Claims
- 1. A recombinant DNA molecule comprising a nucleotide sequence encoding an TFNAR1 binding protein, wherein the protein has the amino acid sequence of SEQ ID NO: 2.
- 2. A recombinant DNA molecule in accordance with claim 1, further comprising a promoter operably-linked to said nucleotide sequence such that said promoter promotes the transcription of said nucleotide sequence when the recombinant DNA molecule is in an appropriate expression host.
- 3. A recombinant DNA molecule according to claim 2, wherein said promoter is a constitutive promoter in human cells.
- 4. A recombinant DNA molecule in accordance with claim 1, wherein said nucleotide sequence is SEQ ID NO: 1.
- 5. An expression vector comprising a recombinant nucleic acid molecule comprising a DNA molecule, or a corresponding RNA molecule, comprising the nucleotide sequence of SEQ ID NO: 1 in the anti-sense orientation, or comprising the nucleotide sequence of a fragment of SEQ ID NO: 1 in the anti-sense orientation, which fragment is of a size such that the transcription product of said nucleotide sequence hybridizes to sense mRNA encoding the interferon inducible IFNAR1 receptor binding protein of SEQ ID NO: 2, to form a non-translatable double-stranded RNA molecule.
- 6. An expression vector in accordance with claim 5, further comprising a promoter operably linked to said anti-sense nucleotide sequence.
- 7. An expression vector according to claim 6, wherein said nucleotide sequence of SEQ ID NO: 1 or fragment thereof, when in the sense orientation, includes the initiation codon at the 5′-end of SEQ ID NO: 1.
- 8. A recombinant DNA molecule according to claim 2, wherein said promoter is an interferon-inducible promoter.
- 9. A recombinant DNA molecule according to claim 1, which is an expression vector.
- 10. A host cell which includes an expression vector in accordance with claim 9.
- 11. An IFNAR1-binding protein having the sequence of SEQ ID NO: 2.
- 12. A method for producing an IFNAR1 receptor binding protein, comprising culturing a host cell transfected with a vector containing the DNA molecule of claim 1, and isolating the protein expressed thereby.
- 13. An RNA molecule which corresponds to a DNA molecule comprising the nucleotide sequence of SEQ ID NO: 1 in the anti-sense orientation, or comprising the nucleotide sequence of a fragment of SEQ ID NO: 1 in the antisense orientation, which fragment is of a size such that the transcription product of said nucleotide sequence hybridizes to sense mRNA encoding the interferon inducible IFNAR1 receptor binding protein of SEQ ID NO: 2, to form a non-translatable double-stranded RNA molecule.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation of U.S. application Ser. No. 09/341,650, filed Oct. 8, 1999, which is the national stage under 35 U.S.C. 371 of PCT/US98/00671, filed Jan. 15, 1998, which international application claims the benefit under 35 U.S.C. §119(e) of U.S. provisional application No. 60/035,636, filed Jan. 15, 1997, now abandoned.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60035636 |
Jan 1997 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09341640 |
Oct 1999 |
US |
Child |
10309280 |
Dec 2002 |
US |