Patent Application
20160207930
The present invention relates to novel heterocyclic compounds which are neurokinin 1 receptor antagonists, to intermediates for the preparation of said compounds, to their use in therapy such as in the prophylaxis or treatment of pruritic dermal diseases or conditions, and to pharmaceutical compositions comprising said compounds.
Pruritus is a common symptom of skin diseases as well as a sign of an underlying systemic pathology. Pruritus is an unpleasant sensation in the skin that provokes a desire to scracth and may be acute (of short duration) such as the reaction to an insect bite or chronic (lasting for more than 6 weeks) such as in many inflammatory skin diseases. It is well known that patients with inflammatory skin diseases perceive pruritus as seriously compromising their quality of life.
Pruritus is mediated via free nerve endings of non-myelinated C-type nerve fibres in epidermis. These have been found to express neuropeptides, and the epidermal keratinocytes produce neuropeptides, receptors for neuropeptides, nerve growth factor, vanilloid receptors proteinase-activated receptor type 2 (PAR2) and voltage-gated ATP channels (M W Greaves, Curr. Allergy Asthma Rep. 10, 2010, pp. 236-242).
Neuropeptides suchs as Substance P have been shown to increase the production and release of nerve growth factor from cultured keratinocytes, suggesting that interactions between the immune system and the nervous system are important in the development of inflammation including pruritus. Inflammatory skin diseases such as atopic dermatitis, contact dermatitis, psoriasis and urticaria are associated with increased production of cytokines, neurotrophins and neuropeptides that may exacerbate the pruritus (U. Raap et al., Curr. Opin. Allergy Clin. Immunol. 11, 2011, pp. 420-427). While neuropeptides such as Substance P are not considered crucial for the pathogenesis of inflammatory skin diseases such as atopic dermatitis, they do play an important role in the development and severity of the condition, for instance by provoking the itch-scratch cycle where scratching exacerbates the inflammatory symptoms of atopic dermatitis (J. Salomon and E. Baran, JEADV 22, 2008, pp. 223-228). Furthermore, an increase of dermal nerves and upregulation of receptors for neuropeptides, e.g. the neurokinin 1 receptor has been found in skin from psoriasis patients with pruritus as opposed to skin from psoriasis patients without pruritus (S-E. Chang et al., Br. J. Dermatol. 156, 2007, pp. 1272-1277).
It has also been found that other cell types resident in skin release mediators of pruritus. Thus, mast cells contain large amounts of histamine that are released on activation of the cells and induce pruritus by targeting histamine H1 receptors on nerve endings. Eosinophils which infiltrate inflamed skin in atopic dermatitis, urticaria and contact dermatitis produce and release neurotrophins such as nerve growth factor.
Current therapeutic treatments of pruritus include both topical and systemic medicaments. Topical treatments include emollients and barrier creams that are believed to act by improving the barrier function of the skin, corticosteroids that do not appear to be antipruritic in themselves but act by relieving the attendant skin inflammation. This may also be the case for calcineurin inhibitors such as tacrolimus which has been shown to reduce pruritus in atopic dermatitis patients. Histamine H1 antagonists have also shown effect against pruritus in atopic dermatitis patients, but exhibits systemic side effects in the form of drowsiness in a significant number of patients. Local anesthetics such as lidocain have been found to exhibit anti-pruritic properties (Patel and Yosipovitch, Expert. Opin. Pharmacother. 11(10), 2010, 1673-1682).
Systemic therapy of pruritus include oral antihistamines, antidepressants, neuroleptics and immunosuppressants such as cyclosporin. A neurokinin-1 receptor antagonist, aprepitant, which has been developed as an oral antiemetic drug for use to counteract the nausea and vomiting caused by chemotherapy or post surgery, has been found to relieve pruritus in patients suffering from Sézary syndrome by oral administration (Duval and Dubertret, New Engl. J. Med. 361, 2009, pp. 1415-1416) and in patients with atopic diathesis, prurigo nodularis and systemic pruritus (S. Stander et al., Plos One, 2010, p. 5).
WO01/25219 discloses piperazine derivatives which are antagonists of tachykinins, including substance P and other neurokinins.
WO02/32867 discloses piperidine derivatives which are antagonists of tachykinins, including substance P and other neurokinins
WO02/081457 discloses 1,4-diazepane-1-carboxylic acid derivatives process for their preparation and their use as tachykinin antagonists
WO2009002770 discloses 6.5 pyrrolopiperidine tachykinin receptor antagonists.
WO2013124286 discloses Novel Neurokinin 1 Receptor Antagonists.
As many of the antipruritic treatments available at present have side-effects that may limit their use, and as many dermal conditions are preferentially treated with topical medications, especially when the conditions is of mild to moderate severity, there is a continued need to develop neurokinin 1 receptor (NK1R) antagonists which are effective in the treatment of itch on topical application, but which have reduced systemic effects on the central nervous system.
In one aspect, the present invention relates to compounds of general formula A
wherein
R1 and R2 independently are selected from the group consisting of (C1-4)alkyl, (C1-4)haloalkyl, (C1-4)alkoxy, CD3 or halogen;
R3 is selected from the group consisting of hydrogen, (C1-4)alkyl, (C1-4)haloalkyl and (C1-4)hydroxyalkyl;
R4 is selected from the group consisting of phenyl, 5-membered heteroaryl and 6-membered heteroaryl, said phenyl and 6-membered heteroaryl being substituted with one ore more substituents independently selected from R7, and said 5-membered heteroaryl optionally being substituted with one ore more substituents independently selected from R7;
R5 and R6 are independently selected from the group consisting of hydrogen, (C1-4)alkyl, (C1-4)hydroxyalkyl and (C1-4)haloalkyl;
R7 is selected from the group consisting of halogen, hydroxy, (C1-4)alkyl, (C1-4)alkoxy, aryloxy, aryl(C1-4)alkoxy, cyano, (C1-4)haloalkyl or (C1-4)hydroxyalkyl, wherein two adjacent (C1-4)alkyl's, together with the phenyl, 5-membered heteroaryl or 6-membered heteroaryl to which they are attached, may optionally form a 4-7 membered aliphatic ring;
X and Y are independently selected from the group consisting of CH and N;
or a pharmaceutically acceptable salt or solvate thereof;
with the proviso that when both X and Y are CH, R4 is not 2-methylphenyl.
In another aspect the present invention relates to a compound according to general formula A for use in therapy.
In another aspect the present invention relates to a compound according to general formula A for use in the prevention, treatment or amelioration of pruritic skin conditions, e.g. acute pruritus in any condition; chronic pruritus on diseased skin, such as inflammatory, infectious, or autoimmune cutaneous diseases, genodermatoses, drug reactions, dermatoses of pregnancy and skin lymphomas, prurigo, lichen planus, atopic dermatitis, eczema, contact dermatitis, allergic dermatitis, nummular dermatitis, lichen simplex, psoriasis, Sézary syndrome, cutaneous lymphomas, bullous pemphigoid, alopecia areata, scabies, vitiligo, urticaria and drug-induced pruritus; pruritic diseases on non-diseased skin of systemic, neurological or psychosomatic/psychiatric origin, including endocrine and metabolic disorders, infections, haematological and lymphoproliferative diseases, solid neoplasms and drug-induced pruritus; mastocytosis; pruritus of unknown cause; pruritus with chronic secondary scratch lesions, such as prurigo nodularis, and all types of prurigo; or any other dermal disease or condition characterized by pruritus.
In another aspect the present invention relates to a pharmaceutical composition comprising, as a therapeutically active ingredient, a compound according to general formula A and a pharmaceutically acceptable carrier or vehicle.
In another aspect the present invention relates to intermediates for the preparation of a compound according to general formula A, selected from the list consisting of
The compounds of formula A have been found to act as potent antagonists of NK1R and are therefore potentially useful in the treatment of any disease or condition where Substance P is involved in the disease pathology, in particular in the prevention, treatment or amelioration of a pruritic dermal disease or condition.
It has surprisingly been found that some compounds of formula A exhibit a high metabolic clearance, i.e. are quickly degraded upon systemic administration, which makes them uniquely suitable for topical application with a favourable safety profile.
It has surprisingly been found that some compounds of general formula A exhibit a favourable ratio between the in vitro efficacy of the compound and the in vitro efficacy of metabolites of the compound, thus providing a favourable safety profile of the compounds.
It has surprisingly been found that some compounds of general formula A exhibit a mode of action indicating a competitive, non-surmountable antagonism, suggesting a slow dissociating antagonist-receptor complex.
It has surprisingly been found that some compounds of general formula A both exhibit a mode of action indicating a competitive, non-surmountable antagonism as well as exhibiting a favourable ratio between the in vitro efficacy of the compound and the in vitro efficacy of metabolites of the compound.
The term “alkyl” is intended to indicate a radical obtained when one hydrogen atom is removed from a hydrocarbon. Said alkyl may be branched or straight and may comprise 1-6, such as 1-4 carbon atoms, such as 1-3 carbon atoms, such as 1-2 carbon atoms, such as 2-3 carbon atoms. The term includes the subclasses normal alkyl (n-alkyl), secondary and tertiary alkyl, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec.-butyl, tert.-butyl, pentyl, isopentyl, neopentyl and hexyl.
The term “(C1-4)alkyl” is intended to indicate a radical obtained when one hydrogen atom is removed from a hydrocarbon. Said alkyl may be branched or straight and may comprise 1-4, such as 1-3 carbon atoms, such as 1-2 carbon atoms, such as 2-3 carbon atoms. The term includes the subclasses normal alkyl (n-alkyl), secondary and tertiary alkyl, such as methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec.-butyl and tert.-butyl.
The term “aryl” is intended to indicate a radical of aromatic carbocyclic rings comprising 6-10 carbon atoms, such as 6 carbon atoms. Representative examples of aryl include, but are not limited to phenyl and naphthyl.
The term “halogen” is intended to indicate a substituent from the 7th main group of the periodic table, such as fluoro, chloro, bromo and iodo.
The term “haloalkyl” is intended to indicate an alkyl radical as defined above substituted with one or more halogen atoms, e.g trifluoromethyl, difluoromethyl, fluoromethyl or trifluoroethyl.
The term “(C1-4)haloalkyl” is intended to indicate an alkyl radical as defined above wherein the alkyl group consists of 1-4 carbon atoms and wherein the alkyl group is substituted with one or more halogen atoms, e.g trifluoromethyl, difluoromethyl, fluoromethyl or trifluoroethyl.
The term “heteroaryl” is intended to indicate radicals of monocyclic heteroaromatic rings comprising 5- or 6-membered rings which contain from 1-5 carbon atoms and from 1-4 heteroatoms selected from oxygen, sulphur and nitrogen. The heteroaryl radical may be connected to the parent molecular moiety through a carbon atom or a nitrogen atom contained anywhere within the heteroaryl group. Representative examples of 5-membered heteroaryl groups include, but are not limited to, furanyl, imidazolyl, isothiazolyl, isoxazolyl, oxadiazolyl, oxazolyl, pyrazolyl, pyrrolyl, tetrazolyl, thiadiazolyl, thiazolyl, thienyl and triazolyl. Representative examples of 6-membered heteroaryl groups include, but are not limited to, pyrazinyl, pyridazinyl, pyridyl and pyrimidinyl.
The term “hydroxyalkyl” is intended to indicate an alkyl group as defined above substituted with one or more hydroxyl-groups, e.g. hydroxymethyl, hydroxyethyl, hydroxypropyl.
The term “(C1-4)hydroxyalkyl” is intended to indicate an alkyl group as defined above, wherein the alkyl group consists of 1-4 carbon atoms and wherein the alkyl group is substituted with one or more hydroxyl-groups, e.g. hydroxymethyl, hydroxyethyl, hydroxypropyl.
The term “alkoxy” is intended to indicate a radical of the formula —OR′, wherein R′ is alkyl as indicated above, e.g. methoxy, ethoxy, n-propoxy, isopropoxy, butoxy, etc.
The term “(C1-4)alkoxy” is intended to indicate a radical of the formula —OR′, wherein R′ is alkyl as indicated above and wherein the alkyl group consists of 1-4 carbon atoms, e.g. methoxy, ethoxy, n-propoxy, isopropoxy, butoxy, tert-butoxy.
The tem “aliphatic ring” is intended to indicate a non-aromatic ring. The aliphatic ring may contain unsaturated bonds.
The term “2-methylphenyl” is intended to indicate a radical of the formula
The term “oxo” is intended to indicate an oxygen atom which is connected to a carbon atom by a double bond, thereby forming a carbonyl group together with the carbon-atom to which it is connected.
The term “pharmaceutically acceptable salt” is intended to indicate salts prepared by reacting a compound of formula A or B with a suitable inorganic or organic acid, such as hydrochloric, hydrobromic, hydroiodic, sulfuric, nitric, phosphoric, formic, acetic, 2,2-dichloroaetic, adipic, ascorbic, L-aspartic, L-glutamic, galactaric, lactic, maleic, L-malic, phthalic, citric, propionic, benzoic, glutaric, gluconic, D-glucuronic, methanesulfonic, salicylic, succinic, malonic, tartaric, benzenesulfonic, ethane-1,2-disulfonic, 2-hydroxy ethanesulfonic acid, toluenesulfonic, sulfamic or fumaric acid.
The term “solvate” is intended to indicate a species formed by interaction between a compound, e.g. a compound of formula A, and a solvent, e.g. alcohol, glycerol or water, wherein said species are in a solid form. When water is the solvent, said species is referred to as a hydrate.
The term ‘non-surmountable antagonist’ or ‘insurmountable antagonist’ is intended to indicate an antagonist which produces shifts to the right of agonist concentration-response curves in the presence of increasing antagonist concentrations with depression of the maximal response to the agonist (ref. Kenakin, T. et. al., J. Pharm. Exp. Ther., (2006), 319, p. 710-723).
The term ‘surmountable antagonist’ is intended to indicate an antagonist which produces shifts to the right of agonist concentration-response curves in the presence of increasing antagonist concentrations with no concomitant diminution of the maximal response to the agonist. (ref. Kenakin, T. et. al., J. Pharm. Exp. Ther., (2006), 319, p. 710-723).
Chronic pruritus on diseased skin is intended to indicate pruritus in skin diseases which are accompanied by itch, such as inflammatory, infectious, or autoimmune cutaneous diseases, genodermatoses, drug reactions, dermatoses of pregnancy and skin lymphomas.
Genodermatoses are intended to include Darier's disease, Hailey-Hailey disease, ichthyoses, Sjögren-Larsson syndrome, EB pruriginosa.
Dermatoses of pregnancy are intended to include polymorphic eruption of pregnancy, pemphigoid gestationis, prurigo gestationis.
Skin lymphomas are intended to include cutaneous T-cell-lymphoma, cutaneous B-celllymphoma.
Pruritic diseases of systemic origin are intended to comprise diseases arising from diseases of organs other than the skin, such as liver, e.g. primary biliary cirrhosis; kidney, e.g. chronic renal failure; blood, e.g. Hodgkin's disease; and certain multifactorial, e.g. metabolic states or drugs.
Pruritic systemic endocrine and metabolic diseases are intended to comprise chronic renal failure, liver diseases with or without cholestasis, hyperthyroidism, malabsorption, perimenopausal pruritus.
Pruritic systemic infectious diseases are intended to comprise HIV-infection, helminthosis, Parasitosis.
Pruritic systemic haematological and lymphoproliferative diseases are intended to comprise iron deficiency, polycythaemia vera, Hodgkin's disease, Non-Hodgkin's lymphoma, plasmocytoma.
Chronic secondary scratch lesions are intended to indicate secondary acquired lesions induced by chronic scratching, such as for example lichen simplex chronicus, lichen Vidal, lichen amyloidosus, macular amyloidosus, and prurigo nodularis.
In an embodiment the present invention relates to compounds of general formula A(i)
wherein R1, R2, R3, R4, R5, R6, X and Y are as indicated above,
or a pharmaceutically acceptable salt or solvate thereof;
with the proviso that when both X and Y are CH, R4 is not 2-methylphenyl.
In an embodiment the present invention relates to compounds of general formula A(ii)
wherein R1, R2, R3, R4, R5, R6, X and Y are as indicated above,
or a pharmaceutically acceptable salt or solvate thereof;
with the proviso that when both X and Y are CH, R4 is not 2-methylphenyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R1 and R2 represent (C1-4)haloalkyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R1 represents (C1-4)haloalkyl and R2 represents (C1-4)alkyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R3 is selected from the group consisting of hydrogen and (C1-4)alkyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R5 and R6 independently are selected from the group consisting of hydrogen and (C1-4)hydroxyalkyl
In an embodiment the present invention relates to compounds of general formula A, wherein R7 is selected from the group consisting of halogen and (C1-4)alkyl.
In an embodiment the present invention relates to compounds of general formula A, wherein X and Y represent CH.
In an embodiment the present invention relates to compounds of general formula A, wherein R4 is phenyl, wherein said phenyl is substituted with two substituents independently selected from R7.
In an embodiment the present invention relates to compounds of general formula A, selected from the group consisting of
In an embodiment the present invention relates to compounds of general formula A, selected from the group consisting of
In an embodiment the present invention relates to compounds of general formula A, selected from the group consisting of
In an embodiment the present invention relates to compounds of general formula A, wherein R1 and R2 independently are selected from the group consisting of trifluoromethyl, methyl, ethyl, isopropyl and chloro.
In an embodiment the present invention relates to compounds of general formula A, wherein R1 represents trifluoromethyl and wherein R2 is selected from the group consisting of methyl, ethyl, isopropyl and chloro.
In an embodiment the present invention relates to compounds of general formula A, wherein R3 is selected from the group consisting of hydrogen and methyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R4 is selected from the group consisting of phenyl, pyridyl, thiophenyl, thiazolyl and pyrazolyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R5 and R6 independently are selected from the group consisting of hydrogen and 2-hydroxyethyl.
In an embodiment the present invention relates to compounds of general formula A, wherein R7 is selected from the group consisting of methyl, fluoro, methoxy, benzyloxy and hydroxy.
In an embodiment the present invention relates to compounds of general formula A, wherein X and Y represent CH.
In an embodiment the present invention relates to compounds of general formula A, wherein X represents N and Y represents CH.
In an embodiment the present invention relates to compounds of general formula A, wherein R4 is substituted with two substituents independently selected from R7.
In an embodiment the present invention relates to compounds of general formula A, wherein R4 is substituted with two adjacent substituents independently selected from (C1-4)alkyl, and wherein said substituents together with the phenyl, 5-membered or 6-membered heteroaryl to which they are attached form a 4-7 membered aliphatic ring. Representative examples of such bicyclic R4's include, but are not limited to indanyl and tetrahydronaphtyl.
In an embodiment the present invention relates to compounds of general formula A(iii),
wherein R1, R2, R3, R4, R5, R6, X and Y are as indicated above,
or a pharmaceutically acceptable salt or solvate thereof,
with the proviso that when both X and Y are CH, R4 is not 2-methylphenyl.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from 1-(2,4-dimethylphenyl)-7,7-bis(2-hydroxyethyl)-N-methyl-N—((R)-1-(3-methyl-5-(trifluoromethyl)phenyl)ethyl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide_or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-1-(2,3-dimethylphenyl)-7,7-bis(2-hydroxyethyl)-N-methyl-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-1-(2,3-dimethylphenyl)-N-methyl-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)-1-(2,3-dimethylphenyl)-N—((R)-1-(3-methyl-5-trifluoromethyl)phenyl)ethyl)-N-methyl-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-1-(3-methylpyridin-4-yl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-1-(4-methylpyridin-3-yl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1R,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-1-(3-methylpyridin-2-yl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from or (1S,8aS)-1-(2,3-dimethylphenyl)-N—((R)-1-(3-isopropyl-5-trifluoromethyl)phenyl)ethyl)-N-methyl-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide a pharmaceutically acceptable salt or solvate thereof.
In an embodiment the present invention relates to compounds of general formula A, the compounds being selected from (1S,8aS)-1-(2,3-dimethylphenyl)-N—((R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)ethyl)-N-methyl-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide or a pharmaceutically acceptable salt or solvate thereof.
The compounds of formula A may be obtained in crystalline form either directly by concentration from an organic solvent or by crystallisation or recrystallisation from an organic solvent or mixture of said solvent and a cosolvent that may be organic or inorganic, such as water. The crystals may be isolated in essentially solvent-free form or as a solvate, such as a hydrate. The invention covers all crystalline modifications and forms and also mixtures thereof.
Compounds of formula A may comprise asymmetrically substituted (chiral) carbon atoms which give rise to the existence of isomeric forms, e.g. enantiomers and possibly diastereomers. The present invention relates to all such isomers, either in pure form or as mixtures thereof (e.g. racemates). Pure stereoisomeric forms of the compounds and the intermediates of this invention may be obtained by the application of procedures known in the art. The various isomeric forms may be separated by physical separation methods such as selective crystallization and chromatographic techniques, e.g. liquid chromatography using chiral stationary phases. Enantiomers may be separated from each other by the selective crystallization of their diastereomeric salts with optically active acids. Alternatively, enantiomers may be separated by chromatographic techniques using chiral stationary phases. Said pure stereoisomeric forms may also be derived from the corresponding pure stereoisomeric forms of the appropriate starting materials, provided that the reaction occurs stereoselectively or stereospecifically. Preferably, if a specific stereoisomer is desired, said compound will be synthesized by stereoselective or stereospecific methods of preparation. These methods will advantageously employ chiral pure starting materials.
In an embodiment the present invention relates to intermediates for the preparation of a compound according to general formula A, selected from the list consisting of
In an embodiment the present invention relates to intermediates for the preparation of a compound according to general formula A, selected from the list consisting of
An embodiment of the invention is a compound according to general formula A for use in the prevention, treatment or amelioration of pruritic skin conditions, wherein the pruritic skin condition is selected from prurigo, lichen planus, atopic dermatitis, eczema, contact dermatitis, allergic dermatitis, nummular dermatitis, lichen simplex, psoriasis, Sézary syndrome, cutaneous lymphomas, urticaria, mastocytosis and pruritus with chronic secondary scratch lesions.
An embodiment of the invention is a pharmaceutical composition comprising, comprising as a therapeutically active ingredient, a compound according to general formula A and a pharmaceutically acceptable carrier or vehicle, together with one or more other therapeutically active compound(s).
An embodiment of the invention is a pharmaceutical composition comprising, comprising as a therapeutically active ingredient, a compound according to general formula A and a pharmaceutically acceptable carrier or vehicle, together with one or more other therapeutically active compound(s), suitable for topical administration.
An embodiment of the invention is a method of preventing, treating or ameliorating a condition involving pruritus of the skin, the method comprising applying, on the skin of a patient in need thereof, a therapeutically effective amount of a compound according to general formula A, optionally together with a pharmaceutically acceptable carrier or one or more excipients, optionally in combination with other therapeutically active compounds, wherein the condition is selected from the group consisting of acute pruritus in any condition; chronic pruritus on diseased skin, such as inflammatory, infectious, or autoimmune cutaneous diseases, genodermatoses, drug reactions, dermatoses of pregnancy and skin lymphomas, prurigo, lichen planus, atopic dermatitis, eczema, contact dermatitis, allergic dermatitis, nummular dermatitis, lichen simplex, psoriasis, Sézary syndrome, cutaneous lymphomas, bullous pemphigoid, alopecia areata, scabies, vitiligo, urticaria and drug-induced pruritus; pruritic diseases on non-diseased skin of systemic, neurological or psychosomatic/psychiatric origin, including endocrine and metabolic disorders, infections, haematological and lymphoproliferative diseases, solid neoplasms and drug-induced pruritus; mastocytosis; pruritus of unknown cause; pruritus with chronic secondary scratch lesions, such as prurigo nodularis, and all types of prurigo; or any other dermal disease or condition characterized by pruritus.
An embodiment of the invention relates to the use of a compound according to general formula A in the manufacture of a medicament for the prevention, treatment or amelioration of pruritic skin conditions, e.g. acute pruritus in any condition; chronic pruritus on diseased skin, such as inflammatory, infectious, or autoimmune cutaneous diseases, genodermatoses, drug reactions, dermatoses of pregnancy and skin lymphomas, prurigo, lichen planus, atopic dermatitis, eczema, contact dermatitis, allergic dermatitis, nummular dermatitis, lichen simplex, psoriasis, Sézary syndrome, cutaneous lymphomas, bullous pemphigoid, alopecia areata, scabies, vitiligo, urticaria and drug-induced pruritus; pruritic diseases on non-diseased skin of systemic, neurological or psychosomatic/psychiatric origin, including endocrine and metabolic disorders, infections, haematological and lymphoproliferative diseases, solid neoplasms and drug-induced pruritus; mastocytosis; pruritus of unknown cause; pruritus with chronic secondary scratch lesions, such as prurigo nodularis, and all types of prurigo; or any other dermal disease or condition characterized by pruritus.
An embodiment of the invention relates to a pharmaceutical composition comprising, as a therapeutically active ingredient, a compound according to general formula A and a pharmaceutically acceptable carrier or vehicle.
An embodiment of the invention relates to a pharmaceutical composition suitable for topical administration comprising, comprising as a therapeutically active ingredient, a compound according to general formula A and a pharmaceutically acceptable carrier or vehicle.
An embodiment of the invention relates to a method of preventing, treating or ameliorating a condition involving pruritus of the skin, the method comprising applying, on the skin of a patient in need thereof, a therapeutically effective amount of a compound according to general formula A.
Besides being useful for human treatment, the compounds of the present invention may also be useful for veterinary treatment of animals including mammals such as horses, cattle, sheep, pigs, dogs, and cats.
For use in therapy, compounds of the present invention are typically in the form of a pharmaceutical composition. The invention therefore relates to a pharmaceutical composition comprising a compound of formula A, optionally together with one or more other therapeutically active compound(s), together with a pharmaceutically acceptable excipient or vehicle. The excipient must be “acceptable” in the sense of being compatible with the other ingredients of the composition and not deleterious to the recipient thereof.
