Patent Application
20160199520
The present invention relates to a novel nitrogen-containing compound or a salt thereof, or a complex of the compound or the salt with a metal.
Integrins constitute a family of heterodimeric glycoprotein complexes composed of α and β subunits and are one kind of cell adhesion receptor involved mainly in cell adhesion to extracellular matrix and signal transduction from extracellular matrix. Among the integrins, integrins αvβ3 and αvβ5, which are vitronectin receptors, are known to be low expressed on epithelial cells or mature endothelial cells, but overexpressed on various tumor cells or neovessels. The overexpression of the integrins αvβ3 and αvβ5 is reportedly involved in the exacerbation of cancers, including infiltration, metastasis, accompanied by tumor angiogenesis, and highly related to the degree of malignancy (Non Patent Literature 1). For example, head and neck cancer, colorectal cancer, breast cancer, small-cell lung cancer, non-small cell lung cancer, glioblastoma, malignant melanoma, pancreatic cancer, and prostate cancer have been found as the cancers in which these integrins are overexpressed (Non Patent Literature 2). As for further integrin-related diseases, integrin overexpression on vascular endothelial cells during angiogenesis after ischemia has been revealed in ischemic diseases such as ischemic heart disease or peripheral vascular disease (Non Patent Literature 3).
The relationship between these diseases and integrin expression is interesting as a target for drugs. Treatment or imaging of disease site using low-molecular compounds (Patent Literatures 1 to 4) or compounds labeled with a radioisotope (Patent Literatures 5 to 7) has been reported.
For example, Non Patent Literatures 4 and 5 have been reported as attempts of imaging using a peptide ligand having an Arg-Gly-Asp (RGD) sequence, and Patent Literature 5 has been reported as an attempt using a non-peptide low molecule. Also, the visualization of human tumors using compounds carrying a positron nuclide 18F (Non Patent Literatures 6 and 7) or the like has been attempted (Non Patent Literatures 8 and 9).
Non Patent Literature 1: Nature Reviews Cancer, Vol. 10, p. 9-23, 2010
The conventional peptide having an RGD sequence is not sufficiently effective when applied to imaging or treatment, because of its low tumor accumulation and persistence as imaging and therapeutic drugs.
In terms of imaging, slow blood clearance may require about several days to 1 week for taking images with decreased blood values as a background. This is a serious disadvantage in consideration of the short half-life of a radioactive metal suitable for imaging. In terms of treatment using a therapeutic nuclide, long-term blood circulation means the dominant irradiation of the bone marrow and tends to cause severe bone marrow toxicity.
Thus, an object of the present invention is to provide an integrin-binding compound which has high accumulation and persistence in neovessels and tumors involving an integrin and exhibits fast blood clearance, and an agent for diagnosis or treatment, etc., comprising the compound as an active ingredient.
Under such circumstances, the present inventors have conducted diligent studies and consequently found that a complex of a compound represented by the following formula (1) or a salt thereof with a metal is useful as an agent for of diagnosis or treatment, etc., of a disease involving an integrin. The present inventors have also found that the compound represented by the following formula (1) or the salt thereof is useful as an intermediate for producing such a complex.
The present inventors have further found that a compound represented by the formula (S1a) shown below or a salt thereof is useful as an intermediate for producing the compound represented by the formula (1) or the salt thereof, or the complex of the compound or the salt with a metal. On the basis of these findings, the present invention has been completed.
Specifically, the present invention provides the following [1] to [26]: [1]A compound represented by the formula (1) or a salt thereof, or a complex of the compound or the salt with a metal:
wherein A1 represents a chelating agent; R1 represents a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; R2 represents a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; Z1, Z2, Z3, Z4, and Z5 are the same or different and each represent a nitrogen atom or CR3 wherein R3 represents a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkoxy group, or a group represented by the formula (2):
wherein R4 represents a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; n number of R5 and n number of R6 are the same or different and each represent a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, or an optionally protected carboxyl group; R7 represents a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; R8 represents a hydrogen atom, an optionally substituted C1-6 alkyl group, or a bond with L5; R9, R10, R11, and R12 are the same or different and each represent a hydrogen atom, a halogen atom, an optionally protected amino group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkylamino group, an optionally substituted di(C1-6 alkyl)amino group, or a bond with L5; or R8 and R9 together represent an optionally substituted C1-6 alkylene group, provided that any one of R8, R9, R10, R11, and R12 represents a bond with L3, and the other 4 moieties do not represent a bond with L5; L4 represents an optionally substituted divalent aromatic hydrocarbon group, an optionally substituted divalent heterocyclic group, or a bond; L5 represents an optionally substituted C1-6 alkylene group, an optionally substituted —O—C1-6 alkylene group wherein the left bond binds to L4, or an optionally substituted —NH—C1-6 alkylene group wherein the left bond binds to L4; m represents 0 or 1; n represents an integer of 1 to 3; and p represents 0 or 1, provided that when R8 bonds to L5, L4 represents an optionally substituted divalent aromatic hydrocarbon group, provided that at least one of Z1, Z2, Z3, Z4, and Z5 represents CR3a wherein R3a represents a group represented by the formula (2):
wherein R4, R5, R6, R7, R8, R9, R10, R11, R12, L4, L5, m, n, and p are as defined above;
L2 represents an optionally substituted C1-6 alkylene group; L3 represents an optionally substituted C1-6 alkylene group; and L1 represents a group represented by the formula (3):
wherein r number of R13 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; q×r number of R14 and q×r number of R15 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6 alkyl group; r number of R16 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or a group represented by the formula (4):
wherein s number of R17 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6 alkyl group; t number of R18 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group; t number of R19 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6 alkyl group; s represents an integer of 1 to 3; t represents an integer of 0 to 3; and R1, R2, Z1, Z2, Z3, Z4, Z5, L2, and L3 are as defined above; q represents an integer of 0 to 3; and r represents an integer of 0 to 3. [2] The compound or the salt thereof, or the complex of the compound or the salt with a metal according to [1], wherein Z1, Z2, Z4, and Z5 are the same or different and each represent CR3b wherein R3b represents a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, or an optionally substituted C1-6 alkoxy group; and Z3 represents CR3a wherein R3c represents a group represented by the formula (2a):
wherein n number of R5a and n number of R6a are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an optionally protected carboxyl group; R8a represents a hydrogen atom or an optionally substituted C1-6alkyl group; R9a represents a hydrogen atom; or R8a and R9a together represent an optionally substituted C1-6 alkylene group; L5a represents an optionally substituted C1-6 alkylene group; and L4 and n are as defined above.
[3] The compound or the salt thereof, or the complex of the compound or the salt with a metal according to [2], wherein R3c is a group represented by the formula (2b):
wherein R5a, R6a, L4, L5a, and n are as defined above. [4] The compound or a salt thereof, or a complex of the compound or the salt with a metal according to any one of [1] to [3], wherein A1 is a group having a polyazamacrocyclic structure, a group having a polyaminopolycarboxylic acid structure, or a group having a polyaminopolyphosphonic acid structure.
[5] The compound or the salt thereof, or the complex of the compound or the salt with a metal according to any one of [1] to [4], wherein A1 is a group represented by the formula (5), (6), (7), (8), (9), (10), (11), or (12):
wherein Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, and Ri are the same or different and each represent a hydrogen atom or a carboxyl-protecting group; X1, X2, X3, X4, X5, X6, X7, X8, X9, Y1, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 are the same or different and each represent an optionally substituted C1-6 alkylene group or an optionally substituted C3-8 cycloalkylene group; X10 represents an optionally substituted C1-6 alkylene group; X4a and X8a are the same or different and each represent an optionally substituted C1-6 alkanetriyl group: and Q1 represents an oxygen atom or a sulfur atom.
[6] The compound or the salt thereof, or the complex of the compound or the salt with a metal according to any one of [1] to [5], wherein A is a group represented by the formula (5a), (6a), (7a), (8a), (8b), (8c), (9a), (10a), (10b), (11a), (11b), (11c), or (12a):
[7] The compound or the salt thereof, or the complex of the compound or the salt with a metal according to [1], wherein the compound represented by the formula (1) or the salt thereof is a compound or a salt thereof selected from the group consisting of 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid,
wherein A1, R1, R2, Z1, Z2, Z3, Z4, Z5, L1, L2, and L3 are as defined above,
allowing a compound represented by the formula (S1a) or a salt thereof:
wherein R1, R2, Z1, Z2, Z3, Z4, Z5, L1, L2, and L3 are as defined above, to react with a compound represented by the formula (S2):
A1-RB (S2)
wherein RB represents a hydroxyl group or a leaving group; and A1 is as defined above, or a compound represented by the formula (83):
B1-N═C=Q1 (S3)
wherein B1 represents a chelate residue: and Q1 is as defined above.
[18]A compound represented by the formula (S1a) or a salt thereof:
wherein R1, R2, Z1, Z2, Z3, Z4, Z5, L1, L2, and L3 are as defined above.
[19] The compound or the salt thereof according to [18], wherein Z1, Z2, Z4, and Z5 are the same or different and each represent CR3b wherein R3b is as defined above; and Z3 represents CR3c wherein R3e is as defined above.
[20] The compound or the salt thereof according to [18] or [19], wherein R3e represents a group represented by the formula (2b):
wherein R5a, R6aa, L4, L5a, and n are as defined above.
[21] The complex according to [8] or [9] for use in the treatment of a disease involving an integrin.
[22] The complex according to [12] or [13] for use in the diagnosis of a disease involving an integrin.
[23] Use of the complex according to [8] or [9] for producing an agent for the treatment of a disease involving an integrin.
[24] Use of the complex according to [12] or [13] for producing an agent for the diagnosis of a disease involving an integrin.
[25]A method for treating a disease involving an integrin, comprising administering the complex according to [8] or [9].
[26]A method for diagnosing a disease involving an integrin, comprising administering the complex according to [12] or [13].
The complex of the compound represented by the formula (1) or a salt thereof with a metal of the present invention has high accumulation and persistence in integrin-expressing cells such as cancer cells and exhibits fast blood clearance. Therefore, the complex is useful for a procedure of diagnosis or treatment, etc., of a disease involving an integrin. Moreover, the compound represented by the formula (1) or the salt thereof of the present invention is useful as an intermediate for producing the complex. Furthermore, the compound represented by the formula (S1a) of the present invention or a salt thereof is useful as an intermediate for producing the compound represented by the formula (1) or the salt thereof, or the complex of the compound or the salt with a metal of the present invention.
Hereinafter, the present invention will be described in detail. The group represented by the following formula:
used in the present invention means a group in which x number of A are bonded.
The number of A: x
x number of A may be the same or may be different.
In the present invention, each term has the following meaning, unless otherwise specified.
The halogen atom means a fluorine atom, a chlorine atom, a bromine atom, or an iodine atom.
The C1-4alkyl group means a methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, isobutyl, or tert-butyl group. The C1-6alkyl group means a linear or branched C1-6 alkyl group such as methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, isobutyl, tert-butyl, pentyl, isopentyl, 2-methylbutyl, 2-pentyl, 3-pentyl, and hexyl groups. The aryl group means a C6-10 aryl group such as phenyl and naphthyl groups. The ar-C1-6 alkyl group means a C6-10 ar-C1-6 alkyl group such as benzyl, diphenylmethyl, trityl, phenethyl, 2-phenylpropyl, 3-phenylpropyl, and naphthylmethyl groups.
The C1-4 alkylene group means a linear or branched C1-4 alkylene group such as methylene, ethylene, propylene, and butylene groups. The C1-6 alkylene group means a linear or branched C1-6 alkylene group such as methylene, ethylene, propylene, butylene, pentylene, and hexylene groups. The —O—C1-6 alkylene group means a group in which the C1-6 alkylene group is bonded to an oxygen atom, such as oxyethylene, oxypropylene, and oxybutylene groups. The —NH—C1-6 alkylene group means a group in which the C1-6 alkylene group is bonded to an amino group, such as aminoethylene, aminopropylene, and aminobutylene groups. The C3-8 cycloalkylene group means a cyclopropylene, cyclobutylene, cyclopentylene, cyclohexylene, cycloheptylene, or cyclooctylene group.
The C1-4alkanetriyl group means a linear or branched C1-4alkanetriyl group such as methanetriyl, ethanetriyl, propanetriyl, and butanetriyl groups. The C1-6 alkanetriyl group means a linear or branched C1-6 alkanetriyl group such as methanetriyl, ethanetriyl, propanetriyl, butanetriyl, pentanetriyl, and hexanetriyl groups.
The C1-6 alkoxy group means a linear, cyclic, or branched C1-6 alkyloxy group such as methoxy, ethoxy, propoxy, isopropoxy, cyclopropoxy, butoxy, isobutoxy, sec-butoxy, tert-butoxy, cyclobutoxy, pentyloxy, and hexyloxy groups. The C1-6alkoxy-C1-6alkyl group means a C1-6 alkyloxy-C1-6alkyl group such as methoxymethyl and 1-ethoxyethyl groups.
The C1-6alkylamino group means a linear, branched, or cyclic C1-6 alkylamino group such as methylamino, ethylamino, propylamino, isopropylamino, cyclopropylamino, butylamino, sec-butylamino, tert-butylamino, cyclobutylamino, pentylamino, cyclopentylamino, hexylamino, and cyclohexylamino groups. The di(C1-6 alkyl)amino group means a linear, branched, or cyclic di(C1-6 alkyl)amino group such as dimethylamino, diethylamino, dipropylamino, diisopropylamino, dibutylamino, di(tert-butyl)amino, dipentylamino, dihexylamino, (ethyl)(methyl)amino, (methyl)(propyl)amino, (cyclopropyl)(methyl)amino, (cyclobutyl)(methyl)amino, and (cyclohexyl)(methyl)amino groups.
The C2-6 alkanoyl group means a linear or branched C2-6 alkanoyl group such as acetyl, propionyl, valeryl, isovaleryl, and pivaloyl groups. The aroyl group means a C6-10 aroyl group such as benzoyl and naphthoyl groups. The heterocyclic carbonyl group means a monocyclic or bicyclic heterocyclic carbonyl group such as furoyl, thenoyl, pyrrolidinylcarbonyl, piperidinylcarbonyl, piperazinylcarbonyl, morpholinylcarbonyl, and pyridinylcarbonyl groups. The acyl group means a formyl group, a succinyl group, a glutaryl group, a maleoyl group, a phthaloyl group, a C2-6 alkanoyl group, an aroyl group, or a heterocyclic carbonyl group.
The C1-6 alkoxycarbonyl group means a linear or branched C1-6 alkyloxycarbonyl group such as methoxycarbonyl, ethoxycarbonyl, isopropoxycarbonyl, tert-butoxycarbonyl, and 1,1-dimethylpropoxycarbonyl groups. The ar-C1-6 alkoxycarbonyl group means a C6-10 ar-C1-6alkyloxycarbonyl group such as benzyloxycarbonyl and phenethyloxycarbonyl groups.
The C1-6alkylthio group means a C1-6 alkylthio group such as methylthio, ethylthio, propylthio, and butylthio groups. The C1-6 alkylsulfonyl group means a C1-6 alkylsulfonyl group such as methylsulfonyl, ethylsulfonyl, and propylsulfonyl groups. The arylsulfonyl group means a C6-10 arylsulfonyl group such as benzenesulfonyl, p-toluenesulfonyl, and naphthalenesulfonyl groups. The C1-6 alkylsulfonyloxy group means a C1-6 alkylsulfonyloxy group such as methylsulfonyloxy and ethylsulfonyloxy groups. The arylsulfonyloxy group means a C6-10 arylsulfonyloxy group such as benzenesulfonyloxy and p-toluenesulfonyloxy groups.
The monocyclic nitrogen-containing heterocyclic group means a monocyclic heterocyclic group containing only a nitrogen atom as a heteroatom constituting the ring, such as aziridinyl, azetidinyl, pyrrolidinyl, pyrrolinyl, pyrrolyl, piperidyl, tetrahydropyridyl, dihydropyridyl, pyridyl, homopiperidinyl, octahydroazocinyl, imidazolidinyl, imidazolinyl, imidazolyl, pyrazolidinyl, pyrazolinyl, pyrazolyl, piperazinyl, pyrazinyl, pyridazinyl, pyrimidinyl, homopiperazinyl, triazolyl, and tetrazolyl groups. The monocyclic oxygen-containing heterocyclic group means a monocyclic heterocyclic group containing only an oxygen atom as a heteroatom constituting the ring, such as oxetanyl, tetrahydrofuranyl, furanyl, tetrahydropyranyl, pyranyl, 1,3-dioxanyl, and 1,4-dioxanyl groups. The monocyclic sulfur-containing heterocyclic group means a monocyclic heterocyclic group containing only a sulfur atom as a heteroatom constituting the ring, such as a thienyl group. The monocyclic nitrogen- and oxygen-containing heterocyclic group means a monocyclic heterocyclic group containing only a nitrogen atom and an oxygen atom as heteroatoms constituting the ring, such as oxazolyl, isoxazolyl, oxadiazolyl, morpholinyl, and oxazepanyl groups. The monocyclic nitrogen- and sulfur-containing heterocyclic group means a monocyclic heterocyclic group containing only a nitrogen atom and a sulfur atom as heteroatoms constituting the ring, such as thiazolyl, isothiazolyl, thiadiazolyl, thiomorpholinyl, 1-oxidothiomorpholinyl, and 1,1-dioxidothiomorpholinyl groups. The monocyclic heterocyclic group means a monocyclic nitrogen-containing heterocyclic group, a monocyclic oxygen-containing heterocyclic group, a monocyclic sulfur-containing heterocyclic group, a monocyclic nitrogen- and oxygen-containing heterocyclic group, or a monocyclic nitrogen- and sulfur-containing heterocyclic group.
The bicyclic nitrogen-containing heterocyclic group means a bicyclic heterocyclic group containing only a nitrogen atom as a heteroatom constituting the rings, such as indolinyl, indolyl, isoindolinyl, isoindolyl, benzimidazolyl, indazolyl, benzotriazolyl, pyrazolopyridinyl, quinolyl, tetrahydroquinolinyl, quinolyl, tetrahydroisoquinolinyl, isoquinolinyl, quinolizinyl, cinnolinyl, phthalazinyl, quinazolinyl, dihydroquinoxalinyl, quinoxalinyl, naphthyridinyl, purinyl, pteridinyl, and quinuclidinyl groups. The bicyclic oxygen-containing heterocyclic group means a bicyclic heterocyclic group containing only an oxygen atom as a heteroatom constituting the rings, such as 2,3-dihydrobenzofuranyl, benzofuranyl, isobenzofuranyl, chromanyl, chromenyl, isochromanyl, 1,3-benzodioxolyl, 1,3-benzodioxanyl, and 1,4-benzodioxanyl groups. The bicyclic sulfur-containing heterocyclic group means a bicyclic heterocyclic group containing only a sulfur atom as a heteroatom constituting the rings, such as 2,3-dihydrobenzothienyl and benzothienyl groups. The bicyclic nitrogen- and oxygen-containing heterocyclic group means a bicyclic heterocyclic group containing only a nitrogen atom and an oxygen atom as heteroatoms constituting the rings, such as benzoxazolyl, benzisoxazolyl, benzoxadiazolyl, benzomorpholinyl, dihydropyranopyridyl, dioxolopyridyl, furopyridinyl, dihydrodioxinopyridyl, and dihydropyridooxazinyl groups. The bicyclic nitrogen- and sulfur-containing heterocyclic group means a bicyclic heterocyclic group containing a nitrogen atom and a sulfur atom as heteroatoms constituting the rings, such as benzothiazolyl, benzisothiazolyl, and benzothiadiazolyl groups. The bicyclic heterocyclic group means a bicyclic nitrogen-containing heterocyclic group, a bicyclic oxygen-containing heterocyclic group, a bicyclic sulfur-containing heterocyclic group, a bicyclic nitrogen- and oxygen-containing heterocyclic group, or a bicyclic nitrogen- and sulfur-containing heterocyclic group.
The heterocyclic group means a monocyclic heterocyclic group or a bicyclic heterocyclic group.
The divalent aromatic hydrocarbon group means an optionally partially hydrogenated divalent aromatic hydrocarbon group such as phenylene, pentalenylene, indanylene, indenylene, and naphthylene groups. The divalent heterocyclic group means a group formed by the further removal of one hydrogen atom from a heterocyclic group, such as pyrrolediyl, imidazolediyl, triazolediyl, tetrazolediyl, pyrrolidinediyl, imidazolidinediyl, furandiyl, thiophenediyl, oxazolediyl, thiazolediyl, pyridinediyl, pyrimidinediyl, indolediyl, quinolinediyl, and isoquinolinediyl groups.
The amino-protecting group includes all groups which may be used as usual protecting groups for the amino group. Examples thereof include groups described in W. Greene et al., Protective Groups in Organic Synthesis, Vol. 4, p. 696-926, 2007, John Wiley & Sons, INC. Specific examples thereof include ar-C1-6 alkyl groups, C1-6alkoxy-C1-6 alkyl groups, acyl groups, C1-6 alkoxycarbonyl groups, ar-C1-6 alkoxycarbonyl groups, C1-6 alkylsulfonyl groups, arylsulfonyl groups, and silyl groups.
The hydroxyl-protecting group includes all groups which may be used as usual protecting groups for the hydroxyl group. Examples thereof include groups described in W. Greene et al., Protective Groups in Organic Synthesis, Vol. 4, p. 16-366, 2007, John Wiley & Sons, INC. Specific examples thereof include C1-6 alkyl groups, ar-C1-6 alkyl groups, C1-6alkoxy-C1-6 alkyl groups, acyl groups, C1-6 alkoxycarbonyl groups, ar-C1-6 alkoxycarbonyl groups, C1-6 alkylsulfonyl groups, arylsulfonyl groups, silyl groups, a tetrahydrofuranyl group, and a tetrahydropyranyl group.
The carboxyl-protecting group includes all groups which may be used as usual protecting groups for the carboxyl group. Examples thereof include groups described in W. Greene et al., Protective Groups in Organic Synthesis, Vol. 4, p. 533-646, 2007, John Wiley & Sons, INC. Specific examples thereof include C1, alkyl groups, aryl groups, ar-C1-6 alkyl groups, C1-6 alkoxy-C1-6 alkyl groups, and silyl groups.
