF                        0.00
heart (T-1) ischemic             10.20
heart (T-14) non-obstructive DCM 7.36
heart (T-3399) DCM               11.29
BM stim                          1.00
osteo dif                        −1.16
OA Cartilage (pool)              −4.58
PBL HIV IIIB                     −2.33
MRC5 HSV strain F                −56.96
W12 cells                        1.07

[0191] Gene Name sbg1563509ECM

[0192] Moderate to high overall expression. This gene is expressed fairly ubiquitously throughout most of the normal tissues. The highest normal expression is seen in the lung, fetal liver, and mammary gland while the lowest normal expression is seen in the adrenal, fetal brain, hypothalamus, head of pancreas, skeletal muscle, and the testis. The highest disease expression is seen in some of the normal and tumor lung samples and in the OA and RA synovium samples. Downregulation in 1 of 4 lung tumor samples suggests a potential role in cancer of the lung. Upregulation of 1 of 4 breast tumor samples implicates this gene in breast cancer. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV. High expression in the OA and RA synovium samples with corroborating immune cell expression implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1563509ECM	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	30.38, 30.22	142.77	157.17	149.97	3.06	16.34	2450.49
Adipocytes Zenbio
Subcutaneous Adipose	33.18, 33.06	25.38	27.32	26.35	0.96	52.36	1379.58
Zenbio
Adrenal Gland Clontech	40, 40	0	0.66	0.33	0.61	81.97	27.05
Whole Brain Clontech	28.64, 28.64	416.91	417.36	417.14	7.24	6.91	2880.77
Fetal Brain Clontech	40, 40	0.61	0	0.31	0.48	103.95	31.70
Cerebellum Clontech	40, 37.11	0	2.26	1.13	2.17	23.04	26.04
Cervix	31.79, 31.96	59.71	53.85	56.78	2.42	20.66	1173.14
Colon	32.07, 31.6	50.27	67.25	58.76	2.71	18.45	1084.13
Endometrium	33.39, 35.81	22.3	5.02	13.66	0.73	68.21	931.79
Esophagus	32.84, 33.08	31.34	27.05	29.20	1.37	36.50	1065.51
Heart Clontech	32.16, 32.92	47.54	29.78	38.66	1.32	37.88	1464.39
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	30.79, 30.95	111.17	100.57	105.87	2.58	19.38	2051.74
Jejunum	30.98, 30.35	98.76	145.23	122.00	6.60	7.58	924.20
Kidney	33.39, 32.68	22.36	34.55	28.46	2.12	23.58	671.11
Liver	33.58, 32.98	19.81	28.77	24.29	1.50	33.33	809.67
Fetal Liver Clontech	27.68, 27.62	752.33	780.99	766.66	10.40	4.81	3685.87
Lung	29.72, 29.24	214.26	288.46	251.36	2.57	19.46	4890.27
Mammary Gland	27.58, 27.41	802.77	889.8	846.29	13.00	3.85	3254.94
Clontech
Myometrium	31.3, 31.26	80.88	82.8	81.84	2.34	21.37	1748.72
Omentum	31.16, 30.49	88.32	133.39	110.86	3.94	12.69	1406.79
Ovary	29.81, 29.17	202.59	300.28	251.44	4.34	11.52	2896.72
Pancreas	34.04, 34.58	14.94	10.75	12.85	0.81	61.80	793.88
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	30.91, 31.08	102.7	92.68	97.69	5.48	9.12	891.33
Placenta Clontech	31.84, 31.93	58.07	54.86	56.47	5.26	9.51	536.74
Prostate	34.37, 34.51	12.24	11.17	11.71	3.00	16.67	195.08
Rectum	32.07, 32.38	50.41	41.58	46.00	1.23	40.65	1869.72
Salivary Gland Clontech	32.14, 31.5	48.38	71.44	59.91	7.31	6.84	409.78
Skeletal Muscle	40, 34.72	0	9.81	4.91	1.26	39.68	194.64
Clontech
Skin	34.2, 33.36	13.55	22.72	18.14	1.21	41.32	749.38
Small Intestine Clontech	40, 35.18	0.59	7.41	4.00	0.98	51.07	204.29
Spleen	30.31, 30.31	149.25	149.43	149.34	4.92	10.16	1517.68
Stomach	33.04, 33.16	27.79	25.75	26.77	2.73	18.32	490.29
Testis Clontech	40, 40	0.94	0.74	0.84	0.57	87.87	73.81
Thymus Clontech	29.38, 29.2	263.89	296.27	280.08	9.89	5.06	1415.98
Thyroid	31.72, 31.9	62.48	55.9	59.19	2.77	18.05	1068.41
Trachea Clontech	30.31, 29.88	148.77	194.55	171.66	9.71	5.15	883.93
Urinary Bladder	32.11, 31.47	49.26	72.88	61.07	5.47	9.14	558.23
Uterus	31.42, 30.77	75.27	112.11	93.69	5.34	9.36	877.25
genomic	26.84	1263.86
b-actin	28.08	588.35
1.00E+05	19.86	100000
1.00E+05	20	100000
1.00E+04	23.16	10000
1.00E+04	23.17	10000
1.00E+03	26.87	1000
1.00E+03	26.65	1000
1.00E+02	32.21	100
1.00E+02	32.05	100
1.00E+01	40	10
1.00E+01	33.56	10
1.00E+00	40	1
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1563509ECM	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	26.32	2332.21	4664.42	colon normal
colon tumor GW98-166	21940	25.44	3916.42	7832.84	colon tumor	1.68
colon normal GW98-178	22080	28.98	482.04	964.08	colon normal
colon tumor GW98-177	22060	29.93	275.13	550.26	colon tumor	−1.75
colon normal GW98-561	23514	29.66	322.03	644.06	colon normal
colon tumor GW98-560	23513	28.47	651.69	1303.38	colon tumor	2.02
colon normal GW98-894	24691	28.51	635.18	1270.36	colon normal
colon tumor GW98-893	24690	29.67	320.97	641.94	colon tumor	−1.98
lung normal GW98-3	20742	23.5	12376.95	24753.90	lung normal
lung tumor GW98-2	20741	29.25	409.4	818.80	lung tumor	−30.23
lung normal GW97-179	20677	25.12	4727.38	9454.76	lung normal
lung tumor GW97-178	20676	23.65	11323.07	22646.14	lung tumor	2.40
lung normal GW98-165	21922	24.12	8559.3	17118.60	lung normal
lung tumor GW98-164	21921	24.13	8529.62	17059.24	lung tumor	−1.00
lung normal GW98-282	22584	26.18	2520.39	5040.78	lung normal
lung tumor GW98-281	22583	24.28	7787.44	15574.88	lung tumor	3.09
breast normal GW00-392	28750	25.24	4414.62	4414.62	breast normal
breast tumor GW00-391	28746	26.18	2521.92	5043.84	breast tumor	1.14
breast normal GW00-413	28798	28	859.75	859.75	breast normal
breast tumor GW00-412	28797	28.18	773.07	1546.14	breast tumor	1.80
breast normal GW00-	27592-95	29.09	451.71	451.71	breast normal
235:238
breast tumor GW00-	27588-91	25.21	4489.31	4489.31	breast tumor	9.94
231:234
breast normal GW98-621	23656	24.01	9115.61	18231.22	breast normal
breast tumor GW98-620	23655	26.31	2333.67	4667.34	breast tumor	−3.91
brain normal BB99-542	25507	31.35	118.13	236.26	brain normal
brain normal BB99-406	25509	31.59	103.02	206.04	brain normal
brain normal BB99-904	25546	32.23	70.46	140.92	brain normal
brain stage 5 ALZ BB99-	25502	31.03	143.12	286.24	brain stage 5 ALZ	1.47
874
brain stage 5 ALZ BB99-	25503	30.04	257.7	515.40	brain stage 5 ALZ	2.65
887
brain stage 5 ALZ BB99-	25504	30.68	176.24	352.48	brain stage 5 ALZ	1.81
862
brain stage 5 ALZ BB99-	25542	30.85	158.78	317.56	brain stage 5 ALZ	1.63
927
CT lung	normal	26.51	2080.42	4160.84	CT lung Nml
lung 26	normal	27.53	1136.99		lung 26 Nml
lung 27	normal	27.62	1076.86	1076.86	lung 27 Nml
lung 24	COPD	27.67	1044.37	1044.37	lung 24 COPD	−2.14
lung 28	COPD	27.31	1293.45	1293.45	lung 28 COPD	−1.73
lung 23	COPD	27.73	1008.19	1008.19	lung 23 COPD	−2.22
lung 25	normal	27.09	1476.73	1476.73	lung 25 Nml
asthmatic lung ODO3112	29321	28.59	607.37	607.37	asthmatic lung	−3.68
asthmatic lung ODO3433	29323	26.78	1774.99	3549.98	asthmatic lung	1.59
asthmatic lung ODO3397	29322	26	2814.56	5629.12	asthmatic lung	2.52
asthmatic lung ODO4928	29325	26.69	1867.05	3734.10	asthmatic lung	1.67
endo cells	control	29.39	377.18	377.18	endo cells
endo VEGF		29.2	422.38	422.38	endo VEGF	1.12
endo bFGF		30.87	157.65	157.65	endo bFGF	−2.39
heart Clontech	normal	30.26	225.09	450.18	heart
heart (T-1) ischemic	29417	26.68	1885.2	3770.40	heart (T-1)	8.38
					ischemic
heart (T-14) non-	29422	26.44	2160.24	4320.48	heart (T-14) non-	9.60
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	26.04	2749.86	5499.72	heart (T-3399)	12.22
					DCM
adenoid GW99-269	26162	28.03	842.76	1685.52	adenoid
tonsil GW98-280	22582	27.99	867.51	1735.02	tonsil
T cells PC00314	28453	35.16	12.44	24.88	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		35.75	8.76	17.52	monocyte
B cells PC00665	28455	36.19	6.74	13.48	B cells
dendritic cells	28441	40	0	0.00	dendritic cells
neutrophils	28440	33.4	35.17	35.17	neutrophils
eosinophils	28446	34.21	21.78	43.56	eosinophils
BM unstim		35.58	9.7	9.70	BM unstim
BM stim	treated	34.26	21.08	21.08	BM stim	2.17
osteo dif	treated	29.54	345.54	345.54	osteo dif	−1.27
osteo undif		29.14	438.01	438.01	osteo undif
chondrocytes		26.03	2766.86	6917.15	chondrocytes
OA Synovium IP12/01	29462	24.15	8414.27	8414.27	OA Synovium
OA Synovium NP10/01	29461	25.17	4607.95	9215.90	OA Synovium
OA Synovium NP57/00	28464	23.55	11981.42	23962.84	OA Synovium
RA Synovium	28466	24.98	5127.1	10254.20	RA Synovium
NP03/01
RA Synovium	28467	24.22	8086.53	16173.06	RA Synovium
NP71/00
RA Synovium	28475	25.08	4839.85	9679.70	RA Synovium
NP45/00
OA bone (biobank)	29217	28.59	605.78	605.78	OA bone
					(biobank)
OA bone Sample 1	J. Emory	27.67	1043.14	2086.28	OA bone
OA bone Sample 2	J. Emory	27.8	969.07	1938.14	OA bone
Cartilage (pool)	Normal	26.06	2714.12	5428.24	Nml Cartilage
					(pool)
Cartilage (pool)	OA	26.45	2156.8	4313.60	OA Cartilage	−1.26
					(pool)
PBL unifected	28441	29.88	282.45	564.90	PBL unifected
PBL HIV IIIB	28442	34.32	20.4	40.80	PBL HIV IIIB	−13.85
MRC5 uninfected	29158	29.36	385.52	771.04	MRC5
(100%)					uninfected
					(100%)
MRC5 HSV strain F	29178	32.49	60.36	120.72	MRC5 HSV	−6.39
					strain F
W12 cells	29179	32.1	75.78	151.56	W12 cells	−1.30
Keratinocytes	29180	31.66	98.45	196.90	Keratinocytes
B-actin control		28.06	828.85
genomic		27.22	1362.28
1.00E+05		20.1	100000
1.00E+05		20.32	100000
1.00E+04		23.72	10000
1.00E+04		23.67	10000
1.00E+03		27.61	1000
1.00E+03		27.29	1000
1.00E+02		32.12	100
1.00E+02		31.32	100
1.00E+01		40	0
1.00E+01		35.78	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0193] Gene Name sbg1563509ECM

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.68
colon tumor                      −1.75
colon tumor                      2.02
colon tumor                      −1.98
lung tumor                       −30.23
lung tumor                       2.40
lung tumor                       −1.00
lung tumor                       3.09
breast tumor                     1.14
breast tumor                     1.80
breast tumor                     9.94
breast tumor                     −3.91
brain stage 5 ALZ                1.47
brain stage 5 ALZ                2.65
brain stage 5 ALZ                1.81
brain stage 5 ALZ                1.63
lung 24 COPD                     −2.14
lung 28 COPD                     −1.73
lung 23 COPD                     −2.22
asthmatic lung                   −3.68
asthmatic lung                   1.59
asthmatic lung                   2.52
asthmatic lung                   1.67
endo VEGF                        1.12
endo bFGF                        −2.39
heart (T-1) ischemic             8.38
heart (T-14) non-obstructive DCM 9.60
heart (T-3399) DCM               12.22
BM stim                          2.17
osteo dif                        −1.27
OA Cartilage (pool)              −1.26
PBL HIV IIIB                     −13.85
MRC5 HSV strain F                −6.39
W12 cells                        −1.30

[0194] Gene Name sbg1552159Serprotease

[0195] Moderate overall expression. The highest normal expression is seen in the whole brain, fetal brain, thymus, and testis. The highest disease expression is seen in the some of the normal counterparts of both the lung and breast tumor samples as well as in three of the asthmatic lung samples. Significant downregulation in 1 of 4 lung tumor samples and 1 of 4 breast tumor samples is sufficient to claim involvement in lung and breast cancer. Upregulation in 3 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Upregulation in 3 of 4 asthmatic lung samples with corroborating high expression in the neutrophils indicates a possible involvement in asthma. Upregulated in the differentiated osteoblasts. Downregulation in the HIV-infected PBLs and the HSV-infected MRC5 cells suggests that this gene may be a host factor in HIV and HSV. Downregulation in the W12 cells suggests that this gene may be a host factor in HPV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1552159-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
Serprotease	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	36.04, 34.59	10.17	23.6	16.89	3.06	16.34	275.90
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0.16	0	0.08	0.96	52.36	4.19
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	27.89, 27.71	1131.62	1253.56	1192.59	7.24	6.91	8236.12
Fetal Brain Clontech	36.08, 35.62	9.95	13.02	11.49	0.48	103.95	1193.87
Cerebellum Clontech	37.52, 40	4.34	0	2.17	2.17	23.04	50.00
Cervix	36.03, 37.08	10.22	5.6	7.91	2.42	20.66	163.43
Colon	34.81, 40	20.71	0	10.36	2.71	18.45	191.05
Endometrium	40, 40	0	0	0.00	0.73	68.21	0.00
Esophagus	36.79	6.59	0	3.30	1.37	36.50	120.26
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	36.49, 36.39	7.84	8.33	8.09	2.58	19.38	156.69
Jejunum	32.83, 34.02	65.26	32.68	48.97	6.60	7.58	370.98
Kidney	36.08, 35.48	9.96	14.12	12.04	2.12	23.58	283.96
Liver	35.97, 40	10.62	20.57	15.60	1.50	33.33	519.83
Fetal Liver Clontech	33.01, 33.66	58.78	40.39	49.59	10.40	4.81	238.39
Lung	33.21, 33.19	52.28	52.86	52.57	2.57	19.46	1022.76
Mammary Gland	32.97, 32.13	60.1	97.47	78.79	13.00	3.85	303.02
Clontech
Myometrium	34.29, 33.57	28.1	42.41	35.26	2.34	21.37	753.31
Omentum	40, 35.08	0	17.79	8.90	3.94	12.69	112.88
Ovary	34.1, 33.78	31.22	37.67	34.45	4.34	11.52	396.83
Pancreas	36.9, 40	6.21	0	3.11	0.81	61.80	191.90
Head of Pancreas	40, 35.98	0	10.58	5.29	1.57	31.85	168.47
Parotid Gland	36.02, 36.29	10.33	8.8	9.57	5.48	9.12	87.27
Placenta Clontech	35.77, 40	11.89	0	5.95	5.26	9.51	56.51
Prostate	40, 36.13	0	9.7	4.85	3.00	16.67	80.83
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland Clontech	39.51, 35.16	1.37	16.91	9.14	7.31	6.84	62.52
Skeletal Muscle	36.29, 36.68	8.82	7.05	7.94	1.26	39.68	314.88
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	33.79, 35.77	37.52	11.89	24.71	4.92	10.16	251.07
Stomach	37.88, 37.81	3.53	3.67	3.60	2.73	18.32	65.93
Testis Clontech	35.14, 34.77	17.12	21.25	19.19	0.57	87.87	1685.85
Thymus Clontech	29.65, 29.15	409.27	547.64	478.46	9.89	5.06	2418.88
Thyroid	36.32, 40	8.64	0	4.32	2.77	18.05	77.98
Trachea Clontech	32.97, 35.24	60.19	16.17	38.18	9.71	5.15	196.60
Urinary Bladder	36.78, 36.33	6.65	8.61	7.63	5.47	9.14	69.74
Uterus	33.46, 33.09	45.17	55.91	50.54	5.34	9.36	473.22
genomic	27.52	1400.32
b-actin	28.07	1019.35
1.00E+05	20.41	100000
1.00E+05	20.42	100000
1.00E+04	23.86	10000
1.00E+04	24.03	10000
1.00E+03	27.72	1000
1.00E+03	27.91	1000
1.00E+02	32	100
1.00E+02	32.12	100
1.00E+01	36.2	10
1.00E+01	36.36	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	−1
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1552159-	(GSK		GOI	total		Disease
Serprotease	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	31.77	108.85	217.70	colon normal
colon tumor GW98-166	21940	32.95	56.73	113.46	colon tumor	−1.92
colon normal GW98-178	22080	34.14	29.37	58.74	colon normal
colon tumor GW98-177	22060	33.79	35.61	71.22	colon tumor	1.21
colon normal GW98-561	23514	31.99	96.4	192.80	colon normal
colon tumor GW98-560	23513	31.61	119.45	238.90	colon tumor	1.24
colon normal GW98-894	24691	32.6	68.97	137.94	colon normal
colon tumor GW98-893	24690	33.77	36.1	72.20	colon tumor	−1.91
lung normal GW98-3	20742	27.73	1017.76	2035.52	lung normal
lung tumor GW98-2	20741	34.28	27.21	54.42	lung tumor	−37.40
lung normal GW97-179	20677	31.15	153.55	307.10	lung normal
lung tumor GW97-178	20676	29.11	474.86	949.72	lung tumor	3.09
lung normal GW98-165	21922	27.22	1351.96	2703.92	lung normal
lung tumor GW98-164	21921	29.62	357.43	714.86	lung tumor	−3.78
lung normal GW98-282	22584	30.8	186.98	373.96	lung normal
lung tumor GW98-281	22583	28.49	670.41	1340.82	lung tumor	3.59
breast normal GW00-392	28750	30.06	280.28	280.28	breast normal
breast tumor GW00-391	28746	28.92	526.38	1052.76	breast tumor	3.76
breast normal GW00-413	28798	31.84	104.84	104.84	breast normal
breast tumor GW00-412	28797	32.12	90.02	180.04	breast tumor	1.72
breast normal GW00-	27592-95	33.35	45.65	45.65	breast normal
235:238
breast tumor GW00-	27588-91	31.57	121.62	121.62	breast tumor	2.66
231:234
breast normal GW98-621	23656	28.87	542.39	1084.78	breast normal
breast tumor GW98-620	23655	33.91	33.4	66.80	breast tumor	−16.24
brain normal BB99-542	25507	29.48	386.14	772.28	brain normal
brain normal BB99-406	25509	29.99	291.64	583.28	brain normal
brain normal BB99-904	25546	31.2	149.66	299.32	brain normal
brain stage 5 ALZ BB99-	25502	30.16	265.84	531.68	brain stage 5 ALZ	−1.04
874
brain stage 5 ALZ BB99-	25503	28.08	840	1680.00	brain stage 5 ALZ	3.05
887
brain stage 5 ALZ BB99-	25504	28.53	655.61	1311.22	brain stage 5 ALZ	2.38
862
brain stage 5 ALZ BB99-	25542	28.08	837.24	1674.48	brain stage 5 ALZ	3.04
927
CT lung	normal	30.05	282.31	564.62	CT lung Nml
lung 26	normal	30.85	181.53		lung 26 Nml
lung 27	normal	33.45	43.19	43.19	lung 27 Nml
lung 24	COPD	31.01	166.35	166.35	lung 24 COPD	−1.34
lung 28	COPD	32.83	60.69	60.69	lung 28 COPD	−3.68
lung 23	COPD	32.14	88.79	88.79	lung 23 COPD	−2.52
lung 25	normal	32.77	62.65	62.65	lung 25 Nml
asthmatic lung ODO3112	29321	32.77	62.83	62.83	asthmatic lung	−3.56
asthmatic lung ODO3433	29323	27.59	1100.3	2200.60	asthmatic lung	9.85
asthmatic lung ODO3397	29322	27.93	914.58	1829.16	asthmatic lung	8.18
asthmatic lung ODO4928	29325	29.21	449.51	899.02	asthmatic lung	4.02
endo cells	control	36.25	9.18	9.18	endo cells
endo VEGF		35.45	14.27	14.27	endo VEGF	1.55
endo bFGF		36	10.49	10.49	endo bFGF	1.14
heart Clontech	normal	32.44	75.33	150.66	heart
heart (T-1) ischemic	29417	32.2	86	172.00	heart (T-1)	1.14
					ischemic
heart (T-14) non-	29422	31.72	112.13	224.26	heart (T-14) non-	1.49
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	32.01	95.37	190.74	heart (T-3399)	1.27
					DCM
adenoid GW99-269	26162	32.57	70.26	140.52	adenoid
tonsil GW98-280	22582	32.18	87.15	174.30	tonsil
T cells PC00314	28453	36.33	8.79	17.58	T cells
PBMNC		35.19	16.42	16.42	PBMNC
monocyte		39.03	1.97	3.94	monocyte
B cells PC00665	28455	33.31	46.62	93.24	B cells
dendritic cells	28441	30.84	182.9	365.80	dendritic cells
neutrophils	28440	29.11	475.18	475.18	neutrophils
eosinophils	28446	32.09	91.35	182.70	eosinophils
BM unstim		32.79	62.06	62.06	BM unstim
BM stim	treated	35	18.25	18.25	BM stim	−3.40
osteo dif	treated	32.61	68.38	68.38	osteo dif	9.29
osteo undif		36.65	7.36	7.36	osteo undif
chondrocytes		33.66	38.41	96.03	chondrocytes
OA Synovium IP12/01	29462	29.28	431.45	431.45	OA Synovium
OA Synovium NP10/01	29461	31.12	156.26	312.52	OA Synovium
OA Synovium NP57/00	28464	30.2	260.25	520.50	OA Synovium
RA Synovium	28466	31.27	143.47	286.94	RA Synovium
NP03/01
RA Synovium	28467	29.55	372.02	744.04	RA Synovium
NP71/00
RA Synovium	28475	31.04	162.93	325.86	RA Synovium
NP45/00
OA bone (biobank)	29217	31.88	102.61	102.61	OA bone (biobank)
OA bone Sample 1	J. Emory	29.15	463.55	927.10	OA bone
OA bone Sample 2	J. Emory	32.22	85.12	170.24	OA bone
Cartilage (pool)	Normal	29.1	477.1	954.20	Nml Cartilage
					(pool)
Cartilage (pool)	OA	31.42	132.47	264.94	OA Cartilage (pool)	−3.60
PBL unifected	28441	35.78	11.86	23.72	PBL unifected
PBL HIV IIIB	28442	40	0	0.00	PBL HIV IIIB	−23.72
MRC5 uninfected	29158	29.8	323.53	647.06	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	32.5	72.82	145.64	MRC5 HSV strain	−4.44
					F
W12 cells	29179	34.63	22.39	44.78	W12 cells	−8.73
Keratinocytes	29180	30.72	195.4	390.80	Keratinocytes
B-actin control		27.32	1277.66
genomic		27.01	1519.42
1.00E+05		19.85	100000
1.00E+05		19.83	100000
1.00E+04		23.23	10000
1.00E+04		23.32	10000
1.00E+03		27.27	1000
1.00E+03		27.11	1000
1.00E+02		33.06	100
1.00E+02		31.78	100
1.00E+01		39.91	10
1.00E+01		40	0
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0196] Gene Name sbg1552159Serprotease

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.92
colon tumor                      1.21
colon tumor                      1.24
colon tumor                      −1.91
lung tumor                       −37.40
lung tumor                       3.09
lung tumor                       −3.78
lung tumor                       3.59
breast tumor                     3.76
breast tumor                     1.72
breast tumor                     2.66
breast tumor                     −16.24
brain stage 5 ALZ                −1.04
brain stage 5 ALZ                3.05
brain stage 5 ALZ                2.38
brain stage 5 ALZ                3.04
lung 24 COPD                     −1.34
lung 28 COPD                     −3.68
lung 23 COPD                     −2.52
asthmatic lung                   −3.56
asthmatic lung                   9.85
asthmatic lung                   8.18
asthmatic lung                   4.02
endo VEGF                        1.55
endo bFGF                        1.14
heart (T-1) ischemic             1.14
heart (T-14) non-obstructive DCM 1.49
heart (T-3399) DCM               1.27
BM stim                          −3.40
osteo dif                        9.29
OA Cartilage (pool)              −3.60
PBL HIV IIIB                     −23.72
MRC5 HSV strain F                −4.44
W12 cells                        −8.73

[0197] Gene Name sbg1551159thymosinb4

[0198] High overall expression. This gene is expressed fairly ubiquitously in the normal samples with the highest levels of expression seen in the whole brain, endometrium, myometrium, rectum, spleen, uterus, and thymus. This gene is also expressed fairly ubiquitously in the disease samples with the highest levels of expression seen in the uninfected and infected PBLs. Upregulation in 2 of 4 colon tumor samples and 1 of 4 breast tumor samples is sufficient to claim that this gene may be involved in cancers of the colon and breast. Upregulation in 1 of 4 asthmatic lung samples with corroborating high expression in all of the inflammatory immune cells indicates a possible involvement in asthma Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Downregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in all of the inflammatory immune cells implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1551159-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
thymosinb4	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	21.19, 21.24	4954.06	4785.48	4869.77	3.06	16.34	79571.41
Adipocytes Zenbio
Subcutaneous Adipose	25.39, 22.19	315.45	2561.93	1438.69	0.96	52.36	75324.08
Zenbio
Adrenal Gland Clontech	26.25, 24.62	180.52	522.51	351.52	0.61	81.97	28812.70
Whole Brain Clontech	17.83, 17.65	44488.84	50016.56	47252.70	7.24	6.91	326330.80
Fetal Brain Clontech	25.1, 25.27	381.07	343.03	362.05	0.48	103.95	37635.14
Cerebellum Clontech	23.91, 23.79	830.52	902.3	866.41	2.17	23.04	19963.36
Cervix	22.08, 21.63	2754.7	3698.84	3226.77	2.42	20.66	66668.80
Colon	21.03, 21.04	5474.84	5453.74	5464.29	2.71	18.45	100817.16
Endometrium	21.96, 21.86	2977.38	3184.04	3080.71	0.73	68.21	210143.93
Esophagus	23.64, 23.34	996.91	1210.04	1103.48	1.37	36.50	40272.81
Heart Clontech	26.02, 24.86	209.22	447.18	328.20	1.32	37.88	12431.82
Hypothalamus	27.41, 27.27	84.36	92.31	88.34	0.32	155.28	13716.61
Ileum	21.97, 21.54	2968.68	3928.49	3448.59	2.58	19.38	66833.04
Jejunum	20.44, 20.44	8089.14	8083.85	8086.50	6.60	7.58	61261.33
Kidney	23.14, 22.11	1375.78	2708.16	2041.97	2.12	23.58	48159.67
Liver	24.97, 24.79	416.5	467.07	441.79	1.50	33.33	14726.17
Fetal Liver Clontech	19.45, 19.34	15432.97	16557.73	15995.35	10.40	4.81	76900.72
Lung	21.26, 21.5	4731.17	4046.45	4388.81	2.57	19.46	85385.41
Mammary Gland	19.29, 19.26	17125.19	17464.02	17294.61	13.00	3.85	66517.71
Clontech
Myometrium	20.82, 20.28	6308.04	8972.5	7640.27	2.34	21.37	163253.63
Omentum	20.59, 20.26	7310.61	9084.23	8197.42	3.94	12.69	104028.17
Ovary	21.51, 21.22	4019.4	4853.94	4436.67	4.34	11.52	51113.71
Pancreas	26.01, 26.25	210.39	179.95	195.17	0.81	61.80	12062.42
Head of Pancreas	26.2, 25.66	185.8	265.23	225.52	1.57	31.85	7182.01
Parotid Gland	22.42, 21.99	2213.28	2931.58	2572.43	5.48	9.12	23471.08
Placenta Clontech	22.43, 22.48	2194.88	2126.18	2160.53	5.26	9.51	20537.36
Prostate	22.82, 22.96	1695.79	1547.57	1621.68	3.00	16.67	27028.00
Rectum	22.21, 22.05	2541.48	2810.64	2676.06	1.23	40.65	108782.93
Salivary Gland Clontech	22.03, 21.67	2852.39	3620.4	3236.40	7.31	6.84	22136.76
Skeletal Muscle	27.39, 27.13	85.16	100.96	93.06	1.26	39.68	3692.86
Clontech
Skin	24.27, 23.9	656.73	838.66	747.70	1.21	41.32	30896.49
Small Intestin Clontech	26.86, 26.49	121.18	154.25	137.72	0.98	51.07	7033.45
Spleen	19.49, 19.43	15003.18	15611.73	15307.46	4.92	10.16	155563.57
Stomach	22.35, 21.82	2318.26	3278.55	2798.41	2.73	18.32	51252.84
Testis Clontech	28.06, 27.47	54.99	81.19	68.09	0.57	87.87	5983.30
Thymus Clontech	17.45, 17.18	57052.5	68169.71	62611.11	9.89	5.06	316537.44
Thyroid	23.74, 23.27	931.95	1264.88	1098.42	2.77	18.05	19826.99
Trachea Clontech	21.56, 21.25	3883.73	4744.74	4314.24	9.71	5.15	22215.42
Urinary Bladder	20.54, 20.19	7555.88	9539.87	8547.88	5.47	9.14	78134.14
Uterus	19.53, 19.13	14625.16	19031.66	16828.41	5.34	9.36	157569.38
genomic	23.18	1347.95
b-actin	26.19	186.76
1.00E+05	17.13	100000
1.00E+05	17.01	100000
1.00E+04	19.78	10000
1.00E+04	19.89	10000
1.00E+03	23.33	1000
1.00E+03	23.21	1000
1.00E+02	27.56	100
1.00E+02	27.4	100
1.00E+01	29.22	10
1.00E+01	30.45	10
1.00E+00	35.35	1
1.00E+00	34.35	1
NTC	40	0
NTC	34.73	−1
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1551159-	(GSK		GOI	total		Disease
thymosinb4	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	16.75	198628.18	397256.36	colon normal
colon tumor GW98-166	21940	15.41	792265.93	1584531.87	colon tumor	3.99
colon normal GW98-178	22080	20.06	9968.38	19936.77	colon normal
colon tumor GW98-177	22060	16.81	187186.80	374373.59	colon tumor	18.78
colon normal GW98-561	23514	17.67	81798.86	163597.71	colon normal
colon tumor GW98-560	23513	17.23	124293.97	248587.94	colon tumor	1.52
colon normal GW98-894	24691	16.49	257495.16	514990.32	colon normal
colon tumor GW98-893	24690	15.72	569314.61	1138629.22	colon tumor	2.21
lung normal GW98-3	20742	16.06	399175.12	798350.24	lung normal
lung tumor GW98-2	20741	18.57	35873.21	71746.43	lung tumor	−11.13
lung normal GW97-179	20677	16.42	276326.62	552653.24	lung normal
lung tumor GW97-178	20676	16.05	403321.76	806643.52	lung tumor	1.46
lung normal GW98-165	21922	16.23	335188.32	670376.65	lung normal
lung tumor GW98-164	21921	15.81	517863.70	1035727.40	lung tumor	1.54
lung normal GW98-282	22584	17.35	110774.20	221548.39	lung normal
lung tumor GW98-281	22583	16.81	187186.80	374373.59	lung tumor	1.69
breast normal GW00-392	28750	18.05	57468.10	57468.10	breast normal
breast tumor GW00-391	28746	18.1	54890.07	109780.14	breast tumor	1.91
breast normal GW00-413	28798	17.68	81034.58	81034.58	breast normal
breast tumor GW00-412	28797	17.16	132979.44	265958.88	breast tumor	3.28
breast normal GW00-	27592-95	18.28	46578.77	46578.77	breast normal
235:238
breast tumor GW00-	27588-91	17.38	107643.99	107643.99	breast tumor	2.31
231:234
breast normal GW98-621	23656	17.7	79528.66	159057.32	breast normal
breast tumor GW98-620	23655	15.92	461587.39	923174.78	breast tumor	5.80
brain normal BB99-542	25507	16.61	228308.17	456616.33	brain normal
brain normal BB99-406	25509	17.03	150862.61	301725.22	brain normal
brain normal BB99-904	25546	17.06	146520.74	293041.47	brain normal
brain stage 5 ALZ BB99-	25502	18.71	31670.09	63340.18	brain stage 5	−5.53
874					ALZ
brain stage 5 ALZ BB99-	25503	17.06	146520.74	293041.47	brain stage 5	−1.20
887					ALZ
brain stage 5 ALZ BB99-	25504	18.11	54389.34	108778.68	brain stage 5	−3.22
862					ALZ
brain stage 5 ALZ BB99-	25542	17.26	120757.64	241515.28	brain stage 5	−1.45
927					ALZ
CT lung	normal	16.86	178186.17	356372.33	CT lung Nml
lung 26	normal	18.57	35873.21		lung 26 Nml
lung 27	normal	18.73	31113.64	31113.64	lung 27 Nml
lung 24	COPD	19.35	18125.41	18125.41	lung 24 COPD	−8.20
lung 28	COPD	18.58	35554.21	35554.21	lung 28 COPD	−4.18
lung 23	COPD	19.47	16358.32	16358.32	lung 23 COPD	−9.09
lung 25	normal	18.03	58534.98	58534.98	lung 25 Nml
asthmatic lung ODO3112	29321	17.08	143699.90	143699.90	asthmatic lung	−1.03
asthmatic lung ODO3433	29323	16.87	176441.75	352883.50	asthmatic lung	2.37
asthmatic lung ODO3397	29322	15.84	501828.37	1003656.73	asthmatic lung	6.75
asthmatic lung ODO4928	29325	16.58	235259.88	470519.77	asthmatic lung	3.16
endo cells	control	17.3	116207.60	116207.60	endo cells
endo VEGF		17.49	96945.41	96945.41	endo VEGF	−1.20
endo bFGF		18.04	57998.94	57998.94	endo bFGF	−2.00
heart Clontech	normal	19.29	19083.85	38167.71	heart
heart (T-1) ischemic	29417	17.76	75186.28	150372.55	heart (T-1)	3.94
					ischemic
heart (T-14) non-	29422	17.85	69137.71	138275.42	heart (T-14) non-	3.62
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	17.53	93339.68	186679.35	heart (T-3399)	4.89
					DCM
adenoid GW99-269	26162	16.25	328408.78	656817.56	adenoid
tonsil GW98-280	22582	15.72	569314.61	1138629.22	tonsil
T cells PC00314	28453	16.39	284839.38	569678.76	T cells
PBMNC		18.07	56421.81	56421.81	PBMNC
monocyte		17.81	71759.68	143519.37	monocyte
B cells PC00665	28455	17.27	119602.70	239205.39	B cells
dendritic cells	28441	15.44	767107.95	1534215.89	dendritic cells
neutrophils	28440	15.1	1109957.39	1109957.39	neutrophils
eosinophils	28446	16.12	375225.96	750451.92	eosinophils
BM unstim		17.12	138229.52	138229.52	BM unstim
BM stim	treated	16.1	383035.13	383035.13	BM stim	2.77
osteo dif	treated	16.95	163121.51	163121.51	osteo dif	2.79
osteo undif		18.03	58534.98	58534.98	osteo undif
chondrocytes		16.89	173007.01	432517.52	chondrocytes
OA Synovium IP12/01	29462	16.23	335188.32	335188.32	OA Synovium
OA Synovium NP10/01	29461	17.62	85736.55	171473.11	OA Synovium
OA Synovium NP57/00	28464	16.24	331780.19	663560.38	OA Synovium
RA Synovium	28466	15.7	581468.02	1162936.04	RA Synovium
NP03/01
RA Synovium	28467	16.3	312088.06	624176.11	RA Synovium
NP71/00
RA Synovium	28475	16.02	416038.02	832076.03	RA Synovium
NP45/00
OA bone (biobank)	29217	17.28	118459.46	118459.46	OA bone
					(biobank)
OA bone Sample 1	J. Emory	16.52	249845.54	499691.09	OA bone
OA bone Sample 2	J. Emory	16.65	219376.15	438752.31	OA bone
Cartilage (pool)	Normal	17.02	152340.05	304680.10	Nml Cartilage
					(pool)
Cartilage (pool)	OA	17.55	91590.38	183180.77	OA Cartilage	−1.66
					(pool)
PBL unifected	28441	14.25	2902645.67	5805291.34	PBL unifected
PBL HIV IIIB	28442	15.12	1085846.74	2171693.49	PBL HIV IIIB	−2.67
MRC5 uninfected (100%)	29158	16.31	308928.36	617856.71	MRC5
					uninfected
					(100%)
MRC5 HSV strain F	29178	22.02	2122.64	4245.29	MRC5 HSV	−145.54
					strain F
W12 cells	29179	17.46	99746.51	199493.02	W12 cells	−1.28
Keratinocytes	29180	17.2	127940.29	255880.58	Keratinocytes
B-actin control		25.54	181.26
genomic		23	1030.66	Copy number was calculated from the
				standard curve run on the normal plate
1.00E+05
1.00E+05
1.00E+04
1.00E+04
1.00E+03
1.00E+03
1.00E+02
1.00E+02
1.00E+01
1.00E+01
1.00E+00
1.00E+00
NTC		40	0.11
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0199] Gene Name sbg1551159thymosinb4