Conveniently, the active ingredient comprises from 0.05-99.9% by weight of the formulation.
In the form of a dosage unit, the compound may be administered one or more times a day at appropriate intervals, always depending, however, on the condition of the patient, and in accordance with the prescription made by the medical practitioner. Conveniently, a dosage unit of a formulation contain between 0.1 mg and 1000 mg, preferably between 1 mg and 100 mg, such as 5-50 mg of a compound of formula A. A suitable dosage of the compound of the invention will depend, inter alia, on the age and condition of the patient, the severity of the disease to be treated and other factors well known to the practising physician. The compound may be administered orally, parenterally or topically according to different dosing schedules, e.g. daily or with weekly intervals. In general a single dose will be in the range from 0.01 to 400 mg/kg body weight. The compound may be administered as a bolus (i.e. the entire daily dosis is administered at once) or in divided doses two or more times a day.
In the context of topical treatment it may be more appropriate to refer to a “usage unit”, which denotes a single dose which is capable of being administered to a patient, and which may be readily handled and packed, remaining as a physically and chemically stable unit dose comprising either the active material as such or a mixture of it with solid or liquid pharmaceutical diluents or carriers.
The term “usage unit” in connection with topical use means a unitary, i.e. a single dose capable of being administered topically to a patient in an application per square centimetre of the infected area of from 0.1 mg to 10 mg and preferably from 0.2 mg to 1 mg of the active ingredient in question.
It is also envisaged that in certain treatment regimes, administration with longer intervals, e.g. every other day, every week, or even with longer intervals may be beneficial.
If the treatment involves administration of another therapeutically active compound it is recommended to consult Goodman & Gilman's The Pharmacological Basis of Therapeutics, 9th Ed., J. G. Hardman and L. E. Limbird (Eds.), McGraw-Hill 1995, for useful dosages of said compounds.
The administration of a compound of the present invention with one or more other active compounds may be either concomitantly or sequentially.
The formulations include e.g. those in a form suitable for oral (including sustained or timed release), rectal, parenteral (including subcutaneous, intraperitoneal, intramuscular, intraarticular and intravenous), transdermal, ophthalmic, topical, dermal, nasal, buccal or intradermal administration. Topical administration of the claimed formulation is particularly suitable.
The formulations may conveniently be presented in dosage unit form and may be prepared by any of the methods well known in the art of pharmacy, e.g. as disclosed in Remington, The Science and Practice of Pharmacy, 20th ed., 2000. All methods include the step of bringing the active ingredient into association with the carrier, which constitutes one or more accessory ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active ingredient into association with a liquid carrier or a finely divided solid carrier or both, and then, if necessary, shaping the product into the desired formulation.
Formulations of the present invention suitable for oral administration may be in the form of discrete units as capsules, sachets, tablets or lozenges, each containing a predetermined amount of the active ingredient; in the form of a powder or granules; in the form of a solution or a suspension in an aqueous liquid or non-aqueous liquid, such as ethanol or glycerol; or in the form of an oil-in-water emulsion or a water-in-oil emulsion. Such oils may be edible oils, such as e.g. cottonseed oil, sesame oil, coconut oil or peanut oil. Suitable dispersing or suspending agents for aqueous suspensions include synthetic or natural gums such as tragacanth, alginate, acacia, dextran, sodium carboxymethylcellulose, gelatin, methylcellulose, hydroxypropylmethylcellulose, hydroxypropylcellulose, carbomers and polyvinylpyrrolidone. The active ingredients may also be administered in the form of a bolus, electuary or paste.
A tablet may be made by compressing or moulding the active ingredient optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing, in a suitable machine, the active ingredient(s) in a free-flowing form such as a powder or granules, optionally mixed by a binder, such as e.g. lactose, glucose, starch, gelatine, acacia gum, tragacanth gum, sodium alginate, carboxymethylcellulose, methylcellulose, hydroxypropylmethylcellulose, polyethylene glycol, waxes or the like; a lubricant such as e.g. sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride or the like; a disintegrating agent such as e.g. starch, methylcellulose, agar, bentonite, croscarmellose sodium, sodium starch glycollate, crospovidone or the like or a dispersing agent, such as polysorbate 80. Moulded tablets may be made by moulding, in a suitable machine, a mixture of the powdered active ingredient and suitable carrier moistened with an inert liquid diluent.
Formulations for rectal administration may be in the form of suppositories in which the compound of the present invention is admixed with low melting water soluble or insoluble solids such as cocoa butter, hydrogenated vegetable oils, polyethylene glycol or fatty acids esters of polyethylene glycols, while elixirs may be prepared using myristyl palmitate.
Formulations suitable for parenteral administration conveniently comprise a sterile oily or aqueous preparation of the active ingredients, which is preferably isotonic with the blood of the recipient, e.g. isotonic saline, isotonic glucose solution or buffer solution. The formulation may be conveniently sterilised by for instance filtration through a bacteria retaining filter, addition of sterilising agent to the formulation, irradiation of the formulation or heating of the formulation. Liposomal formulations as disclosed in e.g. Encyclopedia of Pharmaceutical Technology, vol. 9, 1994, are also suitable for parenteral administration.
Alternatively, the compounds of formula A may be presented as a sterile, solid preparation, e.g. a freeze-dried powder, which is readily dissolved in a sterile solvent immediately prior to use.
Transdermal formulations may be in the form of a plaster, patch, microneedles, liposomal or nanoparticulate delivery systems or other cutaneous formulations applied to the skin.
Formulations suitable for ophthalmic administration may be in the form of a sterile aqueous preparation of the active ingredients, which may be in microcrystalline form, for example, in the form of an aqueous microcrystalline suspension. Liposomal formulations or biodegradable polymer systems e.g. as disclosed in Encyclopedia of Pharmaceutical Technology, vol. 2, 1989, may also be used to present the active ingredient for ophthalmic administration.
Formulations suitable for topical, such as dermal, intradermal or ophthalmic administration include liquid or semi-liquid preparations such as liniments, lotions, gels, sprays, micro or nano-emulsions, oil-in-water or water-in-oil emulsions such as creams, ointments, pastes, applicants, foams, filmforming systems or microneedles; or solutions or suspensions such as drops. Compositions for ophthalmic treatment may preferably additionally contain a cyclodextrin.
For topical administration, the compound of formula A may typically be present in an amount of from 0.01 to 20% by weight of the composition, such as 0.1% to about 10%, but may also be present in an amount of up to about 50% of the composition.
Formulations suitable for nasal or buccal administration include powder, self-propelling and spray formulations, such as aerosols and atomisers. Such formulations are disclosed in greater detail in e.g. Modern Pharmaceutics, 2nd ed., G. S. Banker and C. T. Rhodes (Eds.), page 427-432, Marcel Dekker, New York; Modern Pharmaceutics, 3th ed., G. S. Banker and C. T. Rhodes (Eds.), page 618-619 and 718-721, Marcel Dekker, New York and_Encyclopedia of Pharmaceutical Technology, vol. 10, J. Swarbrick and J. C. Boylan (Eds), page 191-221, Marcel Dekker, New York.
In addition to the aforementioned ingredients, the formulations of a compound of formula A may include one or more additional ingredients such as diluents, buffers, flavouring agents, colourant, surface active agents, thickeners, preservatives, e.g. methyl hydroxybenzoate (including anti-oxidants), emulsifying agents and the like.
When the active ingredient is administered in the form of salts with pharmaceutically acceptable non-toxic acids, preferred salts are for instance easily water-soluble or slightly soluble in water, in order to obtain a particular and appropriate rate of absorption.
The pharmaceutical composition may additionally comprise one or more other active components conventionally used in the treatment of dermal disease or conditions, e.g. selected from the group consisting of glucocorticoids, vitamin D and vitamin D analogues, antihistamines, phosphodiesterase 4 (PDE4) inhibitors, JAK inhibitors, methylxanthines, β-adrenergic agents, COX-2 inhibitors, salicylates, indomethacin, flufenamate, naproxen, timegadine, retinoids, zinc salts, salicylazosulfapyridine and calcineurin inhibitors.
The term “compound of formula A” as used herein is intended to include compounds of formula A(i), formula A(ii) and formula A(iii).
The invention is further described in the appended examples which are not in any way intended to limit the scope of the invention as claimed.
The compounds of the present invention can be prepared in a number of ways well known to those skilled in the art of synthesis. The compounds of formula A may for example be prepared using the reactions and techniques outlined below together with methods known in the art of synthetic organic chemistry, or variations thereof as appreciated by those skilled in the art. Preferred methods include, but are not limited to, those described below. The reactions are carried out in solvents appropriate to the reagents and materials employed and suitable for the transformations being effected. Also, in the synthetic methods described below, it is to be understood that all proposed reaction conditions, including choice of solvent, reaction atmosphere, reaction temperature, duration of experiment and work-up procedures, are chosen to be conditions of standard for that reaction, which should be readily recognized by one skilled in the art of organic synthesis. Not all compounds falling into a given class may be compatible with some of the reaction conditions required in some of the methods described. Such restrictions to the substituents which are compatible with the reaction conditions will be readily apparent to one skilled in the art and alternative methods can be used.
Starting materials of general formula II are prepared according to standard procedures known to a chemist skilled in the art of organic synthesis. An aldehyde of formula I is treated with (S)-2-methylpropane-2-sulfinamide and Ti(O-iPr)4 in a suitable solvent (e.g. THF) under suitable conditions (e.g. 0° C. to RT) for a suitable period of time (e.g. 5-10 h) to form a compound of general formula II.
A compound of general formula III can be prepared as described in Scheme 2. A sulfinamide derivative of formula II is treated with methyl magnesium bromide in a suitable solvent (e.g. DCM) under suitable conditions (e.g. −50° C. to RT) for a suitable period of time (e.g. 2-5 h) to form compound III.
A compound of general formula IV can be prepared as described in Scheme 3. A sulfinamide derivative of general formula III is treated with a suitable base (e.g. LiHMDS) in a suitable solvent (e.g. THF), under suitable conditions (e.g. −70° C. to RT) for a suitable period of time (e.g. 1 h), followed by addition of iodomethane, for a suitable period of time (e.g. 12 h) to form a compound of general formula IV.
A compound of general formula V can be prepared as described in Scheme 4. A compound of general formula IV is treated with hydrogen chloride in a suitable solvent (e.g. MeOH), for a suitable period of time (e.g. 1 h) at a suitable temperature (e.g. RT) to form a compound of general formula V.
A compound of general formula VIII can be prepared as described in Scheme 5. A diamine derivative of general formula VI is treated with a suitable acylating agent (e.g. benzyl chloroformate) in a suitable solvent (e.g. DCM), under suitable conditions (e.g. 0° C. to RT) for a suitable period of time (e.g. 3 h) to form a compound of general formula VII, which in turn is treated with pentynoic acid in the presence of suitable coupling agents (e.g. EDCI, HOBT), in a suitable solvent (e.g. DCM), under suitable conditions (e.g. 0° C. to RT) in the presence of a suitable base (e.g. TEA) for a suitable period of time (e.g. 12 h) to form a compound of general formula VIII.
A compound of general formula IX can be prepared as described in Scheme 6. A compound of general formula VIII is treated with 2-Iodotoluene in a suitable solvent (e.g. DMF), in the presence of a suitable base (e.g. TEA), under suitable coupling conditions (e.g. catalytic amounts of copper iodide and a palladium catalyst such as PdCl2(PPh3)2), for a suitable period of time (e.g. 3 h) at a suitable temperature (e.g. RT) to form a compound of general formula IX.
A compound of general formula X can be prepared as described in Scheme 7. A compound of general formula IX is treated with a suitable oxidizing agent (e.g. PIFA) in a suitable solvent (e.g. TFEA) for a suitable period of time (e.g. 3 h) at a suitable temperature (e.g. 0° C. to RT) to form a compound of general formula X.
A compound of general formula XI can be prepared as described in Scheme 8. A compound of general formula X is treated under suitable hydrogenation conditions (e.g. 5 Atm H2) in a suitable acidic media (e.g. HCl/MeOH) for a suitable period of time (e.g. 24 h) and in the presence of a suitable catalyst (e.g. Pd/C 5%) to afford a compound of general formula XI.
An enantiomerically pure compound of formula XIII can be prepared as described in Scheme 9. A compound of general formula XII is treated with a suitable base (e.g. LiHMDS) in a suitable solvent (e.g. THF) for a suitable period of time (e.g. 0.5-1 h), followed by addition of a suitable reagent (e.g. 2-bromoacetonitrile), for a suitable period (e.g. 1-2 h) to afford a compound of general formula XIII.
An enantiomerically pure compound of general formula XIV can be prepared as described in Scheme 10. A compound of general formula XIII is treated under suitable hydrogenation conditions (e.g. 4 Atm H2) in a suitable basic media (e.g. NH4OH/MeOH) for a suitable period of time (e.g. 24 h) and in the presence of a suitable catalyst (e.g. Raney Ni) to afford a compound of general formula XIV.
An enantiomerically pure compound of general formula XV can be prepared as described in Scheme 11. A compound of general formula XIV is treated with a suitable reagent (e.g. Boc anhydride) in the presence of a suitable base (e.g. TEA) in a suitable solvent (e.g. DCM) at a suitable temperature (e.g. RT) for a suitable period of time (e.g. 12 h) to give a compound of formula XV.
An enantiomerically pure compound of general formula XVI can be prepared as described in Scheme 12. A compound of general formula XV is treated with a suitable reagent (e.g. Lithium reagent) in a suitable solvent (e.g. THF) at a suitable temperature (e.g. −70° C.) for a suitable period of time (e.g. 5-10 min) to give a compound of formula XVI.
An enantiomerically pure compound of general formula XVII can be prepared as described in Scheme 13. A compound of general formula XVI is treated with a suitable reduction reagent (e.g. LiAl(OtBu3)H) in a suitable solvent (e.g. THF) at a suitable temperature (e.g. RT) for a suitable period of time (e.g. 1.5 h) to give a compound of formula XVII.
An enantiomerically pure compound of general formula XVIII can be prepared as described in Scheme 14. A compound of general formula XVII is treated with a suitable reagent (e.g. methanesulfonyl chloride) in the presence of a suitable base (e.g. TEA, DMAP) in a suitable solvent (e.g. DCM) at a suitable temperature (e.g. RT) for a suitable period of time (e.g. 20 h) to give a compound of formula XVIII.
An enantiomerically pure compound of general formula XIX can be prepared as described in Scheme 15. A compound of general formula XVIII is treated with a suitable acid (e.g. TFA) in a suitable solvent (e.g. DCM) to afford a compound of general formula XIX.
A compound of general formula XX can be prepared as described in Scheme 16. A compound of general formula XI or XIX is treated with a suitable acylating agent (e.g. triphosgene) in a suitable solvent (e.g. EtOAc) in the presence of a compound of general formula V and of a suitable base (e.g. TEA), at a suitable temperature (e.g. 0° C. to RT) for a suitable period of time (e.g. 3 h) to afford a compound of general formula XX.
A compound of general formula XXII can be prepared as described in Scheme 17. A compound of general formula XXI or XVIII is treated with a suitable acylating or alkylating agent (methyl chloroformate (possibly followed by MeI), allyl bromide) in the presence of a suitable base (e.g. LiHMDS) in a suitable solvent (e.g. THF) at a suitable temperature (e.g. −78° C. to 0° C.) for a suitable period of time (e.g. 3 h). The resulting compound (XXII, R=Boc) can in turn be treated with a suitable acid (e.g. TFA) in a suitable solvent (e.g. DCM) to afford a compound of general formula XXII (R═H).
A compound of general formula XXIII can be prepared as described in Scheme 18. A compound of general formula XXII is treated as described for compound of general formula XI to give the urea derivative of general formula XX. Further elaboration of V, V′ residues, using standard methods well described in the literature and well known to those skilled in the art (e.g. cleavage of an allylic double bond by ozonolysis to form the corresponding aldehyde and subsequent reduction of the aldehyde to form the corresponding alcohol, derivatization of the alcohol to form a mesylate) can lead to further derivatives also represented in the general formula XXIII.
Proton Magnetic Resonance (NMR) spectra were recorded on a Bruker instrument at 400 MHz spectrometers at 25° C. Chemical shifts are reported in ppm (δ) using the residual solvent line as internal standard. Peak multiplicities are expressed as follow: s, singlet; d, doublet; dd doublet of doublets, t, triplet; dt doublet of triplet; q, quartet; quin, quintet; m, multiplet; br s, broad singlet.
Total ion current (TIC) and DAD UV chromatographic traces together with MS and UV spectra associated with the peaks were taken also on a SHIMADZU LCMS2020 system equipped with a LCMS2020 mass spectrometer operating in positive and/or negative electrospray ionisation mode. [LC/MS/ESI (+/−): analyses performed using a Chromolith SpeedROD C18 column (50×4.6 mm, 2 μm particle size), column temperature 35° C., mobile phase: A=CH3CN/H2O/FA=10/90/0.05 and B=CH3CN/H2O/FA=90/10/0.05, Flow rate: 3.0 mL/min, Gradient: t=0.8 min 20% B, t=3.5 min 95% B, t=4.3 min 95% B, t=4.4 min 20% B.
Achiral purification were carried out using a SHIMADZU 6A system operated under follow chromatographic conditions, mobile phase: A=0.1% FA in H2O and B=MeCN, flow rate 12 ml/min and column Shimapack-ODS(H) KIT (250*21.2 mm, 10 um) @room T.
For the chiral separation and the chiral quality control two different techniques were used: 1) Supercritical Fluid Choromatography (SFC): analytical chromatography was performed on a Berger SFC Analytix, while for the preparative SFC, a Jasco preparative SFC system was used 2) High Performance Liquid Chromatography (HPLC): chiral Preparative HPLC was performed using a Waters 600 HPLC system and Agilent series 1100 instrument, while for analytical chromatography a SHIMADZU LC-2010A HPLC was used.
AcOH: Acetic acid
Boc: tert-butyloxycarbonyl
EtOAc: ethyl acetate
CH: cyclohexane
Cy: cyclohexane
DCM: dichloromethane
DIBAL-H: Diisobutylaluminium hydride
DMSO: dimethylsulfoxide
DMPU: 1,3-Dimethyl-3,4,5,6-tetrahydro-2(1H)-pyrimidinone
EDCI.HCl: N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride
EtOH: ethanol
HOBT.H2O: hydroxybenzotriazol monohydrate
LHMDS: lithium bis(trimethylsilyl)amide
MeCN: acetonitrile
MeOH: methanol
Pd(dppf)Cl2: [1,1′-Bis(diphenylphosphino)ferrocene]dichloropalladium(II)
iPr: isopropyl
PIFA ((bis(trifluoroacetoxy)iodo)benzene
RT: room temperature
ee: enantiomeric eccess
Rt: retention time
TEA: triethylamine
TFA: trifluoroacetic acid
TFEA: 2,2,2-trifluoroethanol
THF: tetrahydrofuran
The bicyclic corestructure of the compounds of the present invention is numbered as follows:
To a solution of benzyl 2-pent-4-ynamidoethylcarbamate (7 g, 25.5 mmol) in DMF (100 mL), was added 1-iodo-2,4-dimethylbenzene (6.7 g, 29 mmol), TEA (4.2 mL, 30.4 mmol), CuI (420 mg, 2.2 mmol) and PdCl2(PPh3)2 (780 mg, 1.1 mmol) under N2 atmosphere. The resulting mixture was stirred at 25° C. for 12 h and concentrated. The residue was dissolved in DCM (100 mL) and washed with 1 M HCl solution and brine. The organic layer was dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/3 to 1/10) to give the title compound (6.1 g, 63%); m/z (ES+): 379 [M+H]+.
To a solution of benzyl 2-(5-(2,4-dimethylphenyl)pent-4-ynamido)ethylcarbamate (6.5 g, 16.1 mmol) in TFEA (250 mL) was cooled and stirred at 0° C., then a solution of PIFA (12.3 g, 28.7 mmol) in TFEA (150 mL) was added dropwise. The reaction was stirring at 0° C. for 2 h and then quenched by addition of sat. Na2CO3 solution. The resulting mixture was extracted with DCM (3×100 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/10) to give the title compound (5 g, 79%); m/z (ES+): 395 [M+H]+
To a solution of benzyl 2-(2-(2,4-dimethylbenzoyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (7 g, 17.8 mmol) in 80 ML of MeOH was added 1 M HCl (35 mL) and Pd/C 5% (1 g). The reaction was stirred under H2 (5 atm) atmosphere for 12 h. The reaction mixture was filtered through a celite pad and concentrated in high vacuum. The residue was dissolved in MeOH (30 ml) and NaBH3(CN) (1.3 g, 20.5 mmol) was added. The reaction was stirred for 5 h at room temperature and filtered. The filtrate was concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/2) to give the title compound (2.3 g, 53%); m/z (ES+): 245 [M+H]+
To a solution of 1-(2,4-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (mixture of trans, 1.5 g, 6.1 mmol) was added (Boc)2O (2.65 g, 12.2 mmol) at 0° C., the reaction mixture was stirred overnight at 25° C., then N1,N1-dimethylethane-1,2-diamine (5 ml) was added and the mixture was stirred at 25° C. for 8 h. Saturated solution of NH4Cl was added, followed by EtOAc (2×20 mL). The combined organic layers were dried over sodium sulphate, filtered and concentrated in vacuo, to give the title compound (2 g, 95%) which was used in the next step without further purifications; m/z (ES+): 345 [M+H]+.
To a solution of tert-butyl 1-(2,4-dimethylphenyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (mixture of trans, 2 g, 5.8 mmol) in anhydrous THF (60 mL) under N2 atmosphere, was added LiHMDS (1 M in THF, 6.05 mL) at −78° C. The reaction was stirred for 15 min, followed by addition of DMPU (1.8 mL, 15 mmol) and allylbromide (0.52 mL, 6 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C. and the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (2 g, yield: 81%); m/z (ES+): 425 [M+H]+.
To a solution of tert-butyl 7,7-diallyl-1-(2,4-dimethylphenyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (mixture of trans, 2 g, 4.7 mmol) in DCM (40 mL) at 0° C. was added TFA (8 mL). The reaction was stirred at room temperature for 3 h. DCM was removed under reduced pressure, the residue was adjusted with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (1.2 g, yield: 79%); m/z (ES+): 325 [M+H]+.
To a solution of triphosgene (205 mg, 0.70 mmol) in EtOAc (10 mL) at 0° C. was added solution of 7,7-diallyl-1-(2,4-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (mixture of trans, 500 mg, 1.54 mmol), DMAP (40 mg, 0.32 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (10 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (543 mg, 2 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×100 mL); the combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title intermediate 7 (200 mg, yield: 20%) and 8 (180 mg, yield: 18%); m/z (ES+): 622 [M+H]+.
To a solution of triphosgene (205 mg, 0.70 mmol) in EtOAc (10 mL) at 0° C. was added solution of 7,7-diallyl-1-(2,4-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (mixture of trans, 500 mg, 1.54 mmol), DMAP (40 mg, 0.32 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (10 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (3,5-bis(trifluoromethyl)phenyl)-N-methylmethanamine (514 mg, 2 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×100 mL); the combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (160 mg, yield: 16%); m/z (ES+): 608 [M+H]+.
Intermediate 10 was synthesized using the same procedure for intermediate 6. m/z (ES+): 329 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (1 mL) at 0° C. was added solution of intermediate 10 (mix of trans, 100 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 2 h, followed by addition of (R)-1-(3-chloro-5-(trifluoromethyl)phenyl)-N-methylethanamine (100 mg, 0.44 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The reaction was stirred at 50° C. for 48 h and quenched with water. The resulting mixture was extracted with EtOAc (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound (66 mg, yield: 33%); m/z (ES+): 592 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (1 mL) at 0° C. was added solution of intermediate 10 (mix of trans, 100 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 2 h, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl)phenyl)ethanamine (100 mg, 0.44 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The reaction was stirred at 50° C. for 48 h and quenched with water. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound (100 mg, yield: 51%); m/z (ES+): 572 [M+H]+.
To a solution of triphosgene (149 mg, 0.5 mmol) in EtOAc (5 mL) at 0° C. was added solution of intermediate 10 (mix of trans, 328 mg, 1 mmol), DMAP (12 mg, 0.1 mmol) and TEA (151 mg, 1.5 mmol) in EtOAc (5 mL). The mixture was stirred for 2 h, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (407 mg, 1.5 mmol) and TEA (151 mg, 1.5 mmol) in EtOAc (5 mL). The reaction was stirred at 50° C. for 48 h and quenched with water. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound (200 mg, yield: 32%); m/z (ES+): 626 [M+H]+.
Intermediate 14 was prepared using the procedure for intermediate 3. m/z (ES+): 261 [M+H]+.
Intermediate 15 was prepared using the procedure for intermediate 3 to prepare. m/z (ES+): 341 [M+H]+.
Intermediate 16 was prepared using the procedure for intermediate 6 to prepare. m/z (ES+): 341 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 15 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (139 mg, 0.6 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL). The organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (65 mg, yield: 31%); m/z (ES+): 598 [M+H]+.
To a suspension of NaH (60%) (27.9 g, 0.7 mol) in THF (800 mL), was dropwise added (S)-methyl 5-oxopyrrolidine-2-carboxylate (100.0 g, 0.7 mol) in THF (200 mL) at 0° C. The reaction was stirred at this temperature for 30 min followed by addition of bromoacetonitrile (48.4 ml, 0.7 mol) at 0° C. The reaction mixture was allowed to warm to 15° C. and stirred for 1 h, and then carefully poured into a mixture of ice-water (200 mL) and H2SO4 (6 mL). The resulting mixture was extracted with EtOAc (300 ml), washed with brine (2×100 mL). The organic layer was dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=4/1 to 1/1) to give the title compound (65 g, yield: 51%, ee=96.9%); m/z (ES+): 183 [M+H]+.