The thiol-protecting group includes all groups which may be used as usual protecting groups for the thiol group. Examples thereof include groups described in W. Greene et al., Protective Groups in Organic Synthesis, Vol. 4, p. 647-695, 2007, John Wiley & Sons, INC. Specific examples thereof include C1-6 alkyl groups, ar-C1-6 alkyl groups, C1-6 alkoxy-C1-6 alkyl groups, acyl groups, and silyl groups.
The silyl group means, for example, a trimethylsilyl, triethylsilyl, tributylsilyl, or tert-butyldimethylsilyl group.
Examples of the leaving group include halogen atoms, C1-6alkylsulfonyloxy groups, and arylsulfonyloxy groups. The C1-6 alkylsulfonyloxy groups and the arylsulfonyloxy groups are each optionally substituted.
The chelate group means an organic group capable of forming a chelate bond with a metal. Specific examples thereof include groups having an alkylenediamine structure, a bipyridine structure, an alkylenediamine tetraacetic acid structure, a phenanthroline structure, a porphyrin structure, a crown ether structure, a polyazamacrocyclic structure, a polyaminopolycarboxylic acid structure, or a polyaminopolyphosphonic acid structure. The polyazamacrocyclic structure means a structure having a cyclic backbone in which 3 to 5 nitrogen atoms are interconnected by the same number of C1-6 alkylene groups as the number of the nitrogen atoms. Examples thereof include cyclen, cyclam, bridged-cyclam, ET-cyclam, and diamsar. The polyaminopolycarboxylic acid structure means a structure having a backbone in which 3 to 5 nitrogen atoms are interconnected by the same number of C1-6 alkylene groups as the number of the nitrogen atoms resulting in closure and a C1-6 alkyl group substituted by at least one carboxyl group is bonded to each of at least two of the nitrogen atoms, or a structure having a backbone in which 2 to 4 nitrogen atoms are interconnected by C1-6 alkylene groups and/or C3-8 cycloalkylene groups fewer by one than the number of the nitrogen atoms resulting in open chain and a C1-6 alkyl group substituted by at least one carboxyl group is bonded to each of at least two of the nitrogen atoms. Examples thereof include DOTA, DO3A, DO2A, CB-DO2A, TETA, TE3A, TE2A, CB-TE2A, NOTA, NODASA, NODAGA, BCNOTA, EDTA, DTPA, 1B4M-DTPA, and CHX-DTPA. The polyaminopolyphosphonic acid structure means a backbone in which at least one carboxyl group in the backbone of the polyaminopolycarboxylic acid structure is replaced with a phosphono group. Examples thereof include DOTP, NOTP, EDTP, HDTP, and NTP. The group having a polyazamacrocyclic structure, a polyaminopolycarboxylic acid structure, or a polyaminopolyphosphonic acid structure forms a coordinate bond with a metal as the chelate group through a plurality of nitrogen atoms, carboxyl groups, and/or phosphono groups to form a complex, while the N terminus of L1 is linked to the metal via a carboxyl group which is not involved in the coordination, a phosphono group which is not involved in the coordination, or a side chain introduced onto the backbone. Such a side chain is preferably a side chain capable of binding to L1 easily, and groups having an active group such as an anhydride group, a bromoacetamide group, an iodoacetamide group, an isothiocyanato group, a N-hydroxysuccinimide group, or a maleimide group are known (Liu et al., Advanced Drug Delivery Reviews 60: 1347-1370 (2008)).
The halogenated hydrocarbons mean methylene chloride, chloroform, and dichloroethane. The ethers mean diethyl ether, diisopropyl ether, dioxane, tetrahydrofuran, anisole, ethylene glycol dimethyl ether, diethylene glycol dimethyl ether, and diethylene glycol diethyl ether. The alcohols mean methanol, ethanol, propanol, 2-propanol, butanol, and 2-methyl-2-propanol. The ketones mean acetone, 2-butanone, 4-methyl-2-pentanone, and methyl isobutyl ketone. The esters mean methyl acetate, ethyl acetate, propyl acetate, and butyl acetate. The amides mean N,N-dimethylformamide, N,N-dimethylacetamide, and N-methylpyrrolidone. The nitriles mean acetonitrile and propionitrile. The sulfoxides mean dimethyl sulfoxide and sulfolane. The aromatic hydrocarbons mean benzene, toluene, and xylene.
The inorganic base means sodium hydroxide, potassium hydroxide, sodium tert-butoxide, potassium tert-butoxide, sodium bicarbonate, sodium carbonate, potassium carbonate, or cesium carbonate. The organic base means triethylamine, N,N-diisopropylethylamine, 1,8-diazabicyclo(5.4.0)undec-7-ene (DBU), 4-dimethylaminopyridine, or N-methylmorpholine.
Examples of the salts of the compound represented by the formula (1) and the compound represented by the formula (S1a) can include usually known salts of basic groups such as an amino group or acidic groups such as a hydroxyl group or a carboxyl group. Examples of the salts of basic groups include: salts with mineral acids such as hydrochloric acid, hydrobromic acid, nitric acid, and sulfuric acid; salts with organic carboxylic acids such as formic acid, acetic acid, citric acid, oxalic acid, fumaric acid, maleic acid, succinic acid, malic acid, tartaric acid, aspartic acid, trichloroacetic acid, and trifluoroacetic acid; and salts with sulfonic acids such as methanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, mesitylenesulfonic acid, and naphthalenesulfonic acid. Examples of the salts of acidic groups include: salts with alkali metals such as sodium and potassium; salts with alkaline earth metals such as calcium and magnesium; ammonium salts; and salts with nitrogen-containing organic bases such as trimethylamine, triethylamine, tributylamine, pyridine, N,N-dimethylaniline, N-methylpiperidine, N-methylmorpholine, diethylamine, dicyclohexylamine, procaine, dibenzylamine, N-benzyl-β-phenethylamine, 1-ephenamine, and N,N′-dibenzylethylenediamine. Among these salts, preferred examples of the salt include pharmaceutically acceptable salts.
The procedure means, for example, the diagnosis, prevention, or treatment of various diseases. The diagnosis means, for example, the judgment of a disease as being a target or the judgment of a condition of the target disease. The prevention means, for example, the inhibition of development, reduction in the risk of development, or the delay of development. The treatment means, for example, the amelioration of the target disease or condition or the suppression of progression thereof. The agent means a substance which is applied for the purpose of the procedure.
The compound of the present invention is represented by the formula (1):
wherein R1, R2, Z1, Z2, Z3, Z4, Z5, L1, L2, L3, and A1 are as defined above.
A1 is a chelate group. The chelate group represented by A1 is preferably a group having a polyazamacrocyclic structure, a group having a polyaminopolycarboxylic acid structure, or a group having a polyaminopolyphosphonic acid structure, more preferably a group having a polyaminopolycarboxylic acid structure, further preferably a group represented by the formula (5), (6), (7), (8), (9), (10), (11), or (12):
wherein Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, Ri, X1, X2, X3, X4, X4a, X5, X6, X7, X8, X9, X10, Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, and Q1 are as defined above,
particularly preferably a group represented by the formula (5a), (6a), (7a), (8a), (8b), (8c), (9a), (10a), (10b), (11a), (11b), (11c), or (12a):
most preferably a group represented by the formula (5a), (8c), or (12a):
Each of Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, and Ri is a hydrogen atom or a carboxyl-protecting group. Each of Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, and Ri is preferably a hydrogen atom or a C1-6 alkyl group, more preferably a hydrogen atom.
Each of X1, X2, X3, X4, X5, X6, X7, X8, X9, Y1, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 is an optionally substituted C1-6 alkylene group or an optionally substituted C3-8 cycloalkylene group. Each of X1, X2, X3, X4, X5, X6, X7, X8, X9, Y1, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 is preferably an optionally substituted C1-4 alkylene group, more preferably a C1-4 alkylene group, further preferably a methylene group, an ethylene group, or a propylene group. Examples of the substituent for the C1-6 alkylene group or the C3-8 cycloalkylene group represented by each of X1, X2, X3, X4, X5, X6, X7, X8, X9, Y1, Y2, Y3, Y4, Y5, Y6, Y7, and Y8 include one or more groups selected from substituent group α.
Substituent group α: a halogen atom, a cyano group, a carbamoyl group, a sulfo group, an optionally protected amino group, an optionally protected hydroxyl group, an optionally protected carboxyl group, a C1-6 alkyl group, a C1-6 alkoxy group, an aryl group, a heterocyclic group, and an oxo group.
X10 is an optionally substituted C1-6 alkylene group. X10 is preferably an optionally substituted C1-4 alkylene group, more preferably a C1-4alkylene group, further preferably an ethylene group. Examples of the substituent for the C1-6 alkylene group represented by X10 include one or more groups selected from substituent group α.
Each of X4a and X8a is an optionally substituted C1-6 alkanetriyl group. Each of X4a and X8a is preferably an optionally substituted C1-4 alkanetriyl group, more preferably a C1-4alkanetriyl group. Examples of the substituent for the C1-6alkanetriyl group represented by each of X4a and X8a include one or more groups selected from substituent group α.
R1 is a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group.
R1 is preferably a hydrogen atom or an optionally substituted C1-6 alkyl group, more preferably a hydrogen atom.
Examples of the substituent for the C1-6 alkyl group represented by R1 include one or more groups selected from substituent group α.
R is a hydrogen atom, an optionally substituted C1-6-alkyl group, or an amino-protecting group. R2 is preferably a hydrogen atom or an optionally substituted C1-6 alkyl group, more preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group represented by R2 include one or more groups selected from substituent group α.
Each of Z1, Z2, Z3, Z4, and Z5 is a nitrogen atom or CR3 wherein R3 is as defined above, provided that at least one of Z1, Z2, Z3, Z4, and Z5 is CR3a wherein R3a is as defined above.
Z1 and Z5 are the same or different and are each preferably CR3 wherein R3 is as defined above, more preferably CR3b wherein R3b is as defined above, further preferably CR3d wherein R3d represents a hydrogen atom or an optionally substituted C1-6 alkyl group.
Z2 is preferably a nitrogen atom or CR3 wherein R3 is as defined above, more preferably a nitrogen atom or CR3b wherein R3b is as defined above, further preferably CR3b wherein R3b is as defined above, particularly preferably CR3d wherein R3d is as defined above.
Z3 is preferably CR3 wherein R3 is as defined above. When Z2 is a nitrogen atom, Z3 is preferably CR3b wherein R3b is as defined above. When Z2 is CR3 wherein R3 is as defined above, Z3 is preferably CR3a wherein R3a is as defined above. Z3 is further preferably CR3c wherein R3c is as defined above, particularly preferably CR3e wherein R3e represents a group represented by the formula (2c):
wherein n1R5b and n1R6b are the same or different and each represent a hydrogen atom or an optionally protected carboxyl group; L4a represents an optionally substituted divalent aromatic hydrocarbon group or a bond; n1 represents 1 or 2; and L5a is as defined above.
Z4 is preferably CR3 wherein R3 is as defined above. When Z2 is a nitrogen atom, Z4 is more preferably CR3a wherein R3a is as defined above. When Z2 is CR3 wherein R3 is as defined above, Z4 is more preferably CR3b wherein R3b is as defined above. Z4 is further preferably CR3b wherein R3b is as defined above, particularly preferably CR3d wherein R3d is as defined above.
Preferred examples of the structure of the 6-membered ring having Z1, Z2, Z3, Z4, and Z5 include the following structures:
wherein R3 is as defined above.
More preferred examples of the structure of the 6-membered ring having Z1, Z2, Z3, Z4, and Z5 include the following structures:
wherein R3b and R3c are as defined above.
Further preferred examples of the structure of the 6-membered ring having Z1, Z2, Z3, Z4, and Z5 include the following structure:
wherein R3b and R3c are as defined above.
R3 is a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkoxy group, or a group represented by the formula (2):
wherein R4, R5, R6, R7, R8, R9, R10, R11, R12, L4, L5, m, n, and p are as defined above.
Examples of the substituent for the C1-6 alkyl group or the C1-6 alkoxy group represented by R3 include one or more groups selected from substituent group α.
R3b is a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, or an optionally substituted C1-6 alkoxy group. R3b is preferably a hydrogen atom or an optionally substituted C1-6 alkyl group. Examples of the substituent for the C1-6 alkyl group or the C1-6 alkoxy group represented by R3b include one or more groups selected from substituent group α.
R3c is a group represented by the formula (2a):
wherein R5a, R6a, R8a, R9a, L4, L5a, and n are as defined above.
R3c is preferably a group represented by the formula (2b):
wherein R5a, R6a, L4, L5a, and n are as defined above, more preferably a group represented by the formula (2c):
wherein R5b, R6b, L4, L5a, and n1 are as defined above.
R4 is a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group. R4 is preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group represented by R4 include one or more groups selected from substituent group α.
n number of R5 and n number of R6 are the same or different and each represent a hydrogen atom, a halogen atom, an optionally substituted C1-6 alkyl group, or an optionally protected carboxyl group. R5 and R6 are the same or different and are each preferably a hydrogen atom, an optionally substituted C1-6 alkyl group, or an optionally protected carboxyl group. Examples of the substituent for the C1-6 alkyl group represented by each of R5 and R6 include one or more groups selected from substituent group α.
n number of R5a and n number of R6a are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an optionally protected carboxyl group. R5a is preferably a hydrogen atom or an optionally protected carboxyl group. R6a is preferably a hydrogen atom or an optionally protected carboxyl group. Examples of the substituent for the C1-6 alkyl group represented by each of R5a and R6a include one or more groups selected from substituent group α.
n1 number of R5b and n1 number of R6b are the same or different and each represent a hydrogen atom or an optionally protected carboxyl group. R5b is preferably a hydrogen atom. R6b is preferably a hydrogen atom.
R7 is a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group. R7 is preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group represented by R7 include one or more groups selected from substituent group α.
R8 is a hydrogen atom, an optionally substituted C1-6 alkyl group, a bond with L5, or an optionally substituted C1-6 alkylene group together with R9. R8 is preferably a hydrogen atom, an optionally substituted C1-6 alkyl group, or an optionally substituted C1-6 alkylene group together with R9, more preferably an optionally substituted C1-6 alkylene group together with R9. Examples of the substituent for the C1-6 alkyl group represented by R8 include one or more groups selected from substituent group α. Examples of the substituent for the C1-6 alkylene group formed together with R9 include one or more groups selected from substituent group β.
Substituent group β: a halogen atom, a cyano group, a carbamoyl group, a sulfo group, an optionally protected amino group, an optionally protected hydroxyl group, an optionally protected carboxyl group, a C1-6 alkyl group, a C1-6 alkoxy group, an aryl group, a C1-6 alkylamino group, a di(C1-6 alkyl)amino group, a heterocyclic group, and an oxo group.
R8a is a hydrogen atom or an optionally substituted C1-6 alkyl group; R9a is a hydrogen atom; or R8a and R9a together represent an optionally substituted C1-6 alkylene group. Preferably, R8a and R9a together represent an optionally substituted C1-6 alkylene group. Examples of the substituent for the C1-6 alkyl group represented by R8a include one or more groups selected from substituent group α.
Examples of the substituent for the C1-6 alkylene group formed together with R9a include one or more groups selected from substituent group β.
R9 is a hydrogen atom, a halogen atom, an optionally protected amino group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkylamino group, an optionally substituted di(C1-6alkyl)amino group, a bond with L5, or an optionally substituted C1-6alkylene group together with R8. R9 is preferably a hydrogen atom or an optionally substituted C1-6 alkylene group together with R8, more preferably an optionally substituted C1-6 alkylene group together with R8. Examples of the substituent for the C1-6 alkyl group represented by R9 include one or more groups selected from substituent group α. Examples of the substituent for the C1-6 alkylene group formed together with R8 include one or more groups selected from substituent group β.
R10 and R11 are the same or different and each represent a hydrogen atom, a halogen atom, an optionally protected amino group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkylamino group, an optionally substituted di(C1-6 alkyl)amino group, or a bond with L5. R10 and R11 are the same or different and are each preferably a hydrogen atom, a halogen atom, or an optionally substituted C1-6 alkyl group, more preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group, the C1-6 alkylamino group, or the di(C1-6alkyl)amino group represented by each of R10 and R11 include one or more groups selected from substituent group α.
R12 is a hydrogen atom, a halogen atom, an optionally protected amino group, an optionally substituted C1-6 alkyl group, an optionally substituted C1-6 alkylamino group, an optionally substituted di(C1-6 alkyl)amino group, or a bond with L5. R12 is preferably a bond with L5.
Examples of the substituent for the C1-6 alkyl group, the C1-6 alkylamino group, or the di(C1-6alkyl)amino group represented by R12 include one or more groups selected from substituent group α.
Preferred examples of the structure of the 6-membered ring having R8, R9, R10, R11, and R12 include the following structure:
wherein R8a and R9a are as defined above.
More preferred examples of the structure of the 6-membered ring having R8, R9, R10, R11, and R12 include the following structures:
Further preferred examples of the structure of the 6-membered ring having R8, R9, R10, R11, and R12 include the following structure:
L4 is an optionally substituted divalent aromatic hydrocarbon group, an optionally substituted divalent heterocyclic group, or a bond. L4 is preferably a divalent aromatic hydrocarbon group, a divalent heterocyclic group, or a bond, more preferably an optionally substituted divalent aromatic hydrocarbon group or a bond, further preferably a phenylene group, an indolediyl group, or a bond. Examples of the substituent for the divalent aromatic hydrocarbon group or the divalent heterocyclic group represented by L4 include one or more groups selected from substituent group α.
L5 is an optionally substituted C1-6 alkylene group, an optionally substituted —O—C1-6 alkylene group, or an optionally substituted —NH—C1-6 alkylene group.
L5 is preferably an optionally substituted C1-6 alkylene group. Examples of the substituent for the C1-6 alkylene group, the —O—C1-6 alkylene group, or the —NH—C1-6 alkylene group represented by L5 include one or more groups selected from substituent group α.
L5a is an optionally substituted C1-6 alkylene group. Examples of the substituent for the C1-6 alkylene group represented by L5a include one or more groups selected from substituent group α.
m is 0 or 1. m is preferably 1.
n is an integer of 1 to 3. n is preferably 1 or 2.
p is 0 or 1. p is preferably 1.
L2 is an optionally substituted C1-6 alkylene group. L2 is preferably a C1-6 alkylene group, more preferably a C1-4 alkylene group. Examples of the substituent for the C1-6 alkylene group represented by L2 include one or more groups selected from substituent group α.
L3 is an optionally substituted C1-6 alkylene group. L is preferably a C1-6 alkylene group, more preferably a C1-4alkylene group. Examples of the substituent for the C1-6 alkylene group represented by L3 include one or more groups selected from substituent group α.
L1 is a group represented by the formula (3):
wherein R13, R14, R15, R16, q, and r are as defined above. L1 is preferably a group represented by the formula (3a):
wherein r1 number of R16a are the same or different and each represent an optionally sulfo group-substituted C1-4 alkyl group; and r1 represents 1 or 2.
r number of R13 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group. Each of r number of R13 is preferably a hydrogen atom or an amino-protecting group, more preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group represented by each of r number of R13 include one or more groups selected from substituent group α.
q×r number of R14 and q×r number of R15 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6 alkyl group. Each of q×r number of R14 and q×r number of R15 is preferably a hydrogen atom or a C1-6 alkyl group, more preferably a hydrogen atom. Examples of the substituent for the C1-6 alkyl group represented by each of q×r number of R14 and q×r number of R15 include one or more groups selected from substituent group α.
r number of R16 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or a group represented by the formula (4):
wherein R1, R2, R17, R18, R19, Z1, Z2, Z3, Z4, Z5, L2, L3, s, and t are as defined above.
R16 is preferably a hydrogen atom, an optionally substituted C1-4 alkyl group, or a group represented by the formula (4), more preferably a hydrogen atom, an optionally sulfo group-substituted C1-4 alkyl group, or a group represented by the formula (4), further preferably an optionally sulfo group-substituted C1-4 alkyl group. Examples of the substituent for the C1-6 alkyl group represented by R16 include one or more groups selected from substituent group γ.
Substituent group γ: a halogen atom, a cyano group, a carbamoyl group, a sulfo group, a guanidino group, an optionally protected amino group, an optionally protected hydroxyl group, an optionally protected carboxyl group, an optionally protected mercapto group, a C1-6 alkyl group, a C1-6 alkoxy group, an aryl group, a C1-6 alkylamino group, a di(C1-6alkyl)amino group, a C1-6 alkylthio group, a heterocyclic group, and an oxo group.
s number of R17 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6 alkyl group. R17 is preferably a hydrogen atom or a C1-6 alkyl group. Examples of the substituent for the C1-6 alkyl group represented by R17 include one or more groups selected from substituent group γ.
t number of R18 are the same or different and each represent a hydrogen atom, an optionally substituted C1-6 alkyl group, or an amino-protecting group. R18 is preferably a hydrogen atom or an amino-protecting group. Examples of the substituent for the C1-6 alkyl group represented by R18 include one or more groups selected from substituent group α.
t number of R19 are the same or different and each represent a hydrogen atom or an optionally substituted C1-6alkyl group. R19 is preferably a hydrogen atom or a C1-6 alkyl group. Examples of the substituent for the C1-6 alkyl group represented by R19 include one or more groups selected from substituent group γ.
s is an integer of 1 to 3. s is preferably 1 or 2.
t is an integer of 0 to 3. t is preferably 1 or 2.
q is an integer of 0 to 3. r is an integer of 0 to 3. r is preferably an integer of 1 to 3.
The compound represented by the formula (1) or the salt thereof of the present invention is preferably a compound or a salt thereof wherein A1 is a group having a polyaminopolycarboxylic acid structure; R1 is a hydrogen atom or an optionally substituted C1-6 alkyl group; R2 is a hydrogen atom or an optionally substituted C1-6 alkyl group; Z1, Z2, Z4, and Z5 are the same or different and each represent CR3b wherein R3b is as defined above; Z3 is CR3c wherein R3a is as defined above; L2 is an optionally substituted C1-6 alkylene group; L3 is an optionally substituted C1-6 alkylene group; and L1 is a group represented by the formula (3):
wherein R13, R14, R15, R16, q, and r are as defined above with the substituent for each group being the same as above.