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      3.99
colon tumor                      18.78
colon tumor                      1.52
colon tumor                      2.21
lung tumor                       −11.13
lung tumor                       1.46
lung tumor                       1.54
lung tumor                       1.69
breast tumor                     1.91
breast tumor                     3.28
breast tumor                     2.31
breast tumor                     5.80
brain stage 5 ALZ                −5.53
brain stage 5 ALZ                −1.20
brain stage 5 ALZ                −3.22
brain stage 5 ALZ                −1.45
lung 24 COPD                     −8.20
lung 28 COPD                     −4.18
lung 23 COPD                     −9.09
asthmatic lung                   −1.03
asthmatic lung                   2.37
asthmatic lung                   6.75
asthmatic lung                   3.16
endo VEGF                        −1.20
endo bFGF                        −2.00
heart (T-1) ischemic             3.94
heart (T-14) non-obstructive DCM 3.62
heart (T-3399) DCM               4.89
BM stim                          2.77
osteo dif                        2.79
OA Cartilage (pool)              −1.66
PBL HIV IIIB                     −2.67
MRC5 HSV strain F                −145.54
W12 cells                        −1.28

[0200] Gene Name SBh1686954.SOCS

[0201] Moderate to high overall expression. The highest disease expression is seen in the whole brain, fetal liver, and thymus. The expression of this gene in the disease samples is fairly ubiquitous with the highest expression seen in the uninfected PBLs. Upregulation in 1 of 4 breast tumor samples implicates this gene in cancer of the breast Upregulation in 2 of 4 asthmatic lung samples with corroborating high expression in the eosinophils and neutrophils indicates a possible involvement in asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulated in the stimulated bone marrow. Downregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in T cells, B cells, dendritic cells, eosinophils, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
SBh1686954.SOCS	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	32.3, 32.23	71.17	73.85	72.51	3.06	16.34	1184.80
Adipocyt s Zenbio
Subcutaneous	40, 35.35	0	11.59	5.80	0.96	52.36	303.40
Adipose Zenbio
Adrenal Gland	36.03, 40	7.75	0	3.88	0.61	81.97	317.62
Clontech
Whole Brain Clontech	23.81, 24.1	10980.32	9261.99	10121.16	7.24	6.91	69897.48
Fetal Brain Clontech	36.67, 36.09	5.3	7.46	6.38	0.48	103.95	663.20
Cerebellum Clontech	32.53, 33.22	62.11	41.03	51.57	2.17	23.04	1188.25
Cervix	33.29, 33.86	39.34	28.13	33.74	2.42	20.66	697.00
Colon	32.35, 32.44	68.76	65.19	66.98	2.71	18.45	1235.70
Endometrium	34.23, 34.76	22.57	16.49	19.53	0.73	68.21	1332.20
Esophagus	33.87, 32.62	27.89	58.8	43.35	1.37	36.50	1581.93
Heart Clontech	33.96, 34.93	26.56	14.88	20.72	1.32	37.88	784.85
Hypothalamus	35.05, 40	13.88	0	6.94	0.32	155.28	1077.64
Ileum	33.1, 32.27	44.29	72.1	58.20	2.58	19.38	1127.81
Jejunum	30.33, 30.78	228.25	174.81	201.53	6.60	7.58	1526.74
Kidney	34.57, 32.85	18.49	51.19	34.84	2.12	23.58	821.70
Liver	34.31, 35.6	21.5	9.98	15.74	1.50	33.33	524.67
Fetal Liver Clontech	27.29, 27.16	1395.02	1500.44	1447.73	10.40	4.81	6960.24
Lung	32.91, 32.27	49.38	72.19	60.79	2.57	19.46	1182.59
Mammary Gland	29.07, 28.86	483.61	546.94	515.28	13.00	3.85	1981.83
Clontech
Myometrium	32.22, 31.71	74.6	100.64	87.62	2.34	21.37	1872.22
Omentum	32.63, 32.61	58.22	58.94	58.58	3.94	12.69	743.40
Ovary	31.47, 32.28	116.33	71.94	94.14	4.34	11.52	1084.50
Pancreas	36.97, 34.24	4.43	22.51	13.47	0.81	61.80	832.51
Head of Pancreas	35.86, 35.9	8.58	8.37	8.48	1.57	31.85	269.90
Parotid Gland	33.32, 32.02	38.8	83.82	61.31	5.48	9.12	559.40
Placenta Clontech	32.27, 31.3	72.4	128.39	100.40	5.26	9.51	954.33
Prostate	32.41, 33.27	66.44	40.02	53.23	3.00	16.67	887.17
Rectum	32.22, 31.7	74.56	101.26	87.91	1.23	40.65	3573.58
Salivary Gland	31.41, 31.63	120.65	105.6	113.13	7.31	6.84	773.77
Clontech
Skeletal Muscle	36.03, 40	7.74	0.15	3.95	1.26	39.68	156.55
Clontech
Skin	34.93, 35.01	14.9	14.2	14.55	1.21	41.32	601.24
Small Intestine	40, 39.62	3	0.92	1.96	0.98	51.07	100.10
Clontech
Spleen	31.69, 31.36	102.03	124.35	113.19	4.92	10.16	1150.30
Stomach	33.11, 31.62	43.83	106.19	75.01	2.73	18.32	1373.81
Testis Clontech	33.57, 34.29	33.4	21.76	27.58	0.57	87.87	2423.55
Thymus Clontech	25.01, 24.89	5395.44	5795.91	5595.68	9.89	5.06	28289.56
Thyroid	33.46, 33.09	35.61	44.36	39.99	2.77	18.05	721.75
Trachea Clontech	31.52, 31.18	112.93	138.06	125.50	9.71	5.15	646.22
Urinary Bladder	30.13, 29.48	258.39	378.54	318.47	5.47	9.14	2911.01
Uterus	31.08, 31.12	147.02	143.19	145.11	5.34	9.36	1358.66
genomic	27.15	1510.74
b-actin	28.33	750.86
1.00E+05	20.48	100000
1.00E+05	20.41	100000
1.00E+04	23.74	10000
1.00E+04	23.67	10000
1.00E+03	27.33	1000
1.00E+03	27.12	1000
1.00E+02	33.04	100
1.00E+02	31.66	100
1.00E+01	35.21	10
1.00E+01	35.81	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	−1
NTC	40	0
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
SBh1686954.SOCS	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	25.21	5620.96	11241.92	colon normal
colon tumor GW98-166	21940	25.47	4871.05	9742.10	colon tumor	−1.15
colon normal GW98-178	22080	27.8	1370.8	2741.60	colon normal
colon tumor GW98-177	22060	25.49	4833.72	9667.44	colon tumor	3.53
colon normal GW98-561	23514	26.34	3033.87	6067.74	colon normal
colon tumor GW98-560	23513	26.18	3313.57	6627.14	colon tumor	1.09
colon normal GW98-894	24691	24.96	6438.53	12877.06	colon normal
colon tumor GW98-893	24690	25.12	5911.83	11823.66	colon tumor	−1.09
lung normal GW98-3	20742	25.36	5173.46	10346.92	lung normal
lung tumor GW98-2	20741	26.06	3540	7080.00	lung tumor	−1.46
lung normal GW97-179	20677	24.02	10757.4	21514.80	lung normal
lung tumor GW97-178	20676	25.27	5443.34	10886.68	lung tumor	−1.98
lung normal GW98-165	21922	25.16	5771.16	11542.32	lung normal
lung tumor GW98-164	21921	25.38	5122.16	10244.32	lung tumor	−1.13
lung normal GW98-282	22584	25.2	5659.86	11319.72	lung normal
lung tumor GW98-281	22583	26.67	2543.14	5086.28	lung tumor	−2.23
breast normal GW00-392	28750	26.49	2804.16	2804.16	breast normal
breast tumor GW00-391	28746	26.22	3249.93	6499.86	breast tumor	2.32
breast normal GW00-413	28798	28.07	1183.39	1183.39	breast normal
breast tumor GW00-412	28797	25.76	4176.56	8353.12	breast tumor	7.06
breast normal GW00-	27592-95	28.35	1014.62	1014.62	breast normal
235:238
breast tumor GW00-	27588-91	26.06	3531.95	3531.95	breast tumor	3.48
231:234
breast normal GW98-621	23656	25.4	5077.73	10155.46	breast normal
breast tumor GW98-620	23655	25.87	3922.14	7844.28	breast tumor	−1.29
brain normal BB99-542	25507	25.15	5812.78	11625.56	brain normal
brain normal BB99-406	25509	25.82	4043.66	8087.32	brain normal
brain normal BB99-904	25546	26.05	3549.95	7099.90	brain normal
brain stage 5	25502	26.16	3351.06	6702.12	brain stage	−1.33
ALZ BB99-874					5 ALZ
brain stage 5	25503	24.09	10322.56	20645.12	brain stage	2.31
ALZ BB99-887					5 ALZ
brain stage 5	25504	24.46	8438.18	16876.36	brain stage	1.89
ALZ BB99-862					5 ALZ
brain stage 5	25542	24.81	7008.05	14016.10	brain stage	1.57
ALZ BB99-927					5 ALZ
CT lung	normal	27.44	1667.22	3334.44	CT lung Nml
lung 26	normal	30.89	254.94		lung 26 Nml
lung 27	normal	31.43	189.67	189.67	lung 27 Nml
lung 24	COPD	31.8	155.1	155.10	lung 24 COPD	−8.09
lung 28	COPD	30.1	392.91	392.91	lung 28 COPD	−3.19
lung 23	COPD	31.25	209.08	209.08	lung 23 COPD	−6.00
lung 25	normal	31	240.09	240.09	lung 25 Nml
asthmatic lung ODO3112	29321	27.63	1504.34	1504.34	asthmatic lung	1.20
asthmatic lung ODO3433	29323	26.88	2262.28	4524.56	asthmatic lung	3.61
asthmatic lung ODO3397	29322	25.01	6274.02	12548.04	asthmatic lung	10.00
asthmatic lung ODO4928	29325	26.38	2978.98	5957.96	asthmatic lung	4.75
endo cells	control	29.16	654.47	654.47	endo cells
endo VEGF		29.35	589.28	589.28	endo VEGF	−1.11
endo bFGF		29.29	608.94	608.94	endo bFGF	−1.07
heart Clontech	normal	31.92	145.7	291.40	heart
heart (T-1) ischemic	29417	25.47	4876.38	9752.76	heart (T-1)	33.47
					ischemic
heart (T-14) non-	29422	25.27	5449.4	10898.80	heart (T-14) non-	37.40
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	24.97	6405.26	12810.52	heart (T-3399)	43.96
					DCM
adenoid GW99-269	26162	26.65	2571.85	5143.70	adenoid
tonsil GW98-280	22582	24.76	7176.4	14352.80	tonsil
T cells PC00314	28453	24.46	8477.94	16955.88	T cells
PBMNC		31.29	204.63	204.63	PBMNC
monocyte		32.64	98.31	196.62	monocyte
B cells PC00665	28455	25.27	5432.39	10864.78	B cells
dendritic cells	28441	25.34	5235.78	10471.56	dendritic cells
neutrophils	28440	26.4	2934.13	2934.13	neutrophils
eosinophils	28446	27.63	1501.86	3003.72	eosinophils
BM unstim		31.26	208.54	208.54	BM unstim
BM stim	treated	28.19	1107.88	1107.88	BM stim	5.31
osteo dif	treated	28.42	978.61	978.61	osteo dif	2.72
osteo undif		30.26	359.85	359.85	osteo undif
chondrocytes		25.79	4101.89	10254.73	chondrocytes
OA Synovium IP12/01	29462	25.83	4018.19	4018.19	OA Synovium
OA Synovium NP10/01	29461	27.08	2028.25	4056.50	OA Synovium
OA Synovium NP57/00	28464	26.04	3586.44	7172.88	OA Synovium
RA Synovium	28466	25.23	5575.78	11151.56	RA Synovium
NP03/01
RA Synovium	28467	25.5	4801.07	9602.14	RA Synovium
NP71/00
RA Synovium	28475	25.28	5411.66	10823.32	RA Synovium
NP45/00
OA bone (biobank)	29217	27.78	1384.45	1384.45	OA bone (biobank)
OA bone Sample 1	J. Emory	26.36	3001.78	6003.56	OA bone
OA bone Sample 2	J. Emory	26.33	3061.4	6122.80	OA bone
Cartilage (pool)	Normal	26.08	3495.6	6991.20	Nml Cartilage
					(pool)
Cartilage (pool)	OA	27.28	1817.93	3635.86	OA Cartilage	−1.92
					(pool)
PBL unifected	28441	23.28	16055.84	32111.68	PBL unifected
PBL HIV IIIB	28442	24.79	7050.65	14101.30	PBL HIV IIIB	−2.28
MRC5 uninfected	29158	24.72	7336.23	14672.46	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	29.64	502.36	1004.72	MRC5 HSV strain F	−14.60
W12 cells	29179	25.13	5884.35	11768.70	W12 cells	−1.36
Keratinocytes	29180	24.56	8015.6	16031.20	Keratinocytes
B-actin control		27.84	1345.71
genomic		27.48	1632.59
1.00E+05		20.4	100000
1.00E+05		20.45	100000
1.00E+04		24.08	10000
1.00E+04		24.06	10000
1.00E+03		27.51	1000
1.00E+03		27.75	1000
1.00E+02		32.65	100
1.00E+02		32.07	100
1.00E+01		39.7	10
1.00E+01		35.14	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0202] Gene Name SBh1686954.SOCS

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.15
colon tumor                      3.53
colon tumor                      1.09
colon tumor                      −1.09
lung tumor                       −1.46
lung tumor                       −1.98
lung tumor                       −1.13
lung tumor                       −2.23
breast tumor                     2.32
breast tumor                     7.06
breast tumor                     3.48
breast tumor                     −1.29
brain stage 5 ALZ                −1.33
brain stage 5 ALZ                2.31
brain stage 5 ALZ                1.89
brain stage 5 ALZ                1.57
lung 24 COPD                     −8.09
lung 28 COPD                     −3.19
lung 23 COPD                     −6.00
asthmatic lung                   1.20
asthmatic lung                   3.61
asthmatic lung                   10.00
asthmatic lung                   4.75
endo VEGF                        −1.11
endo bFGF                        −1.07
heart (T-1) ischemic             33.47
heart (T-14) non-obstructive DCM 37.40
heart (T-3399) DCM               43.96
BM stim                          5.31
osteo dif                        2.72
OA Cartilage (pool)              −1.92
PBL HIV IIIB                     −2.28
MRC5 HSV strain F                −14.60
W12 cells                        −1.36

[0203] Gene Name sbg1548844slit

[0204] Moderate overall expression. The highest normal expression is seen in the whole brain, cerebellum, and thymus. The expression in the disease samples is highly specific to most of the immune cells including the adenoid, tonsil, T cells, B cells, dendritic cells, neutrophils, eosinophils, and the uninfected peripheral blood lymphocytes. Upregulation in 3 of 4 asthmatic lung samples with corroborating high expression in the immune cells indicates a possible involvement in asthma. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV. High expression in the RA synovium samples with corroborating high immune cell expression implicates this gene in rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
Sample	Ct	copies	copies	Average	18 S	18 S	50 ng
sbg1548844-	(sample	(sample	(sample	GOI	rRNA	rRNA	total
slit	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	35.66, 35.22	13.12	17.14	15.13	3.06	16.34	247.22
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	1.15	0.58	0.61	81.97	47.13
Whole Brain Clontech	24.5, 24.47	11183.13	11425.02	11304.08	7.24	6.91	78066.82
Fetal Brain Clontech	40, 34.17	0	32.33	16.17	0.48	103.95	1680.35
Cerebellum Clontech	30.27, 30.05	341.45	390.51	365.98	2.17	23.04	8432.72
Cervix	37.28, 35.24	4.93	16.91	10.92	2.42	20.66	225.62
Colon	34.68, 34.59	23.72	25.1	24.41	2.71	18.45	450.37
Endometrium	35.92, 40	11.2	0	5.60	0.73	68.21	381.99
Esophagus	36.08, 34.63	10.14	24.48	17.31	1.37	36.50	631.75
Heart Clontech	40, 39.79	0	1.08	0.54	1.32	37.88	20.45
Hypothalamus	36.2, 35.94	9.48	11.06	10.27	0.32	155.28	1594.72
Ileum	35.06, 32.36	18.85	96.29	57.57	2.58	19.38	1115.70
Jejunum	32.51, 33.11	87.93	61.21	74.57	6.60	7.58	564.92
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	40, 34.67	0.58	23.92	12.25	1.50	33.33	408.33
Fetal Liver Clontech	33.24, 32.48	56.8	89.69	73.25	10.40	4.81	352.14
Lung	34.9, 34.3	20.72	29.76	25.24	2.57	19.46	491.05
Mammary Gland	33.77, 32.06	41.15	115.89	78.52	13.00	3.85	302.00
Clontech
Myometrium	34.19, 32.29	31.86	100.92	66.39	2.34	21.37	1418.59
Omentum	36.99, 36.81	5.85	6.54	6.20	3.94	12.69	78.62
Ovary	36.31, 35.43	8.83	15.02	11.93	4.34	11.52	137.38
Pancreas	40, 40	0	0.48	0.24	0.81	61.80	14.83
Head of Pancreas	40, 40	0.66	0	0.33	1.57	31.85	10.51
Parotid Gland	34.63, 34.72	24.44	23.13	23.79	5.48	9.12	217.02
Placenta Clontech	35.8, 34.44	12.06	27.49	19.78	5.26	9.51	187.98
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	36.18, 35.32	9.59	16.14	12.87	1.23	40.65	522.97
Salivary Gland Clontech	33.94, 33.93	37.04	37.34	37.19	7.31	6.84	254.38
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 36.59	0.56	7.48	4.02	1.21	41.32	166.12
Small Intestine Clontech	38.3, 38.52	2.65	2.33	2.49	0.98	51.07	127.17
Spleen	32.12, 33.2	111.43	57.88	84.66	4.92	10.16	860.32
Stomach	36.39, 36.57	8.44	7.56	8.00	2.73	18.32	146.52
Testis Clontech	36.46, 35.95	8.09	10.97	9.53	0.57	87.87	837.43
Thymus Clontech	29.65, 29.31	496.27	611.35	553.81	9.89	5.06	2799.85
Thyroid	35.46, 40	14.78	0.58	7.68	2.77	18.05	138.63
Trachea Clontech	33.19, 33.11	58.51	61.4	59.96	9.71	5.15	308.73
Urinary Bladder	34.52, 40	26.07	0.49	13.28	5.47	9.14	121.39
Uterus	35.15, 34.22	17.85	31.25	24.55	5.34	9.36	229.87
genomic	28.36	1082.56
b-actin	27.98	1361.09
1.00E+05	20.83	100000
1.00E+05	21.28	100000
1.00E+04	24.41	10000
1.00E+04	24.5	10000
1.00E+03	28.3	1000
1.00E+03	28.59	1000
1.00E+02	32.78	100
1.00E+02	32.02	100
1.00E+01	36.15	10
1.00E+01	36.09	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1548844-	(GSK		GOI	total		Disease
slit	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	28.08	1161.39	2322.78	colon normal
colon tumor GW98-166	21940	28.55	883.23	1766.46	colon tumor	−1.31
colon normal GW98-178	22080	29.89	408.18	816.36	colon normal
colon tumor GW98-177	22060	31.89	129.04	258.08	colon tumor	−3.16
colon normal GW98-561	23514	28.25	1047.87	2095.74	colon normal
colon tumor GW98-560	23513	30.26	330.61	661.22	colon tumor	−3.17
colon normal GW98-894	24691	30.06	369	738.00	colon normal
colon tumor GW98-893	24690	30.38	307.17	614.34	colon tumor	−1.20
lung normal GW98-3	20742	28.22	1069.03	2138.06	lung normal
lung tumor GW98-2	20741	31.95	124.8	249.60	lung tumor	−8.57
lung normal GW97-179	20677	26.31	3214.71	6429.42	lung normal
lung tumor GW97-178	20676	28.73	797.3	1594.60	lung tumor	−4.03
lung normal GW98-165	21922	28.38	976.74	1953.48	lung normal
lung tumor GW98-164	21921	27.99	1219.61	2439.22	lung tumor	1.25
lung normal GW98-282	22584	28.53	892.32	1784.64	lung normal
lung tumor GW98-281	22583	29.35	557.89	1115.78	lung tumor	−1.60
breast normal GW00-392	28750	29.91	403.21	403.21	breast normal
breast tumor GW00-391	28746	29.9	405.69	811.38	breast tumor	2.01
breast normal GW00-413	28798	29.39	545.81	545.81	breast normal
breast tumor GW00-412	28797	29.84	419.06	838.12	breast tumor	1.54
breast normal GW00-	27592-95	29.87	412.4	412.40	breast normal
235:238
breast tumor GW00-	27588-91	29.51	506.62	506.62	breast tumor	1.23
231:234
breast normal GW98-621	23656	29.1	643.06	1286.12	breast normal
breast tumor GW98-620	23655	29.49	514.85	1029.70	breast tumor	−1.25
brain normal BB99-542	25507	27.37	1746.53	3493.06	brain normal
brain normal BB99-406	25509	28.07	1165	2330.00	brain normal
brain normal BB99-904	25546	27.12	2019.32	4038.64	brain normal
brain stage 5 ALZ BB99-	25502	28.97	695.1	1390.20	brain stage 5 ALZ	−2.36
874
brain stage 5 ALZ BB99-	25503	27.22	1896.94	3793.88	brain stage 5 ALZ	1.15
887
brain stage 5 ALZ BB99-	25504	27.47	1645.13	3290.26	brain stage 5 ALZ	1.00
862
brain stage 5 ALZ BB99-	25542	28	1212.32	2424.64	brain stage 5 ALZ	−1.36
927
CT lung	normal	28.55	882.77	1765.54	CT lung Nml
lung 26	normal	29.86	415.01		lung 26 Nml
lung 27	normal	35.3	18.03	18.03	lung 27 Nml
lung 24	COPD	33.39	54.36	54.36	lung 24 COPD	−11.28
lung 28	COPD	33.35	55.57	55.57	lung 28 COPD	−11.03
lung 23	COPD	31.82	134.44	134.44	lung 23 COPD	−4.56
lung 25	normal	33.35	55.41	55.41	lung 25 Nml
asthmatic lung ODO3112	29321	28.8	765.75	765.75	asthmatic lung	1.25
asthmatic lung ODO3433	29323	28.19	1090.08	2180.16	asthmatic lung	3.56
asthmatic lung ODO3397	29322	27.26	1854.74	3709.48	asthmatic lung	6.05
asthmatic lung ODO4928	29325	27.68	1457.04	2914.08	asthmatic lung	4.75
endo cells	control	34.77	24.44	24.44	endo cells
endo VEGF		40	0	0.00	endo VEGF	−24.44
endo bFGF		40	0	0.00	endo bFGF	−24.44
heart Clontech	normal	29.86	415.24	830.48	heart
heart (T-1) ischemic	29417	33.68	45.87	91.74	heart (T-1)	−9.05
					ischemic
heart (T-14) non-	29422	31.26	185.72	371.44	heart (T-14) non-	−2.24
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	32.44	93.97	187.94	heart (T-3399)	−4.42
					DCM
adenoid GW99-269	26162	26.91	2268.51	4537.02	adenoid
tonsil GW98-280	22582	25.76	4411.66	8823.32	tonsil
T cells PC00314	28453	25.29	5793.97	11587.94	T cells
PBMNC		31.07	206.88	206.88	PBMNC
monocyte		29.66	465.72	931.44	monocyte
B cells PC00665	28455	24.15	11177.51	22355.02	B cells
dendritic cells	28441	26.5	2884.61	5769.22	dendritic cells
neutrophils	28440	22.68	26075.1	26075.10	neutrophils
eosinophils	28446	24.35	9936.83	19873.66	eosinophils
BM unstim		28.38	972.87	972.87	BM unstim
BM stim	treated	27.74	1410.02	1410.02	BM stim	1.45
osteo dif	treated	36:43	9.41	9.41	osteo dif	1.53
osteo undif		37.17	6.15	6.15	osteo undif
chondrocytes		34.83	23.73	59.33	chondrocytes
OA Synovium IP12/01	29462	28.04	1187.43	1187.43	OA Synovium
OA Synovium NP10/01	29461	30.04	373.5	747.00	OA Synovium
OA Synovium NP57/00	28464	28.11	1137.32	2274.64	OA Synovium
RA Synovium	28466	28.05	1181.53	2363.06	RA Synovium
NP03/01
RA Synovium	28467	28.36	985.37	1970.74	RA Synovium
NP71/00
RA Synovium	28475	28.04	1182.63	2365.26	RA Synovium
NP45/00
OA bone (biobank)	29217	26.12	3584.95	3584.95	OA bone
					(biobank)
OA bone Sample 1	J. Emory	30.9	228.59	457.18	OA bone
OA bone Sample 2	J. Emory	26.59	2736.87	5473.74	OA bone
Cartilage (pool)	Normal	29.13	633.05	1266.10	Nml Cartilage
					(pool)
Cartilage (pool)	OA	30.3	321.92	643.84	OA Cartilage	−1.97
					(pool)
PBL unifected	28441	25.03	6728.29	13456.58	PBL unifected
PBL HIV IIIB	28442	26.51	2858.29	5716.58	PBL HIV IIIB	−2.35
MRC5 uninfected	29158	36.86	7.36	14.72	MRC5
(100%)					uninfected
					(100%)
MRC5 HSV strain F	29178	30.13	355.37	710.74	MRC5 HSV	48.28
					strain F
W12 cells	29179	35.49	16.22	32.44	W12 cells	33.10
Keratinocytes	29180	40	0.49	0.98	Keratinocytes
B-actin control		27.83	1341.01
genomic		28.49	916.45
1.00E+05		20.6	100000
1.00E+05		20.5	100000
1.00E+04		23.82	10000
1.00E+04		23.85	10000
1.00E+03		28.1	1000
1.00E+03		27.86	1000
1.00E+02		32.6	100
1.00E+02		33.28	100
1.00E+01		36.1	10
1.00E+01		38.23	10
1.00E+00		40	0
1.00E+00		38.73	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0205] Gene Name sbg1548844slit

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.31
colon tumor                      −3.16
colon tumor                      −3.17
colon tumor                      −1.20
lung tumor                       −8.57
lung tumor                       −4.03
lung tumor                       1.25
lung tumor                       −1.60
breast tumor                     2.01
breast tumor                     1.54
breast tumor                     1.23
breast tumor                     −1.25
brain stage 5 ALZ                −2.36
brain stage 5 ALZ                1.15
brain stage 5 ALZ                1.00
brain stage 5 ALZ                −1.36
lung 24 COPD                     −11.28
lung 28 COPD                     −11.03
lung 23 COPD                     −4.56
asthmatic lung                   1.25
asthmatic lung                   3.56
asthmatic lung                   6.05
asthmatic lung                   4.75
endo VEGF                        −24.44
endo bFGF                        −24.44
heart (T-1) ischemic             −9.05
heart (T-14) non-obstructive DCM −2.24
heart (T-3399) DCM               −4.42
BM stim                          1.45
osteo dif                        1.53
OA Cartilage (pool)              −1.97
PBL HIV IIIB                     −2.35
MRC5 HSV strain F                48.28
W12 cells                        33.10