To a solution of Intermediate 1 (20 g, 0.11 mol) in MeOH (300 mL) under N2 atmosphere, was added Raney Ni (5.0 g) and followed by NH4OH (0.2 mL). The reaction mixture was stirred under H2 atmosphere (4 atm) for 24 h at room temperature. Raney Ni was filtered through a celite pad, and the filtrate was concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=70/1 to 10/1) to give the title compound (8.3 g, yield: 52%, ee=94.2%); m/z (ES+): 155 [M+H]+.
To a solution of Intermediate 2 (14.0 g, 90.3 mmol), Boc2O (39.4 g, 182.0 mmol) and DMAP (550 mg, 4.5 mmol) in THF (250 mL) was dropwise added Et3N (26.2 mL, 182.0 mmol). The reaction was stirred at room temperature for 3 h and quenched by addition of H2O. The resulting mixture was extracted with EtOAc (2×100 mL). The combined organic layers were dried over anhydrous Na2SO4, and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/3) to give the title compound (17.0 g, yield: 74%, ee=92.9%); m/z (ES+): 255 [M+H]+.
To a solution of 1-bromo-4-fluoro-2-methyl-benzene (2.34 g, 12.39 mmol) in THF (40 mL) at −70° C. was dropwise added n-BuLi (1.6 M in hexane, 7.52 mL, 12 mmol) over 5 min. After addition, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1, 6-dioxohexahydro pyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (4.2 g, yield: 60%); m/z (ES+): 365 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(4-fluoro-2-methylbenzoyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2.3 g, 6.3 mmol) in THF (50 mL), was portionwise added LiAl(OtBu3)H (3.56 g, 14 mmol) at −20° C. The mixture was stirred for 1.5 h at 20° C. and then quenched with water. The resulting mixture was filtered through a celite pad and the filtration was extracted with EtOAc (2×50 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.1 g, yield: 90%); m/z (ES+): 379[M+H]+.
To a solution of tert-butyl 2-((S)-2-((R)-(4-fluoro-2-methylphenyl)(hydroxy)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (3.6 g, 9.8 mmol) in DCM (20 mL) was added Et3N (3.54 g, 35 mmol) and DMAP (85.6 mg, 0.7 mmol). The resulting solution was cooled to 0° C., and then MsCl (1.18 g, 10.3 mmol) was dropwise added into under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (700 mg, yield: 20%); m/z (ES+): 349 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(4-fluoro-2-methylphenyl)-6-oxohexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (700 mg, 2.0 mmol) in anhydrous THF (100 mL) under N2 atmosphere at −78° C. was added LiHMDS (1 M in THF, 3.4 mL). The reaction was stirred for 15 min at this temperature, followed by addition of DMPU (895 mg, 7 mmol) and allylbromide (316.5 mg, 2.62 mmol). The reaction mixture was stirred at −78° C. for another 30 min. allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C., the mixture was then extracted with EtOAc (2×50 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (650 mg, yield: 81%); m/z (ES+): 429 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(4-fluoro-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (220 mg, 0.59 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The resulting mixture was extracted with EtOAc (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was directly used in the next step without further purification (180 mg, yield: 95%); m/z (ES+): 249 [M+H]+.
To a solution of 7,7-Diallyl-1-(4-fluoro-2-methyl-phenyl)-6-oxo-hexahydro-pyrrolo[1,2-a]pyrazine-2-carboxylic acid tert-butyl ester (850 mg, 1.98 mmol) in MeOH (20 mL) was added HCl/MeOH (7 M, 5 mL) at 0° C. The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The resulting mixture was extracted with EtOAc (2×30 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was directly used in the next step without further purification (650 mg, yield: 95%); m/z (ES+): 329 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added a solution of (1S,8aS)-7,7-diallyl-1-(4-fluoro-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (164 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3-Ethyl-5-trifluoromethyl-phenyl)-ethyl]-methyl-amine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (120 mg, yield: 39%); m/z (ES+): 586 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added a solution of (1S,8aS)-7,7-diallyl-1-(4-fluoro-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (164 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.7 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3-Isopropyl-5-trifluoromethyl-phenyl)-ethyl]-methyl-amine (163.2 mg, 0.6 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL) and washed with brine. The combined organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (110 mg, yield: 37%); m/z (ES+): 600 [M+H]+.
To a solution of 1-bromo-4-methoxy-2-methylbenzene (2.0 g, 10 mmol) in THF (20 mL) at −70° C. was dropwise added n-BuLi (1.6 M in hexane, 7.52 mL, 12 mmol) over 5 min. The reaction was stirred at this temperature for 30 min. The resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (2.5 g, 10 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL). The organic layers were washed with brine, dried over Na2SO4, filtered and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.3 g, yield: 60%); m/z (ES+): 377 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(4-methoxy-2-methylbenzoyl)-5-oxopyrrolidin-1-yl) ethylcarbamate (2.3 g, 6.1 mmol) in THF (50 mL), was portionwise added LiAl(OtBu3)H (3.56 g, 14 mmol) at −20° C. The mixture was stirred for 1.5 h at 20° C. and then quenched with water. The resulting mixture was filtered through a celite pad and the filtration was extracted with EtOAc (2×50 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.3 g, yield: 99.7%); m/z (ES+): 379[M+H]+.
To a solution of tert-butyl 2-((S)-2-((R)-hydroxy(4-methoxy-2-methylphenyl)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2.3 g, 6.1 mmol) in DCM (20 mL) was added Et3N (3.54 g, 35 mmol), DMAP (85.6 mg, 0.7 mmol). The resulting solution was cooled to 0° C., and then MsCl (1.18 g, 10.3 mmol) was added into the above solution dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution and extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (1.5 mg, yield: 67%); m/z (ES+): 361 [M+H]+.
To a solution of (1R,8aS)-tert-butyl 1-(4-methoxy-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (0.5 g, 1.4 mmol) in DCM (10 mL) was added TFA (2.5 mL). The reaction was stirred at room temperature for 3 h and then basified with 1 M aqueous NaOH solution to pH=8. The resulting mixture was extracted with DCM (2×30 mL). The combined organic layers were dried over anhydrous Na2SO4, and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound (0.4 g, yield: 100%); m/z (ES+): 261 [M+H]+.
To a solution of (1R,8aS)-tert-butyl 1-(4-methoxy-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (1 g, 2.7 mmol) in anhydrous THF (15 mL) under N2 atmosphere at −78° C. was added LiHMDS (1 M in THF, 13.5 mL). The reaction was stirred for 15 min, followed by addition of DMPU (1.3 g, 10.8 mmol) and allylbromide (1.3 g, 10.8 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C. The mixture was stirred at this temperature for another 2 h. After quenched with water at −78° C., the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (1.1 g, yield: 92.6%); m/z (ES+): 441 [M+H]+.
To a solution of (1R,8aS)-tert-butyl 7,7-diallyl-1-(4-methoxy-2-methylphenyl)-6-oxohexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (1.1 g, 2.5 mmol) in MeOH (20 mL) was added HCl/MeOH (7 M, 15 mL) at 0° C. The reaction was stirred at room temperature for 3 h and then basified with 1 M aqueous NaOH solution to pH=8. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was directly used in the next step without further purification (811 mg, yield: 95%); m/z (ES+): 341 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(4-methoxy-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (70 mg, yield: 31%); m/z (ES+): 638 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(4-methoxy-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl)phenyl)ethanamine (139 mg, 0.6 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL). The organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (82 mg, yield: 40%); m/z (ES+): 584 [M+H]+.
To a solution of 1-(benzyloxy)-4-bromo-2-methylbenzene (3.6 g, 12.9 mmol) in THF (40 mL) at −70° C. was added n-BuLi (1.6 M, 7.7 ml, 12.3 mmol). The reaction was stirred at the same temperature for 30 min, and added to (S)-tert-butyl 1,6-dioxohexahydro pyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (3.0 g, 11.7 mmol) in 40 ml THF. After stirred for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10/1 to 1/1) to give the title compound (2.0 g, yield: 38%); m/z (ES+): 453 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(4-(benzyloxy)-2-methylbenzoyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2 g, 4.4 mmol) in THF (50 mL), was portionwise added LiAl(OtBu3)H (1.68 g, 6.6 mmol) at −20° C. The mixture was stirred for 1.5 h at 20° C. and then quenched with water. The resulting mixture was filtered through a celite pad and the filtrate was extracted with EtOAc (2×50 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (1.85 g, yield: 92.4%); m/z (ES+): 455[M+H]+
To a solution of tert-butyl 2-((S)-2-((R)-(4-(benzyloxy)-2-methylphenyl)(hydroxy) methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (1.85 g, 4.0 mmol) in DCM (20 mL) was added Et3N (2.02 g, 20 mmol), DMAP (85.6 mg, 0.7 mmol). The resulting solution was cooled to 0° C., and then MsCl (860 mg, 6.0 mmol) was dropwise added into the above solution under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (1.2 g, yield: 69%); m/z (ES+): 437 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(4-(benzyloxy)-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (200 mg, 0.46 mmol) in MeOH (5 mL) was added HCl/MeOH (7M, 2 mL) at 0° C. The reaction was stirred at room temperature for 3 h and then basified with 1 M aqueous NaOH solution to pH=8. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was directly used in the next step without further purification (100 mg, yield: 65%); m/z (ES+): 337 [M+H]+.
To a solution of triphosgene (73 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-1-(4-(benzyloxy)-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (162 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.7 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (163.2 mg, 0.6 mmol) and (151.7 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in vacuum. The residue was purified by Prep-HPLC to give the title compound (120 mg, yield: 39%); m/z (ES+): 594 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(4-(benzyloxy)-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (600 mg, 1.37 mmol) in anhydrous THF (30 mL) under N2 atmosphere at −78° C. was added LiHMDS (1 M in THF, 5.49 mL). The reaction was stirred for 15 min, followed by addition of DMPU (870 mg, 6.85 mmol) and allylbromide (400 mg, 3.42 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C. The reaction was stirred at this temperature for another 2 h. After quenched with water at −78° C. and the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (600 mg, yield: 85%); m/z (ES+): 425 [M+H]+.
To a solution of (1R,8aS)-tert-butyl7,7-diallyl-1-(4-(benzyloxy)-2-methylphenyl)-6-oxohexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (600 mg, 1.16 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was directly used in the next step without further purification (400 mg, yield: 83%); m/z (ES+): 417 [M+H]+.
To a solution of triphosgene (53 mg, 0.18 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(4-(benzyloxy)-2-methylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (150 mg, 0.36 mmol), DMAP (4 mg, 0.05 mmol) and TEA (101 mg, 1 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (147 mg, 0.54 mmol) in EtOAc (2 mL) and TEA (101 mg, 1 mmol). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (110 mg, yield: 42%); m/z (ES+): 714 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of (1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-7,7-diallyl-1-(4-(benzyloxy)-2-methylphenyl)-N-methyl-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide (110 mg, 0.14 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3, followed by addition of NaBH4 (106 mg, 2.8 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with saturated NH4Cl solution, the resulting mixture was extracted with DCM. The organic layer was washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (50 mg, yield: 50%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.55 (s, 2H), 7.44 (d, J=6.8 Hz, 2H), 7.40 (t, J=7.3 Hz, 2H), 7.34 (dd, J=8.3, 5.6 Hz, 1H), 7.16 (d, J=8.5 Hz, 1H), 6.82 (d, J=2.5 Hz, 1H), 6.75 (dd, J=8.5, 2.6 Hz, 1H), 5.56 (q, J=6.8 Hz, 1H), 5.03 (s, 2H), 4.10 (d, J=12.7 Hz, 1H), 3.98 (d, J=9.8 Hz, 1H), 3.81 (dq, J=11.6, 6.4 Hz, 3H), 3.71 (dt, J=11.3, 5.5 Hz, 1H), 3.65-3.52 (m, 1H), 3.27 (d, J=11.3 Hz, 1H), 3.06 (ddt, J=20.7, 11.7, 6.0 Hz, 3H), 2.69 (s, 3H), 2.50 (s, 3H), 1.85 (ddd, J=15.9, 11.9, 5.7 Hz, 4H), 1.78-1.65 (m, 3H), 1.44-1.37 (m, 3H); m/z (ES+): 722 [M+H]+.
To a solution of triphosgene (53.4 mg, 0.18 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(4-(benzyloxy)-2-methylphenyl) hexahydropyrrolo [1,2-a]pyrazin-6(7H)-one (150 mg, 0.36 mmol), DMAP (4.6 mg, 0.036 mmol) and TEA (109.2 mg, 1.08 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl) phenyl)-N-methylethanamine (100 mg, 0.432 mmol) and TEA (109.2 mg, 1.08 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with saturated NH4Cl (aq). The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (100 mg, yield: 41%); m/z (ES+): 674 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 46 (100 mg, 0.15 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3, followed by addition of NaBH4 (57 mg, 1.5 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with saturated NH4Cl solution, the resulting mixture was extracted with DCM. The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (50 mg, yield: 50%); m/z (ES+): 682 [M+H]+.
To a solution of 2-chloro-6-(trifluoromethyl)pyridine (25 g, 138 mmol) in THF (200 mL) was added Me3Al (1.0 M in toluene, 150 mL, 150 mmol) dropwise over 5 min by syringe at 0° C. After addition, the reaction was stirred at room temperature for 12 h. Then the reaction mixture was quenched with saturated NH4Cl solution and extracted with toluene (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated. The residue was used for next step directly; m/z (ES+): 162 [M+H]+.
To a solution of 2-methyl-6-(trifluoromethyl)pyridine (22.2 g, 138 mmol) in toluene (200 mL) was added mCPBA (23 g, 150 mmol). The reaction mixture was refluxed for 24 h under N2 atmosphere and quenched with saturated NH4Cl solution. The resulting mixture was extracted with EtOAc (2×150 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (5.5 g, yield: 22%); m/z (ES+): 178 [M+H]
To a solution of 2-methyl-6-(trifluoromethyl)pyridine 1-oxide (6 g, 33.7 mmol) in H2SO4 (20 mL), was added nitric acid fuming (23 g, 36 mmol). The resulting solution was refluxed for 1 h under N2 atmosphere. The reaction mixture was quenched with ice-water and extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (6 g, yield: 80%); m/z (ES+): 222 [M+H]
A solution of 2-methyl-4-nitro-6-(trifluoromethyl)pyridine 1-oxide (6 g, 27 mmol) in CH3COBr (20 mL) was stirred for 8 h and poured into ice-water. The resulting mixture was extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (3.8 g, yield: 55%); m/z (ES+): 257 [M+H]
To a solution of 4-bromo-2-methyl-6-(trifluoromethyl)pyridine 1-oxide (2.3 g, 8.9 mmol) in THF (10 mL), was added Pd(PPh3)4 (115 mg, 1 mmol) and ZnEt2 (1.0 M in toluene, 15 mmol). The resulting solution was stirred for 18 h at room temperature under N2 atmosphere and quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (1 g, yield: 54%); m/z (ES+): 206[M+H]+.
A solution of 4-ethyl-2-methyl-6-(trifluoromethyl)pyridine 1-oxide (1 g, 4.88 mmol) in Ac2O (10 mL) was stirred at 110° C. for 8 h. The resulting solution was poured into ice-water and extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (700 mg, yield: 57%); m/z (ES+): 248 [M+H]
To a solution of (4-ethyl-6-(trifluoromethyl)pyridin-2-yl)methyl acetate (950 mg, 3.83 mmol) in MeOH (20 mL) was added 10% NaOH aq (10 mL). The reaction mixture was stirred for 4 h. The resulting solution was poured into ice-water and extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (800 mg, yield: 100%); m/z (ES+): 206 [M+H]
To a solution of (4-ethyl-6-(trifluoromethyl)pyridin-2-yl)methanol (780 g, 3.78 mmol) in DCM (10 mL) at 0° C. was added Dess-Martin reagent (1.1 g, 4.5 mmol). The reaction was stirred for 20 min. The resulting solution was poured into ice-water and extracted with DCM (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 2/1) to give the title compound (640 mg, yield: 83%); m/z (ES+): 204[M+H]+.
To a solution of 4-ethyl-6-(trifluoromethyl)picolinaldehyde (640 mg, 3.14 mmol) in THF (10 mL) was added (S)-2-methylpropane-2-sulfinamide (480 mg, 4 mmol) and Ti(O-iPr)4 (1.3 g, 5 mmol) at 0° C. After stirring for 6 h at room temperature, the reaction mixture was poured into a suspension of kieselguhr in water (20 mL) with vigorous stirring. The precipitate was filtered off and the filtrate was extracted with EtOAc (2×30 mL). The combined organic layers were washed with brine (10 mL), dried by anhydrous MgSO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10/1 to 1/1) to give the title compound (600 mg, yield: 62%); m/z (ES+): 307 [M+H]+.
To a solution of (S)—N-((4-ethyl-6-(trifluoromethyl)pyridin-2-yl)methylene)-2-methylpropane-2-sulfinamide (600 mg, 1.94 mmol) in DCM (10 mL) at −40° C. was dropwise added methyl magnesium bromide (2.1 mL, 1M in THF, 2.1 mmol). After addition, the reaction was warmed to room temperature and stirred for 2 h. The reaction mixture was then poured into saturated aqueous NH4Cl solution and extracted with DCM (2×10 mL). The combined organic layers were washed with brine (2×10 mL), dried over anhydrous MgSO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10/1 to 1/1) to give the title compound (550 mg, yield: 88%); m/z (ES+): 323 [M+H]+.
To a solution of (S)—N—((R)-1-(4-ethyl-6-(trifluoromethyl)pyridin-2-yl)ethyl)-2-methylpropane-2-sulfinamide (550 mg, 1.7 mmol) in THF (10 mL) under N2 atmosphere was added LiHMDS (3.2 mL, 1 M in THF, 3.2 mmol) at −60° C. The reaction was stirred for 15 min followed by addition of MeI (450 mg, 3.2 mmol). The reaction mixture was then stirred at room temperature for another 1 h and quenched with water at 0° C. The resulting mixture was then extracted with EtOAc (2×10 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10/1 to 1/1) to give the title compound (500 mg, yield: 87%); m/z (ES+): 337 [M+H]+.
A solution of (S)—N—((R)-1-(4-ethyl-6-(trifluoromethyl)pyridin-2-yl)ethyl)-N,2-dimethylpropane-2-sulfinamide (500 mg, 1.48 mmol) in HCl/MeOH (10 mL) was stirred for 15 min under N2 atmosphere. The reaction was basified with 1 M NaOH to pH=10-11 and then extracted with EtOAc (2×20 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated to give the title compound (380 mg, yield: 110%); m/z (ES+): 233 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-o-tolylhexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (100 mg, 0.32 mmol), DMAP (4 mg, 0.032 mmol) and TEA (48 mg, 0.48 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of N-methyl-1-(4-methyl-6-(trifluoromethyl)pyridin-2-yl)methanamine (97 mg, 0.96 mmol) and TEA (48 mg, 0.48 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl(aq). The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (90 mg, yield: 52.1%); m/z (ES+): 541 [M+H]+.
Intermediate 61 was prepared using the procedure for intermediate 21. m/z (ES+): 395 [M+H]+
Intermediate 62 was prepared using the procedure for intermediate 22. m/z (ES+): 363 [M+H]+
To a solution of (S)-benzyl 2-(2-(2,4-dimethylbenzoyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (7 g, 17.8 mmol) in 80 mL of MeOH was added 1 M HCl (35 mL) and Pd/C 5% (1 g). The reaction was stirred under H2 (5 atm) atmosphere for 12 h. The reaction mixture was filtered through a celite pad and concentrated in high vacuum. The residue was dissolved in MeOH (30 ml) and NaBH3(CN) (1.3 g, 20.5 mmol) was added. The reaction was stirred for 5 h at room temperature and filtered. The filtrate was concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/2) to give the title compound (2.3 g, 53%); m/z (ES+): 245 [M+H]+
To a solution of (1S,8aS)-1-(2,4-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (1.5 g, 6.1 mmol) was added (Boc)2O (2.65 g, 12.2 mmol) at 0° C., the reaction mixture was stirred overnight at 25° C., followed by addition of N1,N1-dimethylethane-1,2-diamine (5 mL). The mixture was stirred at 25° C. for 8 h and quenched by sat. NH4Cl solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were dried over anhydrous Na2SO4, and concentrated in high vacuum to give the title compound (2 g, 95%) which was used in the next step without further purification; m/z (ES+): 345 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(2,4-dimethylphenyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (2 g, 5.8 mmol) in anhydrous THF (60 mL) under N2 atmosphere, was added LiHMDS (1 M in THF, 6.05 mL) at −78° C. The reaction was stirred for 15 min, followed by addition of DMPU (1.8 mL, 15 mmol) and allylbromide (0.52 mL, 6 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C. and the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (2 g, yield: 81%); m/z (ES+): 425 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 7,7-diallyl-1-(2,4-dimethylphenyl)-6-oxohexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (2 g, 4.7 mmol) in DCM (40 mL) at 0° C. was added TFA (8 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (1.2 g, yield: 79%); m/z (ES+): 325 [M+H]+.
To a solution of triphosgene (205 mg, 0.70 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(2,4-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (500 mg, 1.54 mmol), DMAP (40 mg, 0.32 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (10 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (3,5-bis(trifluoromethyl)phenyl)-N-methylmethan amine (514 mg, 2 mmol) and TEA (480 mg, 4.8 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl(aq) solution. The resulting mixture was extracted with EtOAc (2×100 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (80 mg, yield: 9%); m/z (ES+): 588 [M+H]+.
To a solution of triphosgene (65.3 mg, 0.22 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(2,4-dimethylphenyl)hexahydropyrrolo [1,2-a]pyrazin-6(7H)-one (140 mg, 0.43 mmol), DMAP (5.3 mg, 0.043 mmol) and TEA (130.5 mg, 1.29 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl)phenyl)-N-methyl ethanamine (126.5 mg, 0.516 mmol) in EtOAc (2 mL) and TEA (130.5 mg, 1.29 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×100 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (120 mg, yield: 47%); m/z (ES+): 596 [M+H]+.
To a solution of triphosgene (65.3 mg, 0.22 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(2-methoxyphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (140 mg, 0.43 mmol), DMAP (5.3 mg, 0.043 mmol) and TEA (130.5 mg, 1.29 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (139 mg, 0.516 mmol) in EtOAc (2 mL) and TEA (130.5 mg, 1.29 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×100 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (45 mg, yield: 17%); m/z (ES+): 624 [M+H]+.
To a solution of 2-Bromo-1,4-dimethyl-benzene (1.7 g, 12.39 mmol) in THF (40 mL) was added n-BuLi (1.6 M in hexane) (7.52 mL, 12.036 mmol) dropwise over 5 min at −70° C. The reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (2.5 g, yield: 55.6%); m/z (ES+): 361 [M+H]+
To a solution of (S)-tert-butyl 2-(2-(2,5-dimethylbenzoyl)-5-oxopyrrolidin-1-yl)ethyl carbamate (2.5 g, 6.9 mmol) in THF (50 mL) was dropwise added LiAl(OtBu)3H (3.56 g, 14 mmol) at −20° C. Then the mixture was stirred at room temperature for 2 h and then poured into the saturated NH4Cl solution. The resulting suspension was filtered through a celite pad, washed by EtOAc. The filtrate was extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.5 g, yield: 98%); m/z (ES+): 363[M+H]+.
To a solution of tert-butyl 2-((S)-2-((R)-(2,5-dimethylphenyl)(hydroxy)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2.5 g, 6.9 mmol) in DCM (20 mL) was added Et3N (3.54 g, 35 mmol), DMAP (85.6 mg, 0.7 mmol). The resulting solution was cooled to 0° C., and then MsCl (1.18 g, 10.3 mmol) was dropwisely added into the above solution under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution and extracted with DCM (2×30 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (800 mg, yield: 27%); m/z (ES+): 441 [M+H]+.
To a suspension of NaH (60%, 128 mg, 3.2 mmol) in THF (20 mL) was added a solution of (R)—((S)-1-(2-(tert-butoxycarbonyl)ethyl)-5-oxopyrrolidin-2-yl)(2,5-dimethylphenyl) methyl methanesulfonate (700 mg, 1.6 mmol) in THF (10 mL) at 0° C. under N2 atmosphere. The mixture was stirred at room temperature for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (500 mg, yield: 91%); m/z (ES+): 345 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(2,5-dimethylphenyl)-6-oxo-hexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (700 mg, 2.03 mmol) in anhydrous THF (100 mL) under N2 atmosphere, was added LiHMDS (1 M in THF, 3.4 mL) at −78° C. The reaction was stirred for 15 min, followed by addition of DMPU (895 mg, 7 mmol) and allylbromide (316.5 mg, 2.62 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C., the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (600 mg, yield: 70%); m/z (ES+): 425 [M+H]+
To a solution of (1R,8aS)-tert-butyl 7,7-diallyl-1-(2,5-dimethylphenyl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (600 mg, 1.41 mmol) in MeOH (20 mL) was added HCl/MeOH (7 M, 5 mL) at 0° C. The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (180 mg, yield: 95%); m/z (ES+): 325 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1R,8aS)-tert-butyl 7,7-diallyl-1-(2,5-dimethylphenyl)-6-oxo-hexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (162 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (163.2 mg, 0.6 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (120 mg, yield: 39%); m/z (ES+): 622 [M+H]+.
