Claims
- 1. A substantially pure polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:6, wherein the polypeptide has a G protein-coupled receptor protein activity.
- 2. The polypeptide of claim 1, wherein the amino acid sequence is at least 80% identical to SEQ ID NO:6.
- 3. The polypeptide of claim 1, wherein the amino acid sequence is at least 90% identical to SEQ ID NO:6.
- 4. A substantially pure polypeptide, the sequence of which consists of SEQ ID NO:6.
- 5. A substantially pure polypeptide, the sequence of which consists of SEQ ID NO:6 with up to 30 conservative amino acid substitutions, deletions or insertions, wherein the polypeptide has a G protein-coupled receptor protein activity.
- 6. A substantially pure polypeptide comprising the sequence of SEQ ID NO:6, or a fragment thereof that (a) has a G-protein receptor coupled protein activity or (b) is immunogenic.
- 7. A substantially pure polypeptide encoded by a nucleic acid that hybridizes under high stringency conditions to the sequence of SEQ ID NO:5, wherein the polypeptide has a G protein-coupled receptor protein activity.
- 8. An isolated nucleic acid encoding the polypeptide of claim 1.
- 9. An isolated nucleic acid encoding t he polypeptide of claim 4.
- 10. An isolated nucleic acid encoding the polypeptide of claim 5.
- 11. An isolated nucleic acid comprising a strand that hybridizes under high stringency conditions to the sequence of SEQ ID NO:5, or the complement of SEQ ID NO:5.
- 12. The isolated nucleic acid of claim 11, wherein the nucleic acid encodes a polypeptide having a G protein-coupled receptor protein activity.
- 13. The nucleic acid of claim 11, wherein the strand is at least 15 nucleotides in length.
- 14. An isolated nucleic acid comprising the sequence of SEQ ID NO:5.
- 15. An isolated nucleic acid comprising a sequence encoding the polypeptide of SEQ ID NO:6.
- 16. An antibody that specifically binds to the polypeptide consisting of SEQ ID NO:6.
- 17. A vector comprising the nucleic acid of claim 8.
- 18. A vector comprising the nucleic acid of claim 11.
- 19. A vector comprising the nucleic acid of claim 14.
- 20. A vector comprising the nucleic acid of claim 15.
- 21. A cultured host cell comprising the nucleic acid of claim 8.
- 22. A cultured host cell comprising the nucleic acid of claim 11.
- 23. A cultured host cell comprising the nucleic acid of claim 14.
- 24. A cultured host cell comprising the nucleic acid of claim 15.
- 25. An antibody that specifically binds to the polypeptide of claim 1.
- 26. A method of producing a polypeptide, the method comprising culturing the cultured host cell of claim 21 under conditions that permit expression of the polypeptide in the cell.
- 27. A method for identifying a compound that modulates a G protein-coupled receptor activity, comprising the steps of:
a) contacting a polypeptide of claim 1, or a cell transfected with a nucleic acid encoding the polypeptide of claim 1, with a test compound; and b) determining whether the test compound modulates a G protein-coupled receptor activity of the polypeptide or cell, thereby identifying a compound that modulates a G protein-coupled receptor activity.
- 28. A kit comprising the polypeptide of claim 1 and instructions for use in a method of screening.
- 29. A compound isolated by the method of claim 27.
- 30. A pharmaceutical composition comprising the compound of claim 29 as an active ingredient.
Priority Claims (1)
Number |
Date |
Country |
Kind |
11/82641 |
Mar 1999 |
JP |
|
Parent Case Info
[0001] This application is a continuation-in-part of PCT/JP00/01826, filed Mar. 24, 2000, and claims priority from Japanese Patent Application No. 11/82641, filed Mar. 24, 1999.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
PCT/JP00/01826 |
Mar 2000 |
US |
Child |
09963766 |
Sep 2001 |
US |