[0206] Gene Name sbg1545249GGT

[0207] Moderate to high overall expression. The highest normal expression is seen in the whole brain, fetal liver, jejunum, and kidney. The highest disease expression is seen in the uninfected PBLs, one of the normal lung samples, two of the lung tumor samples, one of the breast tumor samples, and one of the Alzheimer's brain samples. Upregulation in 1 of 4 asthmatic lung samples with corroborating high expression in the eosinophils and neutrophils indicates a possible involvement in asthma. Upregulated in the stimulated bone marrow and the differentiated osteoblasts. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in T cells, B cells, dendritic cells, eosinophils, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg1545249GGT	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	31.62, 31.21	4.41	5.75	5.08	3.06	16.34	83.01
Adipocytes Zenbio
Subcutaneous	33.61, 34.02	1.22	0.94	1.08	0.96	52.36	56.54
Adipose Zenbio
Adrenal Gland Clontech	34.19, 34.29	0.84	0.79	0.82	0.61	81.97	66.80
Whole Brain Clontech	24.05, 23.77	583.11	695.25	639.18	7.24	6.91	4414.23
Fetal Brain Clontech	32.01, 33.17	3.44	1.63	2.54	0.48	103.95	263.51
Cerebellum Clontech	30.97, 31.22	6.7	5.72	6.21	2.17	23.04	143.09
Cervix	29.82, 29.96	14.13	12.87	13.50	2.42	20.66	278.93
Colon	29.01, 28.36	23.74	36.07	29.91	2.71	18.45	551.75
Endometrium	30.9, 30.51	7.04	9.01	8.03	0.73	68.21	547.41
Esophagus	32.04, 31.44	3.38	4.96	4.17	1.37	36.50	152.19
Heart Clontech	32.72, 32.43	2.17	2.63	2.40	1.32	37.88	90.91
Hypothalamus	35.78, 35.29	0.3	0.42	0.36	0.32	155.28	55.90
Ileum	30.08, 29.55	11.9	16.8	14.35	2.58	19.38	278.10
Jejunum	25.01, 24.73	313.84	375.1	344.47	6.60	7.58	2609.62
Kidney	27.57, 26.71	59.97	104.97	82.47	2.12	23.58	1945.05
Liver	29.26, 29.01	20.27	23.78	22.03	1.50	33.33	734.17
Fetal Liver Clontech	23.26, 23.03	965.92	1122.38	1044.15	10.40	4.81	5019.95
Lung	28.85, 28.96	26.44	24.61	25.53	2.57	19.46	496.60
Mammary Gland	27.08, 26.49	82.59	120.38	101.49	13.00	3.85	390.33
Clontech
Myometrium	28.76, 28.82	27.96	26.89	27.43	2.34	21.37	586.00
Omentum	28.68, 28.19	29.4	40.44	34.92	3.94	12.69	443.15
Ovary	28.57, 29	31.55	23.93	27.74	4.34	11.52	319.59
Pancreas	31.3, 32.5	5.43	2.51	3.97	0.81	61.80	245.36
Head of Pancreas	32.02, 30.57	3.43	8.69	6.06	1.57	31.85	192.99
Parotid Gland	28.88, 28.71	25.9	28.77	27.34	5.48	9.12	249.41
Placenta Clontech	29.76, 29.92	14.66	13.19	13.93	5.26	9.51	132.37
Prostate	27.67, 27.3	56.27	71.35	63.81	3.00	16.67	1063.50
Rectum	30.25, 29.97	10.66	12.85	11.76	1.23	40.65	477.85
Salivary Gland Clontech	28.72, 28.84	28.61	26.59	27.60	7.31	6.84	188.78
Skeletal Muscle	32.84, 33.59	2.01	1.24	1.63	1.26	39.68	64.48
Clontech
Skin	32.24, 32.02	2.97	3.42	3.20	1.21	41.32	132.02
Small Intestine Clontech	32.61, 33.84	2.33	1.06	1.70	0.98	51.07	86.57
Spleen	29.4, 29.09	18.46	22.52	20.49	4.92	10.16	208.23
Stomach	29.3, 28.98	19.69	24.22	21.96	2.73	18.32	402.11
Testis Clontech	29.21, 28.71	20.9	28.86	24.88	0.57	87.87	2186.29
Thymus Clontech	25.72, 25.66	197.6	206.04	201.82	9.89	5.06	1020.32
Thyroid	28.92, 28.47	25.22	33.64	29.43	2.77	18.05	531.23
Trachea Clontech	27.95, 27.51	47.11	62.56	54.84	9.71	5.15	282.36
Urinary Bladder	31.23, 30.92	5.69	6.94	6.32	5.47	9.14	57.72
Uterus	27.58, 27.61	59.69	58.49	59.09	5.34	9.36	553.28
genomic	22.68	1408.8
b-actin	25.39	244.89
1.00E+05	16.6	100000
1.00E+05	16.65	100000
1.00E+04	19.45	10000
1.00E+04	19.5	10000
1.00E+03	22.52	1000
1.00E+03	22.65	1000
1.00E+02	26.79	100
1.00E+02	26.49	100
1.00E+01	30.57	10
1.00E+01	30.13	10
1.00E+00	34.07	1
1.00E+00	34.53	1
NTC	33.14	−1
NTC	34.02	−1
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1545249GGT	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	24.12	568.58	1137.16	colon normal
colon tumor GW98-166	21940	22.66	1447.09	2894.18	colon tumor	2.55
colon normal GW98-178	22080	27.35	72.47	144.94	colon normal
colon tumor GW98-177	22060	26.11	159.63	319.26	colon tumor	2.20
colon normal GW98-561	23514	24.45	461	922.00	colon normal
colon tumor GW98-560	23513	25.46	241.41	482.82	colon tumor	−1.91
colon normal GW98-894	24691	25.3	268.35	536.70	colon normal
colon tumor GW98-893	24690	24.57	426.88	853.76	colon tumor	1.59
lung normal GW98-3	20742	21.23	3603.05	7206.10	lung normal
lung tumor GW98-2	20741	23.74	727.24	1454.48	lung tumor	−4.95
lung normal GW97-179	20677	22.44	1667.66	3335.32	lung normal
lung tumor GW97-178	20676	21.12	3870.78	7741.56	lung tumor	2.32
lung normal GW98-165	21922	21.5	3037.83	6075.66	lung normal
lung tumor GW98-164	21921	23.2	1023.32	2046.64	lung tumor	−2.97
lung normal GW98-282	22584	23.03	1140.35	2280.70	lung normal
lung tumor GW98-281	22583	23.37	918.94	1837.88	lung tumor	−1.24
breast normal GW00-392	28750	24.18	549.13	549.13	breast normal
breast tumor GW00-391	28746	23.86	673.37	1346.74	breast tumor	2.45
breast normal GW00-413	28798	24.69	396.34	396.34	breast normal
breast tumor GW00-412	28797	25	325.41	650.82	breast tumor	1.64
breast normal GW00-	27592-95	24.78	372.82	372.82	breast normal
235:238
breast tumor GW00-	27588-91	24.73	386.53	386.53	breast tumor	1.04
231:234
breast normal GW98-621	23656	23.2	1026.45	2052.90	breast normal
breast tumor GW98-620	23655	21.15	3793.2	7586.40	breast tumor	3.70
brain normal BB99-542	25507	24.88	349.22	698.44	brain normal
brain normal BB99-406	25509	24.34	493.57	987.14	brain normal
brain normal BB99-904	25546	25.81	193.58	387.16	brain normal
brain stage 5 ALZ BB99-	25502	25.38	254.26	508.52	brain stage 5 ALZ	−1.36
874
brain stage 5 ALZ BB99-	25503	24.47	455.16	910.32	brain stage 5 ALZ	1.32
887
brain stage 5 ALZ BB99-	25504	24.84	359.61	719.22	brain stage 5 ALZ	1.04
862
brain stage 5 ALZ BB99-	25542	24.4	474.46	948.92	brain stage 5 ALZ	1.37
927
CT lung	normal	21.68	2706.57	5413.14	CT lung Nml
lung 26	normal	24.34	493.3		lung 26 Nml
lung 27	normal	25.81	193.15	193.15	lung 27 Nml
lung 24	COPD	26.14	156.98	156.98	lung 24 COPD	−12.38
lung 28	COPD	25.92	179.93	179.93	lung 28 COPD	−10.80
lung 23	COPD	27.59	62.3	62.30	lung 23 COPD	−31.20
lung 25	normal	25.57	225.7	225.70	lung 25 Nml
asthmatic lung ODO3112	29321	22.08	2095.01	2095.01	asthmatic lung	1.08
asthmatic lung ODO3433	29323	23.25	989.73	1979.46	asthmatic lung	1.02
asthmatic lung ODO3397	29322	21.19	3692.45	7384.90	asthmatic lung	3.80
asthmatic lung ODO4928	29325	23.47	859.31	1718.62	asthmatic lung	−1.13
endo cells	control	30.71	8.5	8.50	endo cells
endo VEGF		30.51	9.6	9.60	endo VEGF	1.13
endo bFGF		31.09	6.64	6.64	endo bFGF	−1.28
heart Clontech	normal	27.98	48.49	96.98	heart
heart (T-1) ischemic	29417	27.29	75.35	150.70	heart (T-1)	1.55
					ischemic
heart (T-14) non-	29422	28.39	37.3	74.60	heart (T-14) non-	−1.30
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	27.38	71.19	142.38	heart (T-3399)	1.47
					DCM
adenoid GW99-269	26162	26.4	133.14	266.28	adenoid
tonsil GW98-280	22582	24.56	428.49	856.98	tonsil
T cells PC00314	28453	25.39	252.86	505.72	T cells
PBMNC		29.02	24.87	24.87	PBMNC
monocyte		28.26	40.37	80.74	monocyte
B cells PC00665	28455	26.08	162.64	325.28	B cells
dendritic cells	28441	24.48	453.66	907.32	dendritic cells
neutrophils	28440	23.63	775.8	775.80	neutrophils
eosinophils	28446	23.83	682.91	1365.82	eosinophils
BM unstim		27.38	71.14	71.14	BM unstim
BM stim	treated	24.51	443.54	443.54	BM stim	6.23
osteo dif	treated	29.33	20.41	20.41	osteo dif	21.95
osteo undif		34.16	0.93	0.93	osteo undif
chondrocytes		27.1	85.11	212.78	chondrocytes
OA Synovium IP12/01	29462	24.06	590.18	590.18	OA Synovium
OA Synovium NP10/01	29461	26.38	134.76	269.52	OA Synovium
OA Synovium NP57/00	28464	24.58	425.07	850.14	OA Synovium
RA Synovium	28466	25.32	264.96	529.92	RA Synovium
NP03/01
RA Synovium	28467	24.32	500.45	1000.90	RA Synovium
NP71/00
RA Synovium	28475	24.47	454.68	909.36	RA Synovium
NP45/00
OA bone (biobank)	29217	24.82	363.24	363.24	OA bone (biobank)
OA bone Sample 1	J. Emory	24.42	468.77	937.54	OA bone
OA bone Sample 2	J. Emory	25.35	259.37	518.74	OA bone
Cartilage (pool)	Normal	26.17	153.58	307.16	Nml Cartilage
					(pool)
Cartilage (pool)	OA	27.29	75.26	150.52	OA Cartilage (pool)	−2.04
PBL unifected	28441	21.15	3789.69	7579.38	PBL unifected
PBL HIV IIIB	28442	23.03	1144.57	2289.14	PBL HIV IIIB	−3.31
MRC5 uninfected	29158	25.59	222.21	444.42	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	27.31	74.06	148.12	MRC5 HSV strain	−3.00
					F
W12 cells	29179	26.66	112.62	225.24	W12 cells	−2.22
Keratinocytes	29180	25.41	249.47	498.94	Keratinocytes
B-actin control		24.63	410.6
genomic		22.01	2185.62
1.00E+05		16.42	100000
1.00E+05		16.27	100000
1.00E+04		19.39	10000
1.00E+04		19.35	10000
1.00E+03		22.78	1000
1.00E+03		22.61	1000
1.00E+02		26.76	100
1.00E+02		27.24	100
1.00E+01		31.24	10
1.00E+01		31.15	10
1.00E+00		34.14	1
1.00E+00		33.14	1
NTC		36.02	−1
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0208] Gene Name sbg1545249GGT

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      2.55
colon tumor                      2.20
colon tumor                      −1.91
colon tumor                      1.59
lung tumor                       −4.95
lung tumor                       2.32
lung tumor                       −2.97
lung tumor                       −1.24
breast tumor                     2.45
breast tumor                     1.64
breast tumor                     1.04
breast tumor                     3.70
brain stage 5 ALZ                −1.36
brain stage 5 ALZ                1.32
brain stage 5 ALZ                1.04
brain stage 5 ALZ                1.37
lung 24 COPD                     −12.38
lung 28 COPD                     −10.80
lung 23 COPD                     −31.20
asthmatic lung                   1.08
asthmatic lung                   1.02
asthmatic lung                   3.80
asthmatic lung                   −1.13
endo VEGF                        1.13
endo bFGF                        −1.28
heart (T-1) ischemic             1.55
heart (T-14) non-obstructive DCM −1.30
heart (T-3399) DCM               1.47
BM stim                          6.23
osteo dif                        21.95
OA Cartilage (pool)              −2.04
PBL HIV IIIB                     −3.31
MRC5 HSV strain F                −3.00
W12 cells                        −2.22

[0209] Gene Name SBh1813899.Y82017 (Taqman was Performed for this Sample)

[0210] Moderate to low overall expression. The highest normal expression is seen in the omentum, prostate, testis, ovary, and uterus. The highest disease expression is seen in the tonsil sample suggesting that this gene may play a role in chronic ear infections and tonsillitis. Upregulation in 2 of 4 lung tumor samples implicates this gene in cancer of lung. Downregulation in 2 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Downregulation in the VEGF- and bFGF-treated endothelial cells suggests that this gene may be involved in angiogenesis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample	(sampl	(sample	GOI	rRNA	18 S	total
SBh1813899.Y82017	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	37.05, 40	4.96	0	2.48	3.06	16.34	40.52
Adipocytes Zenbio
Subcutaneous	40, 37.67	0	3.35	1.68	0.96	52.36	87.70
Adipose Zenbio
Adrenal Gland Clontech	34.59, 40	23.56	0	11.78	0.61	81.97	965.57
Whole Brain Clontech	34.5, 33.89	24.97	36.63	30.80	7.24	6.91	212.71
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	36.14, 37.22	8.84	4.47	6.66	2.17	23.04	153.34
Cervix	33.95, 33.12	35.31	59.9	47.61	2.42	20.66	983.57
Colon	34.6, 36.17	23.39	8.67	16.03	2.71	18.45	295.76
Endometrium	35, 35.05	18.22	17.67	17.95	0.73	68.21	1224.08
Esophagus	36.54, 35.75	6.84	11.34	9.09	1.37	36.50	331.75
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	35.02, 34.12	18	31.72	24.86	2.58	19.38	481.78
Jejunum	31.06, 30.59	219.67	296.53	258.10	6.60	7.58	1955.30
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	35.07, 33.33	17.44	52.2	34.82	1.50	33.33	1160.67
Fetal Liver Clontech	40, 37.65	0	3.4	1.70	10.40	4.81	8.17
Lung	36.1, 34.93	9.09	18.99	14.04	2.57	19.46	273.15
Mammary Gland	37.41, 37.9	3.95	2.9	3.43	13.00	3.85	13.17
Clontech
Myometrium	36.92, 37.18	5.4	4.57	4.99	2.34	21.37	106.52
Omentum	28.23, 27.82	1321.7	1711.31	1516.51	3.94	12.69	19244.99
Ovary	30.87, 31.14	247.95	208.87	228.41	4.34	11.52	2631.45
Pancreas	37.13, 40	4.72	0	2.36	0.81	61.80	145.86
Head of Pancreas	40, 37.55	0	3.61	1.81	1.57	31.85	57.48
Parotid Gland	31.13, 32.45	210.06	91.6	150.83	5.48	9.12	1376.19
Placenta Clontech	34.44, 35.13	25.95	16.77	21.36	5.26	9.51	203.04
Prostate	28.45, 28.4	1151.04	1187.63	1169.34	3.00	16.67	19488.92
Rectum	38.08, 37.37	2.58	4.06	3.32	1.23	40.65	134.96
Salivary Gland	33.44, 32.39	48.94	94.81	71.88	7.31	6.84	491.62
Clontech
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	35.78, 37.12	11.08	4.74	7.91	1.21	41.32	326.86
Small Intestine	40, 40	0	0	0.00	0.98	51.07	0.00
Clontech
Spleen	33.64, 33.21	43.03	56.35	49.69	4.92	10.16	504.98
Stomach	32.31, 31.4	99.53	178.03	138.78	2.73	18.32	2541.76
Testis Clontech	32.34, 32.33	98.13	98.48	98.31	0.57	87.87	8638.40
Thymus Clontech	37.44, 35.09	3.88	17.16	10.52	9.89	5.06	53.19
Thyroid	35.52, 38.84	13.12	1.6	7.36	2.77	18.05	132.85
Trachea Clontech	30.8, 29.83	259.07	480.34	369.71	9.71	5.15	1903.73
Urinary Bladder	40, 35.78	0	11.08	5.54	5.47	9.14	50.64
Uterus	30.59, 29.63	297.31	545	421.16	5.34	9.36	3943.40
genomic	27.5	2091.83
b-actin	29.13	745.39
1.00E+05	21.9	100000
1.00E+05	21.69	100000
1.00E+04	24.81	10000
1.00E+04	25.02	10000
1.00E+03	28.21	1000
1.00E+03	28.11	1000
1.00E+02	31.91	100
1.00E+02	32.21	100
1.00E+01	35.06	10
1.00E+01	37.77	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
SBh1813899.Y82017	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	38.12	1.03	2.06	colon normal
colon tumor GW98-166	21940	37.49	1.58	3.16	colon tumor	1.53
colon normal GW98-178	22080	35.97	4.43	8.86	colon normal
colon tumor GW98-177	22060	38.1	1.04	2.08	colon tumor	−4.26
colon normal GW98-561	23514	38.75	0.67	1.34	colon normal
colon tumor GW98-560	23513	39.01	0.56	1.12	colon tumor	−1.20
colon normal GW98-894	24691	33.57	22.61	45.22	colon normal
colon tumor GW98-893	24690	36.96	2.25	4.50	colon tumor	−10.05
lung normal GW98-3	20742	38.56	0.76	1.52	lung normal
lung tumor GW98-2	20741	31.65	82.7	165.40	lung tumor	108.82
lung normal GW97-179	20677	34.04	16.4	32.80	lung normal
lung tumor GW97-178	20676	37.01	2.19	4.38	lung tumor	−7.49
lung normal GW98-165	21922	40	0	0.00	lung normal
lung tumor GW98-164	21921	34.02	16.62	33.24	lung tumor	33.24
lung normal GW98-282	22584	31.32	103.46	206.92	lung normal
lung tumor GW98-281	22583	34.18	14.87	29.74	lung tumor	−6.96
breast normal GW00-392	28750	32.79	38.17	38.17	breast normal
breast tumor GW00-391	28746	35.08	8.09	16.18	breast tumor	−2.36
breast normal GW00-413	28798	35.02	8.4	8.40	breast normal
breast tumor GW00-412	28797	34.18	14.89	29.78	breast tumor	3.55
breast normal GW00-	27592-95	32.3	53.22	53.22	breast normal
235:238
breast tumor GW00-	27588-91	36.61	2.87	2.87	breast tumor	−18.54
231:234
breast normal GW98-621	23656	34.23	14.44	28.88	breast normal
breast tumor GW98-620	23655	32.23	56.07	112.14	breast tumor	3.88
brain normal BB99-542	25507	33.42	24.93	49.86	brain normal
brain normal BB99-406	25509	36.23	3.71	7.42	brain normal
brain normal BB99-904	25546	40	0	0.00	brain normal
brain stage 5 ALZ BB99-	25502	40	0	0.00	brain stage 5 ALZ	−19.09
874
brain stage 5 ALZ BB99-	25503	40	0	0.00	brain stage 5 ALZ	−19.09
887
brain stage 5 ALZ BB99-	25504	36.12	4	8.00	brain stage 5 ALZ	−2.39
862
brain stage 5 ALZ BB99-	25542	35.23	7.29	14.58	brain stage 5 ALZ	−1.31
927
CT lung	normal	37.65	1.42	2.84	CT lung Nml
lung 26	normal	36.16	3.88		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	38.06	1.07	1.07	lung 24 COPD	−1.63
lung 28	COPD	35.07	8.13	8.13	lung 28 COPD	4.65
lung 23	COPD	35.22	7.34	7.34	lung 23 COPD	4.20
lung 25	normal	36.87	2.4	2.40	lung 25 Nml
asthmatic lung ODO3112	29321	37.56	1.51	1.51	asthmatic lung	−1.16
asthmatic lung ODO3433	29323	40	0	0.00	asthmatic lung	−1.75
asthmatic lung ODO3397	29322	38.09	1.05	2.10	asthmatic lung	1.20
asthmatic lung ODO4928	29325	40	0	0.00	asthmatic lung	−1.75
endo cells	control	35.81	4.94	4.94	endo cells
endo VEGF		40	0	0.00	endo VEGF	−4.94
endo bFGF		40	0	0.00	endo bFGF	−4.94
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	40	0	0.00	heart (T-1)	0.00
					ischemic
heart (T-14) non-	29422	40	0	0.00	heart (T-14) non-	0.00
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	40	0	0.00	heart (T-3399)	0.00
					DCM
adenoid GW99-269	26162	32.04	63.54	127.08	adenoid
tonsil GW98-280	22582	29.94	264.84	529.68	tonsil
T cells PC00314	28453	40	0	0.00	T cells
PBMNC		37.57	1.49	1.49	PBMNC
monocyte		36.8	2.52	5.04	monocyte
B cells PC00665	28455	32.1	61.2	122.40	B cells
dendritic cells	28441	40	0	0.00	dendritic cells
neutrophils	28440	32.84	36.87	36.87	neutrophils
eosinophils	28446	40	0	0.00	eosinophils
BM unstim		37.22	1.89	1.89	BM unstim
BM stim	treated	36.25	3.65	3.65	BM stim	1.93
osteo dif	treated	40	0	0.00	osteo dif	−1.67
osteo undif		37.41	1.67	1.67	osteo undif
chondrocytes		34.73	10.28	25.70	chondrocytes
OA Synovium IP12/01	29462	35.3	6.98	6.98	OA Synovium
OA Synovium NP10/01	29461	39.02	0.56	1.12	OA Synovium
OA Synovium NP57/00	28464	33.26	27.78	55.56	OA Synovium
RA Synovium	28466	34.81	9.69	19.38	RA Synovium
NP03/01
RA Synovium	28467	35.74	5.18	10.36	RA Synovium
NP71/00
RA Synovium	28475	33.48	23.88	47.76	RA Synovium
NP45/00
OA bone (biobank)	29217	35.34	6.79	6.79	OA bone
					(biobank)
OA bone Sample 1	J. Emory	34.06	16.14	32.28	OA bone
OA bone Sample 2	J. Emory	33.91	17.89	35.78	OA bone
Cartilage (pool)	Normal	40	0	0.00	Nml Cartilage
					(pool)
Cartilage (pool)	OA	40	0	0.00	OA Cartilage	0.00
					(pool)
PBL unifected	28441	36.27	3.6	7.20	PBL unifected
PBL HIV IIIB	28442	35.52	5.99	11.98	PBL HIV IIIB	1.66
MRC5 uninfected	29158	40	0	0.00	MRC5
(100%)					uninfected
					(100%)
MRC5 HSV strain F	29178	37.72	1.35	2.70	MRC5 HSV	2.70
					strain F
W12 cells	29179	40	0	0.00	W12 cells	0.00
Keratinocytes	29180	40	0	0.00	Keratinocytes
B-actin control		29.71	309.67
genomic		28.32	792.66
1.00E+05		21.34	100000
1.00E+05		21.16	100000
1.00E+04		24.15	10000
1.00E+04		24.13	10000
1.00E+03		27.36	1000
1.00E+03		27.69	1000
1.00E+02		33.07	100
1.00E+02		32.04	100
1.00E+01		35.15	10
1.00E+01		35.4	10
1.00E+00		37.34	1
1.00E+00		37.29	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0211] Gene Name SBh1813899.Y82017

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.53
colon tumor                      −4.26
colon tumor                      −1.20
colon tumor                      −10.05
lung tumor                       108.82
lung tumor                       −7.49
lung tumor                       33.24
lung tumor                       −6.96
breast tumor                     −2.36
breast tumor                     3.55
breast tumor                     −18.54
breast tumor                     3.88
brain stage 5 ALZ                −19.09
brain stage 5 ALZ                −19.09
brain stage 5 ALZ                −2.39
brain stage 5 ALZ                −1.31
lung 24 COPD                     −1.63
lung 28 COPD                     4.65
lung 23 COPD                     4.20
asthmatic lung                   −1.16
asthmatic lung                   −1.75
asthmatic lung                   1.20
asthmatic lung                   −1.75
endo VEGF                        −4.94
endo bFGF                        −4.94
heart (T-1) ischemic             0.00
heart (T-14) non-obstructive DCM 0.00
heart (T-3399) DCM               0.00
BM stim                          1.93
osteo dif                        −1.67
OA Cartilage (pool)              0.00
PBL HIV IIIB                     1.66
MRC5 HSV strain F                2.70
W12 cells                        0.00

[0212] Gene Name sbg1536324mapkkk

[0213] Moderate overall expression. The highest normal expression is seen in the whole brain, cerebellum, and kidney as well as many of the tissues representing the GI tract including the colon, jejunum, rectum, and stomach. The highest disease expression is seen in many of the tumor samples and their matched normal counterparts as well as in the uninfected peripheral blood lymphocytes, W12 cells, and the normal keratinocytes. Significant upregulation in 1 of 4 colon tumor samples is sufficient to implicate this gene in colon cancer. Upregulation in 1 of 4 asthmatic lung samples with corroborating high expression in the eosinophils and neutrophils indicates a possible involvement in asthma. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM. Upregulated in the stimulated bone marrow and the differentiated osteoblasts. Downregulation in the HIV-infected PBLs suggests that this gene may be a host factor in HIV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in T cells, B cells, eosinophils, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
Sample	Ct	copies	copies	Average	18 S	50 ng/	50 ng
sbg1536324-	(sample	(sample	(sample	GOI	rRNA	18 S	total
mapkkk	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	35.13, 34.95	8.06	9.01	8.54	3.06	16.34	139.46
Adipocytes Zenbio
Subcutaneous Adipose	40, 36.08	0	4.53	2.27	0.96	52.36	118.59
Zenbio
Adrenal Gland Clontech	35.54, 35.09	6.31	8.3	7.31	0.61	81.97	598.77
Whole Brain Clontech	24.8, 25.05	4253.95	3672.88	3963.42	7.24	6.91	27371.65
Fetal Brain Clontech	36.36, 40	3.83	0.25	2.04	0.48	103.95	212.06
Cerebellum Clontech	30.47, 30.18	136.52	162.55	149.54	2.17	23.04	3445.51
Cervix	33.31, 33.02	24.3	28.99	26.65	2.42	20.66	550.52
Colon	29.65, 30.3	224.17	151.39	187.78	2.71	18.45	3464.58
Endometrium	34.05, 33.15	15.6	26.93	21.27	0.73	68.21	1450.55
Esophagus	34.5, 36.18	11.85	4.28	8.07	1.37	36.50	294.34
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	34.47, 40	12.03	0	6.02	0.32	155.28	934.01
Ileum	32.38, 31.42	42.91	76.52	59.72	2.58	19.38	1157.27
Jejunum	27.49, 27.23	833.68	976.73	905.21	6.60	7.58	6857.61
Kidney	31.02, 29.54	98.02	239.91	168.97	2.12	23.58	3985.02
Liver	33.09, 32.88	27.88	31.55	29.72	1.50	33.33	990.50
Fetal Liver Clontech	30.28, 30.65	153.69	122.23	137.96	10.40	4.81	663.27
Lung	31.61, 31.72	68.47	64.09	66.28	2.57	19.46	1289.49
Mammary Gland	29.5, 28.61	245.39	421.31	333.35	13.00	3.85	1282.12
Clontech
Myometrium	32.34, 31.29	44.01	83	63.51	2.34	21.37	1356.94
Omentum	30.04, 30.7	176.89	118.57	147.73	3.94	12.69	1874.75
Ovary	34.11, 32.17	15.03	48.55	31.79	4.34	11.52	366.24
Pancreas	32.13, 33.42	49.79	22.73	36.26	0.81	61.80	2241.04
Head of Pancreas	32.95, 31.15	30.39	90.67	60.53	1.57	31.85	1927.71
Parotid Gland	29.81, 31.42	204.18	76.85	140.52	5.48	9.12	1282.07
Placenta Clontech	35.22, 34.15	7.65	14.65	11.15	5.26	9.51	105.99
Prostate	32.64, 32.53	36.51	39.11	37.81	3.00	16.67	630.17
Rectum	30.98, 31.06	100.38	95.28	97.83	1.23	40.65	3976.83
Salivary Gland	30.76, 31.26	114.72	84.39	99.56	7.31	6.84	680.95
Clontech
Skeletal Muscle	40, 35.91	0	5.03	2.52	1.26	39.68	99.80
Clontech
Skin	38.91, 35.82	0.82	5.32	3.07	1.21	41.32	126.86
Small Intestine	33.09, 33.17	27.87	26.49	27.18	0.98	51.07	1388.15
Clontech
Spleen	31.76, 33.26	62.51	25.06	43.79	4.92	10.16	444.97
Stomach	30.21, 29.84	160.09	200.01	180.05	2.73	18.32	3297.62
Testis Clontech	35.2, 34.14	7.76	14.71	11.24	0.57	87.87	987.26
Thymus Clontech	29.58, 29.01	234.48	330.52	282.50	9.89	5.06	1428.21
Thyroid	32.1, 32.33	50.8	44.07	47.44	2.77	18.05	856.23
Trachea Clontech	30.02, 29.71	179.68	216.47	198.08	9.71	5.15	1019.95
Urinary Bladder	34.48, 32.46	12.01	40.72	26.37	5.47	9.14	241.00
Uterus	30.3, 30.08	151.8	173.03	162.42	5.34	9.36	1520.74
genomic	27.06	1078.79
b-actin	28.03	601.21
1.00E+05	19.63	100000
1.00E+05	19.9	100000
1.00E+04	23.38	10000
1.00E+04	23.39	10000
1.00E+03	26.84	1000
1.00E+03	27.04	1000
1.00E+02	31.03	100
1.00E+02	30.67	100
1.00E+01	33.53	10
1.00E+01	36.48	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1536324-	(GSK		GOI	total		Disease
mapkkk	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	25.38	3449.66	6899.32	colon normal
colon tumor GW98-166	21940	25.74	2747.28	5494.56	colon tumor	−1.26
colon normal GW98-178	22080	31.04	102.2	204.40	colon normal
colon tumor GW98-177	22060	25.14	4004.98	8009.96	colon tumor	39.19
colon normal GW98-561	23514	25.68	2860.49	5720.98	colon normal
colon tumor GW98-560	23513	25.22	3793.08	7586.16	colon tumor	1.33
colon normal GW98-894	24691	24.74	5130.6	10261.20	colon normal
colon tumor GW98-893	24690	24.03	7978.97	15957.94	colon tumor	1.56
lung normal GW98-3	20742	25.87	2533.92	5067.84	lung normal
lung tumor GW98-2	20741	26.05	2268.34	4536.68	lung tumor	−1.12
lung normal GW97-179	20677	24.92	4568.82	9137.64	lung normal
lung tumor GW97-178	20676	25.15	3982.17	7964.34	lung tumor	−1.15
lung normal GW98-165	21922	25.33	3555.29	7110.58	lung normal
lung tumor GW98-164	21921	25.57	3053.68	6107.36	lung tumor	−1.16
lung normal GW98-282	22584	25.8	2649.48	5298.96	lung normal
lung tumor GW98-281	22583	27.59	872.2	1744.40	lung tumor	−3.04
breast normal GW00-392	28750	28.07	648.67	648.67	breast normal
breast tumor GW00-391	28746	27.22	1096.31	2192.62	breast tumor	3.38
breast normal GW00-413	28798	28.69	440.98	440.98	breast normal
breast tumor GW00-412	28797	28.38	533.68	1067.36	breast tumor	2.42
breast normal GW00-	27592-95	27.2	1109.72	1109.72	breast normal
235:238
breast tumor GW00-	27588-91	28.37	536.17	536.17	breast tumor	−2.07
231:234
breast normal GW98-621	23656	26.19	2085.44	4170.88	breast normal
breast tumor GW98-620	23655	27.31	1037.07	2074.14	breast tumor	−2.01
brain normal BB99-542	25507	25.25	3734.67	7469.34	brain normal
brain normal BB99-406	25509	26.67	1542.92	3085.84	brain normal
brain normal BB99-904	25546	26.4	1829.69	3659.38	brain normal
brain stage 5 ALZ BB99-	25502	28.33	549.79	1099.58	brain stag 5 ALZ	−4.31
874
brain stage 5 ALZ BB99-	25503	26.12	2172.52	4345.04	brain stage 5 ALZ	−1.09
887
brain stage 5 ALZ BB99-	25504	26.39	1840.01	3680.02	brain stage 5 ALZ	−1.29
862
brain stage 5 ALZ BB99-	25542	25.85	2571.02	5142.04	brain stage 5 ALZ	1.09
927
CT lung	normal	27.06	1215.1	2430.20	CT lung Nml
lung 26	normal	28.58	472.09		lung 26 Nml
lung 27	normal	29.77	225.55	225.55	lung 27 Nml
lung 24	COPD	30.61	133.34	133.34	lung 24 COPD	−7.27
lung 28	COPD	30.05	189.33	189.33	lung 28 COPD	−5.12
lung 23	COPD	31.15	95.52	95.52	lung 23 COPD	−10.14
lung 25	normal	29.6	250.5	250.50	lung 25 Nml
asthmatic lung ODO3112	29321	26.96	1285.59	1285.59	asthmatic lung	1.33
asthmatic lung ODO3433	29323	27.15	1145.8	2291.60	asthmatic lung	2.37
asthmatic lung ODO3397	29322	26.02	2314.97	4629.94	asthmatic lung	4.78
asthmatic lung ODO4928	29325	27	1256.03	2512.06	asthmatic lung	2.59
endo cells	control	32.22	49.23	49.23	endo cells
endo VEGF		33.05	29.29	29.29	endo VEGF	−1.68
endo bFGF		32.71	36.25	36.25	endo bFGF	−1.36
heart Clontech	normal	32.81	34.06	68.12	heart
heart (T-1) ischemic	29417	31.63	70.95	141.90	heart (T-1)	2.08
					ischemic
heart (T-14) non-	29422	31.01	104.06	208.12	heart (T-14) non-	3.06
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	29.87	211.37	422.74	heart (T-3399)	6.21
					DCM
adenoid GW99-269	26162	30.24	167.48	334.96	adenoid
tonsil GW98-280	22582	27.83	751.19	1502.38	tonsil
T cells PC00314	28453	30.69	126.89	253.78	T cells
PBMNC		32.29	47.02	47.02	PBMNC
monocyte		32.31	46.34	92.68	monocyte
B cells PC00665	28455	28.55	479.1	958.20	B cells
dendritic cells	28441	27.78	773.27	1546.54	dendritic cells
neutrophils	28440	28.34	546.2	546.20	neutrophils
eosinophils	28446	29.01	361	722.00	eosinophils
BM unstim		31.28	87.86	87.86	BM unstim
BM stim	treated	29.46	273.53	273.53	BM stim	3.11
osteo dif	treated	34.37	12.89	12.89	osteo dif	3.04
osteo undif		36.16	4.24	4.24	osteo undif
chondrocytes		29.72	231.78	579.45	chondrocytes
OA Synovium IP12/01	29462	29.86	212.56	212.56	OA Synovium
OA Synovium NP10/01	29461	31.01	103.92	207.84	OA Synovium
OA Synovium NP57/00	28464	28.77	418.09	836.18	OA Synovium
RA Synovium	28466	28.72	431.53	863.06	RA Synovium
NP03/01
RA Synovium	28467	29.07	347.77	695.54	RA Synovium
NP71/00
RA Synovium	28475	28.56	476.95	953.90	RA Synovium
NP45/00
OA bone (biobank)	29217	29.73	230.13	230.13	OA bone
					(biobank)
OA bone Sample 1	J. Emory	29.35	292.22	584.44	OA bone
OA bone Sample 2	J. Emory	28.82	406.11	812.22	OA bone
Cartilage (pool)	Normal	29.46	272.55	545.10	Nml Cartilage
					(pool)
Cartilage (pool)	OA	30.07	186.61	373.22	OA Cartilage	−1.46
					(pool)
PBL unifected	28441	24.46	6094.2	12188.40	PBL unifected
PBL HIV IIIB	28442	27.78	773.53	1547.06	PBL HIV IIIB	−7.88
MRC5 uninfected	29158	30.08	185.3	370.60	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	31.26	89.14	178.28	MRC5 HSV	−2.08
					strain F
W12 cells	29179	24.9	4626.33	9252.66	W12 cells	1.29
Keratinocytes	29180	25.31	3594.16	7188.32	Keratinocytes
B-actin control		27.42	967.12
genomic		26.69	1523.16
1.00E+05		20.06	100000
1.00E+05		20.16	100000
1.00E+04		23.58	10000
1.00E+04		23.43	10000
1.00E+03		26.98	1000
1.00E+03		27.03	1000
1.00E+02		31.54	100
1.00E+02		31.81	100
1.00E+01		34	10
1.00E+01		35.1	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0214] Gene Name sbg1536324mapkkk