To a solution of triphosgene (44.5 mg, 0.15 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1R,8aS)-tert-butyl 7,7-diallyl-1-(2,5-dimethylphenyl)-6-oxo-hexahydro pyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (97.2 mg, 0.3 mmol), DMAP (3.7 mg, 0.03 mmol) and TEA (92 mg, 0.9 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl) phenyl)-N-methyl ethanamine (91.5 mg, 0.36 mmol) in EtOAc (2 mL) and TEA (92 mg, 0.9 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (100 mg, yield: 56.1%); m/z (ES+): 596 [M+H]+.
To a solution of 1-bromo-2, 3-dimethylbenzene (2.28 g, 12.39 mmol) in THF (40 mL) at −70° C. was dropwise added n-BuLi (1.6 M in hexane, 7.52 mL, 12.0 mmol) over 5 min. The reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.5 g, yield: 59%); m/z (ES+): 361 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(2,3-dimethylbenzoyl)-5-oxopyrrolidin-1-yl)ethyl carbamate (2.5 g, 6.9 mmol) in THF (50 mL) was dropwise added LiAl(OtBu)3H (3.56 g, 14 mmol) at −20° C. Then the mixture was stirred at room temperature for 2 h and quenched with saturated NH4Cl solution. The resulting suspension was filtered through a celite pad, washed by EtOAc. The filtrate was extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (2.5 g, yield: 99.7%); m/z (ES+): 363[M+H]+.
To a solution tert-butyl 2-((S)-2-((R)-(2,3-dimethylphenyl)(hydroxy)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2.5 g, 6.9 mmol), Et3N (3.54 g, 35 mmol) and DMAP (85.6 mg, 0.7 mmol) in DCM (20 mL), was dropwise added MsCl (1.18 g, 10.3 mmol) under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (800 mg, yield: 27%); m/z (ES+): 441 [M+H]+.
To a suspension of NaH (60%, 128 mg, 3.2 mmol) in THF (20 mL) was added a solution of (R)—((S)-1-(2-(tert-butoxycarbonylamino)ethyl)-5-oxopyrrolidin-2-yl)(2,3-dimethyl phenyl)methyl methanesulfonate (700 mg, 1.6 mmol) in THF (10 mL) at 0° C. under N2 atmosphere. The mixture was stirred at room temperature for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (500 mg, yield: 91%); m/z (ES+): 345 [M+H]+.
To a solution of (1S, 8aS)-tert-butyl1-(2,3-dimethylphenyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (300 mg, 0.872 mmol) in anhydrous THF (100 mL) under N2 atmosphere, was added LiHMDS (1 M in THF, 3.4 mL) at −78° C. The reaction was stirred for 15 min, followed by addition of DMPU (895 mg, 7 mmol) and allylbromide (316.5 mg, 2.62 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C., the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (250 mg, yield: 70%); m/z (ES+): 425 [M+H]+.
To a solution of (S)-tert-butyl 7,7-diallyl-1-(2,3-dimethylphenyl)-6-oxohexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (250 mg, 0.59 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (180 mg, yield: 95%); m/z (ES+): 325 [M+H]+.
To a solution of triphosgene (73.5 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(2,3-dimethylphenyl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (162 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.7 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (120 mg, yield: 39%); m/z (ES+): 622 [M+H]+.
To a solution of (1S, 8aS)-tert-butyl1-(2,3-dimethylphenyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (500 mg, 1.45 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (300 mg, yield: 84%); m/z (ES+): 245 [M+H]+.
To a solution of 2-bromo-3-methylpyridine (1.7 g, 9.8 mmol) in THF (40 mL) at −70° C. was dropwise added n-BuLi (1.6 M in hexane, 7.52 mL, 12.0 mmol) over 5 min. The reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (700 mg, yield: 28%); m/z (ES+): 348 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(3-methylpicolinoyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (2.5 g, 6.9 mmol) in THF (50 mL) was portionwise added LiAl(OtBu)3H (700 mg, 2.01 mmol) at −20° C. Then the mixture was stirred at room temperature for 2 h and quenched with saturated NH4Cl solution. The resulting suspension was filtered through a celite pad, washed by EtOAc. The filtrate was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (400 mg, yield: 57.1%); m/z (ES+): 350[M+H]+.
To a solution of tert-butyl 2-((S)-2-((S)-hydroxy(3-methylpyridin-2-yl)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (400 mg, 1.14 mmol), Et3N (3.54 g, 35 mmol) and DMAP (85.6 mg, 0.7 mmol) in DCM (20 mL) was dropwise added MsCl (1.18 g, 10.3 mmol) under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (380 mg, yield: 95%); m/z (ES+): 428 [M+H]+.
To a suspension of NaH (60%, 128 mg, 3.2 mmol) in THF (20 mL) was added a solution of (S)—((S)-1-(2-(tert-butoxycarbonyl)ethyl)-5-oxopyrrolidin-2-yl)(3-methylpyridin-2-yl)methyl methanesulfonate (380 mg, 0.89 mmol) in THF (10 mL) at 0° C. under N2 atmosphere. The mixture was stirred at room temperature for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (200 mg, yield: 53%); m/z (ES+): 331 [M+H]+.
To a solution of (1R,8aS)-tert-butyl 1-(3-methylpyridin-2-yl)-6-oxo-hexahydropyrrolo [1,2-a]pyrazine-2(1H)-carboxylate (200 mg, 0.60 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (180 mg, yield: 95%); m/z (ES+): 232 [M+H]+.
Intermediate 91-93 were prepared using the procedure for intermediate 90.
m/z (ES+): 232 [M+H]+.
m/z (ES+): 232 [M+H]+.
To a solution of 3-bromo-4-methylthiophene (1.25 g, 7.1 mmol) in THF (10 mL) was added n-BuLi(2.5 M) (2.8 ml, 7.1 mmol) at −35° C. The reaction was stirred at the same temperature for 30 min, and added to (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (1.5 g, 5.9 mmol) in 10 ml THF. After stirred for 5 min, the reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc (2×20 mL). The organic layers were dried over anhydrous Na2SO4 and concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=10/1 to 1/1) to give the title compound (800 mg, yield: 38%); m/z (ES+): 353 [M+H]+.
To a solution of (S)-tert-butyl 2-(2-(4-methylthiophene-3-carbonyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (800 mg, 2.3 mmol) in THF (10 mL) was portion-wise added LiAl(OtBu3)H (866 mg, 3.4 mmol) at −20° C. Then the mixture was stirred at room temperature for 2 h and quenched with saturated NH4Cl solution. The resulting suspension was filtered through a celite pad, washed by EtOAc. The filtrate was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (730 mg, yield: 91%); m/z (ES+): 355[M+H]+.
To a solution of tert-butyl 2-((S)-2-((R)-hydroxy(4-methylthiophen-3-yl)methyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (700 mg, 1.98 mmol) and Et3N (2.57 ml, 17.8 mmol) in DCM (15 mL) at 0° C. was dropwise added MsCl (0.31 ml, 3.95 mmol) under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (750 mg, yield: 88%); m/z (ES+): 433 [M+H]+.
To a suspension of NaH (60%, 556 mg, 13.9 mmol) in THF (5 mL) at 0° C. was added solution of (R)—((S)-1-(2-(tert-butoxycarbonylamino)ethyl)-5-oxopyrrolidin-2-yl)(4-methylthiophen-3-yl)methyl methanesulfonate (750 mg, 1.74 mmol) in THF (5 mL). The mixture was stirred at 50° C. for 20 h and quenched with ice-water. The resulting mixture was extracted with EtOAc. The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (300 mg, yield: 51%); m/z (ES+): 337 [M+H]+.
A solution of (1R, 8aS)-tert-butyl 1-(4-methylthiophen-3-yl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (300 mg, 0.89 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h and basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-TLC (DCM/MeOH=10/1) to give the title compound (150 mg, yield: 72%); m/z (ES+): 237 [M+H]+.
Intermediate 99-103 were prepared using the procedure for intermediate 98.
m/z (ES+): 237 [M+H]+.
m/z (ES+): 223 [M+H]+.
m/z (ES+): 237 [M+H]+.
m/z (ES+): 238 [M+H]+.
m/z (ES+): 224[M+H]+.
To a suspension of NaH (60%, 2.5 g, 62.9 mmol) in THF (800 mL), was dropwise added 4-bromo-3-methyl-1H-pyrazole (10 g, 62.9 mmol) in 100 ml THF at 0° C. After stirred at the same temperature for 30 min, BnBr (4.4 ml, 62.9 mmol) was added at 0° C. The reaction mixture was stirred at 15° C. for 1 h and carefully quenched with ice-water. The resulting mixture was extracted with EtOAc (2×150 mL). The combined organic layers were washed with brine (2×100 mL), dried over anhydrous Na2SO4 and then concentrated. The residue was purified by silica gel chromatography (PE/EtOAc=4/1 to 1/1) to give the title compound (6 g, yield: 60%); m/z (ES+): 251 [M+H]+.
A solution of MeI (0.05 mmol), I2 (10 mg) and Mg (2.8 g 120 mmol) in THF (10 mL) was dropwise added over 5 min to a stirred solution of the 1-benzyl-4-bromo-3-methyl-1H-pyrazole (3 g, 12 mmol) in THF (10 mL) at −40° C. under argon. The resulting solution was then stirred for 30 min, and (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (5 g, 19.7 mmol) was added. The reaction mixture was allowed to warm to room temperature and stirred till (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate was consumed. The reaction mixture was quenched with saturated NH4Cl solution and extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The crude residue was purified by column chromatography on silica gel (PE/EtOAc, 4:1) to give the alcohol compound (1.6 g, 30%); m/z (ES+): 427[M+H]+.
To a solution of (S)-tert-butyl 2-(2-(1-benzyl-3-methyl-1H-pyrazole-4-carbonyl)-5-oxopyrrolidin-1-yl)ethylcarbamate (1.5 g, 3.8 mmol) in THF (50 mL) was portion-wise added LiAl(OtBu3)H (3.56 g, 14 mmol) at −20° C. Then the mixture was stirred at room temperature for 2 h and quenched with saturated NH4Cl solution. The resulting suspension was filtered through a celite pad, washed by EtOAc. The filtrate was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (1.2 g, yield: 80%); m/z (ES+): 428[M+H]+.
To a solution of tert-butyl 2-((S)-2-((R)-(1-benzyl-3-methyl-1H-pyrazol-4-yl)(hydroxy)methyl)pyrrolidin-1-yl)ethylcarbamate (1.2 g, 2.8 mmol) and Et3N (2.57 ml, 17.8 mmol) in DCM (20 mL), at 0° C. was dropwise added MsCl (1.18 g, 10.3 mmol) under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl solution. The resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (1.1 g, yield: 89%); m/z (ES+): 507 [M+H]+.
To a suspension of NaH (60%, 128 mg, 3.2 mmol) in THF (20 mL) was added a solution of Methanesulfonic acid (1-benzyl-3-methyl-1H-pyrazol-4-yl)-[1-(2-tert-butoxycarbonyl amino-ethyl)-5-oxo-pyrrolidin-2-yl]-methyl ester (1.2 g, 2.3 mmol) in THF (10 mL) at 0° C. under N2 atmosphere. The mixture was stirred at 50° C. for 20 h and quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL). The combined organic layers were dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel (PE/EtOAc=5/1 to 1/1) to give the title compound (1.2 g, yield: 91%); m/z (ES+): 491 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (402 g, 1 mmol) in MeOH (5 mL) at 0° C. was added HCl/MeOH (7M, 5 mL). The reaction was stirred at room temperature for 3 h until intermediate 6 was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (160 mg, yield: 51%); m/z (ES+): 311 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (70 mg, yield: 31%); m/z (ES+): 608 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (70 mg, yield: 31%); m/z (ES+): 568 [M+H]+.
To a solution of (1S,8aS)-tert-butyl 1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)-6-oxo-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (1.2 g, 2.44 mmol) in anhydrous THF (100 mL) under N2 atmosphere, was added LiHMDS (1 M in THF, 3.4 mL at −78° C. The reaction was stirred for 15 min, followed by addition of DMPU (895 mg, 7 mmol) and allylbromide (316.5 mg, 2.62 mmol). The reaction mixture was stirred at −78° C. for 30 min and allowed to warm to −30° C., and then stirred at this temperature for another 2 h. After quenched with water at −78° C., the mixture was then extracted with EtOAc (2×50 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by silica gel chromatography (PE/EtOAc=2/1 to 1/1) to give the title compound (550 mg, yield: 46%); m/z (ES+): 491 [M+H]+
To a solution of (1S,8aS)-tert-butyl 7,7-diallyl-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (550 mg, 1.12 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7M, 5 mL). The reaction was stirred at room temperature for 3 h and then basified with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was directly used in the next step without further purification (360 mg, yield: 95%); m/z (ES+): 391 [M+H]+.
To a solution of triphosgene (53.4 mg, 0.18 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)hexahydropyrrolo [1,2-a]pyrazin-6(7H)-one (83 mg, 0.36 mmol), DMAP (4.6 mg, 0.036 mmol) and TEA (109.2 mg, 1.08 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3-Ethyl-5-trifluoromethyl-phenyl)-ethyl]-methyl-amine (100 mg, 0.43 mmol) in EtOAc (2 mL) and TEA (109.2 mg, 1.08 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (80 mg, yield: 57%); m/z (ES+): 648 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of (1S,8aS)-7,7-diallyl-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)-N—((R)-1-(3-ethyl-5-(trifluoromethyl)phenyl) ethyl)-N-methyl-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide (80 mg 0.12 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (57 mg, 1.5 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM. The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (40 mg, yield: 50%); m/z (ES+): 696 [M+H]+.
To a solution of triphosgene (44 mg, 0.15 mmol) in EtOAc (2 mL) at 0° C. was added solution of (1S,8aS)-7,7-diallyl-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)hexahydropyrrolo [1,2-a]pyrazin-6(7H)-one (120 mg, 0.31 mmol), DMAP (4 mg, 0.03 mmol) and TEA (90 mg, 0.9 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl)phenyl)ethanamine (100 mg, 0.46 mmol) in EtOAc (2 mL) and TEA (90 mg, 1 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (90 mg, yield: 46%); m/z (ES+): 634 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of (1S,8aS)-7,7-diallyl-1-(1-benzyl-3-methyl-1H-pyrazol-4-yl)-N-methyl-N—((R)-1-(3-methyl-5-(trifluoromethyl) phenyl)ethyl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide (90 mg, 0.14 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (106 mg, 2.8 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM. The organic layer was washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (40 mg, yield: 45%); m/z (ES+): 642 [M+H]+.
To a solution of 1-bromo-3-chloro-2-methylbenzene (2.74 g, 12.39 mmol) in THF (40 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.75 mL, 12.39 mmol) drop wise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2 (1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (3.6 g, yield: 80%); m/z (ES+): 381 [M+H]+.
LiAl(OtBu)3H (3.56 g, 14 mmol) was added portion wisely into intermediate 118 (3.6 g, 9.45 mmol) in THF (50 mL) at −20° C. Then the mixture was stirred at room temperature. 2 h later, intermediate 2 was completely consumed. The whole solution was poured into the saturated solution of potassium sodium tartrate. 30 min later, the suspension was filtered through a celite pad, washed by EtOAc for several times. The filtration was extracted with EtOAc (2×20 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (2.9 g, yield: 80%); m/z (ES+): 383[M+H]+.
To a solution of intermediate 119 (2.9 g, 7.6 mmol) and Et3N (3.54 g, 35 mmol) in DCM at 0° C. was added MsCl (1.18 g, 10.3 mmol) dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (2.7 g, yield: 77%); m/z (ES+): 461 [M+H]+.
Intermediate 120 (2.7 g, 5.8 mmol) in THF (10 mL) was added into a suspension of NaH (60%, 468 mg, 11.7 mmol) in THF (20 mL) at 0° C. under N2 atmosphere. The mixture was stirred at RT for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), the combined organic layers were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (600 mg, yield: 29%); m/z (ES+): 365 [M+H]+.
To a solution of intermediate 121 (600 mg, 1.64 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7M, 5 mL). The reaction was stirred at room temperature until the starting materials was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (305 mg, yield: 70%); m/z (ES+): 265 [M+H]+.
To a solution of 1-bromo-2-ethylbenzene (2.29 g, 12.39 mmol) in THF (40 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.52 mL, 12.036 mmol) drop wise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2 (1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 10 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1.3 g, yield: 30%); m/z (ES+): 361 [M+H]+.
To a stirred solution of intermediate 123 (500 mg, 1.39 mmol) in dioxane (5 mL) was treated with saturated solution of HCl in dioxane (10 mL) drop wise for 10 min and later stirred at room temperature for 24 h. Solvent was evaporated under reduced pressure and the residue was washed with Et2O, used for next step without any further purification (361 mg, yield: 100%); m/z (ES+): 261 [M+H]+.
To a stirred solution of intermediate 124 (361 mg, 1.39 mmol) in MeOH (1.2 L) was treated with cat. AcOH (0.5 mL) and NaCNBH3 (627 mg, 5.56 mmol) at 0° C. and allowed to RT and maintained for 16 h. On completion, the solvent was removed under reduced pressure; sat.NaHCO3 solution was added to the residue and extracted with DCM, washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (200 mg, yield: 59%); m/z (ES+): 245 [M+H]+.
To a solution of 1-bromo-2-chlorobenzene (2.38 g, 12.39 mmol) in toluene (40 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.52 mL, 12.04 mmol) drop wise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 20 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2 (1H)-carboxylate (3 g, 11.8 mmol) in toluene (40 mL). After stirred at −70° C. for 10 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1 g, yield: 23%); m/z (ES+): 367 [M+H]+.
To a stirred solution of intermediate 126 (728 mg, 2.73 mmol) in dioxane (5 mL) was treated with saturated solution of HCl in dioxane drop wise for 10 min and later stirred at room temperature for 24 h. Solvent was evaporated under reduced pressure and the residue was washed with Et2O, used for next step without any further purification; m/z (ES+): 267 [M+H]+.
To a stirred solution of intermediate 127 (728 mg, 2.73 mmol) in MeOH (1.2 L) was treated with cat. AcOH (0.5 mL) and NaCNBH3 (860 mg, 13.65 mmol) at 0° C. and allowed to RT and maintained for 16 h. On completion, the solvent was removed under reduced pressure; sat.NaHCO3 solution was added to the residue and extracted with DCM, washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (125 mg, yield: 18%); m/z (ES+): 251 [M+H]+.
To a solution of 1-bromo-2-vinylbenzene (4.03 g, 22 mmol) in THF (15 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (14.45 mL, 23.1 mmol) dropwisely by syringe along the wall of the flask. After adding, the reaction was stirred for 30 min at this temperature. Then (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (7.0 g, 27 mmol) was added into the above solution in one portion. After stirred at −40° C. for 15 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (5.6 g, yield: 71%); m/z (ES+): 359 [M+H]+.
LiAl(OtBu)3H (5.97 g, 23.45 mmol) was added portion wisely into intermediate 129 (5.6 g, 15.6 mmol) in THF (50 mL) at −20° C. Then the solution was slowly warmed to room temperature. 2 h later, the starting material was completely consumed; the whole solution was poured into the saturated NH4Cl, the suspension was filtered through a celite pad, washed by EtOAc. The filtration was extracted with EtOAc (2×100 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (3.86 g, yield: 68.5%); m/z (ES+): 361 [M+H]+.
Intermediate 130 (3.86 g, 10.72 mmol), Et3N (5.41 g, 53.6 mmol) in DCM (20 mL), then the mixture was cooled to 0° C. MsCl (1.842 g, 16.08 mmol) was added into the above solution drop wisely under N2 atmosphere. The reaction was stirred overnight at 30° C. After most of starting material was consumed, the reaction was quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (3.1 g, yield: 66%); m/z (ES+): 439 [M+H]+.
Intermediate 131 (3.1 g, 7.1 mmol) in THF (10 mL) was added into a suspension of NaH (60%, 128 mg, 14.15 mmol) in THF (20 mL) at 0° C. under N2 atmosphere. The mixture was stirred at 40′C overnight and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (617 mg, yield: 26%); m/z (ES+): 343 [M+H]+.
To a solution of Intermediate 132 (617 mg, 1.8 mmol) in MeOH (15 mL) at 0° C. was added HCl/MeOH (7M, 15 mL). The reaction was stirred at room temperature for 3 h until the starting material was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (500 mg, yield: 90%); m/z (ES+): 243 [M+H]+.
To a solution of triphosgene (489 mg, 1.662 mmol) in EtOAc (10 mL) at 0° C. was added (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (904 mg, 3.324 mmol), DMAP (41 mg, 0.03324 mmol) and TEA (840 mg, 8.3 mmol). The mixture was stirred for 2 h at RT, followed by addition of intermediate 133 (670 mg, 2.77 mmol) in EtOAc (2 mL) and TEA (840 mg, 8.3 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with EtOAc; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (450 mg, yield: 31%); m/z (ES+): 540[M+H]+.
To a solution of 3,4-dimethylthiophene (2.5 g, 10 mmol) in THF (20 mL) at −70° C. was added LDA (1.6 M in hexane) (7.52 mL, 12.036 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (1.8 g, 10 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1.5 g, yield: 20%); m/z (ES+): 367 [M+H]+.
LiAl(OtBu)3H (1 g, 2.8 mmol) was added portion wisely into intermediate 135 (1.3 g, 5 mmol) in THF (20 mL) at −20° C. Then the mixture was stirred at room temperature. 2 h later, the starting material was completely consumed; the whole solution was poured into the saturated NH4Cl, the suspension was filtered through a celite pad, washed by EtOAc. The filtration was extracted with EtOAc (2×20 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1 g, yield: 100%); m/z (ES+): 369 [M+H]+.
Intermediate 136 (1 g, 2.8 mmol) in DCM (10 mL), then Et3N (707 mg, 7 mmol), DMAP (18 mg, 0.15 mmol) were added. The resulting solution was cooled to 0° C., and then MsCl (400 mg, 3.5 mmol) was added into the above solution dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (980 mg, yield: 103%); m/z (ES+): 350 [M+H]+.
To a solution of Intermediate 137 (980 g, 2.8 mmol) in MeOH (15 mL) at 0° C. was added HCl/MeOH (7M, 15 mL). The reaction was stirred at room temperature for 3 h until intermediate 6 was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (600 mg, yield: 90%); m/z (ES+): 251[M+H]+.
To 3-methoxy-2-methyl aniline (2.74 g, 20 mmol), water (6 mL) and 48% aqueous solution of hydrobromic acid (2.4 mL) were added. To this sodium nitrite (2.76 g, 40 mmol) in water (1.20 mL) was added dropwise at 0° C. for 10 minutes. After adding acetone (24 mL) to the reaction mixture, copper bromide (5.64 g, 40 mmol) was added at 0° C. and stirred for 3 hours. The reaction mixture was warmed to room temperature and concentrated. The residue was diluted with dichloromethane and washed with a mixture of saturated aqueous sodium hydrogen carbonate and aqueous ammonia. The organic layer was dried over anhydrous sodium sulfate and the filtrate was concentrated to obtain the title compound (2.5 g, yield: 62%); m/z (ES+): 201[M+H]+.
To a solution of intermediate 139 (2.5 g, 12.4 mmol) in THF (30 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.8 mL, 12.4 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (2.0 g, 12 mmol) in THF (30 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (2 g, yield: 45%); m/z (ES+): 377 [M+H]+.
LiAl(OtBu)3H (2 g, 8 mmol) was added portion wisely into intermediate 140 (2 g, 5.3 mmol) in THF (30 mL) at −20° C. Then the mixture was stirred at room temperature. 2 h later, the starting material was completely consumed; the whole solution was poured into the saturated NH4Cl, the suspension was filtered through a celite pad, washed by EtOAc. The filtration was extracted with EtOAc (2×50 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (2 g, yield: 100%); m/z (ES+): 379 [M+H]+.
Intermediate 141 (2.3 g, 6.1 mmol) in DCM (20 mL), then Et3N (3.54 g, 35 mmol), DMAP (85.6 mg, 0.7 mmol) were added. The resulting solution was cooled to 0° C., and then MsCl (1.18 g, 10.3 mmol) was added into the above solution dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1.5 g, yield: 67%); m/z (ES+): 361 [M+H]+.
To a solution of intermediate 142 (1.1 g, 3 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7M, 15 mL). The reaction was stirred at room temperature for 3 h until intermediate 6 was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (811 mg, yield: 95%); m/z (ES+): 261 [M+H]+.
To a solution of 4-bromo-2,3-dihydro-1H-inden-1-one (2 g, 10 mmol) in DCM (10 mL) was added TFA, then cooled to −20° C. Et3SiH (15 mL, 1N in THF, 15 mmol) was added drop wise. After stirring for 2 h at RT the reaction mixture was poured into NH4Cl aqueous and extracted with DCM (10 mL*2), washed with brine (10 mL*2), dried by MgSO4, concentrated, purified by FC to afford compound (1.7 g, yield: 85%); m/z (ES+): 197 [M+H]+.
To a solution of intermediate 144 (1.7 g, 12.39 mmol) in THF (40 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.52 mL, 12.036 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (3 g, 11.8 mmol) in THF (40 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1.6 g, yield: 41%); m/z (ES+): 373 [M+H]+.
LiAl(OtBu)3H (1.7 g, 6.5 mmol) was added portion wisely into intermediate 145 (1.6 g, 4.3 mmol) in THF (50 mL) at −20° C. Then the mixture was stirred at room temperature. 2 h later, the starting material was completely consumed. The whole solution was poured into the saturated NH4Cl, the suspension was filtered through a celite pad, washed by EtOAc. The filtration was extracted with EtOAc (2×20 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (1.5 g, yield: 95%); m/z (ES+): 375 [M+H]+.
Intermediate 146 (1.5 g, 4 mmol) in DCM (20 mL), then Et3N (0.7 g, 4 mmol), DMAP (85.6 mg, 0.7 mmol) were added. The resulting solution was cooled to 0° C., and then MsCl (0.94 g, 8 mmol) was added into the above solution dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (600 mg, yield: 40%); m/z (ES+): 453 [M+H]+.