The compound represented by the formula (1) or the salt thereof of the present invention is more preferably a compound or a salt thereof wherein A1 is a group represented by the formula (5), (6), (7), (8), (9), (10), (11), or (12):
wherein Ra, Rb, Rc, Rd, Re, Rf, Rg, Rh, Ri, X1, X2, X3, X4, X4a, X5, X6, X7, X8, X8a, X9, X10, Y1, Y2, Y3, Y4, Y5, Y6, Y7, Y8, and Q1 are as defined above;
R1 is a hydrogen atom; R2 is a hydrogen atom; Z1, Z2, Z4, and Z5 are the same or different and each represent CR3d wherein R3d is as defined above; Z3 is CR3e wherein R3e is as defined above; L2 is an optionally substituted C1-6 alkylene group; L3 is an optionally substituted C1-6 alkylene group; and L1 is a group represented by the formula (3a):
wherein R16a and r1 are as defined above with the substituent for each group being the same as above.
The compound represented by the formula (1) or the salt thereof of the present invention is further preferably any of the following compounds or salts thereof described in Examples: 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 1, compound No. A8), 2,2′,2″-(10-(2-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 2, compound No. B2), 2,2′,2″-(10-((4R,7R,10R)-19-(4-(N-((s)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,11,16-pentaoxo-4,7,10-tris(sulfomethyl)-3,6,9,12,15-pentaazanonadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 3, compound No. C3), (S)-2,2′,2′-(10-(19-(4-(N-(1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,11,16-trioxo-6,9-dioxa-3,12,15-triazanonadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 4, compound No. D3), 2,2′,2″-(10-((S)-4-(4-aminobutyl)-22-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,14,19-tetraoxo-9,12-dioxa-3,6,15,18-tetraazadocosyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 5, compound No. E3), (S)-2,2′,2″-(10-(28-(4-(N-(1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,11,20,25-tetraoxo-6,9,15,18-tetraoxa-3,12,21,24-tetraazaoctacosyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 6, compound No. F3), 2,2′,2″-(10-((R)-22-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,14,19-tetraoxo-4-(sulfomethyl)-9,12-dioxa-3,6,15,18-tetraazadocosyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 7, compound No. G3), 2,2′,2″-(10-((9R)-18-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-4-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)carbamoyl)-2,7,10,15-tetraoxo-9-(sulfomethyl)-3,8,11,14-tetraazaoctadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 8, compound No. H9),
2,2′,2″-(10-((4R,7R)-16-((5-(2-carboxy-1-(5-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethoxy)-1H-indol-1-yl)ethyl)pyridin-3-yl)oxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 9, compound No. I21), 2,2′,2″-(10-((4R,7R)-16-((5-(2-carboxy-1-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)-1H-indol-1-yl)ethyl)pyridin-3-yl)oxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 10, compound No. J9), 2,2′,2′-(10-(2-(((R)-1-((2-(4-(4-(N—((R)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 11, compound No. K8), 2,2′,2″-(10-((4S,9R)-18(4-(N—((S)-2-(4-(2-(6-aminopyridin-2-yl)ethyl)benzamido)-1-carboxyethyl)sulfamoyl)-3,5-dimethylphenoxy)-4-(((R)-1-((2-(4-(4-(4-(N—((S)-2-(4-(2-(6-aminopyridin-2-yl)ethyl)benzamido)-1-carboxyethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)carbamoyl)-2,7,10,15-tetraoxo-9-(sulfomethyl)-3,8,11,14-tetraazaoctadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 12, compound No. L10), 2,2′,2″-(10-(2-(((R)-1-((2-(4-(4-(N—((S)-2-(4-(2-(6-aminopyridin-2-yl)ethyl)benzamido)-1-carboxyethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 13, compound No. M2), 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-2-(4-(2-(6-aminopyridin-2-yl)ethyl)benzamido)-1-carboxyethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 14, compound No. N3), 2,2′,2′-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 15, compound No. 010), 2,2′,2′-(10-(2-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 16, compound No. P2), 2,2′,2″-(10-((4R,7R)-16-(4-((S)-2-carboxy-1-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)-2-fluorophenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 17, compound No. 012), 2,2′-((1-(((S)-2-(bis(carboxymethyl)amino)-3-(4-(3-((R)-1-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-1-oxo-3-sulfopropan-2-yl)thioureido)phenyl) propyl) (carboxymethyl)amino) propan-2-yl)azanediyl)diacetic acid (Example 18, compound No. R3), (S)-2,2′,2″-(10-(2-((2-(4-(4-(N-(1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 19, compound No. S2)
2,2′,2″,2′″-(2-(4-(3-((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)thioureido)benzyl)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrayl)tetraacetic acid (Example 20, compound No. T2), 2,2′-((1-(((S)-2-(bis(carboxymethyl)amino)-3-(4-(3-((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)thioureido)phenyl) propyl)(carboxymethyl)amino)propan-2-yl)azanediyl)diacetic acid (Example 21, compound No. U1), 2,2′,2″-(10-(2-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(4-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)butanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 22, compound No. V8), 2,2′,2″-(10-(2-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(6-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl) hexanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-oxoethyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 23, compound No. W10), 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(5-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)thiophene-2-carboxamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 24, compound No. X9), 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzamido)ethyl)sulfamoyl)phenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid hexasodium salt (Example 25, compound No. Y13), 2,2′,2″-(10-((4R,7R)-16-(4-(N—((S)-1-carboxy-2-(4-(3-(pyridin-2-ylamino)propyl)benzamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)-2,5,8,13-tetraoxo-4,7-bis(sulfomethyl)-3,6,9,12-tetraazahexadecyl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (Example 26, compound No. Z8), 2,2′-(7-((R)-1-carboxy-4-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-4-oxobutyl)-1,4,7-triazonane-1,4-diyl)diacetic acid (Example 27, compound No. Aa7), and 5-(((R)-1-((2-(4-(4-(N—((S)-1-carboxy-2-(5-(5, 6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)ethyl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-1-oxo-3-sulfopropan-2-yl)amino)-2-(11-(carboxymethyl)-1,4,8,11-tetraazabicyclo[6.6.2]hexadecan-4-yl)-5-oxopentanoic acid (Example 28, compound Nos. Ab9-a and Ab9-b (Ab9-a and Ab9-b are stereoisomers)).
Next, methods for producing the compound represented by the formula (1) of the present invention will be described.
The compound represented by the formula (1) of the present invention is produced by the combination of methods known per se in the art and can be produced by, for example, production methods given below.
Production Method 1
wherein R represents an amino-protecting group; and R1, R2, RB, Z1, Z2, Z3, Z4, Z5, A1, L1, L2, and L3 are as defined above.
(1) The compound represented by the formula (S1a) can be produced by deprotecting a compound represented by the formula (S1). This reaction can be carried out by a method described in, for example, T. W. Greene et al., Protective Groups in Organic Synthesis, Vol. 4, p. 696-926, 2007, John Wiley & Sons, INC.
(2) The compound represented by the formula (S2) is a compound known as a bifunctional chelate. For example, as the compound represented by the formula (S2), DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid), TETA (1,4,8,11-tetraazacyclotetradecane-1,4,8,11-tetraacetic acid), DTPA (diethylenetriaminepentaacetic acid), and DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tri-tert-butyl ester and 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid tribenzyl ester) having a protected carboxyl group are known.
(2-1) When RB in the formula (S2) is a hydroxyl group, the compound represented by the formula (1) can be produced by reacting the compound represented by the formula (S1a) with the compound represented by the formula (S2) in the presence of a condensing agent and in the presence or absence of a base. This reaction can be carried out by a method described in, for example, Bioconjugate Chem., Vol. 3, Issue 2, 1992 or Chemical Reviews, Vol. 97, p. 2243, 1997.
A solvent used in this reaction is not particularly limited as long as the solvent does not influence the reaction. Examples thereof include ethers, esters, halogenated hydrocarbons, nitriles, amides, alcohols, and water. These solvents may be used as a mixture. Preferred examples of the solvent include amides. N,N-Dimethylformamide and N,N-dimethylacetamide are more preferred. The amount of the solvent used is not particularly limited and may be 1 to 1,000 times (v/w) the amount of the compound represented by the formula (S1a).
Examples of the base used, if desired, in this reaction include inorganic bases and organic bases. The amount of the base used may be 1 to 50 times, preferably 1 to 10 times the mol of the compound represented by the formula (S1a).
Examples of the condensing agent used in this reaction include: carbodiimides such as N,N′-dicyclohexylcarbodiimide and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide; carbonyls such as carbonyldiimidazole; acid azides such as diphenylphosphorylazide; acid cyanides such as diethylphosphorylcyanide; 2-ethoxy-1-ethoxycarbonyl-1,2-dihydroquinoline; ureas such as O-benzotriazol-1-yl-1,1,3,3-tetramethyluronium=hexafluorophosphate and O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium=hexafluorophosphate; and phosphonium salts such as benzotriazol-1-yloxy-trisdimethylaminophosphonium hexafluorophosphate and (benzotriazol-1-yloxy)tripyrrolidinophosphonium hexafluorophosphate. The condensation method can involve mixing the compound represented by the formula (S1a) and the compound represented by the formula (S2), followed by the addition of the condensing agent. Alternatively, the compound represented by the formula (S2) may be activated in advance with the condensing agent and then reacted with the compound represented by the formula (S1a). In addition, an active ester such as N-hydroxysuccinimide or pentafluorophenol can also be used.
The amount of the compound represented by the formula (S2) used is not particularly limited and may be 0.5 to 10 times (w/w) the amount of the compound represented by the formula (S1a). The reaction may be carried out at a temperature of −30 to 100° C., preferably 0 to 50° C. for 1 minute to 72 hours.
(2-2) When RB in the formula (S2) is a leaving group, the compound represented by the formula (1) can be produced by reacting the compound represented by the formula (S1a) with the compound represented by the formula (S2) in the presence of a base.
A solvent used in this reaction is not particularly limited as long as the solvent does not influence the reaction. Examples thereof include ethers, esters, halogenated hydrocarbons, nitriles, and amides. These solvents may be used as a mixture. The amount of the solvent used is not particularly limited and may be 1 to 1,000 times (v/w) the amount of the compound represented by the formula (S1a).
Examples of the base used in this reaction include inorganic bases and organic bases. The amount of the base used may be 1 to 50 times, preferably 1 to 10 times the mol of the compound represented by the formula (S1a).
The amount of the compound represented by the formula (82) used is not particularly limited and may be 0.5 to 10 times (w/w) the amount of the compound represented by the formula (S1a). The reaction may be carried out at a temperature of −30 to 100° C., preferably 0 to 50° C. for 1 minute to 72 hours.
Production Method 2
wherein B1, R1, R2, Z1, Z2, Z3, Z4, Z5, L1, L2, L3, and Q1 are as defined above.
For example, as the compound represented by the formula (S3), NCS-DOTA (2-(p-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid) and MXDTPA (2-(p-isothiocyanatobenzyl)-5(6)-methyl-diethylenetriaminepentaacetic acid) are known. The compound represented by the formula (1a) can be produced by reacting the compound represented by the formula (S1a) with the compound represented by the formula (S3).
A solvent used in this reaction is not particularly limited as long as the solvent does not influence the reaction. Examples thereof include ethers, esters, halogenated hydrocarbons, nitriles, amides, alcohols, and water. These solvents may be used as a mixture. Preferred examples of the solvent include amides. N,N-Dimethylformamide and N,N-dimethylacetamide are more preferred. The amount of the solvent used is not particularly limited and may be 1 to 1,000 times (v/w) the amount of the compound represented by the formula (S1a).
Examples of the base used in this reaction include inorganic bases and organic bases. The amount of the base used may be 1 to 50 times, preferably 1 to 10 times the mol of the compound represented by the formula (S1a).
The amount of the compound represented by the formula (S3) used is not particularly limited and may be 0.5 to 10 times (w/w) the amount of the compound represented by the formula (S1a). The reaction may be carried out at a temperature of −30 to 100° C., preferably 0 to 50° C. for 1 minute to 72 hours.
Production Method 3
wherein R1, RA, RB, Z1, Z2, Z3, Z4, Z5, L1, L2, L3, and A1 are as defined above.
(1) The compound represented by the formula (S4a) can be produced by deprotecting a compound represented by the formula (S4). This reaction can be carried out according to production method 1(1).
(2) The compound represented by the formula (1b) can be produced by reacting the compound represented by the formula (S4a) with a compound represented by the formula (S5). This reaction can be carried out according to production method 1(2).
Next, methods for producing starting materials will be described.
Production Method A
wherein Rc represents a leaving group; and R5, R6, R8, R9, R10, R11, R12, RA, RB, Z1, Z2, Z4, Z5, L3, L4, L5, and n are as defined above.
(1) For example, as the compound represented by the formula (S6), 4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzoic acid is known. For example, as the compound represented by the formula (S8), benzyl (2-aminoethyl)carbamate is known. The compound represented by the formula (S7) can be produced by reacting the compound represented by the formula (S6) with the compound represented by the formula (S8). This reaction can be carried out according to production method 1(2).
(2) The compound represented by the formula (S7a) can be produced by deprotecting the compound represented by the formula (S7). This reaction can be carried out according to production method 1(1).
(3) The compound represented by the formula (S5a) can be produced by reacting the compound represented by the formula (S7a) with a compound represented by the formula (S9) in the presence of a base. A solvent used in this reaction is not particularly limited as long as the solvent does not influence the reaction. Examples thereof include ethers, esters, halogenated hydrocarbons, nitriles, and amides. These solvents may be used as a mixture. Preferred examples of the solvent include halogenated hydrocarbons. Methylene chloride is more preferred. The amount of the solvent used is not particularly limited and may be 1 to 1,000 times (v/w) the amount of the compound represented by the formula (S7a).
Examples of the base used, if desired, in this reaction include inorganic bases and organic bases. The amount of the base used may be 1 to 50 times, preferably, 1 to 10 times the mol of the compound represented by the formula (S7a). The amount of the compound represented by the formula (S9) used is not particularly limited and may be 1 to 50 times, preferably 1 to 10 times the mol of the compound represented by the formula (S7a).
The reaction may be carried out at a temperature of −30 to 100° C., preferably 0 to 50° C. for 1 minute to 72 hours.
In this production method, the conversion of Z3 is described. In the same way as this method, Z1, Z2, Z4, or Z5 can also be converted.
Production Method B
wherein R2, R14, R15, R16, RA, RB, Z1, Z2, Z3, Z4, Z5, L2, L3, and q are as defined above.
(1) For example, as the compound represented by the formula (S11), benzyl (2-aminoethyl)carbamate is known. The compound represented by the formula (S10) can be produced by reacting the compound represented by the formula (S5) with the compound represented by the formula (S11). This reaction can be carried out according to production method 1(2).
(2) The compound represented by the formula (S10a) can be produced by deprotecting the compound represented by the formula (S10). This reaction can be carried out according to production method 1(1).
(3) For example, as the compound represented by the formula (S12), Fmoc-cysteic acid and Fmoc-8-amino-3,6-dioxaoctanoic acid are known. The compound represented by the formula (S1b) can be produced by reacting the compound represented by the formula (S10a) with the compound represented by the formula (S12). This reaction can be carried out according to production method 1(2).
Production Method C
wherein R1, R2, R4, R5, R6, R7, R8, R9, R10, R11, R12, RB, Z1, Z2, Z4, Z5, L2, L3, L4, L5, m, and n are as defined above.
For example, as the compound represented by the formula (S6), 4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzoic acid is known. For example, as the compound represented by the formula (S13), (S)-methyl 3-amino-2-(4-(4-((2-(((benzyloxy)carbonyl)amino)ethyl)amino)-4-oxobutoxy)-2,6-dimethylphenylsulfonamido)propanoate is known. The compound represented by the formula (S10b) can be produced by reacting the compound represented by the formula (S13) with the compound represented by the formula (S6). This reaction can be carried out according to production method 1(2).
In this production method, the conversion of Z3 is described. In the same way as this method, Z1, Z2, Z4, or Z5 can also be converted.
Production Method D
wherein RD represents a carboxyl-protecting group; and R4, R5, R6, R7, R8, R9, R10, R11, R12, RB, Z1, Z2, Z4, Z5, L3, L4, L5, m, and n are as defined above.
The compound represented by the formula (S5b) can be produced by reacting a compound represented by the formula (S14) with the compound represented by the formula (S6). This reaction can be carried out according to production method 1(2).
In this production method, the conversion of Z3 is described. In the same way as this method, Z1, Z2, Z4, or Z5 can also be converted.
Production Method E
wherein R14, R15, R16, RA, RB, A1, L2, and q are as defined above.
(1) The compound represented by the formula (S15) can be produced by reacting the compound represented by the formula (S11) with the compound represented by the formula (S12). This reaction can be carried out according to production method 1(2).
(2) The compound represented by the formula (S15a) can be produced by deprotecting the compound represented by the formula (S15). This reaction can be carried out according to production method 1(1).
(3) The compound represented by the formula (S4b) can be produced by reacting the compound represented by the formula (S15a) with the compound represented by the formula (S2). This reaction can be carried out according to production method 1(2).
Production Method F
wherein R14, R15, R16, RA, B1, L2, Q1, and q are as defined above.
The compound represented by the formula (S4c) can be produced by reacting the compound represented by the formula (S15a) with the compound represented by the formula (S3). This reaction can be carried out according to production method 2.
The compounds obtained by the production methods mentioned above can be converted to other compounds, for example, through a reaction known per se in the art such as condensation, addition, oxidation, reduction, dislocation, substitution, halogenation, dehydration, or hydrolysis or through an appropriate combination of these reactions.
The compounds obtained by the production methods mentioned above can be isolated and purified by an ordinary method such as extraction, crystallization, distillation, or column chromatography. Alternatively, the compounds obtained by the production methods mentioned above may be used directly in next reactions without being isolated.
The compounds obtained by the production methods mentioned above and their intermediates may have amino, hydroxyl, or carboxyl groups. In this case, the reactions can be carried out with their protective groups appropriately replaced. Also, two or more protective groups, if any, can each be deprotected selectively through a reaction known per se in the art.
The compounds used in the production methods mentioned above can also be used as salts, if these compounds can be in a form of salt. Examples of the salts include the same as those exemplified as the salt of the compound represented by the formula (1).
The compounds used in the production methods mentioned above may have isomers (e.g., optical isomers, geometric isomers, and tautomers). In this case, these isomers may be used. Alternatively, the compounds used in the production methods mentioned above may be any of solvates, hydrates, and crystals in various forms. In this case, these solvates, hydrates, and crystals in various forms can be used.
The complex of the compound represented by the formula (1) or the salt thereof with a metal can be produced, for example, as described below. The compound represented by the formula (1) or the salt thereof and a metal ion are mixed in the presence of a buffer solution to produce the complex. The buffer solution used in this reaction is not particularly limited as long as the buffer solution does not influence the reaction. Examples thereof include a sodium acetate buffer solution, an ammonium acetate buffer solution, a sodium citrate buffer solution, and an ammonium citrate buffer solution. The pH range of the buffer solution is preferably 3 to 6. The reaction temperature and the reaction time differ depending on the combination of the compound represented by the formula (1) or the salt thereof and a radioactive metal and may be 0 to 150° C. and 5 to 60 minutes. The complex obtained by this production method can be isolated and purified by an ordinary method such as extraction, crystallization, distillation, or column chromatography. When the metal is a radioactive metal, the complex can also be produced according to this production method. However, the radioactive metal emits radiation, and the radioactive metal is in a trace amount. In consideration of these factors, the following things must be noted. Unnecessary prolongation of the reaction time is not preferred because of the possibility of causing the decomposition of a compound by radiation. Usually, a labeled compound can be obtained at a radiochemical yield exceeding 80%. If a higher purity is necessary, the compound can be purified by a method such as preparative liquid chromatography, preparative TLC, dialysis, solid-phase extraction, and/or ultrafiltration. Also, a metal fluoride complex, which is a conjugate of a fluoride and a metal, can be regarded as a metal and reacted with the compound represented by the formula (1) or the salt thereof to produce the complex. This reaction can be carried out by a method described in, for example, JP-B-5388355. For suppressing decomposition by radiation, it is preferred to add an additive such as gentisic acid, ascorbic acid, benzyl alcohol, tocopherol, gallic acid, gallic acid ester, or α-thioglycerol.
The complex of the compound represented by the formula (1) or the salt thereof with a metal of the present invention has high accumulation and persistence in integrin-expressing cells and exhibits fast blood clearance. Therefore, the complex is useful as an agent for the diagnosis or treatment, etc., of a disease involving an integrin.
In the case of using the compound represented by the formula (1) or the salt thereof of the present invention as an agent for diagnosis or treatment, etc., the compound or the salt is preferably used as a metal complex. Examples of such a metal complex include the following complexes on a use basis.
Examples of the complex useful as an agent for nuclear magnetic resonance diagnosis or the like include complexes containing a metal ion exhibiting paramagnetism (e.g., a paramagnetic ion of a metal selected from the group consisting of Co, Mn, Cu, Cr, Ni, V, Au, Fe, Eu, Gd, Dy, Tb, Ho, and Er) as a metal component.
Examples of the complex useful as an agent for x-ray diagnosis or the like include complexes containing a metal ion absorbing x-rays (e.g., an ion of a metal selected from the group consisting of Re, Sm, Ho, Lu, Pm, Y, Bi, Pb, Os, Pd, Gd, La, Au, Yb, Dy, Cu, Rh, Ag, and Ir) as a metal component.
Examples of the complex useful as an agent for radiodiagnosis, radiotherapy, or the like include complexes containing a radioactive metal ion (e.g., an ion of a radioactive metal selected from the group consisting of 18F aluminum complex, 18F gallium complex, 18F indium complex, 18F lutetium complex, 18F thallium complex, 44Sc, 47Sc, 51Cr, 52mMn, 55Co, 57Co, 58Co, 52Fe, 59Fe, 60Co, 62Cu, 64Cu, 67Cu, 67Ga, 68Ga, 72As, 72Se, 73Se, 75Se, 76As, 82Rb, 82Sr, 85Sr, 89Sr, 89Zr, 86Y, 87Y, 90Tc, 99mTc, 103Ru, 103Pd, 105Rh, 109Pd, 111In, 114mIn, 171mSn, 111Ag, 113mIn, 140La, 149Pm, 149Tb, 152Tb, 155Tb, 161Tb, 153Sm, 159Gd, 165Dy, 166Dy, 166Ho, 165Er, 169Yb, 175Yb, 177Lu, 186Re, 188Re, 192Ir, 197Hg, 198Au, 199Au, 201Tl, 203Hg, 211At, 212Bi, 212Pb, 213Bi, 217Bi, 223Ra, 225Ac and 227Th) as a metal component.
