The present invention relates to a method for extraction of Oxyresveratrol from Artocarpus hirsutus (A. hirsutus).
Oxyresveratrol is a hydroxyl-substituted stilbene found in the roots, leaves, stem and fruit of many widely distributed plants including Moraceae, Liliaceae, Gnetaceae, etc. The biological activity of this compound is well documented in the following scientific articles;
It is also reported for its modest inhibition of HIV (Likhitwitayawuid K, Sritularak B, Benchanak K, Lipipun V, Mathew J, Schinazi R F, Phenolics with antiviral activity from Millettia erythrocalys and Artocarpus lakoocha, Nat Prod Res. 2005 February; 19(2):177-82).
Oxyresveratrol has been isolated from several Artocarpus species (A. lakoocha Roxb.; A. champlasha Roxb.; A. champlasha Roxb.; A. heterophyllus; A. gomezianus Wall.). (Nasapon Povichit, Ampai Phrutivorapongkul, Maitree Suttajit and Pimporn Leelapornpisid, Antiglycation and antioxidant activities of Oxyresveratrol extracted from the heartwood of Artocarpus lakoocha Roxb., Maejo Int. J. Sci. Technol. 2010, 4(03), 454-461) using chromatographic techniques like HPLC, Column chromatography etc. While these techniques are expensive and time consuming, a simple inexpensive and reliable method of isolation is technically desired for the large scale industrial production of Oxyresveratrol. The present invention solves such technical problems by disclosing a simple solvent-solvent purification method of Oxyresveratrol from A. hirsutus with high recovery.
It is the principle objective of the invention to disclose a simple, commercially viable method for the extraction of Oxyresveratrol from Artocarpus hirsutus.
The present invention fulfils the aforesaid objectives and provides further related advantages.
Disclosed is method for extraction of Oxyresveratrol from A. hirsutus. More specifically, the invention discloses a novel method for extraction of Oxyresveratrol from A. hirsutus using a simple solvent-solvent purification method.
In the most preferred embodiment, the present invention discloses a novel method for extraction of Oxyresveratrol from Artocarpus hirsutus, said method comprising steps of:
Other features and advantages of the present invention will become apparent from the following more detailed description, taken in conjunction with the accompanying images, which illustrate, by way of example, the principle of the invention.
Preparation of the Extract
Artocarpus hirsutus wood was collected and cut into small pieces, dried in shade. The dried material was pulverised to a coarse powder and stored in an air tight container. The powdered material was extracted completely with soaked volume of hot ethanol (3 volumes×3 times). The ethanolic extracts were separated by filtration and collected in a clean container. The combined extracts were concentrated to thick paste under vacuum at 50-55° C. and dried in vacuum tray drier to get powder. The powdered extract was collected and stored at room temperature in air tight container.
Isolation and Characterization of Active Compound
The powdered ethanolic extract from A. hirsutus wood was redissolved in a small amount (2 volumes) of ethanol and poured into 10 volumes of water. The material was filtered to separate the water soluble and water insoluble fraction. The water soluble fraction was then extracted with ethyl acetate (1 volume) and the ethyl acetate layer was dried completely to get the powder. The powder obtained from ethyl acetate fraction was then poured into water (4 volumes) and stirred at 80-90° C. for 8 h. After cooling at room temperature, the solution was filtered and the crystallised solid material was dried under vacuum at 65 -70° C. This filtered crystallised material was characterized as Oxyresveratrol from its 1H and 13C NMR spectra as well as from its LCMS spectrum and was comparable with the reported values. The isolated Oxyresveratrol shows the following values: C14H12O4. m. p.: 191-194° C. Color: pale brown fine powder, APCI-MS m/z 245.00 (M+H+) and 245.05 (M-H−) (C14H12O4 requires 244.2426). Purity of the compound was further verified by HPLC.
While the invention has been described with reference to a preferred embodiment, it is to be clearly understood by those skilled in the art that the invention is not limited thereto. Rather, the scope of the invention is to be interpreted only in conjunction with the appended claims.
This is National Phase Application of PCT application no. PCT/US 16/68161 filed on 22 Dec. 2016.
Filing Document | Filing Date | Country | Kind |
---|---|---|---|
PCT/US16/68161 | 12/22/2016 | WO | 00 |