Intermediate 147 (600 mg, 1.1 mmol) in THF (10 mL) was added into a suspension of NaH (60%, 128 mg, 3.2 mmol) in THF (20 mL) at 0° C. under N2 atmosphere. The mixture was stirred at RT for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (400 mg, yield: 66.7%); m/z (ES+): 357 [M+H]+.
To a solution of intermediate 148 (400 mg, 1.12 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7M, 5 mL). The reaction was stirred at room temperature for 3 h until intermediate 6 was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (250 mg, yield: 95%); m/z (ES+): 257 [M+H]+.
To a solution of 2-bromo-1-fluoro-3-methylbenzene (2.5 g, 10 mmol) in THF (20 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (6.9 mL, 11.0 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (2.07 g, 11.0 mmol) in THF (10 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (2.2 g, yield: 40%); m/z (ES+): 365 [M+H]+.
LiAl(OtBu)3H (4.2 g, 16.5 mmol) was added portion wisely into intermediate 150 (2 g, 5.5 mmol) in THF (15 mL) at −20° C. Then the mixture was stirred at room temperature. 2 h later, (S)-tert-butyl2-(2-(2-fluoro-6-methylbenzoyl)-5-oxopyrrolidin-1-yl) ethylcarbamate was completely consumed; the whole solution was poured into the saturated NH4Cl, the suspension was filtered through a celite pad, washed by EtOAc. The filtration was extracted with EtOAc (2×20 mL) washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (0.9 g, yield: 45%); m/z (ES+): 367[M+H]+.
Intermediate 151 (0.9 g, 2.46 mmol) in DCM (20 mL), then Et3N (746 g, 7.38 mmol) was added. The resulting solution was cooled to 0° C., and then MsCl (421 mg, 3.69 mmol) was added into the above solution dropwisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and then quenched with saturated NH4Cl and extracted with DCM (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (300 mg, yield: 27.4%); m/z (ES+): 445 [M+H]+.
Intermediate 152 (300 mg, 0.68 mmol) in THF (5 mL) was added into a suspension of NaH (60%, 49 mg, 2.04 mmol) in THF (2 mL) at 0° C. under N2 atmosphere. The mixture was stirred at RT for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (150 mg, yield: 63.5%); m/z (ES+): 349 [M+H]+.
To a solution of intermediate 153 (70 mg, 0.20 mmol) in MeOH (2 mL) at 0° C. was added HCl/MeOH (7M, 5 mL). The reaction was stirred at room temperature for 3 h until intermediate 5 was completely consumed. MeOH was removed under reduced pressure, the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (45 mg, yield: 90%); m/z (ES+): 249 [M+H]+.
To a solution of 1-bromo-2-chloro-4-fluorobenzene (2.5 g, 12.0 mmol) in toluene (10 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (7.5 mL, 12.0 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (2.8 g, 10.9 mmol) in toulene (10 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (850.0 mg, yield: 20%); m/z (ES+): 385 [M+H]+.
To a solution of intermediate 155 (900.0 mg, 2.3 mmol) in dioxane (20 mL) at 0° C. was added HCl/dioxane (6 M, 10 mL). The reaction was stirred at room temperature for 3 h until intermediate 2 was completely consumed. Dioxane was removed under reduced pressure, the residue was directly used in the next step without further purification (621.0 mg, yield: 95%); m/z (ES+): 285 [M+H]+.
Intermediate 156 (621.0 mg, 2.2 mmol) was dissolved in MeOH (10 mL) and then NaBH3CN (277.0 mg, 4.4 mmol) and HOAc (1 d) were added to above solution at 0° C. under N2 atmosphere. The mixture was stirred at RT for 20 h and then quenched with saturated NH4Cl. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (84 mg, yield: 14%); m/z (ES+): 269 [M+H]+.
To a solution of 1-bromo-4-fluorobenzene (1.9 g, 11.0 mmol) in THF (10 mL) at −70° C. was added n-BuLi (1.6 M in hexane) (6.9 mL, 11.0 mmol) dropwise over 5 min by syringe along the wall of the flask. After adding, the reaction was stirred at this temperature for 30 min. Then the resulting solution was added into a solution of (S)-tert-butyl 1,6-dioxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxylate (2.5 g, 10.0 mmol) in THF (10 mL). After stirred at −70° C. for 5 min, the reaction mixture was quenched with saturated NH4Cl and extracted with EtOAc (2×20 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (960.0 mg, yield: 27%); m/z (ES+): 351 [M+H]+.
LiAl(OtBu)3H (1.4 g, 5.6 mmol) was added portion wisely into intermediate 158 (960.0 mg, 2.8 mmol) in THF (10 mL) at −20° C. Then the mixture was stirred at room temperature. After two hours, intermediate 2 was completely consumed; the whole solution was poured into the saturated NH4Cl. The suspension was filtered through a celite pad, and washed by EtOAc. The filtration was extracted with EtOAc (2×20 mL) and the organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (770.0 mg, yield: 78.0%); m/z (ES+): 353 [M+H]+.
Intermediate 159 (770.0 mg, 2.2 mmol) was dissolved in DCM (15 mL), and then Et3N (2.2 g, 22.0 mmol) was added. The resulting solution was cooled to 0° C., and then MsCl (374.0 g, 3.3 mmol) was added into the above solution drop wisely under N2 atmosphere. The reaction was stirred for 20 h at room temperature and quenched with saturated NH4Cl and extracted with DCM (2×20 mL). The organic layer was washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (860.0 mg, yield: 91%); m/z (ES+): 431 [M+H]+.
Intermediate 160 (860.0 mg, 2.0 mmol) in THF (10 mL) was added into a suspension of NaH (60%, 160.0 mg, 4.0 mmol) in THF (20 mL) at 0° C. under N2 atmosphere. The mixture was stirred at RT for 20 h and then quenched with ice-water. The resulting mixture was extracted with EtOAc (2×30 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by silica gel chromatography to give the title compound (100.0 mg, yield: 15%); m/z (ES+): 335 [M+H]+.
To a solution of intermediate 161 (100.0 mg, 0.3 mmol) in MeOH (20 mL) at 0° C. was added HCl/MeOH (7 M, 5 mL). The reaction was stirred at room temperature for 3 h until intermediate 5 was completely consumed. MeOH was removed under reduced pressure, and the residue was neutralized with 1 M aqueous NaOH solution to pH=10. The mixture was extracted with EtOAc (2×20 mL), washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was directly used in the next step without further purification (50.0 mg, yield: 71%); m/z (ES+): 235 [M+H]+.
To a solution of triphosgene (65 mg, 0.22 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 3 (mixture of trans, 120 mg, 0.49 mmol), DMAP (4 mg, 0.035 mmol) and TEA (150 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (266 mg, 0.98 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound 1 (10 mg, 4%) and compound 2 (10 mg, 4%).
compound 1: 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.53 (s, 2H), 7.26 (s, 1H), 7.10 (d, J=7.8 Hz, 1H), 7.00 (s, 1H), 6.92 (d, J=7.6 Hz, 1H), 5.57 (d, J=6.8 Hz, 1H), 4.14 (d, J=12.7 Hz, 1H), 4.02 (d, J=9.8 Hz, 1H), 3.72 (dd, J=16.0, 7.5 Hz, 1H), 3.26 (d, J=11.7 Hz, 1H), 3.10 (t, J=12.3 Hz, 1H), 2.96 (dd, J=11.8, 9.4 Hz, 1H), 2.68 (s, 3H), 2.48 (s, 3H), 2.43-2.36 (m, 1H), 2.33 (d, J=8.9 Hz, 1H), 2.28 (s, 3H), 1.91-1.78 (m, 1H), 1.70-1.59 (m, 1H), 1.44 (d, J=6.8 Hz, 3H); m/z (ES+): 542 [M+H]+
compound 2: 1H NMR (400 MHz, CDCl3) δ 7.71 (s, 1H), 7.36 (s, 2H), 7.11 (d, J=7.8 Hz, 1H), 6.99 (s, 1H), 6.96 (d, J=8.0 Hz, 1H), 5.59 (q, J=7.0 Hz, 1H), 4.16 (d, J=12.8 Hz, 1H), 4.02 (d, J=9.8 Hz, 1H), 3.78-3.58 (m, 1H), 3.31 (d, J=12.2 Hz, 1H), 3.12 (t, J=12.4 Hz, 1H), 2.90 (td, J=12.0, 3.0 Hz, 1H), 2.81 (s, 3H), 2.46 (d, J=8.3 Hz, 3H), 2.40 (dd, J=10.0, 3.8 Hz, 1H), 2.35-2.30 (m, 1H), 2.28 (s, 3H), 1.85 (ddd, J=16.7, 13.1, 3.9 Hz, 2H), 1.69 (ddd, J=22.5, 13.2, 9.3 Hz, 3H), 1.51 (d, J=7.1 Hz, 3H), 1.24 (t, J=7.0 Hz, 1H). m/z (ES+): 542 [M+H]+.
To a solution of triphosgene (65 mg, 0.22 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 3 (mixture of trans, 120 mg, 0.49 mmol), DMAP (4 mg, 0.035 mmol) and TEA (209 μL, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of 1-(3,5-bis(trifluoromethyl)phenyl)-N-methylmethanamine (251 mg, 0.98 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound 3 (10 mg, 4%). 1H NMR (600 MHz, CDCl3) δ 7.74 (s, 1H), 7.38 (s, 2H), 7.14 (d, J=7.8 Hz, 1H), 6.99 (s, 1H), 6.95 (d, J=7.8 Hz, 1H), 4.69 (d, J=15.5 Hz, 1H), 4.37 (d, J=15.4 Hz, 1H), 4.16 (d, J=12.9 Hz, 1H), 4.03 (d, J=9.9 Hz, 1H), 3.70 (dd, J=16.9, 7.3 Hz, 1H), 3.36 (d, J=11.7 Hz, 1H), 3.18-3.08 (m, 1H), 2.99-2.90 (m, 4H), 2.47 (s, 3H), 2.42 (ddd, J=17.0, 10.0, 3.7 Hz, 1H), 2.34 (dd, J=17.7, 8.9 Hz, 1H), 2.28 (s, 3H), 1.86 (ddd, J=13.2, 12.1, 3.8 Hz, 1H), 1.67 (ddd, J=17.3, 13.2, 9.3 Hz, 1H); m/z (ES+): 528 [M+H]+
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (1 mL) at 0° C. was added solution of intermediate 3 (mixture of trans, 100 mg, 0.32 mmol), DMAP (4 mg, 0.035 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of 1-(3,5-bis(trifluoromethyl)phenyl)-N-methylmethanamine (251 mg, 0.98 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound 4 (76 mg, yield: 38%) and compound 5 (62 mg, yield: 28%).
Compound 4: 1H NMR (400 MHz, CDCl3) δ 7.25 (s, 1H), 7.16 (d, J=7.8 Hz, 1H), 7.00 (dd, J=19.0, 7.2 Hz, 3H), 6.74 (s, 1H), 5.53 (q, J=6.9 Hz, 1H), 4.15 (d, J=12.8 Hz, 1H), 4.02 (d, J=9.8 Hz, 1H), 3.66 (dd, J=16.3, 7.4 Hz, 1H), 3.29 (d, J=11.9 Hz, 1H), 3.10 (t, J=12.4 Hz, 1H), 2.90 (dt, J=11.1, 5.5 Hz, 1H), 2.77 (s, 3H), 2.48 (d, J=13.1 Hz, 3H), 2.44-2.31 (m, 2H), 2.29 (s, 3H), 2.24 (s, 3H), 1.85 (dd, J=12.2, 8.3 Hz, 1H), 1.64-1.61 (m, 1H), 1.45 (d, J=7.1 Hz, 3H); m/z (ES+): 488 [M+H]+
Compound 5 1H NMR (400 MHz, CDCl3) δ 7.31 (s, 1H), 7.27 (s, 1H), 7.12 (d, J=7.8 Hz, 1H), 7.01 (s, 2H), 6.92 (d, J=7.7 Hz, 1H), 5.50 (q, J=6.8 Hz, 1H), 4.12 (d, J=12.8 Hz, 1H), 4.03 (d, J=9.9 Hz, 1H), 3.69 (dd, J=16.6, 7.2 Hz, 1H), 3.28 (d, J=11.9 Hz, 1H), 3.10 (t, J=12.3 Hz, 1H), 2.94 (td, J=11.8, 3.0 Hz, 1H), 2.62 (s, 3H), 2.50 (s, 3H), 2.43-2.35 (m, 1H), 2.33 (s, 3H), 2.29 (s, 3H), 1.84 (ddd, J=16.8, 13.0, 3.9 Hz, 1H), 1.70-1.60 (m, 2H), 1.40 (d, J=6.6 Hz, 3H); m/z (ES+): 488 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 7 (200 mg, 0.32 mmol) in DCM (30 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (1.2 g, 32 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM 30 (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound 6 (15 mg, 7%) and compound 7 (12 mg, 6%).
Compound 6 1H NMR (400 MHz, CDCl3) δ 7.71 (s, 1H), 7.35 (s, 2H), 7.11 (d, J=7.8 Hz, 1H), 7.00-6.89 (m, 2H), 5.57 (q, J=6.8 Hz, 1H), 4.11 (d, J=12.6 Hz, 1H), 4.00 (d, J=9.7 Hz, 1H), 3.76 (ddt, J=29.0, 23.0, 8.4 Hz, 4H), 3.55 (dt, J=39.9, 17.1 Hz, 1H), 3.33 (d, J=12.0 Hz, 2H), 3.13 (td, J=12.5, 3.2 Hz, 1H), 2.93 (dd, J=12.1, 9.0 Hz, 1H), 2.80 (s, 3H), 2.48 (s, 3H), 2.27 (s, 3H), 1.89 (t, J=5.6 Hz, 2H), 1.82 (dd, J=13.5, 7.0 Hz, 2H), 1.74-1.66 (m, 2H), 1.50 (d, J=7.1 Hz, 3H); m/z (ES+): 630 [M+H]+
Compound 7 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.52 (s, 2H), 7.11 (d, J=7.8 Hz, 1H), 7.00 (s, 1H), 6.93 (d, J=7.7 Hz, 1H), 5.57 (d, J=6.9 Hz, 1H), 4.10 (d, J=12.7 Hz, 1H), 4.01 (d, J=9.8 Hz, 1H), 3.88-3.65 (m, 5H), 3.64-3.54 (m, 1H), 3.29 (d, J=11.5 Hz, 1H), 3.12 (t, J=12.3 Hz, 1H), 3.00 (t, J=10.7 Hz, 1H), 2.67 (s, 3H), 2.49 (s, 4H), 2.31 (d, J=17.3 Hz, 4H), 1.85 (ddd, J=19.1, 11.7, 6.0 Hz, 5H), 1.70 (dd, J=12.6, 7.9 Hz, 6H), 1.44 (d, J=6.7 Hz, 3H); m/z (ES+): 630 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 8 (mixture of trans, 180 mg, 0.32 mmol) in DCM (30 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (1.2 g, 32 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (22 mg, 12%). 1H NMR (400 MHz, CDCl3) δ 7.33-7.23 (m, 1H), 7.20-7.09 (m, 1H), 6.97 (dt, J=22.5, 8.9 Hz, 1H), 6.73 (s, 1H), 5.51 (dd, J=14.2, 7.1 Hz, 1H), 4.14-3.95 (m, 1H), 3.85-3.63 (m, 2H), 3.61-3.50 (m, 1H), 3.31 (d, J=11.7 Hz, 1H), 3.12 (t, J=12.3 Hz, 1H), 3.07-2.82 (m, 1H), 2.76 (s, 1H), 2.61 (s, 1H), 2.47 (d, J=21.7 Hz, 1H), 2.28 (t, J=17.6 Hz, 2H), 1.91-1.77 (m, 1H), 1.76-1.63 (m, 1H), 1.42 (dd, J=19.8, 6.4 Hz, 1H); m/z (ES+): 576 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 9 (mixture of trans, 180 mg, 0.32 mmol) in DCM (30 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (1.2 g, 32 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (60 mg, 30%). 1H NMR (400 MHz, CDCl3) δ 7.74 (s, 1H), 7.37 (s, 2H), 7.15 (d, J=7.8 Hz, 1H), 7.02-6.92 (m, 2H), 4.69 (d, J=15.5 Hz, 1H), 4.37 (d, J=15.3 Hz, 1H), 4.10 (d, J=12.7 Hz, 1H), 4.02 (d, J=9.8 Hz, 1H), 3.82-3.67 (m, 5H), 3.60-3.49 (m, 1H), 3.39 (d, J=11.9 Hz, 1H), 3.15 (td, J=12.6, 2.9 Hz, 2H), 3.02-2.87 (m, 5H), 2.48 (s, 4H), 2.32 (d, J=33.5 Hz, 4H), 1.93-1.78 (m, 4H), 1.69 (dt, J=12.0, 6.9 Hz, 2H). m/z (ES+): 616 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 11 (trans mixture, 66 mg, 0.11 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (44 mg, 1.1 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. The reaction was quenched with water and the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound 10 (20 mg, yield: 30%) and compound 11 (17 mg, yield: 23%).
Compound 10). 1H NMR (400 MHz, CDCl3) δ 7.51 (s, 1H), 7.21 (dd, J=9.3, 7.1 Hz, 3H), 6.93-6.81 (m, 2H), 5.43 (t, J=24.7 Hz, 1H), 4.10 (d, J=12.7 Hz, 1H), 4.01 (d, J=9.8 Hz, 1H), 3.84-3.68 (m, 4H), 3.59 (s, 1H), 3.40 (s, 1H), 3.29 (d, J=11.7 Hz, 1H), 3.18-3.08 (m, 1H), 2.98 (dd, J=11.8, 9.6 Hz, 1H), 2.66 (s, 3H), 2.53 (s, 3H), 1.93-1.81 (m, 5H), 1.74-1.67 (m, 2H), 1.40 (d, J=6.3 Hz, 3H); m/z (ES+): 600 [M+H]+.
Compound 11 1H NMR (400 MHz, CDCl3) δ 7.45 (s, 1H), 7.21 (d, J=6.3 Hz, 1H), 7.09 (s, 1H), 6.94-6.85 (m, 3H), 5.50 (d, J=6.8 Hz, 1H), 4.12 (d, J=12.3 Hz, 1H), 4.00 (d, J=9.6 Hz, 1H), 3.79-3.66 (m, 4H), 3.61-3.55 (m, 1H), 3.31 (d, J=11.2 Hz, 1H), 3.12 (t, J=11.2 Hz, 1H), 2.94 (d, J=10.5 Hz, 1H), 2.80 (s, 3H), 2.64 (d, J=16.5 Hz, 1H), 2.53 (s, 3H), 1.87 (dd, J=16.8, 6.1 Hz, 4H), 1.71 (dd, J=13.3, 5.5 Hz, 2H), 1.47 (d, J=6.9 Hz, 3H); m/z (ES+): 600 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 12 (mixture of trans, 100 mg, 0.17 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (44 mg, 1.1 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. The reaction was quenched with water and the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound 12 (16 mg, yield: 16%) and compound 13 (14 mg, yield: 15%).
Compound 12 1H NMR (400 MHz, CDCl3) δ 7.24 (d, J=12.8 Hz, 2H), 7.02 (s, 1H), 6.93-6.78 (m, 2H), 6.71 (s, 1H), 5.50 (d, J=6.9 Hz, 1H), 4.10 (d, J=12.7 Hz, 1H), 3.99 (d, J=9.7 Hz, 1H), 3.84-3.66 (m, 4H), 3.58 (d, J=5.7 Hz, 1H), 3.29 (d, J=12.2 Hz, 1H), 3.10 (t, J=11.4 Hz, 1H), 2.91 (t, J=10.8 Hz, 1H), 2.74 (s, 3H), 2.53 (s, 3H), 2.24 (s, 3H), 1.94-1.78 (m, 6H), 1.73 (d, J=9.4 Hz, 3H), 1.44 (d, J=6.8 Hz, 3H); m/z (ES+): 600 [M+H]+.
compound 13. 1H NMR (400 MHz, CDCl3) δ 7.31 (s, 1H), 7.27-7.21 (m, 1H), 7.15 (s, 1H), 6.97 (s, 1H), 6.88 (d, J=9.7 Hz, 1H), 6.80 (t, J=7.8 Hz, 1H), 5.45 (d, J=6.9 Hz, 1H), 4.04 (dd, J=26.8, 11.4 Hz, 2H), 3.73 (ddd, J=20.2, 13.2, 6.0 Hz, 4H), 3.61-3.53 (m, 1H), 3.28 (d, J=11.7 Hz, 1H), 3.10 (t, J=12.4 Hz, 1H), 2.96 (t, J=10.7 Hz, 1H), 2.62 (s, 3H), 2.53 (s, 3H), 2.33 (s, 3H), 1.90-1.80 (m, 4H), 1.71-1.64 (m, 2H), 1.37 (d, J=5.7 Hz, 3H); m/z (ES+): 580 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 13 (mixture of trans, 200 mg, 0.32 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (243 mg, 6.4 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound 14 (10 mg, yield: 4.9%) and compound 15 (10 mg, yield: 4.9%).
Compound 14. 1H NMR (400 MHz, CDCl3) δ 7.73 (s, 1H), 7.38-7.18 (m, 3H), 6.93-6.76 (m, 2H), 5.57 (d, J=6.6 Hz, 1H), 4.07 (dd, J=44.3, 11.2 Hz, 2H), 3.91-3.48 (m, 5H), 3.34 (d, J=11.8 Hz, 1H), 3.13 (t, J=11.2 Hz, 1H), 3.02-2.70 (m, 4H), 2.53 (s, 3H), 1.83 (dd, J=41.1, 27.5 Hz, 7H), 1.52 (d, J=6.6 Hz, 3H); m/z (ES+): 634 [M+H]+.
Compound 15. 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.53 (s, 2H), 7.24 (d, J=19.4 Hz, 2H), 7.02-6.67 (m, 2H), 5.54 (s, 1H), 4.06 (dd, J=37.9, 10.7 Hz, 2H), 3.89-3.50 (m, 5H), 3.28 (d, J=10.0 Hz, 1H), 3.05 (dd, J=37.8, 10.5 Hz, 2H), 2.61 (d, J=66.8 Hz, 7H), 1.79 (dd, J=60.9, 12.4 Hz, 7H), 1.43 (s, 3H); m/z (ES+): 634 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 16 (mixture of trans, 65 mg, 0.1 mmol) in DCM (10 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (60 mg, 1.5 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by prep-HPLC to give the title compound (12 mg, 20%). 1H NMR (400 MHz, CDCl3) δ 7.33 (s, 1H), 7.17 (d, J=8.8 Hz, 2H), 7.04 (s, 1H), 6.72 (d, J=2.5 Hz, 1H), 6.67 (dd, J=8.5, 2.3 Hz, 1H), 5.50 (q, J=6.8 Hz, 1H), 4.08 (d, J=12.8 Hz, 1H), 3.98 (d, J=9.8 Hz, 1H), 3.88-3.65 (m, 7H), 3.59 (dt, J=11.7, 6.0 Hz, 1H), 3.28 (d, J=11.5 Hz, 1H), 3.11 (td, J=12.4, 3.2 Hz, 1H), 2.97 (dd, J=11.9, 9.1 Hz, 1H), 2.65-2.57 (m, 5H), 2.52 (s, 3H), 1.88 (dd, J=10.4, 4.6 Hz, 3H), 1.85-1.80 (m, 2H), 1.76-1.61 (m, 4H), 1.38 (d, J=6.1 Hz, 3H), 1.20 (t, J=7.6 Hz, 3H); m/z (ES+): 606 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of Intermediate 16 (82 mg, 0.15 mmol) in DCM (10 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (60 mg, 1.5 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by prep-HPLC to give the title compound (33.0 mg, yield: 40%). 1H NMR (400 MHz, CDCl3) δ 7.31 (s, 1H), 7.27 (s, 1H), 7.16 (d, J=8.5 Hz, 2H), 6.99 (s, 1H), 6.72 (d, J=2.5 Hz, 1H), 6.68-6.61 (m, 1H), 5.49 (q, J=6.7 Hz, 1H), 4.08 (d, J=12.7 Hz, 1H), 3.98 (d, J=9.8 Hz, 1H), 3.84 (dd, J=11.7, 5.9 Hz, 1H), 3.78 (s, 3H), 3.71 (d, J=5.7 Hz, 1H), 3.60 (dd, J=11.6, 5.8 Hz, 1H), 3.29 (d, J=12.1 Hz, 1H), 3.11 (td, J=12.3, 3.3 Hz, 1H), 2.97 (dd, J=11.9, 9.0 Hz, 1H), 2.62 (s, 3H), 2.52 (s, 3H), 2.33 (s, 3H), 1.89 (t, J=5.9 Hz, 2H), 1.83 (dd, J=8.2, 5.6 Hz, 2H), 1.81-1.62 (m, 5H), 1.39 (d, J=6.3 Hz, 3H); m/z (ES+): 592 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (1 mL) at 0° C. was added solution of intermediate 17 (mixture of trans, 100 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 2 h, followed by addition of (R)-1-(3, 5-bis(trifluoromethyl)phenyl)-N-methylethanamine (100 mg, 0.44 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The reaction was stirred at 50° C. for 48 h and quenched with water. The resulting mixture was extracted with EtOAc. The organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the compound 18 (34 mg, yield: 20%) and compound 19 (74 mg, yield: 37%);
Compound 18. 1H NMR (400 MHz, CDCl3) δ 7.68 (s, 1H), 7.45 (s, 2H), 7.05 (d, J=8.5 Hz, 1H), 6.61 (dd, J=21.6, 5.5 Hz, 2H), 5.50-5.43 (m, 1H), 4.04 (d, J=12.9 Hz, 1H), 3.89 (d, J=9.9 Hz, 1H), 3.69 (s, 3H), 3.62 (d, J=8.7 Hz, 1H), 3.15 (d, J=11.7 Hz, 1H), 2.98 (d, J=12.2 Hz, 1H), 2.86 (dd, J=11.8, 9.1 Hz, 1H), 2.59 (s, 3H), 2.41 (s, 3H), 2.28 (ddd, J=31.9, 17.1, 10.8 Hz, 2H), 1.83-1.71 (m, 1H), 1.33 (d, J=7.0 Hz, 3H); m/z (ES+): 558 [M+H]+.