The radioactive metal is preferably a cytotoxic radioactive metal for use in an agent for treatment or the like, and is preferably a noncytotoxic radioactive metal for use in an agent for diagnosis or the like.
Examples of the noncytotoxic radioactive metal for use in an agent for diagnosis or the like include gamma ray-emitting nuclides and positron-emitting nuclides (e.g., 18F aluminum complex, 18F gallium complex, 18F indium complex, 18F lutetium complex, 18F thallium complex, 99mTc, 111In, 113mIn, 114mIn, 67Ga, 68Ga, 82Rb, 86Y, 87Y, 152Tb, 155Tb, 201Tl, 51Cr, 52Fe, 57Co, 58Co, 60Co, 82Sr, 85Sr, 197Hg, 44Sc, 62Cu, 64Cu or 89Zr). 18F aluminum complex, 111In, 67Ga, 68Ga, 64Cu, or 89Zr is preferred from the viewpoint of half-life, radiation energy, and easy labeling reaction, etc.
Examples of the cytotoxic radioactive metal for use in an agent for treatment or the like include alpha ray-emitting nuclides and beta ray-emitting nuclides. Specific examples thereof include 90Y, 114mIn, 117Sn, 186Re, 188Re, 64Cu, 67Cu, 59Fe, 89Sr, 198Au, 203Hg, 212Pb, 165Dy, 103Ru, 149Tb, 161Tb, 212Bi, 166Ho, 165Er, 153Sm, 177Lu, 213Bi, 223Ra, 225Ac or 227Th. Among these radioactive metals, 64Cu, 67Cu, 90Y, 153Sm, 166Ho, 177Lu, or 225Ac is preferred from the viewpoint of half-life, radiation energy, easy labeling reaction, and stability of the complex.
The agent for diagnosis or treatment, etc., of the present invention may be provided by any of a method for providing an already labeled preparation containing the complex of the compound represented by the formula (1) or the salt thereof with a metal and a method for providing a kit preparation containing the compound represented by the formula (1) or the salt thereof. When the agent for diagnosis or treatment is provided as an already labeled preparation, the agent containing the already labeled complex can be used directly in administration. When the agent is provided as a kit preparation, the agent is labeled with a desired radioactive metal in clinical settings and then used in administration. The kit preparation is provided in the form of an aqueous solution or a freeze-dried preparation. Use of the kit preparation eliminates the need of a special purification step, and a reaction solution can be prepared just before use as a dosing solution by merely performing reaction by the addition of a radioactive metal obtained from a generator stocked regularly in clinical settings or a radioactive metal provided by a drug manufacturer aside from or in set with the kit preparation.
The agent for treatment, etc., of the present invention may be used in combination with another anticancer agent. Examples of such anticancer agent include alkylating agents, antimetabolites, microtubule inhibitors, anticancer antibiotics, topoisomerase inhibitors, platinum preparations, molecular targeting drugs, hormones, and biologics. Examples of the alkylating agents include nitrogen mustard anticancer agents such as cyclophosphamide, nitrosourea anticancer agents such as ranimustine, and dacarbazine. Examples of the antimetabolites include 5-FU, UFT, carmofur, capecitabine, tegafur, TS-1, gemcitabine, and cytarabine. Examples of the microtubule inhibitors include alkaloid anticancer agents such as vincristine, and taxane anticancer agents such as docetaxel and paclitaxel. Examples of the anticancer antibiotics include mitomycin C, doxorubicin, epirubicin, daunorubicin, and bleomycin. Examples of the topoisomerase inhibitors include irinotecan and nogitecan having a topoisomerase I inhibitory effect, and etoposide having a topoisomerase II inhibitory effect. Examples of the platinum preparations include cisplatin, Paraplatin, nedaplatin, and oxaliplatin. Examples of the molecular targeting drugs include trastuzumab, rituximab, imatinib, gefitinib, erlotinib, bevacizumab, cetuximab, panitumumab, bortezomib, sunitinib, sorafenib, crizotinib, and regorafenib. Examples of the hormones include dexamethasone, finasteride, and tamoxifen. Examples of the biologics include interferons α, β, and γ and interleukin 2.
The agent for treatment, etc., of the present invention may be used in combination with a cancer therapy and can be used in combination with surgical operation as well as radiotherapy (including gamma knife therapy, cyberknife therapy, boron neutron capture therapy, proton radiation therapy, and heavy particle radiotherapy), MR-guided focused ultrasound surgery, cryotherapy, radiofrequency ablation, percutaneous ethanol injection therapy, arterial embolization, or the like.
Examples of the disease targeted by the agent for diagnosis or treatment, etc., of the present invention include mammalian (including humans) diseases involving an integrin. Examples of the diseases involving an integrin include cancer, ischemic disease, thrombosis, myocardial infarction, arteriosclerosis, angina pectoris, inflammation, osteolysis, osteoporosis, diabetic retinopathy, macular degeneration, myopia, ocular histoplasmosis, rheumatoid arthritis, osteoarthropathy, rubeotic glaucoma, ulcerative colitis, Crohn's disease, multiple sclerosis, psoriasis, and restenosis. The type of the cancer is not particularly limited. Examples thereof include rectal cancer, colon cancer, large intestine cancer, familial polyposis colorectal cancer, hereditary non-polyposis colorectal cancer, esophageal cancer, oral cancer, lip cancer, laryngeal cancer, hypopharyngeal cancer, tongue cancer, salivary gland cancer, stomach cancer, adenocarcinoma, medullary thyroid cancer, papillary thyroid carcinoma, kidney cancer, renalparenchyma cancer, ovary cancer, neck cancer, uterine body cancer, endometrial cancer, choriocarcinoma, pancreatic cancer, prostate cancer, testis cancer, breast cancer, ureteral cancer, skin cancer, melanoma, brain tumor, glioblastoma, astrocytoma, meningioma, medulloblastoma, peripheral neuroectodermal tumor, Hodgkin's lymphoma, non-Hodgkin's lymphoma, Burkitt's lymphoma, acute lymphatic leukemia (ALL), chronic lymphatic leukemia (CLL), acute myeloid leukemia (AML), chronic myeloid leukemia (CML), adult T-cell leukemia, hepatocellular cancer, gallbladder cancer, bile duct cancer, biliary cancer, bronchial cancer, lung cancer (small-cell lung cancer, non-small cell lung cancer, etc.), multiple myeloma, basalioma, teratoma, retinoblastoma, neuroblastoma, choroidal melanoma, seminoma, rhabdomyosarcoma, craniopharyngioma, osteosarcoma, chondrosarcoma, myosarcoma, liposarcoma, fibrosarcoma, Ewing sarcoma, and plasmacytoma. The agent for diagnosis or treatment, etc., of the present invention is preferably used for the suppression of solid cancer, preferably head and neck cancer, colorectal cancer, breast cancer, small-cell lung cancer, non-small cell lung cancer, glioblastoma, malignant melanoma, pancreatic cancer, or prostate cancer.
The agent for treatment, etc., of the present invention can be used for suppressing cancer by administering an effective amount thereof to a mammal including a human. In the case of using the agent as an anticancer agent, its effect has the broadest sense including both of a prophylactic effect of preventing, for example, cancer occurrence, metastasis or implantation, or recurrence, and a therapeutic effect of inhibiting cancer progression or ameliorating symptoms by suppressing the growth of cancer cells or reducing the size of tumor, and should not be interpreted restrictively in any case.
For pharmaceutical use of the complex of the compound represented by the formula (1) or the salt thereof with a metal of the present invention, usually, the complex may be appropriately mixed with a pharmacologically acceptable additive. Examples of the additive include excipients, disintegrants, binders, lubricants, corrigents, colorants, flavoring agents, surfactants, coating agents, stabilizers, and plasticizers. Examples of the excipients include: sugar alcohols such as erythritol, mannitol, xylitol, and sorbitol; saccharides such as saccharose, powder sugar, lactose, and glucose; cyclodextrins such as α-cyclodextrin, β-cyclodextrin, γ-cyclodextrin, hydroxypropyl β-cyclodextrin, and sulfobutyl ether β-cyclodextrin sodium; celluloses such as crystalline cellulose and microcrystalline cellulose; and starches such as corn starch, potato starch, and pregelatinized starch. Examples of the disintegrants include carmellose, carmellose calcium, croscarmellose sodium, carboxymethyl starch sodium, crospovidone, low-substituted hydroxypropylcellulose, and partly pregelatinized starch. Examples of the binders include hydroxypropylcellulose, carmellose sodium, and methylcellulose. Examples of the lubricants include stearic acid, magnesium stearate, calcium stearate, talc, hydrated silicon dioxide, light anhydrous silicic acid, and sucrose fatty acid ester. Examples of the corrigents include aspartame, saccharine, stevia, thaumatin, and acesulfame potassium. Examples of the colorants include titanium dioxide, red ferric oxide, yellow ferric oxide, black iron oxide, Food Red No. 102, Food Yellow No. 4, and Food Yellow No. 5. Examples of the flavoring agents include: essential oils such as orange oil, lemon oil, peppermint oil, and pine oil; extracts such as orange extract and peppermint extract; flavors such as cherry flavor, vanilla flavor, and fruit flavor; powder flavors such as apple micron, banana micron, peach micron, strawberry micron, and orange micron; vanillin; and ethylvanillin. Examples of the surfactants include sodium lauryl sulfate, sodium dioctyl sulfosuccinate, polysorbate, and polyoxyethylene hydrogenated castor oil. Examples of the coating agents include hydroxypropylmethylcellulose, aminoalkyl methacrylate copolymer E, aminoalkyl methacrylate copolymer RS, ethylcellulose, cellulose acetate phthalate, hydroxypropylmethylcellulose phthalate, methacrylic acid copolymer L, methacrylic acid copolymer LD, and methacrylic acid copolymer S. Examples of the stabilizers include gentisic acid, ascorbic acid, benzyl alcohol, tocopherol, gallic acid, gallic acid ester, and α-thioglycerol. Examples of the plasticizers include triethyl citrate, macrogol, triacetin, and propylene glycol. Any one of these additives or two or more thereof in combination may be used. These additives are not particularly limited by their contents and can be appropriately contained so as to adequately exert their effects according to each purpose. Such a preparation can be administered orally or parenterally in a form such as a tablet, a capsule, powders, a syrup, granules, a pill, a suspension, an emulsion, a solution, a powder preparation, a suppository, eye drops, nasal drops, eardrops, a patch, an ointment, or an injection according to a routine method. The administration, the dose, and the number of doses can be appropriately selected according to the age, body weight, and symptoms of a patient. Usually, the preparation can be administered orally or parenterally (e.g., through injection, through an intravenous drip, and by administration to a rectal site) to an adult.
In the case of using the radioactive metal, examples of its type can include alpha ray-emitting nuclides, beta ray-emitting nuclides, gamma ray-emitting nuclides, and positron-emitting nuclides. A beta ray-emitting nuclide (i.e., a nuclide which emits R rays) is preferred for the agent for treatment, etc.
The agent for diagnosis, etc., of the present invention can be used in the imaging of integrin expression. In the presence of a tumor or a neovessel expressing the integrin protein in the body, the complex of the compound represented by the formula (1) or the salt thereof with a metal of the present invention accumulates in the tumor or the like. Thus, the tumor can be imaged by the detection of radiation using an instrument such as a single photon emission computed tomography (SPECT) apparatus, a positron emission tomography (PET) apparatus, or a scintillation camera. Before treatment, integrin expression or the presence or absence of abnormal integrin accumulation at normal tissues is confirmed by the administration of the diagnostic drug. As a result, the applicability of a therapeutic drug can be determined, or the therapeutic drug can be presumed to be more effective for the imaged tumor having higher accumulation. Also, the complex of the compound represented by the formula (1) or the salt thereof with a metal of the present invention can be used in the determination of a therapeutic effect. The diagnostic drug of the present invention is administered to a patient who has received the therapeutic drug of the present invention or any of other treatments so that a tumor is imaged. Decrease or increase in tumor size can be determined by observing change in accumulation over time.
The dose of the agent for treatment, etc., of the present invention differs depending on the age, sex, and symptoms of a patient, an administration route, the number of doses, and a dosage form. In general, the dose of the pharmaceutical composition can be selected, for example, within the range of 0.0000001 mg to 100 mg per kg of body weight for one dose, though the dose according to the present invention is not limited thereto. One dose in an adult can be 18.5 MBq to 7400 MBq in terms of the amount of radioactivity.
The dose of the agent for diagnosis, etc., of the present invention also differs depending on the age, sex, and symptoms of a patient, an administration route, the number of doses, and a dosage form. In general, the dose of the pharmaceutical composition can be selected, for example, within the range of 0.0000001 mg to 100 mg per kg of body weight for one dose, though the dose according to the present invention is not limited thereto. One dose in an adult can be 111 MBq to 740 MBq in terms of the amount of radioactivity.
Next, the present invention will be described in more detail with reference to Reference Examples, Examples, and Test Examples. However, the present invention is not intended to be limited by them.
The carrier used in silica gel column chromatography was silica gel 60N (spherical/neutral) 63 to 210 μm (Kanto Chemical Co., Inc.), unless otherwise specified. A mixing ratio for an eluent is a volume ratio. For example, “hexane/ethyl acetate=90/10 to 50/50” means that an eluent of “hexane:ethyl acetate=90:10” was changed to an eluent of “hexane:ethyl acetate=50:50”.
1H-NMR spectra were measured using tetramethylsilane as an internal standard and Bruker AV300 (Bruker Corp.) or JEOL JNM-AL400 model (JEOL Ltd.), and δ values were indicated by ppm.
For HPLC analysis, measurement was carried out using Nexera HPLC System (Shimadzu Corp.) (column: TSKgel ODS-100Z (Tosoh Corp.), solvent: solution A=0.1% formic acid/water, solution B=0.1% formic acid/methanol/acetonitrile (4:1), gradient cycle: 0.0 min (solution A/solution B=90/10), 30 min (solution A/solution B=0/100), 40 min (solution A/solution B=0/100), flow rate: 1.0 mL/min) or Waters 600E system (Waters Corp.) (column: SunFire C18OBD 4.6×150 mm (Waters Corp.) and CAPCELL PAK C18MG 4.6×150 mm (Shiseido Japan Co., Ltd.), gradient cycle: 0.0 min (solution A/solution B=80/20), 10 min (solution A/solution B=0/100), 15 min (solution A/solution B=0/100), flow rate: 1.0 mL/min), unless otherwise specified. If different analysis conditions were used for optical isomer separation or the like, the conditions were described in Examples. Preparative HPLC was carried out using Waters 600E system (Waters Corp.) (column: SunFire Prep C18 OBD 30×150 mm (Waters Corp.) or SunFire Prep C18 OBD 19×150 mm (Waters Corp.), solvent: solution A=0.11 formic acid/water, solution B=0.1% formic acid/methanol:acetonitrile (4:1) or solvent: solution A=10 mmol/L aqueous ammonium acetate solution, solution B=10 mmol/L ammonium acetate/methanol:acetonitrile (4:1)), unless otherwise specified. TLC analysis was conducted using silica gel 60F254 (Merck KGaA) or RP-18F254 (Merck KGaA), unless otherwise specified.
For MS and LC/MS analysis, measurement was carried out using LCMS-2010EV (Shimadzu Corp.) (column: SunFire C18 4.6×150 mm (Waters Corp.), solvent: solution A=0.1% formic acid/water, solution B=0.1% formic acid/methanol:acetonitrile (4:1), gradient cycle: 0.0 min (solution A/solution B=80/20), 10.0 min (solution A/solution B=0/100), 15.0 min (solution A/solution B=0/100), flow rate: 1 mL/min) or ACQUITY SQD LC/MS System (Waters Corp.) (column: BEHC18 2.1×30 mm (Waters Corp.), solution A=0.1% formic acid/water, solution B=0.1% formic acid/acetonitrile, gradient cycle: 0.0 min (solution A/solution B=0.95/5), 2.0 min (solution A/solution B=5/95), 3.0 min (solution A/solution B=5/95), flow rate: 0.5 mL/min). Retention time (min) was indicated by rt (min), and ESI positive and negative ion peaks were detected. The MS spectra of some high-molecular-weight compounds were measured using Q-TOF Premier (Waters Corp.).
Each abbreviation has the following meaning: Bn: benzyl, Boc: tert-butoxycarbonyl, tBu: tert-butyl, DIEA: N,N-diisopropylethylamine, DMAc: N,N-dimethylacetamide, DMAP: 4-dimethylaminopyridine, DMF: N, N-dimethylformamide, DMFDA: N,N-dimethylformamide dimethyl acetal, DMSO: dimethyl sulfoxide, Et: ethyl, Fmoc: 9-fluorenylmethyloxycarbonyl, HATU: O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate, HBTU: O-benzotriazol-1-yl 1,1,3,3-tetramethyluronium hexafluorophosphate, NMP: N-methylpyrrolidone, TBS: tert-butyldimethylsilyl, TFA: trifluoroacetic acid, THF: tetrahydrofuran, and Z: benzyloxycarbonyl
Compound (A1) was obtained according to the method described in Journal of Medicinal Chemistry, 2000, Vol. 43, p. 3736-3745.
Compound (A2) was obtained according to the method described in Bioconjugate chemistry, 2006, Vol. 17, p. 1294-1313.