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.26
colon tumor                      39.19
colon tumor                      1.33
colon tumor                      1.56
lung tumor                       −1.12
lung tumor                       −1.15
lung tumor                       −1.16
lung tumor                       −3.04
breast tumor                     3.38
breast tumor                     2.42
breast tumor                     −2.07
breast tumor                     −2.01
brain stage 5 ALZ                −4.31
brain stage 5 ALZ                −1.09
brain stage 5 ALZ                −1.29
brain stage 5 ALZ                1.09
lung 24 COPD                     −7.27
lung 28 COPD                     −5.12
lung 23 COPD                     −10.14
asthmatic lung                   1.33
asthmatic lung                   2.37
asthmatic lung                   4.78
asthmatic lung                   2.59
endo VEGF                        −1.68
endo bFGF                        −1.36
heart (T-1) ischemic             2.08
heart (T-14) non-obstructive DCM 3.06
heart (T-3399) DCM               6.21
BM stim                          3.11
osteo dif                        3.04
OA Cartilage (pool)              −1.46
PBL HIV IIIB                     −7.88
MRC5 HSV strain F                −2.08
W12 cells                        1.29

[0215] Gene Name gsk1810944BrCaAg

[0216] Moderate to low overall expression. The highest normal expression is seen in the pancreas, prostate, and testis. The highest disease expression is seen in the normal and tumor breast samples. Upregulation in 2 of 4 colon tumors, 2 of 4 lung tumors, and 3 of 4 breast tumors implicates this gene in cancers of the colon, lung, and breast. Upregulation in 3 of 4 Alzheimer's brain samples and downregulation in 1 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Downregulated in the stimulated bone marrow. Downregulation in the HIV-infected PBLs suggests that this gene may play a role as a host factor in HIV. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV. Patterns of expression are very similar to gsk1811484BrCaAg.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
Sample	Ct	copies	copies	Average	18 S	50 ng/	50 ng
gsk1810944-	(sample	(sample	(sample	GOI	rRNA	18 S	total
BrCaAg	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	36.25, 36.46	0.79	0.7	0.75	3.06	16.34	12.17
Adipocytes Zenbio
Subcutaneous	40, 40	0	0	0.00	0.96	52.36	0.00
Adipose Zenbio
Adrenal Gland Clontech	40, 38.83	0	0.17	0.09	0.61	81.97	6.97
Whole Brain Clontech	31.12, 31.73	17.51	12.12	14.82	7.24	6.91	102.31
Fetal Brain Clontech	36.67, 40	0.62	0	0.31	0.48	103.95	32.22
Cerebellum Clontech	36.89, 36.74	0.54	0.59	0.57	2.17	23.04	13.02
Cervix	34.39, 33.37	2.45	4.52	3.49	2.42	20.66	72.00
Colon	33.54, 33.99	4.08	3.11	3.60	2.71	18.45	66.33
Endometrium	35.71, 34.14	1.1	2.84	1.97	0.73	68.21	134.38
Esophagus	35.79, 33.75	1.05	3.58	2.32	1.37	36.50	84.49
Heart Clontech	36.93, 40	0.53	0	0.27	1.32	37.88	10.04
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	33.8, 35.28	3.49	1.43	2.46	2.58	19.38	47.67
Jejunum	31.05, 31.01	18.19	18.69	18.44	6.60	7.58	139.70
Kidney	36.21, 36.17	0.82	0.84	0.83	2.12	23.58	19.58
Liver	32.55, 32.09	7.41	9.73	8.57	1.50	33.33	285.67
Fetal Liver Clontech	35.99, 35.95	0.93	0.95	0.94	10.40	4.81	4.52
Lung	35.03, 34.86	1.66	1.83	1.75	2.57	19.46	33.95
Mammary Gland	30.57, 30.66	24.41	23.12	23.77	13.00	3.85	91.40
Clontech
Myometrium	35.15, 36.05	1.54	0.9	1.22	2.34	21.37	26.07
Omentum	30.24, 29.99	29.64	34.55	32.10	3.94	12.69	407.30
Ovary	31.21, 31.39	16.53	14.89	15.71	4.34	11.52	180.99
Pancreas	29.25, 34.15	53.86	2.82	28.34	0.81	61.80	1751.55
Head of Pancreas	34.95, 35.35	1.74	1.37	1.56	1.57	31.85	49.52
Parotid Gland	32.25, 33.2	8.84	5.01	6.93	5.48	9.12	63.18
Placenta Clontech	29.7, 29.86	41.05	37.31	39.18	5.26	9.51	372.43
Prostate	29.4, 29.21	49.3	55.13	52.22	3.00	16.67	870.25
Rectum	37.2, 36.09	0.45	0.87	0.66	1.23	40.65	26.83
Salivary Gland Clontech	33.48, 32.85	4.22	6.18	5.20	7.31	6.84	35.57
Skeletal Muscle Clontech	40, 40	0	0	0.00	1.26	39.68	0.00
Skin	35.73, 34.57	1.09	2.19	1.64	1.21	41.32	67.77
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	32.81, 32.51	6.33	7.56	6.95	4.92	10.16	70.58
Stomach	30.42, 30.07	26.58	32.91	29.75	2.73	18.32	544.78
Testis Clontech	28.85, 28.49	68.5	85.03	76.77	0.57	87.87	6745.61
Thymus Clontech	28.84, 28.44	69.07	87.85	78.46	9.89	5.06	396.66
Thyroid	37.78, 37.22	0.32	0.44	0.38	2.77	18.05	6.86
Trachea Clontech	28.54, 28.05	82.88	111.3	97.09	9.71	5.15	499.95
Urinary Bladder	35.71, 35.32	1.1	1.39	1.25	5.47	9.14	11.38
Uterus	30.33, 30.28	28.15	28.94	28.55	5.34	9.36	267.28
genomic	23.66	1559.65
b-actin	27.75	133.39
1.00E+05	17.56	100000
1.00E+05	17.6	100000
1.00E+04	20.22	10000
1.00E+04	20.22	10000
1.00E+03	23.66	1000
1.00E+03	23.5	1000
1.00E+02	28.44	100
1.00E+02	27.66	100
1.00E+01	32.2	10
1.00E+01	32.08	10
1.00E+00	40	1
1.00E+00	36.72	1
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
gsk1810944-	(GSK		GOI	total		Disease
BrCaAg	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	31.6	16.07	32.14	colon normal
colon tumor GW98-166	21940	26.43	401.48	802.96	colon tumor	24.98
colon normal GW98-178	22080	35.59	1.34	2.68	colon normal
colon tumor GW98-177	22060	34	3.61	7.22	colon tumor	2.69
colon normal GW98-561	23514	34.42	2.79	5.58	colon normal
colon tumor GW98-560	23513	34.98	1.97	3.94	colon tumor	−1.42
colon normal GW98-894	24691	32.07	12.03	24.06	colon normal
colon tumor GW98-893	24690	29.59	56.38	112.76	colon tumor	4.69
lung normal GW98-3	20742	31.62	15.94	31.88	lung normal
lung tumor GW98-2	20741	25.47	731.76	1463.52	lung tumor	45.91
lung normal GW97-179	20677	28.71	97.31	194.62	lung normal
lung tumor GW97-178	20676	31.26	19.96	39.92	lung tumor	−4.88
lung normal GW98-165	21922	31.38	18.43	36.86	lung normal
lung tumor GW98-164	21921	28.19	134.24	268.48	lung tumor	7.28
lung normal GW98-282	22584	30.18	38.92	77.84	lung normal
lung tumor GW98-281	22583	34.33	2.95	5.90	lung tumor	−13.19
breast normal GW00-392	28750	22.69	4112.84	4112.84	breast normal
breast tumor GW00-391	28746	24.07	1742.01	3484.02	breast tumor	−1.18
breast normal GW00-413	28798	25.17	879.08	879.08	breast normal
breast tumor GW00-412	28797	22.83	3790.96	7581.92	breast tumor	8.62
breast normal GW00-	27592-95	27.9	160.94	160.94	breast normal
235:238
breast tumor GW00-	27588-91	22.45	4774.85	4774.85	breast tumor	29.67
231:234
breast normal GW98-621	23656	26.47	392.57	785.14	breast normal
breast tumor GW98-620	23655	24.07	1744.99	3489.98	breast tumor	4.45
brain normal BB99-542	25507	36.64	0.7	1.40	brain normal
brain normal BB99-406	25509	34.99	1.95	3.90	brain normal
brain normal BB99-904	25546	35.36	1.55	3.10	brain normal
brain stage 5 ALZ BB99-	25502	39.15	0.15	0.30	brain stage 5 ALZ	−9.33
874
brain stage 5 ALZ BB99-	25503	32.36	10.05	20.10	brain stage 5 ALZ	7.18
887
brain stage 5 ALZ BB99-	25504	33.44	5.14	10.28	brain stage 5 ALZ	3.67
862
brain stage 5 ALZ BB99-	25542	32.86	7.35	14.70	brain stage 5 ALZ	5.25
927
CT lung	normal	33.56	4.76	9.52	CT lung Nml
lung 26	normal	34.06	3.49		lung 26 Nml
lung 27	normal	37.17	0.5	0.50	lung 27 Nml
lung 24	COPD	36.1	0.98	0.98	lung 24 COPD	−3.56
lung 28	COPD	39.98	0.07	0.07	lung 28 COPD	−49.90
lung 23	COPD	35.14	1.78	1.78	lung 23 COPD	−1.96
lung 25	normal	37.32	0.46	0.46	lung 25 Nml
asthmatic lung ODO3112	29321	36.93	0.58	0.58	asthmatic lung	−6.02
asthmatic lung ODO3433	29323	36.24	0.9	1.80	asthmatic lung	−1.94
asthmatic lung ODO3397	29322	33.42	5.2	10.40	asthmatic lung	2.98
asthmatic lung ODO4928	29325	33.65	4.49	8.98	asthmatic lung	2.57
endo cells	control	40	0	0.00	endo cells
endo VEGF		38.26	0.26	0.26	endo VEGF	0.26
endo bFGF		35.43	1.48	1.48	endo bFGF	1.48
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	40	0	0.00	heart (T-1)	0.00
					ischemic
heart (T-14) non-	29422	37.97	0.31	0.62	heart (T-14) non-	0.62
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	37.3	0.47	0.94	heart (T-3399)	0.94
					DCM
adenoid GW99-269	26162	32.94	7.02	14.04	adenoid
tonsil GW98-280	22582	31.93	13.09	26.18	tonsil
T cells PC00314	28453	33.8	4.09	8.18	T cells
PBMNC		38.2	0.26	0.26	PBMNC
monocyte		37.25	0.48	0.96	monocyte
B cells PC00665	28455	34.27	3.06	6.12	B cells
dendritic cells	28441	36.7	0.67	1.34	dendritic cells
neutrophils	28440	33.4	5.25	5.25	neutrophils
eosinophils	28446	37.21	0.49	0.98	eosinophils
BM unstim		35.01	1.93	1.93	BM unstim
BM stim	treated	38.15	0.27	0.27	BM stim	−7.15
osteo dif	treated	40	0	0.00	osteo dif	0.00
osteo undif		40	0	0.00	osteo undif
chondrocytes		35.2	1.72	4.30	chondrocytes
OA Synovium IP12/01	29462	37.23	0.49	0.49	OA Synovium
OA Synovium NP10/01	29461	35.26	1.65	3.30	OA Synovium
OA Synovium NP57/00	28464	34.19	3.22	6.44	OA Synovium
RA Synovium	28466	36.43	0.8	1.60	RA Synovium
NP03/01
RA Synovium	28467	35.53	1.4	2.80	RA Synovium
NP71/00
RA Synovium	28475	34.14	3.32	6.64	RA Synovium
NP45/00
OA bone (biobank)	29217	36.22	0.91	0.91	OA bone
					(biobank)
OA bone Sample 1	J. Emory	34.28	3.05	6.10	OA bone
OA bone Sample 2	J. Emory	34.09	3.42	6.84	OA bone
Cartilage (pool)	Normal	35.74	1.23	2.46	Nml Cartilage
					(pool)
Cartilage (pool)	OA	34.69	2.36	4.72	OA Cartilage	1.92
					(pool)
PBL unifected	28441	35.36	1.55	3.10	PBL unifected
PBL HIV IIIB	28442	39.79	0.1	0.20	PBL HIV IIIB	−15.50
MRC5 uninfected	29158	37.66	0.37	0.74	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	30.9	24.88	49.76	MRC5 HSV	67.24
					strain F
W12 cells	29179	33.33	5.48	10.96	W12 cells	5.27
Keratinocytes	29180	36.01	1.04	2.08	Keratinocytes
B-actin control		27.27	238.18
genomic		24.3	1512.29
1.00E+05		17.99	100000
1.00E+05		17.88	100000
1.00E+04		20.91	10000
1.00E+04		20.93	10000
1.00E+03		24.55	1000
1.00E+03		24.53	1000
1.00E+02		28.88	100
1.00E+02		29.32	100
1.00E+01		32.52	10
1.00E+01		32.17	10
1.00E+00		40.	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0217] Gene Name gsk1810944BrCaAg

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      24.98
colon tumor                      2.69
colon tumor                      −1.42
colon tumor                      4.69
lung tumor                       45.91
lung tumor                       −4.88
lung tumor                       7.28
lung tumor                       −13.19
breast tumor                     −1.18
breast tumor                     8.62
breast tumor                     29.67
breast tumor                     4.45
brain stage 5 ALZ                −9.33
brain stage 5 ALZ                7.18
brain stage 5 ALZ                3.67
brain stage 5 ALZ                5.25
lung 24 COPD                     −3.56
lung 28 COPD                     −49.90
lung 23 COPD                     −1.96
asthmatic lung                   −6.02
asthmatic lung                   −1.94
asthmatic lung                   2.98
asthmatic lung                   2.57
endo VEGF                        0.26
endo bFGF                        1.48
heart (T-1) ischemic             0.00
heart (T-14) non-obstructive DCM 0.62
heart (T-3399) DCM               0.94
BM stim                          −7.15
osteo dif                        0.00
OA Cartilage (pool)              1.92
PBL HIV IIIB                     −15.50
MRC5 HSV strain F                67.24
W12 cells                        5.27

[0218] Gene Name sbg1535914AIP

[0219] Moderate to high overall expression. The highest normal expression is seen in the whole brain, fetal liver, and thymus. Expression in the disease samples is fairly ubiquitous with the highest levels seen in the uninfected PBLs, one of the normal brain samples, and one of the Alzheimer's brain samples. Upregulation in 3 of 4 asthmatic lung samples with corroborating high expression in the eosinophils and neutrophils indicates a possible involvement in asthma. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as ischemic and obstructive DCM. Upregulated in the differentiated osteoblasts. Downregulation in the HSV-infected MRC5 cells suggests that this gene may be a host factor in HSV. High expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in all of the inflammatory immune cells implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	18 S	total
sbg1535914AIP	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	30.81, 30.46	112.89	140.5	126.70	3.06	16.34	2070.18
Adipocytes Zenbio
Subcutaneous Adipose	34.7, 34.39	10.24	12.37	11.31	0.96	52.36	591.88
Zenbio
Adrenal Gland	33.84, 39.7	17.46	0.47	8.97	0.61	81.97	734.84
Clontech
Whole Brain Clontech	22.13, 21.9	23919.64	27498.63	25709.14	7.24	6.91	177549.27
Fetal Brain Clontech	34.87, 35.44	9.21	6.49	7.85	0.48	103.95	816.01
Cerebellum Clontech	32.26, 32.26	46.02	46.07	46.05	2.17	23.04	1060.94
Cervix	33.36, 32.73	23.45	34.53	28.99	2.42	20.66	598.97
Colon	30.22, 30.3	162.78	154.21	158.50	2.71	18.45	2924.26
Endometrium	32.7, 32.66	35.11	36.06	35.59	0.73	68.21	2427.35
Esophagus	32.26, 32	46.26	54.24	50.25	1.37	36.50	1833.94
Heart Clontech	35.92, 34.35	4.83	12.73	8.78	1.32	37.88	332.58
Hypothalamus	34.73, 40	10.03	0	5.02	0.32	155.28	778.73
Ileum	31.88, 31.15	58.17	91.33	74.75	2.58	19.38	1448.64
Jejunum	29.25, 28.99	295.2	347.68	321.44	6.60	7.58	2435.15
Kidney	32.24, 32.22	46.8	47.39	47.10	2.12	23.58	1110.73
Liver	33.18, 33.58	26.14	20.44	23.29	1.50	33.33	776.33
Fetal Liver Clontech	24.96, 24.96	4158.98	4176.02	4167.50	10.40	4.81	20036.06
Lung	31.98, 31.97	54.98	55.28	55.13	2.57	19.46	1072.57
Mammary Gland	28.42, 28.17	494.03	574.4	534.22	13.00	3.85	2054.67
Clontech
Myometrium	31.79, 31.76	61.72	62.76	62.24	2.34	21.37	1329.91
Omentum	33.01, 32.19	28.97	48.27	38.62	3.94	12.69	490.10
Ovary	31.49, 31.72	74.23	64.52	69.38	4.34	11.52	799.25
Pancreas	34.55, 34.36	11.23	12.65	11.94	0.81	61.80	737.95
Head of Pancreas	34.7, 35.44	10.23	6.48	8.36	1.57	31.85	266.08
Parotid Gland	30.48, 30.27	138.5	157.39	147.95	5.48	9.12	1349.86
Placenta Clontech	30.65, 30.72	124.37	119.61	121.99	5.26	9.51	1159.60
Prostate	31.23, 31.26	86.97	85.43	86.20	3.00	16.67	1436.67
Rectum	31.27, 30.53	85.15	134.2	109.68	1.23	40.65	4458.33
Salivary Gland	29.47, 29.11	258.68	321.3	289.99	7.31	6.84	1983.52
Clontech
Skeletal Muscle	38.36, 35.39	1.07	6.68	3.88	1.26	39.68	153.77
Clontech
Skin	35.03, 34.6	8.33	10.91	9.62	1.21	41.32	397.52
Small Intestine	34.63, 35.35	10.71	6.84	8.78	0.98	51.07	448.16
Clontech
Spleen	30.44, 30.13	141.71	171.93	156.82	4.92	10.16	1593.70
Stomach	32.74, 32.96	34.32	30.02	32.17	2.73	18.32	589.19
Testis Clontech	33.26, 33.73	24.93	18.6	21.77	0.57	87.87	1912.57
Thymus Clontech	25.09, 24.91	3849.53	4315.09	4082.31	9.89	5.06	20638.57
Thyroid	31.29, 31.03	83.71	98.77	91.24	2.77	18.05	1646.93
Trachea Clontech	29.42, 29.01	266.68	342.37	304.53	9.71	5.15	1568.10
Urinary Bladder	29.92, 29.78	195.24	213.49	204.37	5.47	9.14	1868.05
Uterus	31.03, 30.71	98.49	120.24	109.37	5.34	9.36	1024.02
genomic	26.39	1729.8
b-actin	27.81	720.39
1.00E+05	20.29	100000
1.00E+05	20.08	100000
1.00E+04	23.44	10000
1.00E+04	23.43	10000
1.00E+03	26.59	1000
1.00E+03	26.94	1000
1.00E+02	31.39	100
1.00E+02	30.83	100
1.00E+01	34.04	10
1.00E+01	35.19	10
1.00E+00	38.99	1
1.00E+00	40	1
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1535914AIP	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	23.65	6636.9	13273.80	colon normal
colon tumor GW98-166	21940	23.51	7202.72	14405.44	colon tumor	1.09
colon normal GW98-178	22080	26.63	1154.59	2309.18	colon normal
colon tumor GW98-177	22060	25.6	2111.16	4222.32	colon tumor	1.83
colon normal GW98-561	23514	24.39	4304.52	8609.04	colon normal
colon tumor GW98-560	23513	24.64	3700.38	7400.76	colon tumor	−1.16
colon normal GW98-894	24691	23.8	6076.66	12153.32	colon normal
colon tumor GW98-893	24690	24.02	5331.09	10662.18	colon tumor	−1.14
lung normal GW98-3	20742	24.61	3765.05	7530.10	lung normal
lung tumor GW98-2	20741	26.54	1217.31	2434.62	lung tumor	−3.09
lung normal GW97-179	20677	24.11	5059.71	10119.42	lung normal
lung tumor GW97-178	20676	24.58	3839.48	7678.96	lung tumor	−1.32
lung normal GW98-165	21922	24.23	4729.71	9459.42	lung normal
lung tumor GW98-164	21921	23.91	5676.27	11352.54	lung tumor	1.20
lung normal GW98-282	22584	24.17	4899.41	9798.82	lung normal
lung tumor GW98-281	22583	25.48	2261.81	4523.62	lung tumor	−2.17
breast normal GW00-392	28750	25.28	2554.11	2554.11	breast normal
breast tumor GW00-391	28746	25.2	2670.11	5340.22	breast tumor	2.09
breast normal GW00-413	28798	25.7	1989.13	1989.13	breast normal
breast tumor GW00-412	28797	24.78	3421.72	6843.44	breast tumor	3.44
breast normal GW00-	27592-95	27.03	909.61	909.61	breast normal
235:238
breast tumor GW00-	27588-91	25.34	2458.97	2458.97	breast tumor	2.70
231:234
breast normal GW98-621	23656	24.85	3281.35	6562.70	breast normal
breast tumor GW98-620	23655	25.2	2663.34	5326.68	breast tumor	−1.23
brain normal BB99-542	25507	24.33	4456.24	8912.48	brain normal
brain normal BB99-406	25509	22.86	10547.39	21094.78	brain normal
brain normal BB99-904	25546	24.16	4920.19	9840.38	brain normal
brain stage 5 ALZ BB99-	25502	25.54	2180.95	4361.90	brain stage 5 ALZ	−3.05
874
brain stage 5 ALZ BB99-	25503	22.63	12046.47	24092.94	brain stage 5 ALZ	1.81
887
brain stage 5 ALZ BB99-	25504	23.97	5500.12	11000.24	brain stage 5 ALZ	−1.21
862
brain stage 5 ALZ BB99-	25542	24.35	4400.1	8800.20	brain stage 5 ALZ	−1.51
927
CT lung	normal	25.14	2760.31	5520.62	CT lung Nml
lung 26	normal	30.29	134.92		lung 26 Nml
lung 27	normal	31.37	71.48	71.48	lung 27 Nml
lung 24	COPD	31.17	80.44	80.44	lung 24 COPD	−23.71
lung 28	COPD	29.86	173.39	173.39	lung 28 COPD	−11.00
lung 23	COPD	30.33	131.84	131.84	lung 23 COPD	−14.47
lung 25	normal	30.35	130.26	130.26	lung 25 Nml
asthmatic lung ODO3112	29321	25.53	2200.74	2200.74	asthmatic lung	1.15
asthmatic lung ODO3433	29323	25.45	2303.5	4607.00	asthmatic lung	2.42
asthmatic lung ODO3397	29322	24.16	4919	9838.00	asthmatic lung	5.16
asthmatic lung ODO4928	29325	24.81	3362.05	6724.10	asthmatic lung	3.53
endo cells	control	27.95	532.18	532.18	endo cells
endo VEGF		28.9	303.89	303.89	endo VEGF	−1.75
endo bFGF		28.09	489.39	489.39	endo bFGF	−1.09
heart Clontech	normal	27.46	710.71	1421.42	heart
heart (T-1) ischemic	29417	25.3	2516.82	5033.64	h art (T-1)	3.54
					ischemic
heart (T-14) non-	29422	26.44	1289.17	2578.34	heart (T-14) non-	1.81
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	25.73	1950.41	3900.82	heart (T-3399)	2.74
					DCM
adenoid GW99-269	26162	26.09	1583.81	3167.62	adenoid
tonsil GW98-280	22582	24.66	3655.92	7311.84	tonsil
T cells PC00314	28453	23.86	5864.93	11729.86	T cells
PBMNC		29.51	213.37	213.37	PBMNC
monocyte		28.58	366.84	733.68	monocyte
B cells PC00665	28455	25.43	2329.91	4659.82	B cells
dendritic cells	28441	23.67	6572.31	13144.62	dendritic cells
neutrophils	28440	22.21	15459.9	15459.90	neutrophils
eosinophils	28446	23.3	8144.32	16288.64	eosinophils
BM unstim		26.68	1121.85	1121.85	BM unstim
BM stim	treated	26.61	1164.69	1164.69	BM stim	1.04
osteo dif	treated	27.71	613.14	613.14	osteo dif	4.78
osteo undif		30.37	128.24	128.24	osteo undif
chondrocytes		24.62	3743.39	9358.48	chondrocytes
OA Synovium IP12/01	29462	25.4	2369.7	2369.70	OA Synovium
OA Synovium NP10/01	29461	26.02	1653.82	3307.64	OA Synovium
OA Synovium NP57/00	28464	24.91	3165.35	6330.70	OA Synovium
RA Synovium	28466	24.21	4763.55	9527.10	RA Synovium
NP03/01
RA Synovium	28467	24.4	4261.99	8523.98	RA Synovium
NP71/00
RA Synovium	28475	24.49	4048.08	8096.16	RA Synovium
NP45/00
OA bone (biobank)	29217	24.67	3647.85	3647.85	OA bone (biobank)
OA bone Sample 1	J. Emory	25.41	2364.62	4729.24	OA bone
OA bone Sample 2	J. Emory	24.34	4428.02	8856.04	OA bone
Cartilage (pool)	Normal	25.42	2344.99	4689.98	Nml Cartilage
					(pool)
Cartilage (pool)	OA	26.46	1276.84	2553.68	OA Cartilage (pool)	−1.84
PBL unifected	28441	22.06	16867.39	33734.78	PBL unifected
PBL HIV IIIB	28442	23.23	8471.06	16942.12	PBL HIV IIIB	−1.99
MRC5 uninfected	29158	24.83	3317.63	6635.26	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	29.28	243.95	487.90	MRC5 HSV strain	−13.60
					F
W12 cells	29179	24.53	3954.56	7909.12	W12 cells	−1.02
Keratinocytes	29180	24.5	4023.91	8047.82	Keratinocytes
B-actin control		27.54	675.35
genomic		26.17	1514.95
1.00E+05		19.51	100000
1.00E+05		19.62	100000
1.00E+04		22.92	10000
1.00E+04		22.94	10000
1.00E+03		26.46	1000
1.00E+03		26.25	1000
1.00E+02		30.5	100
1.00E+02		30.23	100
1.00E+01		34.13	10
1.00E+01		34.91	10
1.00E+00		40	0
1.00E+00		39.9	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0220] Gene Name sbg1535914AIP

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.09
colon tumor                      1.83
colon tumor                      −1.16
colon tumor                      −1.14
lung tumor                       −3.09
lung tumor                       −1.32
lung tumor                       1.20
lung tumor                       −2.17
breast tumor                     2.09
breast tumor                     3.44
breast tumor                     2.70
breast tumor                     −1.23
brain stage 5 ALZ                −3.05
brain stage 5 ALZ                1.81
brain stage 5 ALZ                −1.21
brain stage 5 ALZ                −1.51
lung 24 COPD                     −23.71
lung 28 COPD                     −11.00
lung 23 COPD                     −14.47
asthmatic lung                   1.15
asthmatic lung                   2.42
asthmatic lung                   5.16
asthmatic lung                   3.53
endo VEGF                        −1.75
endo bFGF                        −1.09
heart (T-1) ischemic             3.54
heart (T-14) non-obstructive DCM 1.81
heart (T-3399) DCM               2.74
BM stim                          1.04
osteo dif                        4.78
OA Cartilage (pool)              −1.84
PBL HIV IIIB                     −1.99
MRC5 HSV strain F                −13.60
W12 cells                        −1.02

