Claims
- 1-7. (Canceled)
- 8. An isolated microtubule motor protein, wherein the protein has greater than 70% amino acid sequence identity to SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm.
- 9-18. (Canceled)
- 19. A method for screening for modulators of HsKip3a, the method comprising:
(a) providing a HsKip3a protein that has (i) an amino acid sequence that has greater than 90% sequence identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, and (ii) microtubule stimulated ATPase activity; and (b) contacting the HsKip3a protein with a candidate agent that is present at a test concentration and with the candidate agent that is present at a control concentration; and (c) assaying for the level of HsKip3a activity at the test and control concentrations, wherein
the HsKip3a activity is a HsKip3a binding activity or ATPase activity, and a change in HsKip3a activity between the test and control concentration indicates that the candidate agent is a modulator of HsKip3a.
- 20. The method of claim 19, wherein the screening occurs in a multi-well plate as part of a high-throughput screen.
- 21. The method of claim 19, wherein the HsKip3a protein comprises a HsKip3a motor domain, the motor domain comprising amino acids 5-342 or 26-354 of SEQ ID NO:2.
- 22. The method of claim 19, wherein the HsKip3a protein has greater than 95% sequence identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.
- 23. The method of claim 22, wherein the HsKip 3a protein has greater than 98% sequence identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4.
- 24. The method of claim 19, wherein the assay conducted at the control concentration is conducted in the absence of inhibitor.
- 25. The method of claim 19, wherein assaying comprises detecting ADP formation.
- 26. The method of claim 19, wherein assaying comprises detecting phosphate formation.
- 27. A method for screening for modulators of HsKip3a, the method comprising:
(a) providing a HsKip3a protein that has: (i) an amino acid sequence that has greater than 90% sequence identity to the amino acid sequence of SEQ ID NO:2 or SEQ ID NO:4 as measured using a sequence comparison algorithm, and (ii) microtubule stimulated ATPase activity; and (b) contacting HsKip3a with a candidate agent and determining whether the candidate agent modulates the ATPase activity of HsKip3a.
CROSS-REFERENCE TO RELATED APPLICATION
[0001] The present application is a continuation-in-part of U.S. Ser. No. 09/594,655 filed Jun. 15, 2000, which is incorporated by reference in its entirety for all purposes.
Divisions (1)
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Number |
Date |
Country |
Parent |
09883096 |
Jun 2001 |
US |
Child |
10723147 |
Nov 2003 |
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
09594655 |
Jun 2000 |
US |
Child |
09883096 |
Jun 2001 |
US |