Compound 19: 1H NMR (400 MHz, CDCl3) δ 7.62 (s, 1H), 7.29 (s, 2H), 7.05 (d, J=8.4 Hz, 1H), 6.64-6.57 (m, 2H), 5.51 (d, J=7.4 Hz, 1H), 4.06 (d, J=13.2 Hz, 1H), 3.89 (d, J=9.9 Hz, 1H), 3.69 (s, 3H), 3.56 (d, J=10.1 Hz, 1H), 3.22 (d, J=12.0 Hz, 1H), 3.00 (d, J=12.7 Hz, 1H), 2.81 (dd, J=11.9, 8.6 Hz, 1H), 2.72 (s, 3H), 2.40 (s, 3H), 2.27 (dd, J=19.9, 6.0 Hz, 2H), 1.94-1.86 (m, 1H), 1.76 (s, 1H), 1.42 (d, J=7.2 Hz, 3H); m/z (ES+): 558 [M+H]+.
To a solution of triphosgene (36 mg, 0.1 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 25 (60 mg, 0.24 mmol), DMAP (3 mg, 0.02 mmol) and TEA (73 mg, 0.72 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (78 mg, 0.28 mmol) and TEA (73 mg, 0.72 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (30 mg, yield: 23%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.54 (s, 2H), 7.24-7.17 (m, 1H), 6.90 (d, J=9.9 Hz, 1H), 6.83 (s, 1H), 5.53 (d, J=7.0 Hz, 1H), 4.15 (d, J=12.6 Hz, 1H), 4.01 (d, J=9.8 Hz, 1H), 3.71 (s, 1H), 3.25 (d, J=11.4 Hz, 1H), 3.10 (s, 1H), 2.96 (d, J=12.1 Hz, 1H), 2.70 (s, 3H), 2.52 (s, 3H), 2.46-2.29 (m, 2H), 1.86 (s, 1H), 1.60 (s, 1H), 1.42 (d, J=7.1 Hz, 3H); m/z (ES+): 546 [M+H]+.
To a solution of triphosgene (23 mg, 0.08 mmol) in EtOAc (2 mL) at 0° C. was added a solution of Intermediate 25 (40 mg, 0.161 mmol), DMAP (2 mg, 0.016 mmol) and TEA (49 mg, 0.483 mmol) in EtOAc (2 mL). The mixture was stirred for 2 h, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl)phenyl)ethanamine (44 mg, 0.24 mmol) in EtOAc (2 mL) and TEA (49 mg, 0.483 mmol). The reaction was stirred at 50° C. for 48 h and quenched with water. The resulting mixture was extracted with EtOAc (2×20 mL). The organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=100/1 to 75/1) to give the title compound (10 mg, yield: 13%). 1H NMR (400 MHz, CDCl3) δ 7.32 (s, 1H), 7.20 (dd, J=14.3, 7.7 Hz, 2H), 6.99 (s, 1H), 6.90 (d, J=8.4 Hz, 1H), 6.81 (t, J=7.5 Hz, 1H), 5.47 (d, J=6.7 Hz, 1H), 4.14 (d, J=12.8 Hz, 1H), 4.02 (d, J=9.9 Hz, 1H), 3.67 (d, J=8.2 Hz, 1H), 3.27 (d, J=11.7 Hz, 1H), 3.09 (t, J=12.0 Hz, 1H), 2.93 (t, J=10.8 Hz, 1H), 2.64 (s, 3H), 2.54 (s, 3H), 2.40 (dd, J=10.3, 3.9 Hz, 1H), 2.34 (s, 3H), 2.29 (s, 1H), 1.92-1.77 (m, 1H), 1.66 (d, J=17.5 Hz, 1H), 1.39 (d, J=5.9 Hz, 3H); m/z (ES+): 492 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 25 (162 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3-Isopropyl-5-trifluoromethyl-phenyl)-ethyl]-methyl-amine (163.2 mg, 0.6 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=100/1 to 75/1) to give the title compound (120 mg, yield: 39%). 1H NMR (400 MHz, CDCl3) δ 7.36 (s, 1H), 7.24-7.17 (m, 2H), 7.10 (s, 1H), 6.90 (dd, J=9.8, 2.4 Hz, 1H), 6.83 (dd, J=11.3, 5.3 Hz, 1H), 5.48 (q, J=7.0 Hz, 1H), 4.07 (dd, J=45.2, 11.3 Hz, 2H), 3.67 (dd, J=16.8, 7.6 Hz, 1H), 3.25 (d, J=11.8 Hz, 1H), 3.09 (t, J=12.4 Hz, 1H), 2.91 (ddd, J=20.5, 12.9, 4.9 Hz, 2H), 2.65 (s, 3H), 2.54 (s, 3H), 2.47-2.28 (m, 2H), 1.84 (dt, J=16.7, 6.5 Hz, 1H), 1.69-1.64 (m, 1H), 1.37 (d, J=6.7 Hz, 3H), 1.22 (t, J=6.8 Hz, 6H); m/z (ES+): 520 [M+H]+.
To a solution of triphosgene (29.6 mg, 0.1 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 25 (49.6 mg, 0.2 mmol), DMAP (2.4 mg, 0.02 mmol) and TEA (61 mg, 0.6 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methyl ethanamine (55.5 mg, 0.24 mmol) and TEA (61 mg, 0.6 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (10 mg, yield: 10%); 1H-NMR (400 MHz, CDCl3) δ ppm 7.34 (s, 1H), 7.04-6.91 (m, 2H), 6.90-6.81 (m, 3H), 5.49-5.47 (q, 1H), 4.15-4.00 (dd, 2H), 3.68 (m, 1H), 3.27-2.92 (m, 3H), 2.54 (m, 5H), 2.39 (s, 3H), 2.33 (m, 2H), 2.4 (s, 3H), 1.98 (m, 1H), 1.7 (m, 1H), 1.40 (d, 3H), 1.24 (d, 3H); m/z (ES+): 506 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 27 (120 mg, 0.193 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction mixture was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (74 mg, 1.93 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (60 mg, yield: 50%). 1H NMR (400 MHz, CDCl3) δ 7.34 (s, 1H), 7.26-7.15 (m, 2H), 7.04 (s, 1H), 6.91-6.78 (m, 2H), 5.48 (q, J=6.7 Hz, 1H), 4.05 (dd, J=31.0, 11.3 Hz, 2H), 3.77 (dtd, J=23.6, 11.7, 5.9 Hz, 4H), 3.63-3.54 (m, 1H), 3.28 (d, J=11.8 Hz, 1H), 3.16-3.07 (m, 1H), 2.97 (dt, J=11.8, 5.8 Hz, 1H), 2.69-2.60 (m, 5H), 2.54 (s, 3H), 1.89 (t, J=5.9 Hz, 2H), 1.86-1.79 (m, 3H), 1.72 (dd, J=13.1, 5.6 Hz, 2H), 1.68 (d, J=7.7 Hz, 1H), 1.40 (t, J=11.2 Hz, 3H), 1.21 (t, J=7.6 Hz, 3H); m/z (ES+): 594 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 28 (110 mg, 0.193 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (74 mg, 1.93 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (60 mg, yield: 50%). 1H NMR (400 MHz, CDCl3) δ 7.36 (s, 1H), 7.25 (dd, J=11.2, 5.3 Hz, 1H), 7.17 (s, 1H), 7.09 (s, 1H), 6.89 (dd, J=9.8, 2.5 Hz, 1H), 6.86-6.80 (m, 1H), 5.48 (q, J=6.8 Hz, 1H), 4.08 (d, J=12.7 Hz, 1H), 4.01 (d, J=9.8 Hz, 1H), 3.82 (dt, J=9.9, 5.1 Hz, 1H), 3.74 (ddd, J=17.0, 12.0, 6.4 Hz, 3H), 3.63-3.55 (m, 1H), 3.27 (d, J=11.6 Hz, 1H), 3.10 (td, J=12.5, 3.3 Hz, 1H), 3.03-2.85 (m, 2H), 2.64 (s, 3H), 2.54 (s, 3H), 1.85 (ddd, J=21.0, 11.4, 5.5 Hz, 5H), 1.75-1.66 (m, 2H), 1.37 (d, J=6.4 Hz, 3H), 1.26-1.17 (m, 7H); m/z (ES+): 608 [M+H]+.
To a solution of triphosgene (44 mg, 0.15 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 40 (100 mg, 0.3 mmol), DMAP (4 mg, 0.05 mmol) and TEA (91 mg, 0.9 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (122 mg, 0.45 mmol) in EtOAc (2 mL) and TEA (91 mg, 0.9 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (DCM/MeOH=20/1 to 10/1) to give the title compound (90 mg, yield: 47%); m/z (ES+): 634 [M+H]+.
To a solution of compound 26 (90 mg, 0.142 mmol) in MeOH (20 mL) was added Pd/C (5%, 100 mg). The reaction was stirred under H2 (4 atm) for 12 h. The catalyst was filtered through a celite pad and the solvent was removed in vacuo. The residue was purified by silica gel chromatography (DCM/MeOH=50/1 to 20/1) to give the title compound (20 mg, yield: 26%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.54 (s, 2H), 7.09 (d, J=8.4 Hz, 1H), 6.63 (d, J=2.4 Hz, 1H), 6.55 (d, J=8.3 Hz, 1H), 6.01 (s, 1H), 5.56 (q, J=6.9 Hz, 1H), 4.14 (d, J=12.7 Hz, 1H), 3.98 (d, J=9.9 Hz, 1H), 3.75 (dd, J=16.6, 7.5 Hz, 1H), 3.26 (d, J=11.9 Hz, 1H), 3.17-2.88 (m, 2H), 2.70 (s, 3H), 2.43 (d, J=7.6 Hz, 3H), 2.35 (ddd, J=29.1, 15.3, 9.0 Hz, 2H), 1.89 (ddd, J=16.8, 13.0, 4.0 Hz, 1H), 1.66-1.54 (m, 1H), 1.43 (d, J=6.9 Hz, 3H); m/z (ES+): 544 [M+H]+.
To a solution of Intermediate 41 (50 mg, 0.073 mol) in MeOH (5 mL) was added Pd/C (5%, 30 mg). The reaction was stirred under H2 (4 atm) for 12 h. The catalyst was filtered through a celite pad and the solvent was removed in vacuo. The residue was purified by pre_HPLC to give the title compound (15 mg, yield: 30%); 1H NMR (400 MHz, CDCl3) δ 7.34 (s, 1H), 7.19 (s, 1H), 7.10 (d, J=8.4 Hz, 1H), 7.04 (s, 1H), 6.60 (d, J=2.4 Hz, 1H), 6.51 (d, J=7.6 Hz, 1H), 6.15 (s, 1H), 5.50 (q, J=6.8 Hz, 1H), 4.11 (d, J=12.7 Hz, 1H), 3.98 (d, J=9.9 Hz, 1H), 3.75 (d, J=8.3 Hz, 1H), 3.28 (d, J=11.7 Hz, 1H), 3.10 (t, J=12.2 Hz, 1H), 2.97 (dd, J=11.7, 9.1 Hz, 1H), 2.64 (d, J=4.8 Hz, 4H), 2.46-2.42 (m, 3H), 2.37 (dd, J=14.1, 5.9 Hz, 1H), 1.92-1.85 (m, 1H), 1.70-1.61 (m, 3H), 1.40 (d, J=6.8 Hz, 3H), 1.21 (t, J=7.6 Hz, 3H); m/z (ES+): 504 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 35 (200 mg, 0.32 mmol) in DCM (20 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (120 mg, 3.2 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by prep-HPLC to give the title compound (15 mg, 7%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.54 (s, 2H), 7.27 (s, 1H), 6.82-6.61 (m, 2H), 5.56 (q, J=6.8 Hz, 1H), 4.10 (d, J=12.7 Hz, 1H), 3.97 (d, J=9.8 Hz, 1H), 3.88-3.76 (m, 6H), 3.72-3.51 (m, 2H), 3.27 (d, J=11.6 Hz, 2H), 3.17-2.90 (m, 2H), 2.75-2.57 (m, 3H), 2.50 (s, 3H), 1.94-1.78 (m, 4H), 1.69 (dd, J=14.1, 10.8 Hz, 6H), 1.42 (d, J=6.6 Hz, 4H); m/z (ES+): 646 [M+H]+.
To a solution of intermediate 45 (40 mg, 0.055 mmol) in MeOH (20 mL) was added Pd/C 5% (100 mg). The reaction was stirred under H2 (4 atm) for 12 h. The catalyst was filtered through a celite pad and the solvent was removed in vacuo. The residue was purified by silica gel chromatography (DCM/MeOH=50/1 to 20/1) to give the title compound (20 mg, yield: 58%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.54 (s, 2H), 7.10 (d, J=8.4 Hz, 1H), 6.63 (d, J=2.5 Hz, 1H), 6.56 (d, J=8.4 Hz, 1H), 5.64-5.49 (m, 1H), 5.42 (s, 1H), 4.10 (d, J=12.6 Hz, 1H), 3.96 (d, J=9.8 Hz, 1H), 3.81 (td, J=11.6, 5.5 Hz, 3H), 3.76-3.52 (m, 2H), 3.28 (d, J=11.6 Hz, 1H), 3.19-3.06 (m, 2H), 3.00 (t, J=10.7 Hz, 1H), 2.69 (s, 3H), 2.45 (s, 3H), 2.25 (s, 1H), 1.89 (t, J=5.8 Hz, 3H), 1.86-1.63 (m, 4H), 1.45-1.40 (m, 3H); m/z (ES+): 632 [M+H]+.
To a solution of Intermediate 47 (50 mg, 0.073 mol) in MeOH (20 mL) under N2 atmosphere, was added Pd/C (30 mg). The reaction was stirred under H2 (4 atm) for 12 h. The catalyst was filtered through a celite pad and the solvent was removed in vacuo. The residue was purified by pre_HPLC to give the title compound (15 mg, yield: 30%); 1H-NMR (400 MHz, CDCl3) δ ppm 7.34 (s, 1H), 7.18-7.04 (m, 3H), 6.62-6.54 (m, 2H), 5.51 (q, 1H), 5.36 (s, 1H), 4.10-4.07 (d, 1H), 3.97-3.95 (d, 1H), 3.78-3.6 (m, 5H), 3.26-−2.9 (m, 3H), 2.64 (m, 5H), 2.46 (s, 3H), 2.1 (m, 1H), 1.9-1.6 (m, 6H), 1.37 (d, 3H), 1.2 (d, 3H); m/z (ES+): 592 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of (1S,8aS)-1-o-tolylhexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(4-ethyl-6-(trifluoromethyl)pyridin-2-yl)-N-methylethanamine (163.2 mg, 0.6 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (70 mg, yield: 31%); 1H NMR (400 MHz, CDCl3) δ 7.31 (s, 1H), 7.14 (d, J=11.3 Hz, 2H), 6.68 (s, 1H), 5.53 (s, 1H), 4.14 (d, J=12.4 Hz, 1H), 4.06 (d, J=9.9 Hz, 1H), 3.71 (s, 1H), 3.45 (s, 1H), 3.11 (s, 1H), 2.90 (s, 1H), 2.83 (s, 2H), 2.53 (s, 3H), 2.38 (m, 2H), 1.83 (s, 1H), 1.51 (d, J=6.3 Hz, 3H), 1.15 (t, J=7.1 Hz, 3H); m/z (ES+): 489 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 60 (90 mg, 0.167 mmol) in DCM (10 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (127 mg, 3.34 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by prep-HPLC to give the title compound (20 mg, yield: 21.8%). 1H NMR (400 MHz, CDCl3) δ 7.33 (d, J=9.1 Hz, 2H), 7.24-7.12 (m, 3H), 6.59 (s, 1H), 4.68 (d, J=16.1 Hz, 1H), 4.44 (d, J=16.0 Hz, 1H), 4.11 (dd, J=23.5, 11.4 Hz, 2H), 3.90-3.66 (m, 4H), 3.65-3.50 (m, 2H), 3.33-3.12 (m, 2H), 3.10-2.94 (m, 4H), 2.65-2.38 (m, 4H), 2.27 (s, 3H), 1.91 (t, J=5.9 Hz, 2H), 1.84 (dd, J=12.7, 5.7 Hz, 2H), 1.77-1.74 (m, 2H); m/z (ES+): 549 [M+H]+.
A slow stream of O3 in O2 was passed through a −78° C. cooled solution of intermediate 67 (80 mg, 0.13 mmol) in DCM (10 mL) until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (0.6 g, 16 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the title compound (15 mg, 19%). 1H NMR (400 MHz, CDCl3) δ 7.50 (s, 1H), 7.24 (s, 1H), 7.19 (s, 1H), 7.11 (d, J=7.9 Hz, 1H), 7.00 (s, 1H), 6.94 (d, J=7.8 Hz, 1H), 5.51 (q, J=6.7 Hz, 1H), 4.09 (d, J=12.7 Hz, 1H), 3.99 (s, 1H), 3.91-3.62 (m, 4H), 3.64-3.50 (m, 1H), 3.30 (d, J=11.9 Hz, 1H), 3.12 (td, J=12.4, 3.3 Hz, 1H), 2.99 (td, J=11.9, 2.9 Hz, 1H), 2.64 (s, 3H), 2.60-2.55 (m, 1H), 2.49 (s, 4H), 2.31 (d, J=18.3 Hz, 3H), 1.90-1.74 (m, 5H), 1.76-1.57 (m, 3H), 1.47-1.32 (m, 4H), 1.25 (s, 1H); m/z (ES+): [M+H]+597.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 68 (120 mg, 0.2 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (72 mg, 2 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue The residue was purified by Prep-HPLC to give the compound 1 (20 mg, yield: 17%). 1H-NMR (400 MHz, CDCl3) δ ppm 7.35 (s, 1H), 7.17-6.92 (m, 5H), 5.50-5.49 (q, 1H), 4.09-4.06 (d, 1H), 4.02-3.99 (d, 1H), 3.82-3.70 (m, 5H), 3.59-2.87 (m, 4H), 2.63 (s, 3H), 2.50 (s, 3H), 2.28 (s, 3H), 1.89-1.6 (m, 6H), 1.38-1.3 (m, 9H). m/z (ES+): 604 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 69 (45 mg, 0.07 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (72 mg, 2 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the compound (15 mg, yield: 34%). 1H-NMR (400 MHz, CDCl3) δ ppm 7.75 (s, 1H), 7.46 (s, 2H), 7.28-7.24 (m, 1H), 7.20-7.18 (m, 1H), 6.95-6.90 (m, 2H), 5.61-5.57 (q, 1H), 4.47-4.44 (d, J=10 Hz, 1H), 4.15-4.11 (m, 1H), 3.90 (s, 3H), 3.88-3.80 (m, 3H), 3.78-3.60 (m, 2H), 3.41-3.37 (m, 1H), 3.20-3.13 (m, 1H), 3.04-2.97 (m, 2H), 2.84 (s, 3H), 2.32 (s, 1H), 1.99-1.92 (m, 3H), 1.85-1.78 (m, 3H), 1.50 (d, 2H); m/z (ES+): 632 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 76 (120 mg, 0.193 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (74 mg, 1.93 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the compound (60 mg, yield: 50%). 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.53 (s, 2H), 7.05 (s, 2H), 6.98 (s, 1H), 5.54 (d, J=5.6 Hz, 1H), 4.06 (dd, J=27.0, 10.7 Hz, 2H), 3.76 (d, J=28.8 Hz, 4H), 3.60 (s, 1H), 3.26 (d, J=9.3 Hz, 1H), 3.12 (d, J=11.3 Hz, 1H), 3.01 (d, J=10.4 Hz, 1H), 2.72 (s, 3H), 2.49 (s, 1H), 2.47 (s, 3H), 2.22 (s, 3H), 1.98-1.78 (m, 5H), 1.73 (s, 2H), 1.36 (d, J=4.9 Hz, 3H); m/z (ES+): 630 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 77 (100 mg, 0.17 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (64.31 mg, 1.7 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM, washed with brine, dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified by Prep-HPLC to give the compound (20 mg, yield: 20%). 1H-NMR (400 MHz, CDCl3) δ ppm 7.35 (s, 1H), 7.26 (s, 1H), 7.17-6.97 (m, 4H), 5.50-5.48 (q, 1H), 4.11-4.08 (q, 2H), 3.85-3.70 (m, 4H), 3.61 (m, 1H), 3.12-2.97 (m, 4H), 2.68 (s, 3H), 2.48 (s, 3H), 2.23 (s, 3H), 1.9-1.6 (m, 6H), 1.5-1.40 (m, 9H); m/z (ES+): 604 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of Intermediate 84 (120 mg, 0.193 mmol) in DCM (10 mL) at −78° C. until a pale blue colour persisted. The reaction was purged with N2 to remove the excess of O3 followed by addition of NaBH4 (74 mg, 1.93 mmol). The reaction mixture was allowed to warm to room temperature and stirred for 20 h. After quenched with NH4Cl solution, the resulting mixture was extracted with DCM (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4, and concentrated in vacuo. The residue was purified by Prep-HPLC to give the compound (60 mg, yield: 50%). 1H NMR (400 MHz, CDCl3) δ ppm 7.77 (s, 1H), 7.53 (s, 2H), 7.10-7.04 (m, 3H), 5.56-5.54 (q, 1H), 4.13 (t, 3H), 3.82-3.78 (m, 4H), 3.77 (m, 1H), 3.28 (d, 1H), 3.13-3.10 (m, 1H), 2.7 (s, 3H), 2.4 (s, 3H), 2.31 (s, 3H), 1.9-1.6 (m, 6H), 1.40 (d, 3H); m/z (ES+): 630 [M+H]+.
To a solution of triphosgene (30 mg, 0.102 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 85 (50 mg, 0.204 mmol), DMAP (3 mg, 0.022 mmol) and TEA (62 mg, 0.612 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (83 mg, 0.306 mmol) in EtOAc (2 mL) and TEA (62 mg, 0.612 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (10 mg, yield: 9%). 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.54 (s, 2H), 7.11 (d, J=7.4 Hz, 1H), 7.04 (dd, J=16.2, 7.2 Hz, 2H), 5.55 (d, J=6.8 Hz, 1H), 4.15 (t, J=10.8 Hz, 2H), 3.72 (d, J=8.6 Hz, 1H), 3.27 (d, J=11.2 Hz, 1H), 3.11 (t, J=12.4 Hz, 1H), 2.98 (t, J=10.4 Hz, 1H), 2.70 (s, 3H), 2.41 (s, 3H), 2.33 (d, J=7.0 Hz, 2H), 2.31 (s, 3H), 1.82 (s, 1H), 1.65 (s, 1H), 1.42 (d, J=7.0 Hz, 3H); m/z (ES+): 542 [M+H]+.