(1) To a solution of compound (A2) (130 mg), compound (A1) (57.0 mg), and DIEA (250 μL) in DMF (2 mL), a solution of HBTU (85.5 mg) in DMF (0.5 mL) was added, and the mixture was stirred at room temperature for 1 hour. Water (500 μL) and acetonitrile (2 mL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (A3) (152 mg). LC/MS (SunFire) rt (min): 9.43 MS (ESI, m/z): 829.10 [M+H]+, 827.15 [M−H]−
(2) Compound (A3) (27.8 mg), methanol (10 mL), and 10% Pd/C (10 mg) were placed in a sealed tube and stirred for 3 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (A4) (20.8 mg). LC/MS (SunFire) rt (min): 6.09 MS (ESI, m/z): 695.10 [M+H]+, 693.10 [M−H]−
(3) To a solution of compound (A4) (58.5 mg), Fmoc-cysteic acid (65.9 mg), and DIEA (100 μL) in DMF (0.8 mL), a solution of HBTU (63.7 mg) in DMF (0.3 mL) was added, and the mixture was stirred at room temperature for 1 hour. Water (100 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (A5) (43.4 mg). LC/MS (ACQUITY) rt (min): 1.32 MS (ESI, m/z): 1068.6 [M+H]+, 1066.6 [M−H]−
(4) To a solution of compound (A5) (26.5 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. To the obtained oil, Fmoc-cysteic acid (19.4 mg), DMF (0.6 mL), and DIEA (100 μL) were added, then, a solution of HBTU (18.8 mg) in DMF (150 μL) was added, and the mixture was stirred at room temperature for 70 minutes. Water (100 μL) was added thereto, and the solvent was distilled off under reduced pressure. Then, DMF (0.5 mL) and diethylamine (0.5 mL) were added to the residue, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and then, insoluble matter was filtered off. A 50% aqueous acetonitrile solution (300 μL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (A6) (15.6 mg). LC/MS (SunFire) rt (min): 9.56 MS (ESI, m/z): 997.15 [M+H]+, 995.20 [M−H]−
(5) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (14.0 mg) and DIEA (50 μL) in DMF (200 μL), a solution of HBTU (9.0 mg) in DMF (100 μL) was added, and the mixture was stirred at room temperature for 5 minutes. Then, the reaction mixture was added to a solution of compound (A6) (15.6 mg) and DIEA (10 μL) in DMF (200 μL), and the mixture was stirred at room temperature for 1 hour. Water (100 μL) and acetonitrile (100 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (A7) (10.5 mg). LC/MS (SunFire) rt (min): 10.74 MS (ESI, m/z): 685.15 [M+2H]2+
(6) A mixture of compound (A7) (5.4 mg), THF (450 μL), water (100 μL), and a 3 mol/L aqueous lithium hydroxide solution (100 μL) was stirred at room temperature for 75 minutes. TFA was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred for 1.5 hours. Then, the solvent was distilled off under reduced pressure. To the obtained residue, a 50% aqueous acetonitrile solution (200 μL) and TFA (10 μL) were added, and the mixture was purified by preparative HPLC to obtain compound (A8) (4.2 mg). LC/MS (SunFire) rt (min): 10.74 MS (ESI, m/z): 685.15 [M+2H]2+
(1) To a solution of compound (A5) (8.8 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 150 minutes. The solvent was distilled off under reduced pressure. To the residue, DMF (0.2 mL) and DIEA (10 μL) were added, then a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (7.1 mg), DMF (0.1 mL), DIEA (20 μL), and HBTU (4.5 mg) in DMF (45 μL) was added, and the mixture was stirred at room temperature for 3 hours. Tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (7.0 mg), DMF (50 μL), DIEA (20 μL), HBTU (4.5 mg), and DMF (50 μL) were added thereto, and the mixture was stirred for 30 minutes. Water (100 μL) and a 50% aqueous acetonitrile solution (400 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (B1) (10.0 mg). LC/MS (SunFire) rt (min): 8.89 MS (ESI, m/z): 701.25 [M+2H]2+, 1399.20 [M−H]−
(2) To compound (E1) (2.4 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (100 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 1 hour. TFA was distilled off under reduced pressure. A 20% aqueous acetonitrile solution (1.2 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (B2) (1.8 mg). LC/MS (SunFire) rt (min): 8.14 MS (ESI, m/z): 609.90 [M+2H]2+, 1217.05 [M−H]−
(1) To compound (A6) (21.5 mg), Fmoc-cysteic acid (21.1 mg), DMF (0.8 mL), and DIEA (30 μL) were added, then a solution of HBTU (19.7 mg) in DMF (200 μL) was added, and the mixture was stirred at room temperature for 70 minutes. Water (200 μL) and diethylamine (0.5 mL) were added thereto, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and then, a 50% aqueous acetonitrile solution (0.6 mL) was added to the residue. Insoluble matter was filtered off, and the residue was purified by preparative HPLC to obtain compound (C1) (15.0 mg). LC/MS (ACQUITY) rt (min): 0.91 MS (ESI, m/z): 1148.4 [M+H]+, 1146.4 [M−H]−
(2) To compound (C1) (7.2 mg), a 50% aqueous methanol solution (300 μL) and a 4 mol/L solution of hydrogen chloride in dioxane (20 μL) were added, and the solvent was distilled off under reduced pressure. To the obtained residue, tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (12.6 mg), DMF (400 μL), and DIEA (20 μL) were added, then a solution of HBTU (7.2 mg) in DMF (200 μL) was added, and the mixture was stirred at room temperature for 1 hour. HBTU (10.0 mg) was added thereto, and the mixture was stirred for 1 hour, followed by the addition of water (300 μL). The mixture was purified by preparative HPLC to obtain compound (C2) (4.1 mg). LC/MS (ACQUITY) rt (min): 1.16 MS (ESI, m/z): 852.3 [M+2H]2+, 850.3[M−2H]2−
(3) A mixture of compound (C2) (4.1 mg), THF (1.3 mL), water (150 μL), and a 3 mol/L aqueous lithium hydroxide solution (150 μL) was stirred at room temperature for 140 minutes. TFA was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred for 2 hours. Then, TFA was distilled off under reduced pressure. A 10 mmol/L aqueous ammonium acetate solution (800 μL) was added to the residue, and then, the mixture was purified by preparative HPLC to obtain compound (C3) (2.3 mg). LC/MS (ACQUITY) rt (min): 0.93 MS (ESI, m/z): 759.7 [M−2H]2−
(1) To a solution of Fmoc-8-amino-3,6-dioxaoctanoic acid (31.0 mg), compound (A4) (20.8 mg), and DIEA (50 μL) in DMF (400 μL), a solution of HBTU (22.7 mg) in DMF (100 μL) was added, and the mixture was stirred at room temperature for 1 hour. Water (100 μL) and acetonitrile (100 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (D1) (22.8 mg). LC/MS (SunFire) rt (min): 9.81 MS (ESI, m/z): 531.95 [M+2H]2+
(2) To a solution of compound (D1) (7.5 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1 hour. Then, the solvent was distilled off under reduced pressure. To the obtained residue, tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (8.1 mg), DMF (200 μL), and DIEA (40 μL) were added, then a solution of HBTU (5.3 mg) in DMF (100 μL) was added, and the mixture was stirred at room temperature for 1 hour. Water (100 μL) and acetonitrile (200 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain a fraction containing compound (D2). LC/MS (SunFire) rt (min): 8.36 MS (ESI, m/z): 698.10 [M+2H]2+, 1392.50 [M−H]−
(3) The solvent in the fraction containing compound (D2) obtained in the step (2) was distilled off under reduced pressure. Then, THF (350 μL), water (50 μL), and a 3 mol/L aqueous lithium hydroxide solution (50 μL) were added to the residue, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred for 1.5 hours. The solvent was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (600 μL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (D3) (1.8 mg). LC/MS (SunFire) rt (min): 7.66 MS (ESI, m/z): 606.85 [M+2H]2+, 404.95 [M+3H]3+
(1) To a solution of compound (D1) (29.8 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 2.5 hours. Then, the solvent was distilled off under reduced pressure. To the obtained residue, DMF (0.4 mL) and DIEA (10 μL) were added, then a solution of Fmoc-Lys(BOC)-OH (39.4 mg), DMF (150 μL), DIEA (20 μL), and HBTU (26.5 mg) in DMF (150 μL) was added, and the mixture was stirred at room temperature for 1 hour. Water (100 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (E1) (4.4 mg). LC/MS (SunFire) rt (min): 10.56 MS (ESI, m/z): 645.85 [M+2H]2+, 430.35 [M+3H]3+, 1288.45 [M−H]−
(2) To a solution of compound (E1) (4.4 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 8 hours. The solvent was distilled off under reduced pressure. DMF (0.4 mL) and DIEA (10 μL) were added to the residue, and the mixture was stirred. Then, a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (7.8 mg), DMF (0.1 mL), DIEA (10 μL), and HBTU (5.2 mg) in DMF (100 μL) was added thereto, and the mixture was stirred at room temperature for 1 hour. Water (100 μL) was added thereto, and then, the mixture was purified by preparative HPLC to obtain compound (E2) (4.4 mg). LC/MS (SunFire) rt (min): 8.21 MS (ESI, m/z): 812.35 [M+2H]2+
(3) To compound (E2) (4.4 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (0.1 mL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 1.5 hours. The solvent was distilled off under reduced pressure. A 20% aqueous acetonitrile solution (1 mL) and methanol (0.6 mL) were added to the residue, and the mixture was purified by preparative HPLC to obtain compound (E3) (2.5 mg). LC/MS (SunFire) rt (min): 6.19 MS (ESI, m/z): 670.75 [M+2H]2+, 447.60 [M+3H]3+
(1) To a solution of compound (D1) (30.9 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 50 minutes. The solvent was distilled off under reduced pressure. To the residue, DMF (0.3 mL) and DIEA (15 μL) were added, then a solution of Fmoc-8-amino-3,6-dioxaoctanoic acid (23.0 mg), DMF (150 μL), DIEA (15 μL), and HBTU (22.0 mg) in DMF (150 μL) was added, and the mixture was stirred at room temperature 50 minutes. Water (100 μL) was added thereto, and then, the mixture was purified by preparative HPLC to obtain compound (F1) (10.9 mg). LC/MS (ACQUITY) rt (min): 1.29 MS (ESI, m/z): 1207.7 [M+H]+, 604.7 [M+2H]2+, 1205.7 [M−H]−
(2) To a solution of compound (F1) (10.9 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. To the residue, DMF (0.4 mL) and DIEA (15 μL) were added, then a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (15.5 mg), DMF (100 μL), DIEA (15 μL), and HBTU (9.6 mg) in DMF (100 μL) was added, and the mixture was stirred at room temperature for 30 minutes. Water (100 μL) was added thereto, and the mixture was purified by preparative HPLC. The solvent was distilled off under reduced pressure to obtain a fraction containing compound (F2) (12.4 mg). LC/MS (SunFire) rt (min): 7.80 MS (ESI, m/z): 514.10 [M+3H]3+, 1537.80 [M−H]−
(3) To compound (F2) (10.4 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (0.1 mL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 80 minutes. TFA was distilled off under reduced pressure. A 20% aqueous acetonitrile solution (2.1 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (F3) (4.0 mg). LC/MS (SunFire) rt (min): 7.17 MS (ESI, m/z): 679.45 [M+2H]2+, 453.35 [M+3H]3+
(1) To a solution of compound (D1) (27.3 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1 hour. Then, the solvent was distilled off under reduced pressure. To the obtained residue, Fmoc-cysteic acid (20.1 mg), DMF (0.7 mL), and DIEA (20 μL) were added, then a solution of HBTU (19.5 mg) in DMF (200 μL) was added, and the mixture was stirred at room temperature for 10 minutes. Water (0.5 mL) and ethyl acetate (2 mL) were added to the reaction mixture. The aqueous layer was separated and purified by preparative HPLC to obtain compound (G1) (11.2 mg). LC/MS (SunFire) rt (min): 11.78 MS (ESI, m/z): 1235.35 [M+Na]+, 629.40 [M+2Na]2+, 1212.40 [M−H]−
(2) To a solution of compound (G1) (11.2 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. The solvent was distilled off under reduced pressure. To the obtained residue, DMF (0.2 mL) and DIEA (10 μL) were added, and the mixture was stirred. A solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (15.5 mg), DMF (200 μL), DIEA (10 μL), and HBTU (10.5 mg) in DMF (100 μL) was added to the reaction mixture, and the mixture was stirred at room temperature for 30 minutes. To the reaction mixture, water (100 μL) was added, then a 50% aqueous acetonitrile solution (1.2 mL) was added, and then, the mixture was purified by preparative HPLC to obtain compound (G2) (7.6 mg). LC/MS (SunFire) rt (min): 8.26 MS (ESI, m/z): 1545.80 [M+H]+, 773.90 [M+2H]2+, 1543.85 [M−H]−
(3) To compound (G2) (7.6 mg), THF (1 mL), water (140 μL), and a 3 mol/L aqueous lithium hydroxide solution (140 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 100 minutes. TFA was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (1.2 mL) and water (500 μL) were added to the residue, and the mixture was purified by preparative HPLC to obtain compound (G3) (5.1 mg). LC/MS (SunFire) rt (min): 8.30 MS (ESI, m/z): 682.50 [M+2H]2+
(1) To a mixture of L-glutamic acid dibenzyl ester hydrochloride (86.9 mg), tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (114 mg), DMF (2 mL), and DIEA (100 μL), HBTU (83 mg) was added, and the resulting mixture was stirred at room temperature for 30 minutes. The solvent was distilled off under reduced pressure, and ethyl acetate (5 mL) and a saturated aqueous solution of sodium chloride (3 mL) were added to the residue. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (5 mL) five times. The combined organic layers were dried over anhydrous magnesium sulfate and purified by silica gel column chromatography (ethyl acetate) to obtain compound (H1) (96 mg). TLC Rf: 0.58 (ethyl acetate/methanol=5/1) MS (ESI, m/z): 904.8 [M+Na]+
(2) Compound (H1) (90.0 mg), methanol (10 mL), and 10% Pd/C (50 mg) were placed in a sealed tube and stirred for 3 hours in a 0.4 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (H2) (80 mg). LC/MS (SunFire) rt (min): 9.50 MS (ESI, m/z): 702.20 [M+H]+, 700.35 [M−H]−
(3) A mixture of ethyl 4-(2-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)ethyl)benzoate (2.76 g), THF (70 mL), DIEA (4.7 mL), and di-tert-butyl dicarbonate (4.1 mL) was refluxed for 19 hours. Di-tert-butyl dicarbonate (4 mL) and DIEA (5 mL) were added thereto, and the mixture was refluxed for 6 hours. The solvent was distilled off under reduced pressure. The obtained residue was dissolved in ethyl acetate (200 mL), and the solution was washed with water and a saturated aqueous solution of sodium chloride. The obtained product was purified by silica gel column chromatography (hexane/ethyl acetate=5/1 to 2/1) to obtain compound (H3) (1.47 g). MS (ESI, m/z): 411.43 [M+H]+
(4) To a solution of compound (H3) (213 mg) in THF (15 mL) and methanol (5 mL), a 2 mol/L aqueous lithium hydroxide solution (3 mL) was added, and the mixture was stirred at room temperature for 1 hour and then left overnight. Water (10 mL) was added thereto, and the mixture was adjusted to pH 4 by the addition of sodium bisulfate, followed by extraction with ethyl acetate (20 mL) three times. The extract was washed three times with a saturated aqueous solution of sodium chloride (30 mL) and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (H4) (202 mg). MS (ESI, m/z): 383.3 [M+H]+, 381.4 [M−H]−
(5) To a solution of compound (A2) (266 mg) and compound (H4) (150 mg) in DMF (5 mL) and DIEA (0.6 mL), HBTU (178 mg) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and ethyl acetate (10 mL) and a saturated aqueous solution of sodium bicarbonate (10 mL) were added to the residue, followed by extraction with ethyl acetate (20 mL) twice. The combined organic layers were washed twice with a saturated aqueous solution of sodium chloride (20 mL), then dried over anhydrous sodium sulfate, and purified by silica gel column chromatography (ethyl acetate/methanol=40/1) to obtain compound (H5) (187 mg). LC/MS (SunFire) rt (min): 12.25 MS (ESI, m/z): 929.25 [M+H]+, 927.25 [M−H]−
(6) Compound (H5) (180 mg), methanol (10 mL), and 10% Pd/C (50 mg) were placed in a sealed tube and stirred for 5 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (H6) (131 mg). MS (ESI, m/z): 795.7 [M+H]+, 695.5 [M−BOC]+, 793.2 [M−H]−
(7) To a solution of compound (H6) (130 mg) and Fmoc-cysteic acid (77.0 mg) in DMF (4 mL) and DIEA (200 μL), HBTU (68.4 mg) was added, and the mixture was stirred at room temperature for 1 hour. A saturated aqueous solution of sodium chloride (5 mL), ethyl acetate (5 mL), and water (5 mL) were added thereto. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (10 mL) six times. The organic layers were combined, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=5/1) to obtain compound (H7) (66.6 mg). LC/MS (SunFire) gradient cycle: 0.0 min (solution A/solution B=30/70), 10.0 min (solution A/solution B=0/100), 15.0 min (solution A/solution B=0/100) rt (min): 11.55 MS (ESI, m/z): 1166.40 [M−H]−
(8) To a solution of compound (H7) (55 mg) in DMF (4 mL), diethylamine (2 mL) was added, and the mixture was stirred at room temperature for 150 minutes. The solvent was distilled off under reduced pressure. To the obtained residue, compound (H2) (11.0 mg), DMF (0.5 mL), and DIEA (50 μL) were added, then HBTU (15.1 mg) was added, and the mixture was stirred at room temperature for 20 minutes. DIEA (20 μL) was added thereto, and the mixture was stirred at room temperature for 2 hours. The solvent was distilled off under reduced pressure. The obtained residue was purified by preparative HPLC to obtain compound (H8) (3.2 mg). LC/MS (SunFire) gradient cycle: 0.0 min (solution A/solution B=60/40), 30.0 min (solution A/solution B=0/100) rt (min): 17.12 MS (ESI, m/z): 853.45 [M+3H]3+, 819.85 [M+3H-BOC]3+, 786.70 [M+3H-2BOC]3+
(9) A mixture of compound (H8) (3.2 mg), THF (350 μL), water (50 μL), and a 3 mol/L aqueous lithium hydroxide solution (35 μL) was stirred at room temperature for 90 minutes. TFA was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred for 90 minutes. Then, TFA was distilled off under reduced pressure. To the obtained residue, a 50% aqueous acetonitrile solution (800 μL) was added, and the mixture was purified by preparative HPLC to obtain compound (H9) (2.7 mg). LC/MS (SunFire) rt (min): 10.25 MS (ESI, m/z): 721.30 [M+3H]3+, 1078.80 [M−2H]2−
(1) To a suspension of 1,4,7,10-tetraazacyclododecane (5.0 g), sodium acetate trihydrate (13.0 g), and DMAc (40 mL), a solution of benzyl bromoacetate (22 g) in DMAc (20 mL) was added dropwise at 20° C. or lower over 20 minutes, and then, the mixture was stirred at room temperature for 20 hours. Ethyl acetate (500 mL) was added to the reaction mixture, and the mixture was washed three times with water (300 mL), then dried over anhydrous sodium sulfate, and purified by silica gel column chromatography (ethyl acetate/methanol=5/1 to 1/1) to obtain compound (I11) (2.0 g). TLC Rf: 0.07 (ethyl acetate/methanol=5/1)
(2) To a mixture of compound (I11) (0.650 g), acetonitrile (8 mL), and potassium carbonate (160 mg), tert-butyl bromoacetate (156 μL) was added, and the resulting mixture was stirred at room temperature for 24 hours. Ethyl acetate (100 mL) and a saturated aqueous solution of sodium bicarbonate (50 mL) were added thereto. The organic layer was separated, then washed with a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=3/1) to obtain compound (I2) (319 mg). TLC Rf: 0.48 (acetonitrile/water=9/1) MS (ESI, m/z): 753.5 [M+Na]+
(3) To compound (I2) (130 mg), a 4 mol/L solution of hydrogen chloride in dioxane (4 mL) was added, and the mixture was stirred at room temperature for 22 hours. The solvent was distilled off under reduced pressure. To the obtained residue, a 50% aqueous acetonitrile solution (2 mL) was added, and the mixture was purified by preparative HPLC to obtain compound (I3) (39.2 mg). LC/MS (SunFire) rt (min): 9.44 MS (ESI, m/z): 675.10 [M+H]+, 673.25 [M−H]−
(4) To a mixture of 5-bromo-3-hydroxypyridine (2.98 g), potassium carbonate (3.75 g), and DMF (35 mL), ethyl 4-bromobutanoate (3.9 mL) was added, and the resulting mixture was stirred at 40° C. for 2 hours. A saturated aqueous solution of ammonium chloride (30 mL) and ethyl acetate (100 mL) were added to the reaction mixture. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (100 mL) twice. The organic layers were combined, then washed with a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=10/1 to 7/1) to obtain compound (I4) (4.13 g). 1H-NMR (400 MHz, CDCl3) δ: 8.28 (1H, brs), 8.22 (1H, brs), 7.35 (1H, dd, J=2.16, 2.24 Hz), 4.17 (2H, q, J=7.16 Hz), 4.06 (2H, t, J=6.12 Hz), 2.52 (2H, t, J=7.24 Hz), 2.13 (2H, tt, J=6.12, 7.24 Hz), 1.27 (3H, t, J=7.12 Hz)
(5) To a solution of compound (I4) (1.89 g) in methanol (20 mL) and THF (20 mL), a 5 mol/L aqueous sodium hydroxide solution (3 mL) was added, and the mixture was stirred at room temperature for 2 hours. Concentrated hydrochloric acid (2 mL) was added thereto, and the solvent was distilled off under reduced pressure. Then, water (50 mL) was added to the residue, followed by extraction with ethyl acetate (50 mL) four times. The organic layers were combined and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (I5) (1.76 g). TLC Rf: 0.19 (hexane/ethyl acetate=1/1) HPLC (SunFire) rt (min): 11.97 MS (ESI, m/z): 259.9 [M+H]+
(6) To a mixture of compound (I5) (1.76 g), Z-ethylenediamine hydrochloride (1.87 g), DMF (40 mL), and DIEA (2.8 mL), HBTU (2.64 g) was added, and the resulting mixture was stirred at room temperature for 2.5 hours. Ethyl acetate (250 mL) and water (100 mL) were added thereto. The organic layer was separated, then washed twice with water (300 mL) and once with a saturated aqueous solution of sodium chloride (300 mL), and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was recrystallized from ethyl acetate to obtain compound (I6) (1.73 g). TLC Rf: 0.58 (ethyl acetate) HPLC (SunFire) rt (min): 13.60
(7) To a mixture of compound (I6) (1.70 g), 3,3,3-triethoxy-1-propyne (1.1 g), acetonitrile (20 mL), triethylamine (25 mL), and DMF (20 mL), dichlorobis(triphenylphosphine)palladium(II) (250 mg) and copper(I) iodide (38 mg) were added, and the resulting mixture was stirred at 70° C. for 3 hours in a nitrogen atmosphere and then left all night and all day at room temperature. The solvent was distilled off under reduced pressure. The obtained residue was dissolved in ethyl acetate (100 mL), and the solution was washed three times with water (100 mL) and once with a saturated aqueous solution of sodium chloride (100 mL) and dried over anhydrous sodium sulfate. Then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=0/10 to 2/8) to obtain compound (I7) ((1.7 g). TLC Rf 0.26 (hexane/ethyl acetate=1/2) HPLC (SunFire) rt (min): 14.65 MS (ESI, m/z): 550.3 [M+Na]+
(8) To a solution of compound (I7) (1.70 g) in acetonitrile (25 mL), 2 mol/L hydrochloric acid (2 mL) was added, and the mixture was stirred at room temperature for 30 minutes. Ethyl acetate (100 mL) was added thereto, and the mixture was washed with a saturated aqueous solution of sodium bicarbonate and a saturated aqueous solution of sodium chloride, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (ethyl acetate/methanol=0/10 to 2/8) to obtain compound (I8) (1.3 g). TLC Rf: 0.26 (hexane/ethyl acetate=1/2) HPLC (SunFire) rt (min): 13.74 MS (ESI, m/z): 454.1 [M+H]+
(9) A mixture of 4-((tert-butyldimethylsilyl)oxy)butan-2-one (22.0 g), 2-aminonicotinaldehyde (9.62 g), proline (4.6 g), and ethanol (120 mL) was refluxed for 10 hours. 4-((tert-Butyldimethylsilyl)oxy)butan-2-one (10 g) was added thereto, and the mixture was refluxed for 10 hours. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (hexane/ethyl acetate=2/1 to 3/1) to obtain compound (I9) (2.57 g). LC/MS (SunFire) rt (min): 13.94 MS (ESI, m/z): 289.40 [M+H]+
(10) Compound (19) (2.50 g), methanol (75 mL), ethanol (75 mL), and 10% Pd/C (450 mg) were placed in an autoclave and stirred for 4 hours in a 4 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (I10) (2.5 g). HPLC (SunFire) rt (min): 10.19
(11) A mixture of compound (I10) (2.5 g), THF (25 mL), DIEA (7.5 mL), and di-tert-butyl dicarbonate (6 mL) was stirred at 70° C. for 11 hours. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=10/1) to obtain compound (I11) (1.85 g). TLC Rf: 0.85 (hexane/ethyl acetate=1/2)
(12) To a solution of compound (I11) (1.85 g) in THF (25 mL), a 1 mol/L solution of tetrabutyl ammonium fluoride in THF (8 mL) was added, and the mixture was stirred at room temperature for 3 hours. Then, ethyl acetate (50 mL) and a saturated aqueous solution of ammonium chloride (50 mL) were added thereto. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (100 mL) twice. The organic layers were combined, then washed with a saturated aqueous solution of sodium chloride, and then dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=80/20 to 30/70) to obtain compound (I12) (1.17 g). LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 279.4 [M+H]+
(13) To a solution of compound (I12) (1.17 g), triphenylphosphine (1.32 g), and 3-methyl-4-nitrophenol (837 mg) in THF (15 mL), diisopropyl azodicarboxylate (1.5 mL) was added dropwise over 5 minutes, and the mixture was stirred at room temperature for 3.5 hours. Ethyl acetate (100 mL) and a saturated aqueous solution of sodium bicarbonate (100 mL) were added thereto. The organic layer was separated and washed with a saturated aqueous solution of sodium chloride. After drying over anhydrous sodium sulfate, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=85/15 to 60/40) to obtain a fraction containing compound (I13). The solvent was distilled off under reduced pressure. To the obtained residue, DMF (30 mL), benzyl bromide (3 mL), and cesium carbonate (7.6 g) were added, and the mixture was stirred at room temperature for 30 minutes. Ethyl acetate (300 mL) and water (100 mL) were added thereto. The organic layer was separated and dried over anhydrous sodium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=80/20 to 60/40) to obtain compound (I13) (1.53 g). LC/MS (ACQUITY) rt (min): 1.27 MS (ESI, m/z): 414.5 [M+H]+
(14) To a solution of compound (I13) (1.53 g) in DMF (15 mL), DMFDA (2.5 mL) and pyrrolidine (1.4 mL) were added, and the mixture was stirred at 80° C. for 5 hours. Water (50 mL) and ethyl acetate (150 mL) were added thereto. The organic layer was separated, then washed with water and a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=70/30 to 40/60) to obtain compound (I14) (0.93 g). MS (ESI, m/z):495.3 [M+H]+
(15) Compound (114) (930 mg), methanol (20 mL), and 10% Pd/C (200 mg) were placed in a sealed tube and stirred for 3 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=75/25 to 70/30) to obtain compound (I15) (571 mg). TLC Rf: 0.36 (hexane/ethyl acetate=1/1) LC/MS (SunFire) rt (min): 9.15 MS (ESI, m/z): 394.10 [M+H]+
(16) To compound (I15) (152.4 mg), compound (I8) (240 mg), and cesium fluoride (53 mg), DMF (2.5 mL) was added, and the mixture was stirred at 70° C. for 5 hours. Ethyl acetate (30 mL) was added thereto, and the mixture was washed with water and a saturated aqueous solution of sodium chloride. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=100/0 to 90/10) to obtain compound (I16) (159 mg). LC/MS (SunFire) rt (min): 10.85, 11.17 MS (ESI, m/z): 847.20 [M+H]+
(17) Compound (116) (159 mg), methanol (15 mL), and 10% Pd/C (80 mg) were placed in a sealed tube and stirred for 6 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The residue was purified by preparative HPLC to obtain compound (I17) (44.6 mg). LC/MS (SunFire) rt (min): 5.92 MS (ESI, m/z): 615.15 [M+H]+
(18) To a solution of compound (I17) (27.4 mg) and Fmoc-cysteic acid (39.3 mg) in DMF (0.8 mL) and DIEA (30 μL), a solution of HBTU (37.4 mg) in DMF (0.2 mL) was added, and the mixture was stirred at room temperature for 1 hour. Water (0.1 mL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (I18) (12.0 mg). LC/MS (SunFire) rt (min): 11.97 MS (ESI, m/z): 494.90 [M+2H]2+, 986.15 [M−H]−
(19) To a solution of compound (I18) (6.7 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 11 hours.