[0221] Gene Name sbg1530894aCE

[0222] Moderate overall expression. The highest normal expression is seen in the whole brain, fetal brain, testis, liver, omentum, parotid gland, and ovary. The highest disease expression is seen in the Alzheimer's disease brain samples. Downregulation in the VEGF-treated endothelial cells suggests that this gene may be involved in angiogenesis. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as obstructive and non-obstructive DCM. Upregulated in the stimulated bone marrow. Upregulation in the HIV-infected PBLs and the W12 cells suggests that this gene may be a host factor in HIV and HPV. Moderate to high expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in the T cells and B cells implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	18 S	total
sbg1530894aCE	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	40, 37.11	0	1.86	0.93	3.06	16.34	15.20
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	29.07, 29	258.76	270.76	264.76	7.24	6.91	1828.45
Fetal Brain Clontech	34.98, 33.62	6.87	15.79	11.33	0.48	103.95	1177.75
Cerebellum Clontech	32.97, 34.18	23.6	11.22	17.41	2.17	23.04	401.15
Cervix	34.6, 36.44	8.66	2.79	5.73	2.42	20.66	118.29
Colon	34.11, 34.54	11.69	8.96	10.33	2.71	18.45	190.50
Endometrium	33.9, 40	13.33	0	6.67	0.73	68.21	454.64
Esophagus	36.21, 36.12	3.22	3.41	3.32	1.37	36.50	120.99
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	36.09, 40	3.46	0	1.73	0.32	155.28	268.63
Ileum	36.82, 35.49	2.22	5.02	3.62	2.58	19.38	70.16
Jejunum	32.47, 32.5	32	31.38	31.69	6.60	7.58	240.08
Kidney	36.29, 40	3.06	0	1.53	2.12	23.58	36.08
Liver	32.38, 32.77	33.92	26.57	30.25	1.50	33.33	1008.17
Fetal Liver Clontech	37.95, 37.39	1.11	1.56	1.34	10.40	4.81	6.42
Lung	36.12, 35.59	3.39	4.73	4.06	2.57	19.46	78.99
Mammary Gland	34.03, 33.25	12.27	19.84	16.06	13.00	3.85	61.75
Clontech
Myometrium	35.09, 34.77	6.41	7.79	7.10	2.34	21.37	151.71
Omentum	31.81, 31.91	47.94	45.24	46.59	3.94	12.69	591.24
Ovary	31.03, 31.75	77.48	49.85	63.67	4.34	11.52	733.47
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	31.42, 31.11	60.97	73.66	67.32	5.48	9.12	614.19
Placenta Clontech	35.03, 35.02	6.64	6.69	6.67	5.26	9.51	63.36
Prostate	35.1, 34.64	6.38	8.46	7.42	3.00	16.67	123.67
Rectum	35.71, 40	4.39	0.57	2.48	1.23	40.65	100.81
Salivary Gland Clontech	36.34, 38.19	2.98	0.95	1.97	7.31	6.84	13.44
Skeletal Muscle Clontech	40, 40	0	0	0.00	1.26	39.68	0.00
Skin	36.02, 34.31	3.61	10.32	6.97	1.21	41.32	287.81
Small Intestine Clontech	40, 40	0.15	0	0.08	0.98	51.07	3.83
Spleen	33.71, 34.31	14.95	10.32	12.64	4.92	10.16	128.40
Stomach	34.6, 34.41	8.68	9.75	9.22	2.73	18.32	168.77
Testis Clontech	34.24, 33.46	10.78	17.47	14.13	0.57	87.87	1241.21
Thymus Clontech	33.57, 33.09	16.35	21.95	19.15	9.89	5.06	96.81
Thyroid	38.55, 36.89	0.77	2.12	1.45	2.77	18.05	26.08
Trachea Clontech	33.11, 33.28	21.65	19.47	20.56	9.71	5.15	105.87
Urinary Bladder	35.33, 36.01	5.51	3.63	4.57	5.47	9.14	41.77
Uterus	32.12, 31.6	39.63	54.81	47.22	5.34	9.36	442.13
genomic	26.53	1234.26
b-actin	27.46	694.34
1.00E+05	18.91	100000
1.00E+05	19.67	100000
1.00E+04	23.04	10000
1.00E+04	23.14	10000
1.00E+03	26.9	1000
1.00E+03	26.66	1000
1.00E+02	30.73	100
1.00E+02	31.68	100
1.00E+01	33.59	10
1.00E+01	40	0
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1530894aCE	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	32.47	43.02	86.05	colon normal
colon tumor GW98-166	21940	35.38	10.86	21.73	colon tumor	−3.96
colon normal GW98-178	22080	35.51	10.24	20.49	colon normal
colon tumor GW98-177	22060	36.12	7.79	15.59	colon tumor	−1.31
colon normal GW98-561	23514	34.78	14.29	28.58	colon normal
colon tumor GW98-560	23513	35.57	9.97	19.94	colon tumor	−1.43
colon normal GW98-894	24691	33.65	24.27	48.54	colon normal
colon tumor GW98-893	24690	34.34	17.53	35.06	colon tumor	−1.38
lung normal GW98-3	20742	33.59	24.98	49.95	lung normal
lung tumor GW98-2	20741	35.31	11.21	22.43	lung tumor	−2.23
lung normal GW97-179	20677	32.3	46.80	93.60	lung normal
lung tumor GW97-178	20676	33.4	27.36	54.71	lung tumor	−1.71
lung normal GW98-165	21922	33.45	26.71	53.41	lung normal
lung tumor GW98-164	21921	35.08	12.45	24.91	lung tumor	−2.14
lung normal GW98-282	22584	35.51	10.24	20.49	lung normal
lung tumor GW98-281	22583	34.51	16.19	32.39	lung tumor	1.58
breast normal GW00-392	28750	32.3	46.80	46.80	breast normal
breast tumor GW00-391	28746	31.59	66.84	133.68	breast tumor	2.86
breast normal GW00-413	28798	31.6	66.50	66.50	breast normal
breast tumor GW00-412	28797	30.56	113.74	227.47	breast tumor	3.42
breast normal GW00-	27592-95	33.29	28.84	28.8	breast normal
235:238
breast tumor GW00-	27588-91	34.2	18.72	18.72	breast tumor	−1.54
231:234
breast normal GW98-621	23656	31.35	75.54	151.07	breast normal
breast tumor GW98-620	23655	32.59	40.55	81.10	breast tumor	−1.86
brain normal BB99-542	25507	28.84	287.95	575.91	brain normal
brain normal BB99-406	25509	29.79	171.25	342.49	brain normal
brain normal BB99-904	25546	29.31	222.20	444.40	brain normal
brain stage 5 ALZ BB99-	25502	30.49	118.00	235.99	brain stage 5 ALZ	−1.92
874
brain stage 5 ALZ BB99-	25503	29.61	188.72	377.45	brain stage 5 ALZ	−1.20
887
brain stage 5 ALZ BB99-	25504	30.64	109.07	218.13	brain stage 5 ALZ	−2.08
862
brain stage 5 ALZ BB99-	25542	29.24	230.89	461.77	brain stage 5 ALZ	1.02
927
CT lung	normal	35.75	9.19	18.39	CT lung Nml
lung 26	normal	32	54.36		lung 26 Nml
lung 27	normal	38.39	2.93	2.93	lung 27 Nml
lung 24	COPD	36.31	7.17	7.17	lung 24 COPD	−1.20
lung 28	COPD	37.17	4.92	4.92	lung 28 COPD	−1.74
lung 23	COPD	40	1.52	1.52	lung 23 COPD	−5.66
lung 25	normal	37.41	4.44	4.44	lung 25 Nml
asthmatic lung ODO3112	29321	35.92	8.52	8.52	asthmatic lung	−1.01
asthmatic lung ODO3433	29323	35.23	11.63	23.26	asthmatic lung	2.71
asthmatic lung ODO3397	29322	36.73	5.96	11.92	asthmatic lung	1.39
asthmatic lung ODO4928	29325	35.86	8.75	17.50	asthmatic lung	2.04
endo cells	control	36.71	6.01	6.01	endo cells
endo VEGF		40	1.52	1.52	endo VEGF	−3.96
endo bFGF		36.68	6.09	6.09	endo bFGF	1.01
heart Clontech	normal	36.29	7.23	14.46	heart
heart (T-1) ischemic	29417	35.59	9.88	19.76	heart (T-1)	1.37
					ischemic
heart (T-14) non-	29422	34.39	17.12	34.25	heart (T-14) non-	2.37
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	33.35	28.02	56.04	heart (T-3399)	3.88
					DCM
adenoid GW99-269	26162	33.95	21.05	42.10	adenoid
tonsil GW98-280	22582	32.25	47.98	95.96	tonsil
T cells PC00314	28453	32.71	38.23	76.46	T cells
PBMNC		34.51	16.19	16.19	PBMNC
monocyte		33.47	26.45	52.90	monocyte
B cells PC00665	28455	31.76	61.33	122.66	B cells
dendritic cells	28441	40	1.52	3.04	dendritic cells
neutrophils	28440	34.62	15.39	15.39	neutrophils
eosinophils	28446	35.03	12.74	25.48	eosinophils
BM unstim		40	1.52	1.52	BM unstim
BM stim	treated	35.21	11.74	11.74	BM stim	7.73
osteo dif	treated	40	1.52	1.52	osteo dif	1.00
osteo undif		40	1.52	1.52	osteo undif
chondrocytes		33.32	28.43	71.07	chondrocytes
OA Synovium IP12/01	29462	32.37	45.20	45.20	OA Synovium
OA Synovium NP10/01	29461	33.53	25.70	51.41	OA Synovium
OA Synovium NP57/00	28464	32.35	45.66	91.31	OA Synovium
RA Synovium	28466	35.01	12.86	25.72	RA Synovium
NP03/01
RA Synovium	28467	35	12.92	25.83	RA Synovium
NP71/00
RA Synovium	28475	35.89	8.64	17.27	RA Synovium
NP45/00
OA bone (biobank)	29217	36.92	5.49	5.49	OA bone
					(biobank)
OA bone Sample 1	J. Emory	34.64	15.25	30.49	OA bone
OA bone Sample 2	J. Emory	34.46	16.58	33.15	OA bone
Cartilage (pool)	Normal	33.85	22.07	44.14	Nml Cartilage
					(pool)
Cartilage (pool)	OA	37.6	4.09	8.19	OA Cartilage	−5.39
					(pool)
PBL unifected	28441	40	1.52	3.04	PBL unifected
PBL HIV IIIB	28442	36.23	7.42	14.85	PBL HIV IIIB	4.89
MRC5 uninfected	29158	40	1.52	3.04	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	40	1.52	3.04	MRC5 HSV	1.00
					strain F
W12 cells	29179	34.5	16.27	32.54	W12 cells	2.68
Keratinocytes	29180	36.69	6.06	12.13	Keratinocytes
B-actin control		27	828.79
genomic		25.75	1772.38	Copy number was calculated from the
				standard curve run on the normal plate
1.00E+05
1.00E+05
1.00E+04
1.00E+04
1.00E+03
1.00E+03
1.00E+02
1.00E+02
1.00E+01
1.00E+01
1.00E+00
1.00E+00
NTC		40	1.52
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0223] Gene Name sbg1530894aCE

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −3.96
colon tumor                      −1.31
colon tumor                      −1.43
colon tumor                      −1.38
lung tumor                       −2.23
lung tumor                       −1.71
lung tumor                       −2.14
lung tumor                       1.58
breast tumor                     2.86
breast tumor                     3.42
breast tumor                     −1.54
breast tumor                     −1.86
brain stage 5 ALZ                −1.92
brain stage 5 ALZ                −1.20
brain stage 5 ALZ                −2.08
brain stage 5 ALZ                1.02
lung 24 COPD                     −1.20
lung 28 COPD                     −1.74
lung 23 COPD                     −5.66
asthmatic lung                   −1.01
asthmatic lung                   2.71
asthmatic lung                   1.39
asthmatic lung                   2.04
endo VEGF                        −3.96
endo bFGF                        1.01
heart (T-1) ischemic             1.37
heart (T-14) non-obstructive DCM 2.37
heart (T-3399) DCM               3.88
BM stim                          7.73
osteo dif                        1.00
OA Cartilage (pool)              −5.39
PBL HIV IIIB                     4.89
MRC5 HSV strain F                1.00
W12 cells                        2.68

[0224] Gene Name sbg1530894bCE

[0225] Unlike sbg1530894aCE, Sybrman experiment failed using the primers specific for sbg1530894bCE. Taqman was performed by using the primers present in both sbg1530894aCE and sbg1530894bCE and the results is shown here as sbg1530894CE.

[0226] Moderate to low overall expression. The highest normal expression is seen in the whole brain, hypothalamus, omentum, ovary, testis and uterus. The highest disease expression is seen in the Alzheimer's disease brain samples. Upregulation in 2 of 4 lung tumor samples and 2 of 4 breast tumor samples implicates this gene in cancers of the lung and breast. Downregulation in the VEGF and bFGF-treated endothelial cells suggests that this gene may be involved in angiogenesis. Upregulation in 2 of 3 disease heart samples implies an involvement in cardiovascular diseases such as ischemic and non-obstructive DCM. Downregulated in the stimulated bone marrow and the differentiated osteoblasts. Downregulation in the HIV-infected PBLs suggests that this gene may be a host factor in HIV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	18 S	total
sbg1530894CE	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	40, 37.59	0	4.45	2.23	3.06	16.34	36.36
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland	40, 40	0	0	0.00	0.61	81.97	0.00
Clontech
Whole Brain Clontech	30.6, 30.61	341.62	340.48	341.05	7.24	6.91	2355.32
Fetal Brain Clontech	37.44, 37.27	4.89	5.41	5.15	0.48	103.95	535.34
Cerebellum Clontech	40, 37.19	0	5.71	2.86	2.17	23.04	65.78
Cervix	35.49, 34.95	16.35	22.86	19.61	2.42	20.66	405.06
Colon	38.35, 40	2.77	0	1.39	2.71	18.45	25.55
Endometrium	36.78, 36.6	7.36	8.22	7.79	0.73	68.21	531.38
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	35.17, 36.99	20.02	6.46	13.24	0.32	155.28	2055.90
Ileum	35.18, 36.3	19.85	9.89	14.87	2.58	19.38	288.18
Jejunum	34.78, 34.01	25.48	41.1	33.29	6.60	7.58	252.20
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	35.78, 36.65	13.69	7.98	10.84	1.50	33.33	361.17
Fetal Liver Clontech	40, 40	0	0	0.00	10.40	4.81	0.00
Lung	40, 40	0	0	0.00	2.57	19.46	0.00
Mammary Gland	40, 40	0	0	0.00	13.00	3.85	0.00
Clontech
Myometrium	40, 36.48	0	8.88	4.44	2.34	21.37	94.87
Omentum	32.21, 31.71	126.06	171.55	148.81	3.94	12.69	1888.39
Ovary	32.77, 32.21	88.67	125.55	107.11	4.34	11.52	1233.99
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0.79	0.40	1.57	31.85	12.58
Parotid Gland	35.78, 36.51	13.67	8.7	11.19	5.48	9.12	102.05
Placenta Clontech	40, 40	0	0	0.00	5.26	9.51	0.00
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland	40, 37.1	0	6.04	3.02	7.31	6.84	20.66
Clontech
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine	40, 40	0	0	0.00	0.98	51.07	0.00
Clontech
Spleen	36.29, 35.53	10.01	15.96	12.99	4.92	10.16	131.96
Stomach	36.68, 38.5	7.81	2.53	5.17	2.73	18.32	94.69
Testis Clontech	35.69, 35.74	14.48	14.08	14.28	0.57	87.87	1254.83
Thymus Clontech	35.24, 34.63	19.1	27.99	23.55	9.89	5.06	119.03
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	40, 40	0.35	0	0.18	9.71	5.15	0.90
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	32.08, 32.36	136.79	114.5	125.65	5.34	9.36	1176.45
genomic	28.62	1173.55
b-actin	29.41	718.23
1.00E+05	22.09	100000
1.00E+05	21.39	100000
1.00E+04	25	10000
1.00E+04	25.06	10000
1.00E+03	28.56	1000
1.00E+03	28.68	1000
1.00E+02	32.52	100
1.00E+02	32.29	100
1.00E+01	37.18	10
1.00E+01	35.98	10
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
sbg1530894CE	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	36.94	10.38	20.76	colon normal
colon tumor GW98-166	21940	40	0	0.00	colon tumor	−20.76
colon normal GW98-178	22080	40	0	0.00	colon normal
colon tumor GW98-177	22060	40	0	0.00	colon tumor	0.00
colon normal GW98-561	23514	40	0	0.00	colon normal
colon tumor GW98-560	23513	40	0	0.00	colon tumor	0.00
colon normal GW98-894	24691	40	0	0.00	colon normal
colon tumor GW98-893	24690	40	0.97	1.94	colon tumor	1.94
lung normal GW98-3	20742	36.92	10.53	21.06	lung normal
lung tumor GW98-2	20741	36.73	11.69	23.38	lung tumor	1.11
lung normal GW97-179	20677	40	0	0.00	lung normal
lung tumor GW97-178	20676	36.56	12.95	25.90	lung tumor	25.90
lung normal GW98-165	21922	40	0	0.00	lung normal
lung tumor GW98-164	21921	36.61	12.58	25.16	lung tumor	25.16
lung normal GW98-282	22584	40	0	0.00	lung normal
lung tumor GW98-281	22583	40	0	0.00	lung tumor	0.00
breast normal GW00-392	28750	40	0	0.00	breast normal
breast tumor GW00-391	28746	40	0	0.00	breast tumor	0.00
breast normal GW00-413	28798	34.58	40.93	40.93	breast normal
breast tumor GW00-412	28797	30.68	395.58	791.16	breast tumor	19.33
breast normal GW00-	27592-95	40	0	0.00	breast normal
235:238
breast tumor GW00-	27588-91	36.11	16.78	16.78	breast tumor	16.78
231:234
breast normal GW98-621	23656	40	0	0.00	breast normal
breast tumor GW98-620	23655	40	0	0.00	breast tumor	0.00
brain normal BB99-542	25507	40	1.4	2.80	brain normal
brain normal BB99-406	25509	31.15	300.7	601.40	brain normal
brain normal BB99-904	25546	30.99	330.39	660.78	brain normal
brain stage 5 ALZ BB99-	25502	32.9	108.75	217.50	brain stage 5 ALZ	−1.94
874
brain stage 5 ALZ BB99-	25503	32.2	163.47	326.94	brain stage 5 ALZ	−1.29
887
brain stage 5 ALZ BB99-	25504	32.28	156.13	312.26	brain stage 5 ALZ	−1.35
862
brain stage 5 ALZ BB99-	25542	31.19	292.69	585.38	brain stage 5 ALZ	1.39
927
CT lung	normal	40	0	0.00	CT lung Nml
lung 26	normal	40	0		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	36.44	13.88	13.88	lung 24 COPD	2.16
lung 28	COPD	40	0	0.00	lung 28 COPD	−6.41
lung 23	COPD	40	0	0.00	lung 23 COPD	−6.41
lung 25	normal	35.88	19.24	19.24	lung 25 Nml
asthmatic lung ODO3112	29321	37.87	6.05	6.05	asthmatic lung	−1.06
asthmatic lung ODO3433	29323	40	0	0.00	asthmatic lung	−6.41
asthmatic lung ODO3397	29322	37.04	9.8	19.60	asthmatic lung	3.06
asthmatic lung ODO4928	29325	36.4	14.17	28.34	asthmatic lung	4.42
endo cells	control	37.07	9.63	9.63	endo cells
endo VEGF		40	0	0.00	endo VEGF	−9.63
endo bFGF		40	0	0.00	endo bFGF	−9.63
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	36.25	15.47	30.94	heart (T-1)	30.94
					ischemic
heart (T-14) non-	29422	37.11	9.4	18.80	heart (T-14) non-	18.80
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	40	0	0.00	heart (T-3399)	0.00
					DCM
adenoid GW99-269	26162	34.58	40.89	81.78	adenoid
tonsil GW98-280	22582	40	0	0.00	tonsil
T cells PC00314	28453	40	0	0.00	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		35.66	21.89	43.78	monocyte
B cells PC00665	28455	35.23	28.01	56.02	B cells
dendritic cells	28441	35.07	30.82	61.64	dendritic cells
neutrophils	28440	40	0	0.00	neutrophils
eosinophils	28446	40	0	0.00	eosinophils
BM unstim		38.2	5	5.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	−5.00
osteo dif	treated	40	0	0.00	osteo dif	−11.25
osteo undif		36.8	11.25	11.25	osteo undif
chondrocytes		35.73	20.99	52.48	chondrocytes
OA Synovium IP12/01	29462	35.04	31.38	31.38	OA Synovium
OA Synovium NP10/01	29461	35.86	19.44	38.88	OA Synovium
OA Synovium NP57/00	28464	38.53	4.12	8.24	OA Synovium
RA Synovium	28466	40	0	0.00	RA Synovium
NP03/01
RA Synovium	28467	40	0	0.00	RA Synovium
NP71/00
RA Synovium	28475	40	0	0.00	RA Synovium
NP45/00
OA bone (biobank)	29217	35.47	24.34	24.34	OA bone (biobank)
OA bone Sample 1	J. Emory	40	0	0.00	OA bone
OA bone Sample 2	J. Emory	36.49	13.49	26.98	OA bone
Cartilage (pool)	Normal	40	0	0.00	Nml Cartilage
					(pool)
Cartilage (pool)	OA	40	0	0.00	OA Cartilage (pool)	0.00
PBL unifected	28441	35.8	20.15	40.30	PBL unifected
PBL HIV IIIB	28442	40	0	0.00	PBL HIV IIIB	−40.30
MRC5 uninfected	29158	40	0	0.00	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	40	0	0.00	MRC5 HSV strain	0.00
					F
W12 cells	29179	36.77	11.43	22.86	W12 cells	1.10
Keratinocytes	29180	36.94	10.36	20.72	Keratinocytes
B-actin control		29.12	977.73
genomic		28.27	1599.71
1.00E+05		21.56	100000
1.00E+05		21.26	100000
1.00E+04		24.94	10000
1.00E+04		25.13	10000
1.00E+03		28.75	1000
1.00E+03		28.57	1000
1.00E+02		33.51	100
1.00E+02		32.7	100
1.00E+01		40	0
1.00E+01		37.37	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0227] Gene Name sbg1530894CE

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −20.76
colon tumor                      0.00
colon tumor                      0.00
colon tumor                      1.94
lung tumor                       1.11
lung tumor                       25.90
lung tumor                       25.16
lung tumor                       0.00
breast tumor                     0.00
breast tumor                     19.33
breast tumor                     16.78
breast tumor                     0.00
brain stage 5 ALZ                −1.94
brain stage 5 ALZ                −1.29
brain stage 5 ALZ                −1.35
brain stage 5 ALZ                1.39
lung 24 COPD                     2.16
lung 28 COPD                     −6.41
lung 23 COPD                     −6.41
asthmatic lung                   −1.06
asthmatic lung                   −6.41
asthmatic lung                   3.06
asthmatic lung                   4.42
endo VEGF                        −9.63
endo bFGF                        −9.63
heart (T-1) ischemic             30.94
heart (T-14) non-obstructive DCM 18.80
heart (T-3399) DCM               0.00
BM stim                          −5.00
osteo dif                        −11.25
OA Cartilage (pool)              0.00
PBL HIV IIIB                     −40.30
MRC5 HSV strain F                0.00
W12 cells                        1.10

[0228] Gene Name gsk.1813134.NaI

[0229] Moderate to low overall expression. The highest normal expression is seen in the thyroid and trachea. The highest disease expression is seen in two of the lung tumor samples. Upregulation in 2 of 4 lung tumor samples implicates this gene in cancer of lung. Downregulation in 1 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Upregulation in 1 of 3 COPD lung samples and in 2 of 4 asthmatic lung samples implicates this gene in COPD and asthma.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
	Ct	copies	copies	Average	18 S	50 ng/	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	18 S	total
gsk.1813134.NaI	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	39:29, 40	8	0	4.00	3.06	16.34	65.36
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 39.7	2.37	6.35	4.36	0.61	81.97	357.38
Whole Brain Clontech	34.03, 34.33	154.47	130.6	142.54	7.24	6.91	984.36
Fetal Brain Clontech	37.91, 37.87	17.35	17.75	17.55	0.48	103.95	1824.32
Cerebellum Clontech	39.61, 40	6.66	3.17	4.92	2.17	23.04	113.25
Cervix	36.4, 37.11	40.8	27.25	34.03	2.42	20.66	703.00
Colon	39.3, 39.91	7.96	3.98	5.97	2.71	18.45	110.15
Endometrium	39.7, 40	6.34	1.89	4.12	0.73	68.21	280.70
Esophagus	38.1, 40	15.6	0	7.80	1.37	36.50	284.67
Heart Clontech	39.17, 40	8.55	0	4.28	1.32	37.88	161.93
Hypothalamus	40, 40	3.05	0	1.53	0.32	155.28	236.80
Ileum	40, 39.82	1.82	5.93	3.88	2.58	19.38	75.10
Jejunum	40, 37.92	1.83	17.31	9.57	6.60	7.58	72.50
Kidney	35.47, 35	68.81	89.81	79.31	2.12	23.58	1870.52
Liver	40, 40	0	2.76	1.38	1.50	33.33	46.00
Fetal Liver Clontech	40, 40	2.33	0	1.17	10.40	4.81	5.60
Lung	39.51, 38.29	7.05	14.04	10.55	2.57	19.46	205.16
Mammary Gland	39.43, 37.45	7.36	22.58	14.97	13.00	3.85	57.58
Clontech
Myometrium	39.76, 40	6.11	1.86	3.99	2.34	21.37	85.15
Omentum	39.02, 40	9.28	2.45	5.87	3.94	12.69	74.43
Ovary	37.12, 37.59	27.16	20.76	23.96	4.34	11.52	276.04
Pancreas	37.57, 39.96	21.06	2.66	11.86	0.81	61.80	733.00
Head of Pancreas	39.56, 40	6.86	1.83	4.35	1.57	31.85	138.38
Parotid Gland	39.41, 38.97	7.44	9.56	8.50	5.48	9.12	77.55
Placenta Clontech	39.21, 40	8.35	0	4.18	5.26	9.51	39.69
Prostate	39.02, 40	9.27	2.29	5.78	3.00	16.67	96.33
Rectum	39.5, 40	7.1	2.18	4.64	1.23	40.65	188.62
Salivary Gland	34.61, 33.52	111.69	206.39	159.04	7.31	6.84	1087.82
Clontech
Skeletal Muscle	40, 40	2.13	0	1.07	1.26	39.68	42.26
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine	37.24, 40	25.41	0	12.71	0.98	51.07	648.88
Clontech
Spleen	39.84, 40	5.85	2.3	4.08	4.92	10.16	41.41
Stomach	39.71, 39.83	6.32	5.9	6.11	2.73	18.32	111.90
Testis Clontech	40, 39.2	0	8.38	4.19	0.57	87.87	368.19
Thymus Clontech	35.22, 34.51	78.98	117.98	98.48	9.89	5.06	497.88
Thyroid	31.49, 31.43	648.86	672.22	660.54	2.77	18.05	11923.10
Trachea Clontech	30.33, 29.88	1245.44	1605.59	1425.52	9.71	5.15	7340.45
Urinary Bladder	38.79, 39.24	10.56	8.22	9.39	5.47	9.14	85.83
Uterus	38.49, 37.08	12.55	27.76	20.16	5.34	9.36	188.72
genomic	32.01	484.64
b-actin	27.44	6351.66
1.00E+05	21.36	100000
1.00E+05	21.76	100000
1.00E+04	25.98	10000
1.00E+04	26.16	10000
1.00E+03	31.16	1000
1.00E+03	31.66	1000
1.00E+02	37.46	100
1.00E+02	37.32	100
1.00E+01	40	0
1.00E+01	40	0
1.00E+00	39.55	1
1.00E+00	40	1
NTC	40	0
NTC	40	−1
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
gsk.1813134.NaI	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	38.23	9.78	19.56	colon normal
colon tumor GW98-166	21940	39.42	5.31	10.62	colon tumor	−1.84
colon normal GW98-178	22080	40	3.97	7.94	colon normal
colon tumor GW98-177	22060	38.84	7.14	14.27	colon tumor	1.80
colon normal GW98-561	23514	33.81	113.02	226.03	colon normal
colon tumor GW98-560	23513	37.18	17.03	34.07	colon tumor	−6.63
colon normal GW98-894	24691	35.9	34.22	68.45	colon normal
colon tumor GW98-893	24690	37.53	14.13	28.27	colon tumor	−2.42
lung normal GW98-3	20742	32.09	319.70	639.41	lung normal
lung tumor GW98-2	20741	38.85	7.10	14.20	lung tumor	−45.03
lung normal GW97-179	20677	32.76	211.84	423.69	lung normal
lung tumor GW97-178	20676	28.14	4374.08	8748.15	lung tumor	20.65
lung normal GW98-165	21922	31.8	383.06	766.13	lung normal
lung tumor GW98-164	21921	29.02	2368.86	4737.72	lung tumor	6.18
lung normal GW98-282	22584	33.45	139.87	279.74	lung normal
lung tumor GW98-281	22583	32.45	256.01	512.02	lung tumor	1.83
breast normal GW00-392	28750	35.3	47.88	47.88	breast normal
breast tumor GW00-391	28746	37.96	11.26	22.53	breast tumor	−2.13
breast normal GW00-413	28798	37.35	15.55	15.55	breast normal
breast tumor GW00-412	28797	36.67	22.43	44.85	breast tumor	2.88
breast normal GW00-	27592-95	39.85	4.28	4.28	breast normal
235:238
breast tumor GW00-	27588-91	37.01	18.66	18.66	breast tumor	4.36
231:234
breast normal GW98-621	23656	37.84	12.00	24.00	breast normal
breast tumor GW98-620	23655	36.43	25.56	51.12	breast tumor	2.13
brain normal BB99-542	25507	34.54	73.85	147.71	brain normal
brain normal BB99-406	25509	37.19	16.94	33.89	brain normal
brain normal BB99-904	25546	36.59	23.42	46.85	brain normal
brain stage 5 ALZ BB99-	25502	40	3.97	7.94	brain stage 5	−9.59
874					ALZ
brain stage 5 ALZ BB99-	25503	35.11	53.31	106.62	brain stage 5	1.40
887					ALZ
brain stage 5 ALZ BB99-	25504	36.17	29.48	58.96	brain stage 5	−1.29
862					ALZ
brain stage 5 ALZ BB99-	25542	36.63	22.92	45.84	brain stage 5	−1.66
927					ALZ
CT lung	normal	34.87	61.11	122.22	CT lung Nml
lung 26	normal	31.7	407.86		lung 26 Nml
lung 27	normal	34.62	70.53	70.53	lung 27 Nml
lung 24	COPD	34.04	98.74	98.74	lung 24 COPD	1.24
lung 28	COPD	31.97	344.47	344.47	lung 28 COPD	4.31
lung 23	COPD	34.82	62.88	62.88	lung 23 COPD	−1.27
lung 25	normal	35.34	46.81	46.81	lung 25 Nml
asthmatic lung ODO3112	29321	37.88	11.75	11.75	asthmatic lung	−6.80
asthmatic lung ODO3433	29323	34.95	58.39	116.77	asthmatic lung	1.46
asthmatic lung ODO3397	29322	33.25	157.62	315.23	asthmatic lung	3.95
asthmatic lung ODO4928	29325	31.48	468.54	937.09	asthmatic lung	11.74
endo cells	control	38.75	7.47	7.47	endo cells
endo VEGF		39.62	4.80	4.80	endo VEGF	−1.56
endo bFGF		38.7	7.67	7.67	endo bFGF	1.03
heart Clontech	normal	40	3.97	7.94	heart
heart (T-1) ischemic	29417	39.83	4.32	8.65	heart (T-1)	1.09
					ischemic
heart (T-14) non-	29422	40	3.97	7.94	heart (T-14)	1.00
obstructive DCM					non-obstructive
					DCM
heart (T-3399) DCM	29426	40	3.97	7.94	heart (T-3399)	1.00
					DCM
adenoid GW99-269	26162	37.3	15.97	31.95	adenoid
tonsil GW98-280	22582	34.42	79.15	158.31	tonsil
T cells PC00314	28453	37.26	16.32	32.64	T cells
PBMNC		40	3.97	3.97	PBMNC
monocyte		40	3.97	7.94	monocyte
B cells PC00665	28455	38.12	10.36	20.72	B cells
dendritic cells	28441	38.54	8.33	16.66	dendritic cells
neutrophils	28440	37.99	11.09	11.09	neutrophils
eosinophils	28446	38.59	8.12	16.23	eosinophils
BM unstim		39.48	5.15	5.15	BM unstim
BM stim	treated	40	3.97	3.97	BM stim	−1.30
osteo dif	treated	40	3.97	3.97	osteo dif	1.00
osteo undif		40	3.97	3.97	osteo undif
chondrocytes		39.71	4.59	11.48	chondrocytes
OA Synovium IP12/01	29462	40	3.97	3.97	OA Synovium
OA Synovium NP10/01	29461	40	3.97	7.94	OA Synovium
OA Synovium NP57/00	28464	39.68	4.66	9.32	OA Synovium
RA Synovium	28466	39.23	5.85	11.70	RA Synovium
NP03/01
RA Synovium	28467	39.08	6.31	12.63	RA Synovium
NP71/00
RA Synovium	28475	38.48	8.59	17.18	RA Synovium
NP45/00
OA bone (biobank)	29217	38.7	7.67	7.67	OA bone
					(biobank)
OA bone Sample 1	J. Emory	40	3.97	7.94	OA bone
OA bone Sample 2	J. Emory	40	3.97	7.94	OA bone
Cartilage (pool)	Normal	40	3.97	7.94	Nml Cartilage
					(pool)
Cartilage (pool)	OA	40	3.97	7.94	OA Cartilage	1.00
					(pool)
PBL unifected	28441	37.47	14.59	29.18	PBL unifected
PBL HIV IIIB	28442	37.62	13.48	26.95	PBL HIV IIIB	−1.08
MRC5 uninfected (100%)	29158	38.34	9.24	18.48	MRC5
					uninfected
					(100%)
MRC5 HSV strain F	29178	39.31	5.62	11.23	MRC5 HSV	−1.64
					strain F
W12 cells	29179	37.81	12.19	24.38	W12 cells	−1.35
Keratinocytes	29180	37.25	16.41	32.81	Keratinocytes
B-actin control		27.36	7656.40
genomic		32.01	336.00	Disease plate did not have genomic
				samples. Copy number was, therefore
				calculated from the gene-
				specific genomic curve on the normal
				plate.
1.00E+05
1.00E+05
1.00E+04
1.00E+04
1.00E+03
1.00E+03
1.00E+02
1.00E+02
1.00E+01
1.00E+01
1.00E+00
1.00E+00
NTC		39.84	4.30
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0230] Gene Name gsk.1813134.NaI