To a solution of triphosgene (30 mg, 0.102 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 85 (50 mg, 0.204 mmol), DMAP (3 mg, 0.022 mmol) and TEA (62 mg, 0.612 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (83 mg, 0.306 mmol) and TEA (62 mg, 0.612 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (10 mg, yield: 10%). 1H NMR (400 MHz, CDCl3) δ 7.33 (s, 1H), 7.20 (s, 1H), 7.13 (d, J=7.4 Hz, 1H), 7.03 (d, J=14.4 Hz, 3H), 5.49 (d, J=6.8 Hz, 1H), 4.15 (dd, J=20.9, 11.3 Hz, 2H), 3.69 (d, J=7.7 Hz, 1H), 3.28 (d, J=11.5 Hz, 1H), 3.09 (d, J=12.1 Hz, 1H), 2.98 (d, J=11.3 Hz, 1H), 2.65 (s, 3H), 2.61 (d, J=7.3 Hz, 2H), 2.42 (s, 3H), 2.41-2.33 (m, 2H), 2.32 (s, 3H), 1.80 (s, 1H), 1.64 (s, 1H), 1.37 (d, J=6.3 Hz, 3H), 1.20 (t, J=7.6 Hz, 3H); m/z (ES+): 502 [M+H]+
To a solution of triphosgene (30 mg, 0.102 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 85 (50 mg, 0.204 mmol), DMAP (3 mg, 0.022 mmol) and TEA (62 mg, 0.612 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (83 mg, 0.306 mmol) and TEA (62 mg, 0.612 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (10 mg, yield: 9%). 1H NMR (400 MHz, CDCl3) δ 7.36 (s, 1H), 7.20 (s, 1H), 7.17-7.09 (m, 2H), 7.03 (d, J=8.2 Hz, 2H), 5.49 (d, J=7.0 Hz, 1H), 4.15 (dd, J=19.5, 11.4 Hz, 2H), 3.70 (s, 1H), 3.27 (d, J=11.7 Hz, 1H), 3.08 (d, J=12.4 Hz, 1H), 3.00-2.84 (m, 2H), 2.66 (s, 3H), 2.42 (s, 3H), 2.33 (s, 2H), 2.32 (s, 3H), 1.80 (s, 1H), 1.62 (s, 1H), 1.36 (d, J=7.0 Hz, 3H), 1.22 (t, J=7.0 Hz, 6H); m/z (ES+): 516 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of [1-(3,5-Bis-trifluoromethyl-phenyl)-ethyl]-methyl-amine (163.2 mg, 0.6 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of Intermediate 82 (115.5 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound 43 (120 mg, yield: 39%) and compound 4 4 (25 mg, yield: 8%).
compound 43: 1H NMR (400 MHz, CDCl3) δ 8.36 (d, J=4.0 Hz, 1H), 7.77 (s, 1H), 7.54-7.48 (m, 3H), 7.11 (m, 1H), 5.49 (q, 1H), 4.24-4.09 (m, 3H), 3.48 (m, 1H), 3.24-3.05 (m, 2H), 2.60 (s, 1H), 2.55 (s, 1H), 2.46-2.31 (m, 2H), 1.87-1.80 (m, 1H), 1.50 (d, J=6.4 Hz, 4H); m/z (ES+): 529 [M+H]+.
compound 44: 1H NMR (400 MHz, CDCl3) δ 8.37 (d, J=4.0 Hz, 1H), 7.87 (s, 2H), 7.79 (s, 1H), 7.45 (d, J=7.3 Hz, 1H), 7.10 (dd, J=7.5, 4.8 Hz, 1H), 5.54-5.39 (m, 2H), 4.37-4.30 (m, 1H), 4.18 (d, J=10.6 Hz, 1H), 3.53 (dd, J=12.8, 9.4 Hz, 1H), 3.24 (d, J=13.1 Hz, 1H), 2.98 (dd, J=12.8, 8.7 Hz, 1H), 2.50 (s, 3H), 2.35 (t, J=8.2 Hz, 2H), 2.26 (s, 3H), 2.18-2.04 (m, 1H), 1.61 (d, J=7.1 Hz, 4H); m/z (ES+): 529 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 91 (115.5 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3,5-Bis-trifluoromethyl-phenyl)-ethyl]-methyl-amine (163.2 mg, 0.6 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (120 mg, yield: 39%); m/z (ES+): 529 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 8.63 (d, J=12.6 Hz, 1H), 8.43 (dd, J=16.9, 5.2 Hz, 1H), 7.76 (d, J=29.0 Hz, 1H), 7.56 (s, 1H), 7.46 (d, J=5.0 Hz, 1H), 7.40 (s, 1H), 5.46 (dd, J=28.4, 7.0 Hz, 1H), 4.15 (dd, J=16.8, 11.2 Hz, 2H), 3.77 (s, 1H), 3.32 (d, J=12.2 Hz, 1H), 3.20-3.08 (m, 1H), 2.92 (t, J=11.8 Hz, 1H), 2.81-2.66 (m, 6H), 2.51-2.37 (m, 2H), 1.56 (t, J=14.4 Hz, 2H), 1.43-1.37 (m, 3H), 1.25 (s, 1H). m/z (ES+): 529 [M+H]+.
To a solution of triphosgene (58 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 92 (126.8 mg, 0.4 mmol), DMAP (4.7 mg, 0.04 mmol) and TEA (118 mg, 1.2 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (1S,8aS)-1-(3-methylpyridin-4-yl)-hexahydropyrrolo[1,2-a]pyrazin-6(7H)-one (90 mg, 0.4 mmol) in EtOAc (2 mL) and TEA (118 mg, 1.2 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (10 mg, yield: 10%); 1H NMR (400 MHz, CDCl3) δ 8.45 (d, J=13.8 Hz, 2H), 7.80 (s, 1H), 7.57 (s, 2H), 7.22 (s, 1H), 5.56-5.41 (m, 1H), 4.18 (d, J=13.1 Hz, 1H), 4.06 (d, J=9.7 Hz, 1H), 3.65 (d, J=9.3 Hz, 1H), 3.29 (d, J=11.7 Hz, 1H), 3.11 (s, 1H), 3.00-2.86 (m, 1H), 2.74 (s, 3H), 2.55 (s, 3H), 2.48-2.30 (m, 2H), 1.84 (s, 1H), 1.67 (d, J=8.3 Hz, 1H), 1.42 (d, J=6.9 Hz, 3H); m/z (ES+): 529 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 93 (115 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3,5-Bis-trifluoromethyl-phenyl)-ethyl]-methyl-amine (139.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (20 mg, yield: 10%); 1H NMR (400 MHz, CDCl3) δ 8.44 (d, J=4.5 Hz, 1H), 7.75 (d, J=24.0 Hz, 1H), 7.60-7.51 (m, 3H), 7.10 (dd, J=7.7, 4.5 Hz, 1H), 5.52 (dt, J=20.8, 6.9 Hz, 1H), 4.26-3.95 (m, 2H), 3.70 (dd, J=32.6, 8.0 Hz, 1H), 3.33 (dd, J=28.1, 12.4 Hz, 1H), 3.09 (t, J=11.3 Hz, 1H), 2.98 (dd, J=13.2, 10.3 Hz, 1H), 2.76 (t, J=20.4 Hz, 6H), 2.51-2.35 (m, 2H), 1.52 (d, J=7.2 Hz, 1H), 1.43 (d, J=6.8 Hz, 3H); m/z (ES+): 529 [M+H]+.
Triphosgene (34.9 mg, 0.119 mmol) was dissolved EtOAc (2 mL), a solution of Intermediate 98 (70 mg, 0.297 mmol), DMAP (4 mg) and TEA (0.13 ml, 0.89 mmol) in EtOAc (2 mL) was added at 0′C. The mixture was stirring for 1.5 h, then a solution (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (112.5 mg, 0.415 mmol) and TEA (0.13 ml, 0.89 mmol) in EtOAc (2 mL) was added. The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (20 mg, Yield: 13%). 1H NMR (400 MHz, CDCl3) δ ppm 7.78 (s, 1H), 7.60 (s, 2H), 6.86 (s, 1H), 6.79 (s, 1H), 5.57-5.62 (m, 1H), 4.04-4.14 (m, 2H), 3.66-3.70 (m, 1H), 2.91-3.11 (m, 3H), 2.74 (s, 3H), 2.34-2.45 (m, 2H), 2.22 (s, 3H), 1.99-2.04 (m, 1H), 1.74-1.79 (m, 1H), 1.74 (d, J=3.2 Hz, 3H); m/z (ES+): 534 [M+H]+.
To a solution of triphosgene (63 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 98 (100 mg, 0.42 mmol), DMAP (5 mg, 0.04 mmol) and TEA (127 mg, 1.26 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (116 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (127 mg, 1.26 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (30 mg, yield: 14%); 1H NMR (400 MHz, CDCl3) δ 7.27 (s, 1H), 7.16 (s, 1H), 7.06 (s, 1H), 6.79 (s, 1H), 6.72 (s, 1H), 5.47 (d, J=7.0 Hz, 1H), 4.06-3.96 (m, 2H), 3.62-3.51 (m, 1H), 3.15-3.06 (m, 1H), 3.04-2.93 (m, 1H), 2.86 (dd, J=11.7, 3.3 Hz, 1H), 2.63 (s, 3H), 2.61-2.54 (m, 2H), 2.44-2.23 (m, 2H), 2.15 (d, J=0.6 Hz, 3H), 1.99-1.86 (m, 1H), 1.75-1.64 (m, 1H), 1.35 (d, J=7.0 Hz, 3H), 1.16 (dd, J=13.5, 5.9 Hz, 3H); m/z (ES+): 494 [M+H]+.
(1R,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-1-(3-ethylthiophen-2-yl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide
To a solution of triphosgene (63 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 99 (100 mg, 0.42 mmol), DMAP (5 mg, 0.04 mmol) and TEA (127 mg, 1.26 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (135 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (127 mg, 1.26 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (30 mg, yield: 13%); 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.59 (s, 2H), 7.13 (d, J=5.1 Hz, 1H), 6.79 (d, J=5.1 Hz, 1H), 5.58 (q, J=6.9 Hz, 1H), 4.17-4.06 (m, 2H), 3.69 (dd, J=16.9, 7.3 Hz, 1H), 3.19 (d, J=11.9 Hz, 1H), 3.08 (t, J=12.3 Hz, 1H), 2.93 (td, J=11.7, 3.2 Hz, 1H), 2.72 (s, 3H), 2.50-2.35 (m, 2H), 2.33 (d, J=12.2 Hz, 3H), 1.96 (dd, J=10.3, 6.5 Hz, 1H), 1.76-1.66 (m, 1H), 1.46 (d, J=7.0 Hz, 3H); m/z (ES+): 534 [M+H]+.
To a solution of triphosgene (63 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 99 (100 mg, 0.42 mmol), DMAP (5 mg, 0.04 mmol) and TEA (127 mg, 1.26 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (116 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (127 mg, 1.26 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (30 mg, yield: 15.7%); 1H NMR (400 MHz, CDCl3) δ 7.27 (s, 1H), 7.16 (s, 1H), 7.06 (d, J=4.2 Hz, 2H), 6.72 (d, J=5.1 Hz, 1H), 5.45 (q, J=6.9 Hz, 1H), 4.05 (dd, J=11.2, 6.4 Hz, 2H), 3.61 (dd, J=16.9, 7.4 Hz, 1H), 3.11 (d, J=11.9 Hz, 1H), 3.00 (t, J=12.3 Hz, 1H), 2.83 (td, J=11.7, 3.1 Hz, 1H), 2.61 (s, 3H), 2.60-2.55 (m, 2H), 2.45-2.22 (m, 5H), 1.89 (ddd, J=16.9, 13.1, 3.9 Hz, 1H), 1.73-1.64 (m, 1H), 1.32 (d, J=7.0 Hz, 3H), 1.16 (dd, J=13.9, 6.3 Hz, 4H); m/z (ES+): 494 [M+H]+.
To a solution of triphosgene (63 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 99 (100 mg, 0.42 mmol), DMAP (5 mg, 0.04 mmol) and TEA (127 mg, 1.26 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl) phenyl) ethanamine (115.5 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (127 mg, 1.26 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (30 mg, yield: 16.2%); 1H NMR (400 MHz, CDCl3) δ 7.32 (s, 1H), 7.21 (s, 1H), 7.13 (d, J=5.1 Hz, 1H), 7.10 (s, 1H), 6.80 (d, J=5.1 Hz, 1H), 5.51 (d, J=6.9 Hz, 1H), 4.16-4.08 (m, 2H), 3.68 (d, J=9.7 Hz, 1H), 3.19 (d, J=11.4 Hz, 1H), 3.05 (d, J=12.4 Hz, 1H), 2.92 (dd, J=11.8, 3.2 Hz, 1H), 2.68 (s, 3H), 2.49-2.28 (m, 8H), 2.02-1.90 (m, 1H), 1.69 (d, J=10.4 Hz, 1H), 1.38 (d, J=7.0 Hz, 3H); m/z (ES+): 481 [M+H]+.
To a solution of triphosgene (46.8 mg, 0.15 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 100 (70 mg, 0.31 mmol), DMAP (3.8 mg, 0.03 mmol) and TEA (95.5 mg, 0.94 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (102.5 mg, 0.37 mmol) in EtOAc (2 mL) and TEA (95.5 mg, 0.94 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (20 mg, yield: 12.2%); 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.59 (s, 2H), 7.23 (d, J=5.1 Hz, 1H), 7.06 (d, J=3.3 Hz, 1H), 6.97 (dd, J=5.0, 3.6 Hz, 1H), 5.58 (q, J=6.8 Hz, 1H), 4.12 (dd, J=11.1, 7.5 Hz, 2H), 3.71 (dd, J=17.0, 7.3 Hz, 1H), 3.21 (d, J=11.9 Hz, 1H), 3.09 (t, J=12.3 Hz, 1H), 2.96 (td, J=11.6, 3.2 Hz, 1H), 2.74 (s, 3H), 2.50-2.32 (m, 2H), 2.06-1.94 (m, 1H), 1.83-1.70 (m, 1H), 1.47 (d, J=7.0 Hz, 3H); m/z (ES+): 520 [M+H]+.
To a solution of triphosgene (46.8 mg, 0.15 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 100 (70 mg, 0.31 mmol), DMAP (3.8 mg, 0.03 mmol) and TEA (95.5 mg, 0.94 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (87.3 mg, 0.37 mmol) in EtOAc (2 mL) and TEA (95.5 mg, 0.94 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (20 mg, yield: 13.2%); 1H NMR (400 MHz, CDCl3) δ 7.27 (s, 1H), 7.16 (d, J=5.6 Hz, 2H), 7.04 (s, 1H), 7.00 (d, J=3.0 Hz, 1H), 6.91 (dd, J=4.8, 3.7 Hz, 1H), 5.46 (q, J=6.9 Hz, 1H), 4.05 (d, J=9.9 Hz, 2H), 3.61 (dd, J=16.8, 7.3 Hz, 1H), 3.12 (d, J=11.8 Hz, 1H), 3.00 (t, J=12.2 Hz, 1H), 2.86 (td, J=11.7, 3.2 Hz, 1H), 2.62 (s, 3H), 2.60-2.55 (m, 2H), 2.43-2.24 (m, 2H), 1.91 (ddd, J=17.1, 13.2, 3.9 Hz, 1H), 1.69 (dt, J=13.4, 9.5 Hz, 1H), 1.34 (d, J=7.0 Hz, 3H), 1.15 (t, J=7.6 Hz, 3H); m/z (ES+): 480 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of Intermediate 101 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (70 mg, yield: 31%); 1H NMR (400 MHz, CDCl3) δ 7.32 (s, 1H), 7.22 (s, 1H), 7.09 (s, 1H), 6.83 (d, J=3.3 Hz, 1H), 6.61 (d, J=3.1 Hz, 1H), 5.58-5.49 (m, 1H), 4.10 (d, J=12.7 Hz, 1H), 4.00 (d, J=9.7 Hz, 1H), 3.65 (dd, J=17.0, 7.3 Hz, 1H), 3.19 (d, J=11.9 Hz, 1H), 3.06 (t, J=12.2 Hz, 1H), 2.97-2.85 (m, 1H), 2.68 (s, 3H), 2.49-2.41 (m, 4H), 2.40-2.31 (m, 4H), 2.05-1.91 (m, 1H), 1.82-1.71 (m, 1H), 1.42 (dd, J=17.4, 7.0 Hz, 3H). m/z (ES+):480 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of Intermediate 101 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (70 mg, yield: 31%); 1H NMR (400 MHz, CDCl3) δ 7.34 (s, 1H), 7.24 (s, 1H), 7.14 (s, 1H), 6.84 (t, J=4.9 Hz, 1H), 6.60 (d, J=2.8 Hz, 1H), 5.55 (q, J=6.9 Hz, 1H), 4.09 (d, J=12.7 Hz, 1H), 4.00 (d, J=9.7 Hz, 1H), 3.75-3.56 (m, 1H), 3.18 (d, J=11.9 Hz, 1H), 3.06 (t, J=12.1 Hz, 1H), 2.96-2.83 (m, 1H), 2.72-2.58 (m, 5H), 2.50-2.28 (m, 5H), 2.00 (ddd, J=17.1, 13.1, 4.0 Hz, 1H), 1.83-1.57 (m, 4H), 1.43 (t, J=9.6 Hz, 3H), 1.23 (t, J=7.6 Hz, 4H); m/z (ES+): 494 [M+H]+.
To a solution of triphosgene (29.6 mg, 0.1 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 101 (49.6 mg, 0.2 mmol), DMAP (2.4 mg, 0.02 mmol) and TEA (61 mg, 0.6 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of [1-(3,5-Bis-trifluoromethyl-phenyl)-ethyl]-methyl-amine (54.2 mg, 0.24 mmol) in EtOAc (2 mL) and TEA (61 mg, 0.6 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (10 mg, yield: 10%); 1H NMR (400 MHz, CDCl3) δ 7.79 (s, 1H), 7.61 (s, 2H), 6.82 (d, J=3.4 Hz, 1H), 6.65-6.55 (m, 1H), 5.60 (q, J=7.0 Hz, 1H), 4.12 (td, J=10.4, 5.2 Hz, 1H), 4.00 (d, J=9.7 Hz, 1H), 3.74-3.64 (m, 1H), 3.24-3.16 (m, 1H), 3.06 (dd, J=19.6, 7.4 Hz, 1H), 2.94 (td, J=11.7, 3.3 Hz, 1H), 2.73 (s, 3H), 2.46-2.40 (m, 4H), 2.01 (tdd, J=11.5, 7.6, 3.9 Hz, 1H), 1.77-1.66 (m, 2H), 1.47 (dd, J=25.3, 7.0 Hz, 3H).
To a solution of triphosgene (63 mg, 0.2 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 102 (100 mg, 0.42 mmol), DMAP (5 mg, 0.04 mmol) and TEA (127 mg, 1.26 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (135 mg, 0.5 mmol) in EtOAc (2 mL) and TEA (127 mg, 1.26 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (40 mg, yield: 17.7%); 1H NMR (400 MHz, CDCl3) δ 7.78 (d, J=13.2 Hz, 1H), 7.62 (s, 1H), 7.59-7.52 (m, 2H), 5.68-5.53 (m, 1H), 4.17-4.04 (m, 2H), 3.78-3.53 (m, 1H), 3.26 (dd, J=26.8, 12.2 Hz, 1H), 3.13-2.87 (m, 2H), 2.78 (d, J=35.2 Hz, 3H), 2.67 (d, J=9.5 Hz, 3H), 2.53-2.32 (m, 2H), 2.09-1.94 (m, 1H), 1.81-1.68 (m, 1H), 1.54 (dd, J=15.7, 7.1 Hz, 3H). m/z (ES+): 535 [M+H]+.
(1R,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-6-oxo-1-(thiazol-5-yl)-hexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide
To a solution of triphosgene (33 mg, 0.112 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 103 (50 mg, 0.224 mmol), DMAP (3 mg, 0.022 mmol) and TEA (68 mg, 0.672 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (91 mg, 0.336 mmol) in EtOAc (2 mL) and TEA (68 mg, 0.672 mmol). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The resulting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography (PE/EtOAc=5/1 to 1/1) to give the title compound (10 mg, yield: 9%); m/z (ES+): 521 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 8.69 (d, J=9.8 Hz, 1H), 7.81 (d, J=3.0 Hz, 1H), 7.71 (d, J=15.1 Hz, 1H), 7.53 (s, 1H), 7.42 (s, 1H), 5.50 (dt, J=20.9, 7.1 Hz, 1H), 4.11 (dt, J=22.8, 11.3 Hz, 2H), 3.74-3.46 (m, 1H), 3.19 (dd, J=27.8, 11.5 Hz, 1H), 3.10-2.82 (m, 2H), 2.77 (s, 1H), 2.68 (s, 2H), 2.43-2.25 (m, 2H), 2.02-1.83 (m, 1H), 1.69 (dd, J=19.3, 10.0 Hz, 1H), 1.45 (dd, J=27.3, 6.8 Hz, 3H).
(1S,8aS)—N—((R)-1-(3,5-bis(trifluoromethyl)phenyl)ethyl)-N-methyl-1-(3-methyl-1H-pyrazol-4-yl)-6-oxohexahydropyrrolo[1,2-a]pyrazine-2(1H)-carboxamide
To a solution of Intermediate 110 (60 mg, 0.1 mmol) in MeOH (5 mL) under N2 atmosphere, was added Pd/C (30 mg). The reaction mixture was stirred under H2 atmosphere (4 atm) for 24 h at room temperature. Pd/C was filtered off through a celite pad, and the filtrate was concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (15 mg, yield: 29%); 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.55 (s, 2H), 7.48-7.36 (m, 1H), 5.65-5.52 (m, 1H), 4.13 (d, J=12.9 Hz, 1H), 3.77 (dd, J=17.1, 7.3 Hz, 1H), 3.68 (d, J=10.0 Hz, 1H), 3.24-3.10 (m, 2H), 3.06 (t, J=10.8 Hz, 2H), 2.91 (td, J=11.8, 3.0 Hz, 2H), 2.69 (s, 4H), 2.46-2.32 (m, 6H), 2.03-1.89 (m, 1H), 1.61-1.50 (m, 2H), 1.46 (d, J=7.0 Hz, 3H). m/z (ES+): 518[M+H]+.
To a solution of Intermediate 111 (60 mg, 0.1 mmol) in MeOH (5 mL) under N2 atmosphere, was added Pd/C (30 mg). The reaction mixture was stirred under H2 atmosphere (4 atm) for 24 h at room temperature. Pd/C was filtered off through a celite pad, and the filtrate was concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (22 mg, yield: 46%); 1H NMR (400 MHz, CDCl3) δ 7.79 (d, J=11.6 Hz, 1H), 7.35 (s, 1H), 7.18 (s, 1H), 7.11 (s, 1H), 5.48 (q, J=6.9 Hz, 1H), 4.14 (d, J=12.7 Hz, 1H), 3.86-3.65 (m, 1H), 3.21 (d, J=11.9 Hz, 1H), 3.06 (t, J=12.3 Hz, 1H), 2.87 (dt, J=11.8, 5.9 Hz, 1H), 2.74-2.60 (m, 2H), 2.60-2.46 (m, 1H), 2.48-2.31 (m, 1H), 2.00 (dd, J=11.6, 8.3 Hz, 1H), 1.66-1.50 (m, 1H), 1.46 (dd, J=17.5, 7.1 Hz, 1H), 1.29-1.15 (m, 1H). m/z (ES+): 478[M+H]+.
To a solution of Intermediate 115 (40 mg, 0.057 mol) in MeOH (20 mL) under N2 atmosphere, was added Pd/C (30 mg). The reaction mixture was stirred under H2 atmosphere (4 atm) for 24 h at room temperature. Pd/C was filtered off through a celite pad, and the filtrate was concentrated in high vacuum. The residue was purified by pre_HPLC to give the title compound (15 mg, yield: 30%); 1H NMR (400 MHz, CDCl3) δ 7.41 (s, 1H), 7.34 (s, 1H), 7.18 (s, 1H), 7.08 (s, 1H), 5.53 (d, J=6.9 Hz, 1H), 4.08 (d, J=11.7 Hz, 1H), 3.79 (d, J=9.0 Hz, 3H), 3.71-3.59 (m, 3H), 3.19 (d, J=11.6 Hz, 1H), 3.09 (t, J=12.1 Hz, 1H), 2.90 (d, J=10.5 Hz, 1H), 2.71-2.65 (m, 2H), 2.62 (s, 3H), 2.36 (s, 3H), 1.96 (dd, J=13.1, 6.8 Hz, 1H), 1.87 (d, J=18.3 Hz, 3H), 1.76-1.71 (m, 1H), 1.64-1.56 (m, 1H), 1.41 (d, J=6.8 Hz, 3H), 1.26-1.20 (m, 5H), 0.87 (d, J=7.1 Hz, 1H).
To a solution of Intermediate 117 (40 mg, 0.062 mmol) in MeOH (20 mL) was added Pd/C 5% (100 mg). The reaction was stirred under H2 (5 atm) for 12 h. The catalyst was filtered through a celite pad and the solvent was removed in vacuo. The residue was purified by silica gel chromatography (DCM/MeOH=50/1 to 20/1) to give the title compound (20 mg, yield: 58%). m/z (ES+): 552 [M+H]+. 1H NMR (400 MHz, CDCl3) δ 7.42 (s, 1H), 7.32 (s, 1H), 7.17 (s, 1H), 7.03 (s, 1H), 5.51 (d, J=6.9 Hz, 1H), 4.09 (d, J=12.5 Hz, 1H), 3.86-3.77 (m, 3H), 3.67 (ddd, J=23.1, 11.6, 5.7 Hz, 3H), 3.20 (d, J=12.0 Hz, 1H), 3.08 (t, J=12.2 Hz, 1H), 2.91 (t, J=10.6 Hz, 1H), 2.63 (s, 3H), 2.35 (d, J=12.4 Hz, 6H), 1.92 (dt, J=33.5, 13.5 Hz, 5H), 1.81-1.69 (m, 2H), 1.61 (dd, J=13.3, 7.7 Hz, 2H), 1.41 (d, J=6.9 Hz, 3H).
To a suspension of NaH (60%) (20 mg, 0.15 mmol) in THF (4 mL) was dropwisely added compound 59 (80 mg, 0.15 mmol) in THF (10 mL) at 0° C., After stirred at the same temperature for 30 min, MeI (0.1 ml, 0.15 mmol) was added into the reaction mixture at 0° C. The reaction mixture was stirred at 15° C. for 1 h and carefully poured into ice-water (200 mL) contained con H2SO4 (6 mL). The resulting mixture was extracted with 30 ml EtOAc, washed with brine (10 mL) and the combined organic layers were dried over anhydrous Na2SO4 and then concentrated. The residue was purified by Prep-HPLC to give the title compound (20 mg, yield: 25%). 1H NMR (400 MHz, CDCl3) δ 7.70 (d, J=10.4 Hz, 1H), 7.47 (t, J=17.9 Hz, 2H), 7.25-7.09 (m, 1H), 5.51 (q, J=6.6 Hz, 1H), 4.04 (dd, J=11.1, 9.3 Hz, 1H), 3.79-3.69 (m, 3H), 3.69-3.49 (m, 2H), 3.15 (dd, J=33.3, 21.7 Hz, 1H), 3.09-2.65 (m, 3H), 2.65 (d, J=27.2 Hz, 3H), 2.34 (ddd, J=19.4, 11.8, 6.6 Hz, 2H), 2.25-2.17 (m, 3H), 1.95-1.83 (m, 1H), 1.43 (dd, J=19.7, 12.7 Hz, 3H); m/z (ES+): 532 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of Intermediate 85 115.5 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at room temperature, followed by addition of (R)—N-methyl-1-(3-methyl-5-(trifluoromethyl) phenyl)ethanamine (163.2 mg, 0.6 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The reaction was stirred at 45° C. for 48 h and quenched with sat. NH4Cl (aq) solution. The re suiting mixture was extracted with EtOAc (2×20 mL). The combined organic layers were washed with brine, dried over anhydrous Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the title compound (15 mg, yield: 6%). 1H NMR (400 MHz, CDCl3) δ 7.31 (s, 1H), 7.18 (s, 1H), 7.11 (s, 1H), 7.03 (d, J=17.3 H z, 3H), 5.48 (s, 1H), 4.14 (d, J=21.7 Hz, 2H), 3.70 (s, 1H), 3.28 (s, 1H), 3.11 (s, 1H), 2.97 (s, 1H), 2.64 (s, 3H), 2.42 (s, 3H), 2.35 (s, 2H), 2.32 (d, J=5.1 Hz, 6H), 1.84-1.78 (m, 1H), 1.67-1.63 (m, 1H), 1.57 (s, 3H); m/z (ES+): 488 [M+H]+.