The solvent was distilled off under reduced pressure. To the obtained residue, Fmoc-cysteic acid (10.6 mg), DMF (0.4 mL), and DIEA (20 μL) were added, then a solution of HBTU (9.5 mg) in DMF (0.1 mL) was added, and the mixture was stirred at room temperature for 1 hour. Water (0.2 mL) was added thereto, and the mixture was stirred for 20 minutes. Then, pyrrolidine (0.3 mL) was added thereto, and the mixture was stirred for 30 minutes. The reaction mixture was concentrated under reduced pressure, and the residue was purified by preparative HPLC to obtain compound (I19) (4.5 mg). LC/MS (SunFire) rt (min): 8.90 MS (ESI, m/z): 917.20 [M+H]+, 459.30 [M+2H]2+, 915.10 [M−H]−
(20) To a solution of compound (I3) (11.4 mg) in DMF (200 μL) and DIEA (10 μL), a solution of HBTU (6.4 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (I19) (4.5 mg) in DMF (200 μL) and DIEA (10 μL), and the resulting mixture was stirred at room temperature for 45 minutes. Water (100 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (I20) (5.2 mg). LC/MS (SunFire) rt (min): 10.43 MS (ESI, m/z): 787.60 [M+2H]2+
(21) A mixture of compound (I20) (5.2 mg), THF (1 mL), water (140 μL), and a 3 mol/L aqueous lithium hydroxide solution (100 μL) was stirred at room temperature for 3 hours. The solvent was distilled off under reduced pressure. Then, a 50% aqueous acetonitrile solution (400 μL) and formic acid (14 μL) were added to the residue, and the mixture was purified by preparative HPLC to obtain compound (I21) (1.5 mg). LC/MS (SunFire) rt (min): 8.01 MS (ESI, m/z): 638.45 [M+2H]2+, 425.65 [M+3H]3+
(1) To sodium hydride (60% suspension in mineral oil, 1.65 g), DMSO (40 mL) was added, and the mixture was heated to 80° C. and then cooled to room temperature. Methyl triphenylphosphonium bromide (14.7 g) was added thereto, and the mixture was stirred for 10 minutes. Then, a solution of 5,6,7,8-tetrahydro-1,8-naphthyridine-2-carbaldehyde (2.46 g) in DMSO (25 mL) was added thereto, and the mixture was stirred at room temperature for 30 minutes. Water (600 mL) and ethyl acetate (300 mL) were added thereto. Then, the organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (300 mL). The organic layers were combined, then washed with a saturated aqueous solution of sodium chloride (300 mL), and dried over anhydrous sodium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=4/1) to obtain compound (J1) (1.13 g). TLC Rf: 0.19 (hexane/ethyl acetate=2/1)
(2) To compound (J1) (1.37 g), di-tert-butyl dicarbonate (3.9 mL), DIEA (3.3 mL), and THF (15 mL) were added, and the mixture was refluxed for 3 days. The solvent was distilled off under reduced pressure, and ethyl acetate (50 mL) and a saturated aqueous solution of sodium chloride (50 mL) were added to the residue. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (50 mL). The organic layers were combined, then washed with a saturated aqueous solution of sodium chloride (50 mL), and dried over anhydrous magnesium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=8/1 to 7/1) to obtain compound (J2) (1.71 g). TLC Rf: 0.51 (hexane/ethyl acetate=2/1)1H-NMR (400 MHz, CDCl3) δ: 7.34 (1H, d, J=10.2 Hz), 6.96 (1H, d, J=10.2 Hz), 6.73 (1H, dd, 14.2, 23.1 Hz), 6.22 (1H, dd, 2.2, 23.1 Hz), 5.39 (1H, dd, 2.2, 14.2 Hz), 3.77 (2H, t, J=8.6 Hz), 2.75 (2H, t, J=8.8 Hz), 1.93 (2H, tt, J=8.6, 8.8 Hz), 1.47 (9H, s)
(3) To a mixture of compound (J2) (1.71 g), 4-bromoindole (824 μL), DMF (25 mL), and triethylamine (4 mL), palladium(II) acetate (147 mg) and (2-biphenyl)di-tert-butylphosphine (392 mg) were added, and the resulting mixture was stirred at 110° C. for 20 hours. Ethyl acetate (300 mL) and water (100 mL) were added thereto. The organic layer was separated, then washed twice with a saturated aqueous solution of sodium chloride (100 mL), and dried over anhydrous magnesium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=9/1 to 5/1) to obtain compound (J3) (1.18 g). TLC Rf: 0.31 (hexane/ethyl acetate=2/1) MS (ESI, m/z): 376.2 [M+H]+
(4) To compound (J3) (150 mg), compound (I8) (180 mg), and cesium fluoride (60 mg), DMF (1.5 mL) was added, and the mixture was stirred at 60° C. for 20 hours. Ethyl acetate (10 mL) was added thereto, and the mixture was washed with water and a saturated aqueous solution of sodium chloride and dried over anhydrous sodium sulfate. Then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=10/0 to 9/1) to obtain compound (J4) (130 mg). TLC Rf: 0.2 (ethyl acetate) LC/MS (SunFire) rt (min): 12.15, 12.68 MS (ESI, m/z): 415.25 [M+2H]2+
(5) Compound (J4) (130 mg), methanol (20 mL), and 10% Pd/C (100 mg) were placed in a sealed tube and stirred for 9 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The residue was purified by preparative HPLC to obtain compound (J5) (29.0 mg). HPLC (SunFire) rt (min): 7.42 LC/MS (SunFire) rt (min): 6.66 MS (ESI, m/z): 599.35 [M+H]+
(6) To a solution of compound (J5) (29.0 mg) and Fmoc-cysteic acid (48.0 mg) in DMF (1 mL) and DIEA (60 μL), a solution of HBTU (45.9 mg) in DMF (0.4 mL) was added, and the mixture was stirred at room temperature for 1 hour. Water (1 mL) was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, DMF (1 mL) and diethylamine (1 mL) were added, and the mixture was left at room temperature for 13 hours. The solvent was distilled off under reduced pressure. To the obtained residue, a 50% aqueous acetonitrile solution was added, and the mixture was purified by preparative HPLC to obtain compound (J6). HPLC (SunFire) rt (min): 9.80
(7) To compound (J6) obtained in the step (6), a solution of Fmoc-cysteic acid (48.0 mg), DMF (0.6 mL), DIEA (60 μL), and HBTU (45.9 mg) in DMF (0.4 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. Water (0.5 mL) was added thereto, and the mixture was stirred for 5 minutes. Then, pyrrolidine (0.5 mL) was added thereto, and the mixture was stirred at room temperature for 30 minutes. The solvent was distilled off under reduced pressure. The obtained residue was purified by preparative HPLC to obtain compound (J7) (8.0 mg). HPLC (SunFire) rt (min): 9.17 LC/MS (SunFire) rt (min): 9.23 MS (ESI, m/z): 901.10 [M+H]+, 451.35 [M+2H]2+, 899.00 [M−H]−
(8) To a solution of compound (I3) (16.9 mg) in DMF (200 μL) and DIEA (20 μL), a solution of HBTU (9.5 mg) in DMF (60 μL) was added, then the mixture was added to a solution of compound (J7) (8.0 mg) in DMF (200 μL) and DIEA (10 μL), and the resulting mixture was stirred at room temperature for 2 hours. Water (100 μL) was added thereto, and then, the mixture was purified by preparative HPLC to obtain compound (J8) (6.9 mg). LC/MS (SunFire) rt (min): 10.46 MS (ESI, m/z): 779.65 [M+2H]2+
(9) A mixture of compound (J8) (4.2 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (70 μL) was stirred at room temperature for 4 hours. The solvent was distilled off under reduced pressure. To the obtained residue, a 50% aqueous acetonitrile solution (400 μL) and formic acid (10 μL) were added, and the mixture was purified by preparative HPLC to obtain compound (J9) (1.2 mg). LC/MS (SunFire) rt (min): 8.62 MS (ESI, m/z): 630.45 [M+2H]2+, 628.05 [M−2H]2−
(1) To a mixture of (R)-methyl 2-amino-3-((tert-butoxycarbonyl)amino) propanoate hydrochloride (3.92 g), acetonitrile (39 mL), and potassium carbonate (6.4 g), 4-(4-(chlorosulfonyl)-3,5-dimethylphenyloxy) butanoic acid (4.32 g) was added in 4 divided portions every 30 minutes, and then, the resulting mixture was stirred at room temperature for 9 hours. Water (150 mL) and ethyl acetate (50 mL) were added thereto. The aqueous layer was separated, and sodium chloride (20 g) and ethyl acetate (50 mL) were added thereto. The reaction mixture was neutralized with concentrated hydrochloric acid, and the separated organic layer was washed twice with a saturated aqueous solution of sodium chloride (100 mL) and then dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure to obtain compound (K1) (3.13 g). HPLC (CAPCELL PAK MG) rt (min): 14.28 LC/MS (ACQUITY) rt (min): 1.31 MS (ESI, m/z): 487.4 [M−H]−
(2) To a mixture of compound (K1) (3.13 g), DMF (13 mL), Z-ethylenediamine hydrochloride (1.48 g), and DIEA (2.3 mL), HBTU (2.55 g) was added, and the resulting mixture was stirred at room temperature for 1 hour. Water (16 mL) was added dropwise thereto, and the mixture was stirred for 2 hours. Then, water (16 mL) was added thereto, and the solid was collected by filtration to obtain compound (K2) (3.40 g). HPLC (CAPCELL PAK MG) rt (min): 14.98 LC/MS (ACQUITY) rt (min): 1.46 MS (ESI, m/z): 665.5 [M+H]+, 663.6 [M−H]−
(3) To a solution of compound (K2) (3.04 g) in dichloromethane (10 mL), TFA (10 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. The solvent was distilled off under reduced pressure, and a 4 mol/L solution of hydrogen chloride in dioxane (10 mL) was added to the residue. The solvent was distilled off under reduced pressure. To the obtained residue, compound (O4) (1.04 g), DMF (16 mL), DIEA (2.4 mL), and HBTU (1.91 g) were added, and the mixture was stirred at room temperature for 1.5 hours. A 5% aqueous sodium bicarbonate solution (80 mL) and ethyl acetate (80 mL) were added thereto, and the mixture was stirred at room temperature for 10 minutes. The organic layer was separated, then washed twice with a saturated aqueous solution of sodium chloride (50 mL), and dried over anhydrous sodium sulfate, and then, the solvent was distilled off under reduced pressure. Ethyl acetate (16 mL) was added to the residue, and the solid was collected by filtration to obtain compound (K3) (2.52 g). LC/MS (ACQUITY) rt (min): 1.16 MS (ESI, m/z): 781.7 [M+H]+
(4) A mixture of 10% Pd/C (0.40 g), methanol (25 mL), and compound (K3) (1.90 g) was stirred at room temperature for 17 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (K4) (1.72 g). LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 647.6 [M+H]+, 645.6 [M−H]−
(5)
To a mixture of compound (K4) (183 mg), Z-cysteic acid (85.8 mg), DMF (2 mL), and DIEA (172 μL), HBTU (113 mg) was added, and the resulting mixture was stirred at room temperature for 1 hour. Water (10 mL) and acetic acid (0.5 mL) were added thereto. The organic layer was separated and washed with water (10 mL), and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (chloroform/methanol=95/5 to 65/35) to obtain compound (K5) (110 mg). LC/MS (ACQUITY) rt (min): 1.05 MS (ESI, m/z): 932.8 [M+H]+, 930.9 [M−H]−
(6) A mixture of compound (K5) (110 mg), 10% Pd/C (50 mg), and methanol/water (9/1) (14 mL) was stirred at room temperature for 2.5 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (K6) (84.3 mg). LC/MS (ACQUITY) rt (min): 0.80 MS (ESI, m/z): 798.7 [M+H]+, 796.8 [M−H]−
(7) To a mixture of compound (K6) (84.3 mg), tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (60.7 mg), DMF (1 mL), and DIEA (50 μL), HBTU (40.2 mg) was added, and the resulting mixture was stirred at room temperature for 30 minutes. Water (1 mL), methanol (0.5 mL), and formic acid (200 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (K7) (61.9 mg). LC/MS (ACQUITY) rt (min): 1.12 MS (ESI, m/z): 677.4 [M+2H]2+, 1351.3 [M−H]−
(8) To compound (K7) (29 mg), concentrated hydrochloric acid (2 mL) was added, and the mixture was stirred at room temperature for 3 days. The solvent was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (2 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (K8) (11.0 mg). LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 586.1 [M+2H]2+, 584.0 [M−2H]2−
(1) To a mixture of 2-amino-6-bromopyridine (1.73 g), THF (20 mL), DMAP (120 mg), and DIEA (7 mL), di-tert-butyl dicarbonate (4.6 mL) was added, and the resulting mixture was stirred at room temperature for 3 hours. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=3/1) to obtain compound (L1) (3.09 g). 1H-NMR (400 MHz, CDCl3) δ: 7.55-7.59 (1H, m), 7.37-7.39 (1H, m), 7.27 (1H, m), 1.46 (18H, s)
(2) To a mixture of ethyl 4-ethynylbenzoate (0.97 g), compound (L1) (1.44 g), acetonitrile (20 mL), and triethylamine (10 mL), dichlorobis(triphenylphosphine)palladium(II) (78.2 mg) and copper(I) iodide (32 mg) were added, and the resulting mixture was heated at 70° C. for 200 minutes. After cooling to room temperature, ethyl acetate (30 mL) and water (30 mL) were added thereto. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (30 mL). The organic layers were combined, then washed with water and a saturated aqueous solution of sodium chloride, and dried over anhydrous magnesium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/acetone=20/1 to 10/1) to obtain compound (L2) (1.22 g). TLC Rf: 0.63 (hexane/ethyl acetate=2/1) LC/MS (SunFire) rt (min): 14.63 MS (ESI, m/z): 467.10 [M+H]+
(3) Compound (L2) (1.10 g), methanol (150 mL), and 10% Pd/C (300 mg) were placed in an autoclave and stirred for 8 hours in a 3 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (L3) (1.17 g). TLC Rf: 0.59 (hexane/ethyl acetate=2/1) LC/MS (SunFire) rt (min): 14.53 MS (ESI, m/z): 493.10 [M+Na]+
(4) To a solution of compound (L3) (610 mg) in methanol (15 mL), a solution of sodium hydroxide (0.29 g) in water (1 mL) was added, and the mixture was stirred at room temperature for 1 hour. Sodium hydroxide (0.40 g), water (5 mL), and THF (5 mL) were added thereto, and the mixture was stirred for 4 hours. About half the amount of the solvent was distilled off under reduced pressure. Water (20 mL) was added to the residue, and the mixture was adjusted to pH 4 by the addition of sodium bisulfate. Ethyl acetate (30 mL) and water (30 mL) were added thereto. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (50 mL). The organic layers were combined, then washed with a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (L4) (0.51 g). TLC Rf: 0.24 (hexane/ethyl acetate=2/1) MS (ESI, m/z): 343.1 [M+H]+, 341.2 [M−H]−
(5) To a mixture of compound (A2) (390 mg), compound (L4) (208 mg), DMF (5 mL), and DIEA (0.6 mL), a solution of HBTU (235 mg) in DMF (2 mL) was added, and the resulting mixture was stirred at room temperature for 1 hour. Ethyl acetate (50 mL) and water (30 mL) were added thereto. The organic layer was separated, then washed with a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=40/60 to 0/100) to obtain compound (L5) (599 mg). LC/MS (SunFire) rt (min): 13.67 MS (ESI, m/z): 889.40 [M+H]+, 887.35 [M−H]−
(6) Compound (L5) (599 mg), methanol (30 mL), and 10% Pd/C (100 mg) were placed in a sealed tube and stirred for 6 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (L6) (476 mg). LC/MS (SunFire) rt (min): 9.64 MS (ESI, m/z): 755.35 [M+H]+, 753.40 [M−H]−
(7) To a solution of compound (L6) (129 mg) and Fmoc-cysteic acid (145 mg) in DMF (1 mL) and DIEA (70 μL), a solution of HBTU (138 mg) in DMF (1 mL) was added, and the mixture was stirred at room temperature for 30 minutes. DIEA (0.1 mL) was added thereto, and the mixture was stirred for 2 hours. Water (0.1 mL) was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, DMF (0.8 mL) and diethylamine (0.8 mL) were added, and the mixture was stirred at room temperature for 15 minutes. Then, the solvent was distilled off under reduced pressure. The obtained residue was purified by preparative HPLC to obtain compound (L7) (77.8 mg). LC/MS (SunFire) rt (min): 11.59 MS (ESI, m/z): 906.25 [M+H]+, 904.20 [M−H]−
(8) To a mixture of compound (L7) (51.6 mg) and (S)-bis(2,5-dioxopyrrolidin-1-yl) 2-((tert-butoxycarbonyl)amino)pentanedioate (12.5 mg), DMF (0.4 mL) and DIEA (30 μL) were added, and the resulting mixture was stirred at room temperature for 24 hours. Water (0.1 mL) was added thereto, and then, the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 1 hour. TFA was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (1.5 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (L8) (24.8 mg). LC/MS (SunFire) rt (min): 7.86 MS (ESI, m/z): 862.05 [M+2H]2+, 859.95 [M−2H]2−
(9) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (5.2 mg) in DMF (0.1 mL) and DIEA (10 μL), a solution (75 μL) of HBTU (4.4 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (L8) (9.6 mg) in DMF (200 μL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 50 minutes. Water (200 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (L9) (7.2 mg). LC/MS (SunFire) rt (min): 8.98 MS (ESI, m/z): 1139.70 [M+2H]2+, 760.15 [M+3H]3+, 1137.35 [M−2H]2−
(10) To compound (L9) (7.6 mg), THF (0.3 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (70 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and then, the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. TFA was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (0.2 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (L10) (2.3 mg). LC/MS (SunFire) rt (min): 8.61 MS (ESI, m/z): 1041.55 [M+2H]2+, 694.55 [M+3H]3+, 1039.35 [M−2H]2−
(1) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (24.8 mg) in DMF (0.2 mL) and DIEA (17 μL), a solution of HBTU (17.0 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (L7) (13.1 mg) in DMF (200 μL) and DIEA (10 μL), and the resulting mixture was stirred at room temperature for 1 hour. Water (200 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (M1) (12.3 mg). LC/MS (SunFire) rt (min): 11.05 MS (ESI, m/z): 730.95 [M+2H]2+, 1459.05 [M−H]−
(2) To compound (M1) (7.8 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (70 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 2 hours. TFA was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (0.8 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (M2) (1.9 mg). LC/MS (SunFire) rt (min): 7.34 MS (ESI, m/z): 590.10 [M+2H]2+, 588.25 [M−2H]2−