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.84
colon tumor                      1.80
colon tumor                      −6.63
colon tumor                      −2.42
lung tumor                       −45.03
lung tumor                       20.65
lung tumor                       6.18
lung tumor                       1.83
breast tumor                     −2.13
breast tumor                     2.88
breast tumor                     4.36
breast tumor                     2.13
brain stage 5 ALZ                −9.59
brain stage 5 ALZ                1.40
brain stage 5 ALZ                −1.29
brain stage 5 ALZ                −1.66
lung 24 COPD                     1.24
lung 28 COPD                     4.31
lung 23 COPD                     −1.27
asthmatic lung                   −6.80
asthmatic lung                   1.46
asthmatic lung                   3.95
asthmatic lung                   11.74
endo VEGF                        −1.56
endo bFGF                        1.03
heart (T-1) ischemic             1.09
heart (T-14) non-obstructive DCM 1.00
heart (T-3399) DCM               1.00
BM stim                          −1.30
osteo dif                        1.00
OA Cartilage (pool)              1.00
PBL HIV IIIB                     −1.08
MRC5 HSV strain F                −1.64
W12 cells                        −1.35

[0231] Gene Name gsk1811484BrCaAg

[0232] Moderate overall expression. The highest normal expression is seen in the testis, prostate, omentum, stomach, thymus, trachea, and uterus. The highest disease expression is seen in the normal and tumor breast samples. Upregulation in 2 of 4 colon tumor samples, 2 of 4 lung tumor samples, and 2 of 4 breast tumor samples implicates this gene in cancers of the colon, lung, and breast. Downregulated in the differentiated osteoblasts. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV. Patterns of expression are very similar to gsk1810944BrCaAg.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
Sample	Ct	copies	copies	Average	18 S	50 ng/	50 ng
gsk1811484-	(sample	(sample	(sample	GOI	rRNA	18 S	total
BrCaAg	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	39.41, 36.8	0.27	1.18	0.73	3.06	16.34	11.85
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0.09	0.07	0.08	0.96	52.36	4.19
Zenbio
Adrenal Gland Clontech	36.01, 40	1.84	0.06	0.95	0.61	81.97	77.87
Whole Brain Clontech	30.18, 30.41	49.07	43.24	46.16	7.24	6.91	318.75
Fetal Brain Clontech	39.33, 40	0.28	0.08	0.18	0.48	103.95	18.71
Cerebellum Clontech	37.02, 35.64	1.04	2.26	1.65	2.17	23.04	38.02
Cervix	33.68, 32.84	6.86	10.99	8.93	2.42	20.66	184.40
Colon	33.68, 33.16	6.84	9.18	8.01	2.71	18.45	147.79
Endometrium	33.7, 34.24	6.77	5	5.89	0.73	68.21	401.43
Esophagus	34.21, 33.46	5.06	7.76	6.41	1.37	36.50	233.94
Heart Clontech	37.2, 38.49	0.94	0.46	0.70	1.32	37.88	26.52
Hypothalamus	39.01, 40	0.34	0	0.17	0.32	155.28	26.40
Ileum	33.93, 34	5.96	5.7	5.83	2.58	19.38	112.98
Jejunum	30.69, 30.64	36.85	38.02	37.44	6.60	7.58	283.60
Kidney	40, 38.78	0.1	0.39	0.25	2.12	23.58	5.78
Liver	32.03, 32.1	17.32	16.62	16.97	1.50	33.33	565.67
Fetal Liver Clontech	33.75, 35.03	6.57	3.2	4.89	10.40	4.81	23.49
Lung	36.68, 37.66	1.27	0.73	1.00	2.57	19.46	19.46
Mammary Gland	31.08, 30.38	29.56	43.88	36.72	13.00	3.85	141.23
Clontech
Myometrium	34.36, 35.1	4.66	3.07	3.87	2.34	21.37	82.59
Omentum	29.38, 29.06	77.21	92.63	84.92	3.94	12.69	1077.66
Ovary	30.52, 30.53	40.65	40.27	40.46	4.34	11.52	466.13
Pancreas	37.35, 39.03	0.87	0.34	0.61	0.81	61.80	37.39
Head of Pancreas	37.45, 34.71	0.82	3.82	2.32	1.57	31.85	73.89
Parotid Gland	30.71, 31.42	36.46	24.4	30.43	5.48	9.12	277.65
Placenta Clontech	29.42, 29.08	75.34	91.24	83.29	5.26	9.51	791.73
Prostate	27.78, 27.94	189.85	173.17	181.51	3.00	16.67	3025.17
Rectum	34.4436.26,	4.46	1.6	3.03	1.23	40.65	123.17
Salivary Gland Clontech	31.26, 31.02	26.82	30.61	28.72	7.31	6.84	196.41
Skeletal Muscle	37.04, 39.4	1.03	0.27	0.65	1.26	39.68	25.79
Clontech
Skin	34.33, 36.69	4.74	1.26	3.00	1.21	41.32	123.97
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	34.01, 32.49	5.69	13.41	9.55	4.92	10.16	97.05
Stomach	30.53, 30.18	40.32	49.15	44.74	2.73	18.32	819.32
Testis Clontech	28.7, 28.22	113.42	147.88	130.65	0.57	87.87	11480.67
Thymus Clontech	28.18, 28.12	151.56	157.02	154.29	9.89	5.06	780.03
Thyroid	35.21, 35.05	2.89	3.16	3.03	2.77	18.05	54.60
Trachea Clontech	28.07, 27.71	161.13	197.69	179.41	9.71	5.15	923.84
Urinary Bladder	34.85, 33.58	3.54	7.24	5.39	5.47	9.14	49.27
Uterus	29.88, 29.45	58.18	74.16	66.17	5.34	9.36	619.57
genomic	23.84	1753.28
b-actin	27.13	273.51
1.00E+05	17.55	100000
1.00E+05	17.52	100000
1.00E+04	20.49	10000
1.00E+04	20.52	10000
1.00E+03	24.1	1000
1.00E+03	24.16	1000
1.00E+02	28.95	100
1.00E+02	28.17	100
1.00E+01	31.73	10
1.00E+01	33.09	10
1.00E+00	40	1
1.00E+00	39.14	1
NTC	38.79	−1
NTC	40	−1
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
gsk1811484-	(GSK		GOI	total		Disease
BrCaAg	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	32.98	4.15	8.30	colon normal
colon tumor GW98-166	21940	25.98	356.34	712.68	colon tumor	85.87
colon normal GW98-178	22080	34.38	1.71	3.42	colon normal
colon tumor GW98-177	22060	32.48	5.72	11.44	colon tumor	3.35
colon normal GW98-561	23514	34.77	1.34	2.68	colon normal
colon tumor GW98-560	23513	33.92	2.28	4.56	colon tumor	1.70
colon normal GW98-894	24691	32.14	7.11	14.22	colon normal
colon tumor GW98-893	24690	29.16	47.05	94.10	colon tumor	6.62
lung normal GW98-3	20742	31.62	9.86	19.72	lung normal
lung tumor GW98-2	20741	24.78	762.34	1524.68	lung tumor	77.32
lung normal GW97-179	20677	28.13	90.81	181.62	lung normal
lung tumor GW97-178	20676	31.47	10.85	21.70	lung tumor	−8.37
lung normal GW98-165	21922	30.82	16.42	32.84	lung normal
lung tumor GW98-164	21921	27.45	139.52	279.04	lung tumor	8.50
lung normal GW98-282	22584	28.47	73.15	146.30	lung normal
lung tumor GW98-281	22583	33.28	3.44	6.88	lung tumor	−21.26
breast normal GW00-392	28750	21.97	4550.43	4550.43	breast normal
breast tumor GW00-391	28746	22.72	2838.62	5677.24	breast tumor	1.25
breast normal GW00-413	28798	23.12	2188.79	2188.79	breast normal
breast tumor GW00-412	28797	21.4	6554.67	13109.34	breast tumor	5.99
breast normal GW00-	27592-95	25.84	390.48	390.48	breast normal
235:238
breast tumor GW00-	27588-91	20.99	8531.75	8531.75	breast tumor	21.85
231:234
breast normal GW98-621	23656	40	0	0.00	breast normal
breast tumor GW98-620	23655	23.15	2151.17	4302.34	breast tumor	4302.34
brain normal BB99-542	25507	32.3	6.4	12.80	brain normal
brain normal BB99-406	25509	32.95	4.23	8.46	brain normal
brain normal BB99-904	25546	33.67	2.68	5.36	brain normal
brain stage 5 ALZ BB99-	25502	33.75	2.55	5.10	brain stage 5	−1.74
874					ALZ
brain stage 5 ALZ BB99-	25503	32.35	6.22	12.44	brain stage 5	1.40
887					ALZ
brain stage 5 ALZ BB99-	25504	32.23	6.71	13.42	brain stage 5	1.51
862					ALZ
brain stage 5 ALZ BB99-	25542	32.92	4.31	8.62	brain stage 5	−1.03
927					ALZ
CT lung	normal	32.56	5.44	10.88	CT lung Nml
lung 26	normal	33.5	2.98		lung 26 Nml
lung 27	normal	37.3	0.27	0.27	lung 27 Nml
lung 24	COPD	34.74	1.36	1.36	lung 24 COPD	−2.95
lung 28	COPD	34.35	1.74	1.74	lung 28 COPD	−2.31
lung 23	COPD	32.63	5.19	5.19	lung 23 COPD	1.29
lung 25	normal	35.4	0.89	0.89	lung 25 Nml
asthmatic lung ODO3112	29321	33.73	2.58	2.58	asthmatic lung	−1.56
asthmatic lung ODO3433	29323	33.44	3.1	6.20	asthmatic lung	1.54
asthmatic lung ODO3397	29322	33.55	2.9	5.80	asthmatic lung	1.45
asthmatic lung ODO4928	29325	32.29	6.43	12.86	asthmatic lung	3.20
endo cells	control	40	0.07	0.07	endo cells
endo VEGF		40	0.11	0.11	endo VEGF	1.57
endo bFGF		40	0	0.00	endo bFGF	−0.07
heart Clontech	normal	36.11	0.57	1.14	heart
heart (T-1) ischemic	29417	37.35	0.26	0.52	heart (T-1)	−2.19
					ischemic
heart (T-14) non-	29422	38.7	0.11	0.22	heart (T-14)	−5.18
obstructive DCM					non-obstructive
					DCM
heart (T-3399) DCM	29426	35.21	1.01	2.02	heart (T-3399)	1.77
					DCM
adenoid GW99-269	26162	30.88	15.82	31.64	adenoid
tonsil GW98-280	22582	29.3	43.03	86.06	tonsil
T cells PC00314	28453	33.13	3.77	7.54	T cells
PBMNC		37.25	0.28	0.28	PBMNC
monocyte		35.73	0.72	1.44	monocyte
B cells PC00665	28455	31.41	11.3	22.60	B cells
dendritic cells	28441	35.45	0.86	1.72	dendritic cells
neutrophils	28440	32.96	4.2	4.20	neutrophils
eosinophils	28446	36.89	0.35	0.70	eosinophils
BM unstim		34.15	1.98	1.98	BM unstim
BM stim	treated	34.86	1.26	1.26	BM stim	−1.57
osteo dif	treated	40	0.14	0.14	osteo dif	−4.93
osteo undif		35.8	0.69	0.69	osteo undif
chondrocytes		32.84	4.54	11.35	chondrocytes
OA Synovium IP12/01	29462	34.19	1.93	1.93	OA Synovium
OA Synovium NP10/01	29461	34.24	1.86	3.72	OA Synovium
OA Synovium NP57/00	28464	31.49	10.71	21.42	OA Synovium
RA Synovium	28466	34.09	2.06	4.12	RA Synovium
NP03/01
RA Synovium	28467	32.6	5.28	10.56	RA Synovium
NP71/00
RA Synovium	28475	33.57	2.86	5.72	RA Synovium
NP45/00
OA bone (biobank)	29217	33.57	2.86	2.86	OA bone
					(biobank)
OA bone Sample 1	J. Emory	34.91	1.22	2.44	OA bone
OA bone Sample 2	J. Emory	32.41	5.97	11.94	OA bone
Cartilage (pool)	Normal	33.4	3.18	6.36	Nml Cartilage
					(pool)
Cartilage (pool)	OA	34.24	1.87	3.74	OA Cartilage	−1.70
					(pool)
PBL unifected	28441	36.04	0.59	1.18	PBL unifected
PBL HIV IIIB	28442	35.1	1.08	2.16	PBL HIV IIIB	1.83
MRC5 uninfected (100%)	29158	36.48	0.45	0.90	MRC5 uninfected
					(100%)
MRC5 HSV strain F	29178	29.9	29.43	58.86	MRC5 HSV	65.40
					strain F
W12 cells	29179	33.4	3.18	6.36	W12 cells	9.94
Keratinocytes	29180	37.03	0.32	0.64	Keratinocytes
B-actin control		26.65	233.22
genomic		23.36	1885.22
1.00E+05		17.13	100000
1.00E+05		17.11	100000
1.00E+04		20.04	10000
1.00E+04		22.87	10000
1.00E+03		23.43	1000
1.00E+03		23.44	1000
1.00E+02		28.05	100
1.00E+02		28.15	100
1.00E+01		31.22	10
1.00E+01		31.21	10
1.00E+00		36.14	1
1.00E+00		40	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0233] Gene Name gsk1811484BrCaAg

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      85.87
colon tumor                      3.35
colon tumor                      1.70
colon tumor                      6.62
lung tumor                       77.32
lung tumor                       −8.37
lung tumor                       8.50
lung tumor                       −21.26
breast tumor                     1.25
breast tumor                     5.99
breast tumor                     21.85
breast tumor                     4302.34
brain stage 5 ALZ                −1.74
brain stage 5 ALZ                1.40
brain stage 5 ALZ                1.51
brain stage 5 ALZ                −1.03
lung 24 COPD                     −2.95
lung 28 COPD                     −2.31
lung 23 COPD                     1.29
asthmatic lung                   −1.56
asthmatic lung                   1.54
asthmatic lung                   1.45
asthmatic lung                   3.20
endo VEGF                        1.57
endo bFGF                        −0.07
heart (T-1) ischemic             −2.19
heart (T-14) non-obstructive DCM −5.18
heart (T-3399) DCM               1.77
BM stim                          −1.57
osteo dif                        −4.93
OA Cartilage (pool)              −1.70
PBL HIV IIIB                     1.83
MRC5 HSV strain F                65.40
W12 cells                        9.94

[0234] Gene Name sbg1529984aSproteinase

[0235] Moderate to low overall expression. The highest normal expression is seen in the parotid gland. The highest disease expression is seen in one of the colon tumor samples and the neutrophils. Significant upregulation in 1 of 4 colon tumor samples is sufficient to implicate this gene in colon cancer. Downregulation in 2 of 4 lung tumor samples implicates this gene in lung cancer. Upregulation in 1 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Downregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulated in the stimulated bone marrow. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV. Patterns of expression are similar to gene sbg1529984bSproteinase

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
Sample	Ct	copies	copies	Average	18 S	50 ng/	50 ng
sbg1529984a-	(sample	(sample	(sample	GOI	rRNA	18 S	total
Sproteinase	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	37.33, 40	1.96	0	0.98	3.06	16.34	16.01
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	32.19, 31.49	54.27	85.5	69.89	7.24	6.91	482.63
Fetal Brain Clontech	40, 35.86	0	5.08	2.54	0.48	103.95	264.03
Cerebellum Clontech	36.19, 40	4.11	0	2.06	2.17	23.04	47.35
Cervix	35.93, 36.94	4.85	2.52	3.69	2.42	20.66	76.14
Colon	40, 40	0	0	0.00	2.71	18.45	0.00
Endometrium	36.09, 35.4	4.37	6.84	5.61	0.73	68.21	382.33
Esophagus	36.74, 36.68	2.88	2.99	2.94	1.37	36.50	107.12
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	36.55, 40	3.24	0	1.62	2.58	19.38	31.40
Jejunum	33.85, 34.24	18.56	14.48	16.52	6.60	7.58	125.15
Kidney	40, 40	0	0.37	0.19	2.12	23.58	4.36
Liver	36.09, 36.8	4.37	2.77	3.57	1.50	33.33	119.00
Fetal Liver Clontech	35.25, 34.9	7.5	9.42	8.46	10.40	4.81	40.67
Lung	36.23, 35.33	3.99	7.16	5.58	2.57	19.46	108.46
Mammary Gland	40, 36.9	0	2.58	1.29	13.00	3.85	4.96
Clontech
Myometrium	35.51, 40	6.34	0	3.17	2.34	21.37	67.74
Omentum	34.78, 35.94	10.16	4.81	7.49	3.94	12.69	94.99
Ovary	34.41, 35.02	12.91	8.74	10.83	4.34	11.52	124.71
Pancreas	40, 40	0.17	0.17	0.17	0.81	61.80	10.51
Head of Pancreas	36.03, 40	4.55	0	2.28	1.57	31.85	72.45
Parotid Gland	28.48, 28.05	598.21	789.07	693.64	5.48	9.12	6328.83
Placenta Clontech	38.55, 40	0.89	0.19	0.54	5.26	9.51	5.13
Prostate	40, 40	0.15	0.09	0.12	3.00	16.67	2.00
Rectum	40, 40	0	0	0.00	1.23	40.65	0.00
Salivary Gland Clontech	33.68, 33.94	20.73	17.51	19.12	7.31	6.84	130.78
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	38.8, 40	0.76	0	0.38	1.21	41.32	15.70
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	36.18, 33.96	4.13	17.37	10.75	4.92	10.16	109.25
Stomach	35.41, 40	6.79	0.46	3.63	2.73	18.32	66.39
Testis Clontech	40, 36.26	0	3.91	1.96	0.57	87.87	171.79
Thymus Clontech	34.88, 34.03	9.55	16.54	13.05	9.89	5.06	65.95
Thyroid	40, 37.19	0.09	2.14	1.12	2.77	18.05	20.13
Trachea Clontech	35.15, 34.31	8.01	13.85	10.93	9.71	5.15	56.28
Urinary Bladder	36.62, 40	3.11	0	1.56	5.47	9.14	14.21
Uterus	34.99, 33.9	8.92	17.96	13.44	5.34	9.36	125.84
genomic	27.51	1122.47
b-actin	28.3	670.96
1.00E+05	20.54	100000
1.00E+05	20.43	100000
1.00E+04	23.84	10000
1.00E+04	23.89	10000
1.00E+03	27.26	1000
1.00E+03	27.32	1000
1.00E+02	33.08	100
1.00E+02	31.79	100
1.00E+01	34.82	10
1.00E+01	35.25	10
1.00E+00	38.97	1
1.00E+00	36.41	1
NTC	40	0
NTC	36.89	−1
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1529984a-	(GSK		GOI	total		Disease
Sproteinase	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	33.01	47.63	95.26	colon normal
colon tumor GW98-166	21940	33.28	40.57	81.14	colon tumor	−1.17
colon normal GW98-178	22080	34.6	18.57	37.14	colon normal
colon tumor GW98-177	22060	23.82	11068.26	22136.52	colon tumor	596.03
colon normal GW98-561	23514	33.97	27.01	54.02	colon normal
colon tumor GW98-560	23513	33.95	27.3	54.60	colon tumor	1.01
colon normal GW98-894	24691	35.44	11.27	22.54	colon normal
colon tumor GW98-893	24690	33.01	47.81	95.62	colon tumor	4.24
lung normal GW98-3	20742	31.02	154.82	309.64	lung normal
lung tumor GW98-2	20741	39.68	0.91	1.82	lung tumor	−170.13
lung normal GW97-179	20677	32.4	68.58	137.16	lung normal
lung tumor GW97-178	20676	31.5	116.75	233.50	lung tumor	1.70
lung normal GW98-165	21922	33.82	29.54	59.08	lung normal
lung tumor GW98-164	21921	37.8	2.79	5.58	lung tumor	−10.59
lung normal GW98-282	22584	35.25	12.64	25.28	lung normal
lung tumor GW98-281	22583	35.42	11.44	22.88	lung tumor	−1.10
breast normal GW00-392	28750	33.51	35.4	35.40	breast normal
breast tumor GW00-391	28746	33.59	33.83	67.66	breast tumor	1.91
breast normal GW00-413	28798	36.84	4.93	4.93	breast normal
breast tumor GW00-412	28797	34.35	21.52	43.04	breast tumor	8.73
breast normal GW00-	27592-95	29.35	417.06	417.06	breast normal
235:238
breast tumor GW00-	27588-91	34.13	24.49	24.49	breast tumor	−17.03
231:234
breast normal GW98-621	23656	32.79	54.35	108.70	breast normal
breast tumor GW98-620	23655	35.17	13.28	26.56	breast tumor	−4.09
brain normal BB99-542	25507	33.46	36.5	73.00	brain normal
brain normal BB99-406	25509	32.74	55.8	111.60	brain normal
brain normal BB99-904	25546	34.54	19.21	38.42	brain normal
brain stage 5 ALZ BB99-	25502	32.76	55.46	110.92	brain stage 5 ALZ	1.49
874
brain stage 5 ALZ BB99-	25503	30.49	212.89	425.78	brain stage 5 ALZ	5.73
887
brain stage 5 ALZ BB99-	25504	32.66	58.74	117.48	brain stage 5 ALZ	1.58
862
brain stage 5 ALZ BB99-	25542	32.34	70.96	141.92	brain stage 5 ALZ	1.91
927
CT lung	normal	32.01	86.34	172.68	CT lung Nml
lung 26	normal	33.37	38.62		lung 26 Nml
lung 27	normal	34.67	17.88	17.88	lung 27 Nml
lung 24	COPD	36.41	6.38	6.38	lung 24 COPD	−15.13
lung 28	COPD	38.31	2.07	2.07	lung 28 COPD	−46.64
lung 23	COPD	35.21	12.97	12.97	lung 23 COPD	−7.44
lung 25	normal	31.78	99.07	99.07	lung 25 Nml
asthmatic lung ODO3112	29321	32.79	54.41	54.41	asthmatic lung	−1.77
asthmatic lung ODO3433	29323	32.38	69.2	138.40	asthmatic lung	1.43
asthmatic lung ODO3397	29322	33.12	44.79	89.58	asthmatic lung	−1.08
asthmatic lung ODO4928	29325	34.02	26.23	52.46	asthmatic lung	−1.84
endo cells	control	36.04	7.91	7.91	endo cells
endo VEGF		40	0	0.00	endo VEGF	−7.91
endo bFGF		40	0	0.00	endo bFGF	−7.91
heart Clontech	normal	33.23	41.97	83.94	heart
heart (T-1) ischemic	29417	37.79	2.8	5.60	heart (T-1)	−14.99
					ischemic
heart (T-14) non-	29422	40	0	0.00	heart (T-14) non-	−83.94
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	40	0.36	0.72	heart (T-3399)	−116.58
					DCM
adenoid GW99-269	26162	34.46	20.24	40.48	adenoid
tonsil GW98-280	22582	33.25	41.48	82.96	tonsil
T cells PC00314	28453	31.93	90.58	181.16	T cells
PBMNC		34.14	24.45	24.45	PBMNC
monocyte		32.46	66.01	132.02	monocyte
B cells PC00665	28455	32.97	48.75	97.50	B cells
dendritic cells	28441	34.32	21.93	43.86	dendritic cells
neutrophils	28440	22.25	27946.82	27946.82	neutrophils
eosinophils	28446	28.14	851.97	1703.94	eosinophils
BM unstim		33.69	31.96	31.96	BM unstim
BM stim	treated	30.89	167.46	167.46	BM stim	5.24
osteo dif	treated	40	0	0.00	osteo dif	0.00
osteo undif		40	0	0.00	osteo undif
chondrocytes		34.76	16.86	42.15	chondrocytes
OA Synovium IP12/01	29462	34.21	23.4	23.40	OA Synovium
OA Synovium NP10/01	29461	34.29	22.39	44.78	OA Synovium
OA Synovium NP57/00	28464	34.26	22.69	45.38	OA Synovium
RA Synovium	28466	37.26	3.85	7.70	RA Synovium
NP03/01
RA Synovium	28467	34.61	18.51	37.02	RA Synovium
NP71/00
RA Synovium	28475	35.47	11.11	22.22	RA Synovium
NP45/00
OA bone (biobank)	29217	29.87	307.3	307.30	OA bone (biobank)
OA bone Sample 1	J. Emory	34.01	26.31	52.62	OA bone
OA bone Sample 2	J. Emory	31.13	145.42	290.84	OA bone
Cartilage (pool)	Normal	32.76	55.43	110.86	Nml Cartilage
					(pool)
Cartilage (pool)	OA	34.04	25.93	51.86	OA Cartilage (pool)	−2.14
PBL unifected	28441	32.49	65.05	130.10	PBL unifected
PBL HIV IIIB	28442	33.92	27.87	55.74	PBL HIV IIIB	−2.33
MRC5 uninfected	29158	40	0.38	0.76	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	31.18	141.21	282.42	MRC5 HSV strain	371.61
					F
W12 cells	29179	36.9	4.77	9.54	W12 cells	16.45
Keratinocytes	29180	40	0.29	0.58	Keratinocytes
B-actin control		27.78	1057.73
genomic		27.25	1446.89
1.00E+05		20.4	100000
1.00E+05		20.39	100000
1.00E+04		23.91	10000
1.00E+04		23.82	10000
1.00E+03		27.52	1000
1.00E+03		27.36	1000
1.00E+02		32.51	100
1.00E+02		31.18	100
1.00E+01		36.29	10
1.00E+01		35.38	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0236] Gene Name sbg1529984aSproteinase

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.17
colon tumor                      596.03
colon tumor                      1.01
colon tumor                      4.24
lung tumor                       −170.13
lung tumor                       1.70
lung tumor                       −10.59
lung tumor                       −1.10
breast tumor                     1.91
breast tumor                     8.73
breast tumor                     −17.03
breast tumor                     −4.09
brain stage 5 ALZ                1.49
brain stage 5 ALZ                5.73
brain stage 5 ALZ                1.58
brain stage 5 ALZ                1.91
lung 24 COPD                     −15.13
lung 28 COPD                     −46.64
lung 23 COPD                     −7.44
asthmatic lung                   −1.77
asthmatic lung                   1.43
asthmatic lung                   −1.08
asthmatic lung                   −1.84
endo VEGF                        −7.91
endo bFGF                        −7.91
heart (T-1) ischemic             −14.99
heart (T-14) non-obstructive DCM −83.94
heart (T-3399) DCM               −116.58
BM stim                          5.24
osteo dif                        0.00
OA Cartilage (pool)              −2.14
PBL HIV IIIB                     −2.33
MRC5 HSV strain F                371.61
W12 cells                        16.45

[0237] Gene Name sbg1529984bSproteinase

[0238] Moderate to low overall expression. The highest normal expression is seen in the parotid gland. The highest disease expression is seen in one of the colon tumor samples and the neutrophils. Upregulation in 1 of 4 Alzheimer's brain samples suggests an involvement in Alzheimer's disease. Upregulation in 1 of 4 asthmatic lung samples with corroborating high expression in the neutrophils indicates a possible involvement in asthma. Downregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Downregulated in the OA cartilage pool may indicate some involvement in osteoarthritis. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV. Patterns of expression are similar to gene sbg1529984aSproteinase.