To a solution of triphosgene (244.3 mg, 0.83 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 122 (305 mg, 1.15 mmol), DMAP (17 mg, 0.14 mmol) and TEA (350.5 mg, 3.46 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (377 mg, 1.38 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with EtOAc; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (250 mg, yield: 39%); 1H-NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.54 (s, 1H), 7.34-7.24 (m, 2H), 7.18 (d, J=7.4 Hz, 1H), 7.07 (s, 1H), 5.53 (q, J=7.1 Hz, 1H), 4.14 (dd, J=11.0, 8.4 Hz, 2H), 3.69 (d, J=9.0 Hz, 1H), 3.28 (d, J=11.5 Hz, 1H), 3.08 (dd, J=13.1, 2.2 Hz, 1H), 2.98 (dd, J=12.0, 3.0 Hz, 1H), 2.71 (s, 3H), 2.57 (s, 3H), 2.45-2.27 (m, 2H), 1.84 (dd, J=6.5, 2.8 Hz, 1H), 1.71-1.48 (m, 1H), 1.43 (d, J=6.9 Hz, 3H); m/z (ES+): 562 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 125 (122 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound 68 (40 mg, yield: 15%) and compound 69 (40 mg, yield: 15%).
Compound 68: 1H-NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.53 (s, 1H), 7.36-7.18 (m, 3H), 7.11 (t, J=7.2 Hz, 1H), 5.53 (q, J=6.8 Hz, 1H), 4.13 (t, J=10.8 Hz, 2H), 3.79 (dd, J=16.3, 7.5 Hz, 1H), 3.26 (d, J=11.8 Hz, 1H), 3.11 (t, J=12.2 Hz, 1H), 2.99 (t, J=11.3 Hz, 2H), 2.95-2.76 (m, 1H), 2.7 (s, 3H) 2.51-2.23 (m, 2H), 1.84 (dd, J=12.4, 8.2 Hz, 1H), 1.39 (d, J=6.9 Hz, 3H), 1.33 (t, J=7.5 Hz, 3H); m/z (ES+): 542 [M+H]+.
Compound 69: 1H-NMR (400 MHz, CDCl3) δ 7.71 (s, 1H), 7.36 (s, 2H), 7.23 (dd, J=7.0, 3.2 Hz, 3H), 7.12 (d, J=7.4 Hz, 1H), 5.57 (q, J=7.1 Hz, 1H), 4.14 (t, J=12.7 Hz, 2H), 3.70 (dd, J=16.9, 7.3 Hz, 1H), 3.31 (d, J=12.1 Hz, 1H), 3.19-3.07 (m, 1H), 3.06-2.88 (m, 2H), 2.88-2.76 (m, 4H), 2.48-2.26 (m, 2H), 1.91-1.78 (m, 1H), 1.78-1.67 (m, 1H), 1.50 (d, J=7.1 Hz, 3H), 1.33 (t, J=7.5 Hz, 3H); m/z (ES+): 542 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 125 (122 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methyl ethanamine (138.6 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound 70 (40 mg, yield: 16%) and compound 71 (40 mg, yield: 16%).
Compound 70: 1H-NMR (400 MHz, CDCl3) δ 7.33 (s, 1H), 7.29 (s, 1H), 7.26-7.20 (m, 2H), 7.19 (s, 1H), 7.12 (t, J=7.3 Hz, 1H), 7.04 (s, 1H), 5.48 (q, J=6.9 Hz, 1H), 4.12 (d, J=10.0 Hz, 2H), 3.76 (dd, J=16.8, 7.5 Hz, 1H), 3.27 (d, J=12.1 Hz, 1H), 3.10 (dd, J=20.0, 7.4 Hz, 1H), 3.06-2.92 (m, 2H), 2.86 (dd, J=15.1, 7.5 Hz, 1H), 2.67-2.58 (m, 5H), 2.46-2.26 (m, 2H), 1.83 (ddd, J=13.2, 12.2, 4.0 Hz, 1H), 1.68 (dd, J=11.2, 5.9 Hz, 1H), 1.34 (t, J=7.5 Hz, 6H), 1.20 (t, J=7.6 Hz, 6H); m/z (ES+): 502 [M+H]+.
Compound 71: 1H-NMR (400 MHz, CDCl3) δ 7.29 (d, J=8.8 Hz, 2H), 7.26-7.20 (m, 2H), 7.14 (t, J=7.2 Hz, 1H), 7.05 (s, 1H), 6.78 (s, 1H), 5.52 (q, J=7.0 Hz, 1H), 4.13 (t, J=10.7 Hz, 2H), 3.71 (dd, J=16.8, 7.4 Hz, 1H), 3.29 (d, J=12.0 Hz, 1H), 3.16-3.06 (m, 1H), 3.02 (dd, J=15.1, 7.6 Hz, 1H), 2.92 (td, J=12.0, 3.1 Hz, 2H), 2.75 (s, 3H), 2.52 (q, J=7.6 Hz, 2H), 2.48-2.26 (m, 2H), 1.82 (s, 1H), 1.72 (d, J=7.8 Hz, 1H), 1.44 (d, J=7.1 Hz, 3H), 1.34 (t, J=7.5 Hz, 3H), 1.15 (t, J=7.6 Hz, 3H); m/z (ES+): 502 [M+H]+.
To a solution of triphosgene (73.45 mg, 0.25 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 128 (125 mg, 0.5 mmol), DMAP (6.1 mg, 0.05 mmol) and TEA (151.68 mg, 1.5 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (151.68 mg, 1.5 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with EtOAc; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound 72 (20 mg, yield: 8%) and compound 73 (20 mg, yield: 8%).
Compound 72: 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.55 (s, 2H), 7.42 (dd, J=8.2, 4.6 Hz, 2H), 7.31 (dd, J=5.8, 3.7 Hz, 1H), 7.25-7.19 (m, 2H), 5.51 (q, J=7.0 Hz, 1H), 4.44 (d, J=10.0 Hz, 1H), 4.14 (d, J=12.3 Hz, 1H), 3.76 (d, J=9.5 Hz, 1H), 3.33 (d, J=11.6 Hz, 1H), 3.07 (dd, J=19.7, 13.7 Hz, 2H), 2.72 (s, 3H), 2.52-2.40 (m, 1H), 2.36 (dd, J=17.7, 8.9 Hz, 1H), 1.87 (dd, J=14.1, 6.1 Hz, 2H), 1.45 (d, J=7.0 Hz, 3H); m/z (ES+): 548 [M+H]+.
Compound 73: 1H NMR (400 MHz, CDCl3) δ 7.72 (s, 1H), 7.44-7.38 (m, 3H), 7.29 (d, J=5.2 Hz, 1H), 7.24-7.19 (m, 2H), 5.58 (t, J=7.0 Hz, 1H), 4.45 (d, J=9.9 Hz, 1H), 4.17 (d, J=13.0 Hz, 1H), 3.67 (d, J=9.4 Hz, 1H), 3.39 (d, J=12.1 Hz, 1H), 3.13 (t, J=12.1 Hz, 1H), 3.02-2.91 (m, 1H), 2.82 (s, 3H), 2.54-2.42 (m, 1H), 2.40-2.30 (m, 1H), 1.98 (dd, J=33.8, 9.1 Hz, 2H), 1.85 (dd, J=12.4, 8.8 Hz, 1H), 1.51 (d, J=7.2 Hz, 3H); m/z (ES+): 548 [M+H]+.
A slow stream of O3 in O2 was passed through a cooled solution of intermediate 134 (150 mg, 0.28 mmol) at −78° C. in DCM (30 mL) until a pale blue colour persisted. Excess of O3 was purged by N2 bubbling, then NaBH4 (105 mg, 2.8 mmol) was added. The solution was allowed to reach 25° C. and stirred for 48 h. The solvent was removed in vacuo and the crude material was purified by pre_HPLC to give the title compound as white solid (50 mg, yield: 33%). 1H NMR (400 MHz, CDCl3) δ 7.80 (s), 7.55 (s), 7.38 (s), 7.28 (t, J=3.4 Hz), 5.50 (d, J=6.8 Hz), 5.11 (d, J=11.6 Hz), 4.86 (d, J=8.9 Hz), 4.49 (dd, J=11.4, 9.2 Hz), 4.21 (dd, J=26.2, 11.3 Hz), 3.68 (d, J=9.5 Hz), 3.27 (d, J=11.7 Hz), 3.14 (s), 3.00 (dd, J=11.8, 3.0 Hz), 2.72 (s), 2.52-2.27 (m), 1.86-1.67 (m), 1.40 (d, J=6.9 Hz); m/z (ES+): 544[M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 138 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (70 mg, yield: 31%). 1H NMR (400 MHz, CDCl3) δ 7.78 (s, 1H), 7.59 (s, 2H), 6.78 (s, 1H), 5.59 (q, J=6.9 Hz, 1H), 4.13 (dd, J=11.2, 6.5 Hz, 2H), 3.69 (dd, J=17.0, 7.3 Hz, 1H), 3.19 (d, J=11.8 Hz, 1H), 3.06 (d, J=12.4 Hz, 1H), 2.92 (td, J=11.7, 3.2 Hz, 1H), 2.71 (s, 3H), 2.47-2.27 (m, 2H), 2.22 (s, 3H), 2.14 (s, 3H), 2.06-1.89 (m, 1H), 1.74-1.61 (m, 4H), 1.47 (d, J=7.0 Hz, 3H). m/z (ES+):548 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 138 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (70 mg, yield: 31%). 1H NMR (400 MHz, CDCl3) δ 7.34 (s, 1H), 7.23 (s, 1H), 7.13 (s, 1H), 6.79 (s, 1H), 5.53 (q, J=6.9 Hz, 1H), 4.17-4.07 (m, 2H), 3.68 (dd, J=17.0, 7.3 Hz, 1H), 3.18 (d, J=11.7 Hz, 1H), 3.06 (t, J=12.2 Hz, 1H), 2.90 (td, J=11.7, 3.3 Hz, 1H), 2.68-2.60 (m, 5H), 2.48-2.31 (m, 2H), 2.23 (s, 3H), 2.14 (d, J=0.7 Hz, 3H), 2.05-1.92 (m, 1H), 1.76-1.64 (m, 1H), 1.62 (s, 9H), 1.40 (d, J=7.0 Hz, 3H), 1.23 (dd, J=13.8, 6.2 Hz, 4H); m/z (ES+): 509[M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 143 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (70 mg, yield: 31%). 1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.55 (s, 2H), 7.09 (t, J=8.0 Hz, 1H), 6.86 (d, J=7.7 Hz, 1H), 6.86 (d, J=7.7 Hz, 1H), 6.76 (d, J=8.1 Hz, 1H), 5.56 (q, J=6.9 Hz, 1H), 4.22-4.11 (m, 2H), 3.83 (s, 3H), 3.70 (dd, J=21.9, 14.5 Hz, 1H), 3.28 (d, J=11.7 Hz, 1H), 3.11-3.04 (m, 1H), 2.97 (td, J=11.8, 3.0 Hz, 1H), 2.69-2.65 (m, 1H), 2.47-2.23 (m, 5H), 1.88-1.81 (m, 1H), 1.67 (dd, J=19.6, 10.1 Hz, 3H), 1.44 (d, J=7.0 Hz, 3H), 1.25 (s, 1H); m/z (ES+): 558 [M+H]+.
To a solution of triphosgene (47 mg, 0.16 mmol) in EtOAc (10 mL) at 0° C. was added solution of intermediate 143 (120 mg, 0.35 mmol), DMAP (4 mg, 0.035 mmol) and TEA (107 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl)phenyl)-N-methyl ethanamine (163.2 mg, 0.6 mmol) in EtOAc (2 mL) and TEA (107 mg, 1.05 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (70 mg, yield: 31%). 1H NMR (400 MHz, CDCl3) δ 7.36 (s, 1H), 7.20 (s, 1H), 7.15-7.07 (m, 2H), 6.89 (d, J=7.8 Hz, 1H), 6.75 (d, J=8.1 Hz, 1H), 5.50 (q, J=6.9 Hz, 1H), 4.18-4.05 (m, 2H), 3.82 (s, 3H), 3.68 (dd, J=16.7, 7.5 Hz, 1H), 3.26 (d, J=12.2 Hz, 1H), 3.09 (t, J=12.5 Hz, 1H), 3.00-2.83 (m, 2H), 2.66 (s, 3H), 2.50-2.34 (m, 4H), 2.30 (dd, J=17.2, 8.7 Hz, 1H), 1.83-1.75 (m, 1H), 1.72-1.65 (m, 1H), 1.44-1.35 (m, 3H), 1.33-1.14 (m, 7H); m/z (ES+): 532 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 149 (82 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3, 5-bis(trifluoromethyl)phenyl)-N-methylethanamine (119 mg, 0.44 mmol) in EtOAc (2 mL) and TEA (97 mg, 0.96 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (20 mg, yield: 15%); 1H-NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.55 (s, 2H), 7.14 (d, J=7.1 Hz, 1H), 7.04 (dd, J=16.2, 7.3 Hz, 2H), 5.54 (q, J=7.0 Hz, 1H), 4.13 (d, J=12.9 Hz, 1H), 3.93 (d, J=9.9 Hz, 1H), 3.78-3.64 (m, 1H), 3.29-3.04 (m, 3H), 3.01-2.88 (m, 4H), 2.70 (s, 3H), 2.48-2.30 (m, 2H), 2.13-2.01 (m, 2H), 1.85 (ddd, J=16.8, 13.2, 3.9 Hz, 1H), 1.60 (s, 3H), 1.42 (d, J=7.0 Hz, 3H); m/z (ES+): 554 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 149 (82 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3-ethyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (93 mg, 0.44 mmol) in EtOAc (2 mL) and TEA (97 mg, 0.96 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (18 mg, yield: 14.9%); 1H NMR (400 MHz, CDCl3) δ 7.34 (s, 1H), 7.20 (s, 1H), 7.13 (d, J=6.8 Hz, 1H), 7.05 (dd, J=10.8, 7.1 Hz, 3H), 5.48 (q, J=7.0 Hz, 1H), 4.16-4.07 (m, 1H), 3.94 (d, J=9.9 Hz, 1H), 3.69 (dd, J=17.1, 7.3 Hz, 1H), 3.31-3.16 (m, 2H), 3.08 (d, J=12.5 Hz, 1H), 2.97-2.89 (m, 4H), 2.65 (s, 3H), 2.61 (d, J=7.6 Hz, 1H), 2.44-2.31 (m, 2H), 2.15-2.03 (m, 2H), 1.69 (s, 2H), 1.20 (t, J=7.6 Hz, 3H); m/z (ES+): 514 [M+H]+.
To a solution of triphosgene (47.2 mg, 0.16 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 33 (82 mg, 0.32 mmol), DMAP (3.9 mg, 0.032 mmol) and TEA (97 mg, 0.96 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3-isopropyl-5-(trifluoromethyl)phenyl)-N-methylethanamine (101 mg, 0.44 mmol) in EtOAc (2 mL) and TEA (97 mg, 0.96 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (15 mg, yield: 14%); 1H NMR (400 MHz, DMSO) δ 7.36 (s, 1H), 7.20 (s, 1H), 7.12 (t, J=7.7 Hz, 2H), 7.10-7.00 (m, 2H), 5.49 (q, J=7.1 Hz, 1H), 4.11 (d, J=12.9 Hz, 1H), 3.93 (d, J=9.9 Hz, 1H), 3.69 (dd, J=17.1, 7.3 Hz, 1H), 3.31-3.11 (m, 2H), 3.09 (t, J=12.1 Hz, 1H), 2.98-2.84 (m, 5H), 2.66 (s, 3H), 2.46-2.29 (m, 2H), 2.27-1.97 (m, 3H), 1.87-1.78 (m, 1H), 1.74-1.66 (m, 2H), 1.36 (d, J=7.0 Hz, 3H), 1.21 (d, J=6.8 Hz, 6H); m/z (ES+): 528 [M+H]+.
To a solution of triphosgene (25 mg, 0.095 mmol) in EtOAc (2 mL) at 0° C. was added solution of intermediate 154 (50 mg, 0.2 mmol) and TEA (75 mg, 0.72 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (60 mg, 0.22 mmol) in EtOAc (2 mL) and TEA (75 mg, 0.72 mmol). The reaction was stirred at 45° C. for 48 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the combined organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by silica gel chromatography to give the title compound (12 mg, yield: 11%); 1H NMR (400 MHz, CDCl3) δ ppm 7.76 (s, 1H), 7.54 (s, 2H), 7.18-7.16 (m, 1H), 7.14-7.12 (m, 1H), 6.98-6.80 (m, 1H), 5.46-5.38 (q, 1H), 3.38-36 (d, 1H), 4.18-4.12 (m, 1H), 4.10-3.98 (m, 1H), 3.47-3.43 (m, 1H), 3.12-3.04 (m, 1H), 2.65 (s, 3H), 2.55 (s, 3H), 2.47-2.43 (m, 3H), 1.93-1.91 (m, 1H), 1.25 (d, 3H); m/z (ES+): 546 [M+H]+.
To a solution of triphosgene (41.0 mg, 0.14 mmol) in EtOAc (2 mL) at 0° C. was added a solution of intermediate 157 (94.0 mg, 0.35 mmol), DMAP (4.0 mg, 0.03 mmol) and TEA (106.0 mg, 1.05 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methylethanamine (133.0 mg, 0.49 mmol) in EtOAc (2 mL) and TEA (106.0 mg, 1.05 mmol). The reaction was stirred at 40° C. for 16 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the organic layer was washed with 1 M aqueous HCl solution, dried over Na2SO4 and then concentrated in high vacuum. The residue was purified by Prep-HPLC to give the compound 83 (10.3 mg, yield: 5%) and the compound 84 (4.3 mg, yield: 2%).
Compound 83: 1H NMR (400 MHz, CDCl3) δ ppm 7.78 (s, 1H), 7.55 (s, 2H), 7.31-7.26 (m, 1H), 7.19-7.16 (m, 1H), 6.98-6.93 (m, 1H), 5.50 (q, J=6.8 Hz, 1H), 4.37 (d, J=10.0 Hz, 1H), 4.14 (d, J=12.4 Hz, 1H), 3.76-3.74 (m, 1H), 3.32 (d, J=11.6 Hz, 1H), 3.09-3.01 (m, 2H), 2.72 (s, 3H), 2.47-2.36 (m, 2H), 1.90-1.86 (m, 2H), 1.47 (d, J=7.2 Hz, 3H); m/z (ES+): 566 [M+H]+.
Compound 84: 1H NMR (400 MHz, CDCl3) δ ppm 7.66 (s, 1H), 7.34 (s, 2H), 7.19-7.17 (m, 1H), 7.12-7.09 (m, 1H), 6.89-6.85 (m, 1H), 5.52 (q, J=7.2 Hz, 1H), 4.34 (d, J=10.0 Hz, 1H), 4.16-4.09 (m, 1H), 3.58-3.56 (m, 1H), 3.32 (d, J=12.0 Hz, 1H), 3.04-3.01 (m, 1H), 2.92-2.88 (m, 1H), 2.75 (s, 3H), 2.39-2.28 (m, 2H), 1.83-1.76 (m, 2H), 1.45 (d, J=7.6 Hz, 3H); m/z (ES+): 566 [M+H]+.
20 To a solution of triphosgene (25.0 mg, 0.08 mmol) in EtOAc (2 mL) at 0° C. was added a solution of intermediate 162 (50.0 mg, 0.21 mmol), DMAP (4.0 mg, 0.03 mmol) and TEA (64.0 mg, 0.63 mmol) in EtOAc (2 mL). The mixture was stirred for 1.5 h at RT, followed by addition of (R)-1-(3,5-bis(trifluoromethyl)phenyl)-N-methyl ethanamine (80.0 mg, 0.29 mmol) in EtOAc (2 mL) and TEA (64.0 mg, 0.63 mmol). The reaction was stirred at 40° C. for 16 h and quenched with NH4Cl (aq). The resulting mixture was extracted with ETOAC; the organic layer was washed with 1 M aqueous HCl solution, washed with brine, dried over Na2SO4 and concentrated in vacuo. The residue was purified by Prep-HPLC to give the tilte compound (7.6 mg, yield: 7%). 1H NMR (400 MHz, CDCl3) δ ppm 7.70 (s, 1H), 7.66 (s, 2H), 7.19-7.13 (m, 2H), 6.97-6.93 (m, 2H), 5.85-5.76 (m, 2H), 5.25 (s, 1H), 3.80-3.77 (m, 2H), 3.52-3.49 (m, 2H), 2.94-2.91 (m, 2H), 2.53-2.50 (m, 5H), 1.47 (d, J=7.2 Hz, 3H). m/z (ES+): 532 [M+H]+.
U251 MG cells were seeded into black walled clear-bottom 384-well plates (Greiner Bio-One GmbH, Frickenhausen, Germany) at a density of 15,000 cells/well in 50 μl culture medium and cultured overnight in a 37° C. 5% CO2 incubator. The cells were then loaded with the calcium sensitive dye Fluo-4 (Invitrogen) at 1 μM in buffer, containing 0.04% Pluronic F-127 (Sigma-Aldrich), and 2.5 mM probenecid (Sigma-Aldrich) for 60 min in a humidified atmosphere of 5% CO2. Thereafter, cells were washed three times in washing buffer containing 20 mM HEPES and 2.5 mM probenecid pH 7.3. Serial dilutions of test compounds in assay buffer containing 2% dimethyl sulfoxide (final concentration in the cell plate is 0.5% DMSO) and/or agonist were then automatically pipetted into each test well, and the peak fluorescence intensity was recorded (lex, 488 nm; lem, 540 nm) by the FLIPR instrument (Molecular Devices) for approximately 5 min. To measure antagonist potency, cell plates were first incubated with the test compound and intracellular fluorescence recorded for 5 minutes to check a potential agonist effect of the test compound. Cell plates were quickly removed from the FLIPR instrument and incubated for additional 10 minutes at 37° C. before being moved back to the FLIPR instrument for Substance P (EC80) addition. The response was measured as the peak relative fluorescence change after agonist addition.
Compound relative %-effect was normalized to the maximal response evoked by 100 nM Aprepitant in presence of the EC80 of Substance P and the antagonist potency determined by non-linear regression using GraphPad Prism (version 5) or the four-parameter logistic model in XLfit (IDBS, Guilford, UK) for Microsoft Excel (Microsoft, Redmond, Wash.). The IC50 value is defined as the molar concentration of a test compound that produces a response midway between the fitted top and the fitted bottom. pIC50=−log IC50
Formula is listed as follows:
Fit (% Effect calculated)=(A+((B−A)/(1+((C/X)̂D))))
Ref: Sullivan E, Tucker E M, and Dale I L. Measurement of [Ca2+] using the Fluorometric Imaging Plate Reader (FLIPR). Methods Mol Biol 114:125-133, 1999.
Compounds of the present invention were tested according to example 100. Results are reported in Table 1.
The general procedure consists of an automated incubation system and LC-MS/MS analysis with human liver microsomes. Human liver microsomes are thawed rapidly in a waterbath at 37° C. and kept on ice until use. The human liver microsomes are diluted with 50 mM potassium phosphate buffer pH 7.4 to a protein concentration of 0.55 mg/mL. Test compounds and positive controls (Verapamil and Dextromethorphan, positive controls for CYP3A4 for CYP2D6 isoforms, respectively) are dissolved in methanol (or other appropriate solvent, if necessary) in order to obtain a 5 mM solution which is further diluted to the concentration of 50 μM. The stock solutions are prepared immediately before the test.
5 μL of 50 μM test compounds and controls are added to 445 μL-of the 0.55 mg/mL microsomes solution and the incubation mixture are pre-warmed at 37° C. for 5 minutes. The incubation reactions are initiated by adding 50 μL of pre-warmed NADPH regenerating system to the incubation mixtures. 50 μL-aliquots will be taken from the incubation mixtures at: 0, 3, 6, 9, 15 and 30 minutes and the reactions are stopped by adding 100 μL of ACN containing Rolipram, used as generic Internal Standard (IS). Samples are then diluted with 120 μL of Milli-Q water and centrifuged at 3000 rpm for 10 minutes, prior the LC-MS/MS analysis.
The integrated peak areas for test compounds at the selected time points are divided by the respective peak areas of the IS and the percent of parent remaining is calculated by normalizing the peak area ratio of parent to IS at 0 min. Observed rate constant (kobs) for parent degradation is calculated by determining the slope of the line of the graph of the natural log of percentage parent remaining versus time of incubation. From the rate of depletion (min−1) and volume of the incubation (mL), clearance is estimated. This is scaled for the protein in the incubation relative to that in the intact liver, for all species (mL/min/g liver).
Values for the Human in vitro Clint are expressed as mL/min/g liver.
Assuming a liver weight of 25.7 g/kg and a liver blood flow of 20.7 mL/min/kg, the hepatic extraction ratio, Eh, can be derived from the Human in vitro Clint:
E.g. a Human in vitro Clint of 2.4 mL/min/g liver corresponds to a hepatic extraction ratio of 75%.
Some compounds of the present invention were tested according to example 101. Results are reported in Table 1
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/CN2013/081889 | Aug 2013 | CN | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2014/067494 | 8/15/2014 | WO | 00 |
