Claims
- 1. A method for synthesizing an implantable article, comprising the steps of:
contacting a biocompatible substrate with a supersaturated calcifying solution under suitable conditions, wherein the calcifying solution comprises an effective amount of an amelogenin-type protein, and wherein said protein is incorporated into an enamel-like biomaterial which forms on the substrate.
- 2. The method of claim 1,
wherein the biocompatible substrate comprises one or more materials selected from the group consisting of titanium, titanium alloy, tantalum, tantalum alloy, stainless steel, cobalt chromium alloy, ultra high molecular weight polyethylene, hydroxyapatite, Bioglass and Glass Ceramic A-W.
- 3. The method of claim 1,
wherein the substrate is a metal coated with a nanometer-scaled porous oxide layer.
- 4. The method of claim 1,
wherein the substrate has been etched.
- 5. The method of claim 4,
wherein said etching comprises the steps of contacting the substrate with an effective amount of an acid and an effective amount of an oxidizing agent.
- 6. The method of claim 1,
wherein the calcifying solution comprises calcium phosphate and a buffering agent which maintains an approximately neutral pH.
- 7. The method of claim 6,
wherein the suitable conditions comprise contacting at approximately 20 to approximately 40 degrees Celsius.
- 8. The method of claim 6,
wherein the suitable conditions comprise adjusting the ionic strength of said calcifying solution to approximately 100 to 200 mM.
- 9. The method of claim 1,
where the amelogenin-type protein is a protein having the biological function of rM179.
- 10. The method of claim 9,
wherein the amelogenin-type protein comprises the sequence shown in SEQ ID No. 1.
- 11. The method of claim 1,
wherein the effective amount of the amelogenin-type protein is a concentration greater than approximately 12.5 μg/ml.
- 12. The method of claim 1,
wherein the effective amount of the amelogenin-type protein is a concentration greater than approximately 100 μg/ml.
- 13. The method of claim 1,
wherein the suitable conditions comprise contating the substrate with the supersaturated calcifying solution for a period time equal to or greater than approximately one hour.
- 14. The method of claim 11,
wherein the suitable conditions comprise contacting the supersaturated calcifying solution for a period of time equal to or greater than approximately 24 hours.
- 15. The method of claim 1, further comprising:
contacting the substrate with an effective amount of a first supersaturated calcifying solution substantially free of any amelogenin-type protein under suitable conditions prior to contacting the substrate with the second supersaturated calcifying solution comprising the amelogenin-type protein.
- 16. The method of claims 1 or 15,
wherein the calcifying solution further comprises an effective amount of a therapeutic agent.
- 17. The method of claim 16,
wherein the therapeutic agent is selected from the group consisting of an antibiotic, a, growth factor and an anti-inflammatory agent.
- 18. The method of claims 1 or 15,
wherein the suitable conditions comprise contacting the substrate with the solution containing the amelogenin-type protein until the desired growth of the enamel-like biomaterial is achieved.
- 19. The method of claim 18,
wherein the desired growth of the enamel-like biomaterial includes the incorporation of between 1×10−4% and 10% w/w amylogenin-type protein into the enamel-like biomaterial.
- 20. The method of claim 18,
wherein the desired growth of the enamel-like biomaterial includes the incorporation of between 1×10−3% and 1% w/w amylogenin-type protein into the enamel-like biomaterial.
- 21. The method of claim 18,
wherein the desired growth of the enamel-like biomaterial comprises submicron-sized bundles of elongated apatite crystals with an average aspect ratio of at least two.
- 22. A method for modifying the growth of apatite crystals onto a biocompatible substrate comprising:
adding an effective concentration of an amelogenin-type protein to a supersaturated calcifying solution and contacting the substrate with the supersaturated calcifying solution under suitable conditions until the desired growth of crystals is achieved.
- 23. The method of claim 22,
wherein the modification of crystals is a reduction in crystal size and an increase in the average aspect ratio (1/w) of the crystals to approximately two or greater.
- 24. A biocompatible implant produced by the method of claims 1 or 15.
- 25. A biocompatible implant produced by the method of claim 18.
- 26. The method of claims 1 or 15,
wherein the amelogenin-type protein is selected from the group consisting of TRAP, LRAP, full-length mouse amelogenin M180, rM179, rM166 and p172.
- 27. An implantable article, comprising:
a biocompatible substrate; and an enamel-like surface coating chemically bonded to at least a portion of the substrate, the coating comprising apatite crystals and an amelogenin-type protein, wherein said crystals are less than a 1 μm in length and have on average an aspect ratio of approximately two or greater.
- 28. The article of claim 27,
wherein the coating comprises ions selected from the group consisting of calcium, phosphate, sodium, sulfate, magnesium, chlorine, silicate, and mixtures thereof.
- 29. The article of claim 27, further comprising an effective amount of a therapeutic agent.
- 30. A method for enhancing bone ingrowth or soft tissue attachment comprising implanting the article of claim 27 onto a bone surface or soft tissue.
- 31. A method for delivery of a therapeutic agent comprising:
implanting the article of claim 25 onto a bone surface or soft tissue.
CROSS REFERENCE TO RELATED APPLICATION
[0001] This application claims priority under 35 U.S.C. §119(e) of U.S. Provisional Application Serial No. 60/358,157, filed Feb. 20, 2002, entitled “Novel Materials for Dental and Biomedical Application.” The application is incorporated herein by this reference.
STATEMENT AS TO INVENTION RIGHTS UNDER FEDERALLY SPONSORED RESEARCH
[0002] This work was supported by NICDR research grants DE12350 and DE13414. The United States government may have certain rights in the inventions disclosed herein.
Provisional Applications (1)
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Number |
Date |
Country |
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60358157 |
Feb 2002 |
US |