(1) To a solution of Fmoc-cysteic acid (22.0 mg) in DMF (0.2 mL) and DIEA (20 μL), a solution of HBTU (21.0 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (L7) (16.3 mg) in DMF (0.4 mL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 1 hour. Water (0.1 mL) was added thereto, and the solvent was distilled off under reduced pressure. DMF (0.5 mL) and diethylamine (0.5 mL) were added to the residue, and the mixture was left at room temperature for 15 hours. The solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 20 minutes. TFA was distilled off. The obtained residue was purified by preparative HPLC to obtain compound (N1) (9.6 mg). LC/MS (SunFire) rt (min): 8.93 MS (ESI, m/z): 957.10 [M+H]+, 955.15 [M−H]−
(2) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (8.3 mg) in DMF (0.1 mL) and DIEA (10 μL), a solution of HBTU (5.5 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (N1) (4.6 mg) in DMF (200 μL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 1.5 hours. Water (100 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (N2) (3.5 mg). LC/MS (SunFire) rt (min): 9.69 MS (ESI, m/z): 756.70 [M+2H]+, 1509.45 [M−H]−
(3) To compound (N2) (3.5 mg), THF (0.7 mL), water (0.1 mL), and a 3 mol/L aqueous lithium hydroxide solution (70 μL) were added, and the mixture was stirred at room temperature for 2 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 1 hour. TFA was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (0.4 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (N3) (0.9 mg). LC/MS (SunFire) rt (min): 10.28 MS (ESI, m/z): 665.15 [M+2H]2+
(1) To a solution of 6-oxoheptanoic acid (99.2 g) in methanol (1 L), concentrated sulfuric acid (20 mL) was added, and the mixture was heated to reflux for 4 hours. The reaction mixture was cooled to room temperature, and then, the solvent was distilled off under reduced pressure. Water (1 L) and ethyl acetate (600 mL) were added to the residue. The organic layer was separated and washed with a 5% aqueous sodium bicarbonate solution (600 mL) and a saturated aqueous solution of sodium chloride (600 mL), and the solvent was distilled off under reduced pressure to obtain compound (O1) (95.2 g). TLC Rf: 0.45 (hexane/ethyl acetate=2/1)
(2) To a mixture of 2-aminonicotinaldehyde (133 g) and methanol (500 mL), compound (O1) (189 g) and methanol (600 mL) were added, then pyrrolidine (100 mL) was added, and the resulting mixture was heated to reflux for 8 hours. The reaction mixture was cooled to room temperature, and the solvent was distilled off under reduced pressure. Then, toluene (100 mL) was added to the residue, and the solvent was distilled off under reduced pressure. To the obtained residue, toluene (150 mL) was added, and the mixture was stirred at 50° C. for 2 hours and then stirred at room temperature for 3 hours. The solid was collected by filtration to obtain compound (O2) (149 g). TLC Rf: 0.56 (ethyl acetate/methanol=5/1) LC/MS (ACQUITY) rt (min): 0.73 MS (ESI, m/z): 245.2 [M+H]+
(3) 10% Pd/C (10.0 g), compound (O2) (97.5 g), and methanol (250 mL) were placed in an autoclave and stirred for 8 hours in a 5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. To the obtained residue, acetonitrile (100 mL) was added, and the solid was collected by filtration to obtain compound (O3) (71.5 g). P HPLC (CAPCEL PAK MG) rt (min): 8.06 1H-NMR (300 MHz, CDCl3) δ: 7.05 (1H, d, J=7.5 Hz), 6.34 (1H, d, 7.5 Hz), 4.74 (1H, brs), 3.66 (3H, s), 3.37-3.42 (2H, m), 2.68 (2H, t, J=6.0 Hz), 2.52-2.57 (2H, m), 2.30-2.37 (2H, m), 1.90 (2H, tt, J=5.7, 6.0 Hz), 1.63-1.70 (4H, m)
(4) To compound (O3) (70.0 g), methanol (210 mL) was added. After dissolution by heating at 40° C., a mixture of sodium hydroxide (16.9 g) and water (105 mL) was added dropwise to the solution over 15 minutes, and the resulting mixture was stirred at 40° C. for 1 hour. The solvent was distilled off under reduced pressure. Water (210 mL) was added to the residue, and the mixture was heated to 40° C. Concentrated hydrochloric acid was added dropwise thereto such that the temperature was kept at 50° C. or lower until the pH reached 5. Water (50 mL) was added thereto, and the mixture was cooled to room temperature and left all night and all day. The solid matter was collected by filtration to obtain compound (O4) (62.2 g). HPLC (CAPCEL PAK MG) rt (min): 7.03 LC/MS (ACQUITY) rt (min): 0.62 MS (ESI, m/z): 235.2 [M+H]+
(5) To a mixture of compound (A2) (7.40 g), compound (O4) (3.37 g), DMF (50 mL), and DIEA (3.86 mL), HBTU (4.98 g) was added in small portions, and the resulting mixture was stirred at room temperature for 2 hours. A 5% aqueous sodium bicarbonate solution (200 mL) and ethyl acetate (200 mL) were added to the reaction mixture, and the mixture was stirred at room temperature for 10 minutes. The organic layer was separated, then washed three times with a saturated aqueous solution of sodium chloride, and dried over anhydrous sodium sulfate, and then, the solvent was distilled off under reduced pressure. To the obtained residue, ethyl acetate (50 mL) was added, and the solid matter was collected by filtration to obtain compound (O5) (9.20 g). LC/MS (ACQUITY) rt (min): 1.12 MS (ESI, m/z): 781.5 [M+H]+, 779.6 [M−H]−
(6) To compound (O5) (7.20 g) and 10% Pd/C (300 mg), methanol (40 mL) was added, and the mixture was stirred at room temperature for 3 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. To the obtained residue, toluene (50 mL) was added, and the solvent was distilled off under reduced pressure to obtain compound (O6) (5.45 g). LC/MS (ACQUITY) rt (min): 0.73 MS (ESI, m/z): 647.4 [M+H]+
(7) To a solution of compound (O6) (120 mg) and Fmoc-cysteic acid (145 mg) in DMF (2 mL) and DIEA (140 μL), a solution of HBTU (141 mg) in DMF (1.5 mL) was added, and the mixture was stirred at room temperature for 20 minutes. Water (2 mL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (O7) (87.7 mg). LC/MS (SunFire) rt (min): 11.83 MS (ESI, m/z): 1020.25 [M+H]+, 1018.50 [M−H]−
(8) To a solution of compound (O7) (29.8 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 80 minutes. The solvent was distilled off under reduced pressure. To the obtained residue, Fmoc-cysteic acid (22.8 mg), DMF (0.7 mL), and DIEA (22 μL) were added, then a solution of HBTU (22.2 mg) in DMF (200 μL) was added, and the mixture was stirred at room temperature for 30 minutes. Water (0.5 mL) was added thereto, and the solvent was distilled off under reduced pressure. Then, DMF (0.5 mL) and diethylamine (0.5 mL) were added to the residue, and the mixture was stirred at room temperature for 70 minutes. The solvent was distilled off under reduced pressure. Then, water (2 mL) was added to the residue, and the mixture was washed three times with hexane/ethyl acetate (1/1) (2 mL) and purified by preparative HPLC to obtain compound (O8) (13.2 mg). LC/MS (SunFire) rt (min): 8.47 MS (ESI, m/z): 949.15 [M+H]+, 947.20 [M−H]−
(9) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (23.9 mg) in DMF (200 μL) and DIEA (20 μL), a solution of HBTU (15.8 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (O8) (13.2 mg) in DMF (300 μL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 1 hour. Water (200 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (O9) (9.5 mg). LC/MS (SunFire) rt (rain): 9.19 MS (ESI, m/z): 752.70 [M+2H]2+, 1501.45 [M−H]−
(10) A mixture of compound (O9) (6.2 mg), THF (700 μL), water (100 μL), and a 3 mol/L aqueous lithium hydroxide solution (100 μL) was stirred at room temperature for 2 hours. TFA was added to the reaction mixture, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (0.5 mL) was added to the residue, and the mixture was stirred for 1.5 hours. Then, the solvent was distilled off under reduced pressure. To the obtained residue, a 20% aqueous acetonitrile solution (600 μL) and methanol (300 μL) were added, and the mixture was purified by preparative HPLC to obtain compound (O10) (1.6 mg). LC/MS (SunFire) rt (min): 11.49 MS (ESI, m/z): 661.35 [M+2H]2+, 1319.35 [M−H]−, 659.45 [M−2H]2−
(1) To a solution of compound (O7) (28.1 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. The solvent was distilled off under reduced pressure. To the residue, DMF (400 μL) and DIEA (20 μL) were added, then a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (31.6 mg), DMF (150 μL), DIEA (20 μL), and HBTU (20.9 mg) in DMF (150 mL) was added, and the mixture was stirred at room temperature for 45 minutes. Water (500 μL) was added thereto, followed by extraction with hexane/ethyl acetate (1/1) (0.5 mL) three times. Then, the extract was purified by preparative HPLC to obtain compound (P1) (19.6 mg). HPLC (SunFire) rt (min): 9.71 LC/MS (ACQUITY) rt (min): 1.12 MS (ESI, m/z): 1352.5 [M+H]+, 1350.6 [M−H]−
(2) To compound (P1) (11.8 mg), THF (1.4 mL), water (200 μL), and a 3 mol/L aqueous lithium hydroxide solution (200 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. To the obtained residue, TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred at room temperature for 100 minutes. The solvent was distilled off under reduced pressure. Water/acetonitrile (2/1) (1.8 mL) and formic acid (1.8 μL) were added to the residue, and the mixture was purified by preparative HPLC to obtain compound (P2) (8.9 mg). HPLC (SunFire) rt (min): 8.75 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1170.4 [M+H]+, 585.9 [M+2H]2+, 1168.4 [M−H]−
(1) To a mixture of 4-bromo-2-fluorophenol (4.71 g), NMP (25 mL), and potassium carbonate (5.1 g), ethyl 4-bromobutanoate (4.2 mL) was added at 90° C., and the resulting mixture was stirred at the same temperature as above for 5.5 hours. The reaction mixture was cooled to room temperature, and ethyl acetate and water were added thereto. The organic layer was separated, then washed with 4% hydrochloric acid, and then dried over anhydrous magnesium sulfate. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=9/1 to 7/3) to obtain compound (Q1) (7.3 g). TLC Rf: 0.48 (hexane/ethyl acetate=4/1)1H-NMR (300 MHz, CDCl3) δ: 7.15-7.25 (2H, m), 6.83 (1H, t, J=9.0 Hz), 4.15 (2H, q, J=7.2 Hz), 4.06 (2H, t, J=6.0 Hz), 2.52 (2H, t, J=7.5 Hz), 2.15 (2H, tt, J=7.5, 6.0 Hz), 1.27 (3H, t, J=7.2 Hz)
(2) To a mixture of compound (Q1) (7.0 g), tert-butyl acrylate (15 mL), NMP (20 mL), and triethylamine (20 mL), palladium(II) acetate (224 mg) and tri(o-tolyl)phosphine (609 mg) were added in a nitrogen atmosphere, and the resulting mixture was stirred at 110° C. for 8 hours. The reaction mixture was cooled to room temperature. Insoluble matter was filtered off, and the residue was washed with ethyl acetate (200 mL). The organic layers were combined, then washed twice with water (300 mL), then washed with a saturated aqueous solution of sodium chloride (300 mL), and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=9/1 to 4/1) to obtain compound (Q2) (5.38 g). TLC Rf: 0.40 (hexane/ethyl acetate=4/1) 1H-NMR (300 MHz, CDCl3) δ: 7.48 (1H, d, J=15.6 Hz), 7.17-7.26 (2H, m), 6.91 (1H, t, J=5.1 Hz), 6.22 (1H, d, J=15.6 Hz), 4.16 (2H, q, J=7.2 Hz), 4.11 (2H, t, J=6.0 Hz), 2.54 (2H, t, J=7.2 Hz), 2.15 (2H, tt, J=6.0, 7.2 Hz), 1.55 (9H, s), 1.26 (3H, t, J=7.2 Hz) LC/MS (ACQUITY) rt (min): 1.94 MS (ESI, m/z): 297.1 [M−tBu]+
(3) A solution of (R)-(+)-N-benzyl-1-phenylethylamine (5.13 g) in THF (50 mL) was cooled to −70° C., and butyllithium (1.62 mol/L solution in hexane, 13 mL) was added dropwise over 15 minutes such that the temperature was kept at −65° C. or lower. The temperature of the mixture was raised to −30° C. over 50 minutes. Then, the reaction mixture was cooled to −70° C., and a solution of compound (Q2) (4.21 g) in THF (20 mL) was added dropwise thereto over 15 minutes. The mixture was stirred at the same temperature as above for 2 hours, and a saturated aqueous solution of ammonium chloride (100 mL) was added thereto. Ethyl acetate (300 mL) and water (200 mL) were added to the mixture. The organic layer was separated, and the aqueous layer was subjected to extraction with ethyl acetate (200 mL). The organic layer and the extract were combined, then washed once with a 10% aqueous acetic acid solution (300 mL) and twice with a saturated aqueous solution of sodium chloride (300 mL), and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was recrystallized from IPA/hexane to obtain compound (Q3) (3.03 g). The filtrate of recrystallization was purified by silica gel column chromatography (hexane/ethyl acetate=10/0 to 9/1) to obtain compound (Q3) (2.75 g). TLC Rf: 0.52 (hexane/ethyl acetate=4/1) LC/MS (ACQUITY) rt (min): 2.32 MS (ESI, m/z): 564.3 [M+H]+
(4) Compound (Q3) (434 mg), ethanol (5 mL), acetic acid (0.4 mL), water (40 μL), and 10% Pd/C (100 mg) were placed in a sealed tube and stirred for 5 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the reaction mixture was neutralized with a saturated aqueous solution of sodium bicarbonate (50 mL), followed by extraction with ethyl acetate (100 mL) twice. The extract was washed with a saturated aqueous solution of sodium chloride and dried over anhydrous sodium sulfate to obtain compound (Q4) (290 m). TLC Rf: 0.15 (hexane/ethyl acetate=5/1) LC/MS (ACQUITY) rt (min): 1.15 MS (ESI, m/z): 370.2 [M+H]+
(5) To a solution of compound (Q4) (220 mg) and compound (A1) (140 mg) in DMF (7 mL) and DIEA (420 μL), HBTU (228 mg) was added, and the mixture was stirred at room temperature for 40 minutes. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=10/0 to 9/1) to obtain compound (Q5) (310 mg). TLC Rf: 0.74 (ethyl acetate/methanol=5/1) LC/MS (ACQUITY) rt (min): 1.39 MS (ESI, m/z): 634.4 [M+H]+
(6) To a mixture of compound (Q5) (289 mg), THF (2.8 mL), methanol (2 mL), and water (0.4 mL), a 2 mol/L aqueous lithium hydroxide solution (460 μL) was added, and the resulting mixture was stirred at room temperature for 2.5 hours. To the reaction mixture, water (10 mL) was added, and then, citric acid (300 mg) was added, followed by extraction with chloroform (15 mL) four times. The extract was dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (Q6) (181 mg). LC/MS (ACQUITY) rt (min): 1.20 MS (ESI, m/z): 606.3 [M+H]+, 604.3 [M−H]−
(7) To a mixture of compound (Q6) (181 mg), z-ethylenediamine hydrochloride (89.6 mg), DMF (2 mL), and DIEA (200 μL), HBTU (147 mg) was added, and the resulting mixture was stirred at room temperature for 20 minutes. Ethyl acetate (60 mL) and water (10 mL) were added to the reaction mixture. The organic layer was separated, then washed twice with water (30 mL), then washed with a saturated aqueous solution of sodium chloride (30 mL), and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (ethyl acetate/methanol=10/0 to 9/1) to obtain compound (Q7) (135 mg). LC/MS (ACQUITY) rt (min): 1.32 MS (ESI, m/z): 782.4 [M+H]+
(8) To a mixture of compound (Q7) (130 mg), ethanol (10 mL), and 10% Pd/C (50 mg), 1,4-cyclohexadiene (0.4 mL) was added at 60° C., and the resulting mixture was stirred at the same temperature as above for 2 hours. The reaction mixture was cooled to room temperature. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (Q8) (108 mg). LC/MS (ACQUITY) rt (min): 1.02 MS (ESI, m/z): 648.3 [M+H]+, 324.7 [M+2H]2+
(9) To a solution of compound (O8) (108 mg) and Fmoc-cysteic acid (78.2 mg) in DMF (3 mL) and DIEA (70 μL), HBTU (75.9 mg) was added, and the mixture was stirred at room temperature for 25 minutes. To the reaction mixture, water (0.1 mL) were added, then diethylamine (2 mL) was added, and the mixture was stirred at room temperature for 1.5 hours. Diethylamine was distilled off. Then, water (1 mL) was added to the residue, and the mixture was washed three times with ethyl acetate (2 mL) and then purified by preparative HPLC to obtain compound (Q9) (63.2 mg). HPLC (SunFire) solvent: solution A=10 mmol/L aqueous ammonium acetate solution, solution B=10 mmol/L ammonium acetate/methanol:acetonitrile (4:1)), gradient cycle: 0.0 min (solution A/solution B=80/20), 10 min (solution A/solution B=0/100), 15 min (solution A/solution B=0/100), flow rate: 1.0 mL/min) rt (min): 12.92 LC/MS (ACQUITY) rt (min): 1.08 MS (ESI, m/z): 799.3 [M+H]+, 797.3 [M−H]−
(10) To a solution of compound (Q9) (34.2 mg) and Fmoc-cysteic acid (33.5 mg) in DMF (0.6 mL) and DIEA (30 DL), a solution of HBTU (32.5 mg) in DMF (400 μL) was added, and the mixture was stirred at room temperature for 1 hour. To the reaction mixture, water (0.5 mL) was added, then diethylamine (1 mL) was added, and the mixture was stirred at room temperature for 1 hour. Diethylamine was distilled off. Then, water (1 mL) was added to the residue, and the mixture was washed three times with ethyl acetate (2 mL) and then purified by preparative HPLC to obtain compound (Q10) (13.9 mg). HPLC (SunFire) solvent: solution A=10 mmol/L aqueous ammonium acetate solution, solution B=10 mmol/L ammonium acetate/methanol:acetonitrile (4:1)), gradient cycle: 0.0 min (solution A/solution B=80/20), 10 min (solution A/solution B=0/100), 15 min (solution A/solution B=0/100), flow rate: 1.0 mL/min) rt (min): 12.16 LC/MS (ACQUITY) rt (min): 1.07 MS (ESI, m/z): 950.4 [M+H]+
(11) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (25.1 mg) in DMF (150 μL) and DIEA (30 μL), a solution of HBTU (16.7 mg) in DMF (150 μL) was added, then the mixture was added to a solution of compound (Q10) (13.9 mg) in DMF (400 μL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 45 minutes. Water (300 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (Q11) (11.1 mg). HPLC (SunFire) rt (min): 10.80 LC/MS (ACQUITY) rt (min): 1.24 MS (ESI, m/z): 1504.6 [M+H]+, 1502.6 [M−H]−
(12) To compound (Q11) (5.2 mg), TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. To the obtained residue, water (1 mL) and a 50% aqueous acetonitrile solution (0.2 mL) were added, and the mixture was purified by preparative HPLC to obtain compound (Q12) (3.7 mg). HPLC (SunFire) rt (min): 19.61 LC/MS (ACQUITY) rt (min): 0.80 MS (ESI, m/z): 1280.2 [M+H]+, 1278.4 [M−H]−
(1) Compound (R1) was obtained according to the method described in Bioconjugate Chemistry, 1991, Vol. 2, p. 187-194 and Bioconjugate Chemistry, 1991, Vol. 2, p. 180-186.