							copies of
		Mean	Mean				mRNA
		GOI	GOI				detected/
Sample	Ct	copies	copies	Average	18 S	50 ng/	50 ng
sbg1529984b-	(sample	(sample	(sample	GOI	rRNA	18 S	total
Sproteinase	1 and 2)	1)	2)	Copies	(ng)	rRNA(ng)	RNA
Subcutaneous	40, 40	0.47	0	0.24	3.06	16.34	3.84
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0.26	0.27	0.27	0.96	52.36	13.87
Zenbio
Adrenal Gland Clontech	37, 40	2.31	0	1.16	0.61	81.97	94.67
Whole Brain Clontech	32.52, 33.06	51.17	35.24	43.21	7.24	6.91	298.38
Fetal Brain Clontech	37.12, 39.18	2.12	0.51	1.32	0.48	103.95	136.69
Cerebellum Clontech	35.29, 37.27	7.52	1.92	4.72	2.17	23.04	108.76
Cervix	36.82, 36.06	2.63	4.42	3.53	2.42	20.66	72.83
Colon	38.68, 36.4	0.72	3.5	2.11	2.71	18.45	38.93
Endometrium	39.44, 36.13	0.43	4.22	2.33	0.73	68.21	158.59
Esophagus	40, 40	0	0.3	0.15	1.37	36.50	5.47
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0.28	0	0.14	0.32	155.28	21.74
Ileum	37.22, 40	1.99	0	1.00	2.58	19.38	19.28
Jejunum	34.02, 33.08	18.08	34.83	26.46	6.60	7.58	200.42
Kidney	40, 40	0	0	0.00	2.12	23.58	0.00
Liver	34.3, 35.14	14.98	8.35	11.67	1.50	33.33	388.83
Fetal Liver Clontech	35.03, 37.36	9.03	1.8	5.42	10.40	4.81	26.03
Lung	40, 40	0	0.57	0.29	2.57	19.46	5.54
Mammary Gland	38.73, 35.64	0.7	5.92	3.31	13.00	3.85	12.73
Clontech -
Myometrium	35.44, 37.27	6.79	1.92	4.36	2.34	21.37	93.06
Omentum	34.27, 35.21	15.24	7.99	11.62	3.94	12.69	147.40
Ovary	35.03, 34.35	9.05	14.47	11.76	4.34	11.52	135.48
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 36.77	0	2.72	1.36	1.57	31.85	43.31
Parotid Gland	28.68, 28.62	725.93	756.36	741.15	5.48	9.12	6762.27
Placenta Clontech	40, 40	0	0	0.00	5.26	9.51	0.00
Prostate	40, 40	0	0	0.00	3.00	16.67	0.00
Rectum	40, 40	1.34	0	0.67	1.23	40.65	27.24
Salivary Gland Clontech	34.62, 34.03	11.98	18.05	15.02	7.31	6.84	102.70
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	35.91, 35.58	4.91	6.15	5.53	4.92	10.16	56.20
Stomach	37.76, 40	1.37	0.66	1.02	2.73	18.32	18.59
Testis Clontech	35.61, 40	6.04	0	3.02	0.57	87.87	265.38
Thymus Clontech	36.63, 34.62	2.99	11.94	7.47	9.89	5.06	37.74
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	36.01, 35.55	4.59	6.32	5.46	9.71	5.15	28.09
Urinary Bladder	40, 40	0	0	0.00	5.47	9.14	0.00
Uterus	33.41, 34.08	27.65	17.46	22.56	5.34	9.36	211.19
genomic	27.92	1231.29
b-actin	28.22	998.03
1.00E+05	21.3	100000
1.00E+05	21.29	100000
1.00E+04	24.68	10000
1.00E+04	24.78	10000
1.00E+03	28.22	1000
1.00E+03	28.21	1000
1.00E+02	32.66	100
1.00E+02	32.4	100
1.00E+01	40	10
1.00E+01	34.9	10
1.00E+00	37.09	1
1.00E+00	40	0
NTC	40	−1
NTC	40	−1
				copies of
				mRNA
	Reg			detected/		Fold
Sample	number		Mean	50 ng		Change in
sbg1529984b-	(GSK		GOI	total		Disease
Sproteinase	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	33.89	30.45	60.90	colon normal
colon tumor GW98-166	21940	33.04	50.18	100.36	colon tumor	1.65
colon normal GW98-178	22080	40	0.43	0.86	colon normal
colon tumor GW98-177	22060	23.3	15255.15	30510.30	colon tumor	35477.09
colon normal GW98-561	23514	33.79	32.34	64.68	colon normal
colon tumor GW98-560	23513	35.14	14.59	29.18	colon tumor	−2.22
colon normal GW98-894	24691	35.74	10.28	20.56	colon normal
colon tumor GW98-893	24690	33.32	42.65	85.30	colon tumor	4.15
lung normal GW98-3	20742	32.85	55.9	111.80	lung normal
lung tumor GW98-2	20741	36.17	7.98	15.96	lung tumor	−7.01
lung normal GW97-179	20677	33.46	39.17	78.34	lung normal
lung tumor GW97-178	20676	33.28	43.58	87.16	lung tumor	1.11
lung normal GW98-165	21922	33.39	40.91	81.82	lung normal
lung tumor GW98-164	21921	35.46	12.1	24.20	lung tumor	−3.38
lung normal GW98-282	22584	35.86	9.56	19.12	lung normal
lung tumor GW98-281	22583	33.28	43.49	86.98	lung tumor	4.55
breast normal GW00-392	28750	33.88	30.69	30.69	breast normal
breast tumor GW00-391	28746	33.87	30.88	61.76	breast tumor	2.01
breast normal GW00-413	28798	34.84	17.43	17.43	breast normal
breast tumor GW00-412	28797	33.43	39.9	79.80	breast tumor	4.58
breast normal GW00-	27592-95	31.31	138.23	138.23	breast normal
235:238
breast tumor GW00-	27588-91	35.73	10.32	10.32	breast tumor	−13.39
231:234
breast normal GW98-621	23656	33.38	41.17	82.34	breast normal
breast tumor GW98-620	23655	37.03	4.82	9.64	breast tumor	−8.54
brain normal BB99-542	25507	33.79	32.3	64.60	brain normal
brain normal BB99-406	25509	33.02	50.66	101.32	brain normal
brain normal BB99-904	25546	36.01	8.79	17.58	brain normal
brain stage 5 ALZ BB99-	25502	34.82	17.69	35.38	brain stage 5 ALZ	−1.73
874
brain stage 5 ALZ BB99-	25503	31.51	123.39	246.78	brain stage 5 ALZ	4.03
887
brain stage 5 ALZ BB99-	25504	33.36	41.66	83.32	brain stage 5 ALZ	1.36
862
brain stage 5 ALZ BB99-	25542	34.27	24.35	48.70	brain stage 5 ALZ	−1.26
927
CT lung	normal	34.69	19.01	38.02	CT lung Nml
lung 26	normal	36.24	7.66		lung 26 Nml
lung 27	normal	34.72	18.68	18.68	lung 27 Nml
lung 24	COPD	35.99	8.88	8.88	lung 24 COPD	−2.43
lung 28	COPD	40	0	0.00	lung 28 COPD	−21.54
lung 23	COPD	36.03	8.66	8.66	lung 23 COPD	−2.49
lung 25	normal	36.19	7.92	7.92	lung 25 Nml
asthmatic lung ODO3112	29321	35.14	14.64	14.64	asthmatic lung	−1.47
asthmatic lung ODO3433	29323	31.97	93.96	187.92	asthmatic lung	8.72
asthmatic lung ODO3397	29322	35.36	12.86	25.72	asthmatic lung	1.19
asthmatic lung ODO4928	29325	34.06	27.62	55.24	asthmatic lung	2.56
endo cells	control	38.71	1.8	1.80	endo cells
endo VEGF		40	0	0.00	endo VEGF	−1.80
endo bFGF		37.98	2.77	2.77	endo bFGF	1.54
heart Clontech	normal	36.01	8.8	17.60	heart
heart (T-1) ischemic	29417	40	0	0.00	heart (T-1)	−17.60
					ischemic
heart (T-14) non-	29422	40	0.47	0.94	heart (T-14) non-	−18.72
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	40	0	0.00	heart (T-3399)	−17.60
					DCM
adenoid GW99-269	26162	36.45	6.76	13.52	adenoid
tonsil GW98-280	22582	34.4	22.64	45.28	tonsil
T cells PC00314	28453	32.3	77.45	154.90	T cells
PBMNC		34.29	24.11	24.11	PBMNC
monocyte		31.98	93.46	186.92	monocyte
B cells PC00665	28455	32.58	65.73	131.46	B cells
dendritic cells	28441	37.09	4.65	9.30	dendritic cells
neutrophils	28440	23.91	10649.45	10649.45	neutrophils
eosinophils	28446	30.49	223.9	447.80	eosinophils
BM unstim		33.78	32.43	32.43	BM unstim
BM stim	treated	32.38	73.77	73.77	BM stim	2.27
osteo dif	treated	40	0	0.00	osteo dif	0.00
osteo undif		40	0	0.00	osteo undif
chondrocytes		34.82	17.68	44.20	chondrocytes
OA Synovium IP12/01	29462	36.98	4.97	4.97	OA Synovium
OA Synovium NP10/01	29461	35.2	14.13	28.26	OA Synovium
OA Synovium NP57/00	28464	34.26	24.5	49.00	OA Synovium
RA Synovium	28466	40	0	0.00	RA Synovium
NP03/01
RA Synovium	28467	35.38	12.73	25.46	RA Synovium
NP71/00
RA Synovium	28475	36.77	5.62	11.24	RA Synovium
NP45/00
OA bone (biobank)	29217	31.73	108	108.00	OA bone
					(biobank)
OA bone Sample 1	J. Emory	35.18	14.25	28.50	OA bone
OA bone Sample 2	J. Emory	31.39	132.09	264.18	OA bone
Cartilage (pool)	Normal	34.99	15.95	31.90	Nml Cartilage
					(pool)
Cartilage (pool)	OA	40	0.46	0.92	OA Cartilage	−34.67
					(pool)
PBL unifected	28441	34.62	19.9	39.80	PBL unifected
PBL HIV IIIB	28442	34.61	19.96	39.92	PBL HIV IIIB	1.00
MRC5 uninfected	29158	36.25	7.61	15.22	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	31.57	118.66	237.32	MRC5 HSV strain	15.59
					F
W12 cells	29179	35.02	15.7	31.40	W12 cells	3.37
Keratinocytes	29180	37.09	4.66	9.32	Keratinocytes
B-actin control		27.82	1072.85
genomic		27.31	1449.65
1.00E+05		20.38	100000
1.00E+05		20.29	100000
1.00E+04		23.77	10000
1.00E+04		23.81	10000
1.00E+03		27.41	1000
1.00E+03		27.4	1000
1.00E+02		31.71	100
1.00E+02		33.6	100
1.00E+01		35.52	10
1.00E+01		35.51	10
1.00E+00		40	0
1.00E+00		40	1
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0239] Gene Name sbg1529984bSproteinase

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      1.65
colon tumor                      35477.09
colon tumor                      −2.22
colon tumor                      4.15
lung tumor                       −7.01
lung tumor                       1.11
lung tumor                       −3.38
lung tumor                       4.55
breast tumor                     2.01
breast tumor                     4.58
breast tumor                     −13.39
breast tumor                     −8.54
brain stage 5 ALZ                −1.73
brain stage 5 ALZ                4.03
brain stage 5 ALZ                1.36
brain stage 5 ALZ                −1.26
lung 24 COPD                     −2.43
lung 28 COPD                     −21.54
lung 23 COPD                     −2.49
asthmatic lung                   −1.47
asthmatic lung                   8.72
asthmatic lung                   1.19
asthmatic lung                   2.56
endo VEGF                        −1.80
endo bFGF                        1.54
heart (T-1) ischemic             −17.60
heart (T-14) non-obstructive DCM −18.72
heart (T-3399) DCM               −17.60
BM stim                          2.27
osteo dif                        0.00
OA Cartilage (pool)              −34.67
PBL HIV IIIB                     1.00
MRC5 HSV strain F                15.59
W12 cells                        3.37

[0240] Gene Name SBGKIN113 (Taqman was Performed for this Sample)

[0241] Moderate to low overall expression. The highest normal expression is seen in the omentum and in most of the samples representing the female reproductive system including the cervix, endometrium, and uterus. The highest disease expression is seen in the Alzheimer's brain samples and in the HSV-infected MRC5 cells. Significant upregulation in 1 of 4 colon tumor samples is sufficient to implicate this gene in colon cancer. Upregulation in 2 of 3 COPD lung samples and in 2 of 4 asthmatic lung samples with corroborating high expression in T cells and neutrophils implicates this gene in COPD and asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as ischemic, non-obstructive, and obstructive DCM. Downregulated in the stimulated bone marrow and the differentiated osteoblasts. Downregulation in the HIV-infected PBLs suggests that this gene may be a host factor in HIV. Upregulation in the HSV-infected MRC5 cells and the W12 cells suggests that this gene may be a host factor in HSV and HPV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50	detected/
	Ct	copies	copies	Average	18 S	ng/18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
SBGKIN113	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	37.77, 40	4.66	0	2.33	3.06	16.34	38.07
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	33.57, 33.2	60.28	75.81	68.05	7.24	6.91	469.92
Fetal Brain Clontech	40, 40	0	0	0.00	0.48	103.95	0.00
Cerebellum Clontech	40, 40	0	0	0.00	2.17	23.04	0.00
Cervix	34.88, 35.29	27.11	21.13	24.12	2.42	20.66	498.35
Colon	35.74, 37.61	16.1	5.13	10.62	2.71	18.45	195.85
Endometrium	40, 34.98	0	25.58	12.79	0.73	68.21	872.44
Esophagus	40, 36.42	0	10.58	5.29	1.37	36.50	193.07
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	37.83, 37.07	4.49	7.14	5.82	2.58	19.38	112.69
Jejunum	34.45, 33.69	35.4	56.12	45.76	6.60	7.58	346.67
Kidney	40, 37.19	0	6.62	3.31	2.12	23.58	78.07
Liver	40, 35.52	0	18.39	9.20	1.50	33.33	306.50
Fetal Liver Clontech	37.47, 37.04	5.58	7.28	6.43	10.40	4.81	30.91
Lung	37.45, 40	5.67	0	2.84	2.57	19.46	55.16
Mammary Gland	37.32, 40	6.13	0	3.07	13.00	3.85	11.79
Clontech
Myometrium	40, 37.01	0	7.37	3.69	2.34	21.37	78.74
Omentum	33.1, 31.81	80.47	176.36	128.42	3.94	12.69	1629.63
Ovary	35.21, 34.19	22.16	41.28	31.72	4.34	11.52	365.44
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	40, 40	0	0	0.00	1.57	31.85	0.00
Parotid Gland	35.26, 35.39	21.54	19.94	20.74	5.48	9.12	189.23
Placenta Clontech	36.42, 38.21	10.61	3.55	7.08	5.26	9.51	67.30
Prostate	37.83, 40	4.48	0	2.24	3.00	16.67	37.33
Rectum	40, 38.37	0	3.22	1.61	1.23	40.65	65.45
Salivary Gland Clontech	40, 40	0	0	0.00	7.31	6.84	0.00
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine Clontech	40, 40	0	0	0.00	0.98	51.07	0.00
Spleen	34.9, 34.62	26.86	31.82	29.34	4.92	10.16	298.17
Stomach	36.03, 40	13.42	0	6.71	2.73	18.32	122.89
Testis Clontech	35.78, 40	15.69	0	7.85	0.57	87.87	689.37
Thymus Clontech	33.25, 33.07	73.31	81.91	77.61	9.89	5.06	392.37
Thyroid	40, 40	0	0	0.00	2.77	18.05	0.00
Trachea Clontech	40, 40	0	0	0.00	9.71	5.15	0.00
Urinary Bladder	37.79, 40	4.58	0	2.29	5.47	9.14	20.93
Uterus	33.77, 31.89	53.34	168.09	110.72	5.34	9.36	1036.66
genomic	28.95	1012.33
b-actin	29.43	755.05
1.00E+05	21.37	100000
1.00E+05	21.69	100000
1.00E+04	25.17	10000
1.00E+04	25.26	10000
1.00E+03	28.66	1000
1.00E+03	28.69	1000
1.00E+02	32.6	100
1.00E+02	33.12	100
1.00E+01	36.63	10
1.00E+01	40	0
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
SBGKIN113	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	32.29	99.44	198.88	colon normal
colon tumor GW98-166	21940	36	11.3	22.60	colon tumor	−8.80
colon normal GW98-178	22080	33.39	51.93	103.86	colon normal
colon tumor GW98-177	22060	31.89	125.72	251.44	colon tumor	2.42
colon normal GW98-561	23514	40	0	0.00	colon normal
colon tumor GW98-560	23513	37.02	6.22	12.44	colon tumor	12.44
colon normal GW98-894	24691	35.26	17.38	34.76	colon normal
colon tumor GW98-893	24690	36.25	9.72	19.44	colon tumor	−1.79
lung normal GW98-3	20742	33.39	52.16	104.32	lung normal
lung tumor GW98-2	20741	33.74	42.52	85.04	lung tumor	−1.23
lung normal GW97-179	20677	34.21	32.18	64.36	lung normal
lung tumor GW97-178	20676	33.04	64	128.00	lung tumor	1.99
lung normal GW98-165	21922	34.22	32	64.00	lung normal
lung tumor GW98-164	21921	31.95	121.31	242.62	lung tumor	3.79
lung normal GW98-282	22584	33.02	64.86	129.72	lung normal
lung tumor GW98-281	22583	35.84	12.4	24.80	lung tumor	−5.23
breast normal GW00-392	28750	32.93	68.09	68.09	breast normal
breast tumor GW00-391	28746	34.08	34.82	69.64	breast tumor	1.02
breast normal GW00-413	28798	33.61	45.76	45.76	breast normal
breast tumor GW00-412	28797	33.13	60.66	121.32	breast tumor	2.65
breast normal GW00-	27592-95	33.26	56.26	56.26	breast normal
235:238
breast tumor GW00-	27588-91	34.16	33.24	33.24	breast tumor	−1.69
231:234
breast normal GW98-621	23656	34.18	32.83	65.66	breast normal
breast tumor GW98-620	23655	35.32	16.75	33.50	breast tumor	−1.96
brain normal BB99-542	25507	34.01	36.19	72.38	brain normal
brain normal BB99-406	25509	31.9	124.88	249.76	brain normal
brain normal BB99-904	25546	34.98	20.53	41.06	brain normal
brain stage 5 ALZ BB99-	25502	33.23	57.19	114.38	brain stage 5 ALZ	−1.06
874
brain stage 5 ALZ BB99-	25503	31.38	169.5	339.00	brain stage 5 ALZ	2.80
887
brain stage 5 ALZ BB99-	25504	31.91	124.17	248.34	brain stage 5 ALZ	2.05
862
brain stage 5 ALZ BB99-	25542	31.33	174.15	348.30	brain stage 5 ALZ	2.88
927
CT lung	normal	40	0	0.00	CT lung Nml
lung 26	normal	35.19	18.16		lung 26 Nml
lung 27	normal	40	0	0.00	lung 27 Nml
lung 24	COPD	39.56	1.4	1.40	lung 24 COPD	1.40
lung 28	COPD	33.94	37.72	37.72	lung 28 COPD	37.72
lung 23	COPD	35.4	16.05	16.05	lung 23 COPD	16.05
lung 25	normal	40	0	0.00	lung 25 Nml
asthmatic lung ODO3112	29321	40	0	0.00	asthmatic lung	0.00
asthmatic lung ODO3433	29323	40	0	0.00	asthmatic lung	0.00
asthmatic lung ODO3397	29322	33.95	37.43	74.86	asthmatic lung	74.86
asthmatic lung ODO4928	29325	36.06	10.86	21.72	asthmatic lung	21.72
endo cells	control	40	0	0.00	endo cells
endo VEGF		40	0	0.00	endo VEGF	0.00
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	40	0	0.00	heart
heart (T-1) ischemic	29417	34.3	30.58	61.16	heart (T-1)	61.16
					ischemic
heart (T-14) non-	29422	35.14	18.72	37.44	heart (T-14) non-	37.44
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	35.05	19.72	39.44	heart (T-3399)	39.44
					DCM
adenoid GW99-269	26162	34.6	25.67	51.34	adenoid
tonsil GW98-280	22582	40	0	0.00	tonsil
T cells PC00314	28453	32	117.8	235.60	T cells
PBMNC		34.93	21.13	21.13	PBMNC
monocyte		36.87	6.76	13.52	monocyte
B cells PC00665	28455	33.17	59.12	118.24	B cells
dendritic cells	28441	36.89	6.68	13.36	dendritic cells
neutrophils	28440	32.49	88.11	88.11	neutrophils
eosinophils	28446	40	0	0.00	eosinophils
BM unstim		36.4	8.92	8.92	BM unstim
BM stim	treated	40	0	0.00	BM stim	−8.92
osteo dif	treated	40	0	0.00	osteo dif	−10.93
osteo undif		36.05	10.93	10.93	osteo undif
chondrocytes		33.42	51.21	128.03	chondrocytes
OA Synovium IP12/01	29462	32.18	106.04	106.04	OA Synovium
OA Synovium NP10/01	29461	33.41	51.37	102.74	OA Synovium
OA Synovium NP57/00	28464	33.56	47.16	94.32	OA Synovium
RA Synovium	28466	36.28	9.55	19.10	RA Synovium
NP03/01
RA Synovium	28467	33.3	54.77	109.54	RA Synovium
NP71/00
RA Synovium	28475	34.05	35.3	70.60	RA Synovium
NP45/00
OA bone (biobank)	29217	35.46	15.46	15.46	OA bone
					(biobank)
OA bone Sample 1	J. Emory	35.93	11.75	23.50	OA bone
OA bone Sample 2	J. Emory	35.91	11.89	23.78	OA bone
Cartilage (pool)	Normal	33.66	44.36	88.72	Nml Cartilage
					(pool)
Cartilage (pool)	OA	37.18	5.64	11.28	OA Cartilage	−7.87
					(pool)
PBL unifected	28441	35.46	15.44	30.88	PBL unifected
PBL HIV IIIB	28442	40	0	0.00	PBL HIV IIIB	−30.88
MRC5 uninfected (100%)	29158	40	0	0.00	MRC5 uninfected
					(100%)
MRC5 HSV strain F	29178	31.45	162.22	324.44	MRC5 HSV	324.44
					strain F
W12 cells	29179	34.32	30.17	60.34	W12 cells	60.34
Keratinocytes	29180	40	0	0.00	Keratinocytes
B-actin control		28.82	758.28
genomic		28.02	1210.45
1.00E+05		20.55	100000
1.00E+05		20.69	100000
1.00E+04		24.32	10000
1.00E+04		24.25	10000
1.00E+03		28.04	1000
1.00E+03		27.94	1000
1.00E+02		33.09	100
1.00E+02		32.73	100
1.00E+01		36.83	10
1.00E+01		35.06	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0242] Gene Name SBGKIN113

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −8.80
colon tumor                      2.42
colon tumor                      12.44
colon tumor                      −1.79
lung tumor                       −1.23
lung tumor                       1.99
lung tumor                       3.79
lung tumor                       −5.23
breast tumor                     1.02
breast tumor                     2.65
breast tumor                     −1.69
breast tumor                     −1.96
brain stage 5 ALZ                −1.06
brain stage 5 ALZ                2.80
brain stage 5 ALZ                2.05
brain stage 5 ALZ                2.88
lung 24 COPD                     1.40
lung 28 COPD                     37.72
lung 23 COPD                     16.05
asthmatic lung                   0.00
asthmatic lung                   0.00
asthmatic lung                   74.86
asthmatic lung                   21.72
endo VEGF                        0.00
endo bFGF                        0.00
heart (T-1) ischemic             61.16
heart (T-14) non-obstructive DCM 37.44
heart (T-3399) DCM               39.44
BM stim                          −8.92
osteo dif                        −10.93
OA Cartilage (pool)              −7.87
PBL HIV IIIB                     −30.88
MRC5 HSV strain F                324.44
W12 cells                        60.34

[0243] Gene Name gsk305961GDNa

[0244] Moderate overall expression. The highest normal expression is seen in the whole brain, fetal liver, and thymus. This gene is expressed fairly ubiquitously in the disease samples with the highest expression seen in the normal and disease brain samples. Significant upregulation in 1 of 4 colon tumor samples is sufficient to implicate this gene in colon cancer. Upregulation in 4 of 4 asthmatic lung samples with corroborating high expression in the neutrophils indicates a possible involvement in asthma. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as ischemic, non-obstructive, and obstructive DCM. Upregulated in the stimulated bone marrow. Downregulation in the HIV-infected PBLs suggests that this gene may be a host factor in HIV. Moderate expression in the RA and OA synovium samples, the OA bone samples, and the chondrocytes with corroborating high expression in T cells, B cells, and neutrophils implicates this gene in osteoarthritis and rheumatoid arthritis.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
gsk305961GDNa	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	32.72, 32.61	51.9	55.65	53.78	3.06	16.34	878.68
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	0	0	0.00	0.96	52.36	0.00
Zenbio
Adrenal Gland	36.16, 40	5.89	0.22	3.06	0.61	81.97	250.41
Clontech
Whole Brain Clontech	26.92, 26.79	2015.63	2193.88	2104.76	7.24	6.91	14535.60
Fetal Brain Clontech	40, 36.32	0	5.32	2.66	0.48	103.95	276.51
Cerebellum Clontech	32.18, 34.16	72.63	20.87	46.75	2.17	23.04	1077.19
Cervix	33.63, 35.29	29.2	10.2	19.70	2.42	20.66	407.02
Colon	33.52, 33.7	31.18	27.97	29.58	2.71	18.45	545.66
Endometrium	40, 34.82	0	13.78	6.89	0.73	68.21	469.99
Esophagus	35.08, 36.31	11.69	5.37	8.53	1.37	36.50	311.31
Heart Clontech	34.03, 40	22.66	0	11.33	1.32	37.88	429.17
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	33.89, 34.69	24.7	14.95	19.83	2.58	19.38	384.21
Jejunum	30.83, 31.14	171.04	140.83	155.94	6.60	7.58	1181.33
Kidney	33.44, 35.58	32.85	8.48	20.67	2.12	23.58	487.38
Liver	34.7, 35.29	14.84	10.23	12.54	1.50	33.33	417.83
Fetal Liver Clontech	28.72, 28.57	648.19	714.17	681.18	10.40	4.81	3274.90
Lung	34.4, 32.94	17.88	45.19	31.54	2.57	19.46	613.52
Mammary Gland	32.19, 31.45	72.29	115.73	94.01	13.00	3.85	361.58
Clontech
Myometrium	33.49, 32.51	31.81	59.28	45.55	2.34	21.37	973.18
Omentum	33.2, 33.62	38.15	29.28	33.72	3.94	12.69	427.86
Ovary	33, 32.48	43.27	60.2	51.74	4.34	11.52	596.03
Pancreas	35.27, 35.07	10.34	11.76	11.05	0.81	61.80	682.94
Head of Pancreas	40, 40	0.29	0	0.15	1.57	31.85	4.62
Parotid Gland	33.39, 33.02	33.89	42.95	38.42	5.48	9.12	350.55
Placenta Clontech	33.89, 34.6	24.71	15.84	20.28	5.26	9.51	192.73
Prostate	36.23, 34.18	5.66	20.59	13.13	3.00	16.67	218.75
Rectum	34.28, 33.61	19.36	29.53	24.45	1.23	40.65	993.70
Salivary Gland	33.8, 34.21	26.16	20.22	23.19	7.31	6.84	158.62
Clontech
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	36.07, 40	6.23	0	3.12	1.21	41.32	128.72
Small Intestine	40, 40	0	0	0.00	0.98	51.07	0.00
Clontech
Spleen	32.2, 32.12	71.71	75.66	73.69	4.92	10.16	748.83
Stomach	33.35, 32.97	34.86	44.16	39.51	2.73	18.32	723.63
Testis Clontech	40, 40	0	0.32	0.16	0.57	87.87	14.06
Thymus Clontech	29.86, 29.38	316.1	426.56	371.33	9.89	5.06	1877.30
Thyroid	34.89, 34.13	13.15	21.19	17.17	2.77	18.05	309.93
Trachea Clontech	35.19, 35.4	10.86	9.54	10.20	9.71	5.15	52.52
Urinary Bladder	32.64, 32.29	54.45	68.08	61.27	5.47	9.14	560.01
Uterus	31.21, 31.42	134.07	117.35	125.71	5.34	9.36	1177.06
genomic	27.62	1298.05
b-actin	28.32	835.8
1.00E+05	20.94	100000
1.00E+05	21.05	100000
1.00E+04	24.22	10000
1.00E+04	24.21	10000
1.00E+03	27.57	1000
1.00E+03	27.69	1000
1.00E+02	31.86	100
1.00E+02	32.15	100
1.00E+01	40	0
1.00E+01	40	0
1.00E+00	40	0
1.00E+00	40	0
NTC	40	0
NTC	40	0
				copies of
				mRNA		Fold
	Reg			detected/		Change
	number		Mean	50 ng		in
Sample	(GSK		GOI	total		Disease
gsk305961GDNa	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	28.26	802.94	1605.88	colon normal
colon tumor GW98-166	21940	28.83	567.11	1134.22	colon tumor	−1.42
colon normal GW98-178	22080	31.06	145.98	291.96	colon normal
colon tumor GW98-177	22060	27.16	1571.35	3142.70	colon tumor	10.76
colon normal GW98-561	23514	29.52	373.73	747.46	colon normal
colon tumor GW98-560	23513	30.07	267.13	534.26	colon tumor	−1.40
colon normal GW98-894	24691	28.43	725.8	1451.60	colon normal
colon tumor GW98-893	24690	30.03	273.24	546.48	colon tumor	−2.66
lung normal GW98-3	20742	28.6	653.04	1306.08	lung normal
lung tumor GW98-2	20741	31.32	124.74	249.48	lung tumor	−5.24
lung normal GW97-179	20677	28.48	700.84	1401.68	lung normal
lung tumor GW97-178	20676	28.59	657.27	1314.54	lung tumor	−1.07
lung normal GW98-165	21922	28.28	792.39	1584.78	lung normal
lung tumor GW98-164	21921	27.54	1243.98	2487.96	lung tumor	1.57
lung normal GW98-282	22584	28.91	541.48	1082.96	lung normal
lung tumor GW98-281	22583	29.46	385.64	771.28	lung tumor	−1.40
breast normal GW00-392	28750	29.65	345.37	345.37	breast normal
breast tumor GW00-391	28746	29.18	459.56	919.12	breast tumor	2.66
breast normal GW00-413	28798	29.27	434.11	434.11	breast normal
breast tumor GW00-412	28797	29.94	288.46	576.92	breast tumor	1.33
breast normal GW00-	27592-95	30.22	242.78	242.78	breast normal
235:238
breast tumor GW00-	27588-91	30.32	228.69	228.69	breast tumor	−1.06
231:234
breast normal GW98-621	23656	27.25	1489.15	2978.30	breast normal
breast tumor GW98-620	23655	27.45	1313.03	2626.06	breast tumor	−1.13
brain normal BB99-542	25507	27.92	988.42	1976.84	brain normal
brain normal BB99-406	25509	28.21	826.32	1652.64	brain normal
brain normal BB99-904	25546	28.43	723.46	1446.92	brain normal
brain stage 5 ALZ BB99-	25502	29.67	341.17	682.34	brain stage 5 ALZ	−2.48
874
brain stage 5 ALZ BB99-	25503	27.83	1043.54	2087.08	brain stage 5 ALZ	1.23
887
brain stage 5 ALZ BB99-	25504	28.59	657.15	1314.30	brain stage 5 ALZ	−1.29
862
brain stage 5 ALZ BB99-	25542	27.57	1222.93	2445.86	brain stage 5 ALZ	1.45
927
CT lung	normal	33.3	37.35	74.70	CT lung Nml
lung 26	normal	32.08	78.26		lung 26 Nml
lung 27	normal	32.45	62.64	62.64	lung 27 Nml
lung 24	COPD	33.08	42.63	42.63	lung 24 COPD	−1.87
lung 28	COPD	31.63	102.87	102.87	lung 28 COPD	1.29
lung 23	COPD	32.02	81.24	81.24	lung 23 COPD	1.02
lung 25	normal	31.64	102.34	102.34	lung 25 Nml
asthmatic lung ODO3112	29321	30.37	222.52	222.52	asthmatic lung	2.79
asthmatic lung ODO3433	29323	29.48	381.78	763.56	asthmatic lung	9.56
asthmatic lung ODO3397	29322	27.42	1336.97	2673.94	asthmatic lung	33.47
asthmatic lung ODO4928	29325	28.55	674.55	1349.10	asthmatic lung	16.89
endo cells	control	32.1	77.45	77.45	endo cells
endo VEGF		31.93	86.01	86.01	endo VEGF	1.11
endo bFGF		31.63	102.85	102.85	endo bFGF	1.33
heart Clontech	normal	35.45	10.07	20.14	heart
heart (T-1) ischemic	29417	28.37	750.43	1500.86	heart (T-1)	74.52
					ischemic
heart (T-14) non-	29422	29.5	376.98	753.96	heart (T-14) non-	37.44
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	28.63	641.88	1283.76	heart (T-3399)	63.74
					DCM
adenoid GW99-269	26162	29.72	330.41	660.82	adenoid
tonsil GW98-280	22582	28.03	922.22	1844.44	tonsil
T cells PC00314	28453	29.66	342.13	684.26	T cells
PBMNC		33.44	34.3	34.30	PBMNC
monocyte		34.41	18.95	37.90	monocyte
B cells PC00665	28455	28.69	617.46	1234.92	B cells
dendritic cells	28441	30.55	198.74	397.48	dendritic cells
neutrophils	28440	28.89	547.36	547.36	neutrophils
eosinophils	28446	32.86	48.74	97.48	eosinophils
BM unstim		34.4	19.08	19.08	BM unstim
BM stim	treated	31.71	98.33	98.33	BM stim	5.15
osteo dif	treated	32.17	74.22	74.22	osteo dif	1.75
osteo undif		33.09	42.38	42.38	osteo undif
chondrocytes		29.45	388.3	970.75	chondrocytes
OA Synovium IP12/01	29462	28.49	696.93	696.93	OA Synovium
OA Synovium NP10/01	29461	30.09	263.3	526.60	OA Synovium
OA Synovium NP57/00	28464	29.15	466.7	933.40	OA Synovium
RA Synovium	28466	29.06	493.54	987.08	RA Synovium
NP03/01
RA Synovium	28467	28.7	615.4	1230.80	RA Synovium
NP71/00
RA Synovium	28475	29.13	473.65	947.30	RA Synovium
NP45/00
OA bone (biobank)	29217	30	278.56	278.56	OA bone (biobank)
OA bone Sample 1	J. Emory	28.74	600.15	1200.30	OA bone
OA bone Sample 2	J. Emory	29.24	442.37	884.74	OA bone
Cartilage (pool)	Normal	28.1	887.94	1775.88	Nml Cartilage
					(pool)
Cartilage (pool)	OA	30.04	271.28	542.56	OA Cartilage (pool)	−3.27
PBL unifected	28441	27.65	1165.13	2330.26	PBL unifected
PBL HIV IIIB	28442	29.8	313.52	627.04	PBL HIV IIIB	−3.72
MRC5 uninfected	29158	29	510.52	1021.04	MRC5 uninfected
(100%)					(100%)
MRC5 HSV strain F	29178	29.46	387.61	775.22	MRC5 HSV strain F	−1.32
W12 cells	29179	27.76	1090.41	2180.82	W12 cells	1.28
Keratinocytes	29180	28.16	853.46	1706.92	Keratinocytes
B-actin control		28.21	830.7
genomic		27.27	1465.59
1.00E+05		20.48	100000
1.00E+05		20.43	100000
1.00E+04		23.9	10000
1.00E+04		24.02	10000
1.00E+03		27.55	1000
1.00E+03		27.46	1000
1.00E+02		32.83	100
1.00E+02		32.19	100
1.00E+01		34.68	10
1.00E+01		40	0
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0245] Gene Name gsk305961GDNa

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.42
colon tumor                      10.76
colon tumor                      −1.40
colon tumor                      −2.66
lung tumor                       −5.24
lung tumor                       −1.07
lung tumor                       1.57
lung tumor                       −1.40
breast tumor                     2.66
breast tumor                     1.33
breast tumor                     −1.06
breast tumor                     −1.13
brain stage 5 ALZ                −2.48
brain stage 5 ALZ                1.23
brain stage 5 ALZ                −1.29
brain stage 5 ALZ                1.45
lung 24 COPD                     −1.87
lung 28 COPD                     1.29
lung 23 COPD                     1.02
asthmatic lung                   2.79
asthmatic lung                   9.56
asthmatic lung                   33.47
asthmatic lung                   16.89
endo VEGF                        1.11
endo bFGF                        1.33
heart (T-1) ischemic             74.52
heart (T-14) non-obstructive DCM 37.44
heart (T-3399) DCM               63.74
BM stim                          5.15
osteo dif                        1.75
OA Cartilage (pool)              −3.27
PBL HIV IIIB                     −3.72
MRC5 HSV strain F                −1.32
W12 cells                        1.28

[0246] Gene Name gsk2402719—2395124BIG2

[0247] Moderate overall expression. The highest normal expression is seen in the whole brain, endometrium, fetal liver, rectum, and thymus. The highest disease expression is seen in the normal and disease brain samples. Downregulation in 1 of 4 colon tumor samples and 1 of 4 lung tumor samples implicates this gene in cancers of the colon and lung. Upregulation in 3 of 3 disease heart samples implies an involvement in cardiovascular diseases such as non-obstructive and obstructive DCM and ischemia. Upregulation in the H-infected PBLs suggests that this gene may be a host factor in HIV.

							copies of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
gsk2402719_2395124BIG2	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	35.28, 34.85	6.1	8.03	7.07	3.06	16.34	115.44
Adipocytes Zenbio
Subcutaneous Adipose	40, 40	1.25	0.96	1.11	0.96	52.36	57.85
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	23.66, 23.25	10169.71	13215.56	11692.64	7.24	6.91	80750.24
Fetal Brain Clontech	36.72, 40	2.43	1	1.72	0.48	103.95	178.27
Cerebellum Clontech	34.22, 33.46	11.99	19.44	15.72	2.17	23.04	362.10
Cervix	33.97, 32.73	14.1	31.04	22.57	2.42	20.66	466.32
Colon	33.7, 33.26	16.72	22.21	19.47	2.71	18.45	359.13
Endometrium	34.88, 33.41	7.88	20.12	14.00	0.73	68.21	954.98
Esophagus	40, 40	0	0	0.00	1.37	36.50	0.00
Heart Clontech	40, 40	0	0	0.00	1.32	37.88	0.00
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	36.05, 36.11	3.72	3.59	3.66	2.58	19.38	70.83
Jejunum	33.01, 33.17	25.92	23.51	24.72	6.60	7.58	187.23
Kidney	36.85, 35.29	2.24	6.06	4.15	2.12	23.58	97.88
Liver	34.92, 35.91	7.68	4.07	5.88	1.50	33.33	195.83
Fetal Liver Clontech	29.86, 29.34	194.37	270.22	232.30	10.40	4.81	1116.80
Lung	40, 40	0	0	0.00	2.57	19.46	0.00
Mammary Gland	31.65, 30.95	61.84	96.67	79.26	13.00	3.85	304.83
Clontech
Myometrium	34.98, 34.06	7.38	13.25	10.32	2.34	21.37	220.41
Omentum	40, 34.96	0	7.45	3.73	3.94	12.69	47.27
Ovary	33.61, 35.48	17.76	5.36	11.56	4.34	11.52	133.18
Pancreas	40, 40	0	0	0.00	0.81	61.80	0.00
Head of Pancreas	35.75, 40	4.53	0.96	2.75	1.57	31.85	87.42
Parotid Gland	34.71, 36.01	8.77	3.84	6.31	5.48	9.12	57.53
Placenta Clontech	33.98, 33.9	13.94	14.7	14.32	5.26	9.51	136.12
Prostate	40, 40	1.26	0	0.63	3.00	16.67	10.50
Rectum	34.54, 33.29	9.8	21.73	15.77	1.23	40.65	640.85
Salivary Gland Clontech	35.9, 36	4.1	3.85	3.98	7.31	6.84	27.19
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	1.42	1.24	1.33	1.21	41.32	54.96
Small Intestine Clontech	39.43, 35.47	0.43	5.4	2.92	0.98	51.07	148.88
Spleen	35.92, 40	4.06	0	2.03	4.92	10.16	20.63
Stomach	40, 33.97	0	14.08	7.04	2.73	18.32	128.94
Testis Clontech	35.01, 40	7.26	0	3.63	0.57	87.87	318.98
Thymus Clontech	29, 28.19	335.07	563.44	449.26	9.89	5.06	2271.26
Thyroid	34.82, 33.73	8.2	16.41	12.31	2.77	18.05	222.11
Trachea Clontech	33.57, 33.17	18.13	23.47	20.80	9.71	5.15	107.11
Urinary Bladder	32.07, 32.42	47.27	37.8	42.54	5.47	9.14	388.80
Uterus	32.44, 31.61	37.44	63.44	50.44	5.34	9.36	472.28
genomic	26.63	1526.9
b-actin	28.11	594.87
1.00E+05	19.85	100000
1.00E+05	19.99	100000
1.00E+04	23.47	10000
1.00E+04	23.62	10000
1.00E+03	27.23	1000
1.00E+03	27.29	1000
1.00E+02	32.1	100
1.00E+02	31.16	100
1.00E+01	40	10
1.00E+01	40	10
1.00E+00	40	0
1.00E+00	37.31	1
NTC	40	−1
NTC	40	0
				copies of
				mRNA
	Reg			detected/		Fold
	number		Mean	50 ng		Change in
Sample	(GSK		GOI	total		Disease
gsk2402719_2395124BIG2	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	26.46	2706.74	5413.48	colon normal
colon tumor GW98-166	21940	26.85	2226.83	4453.66	colon tumor	−1.22
colon normal GW98-178	22080	31	271.46	542.92	colon normal
colon tumor GW98-177	22060	30.76	306.61	613.22	colon tumor	1.13
colon normal GW98-561	23514	28	1240.55	2481.10	colon normal
colon tumor GW98-560	23513	29	749.77	1499.54	colon tumor	−1.65
colon normal GW98-894	24691	26.66	2452.21	4904.42	colon normal
colon tumor GW98-893	24690	31.94	169.13	338.26	colon tumor	−14.50
lung normal GW98-3	20742	28.13	1163	2326.00	lung normal
lung tumor GW98-2	20741	36.81	14.4	28.80	lung tumor	−80.76
lung normal GW97-179	20677	28.45	991.35	1982.70	lung normal
lung tumor GW97-178	20676	27.75	1410.69	2821.38	lung tumor	1 .42
lung normal GW98-165	21922	27.45	1643.91	3287.82	lung normal
lung tumor GW98-164	21921	29.31	638.73	1277.46	lung tumor	−2.57
lung normal GW98-282	22584	30.26	396.16	792.32	lung normal
lung tumor GW98-281	22583	29.08	719.66	1439.32	lung tumor	1.82
breast normal GW00-392	28750	29.06	727.15	727.15	breast normal
breast tumor GW00-391	28746	29.29	645.27	1290.54	breast tumor	1.77
breast normal GW00-413	28798	30.26	395.16	395.16	breast normal
breast tumor GW00-412	28797	28.42	1002.1	2004.20	breast tumor	5.07
breast normal GW00-	27592-95	30.32	383.84	383.84	breast normal
235:238
breast tumor GW00-	27588-91	28.51	961.05	961.05	breast tumor	2.50
231:234
breast normal GW98-621	23656	29.01	743.09	1486.18	breast normal
breast tumor GW98-620	23655	30.46	357.2	714.40	breast tumor	−2.08
brain normal BB99-542	25507	25.12	5327.67	10655.34	brain normal
brain normal BB99-406	25509	27.83	1351.64	2703.28	brain normal
brain normal BB99-904	25546	25.75	3870.14	7740.28	brain normal
brain stage 5 ALZ BB99-	25502	29.43	601.57	1203.14	brain stage 5 ALZ	−5.85
874
brain stage 5 ALZ BB99-	25503	26.29	2947.47	5894.94	brain stage 5 ALZ	−1.19
887
brain stage 5 ALZ BB99-	25504	26.45	2727.55	5455.10	brain stage 5 ALZ	−1.29
862
brain stage 5 ALZ BB99-	25542	26.39	2803.89	5607.78	brain stage 5 ALZ	−1.25
927
CT lung	normal	29.27	654.09	1308.18	CT lung Nml
lung 26	normal	38.1	7.47		lung 26 Nml
lung 27	normal	35.28	31.21	31.21	lung 27 Nml
lung 24	COPD	34.34	50.08	50.08	lung 24 COPD	−9.42
lung 28	COPD	35.23	31.94	31.94	lung 28 COPD	−14.77
lung 23	COPD	35.24	31.78	31.78	lung 23 COPD	−14.85
lung 25	normal	33.51	76.24	76.24	lung 25 Nml
asthmatic lung ODO3112	29321	30.3	388.5	388.50	asthmatic lung	−1.21
asthmatic lung ODO3433	29323	29.02	742.53	1485.06	asthmatic lung	3.15
asthmatic lung ODO3397	29322	27.36	1717.68	3435.36	asthmatic lung	7.28
asthmatic lung ODO4928	29325	28.12	1167.33	2334.66	asthmatic lung	4.95
endo cells	control	40	0	0.00	endo cells
endo VEGF		40	0	0.00	endo VEGF	0.00
endo bFGF		40	0	0.00	endo bFGF	0.00
heart Clontech	normal	32.49	128.22	256.44	heart
heart (T-1) ischemic	29417	28.23	1104.84	2209.68	heart (T-1)	8.62
					ischemic
heart (T-14) non-	29422	29.35	626.6	1253.20	heart (T-14) non-	4.89
obstructive DCM					obstructive DCM
heart (T-3399) DCM	29426	28.69	876.16	1752.32	heart (T-3399)	6.83
					DCM
adenoid GW99-269	26162	29.3	641.83	1283.66	adenoid
tonsil GW98-280	22582	28.29	1074.11	2148.22	tonsil
T cells PC00314	28453	35.2	32.49	64.98	T cells
PBMNC		40	0	0.00	PBMNC
monocyte		40	0	0.00	monocyte
B cells PC00665	28455	40	2.73	5.46	B cells
dendritic cells	28441	35.31	30.64	61.28	dendritic cells
neutrophils	28440	32.41	133.12	133.12	neutrophils
eosinophils	28446	37.05	12.74	25.48	eosinophils
BM unstim		40	0	0.00	BM unstim
BM stim	treated	40	0	0.00	BM stim	0.00
osteo dif	treated	40	0	0.00	osteo dif	0.00
osteo undif		40	0	0.00	osteo undif
chondrocytes		40	0	0.00	chondrocytes
OA Synovium IP12/01	29462	26.63	2484.1	2484.10	OA Synovium
OA Synovium NP10/01	29461	29.13	702.54	1405.08	OA Synovium
OA Synovium NP57/00	28464	26.22	3050.86	6101.72	OA Synovium
RA Synovium	28466	31.63	197.53	395.06	RA Synovium
NP03/01
RA Synovium	28467	29.23	665.35	1330.70	RA Synovium
NP71/00
RA Synovium	28475	31.98	165.38	330.76	RA Synovium
NP45/00
OA bone (biobank)	29217	35.46	28.46	28.46	OA bone
					(biobank)
OA bone Sample 1	J. Emory	31.87	175.21	350.42	OA bone
OA bone Sample 2	J. Emory	32.2	148.1	296.20	OA bone
Cartilage (pool)	Normal	28.88	796.08	1592.16	Nml Cartilage
					(pool)
Cartilage (pool)	OA	30.26	396.09	792.18	OA Cartilage	−2.01
					(pool)
PBL unifected	28441	40	0	0.00	PBL unifected
PBL HIV IIIB	28442	39.92	3.8	7.60	PBL HIV IIIB	7.60
MRC5 uninfected (100%)	29158	34.46	47.26	94.52	MRC5 uninfected
					(100%)
MRC5 HSV strain F	29178	34.7	41.87	83.74	MRC5 HSV	−1.13
					strain F
W12 cells	29179	40	3.66	7.32	W12 cells	1.02
Keratinocytes	29180	40	3.58	7.16	Keratinocytes
B-actin control		27.25	1815.19
genomic		27.3	1773.27
1.00E+05		20	100000
1.00E+05		20.27	100000
1.00E+04		23.63	10000
1.00E+04		23.66	10000
1.00E+03		27.18	1000
1.00E+03		27.37	1000
1.00E+02		33.46	100
1.00E+02		32.03	100
1.00E+01		39.15	10
1.00E+01		40	10
1.00E+00		40	0
1.00E+00		40	0
NTC		40	0
*lung 26 Normal has been omitted due to multiple amplification failures from that sample

[0248] Gene Name gsk2402719—2395124BIG2

                                 Fold Change in Disease
                                 Population Relative to
Disease tissues                  Normal
colon tumor                      −1.22
colon tumor                      1.13
colon tumor                      −1.65
colon tumor                      −14.50
lung tumor                       −80.76
lung tumor                       1.42
lung tumor                       −2.57
lung tumor                       1.82
breast tumor                     1.77
breast tumor                     5.07
breast tumor                     2.50
breast tumor                     −2.08
brain stage 5 ALZ                −5.85
brain stage 5 ALZ                −1.19
brain stage 5 ALZ                −1.29
brain stage 5 ALZ                −1.25
lung 24 COPD                     −9.42
lung 28 COPD                     −14.77
lung 23 COPD                     −14.85
asthmatic lung                   −1.21
asthmatic lung                   3.15
asthmatic lung                   7.28
asthmatic lung                   4.95
endo VEGF                        0.00
endo bFGF                        0.00
heart (T-1) ischemic             8.62
heart (T-14) non-obstructive DCM 4.89
heart (T-3399) DCM               6.83
BM stim                          0.00
osteo dif                        0.00
OA Cartilage (pool)              −2.01
PBL HIV IIIB                     7.60
MRC5 HSV strain F                −1.13
W12 cells                        1.02

[0249] Gene Name sbg102200MCTc

[0250] High to moderate overall expression. Highest normal expression in the whole brain, liver, fetal liver, and thymus. Highest disease expression in one of the colon normal/tumor pairs, one of the lung normal/tumor pairs, one of the asthmatic lung samples, the dendritic cells, and the uninfected and HIV-infected PBL cells. Upregulation in 2 of 4 breast tumor samples is sufficient to make a disease claim in cancer of the breast. Upregulation in 1 of 4 AD brain samples indicates a potential role in Alzheimer's disease. Downregulation in 3 of 3 COPD lung samples suggests involvement in chronic obstructive pulmonary disease. Upregulation in 1 of 4 asthmatic lung samples indicates a potential role for this gene in lung cancer. High expression in all of the immune cells. Also high to moderate expression in the OA and RA synovium samples, the OA bone samples, and in the chondrocytes suggests an involvement in osteoarthritis and rheumatoid arthritis.

							copies
							of
		Mean	Mean				mRNA
		GOI	GOI			50 ng/	detected/
	Ct	copies	copies	Average	18 S	18 S	50 ng
Sample	(sample	(sample	(sample	GOI	rRNA	rRNA	total
sbg102200MCTc	1 and 2)	1)	2)	Copies	(ng)	(ng)	RNA
Subcutaneous	35.66, 34.05	3.76	10.2	6.98	3.06	16.34	114.05
Adipocytes Zenbio
Subcutaneous Adipose	40, 36	0.17	3.07	1.62	0.96	52.36	84.82
Zenbio
Adrenal Gland Clontech	40, 40	0	0	0.00	0.61	81.97	0.00
Whole Brain Clontech	26.32, 26.41	1192.66	1124.69	1158.68	7.24	6.91	8001.90
Fetal Brain Clontech	40, 35.84	0	3.38	1.69	0.48	103.95	175.68
Cerebellum Clontech	34.51, 34.28	7.68	8.8	8.24	2.17	23.04	189.86
Cervix	40, 34.34	3.17	8.5	5.84	2.42	20.66	120.56
Colon	33.67, 35.6	12.86	3.91	8.39	2.71	18.45	154.70
Endometrium	35.32, 34.43	4.66	8.05	6.36	0.73	68.21	433.49
Esophagus	34.27, 35.14	8.86	5.19	7.03	1.37	36.50	256.39
Heart Clontech	40, 35.05	0	5.5	2.75	1.32	37.88	104.17
Hypothalamus	40, 40	0	0	0.00	0.32	155.28	0.00
Ileum	35.29, 33.68	4.74	12.8	8.77	2.58	19.38	169.96
Jejunum	31.23, 30.98	57.65	67.22	62.44	6.60	7.58	472.99
Kidney	34.67, 34.21	6.95	9.2	8.08	2.12	23.58	190.45
Liver	30.76, 30.65	77.12	82.56	79.84	1.50	33.33	2661.33
Fetal Liver Clontech	26.8, 27.1	885.14	734.31	809.73	10.40	4.81	3892.91
Lung	40, 40	0	0.17	0.09	2.57	19.46	1.65
Mammary Gland	31.28, 31.37	56.1	52.95	54.53	13.00	3.85	209.71
Clontech
Myometrium	34.16, 36.28	9.48	2.57	6.03	2.34	21.37	128.74
Omentum	34.18, 33.42	9.38	15	12.19	3.94	12.69	154.70
Ovary	34.21, 34.18	9.24	9.39	9.32	4.34	11.52	107.32
Pancreas	40, 40	0	0.14	0.07	0.81	61.80	4.33
Head of Pancreas	40, 35.02	0	5.59	2.80	1.57	31.85	89.01
Parotid Gland	31.23, 31.9	57.68	38.33	48.01	5.48	9.12	438.00
Placenta Clontech	31.77, 33.13	41.33	17.94	29.64	5.26	9.51	281.70
Prostate	39.72, 35.03	0.31	5.56	2.94	3.00	16.67	48.92
Rectum	35.36, 34.34	4.53	8.5	6.52	1.23	40.65	264.84
Salivary Gland	30.52, 30.54	89.5	88.43	88.97	7.31	6.84	608.52
Clontech
Skeletal Muscle	40, 40	0	0	0.00	1.26	39.68	0.00
Clontech
Skin	40, 40	0	0	0.00	1.21	41.32	0.00
Small Intestine	40, 39.27	0	0.41	0.21	0.98	51.07	10.47
Clontech
Spleen	34.21, 33.54	9.2	13.91	11.56	4.92	10.16	117.43
Stomach	35.05, 33.62	5.51	13.22	9.37	2.73	18.32	171.52
Testis Clontech	40, 40	0	0	0.00	0.57	87.87	0.00
Thymus Clontech	28.56, 28.44	299.45	322.02	310.74	9.89	5.06	1570.96
Thyroid	31.65, 32.3	44.76	29.81	37.29	2.77	18.05	673.01
Trachea Clontech	32.3, 31.9	29.9	38.28	34.09	9.71	5.15	175.54
Urinary Bladder	34.34, 35.02	8.49	5.59	7.04	5.47	9.14	64.35
Uterus	33.07, 34.56	18.62	7.45	13.04	5.34	9.36	122.05
genomic	25.84	1597.08
b-actin	27.32	643.56
1.00E+05	19.22	100000
1.00E+05	19.33	100000
1.00E+04	22.48	10000
1.00E+04	22.95	10000
1.00E+03	26.19	1000
1.00E+03	26.37	1000
1.00E+02	31.23	100
1.00E+02	30.48	100
1.00E+01	32.76	10
1.00E+01	35.02	10
1.00E−00	40	0
1.00E−00	40	0
NTC	40	0
NTC	40	0
				copies of
	Reg			mRNA		Fold
	number		Mean	detected/		Change in
Sample	(GSK		GOI	50 ng total		Disease
sbg102200MCTc	identifier)	Ct	copies	RNA	Sample	Population
colon normal GW98-167	21941	26.48	1723.59	3447.18	colon normal
colon tumor GW98-166	21940	26.06	2195.04	4390.08	colon tumor	1.27
colon normal GW98-178	22080	29.03	389.88	779.76	colon normal
colon tumor GW98-177	22060	27.39	1015.65	2031.30	colon tumor	2.61
colon normal GW98-561	23514	26.74	1478.76	2957.52	colon normal
colon tumor GW98-560	23513	26.37	1831.8	3663.60	colon tumor	1.24
colon normal GW98-894	24691	25.58	2918.02	5836.04	colon normal
colon tumor GW98-893	24690	25	4089.75	8179.50	colon tumor	1.40
lung normal GW98-3	20742	24.59	5183.31	10366.62	lung normal
lung tumor GW98-2	20741	24.94	4232.23	8464.46	lung tumor	−1.22
lung normal GW97-179	20677	25.73	2672.73	5345.46	lung normal
lung tumor GW97-178	20676	25.36	3307.37	6614.74	lung tumor	1.24
lung normal GW98-165	21922	26.13	2109.28	4218.56	lung normal
lung tumor GW98-164	21921	25.54	2973.82	5947.64	lung tumor	1.41
lung normal GW98-282	22584	27.08	1212.64	2425.28	lung normal
lung tumor GW98-281	22583	27.45	979.82	1959.64	lung tumor	−1.24
breast normal GW00-392	28750	26.68	1536.57	1536.57	breast normal
breast tumor GW00-391	28746	26.58	1626.58	3253.16	breast tumor	2.12
breast normal GW00-413	28798	31.71	81.9	81.90	breast normal
breast tumor GW00-412	28797	26.57	1632	3264.00	breast tumor	39.85
breast normal GW00-	27592-95	32.52	51.1	51.10	breast normal
235:238
breast tumor GW00-	27588-91	29.67	268.7	268.70	breast tumor	5.26
231:234
breast normal GW98-621	23656	26.48	1727.44	3454.88	breast normal
breast tumor GW98-620	23655	25.65	2793.6	5587.20	breast tumor	1.62
brain normal BB99-542	25507	28.62	494	988.00	brain normal
brain normal BB99-406	25509	29.45	304.68	609.36	brain normal
brain normal BB99-904	25546	30.08	211.25	422.50	brain normal
brain stage 5 ALZ BB99-	25502	28.75	458.64	917.28	brain stage 5	1.36
874					ALZ
brain stage 5 ALZ BB99-	25503	26.86	1383.71	2767.42	brain stage 5	4.11
887					ALZ
brain stage 5 ALZ BB99-	25504	28.02	702.59	1405.18	brain stage 5	2.09
862					ALZ
brain stage 5 ALZ BB99-	25542	29.57	284.31	568.62	brain stage 5	−1.18
927					ALZ
CT lung KC	normal	26.58	1624.29	3248.58	CT lung
lung 26 KC	normal	34.19	19.27	19.27	lung 26
lung 27 KC	normal	32.45	53.23	53.23	lung 27
lung 24 KC	COPD	33	38.6	38.60	lung 24	−21.75
lung 28 KC	COPD	32.24	59.95	59.95	lung 28	−14.01
lung 23 KC	COPD	32.87	41.63	41.63	lung 23	−20.17
lung 25 KC	normal	33.04	37.52	37.52	lung 25
asthmatic lung ODO3112	29321	30.13	205.46	205.46	asthmatic	−4.09
					lung
asthmatic lung ODO3433	29323	27.82	788.82	1577.64	asthmatic	1.88
					lung
asthmatic lung ODO3397	29322	25.17	3695.43	7390.86	asthmatic	8.80
					lung
asthmatic lung ODO4928	29325	27.6	894.3	1788.60	asthmatic	2.13
					lung
endo cells KC	control	28.2	633.43	633.43	endo cells
endo VEGF KC		28.86	429.51	429.51	endo VEGF	−1.47
endo bFGF KC		28.97	403.08	403.08	endo bFGF	−1.57
heart Clontech	normal	28.83	437.62	875.24	heart
heart (T-1) ischemic	29417	28.42	557.54	1115.08	heart T-1	1.27
heart (T-14) non-	29422	27.72	835.11	1670.22	heart T-14	1.91
obstructive DCM
heart (T-3399) DCM	29426	28.63	493.01	986.02	heart T-3399	1.13
adenoid GW99-269	26162	27	1269.75	2539.50	adenoid
tonsil GW98-280	22582	26.33	1876.29	3752.58	tonsil
T cells PC00314	28453	29.15	363.35	726.70	T cells
PBMNC		33.05	37.41	37.41	PBMNC
monocyte		31.49	92.84	185.68	monocyte
B cells PC00665	28455	26.5	1700.87	3401.74	B cells
dendritic cells 28441		24.2	6511.17	13022.34	dendritic cells
neutrophils	28440	27.01	1262.74	1262.74	neutrophils
eosinophils	28446	29.23	347.08	694.16	eosinophils
BM unstim		30.85	135.01	135.01	BM unstim
BM stim		28.68	478.5	478.50	BM stim	3.54
osteo dif		31.03	121.2	121.20	osteo dif	3.93
osteo undif		33.38	30.85	30.85	osteo undif
chondrocytes		26.63	1579.73	3949.33	chondrocytes
OA Synovium IP12/01	29462	29.11	371.98	371.98	OA
					Synovium
OA Synovium NP10/01	29461	29.45	304.55	609.10	OA
					Synovium
OA Synovium NP57/00	28464	27.83	784.87	1569.74	OA
					Synovium
RA Synovium NP03/01	28466	27.31	1063.77	2127.54	RA Synovium
RA Synovium NP71/00	28467	27.08	1217.21	2434.42	RA Synovium
RA Synovium NP45/00	28475	26.6	1606.41	3212.82	RA Synovium
OA bone (biobank)	29217	28.65	485.63	485.63	OA bone
					(biobank)
OA bone Sample 1	J. Emory	28.78	451.74	903.48	OA bone
OA bone Sample 2	J. Emory	28.27	607.15	1214.30	OA bone
Cartilage (pool)	Normal	29.42	310.76	621.52	Cartilage
					(pool)
Cartilage (pool)	OA	30.09	209.7	419.40	Cartilage	−1.48
					(pool)
PBL unifected	28441	23.85	7997.03	15994.06	PBL
					unifected
PBL HIV IIIB	28442	24.85	4447.34	8894.68	PBL HIV	−1.80
					IIIB
MRC5 uninfected (100%)	29158	27.02	1258.46	2516.92	MRC5
					uninfected
					(100%)
MRC5 HSV strain F	29178	29.6	278.84	557.68	MRC5 HSV	−4.51
					strain F
W12 cells	29179	27.21	1122.77	2245.54	W12 cells
Keratinocytes	29180	25.64	2815.12	5630.24	Keratinocytes
B-actin control		27.78	807.72
genomic		27.04	1246.22
1.00E+05		19.69	100000
1.00E+05		20.01	100000
1.00E+04		23.15	10000
1.00E+04		23.2	10000
1.00E+03		27.02	1000
1.00E+03		26.76	1000
1.00E+02		31.45	100
1.00E+02		32.39	100
1.00E+01		35.72	10
1.00E+01		34.74	10
1.00E−00		40	0
1.00E−00		40	0
NTC		40	0

[0251] Gene Name sbg102200MCTc

                  Fold Change in Disease
                  Population Relative to
Disease tissues   Normal
colon tumor       1.27
colon tumor       2.61
colon tumor       1.24
colon tumor       1.40
lung tumor        −1.22
lung tumor        1.24
lung tumor        1.41
lung tumor        −1.24
breast tumor      2.12
breast tumor      39.85
breast tumor      5.26
breast tumor      1.62
brain stage 5 ALZ 1.36
brain stage 5 ALZ 4.11
brain stage 5 ALZ 2.09
brain stage 5 ALZ −1.18
lung 24           −21.75
lung 28           −14.01
lung 23           −20.17
asthmatic lung    −4.09
asthmatic lung    1.88
asthmatic lung    8.80
asthmatic lung    2.13
endo VEGF         −1.47
endo bFGF         −1.57
heart T-1         1.27
heart T-14        1.91
heart T-3399      1.13
BM stim           3.54
osteo dif         3.93
Cartilage (pool)  −1.48
PBL HIV IIIB      −1.80
MRC5 HSV strain F −4.51

[0252] Gene Name sbg18525LRRb, SBhACRP30c, and sbg123493SLITc

[0253] Quantitative, tissue-specific, mRNA expression patterns of these three genes were measured using SYBR-Green Quantitative PCR (Applied Biosystems, Foster City, Calif.; see Schmittgen T. D. et al., Analytical Biochemistry 285: 194-204, 2000) or TaqMan PCR (Perkin Elmer, see Lie et al. Current Opinion in Biotechnology 9:43-48, 1998; Gibson et al., Genome Methods 6:995-1001, 1996) and human cDNAs prepared from various human tissues. Gene-specific PCR primers were designed using the first nucleic acid sequence listed in the Sequence List for each gene. Results are presented as the number of copies of each specific gene's mRNA detected in 1 ng mRNA pool from each tissue. Two replicate mRNA measurements were made from each tissue RNA.

	Tissue-Specific mRNA Expression
	(copies per ng mRNA; avg. ± range for 2 data points per tissue)
						Skeletal		Spleen/
Gene Name	Brain	Heart	Lung	Liver	Kidney	muscle	Intestine	lymph	Placenta	Testis
sbg18525LRRb	4290 ±	367 ±	47 ±	7 ±	263 ±	69 ±	401 ±	39 ±	119 ±	307 ±
	157	6	4	0	10	7	62	3	17	1
SBhACRP30c	10751 ±	7443 ±	9900 ±	6463 ±	8530 ±	7638 ±	6040 ±	8912 ±	8931 ±	8098 ±
	954	294	780	45	225	405	438	1021	617	612
sbg123493SLITc	9 ±	70 ±	13 ±	1 ±	41 ±	132 ±	6 ±	5 ±	9 ±	959 ±
	3	31	3	1	16	21	2	10	4	80

[0254]

TABLE V
Additional diseases based on mRNA expression in specific tissues
Tissue Exp. Additional Diseases
Brain       Neurological and psychiatric diseases,
            including Alzheimers, parasupranuclear
            palsey, Huntington's disease, myotonic
            dystrophy, anorexia, depression,
            schizophrenia, headache, amnesias, anxiety
            disorders, sleep disorders, multiple
            sclerosis
Heart       Cardiovascular diseases, including
            congestive heart failure, dilated
            cardiomyopathy, cardiac arrhythmias,
            Hodgson's Disease, myocardial
            infarction, cardiac arrhythmias
Lung        Respiratory diseases, including asthma,
            Chronic Obstructive Pulmonary
            Disease, cystic fibrosis, acute bronchitis,
            adult respiratory distress syndrome
Liver       Dyslipidemia, hypercholesterolemia,
            hypertriglyceridemia, cirrhosis, hepatic
            encephalopathy, fatty hepatocirrhosis,
            viral and nonviral hepatitis, Type II
            Diabetes Mellitis, impaired glucose tolerance
Kidney      Renal diseases, including acute and chronic
            renal failure, acute tubular necrosis,
            cystinuria, Fanconi's Syndrome,
            glomerulonephritis, renal cell carcinoma,
            renovascular hypertension
Skeletal    Eulenburg's Disease, hypoglycemia, obesity,
muscle      tendinitis, periodic paralyses,
            malignant hyperthermia, paramyotonia congenita,
            myotonia congenita
Intestine   Gastrointestinal diseases, including Myotonia
            congenita, Ileus, Intestinal
            Obstruction, Tropical Sprue, Pseudomembranous
            Enterocolitis
Spleen/     Lymphangiectasia, hypersplenism, angiomas,
lymph       ankylosing spondylitis, Hodgkin's
            Disease, macroglobulinemia, malignant lymphomas,
            rheumatoid arthritis
Placenta    Choriocarcinoma, hydatidiform mole,
            placenta previa
Testis      Testicular cancer, male reproductive diseases,
            including low testosterone and male infertility
Pancreas    Diabetic ketoacidosis, Type 1 & 2 diabetes,
            obesity, impaired glucose tolerance

[0255]

Claims

1. An isolated polypeptide selected from the group consisting of: (a) an isolated polypeptide encoded by a polynucleotide comprising a sequence set forth in Table I; (b) an isolated polypeptide comprising a polypeptide sequence set forth in Table I; and (c) a polypeptide sequence of a gene set forth in Table I.

2. An isolated polynucleotide selected from the group consisting of: (a) an isolated polynucleotide comprising a polynucleotide sequence set forth in Table I; (b) an isolated polynucleotide of a gene set forth in Table I; (c) an isolated polynucleotide comprising a polynucleotide sequence encoding a polypeptide set forth in Table I; (d) an isolated polynucleotide encoding a polypeptide set forth in Table I; (e) a polynucleotide which is an RNA equivalent of the polynucleotide of (a) to (d); or a polynucleotide sequence complementary to said isolated polynucleotide.

3. An expression vector comprising a polynucleotide capable of producing a polypeptide of claim 1 when said expression vector is present in a compatible host cell.

4. A process for producing a recombinant host cell which comprises the step of introducing an expression vector comprising a polynucleotide capable of producing a polypeptide of claim 1 into a cell such that the host cell, under appropriate culture conditions, produces said polypeptide.

5. A recombinant host cell produced by the process of claim 4.

6. A membrane of a recombinant host cell of claim 5 expressing said polypeptide.

7. A process for producing a polypeptide which comprises culturing a host cell of claim 5 under conditions sufficient for the production of said polypeptide and recovering said polypeptide from the culture.