(2) A mixture of compound (A6) (12.4 mg), THF (1.4 mL), water (400 μL), and a 3 mol/L aqueous lithium hydroxide solution (200 μL) was stirred at room temperature for 2 hours. Formic acid (50 μL) was added to the reaction mixture, and the solvent was distilled off under reduced pressure. Water (1 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (R2) (6.9 mg). HPLC (SunFire) rt (min): 9.85 LC/MS (ACQUITY) rt (min): 0.83 MS (ESI, m/z): 983.4 [M+H], 981.3 [M−H]−, 490.1 [M−2H]2−
(3) To a mixture of compound (R2) (6.9 mg), compound (R1) (10.1 mg), DMF (800 μL), and DIEA (10 μL), water (300 μL) was added, and the resulting mixture was stirred at room temperature for 2 days. Water (400 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (R3) (7.5 mg). HPLC (SunFire) solvent: solution A=10 mmol/L aqueous ammonium acetate solution, solution B=10 mmol/L ammonium acetate/methanol:acetonitrile (4:1)), gradient cycle: 0.0 min (solution A/solution B=80/20), 10 min (solution A/solution B=0/100), 15 min (solution A/solution B=0/100), flow rate: 1.0 mL/min) rt (min): 7.26 LC/MS (ACQUITY) rt (min): 0.83 MS (ESI, m/z): 769.4 [M+2H]2+, 513.4 [M+3H]3+, 767.4 [M−2H]2−
(1) To a mixture of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (24.7 mg), DMF (150 μL), and DIEA (30 μL), a solution of HBTU (16.4 mg) in DMF (150 μL) was added, then the resulting mixture was added to a solution of compound (A4) (14.7 mg) in DMF (400 μL) and DIEA (20 μL), and the resulting mixture was stirred at room temperature for 50 minutes. Water (200 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (S1) (15.7 mg). HPLC (SunFire) rt (min): 8.87 LC/MS (ACQUITY) rt (min): 1.44 MS (ESI, m/z): 1249.6 [M+H]+, 1247.6 [M−H]−
(2) To compound (S1) (12.8 mg), THF (1 mL), water (200 μL), and a 3 mol/L aqueous lithium hydroxide solution (200 μL) were added, and the mixture was stirred at room temperature for 1.5 hours. TFA was added thereto, and then, the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (1 mL) was added to the residue, and the mixture was stirred at room temperature for 2 hours. TFA was distilled off. Water/acetonitrile (2/1) (1.8 mL) and formic acid (1.8 μL) were added to the residue, and the mixture was purified by preparative HPLC to obtain compound (S2) (9.2 mg). HPLC (SunFire) rt (min): 7.76 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 534.4 [M+2H]2+, 1065.4 [M−H]−
(1) To a solution of compound (O7) (120 mg) in DMF (1 mL), diethylamine (1 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. Water (1 mL) and a 3 mol/L aqueous lithium hydroxide solution (100 μL) were added to the residue, and the mixture was stirred at room temperature for 14 hours. A 50% aqueous acetonitrile solution (1 mL) and water (1 mL) were added thereto, and the mixture was washed with ethyl acetate (3 mL) and then purified by preparative HPLC to obtain compound (T1) (69.8 mg). HPLC (SunFire) rt (min): 8.10 LC/MS (ACQUITY) rt (min): 0.76 MS (ESI, m/z): 784.4 [M+H]+, 782.4 [M−H]−
(2) To a mixture of compound (T1) (19.2 mg), DMF (200 μL), and DIEA (10 μL), 2,2′,2″,2′″-(2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetrayl)tetraacetic acid (13.5 mg) was added, and the resulting mixture was stirred. DMF (600 μL), water (300 μL), and DIEA (30 μL) were added thereto, and the mixture was stirred at room temperature for 2 days. Water (1.1 mL) and formic acid (100 μL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (T2). HPLC (CAPCEL PAK MG) rt (min): 9.90 LC/MS (ACQUITY) rt (min): 0.80 MS (ESI, m/z): 1335.7 [M+H]+, 668.5 [M+2H]2+, 1333.7 [M−H]−
(1) To a mixture of compound (T1) (17.5 mg), DMF (200 μL), and DIEA (10 μL), compound (R1) (22.4 mg) was added, and the resulting mixture was stirred. DMF (600 DL), water (300 μL), and DIEA (30 μL) were added thereto, and the mixture was stirred at room temperature for 2 days. The obtained product was purified by preparative HPLC to obtain compound (U1). HPLC (SunFire) solvent: solution A=10 mmol/L aqueous ammonium acetate solution, solution B=10 mmol/L ammonium acetate/methanol:acetonitrile (4:1)), solution A/solution B=70/30, flow rate: 1.0 mL/min rt (min): 6.65 LC/MS (ACQUITY) rt (min): 0.93 MS (ESI, m/z): 669.7 [M+2H]+
(1) A mixture of ethyl 4-(1,8-naphthyridin-2-yl)butanoate (1.24 g), methanol (30 mL), and 10% Pd/C (205 mg) was stirred at room temperature for 16 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the residue was purified by silica gel column chromatography (ethyl acetate) to obtain compound (V1) (961 mg). TLC Rf: 0.43 (dichloromethane/methanol=95/5) MS (ESI, m/z): 249.3 [M+H]+
(2) To a mixture of compound (V1) (961 mg), THF (10 mL), and DIEA (1.8 mL), di-tert-butyl dicarbonate (1.8 mL) was added, and the resulting mixture was heated to reflux for 19 hours. The reaction mixture was cooled to room temperature, and ethyl acetate and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (dichloromethane to hexane/ethyl acetate=4/1) to obtain compound (V2) (1.09 g). TLC Rf: 0.50 (dichloromethane/methanol=100/1) MS (ESI, m/z): 349.3 [M+H]+
(3) To a solution of compound (V2) (1.1 g) in THF (8 mL) and methanol (8 mL), a 1 mol/L aqueous lithium hydroxide solution (5.3 mL) was added, and the mixture was stirred at room temperature for 14 hours. The reaction mixture was neutralized with hydrochloric acid, followed by extraction with ethyl acetate. The extract was dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (V3) (982 mg). TLC Rf: 0.52 (dichloromethane/methanol=9/1) MS (ESI, m/z): 321.3 [M+H]+
(4) To a solution of compound (V3) (130 mg) and compound (A2) (245 mg) in dichloromethane (7 mL) and DIEA (91 μL), HOBt (126 mg) and EDC.HCl (203 rag) were added, and the mixture was stirred at room temperature for 2 hours. Dichloromethane and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (dichloromethane/methanol=95/5) to obtain compound (V4) (255 mg). TLC Rf: 0.22 (dichloromethane/methanol=95/5) MS (ESI, m/z): 867.5 [M+H]+
(5) A mixture of compound (V4) (255 mg), methanol (8 mL), and 10% Pd/C (121 mg) was stirred at room temperature for 14 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (V5) (163 mg). TLC Rf: 0.05 (dichloromethane/methanol=10/1) MS (ESI, m/z): 733.5 [M+H]+
(6) To a mixture of disodium Fmoc-cysteinate (100 mg) and DMF (3 mL), methanesulfonic acid (22 μL) was added, and the resulting mixture was stirred for 5 minutes. DMF (5 mL), DIEA (200 μL), and compound (V5) (163 mg) were added thereto, and the mixture was stirred for 5 minutes. Then, HBTU (187 mg) was added thereto, and the mixture was stirred at room temperature for 2 hours. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (V6) (172 mg). TLC Rf: 0.25 (chloroform/ethanol/ammonia water=7/3/0.5) MS (ESI, m/z): 1104.2 [M−H]−
(7) To compound (V6) (108 mg), TFA (1 mL) was added, and the mixture was stirred at room temperature for 45 minutes. Then, the solvent was distilled off under reduced pressure. To the obtained residue, acetonitrile/methanol (9/1) (3 mL) and diethylamine (0.5 mL) were added, and the mixture was stirred for 1 hour. The solvent was distilled off under reduced pressure, and a 50% aqueous acetonitrile solution (3 mL), toluene (3 mL), and hexane (3 mL) were added to the residue. The aqueous layer was separated, and the solvent was distilled off under reduced pressure. To the obtained residue, DMF (1 mL) and DIEA (60 μL) were added, then a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (89.2 mg), DMF (300 μL), DIEA (20 μL), and HBTU (59.1 mg) in DMF (300 μL) was added, and the mixture was stirred at room temperature for 80 minutes. Water (2 mL) was added thereto, and then, the mixture was purified by preparative HPLC to obtain compound (V7) (25.9 mg). HPLC (SunFire) rt (min): 8.76 LC/MS (ACQUITY) rt (min): 1.28 MS (ESI, m/z): 670.2 [M+2H]2+, 1337.0 [M−H]−
(8) To compound (V7) (20.9 mg), TFA (1 mL) was added, and the mixture was stirred at room temperature for 5 hours. Then, the solvent was distilled off under reduced pressure. To the obtained residue, acetonitrile (1 mL) and TBME (1 mL) were added, and the solvent was distilled off under reduced pressure. To the obtained residue, water (0.6 mL) and a 3 mol/L aqueous lithium hydroxide solution (100 μL) were added, and the mixture was stirred at room temperature for 1 hour. Then, formic acid (20 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (V8) (9.6 mg). HPLC (SunFire) rt (min): 8.36 LC/MS (ACQUITY) rt (min): 0.71 MS (ESI, m/z): 1156.7 [M+H]+
(1) A mixture of 7-oxooctanoic acid (158 mg), ethanol (35 mL), and concentrated sulfuric acid (500 μL) was refluxed for 17 hours. The solvent was distilled off under reduced pressure, and then, a saturated aqueous solution of sodium bicarbonate and dichloromethane were added to the residue. The organic layer was separated and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (W1) (180 mg). MS (ESI, m/z): 187.2 [M+H]+
(2) A mixture of 2-aminonicotinaldehyde (118 mg), compound (W1) (180 mg), proline (56 mg), and methanol (10 mL) was refluxed for 18 hours. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (ethyl acetate) to obtain compound (W2) (90 mg). MS (ESI, m/z): 273.2 [M+H]+
(3) A mixture of compound (W2) (946 mg), methanol (20 mL), and 10% Pd/C (140 mg) was stirred at room temperature for 16 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (W3) (904 mg). MS (ESI, m/z): 277.3 [M+H]+
(4) To a solution of compound (W3) (900 mg) in THF (10 mL) and DIEA (1.5 mL), di-tert-butyl dicarbonate (1.5 mL) was added, and the mixture was refluxed for 25 hours. Ethyl acetate and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (dichloromethane/methanol=100/3) to obtain compound (W4) (904 mg). TLC Rf: 0.33 (dichloromethane/methanol=100/1) MS (ESI, m/z): 377.2 [M+H]+
(5) To a solution of compound (W4) (1.05 g) in THF (18 mL) and methanol (9 mL), a 1 mol/L aqueous lithium hydroxide solution (5 mL) was added, and the mixture was stirred at room temperature for 3 hours. The reaction mixture was neutralized with hydrochloric acid, followed by extraction with ethyl acetate. The extract was dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (W5) (970 mg). TLC Rf: 0.47 (dichloromethane/methanol=9/1) MS (ESI, m/z): 349.3 [M+H]+
(6) To a solution of compound (W5) (209 mg) and compound (A2) (265 mg) in dichloromethane (4 mL) and DIEA (120 μL), HOBt (122 mg) and EDC.HCl (233 mg) were added, and the mixture was stirred at room temperature for 3 hours. Dichloromethane and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (dichloromethane/methanol=95/5) to obtain compound (W6) (300 mg). TLC Rf: 0.12 (dichloromethane/methanol=95/5) MS (ESI, m/z): 895.7 [M+H]+
(7) A mixture of compound (W6) (300 mg), methanol (8 mL), and 10% Pd/C (126 mg) was stirred at room temperature for 16 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (W7) (194 mg). TLC Rf: 0.05 (dichloromethane/methanol=10/1) MS (ESI, m/z): 761.5 [M+H]+
(8) To a mixture of disodium Fmoc-cysteinate (120 mg) and DMF (3 mL), methanesulfonic acid (40 μL) was added, and the resulting mixture was stirred for 5 minutes. DMF (7 mL), DIEA (250 μL), and compound (W7) (194 mg) were added thereto, and the mixture was stirred for 5 minutes. Then, HBTU (201 mg) was added thereto, and the mixture was stirred at room temperature for 15 hours. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (W8) (216 mg). TLC Rf: 0.31 (chloroform/ethanol/ammonia water=7/3/0.5) MS (ESI, m/z): 1132.2 [M−H]−
(9) To compound (W8) (103 mg), TFA (1 mL) was added, and the mixture was stirred at room temperature for 45 minutes. Then, the solvent was distilled off under reduced pressure. To the obtained residue, acetonitrile/methanol (9/1) (3 mL) and diethylamine (0.5 mL) were added, and the mixture was stirred for 1 hour. The solvent was distilled off under reduced pressure, and a 50% aqueous acetonitrile solution (3 mL), toluene (3 mL), and hexane (3 mL) were added to the residue. The aqueous layer was separated, and the solvent was distilled off under reduced pressure. To the obtained residue, DMF (1 mL) and DIEA (60 μL) were added, then a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (90.1 mg), DMF (300 μL)., DIEA (60 μL), and HBTU (59.5 mg) in DMF (300 μL) was added, and the mixture was stirred at room temperature for 25 minutes. Water (2 mL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (W9) (57.7 mg). HPLC (SunFire) rt (min): 8.93 LC/MS (ACQUITY) rt (min): 1.26 MS (ESI, m/z): 1367.0 [M+H]+, 1365.0 [M−H]−
(10) To compound (W9) (21.6 mg), TFA (1 mL) was added, and the mixture was stirred at room temperature for 4 hours. Then, the solvent was distilled off under reduced pressure. To the obtained residue, acetonitrile (1 mL) and TBME (1 mL) were added, and the solvent was distilled off under reduced pressure. To the obtained residue, water (0.6 mL) and a 3 mol/L aqueous lithium hydroxide solution (100 μL) were added, and the mixture was stirred at room temperature for 1 hour. Then, formic acid (20 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (W10) (11.0 mg). HPLC (SunFire) rt (min): 8.94 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 1184.8 [M+H]+
(1) To a mixture of compound (J2) (1.5 g), ethyl 5-bromothiophene-2-carboxylate (1.42 g), triethylamine (3.5 mL), and DMF (22 mL), palladium(II) acetate (133 mg) and (2-(di-tert-butylphosphino)biphenyl (352 mg) were added, and the resulting mixture was stirred at 110° C. for 17 hours. The reaction mixture was cooled to room temperature, and ethyl acetate and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (hexane/ethyl acetate=9/1 to 5/1) to obtain compound (X1) (564 mg). TLC Rf: 0.55 (hexane/ethyl acetate=2/1) MS (ESI, m/z): 415.3 [M+H]+
(2) Compound (X1) (564 mg), methanol (20 mL), and 10% Pd/C (198 mg) were placed in a sealed tube and stirred at room temperature for 17 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the residue was purified by silica gel column chromatography (hexane/ethyl acetate=8/2) to obtain compound (X2) (498 mg). TLC Rf: 0.47 (hexane/ethyl acetate=2/1) MS (ESI, m/z): 417.3 [M+H]+
(3) To a solution of compound (X2) (498 mg) in THF (8 mL) and methanol (4 mL), a 1 mol/L aqueous lithium hydroxide solution (2 mL) was added, and the mixture was stirred at room temperature for 24 hours. To the reaction mixture, water (10 mL) were added, and then acetic acid was added until the solution became whitish. After extraction with ethyl acetate, the extract was dried over anhydrous sodium sulfate and then purified by silica gel column chromatography (dichloromethane/ethanol=9/1) to obtain compound (X3) (394 mg). TLC Rf: 0.49 (dichloromethane/methanol=9/1)1H-NMR (400 MHz, CDCl3) δ: 7.64 (1H, d, J=4.0 Hz), 7.31 (1H, d, J=8.8 Hz), 6.79 (1H, d, 4.0 Hz), 6.78 (1H, d, J=8.0 Hz), 3.78 (2H, t, J=6.4 Hz), 3.29 (2H, t, J=7.2 Hz), 3.10 (2H, t, J=7.2 Hz), 2.73 (2H, t, J=6.8 Hz), 1.93 (2H, tt, J=6.8, 6.4 Hz), 1.52 (9H, s) MS (ESI, m/z): 389.3 [M+H]+
(4) To a mixture of compound (A2) (415 mg), compound (X3) (238 mg), DMF (5 mL), and DIEA (540 μL), HBTU (290 mg) was added, and the resulting mixture was stirred at room temperature for 1 hour. Ethyl acetate and water were added to the reaction mixture. The organic layer was separated, then dried over anhydrous sodium sulfate, and purified by silica gel column chromatography (dichloromethane/methanol=95/5) to obtain compound (X4) (570 mg). TLC Rf: 0.41 (ethyl acetate) MS (ESI, m/z): 935.5 [M+H]+
(5) Compound (X4) (187 mg), methanol (15 mL), and 10% Pd/C (61 mg) were placed in a sealed tube and stirred for 18 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (dichloromethane/methanol=95/5 to chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (X5) (87 mg). TLC Rf: 0.27 (chloroform/ethanol/ammonia water=8/2/0.3) MS (ESI, m/z): 801.4 [M+H]+
(6) A mixture of disodium Fmoc-cysteinate (48 mg), DMF (1 mL), and methanesulfonic acid (8.5 μL) was stirred at room temperature for 30 minutes. DIEA (91 μL), DMF (2 mL), compound (X5) (87 mg), and HBTU (85 mg) were added thereto, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (X6) (106 mg). TLC Rf: 0.26 (chloroform/ethanol/ammonia water=7/3/0.5) MS (ESI, m/z): 1172.1 [M−H]−
(7) To a solution of compound (X6) (80 mg) in DMF (0.5 mL), diethylamine (0.5 mL) was added, and the mixture was stirred at room temperature for 80 minutes. The solvent was distilled off under reduced pressure. To the obtained oil, DMF (1 mL), DIEA (60 μL), Fmoc-cysteic acid (65.4 mg), and HBTU (63.3 mg) were added, and the mixture was stirred at room temperature for 20 minutes. To the reaction mixture, water (1 mL) was added, then diethylamine (2 mL) was added, and the mixture was stirred at room temperature for 30 minutes. Water (2 mL) was added thereto, and the mixture was washed twice with ethyl acetate (2 mL) and then purified by preparative HPLC to obtain compound (X7) (66.8 mg). HPLC (SunFire) rt (min): 9.36 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 989.5 [M+H]+, 987.4 [M−H]−
(8) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (39.0 mg) in DMF (150 μL) and DIEA (25 μL), a solution of HBTU (24.8 mg) in DMF (100 μL) was added, then the mixture was added to a solution of compound (X7) (27.3 mg) in DMF (0.3 mL) and DIEA (10 μL), and the resulting mixture was stirred at room temperature for 30 minutes. Water (0.5 mL) and acetonitrile (0.2 mL) were added thereto, and the mixture was purified by preparative HPLC to obtain compound (X8) (19.0 mg). HPLC (SunFire) rt (min): 9.89 LC/MS (ACQUITY) rt (min): 1.03 MS (ESI, m/z): 1543.7 [M+H]+, 1541.7 [M−H]−
(9) To compound (X8) (9.6 mg), TFA/triethylsilane (95/5) (1 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure. A 50% aqueous acetonitrile solution (1.2 mL) was added to the residue, and the mixture was purified by preparative HPLC to obtain compound (X9) (3.6 mg). HPLC (SunFire) rt (min): 10.08 LC/MS (ACQUITY) rt (min): 0.78 MS (ESI, m/z): 688.4 [M+2H]2+, 1373.5 [M−H]−, 686.5 [M−2H]2−
(1) To a mixture of phenol (2.84 g), DMF (40 mL), and potassium carbonate (7.9 g), ethyl 4-bromobutanoate (4.8 mL) was added, and the resulting mixture was stirred at room temperature for 22 hours. Ethyl acetate and water were added thereto. The organic layer was separated, and the solvent was distilled off under reduced pressure. To the obtained residue, ethanol (80 mL), water (20 mL), and sodium hydroxide (4.7 g) were added, and the mixture was stirred at room temperature for 4 hours. The solvent was distilled off under reduced pressure. The obtained residue was washed with hexane and then dissolved in water, and the solution was adjusted acidic with concentrated hydrochloric acid, followed by extraction with ethyl acetate. The organic layer was dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure to obtain compound (Y1) (5.08 g). TLC Rf: 0.38 (ethyl acetate)
(2) To a solution of compound (Y1) (5.08 g) in chloroform (30 mL), chlorosulfonic acid (10 mL) was added dropwise at 0° C. or lower over 30 minutes, and the mixture was stirred at 0° C. for 15 minutes. The reaction mixture was added to ice water, and the solid matter was collected by filtration to obtain compound (Y2) (4.25 g). TLC Rf: 0.38 (dichloromethane/methanol=95/5)
(3) To a mixture of (S)-2-amino-3-((tert-butoxycarbonyl)amino)propanoic acid methyl hydrochloride (1.0 g), dichloromethane (10 mL), and DIEA (1.4 mL), a solution of compound (Y2) (1.25 g) in dichloromethane (35 mL) was added, and the resulting mixture was stirred at room temperature for 70 hours. Water was added thereto. The organic layer was separated and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (dichloromethane/methanol=95/5) to obtain compound (Y3) (938 mg). TLC Rf: 0.16 (dichloromethane/methanol=95/5)
(4) To a mixture of compound (Y3) (938 mg), Z-ethylenediamine hydrochloride (517 mg), DMF (14 mL), and DIEA (1.4 mL), HATU (877 mg) was added, and the resulting mixture was stirred at room temperature for 1 hour. Dichloromethane and water were added to the reaction mixture. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (ethyl acetate) to obtain compound (Y4) (1.11 g). TLC Rf: 0.25 (dichloromethane/methanol=95/5)
(5) To a solution of compound (Y4) (1.11 g) in dichloromethane (10 mL), TFA (10 mL) was added, and the mixture was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and a saturated aqueous solution of sodium bicarbonate and dichloromethane were added to the residue. The organic layer was separated and dried over anhydrous sodium sulfate to obtain compound (Y5) (894 mg). TLC Rf: 0.20 (dichloromethane/methanol=9/1)
(6) To a solution of compound (Y5) (894 mg) and compound (H4) (640 mg) in DMF (15 mL) and DIEA (1.46 mL), HATU (673 mg) was added, and the mixture was stirred at room temperature for 2 hours. Ethyl acetate and water were added thereto. The organic layer was separated, then dried over anhydrous sodium sulfate, and then purified by silica gel column chromatography (ethyl acetate/methanol=100/5) to obtain compound (Y6) (1.47 g). TLC Rf: 0.51 (dichloromethane/methanol=9/1)
(7) Compound (Y6) (225 mg), methanol (10 mL), and 10% Pd/C (62 mg) were placed in a sealed tube and stirred for 17 hours in a hydrogen atmosphere. Insoluble matter was filtered, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7:3:0.5) to obtain compound (Y7) (126 mg). TLC Rf: 0.69 (chloroform/ethanol/ammonia water=7/3/0.5)
(8) A mixture of disodium Fmoc-cysteinate (40 mg), DMF (0.5 mL), and methanesulfonic acid (7.2 μL) was stirred at room temperature for 30 minutes, and then, DIEA (77 μL), DMF (1.5 mL), and compound (Y7) (69 mg) were added thereto. HBTU (48 mg) was added thereto, and the mixture was stirred at room temperature for 2.5 hours, followed by the addition of chloroform and water. The organic layer was separated, then dried over anhydrous sodium sulfate, and purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (Y8) (76 mg). TLC Rf: 0.35 (chloroform/ethanol/ammonia water=7/3/0.5)
(9) A mixture of compound (Y8) (140 mg), DMF (2 mL), and diethylamine (200 μL) was stirred at room temperature for 80 minutes. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (Y9) (100 mg). TLC Rf: 0.10 (chloroform/ethanol/ammonia water=7/3/0.5)
(10) A mixture of disodium Fmoc-cysteinate (46 mg), DMF (1 mL), and methanesulfonic acid (8.3 μL) was stirred at room temperature for 30 minutes. Then, DIEA (89 μL), DMF (1.5 mL), compound (Y9) (100 mg), and HBTU (58 mg) were added thereto, and the mixture was stirred at room temperature for 1.5 hours. Chloroform and water were added to the reaction mixture. The organic layer was separated, then dried over anhydrous sodium sulfate, and purified by silica gel column chromatography (chloroform/ethanol/ammonia water=6/4/1) to obtain compound (Y10) (155 mg). TLC Rf: 0.19 (chloroform/ethanol/ammonia water=6/4/1)
(11) A mixture of compound (Y10) (142 mg), DMF (2 mL), and diethylamine (200 μL) was stirred at room temperature for 1 hour. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=5/5/1.5) to obtain compound (Y11) (89 mg). TLC Rf: 0.21 (chloroform/ethanol/ammonia water=5/5/1.5) MS (ESI, m/z): 1013.3 [M−BOC+2Na]+, 1067.3 [M−H]−, 533.2 [M−2H]2−
(12) To a mixture of compound (Y11) (26 mg), tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (16 mg), DMF (500 μL), and DIEA (21 μL), HATU (23 mg) was added, and the resulting mixture was stirred at room temperature for 5 minutes. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (chloroform/ethanol/ammonia water=7/3/0.5) to obtain compound (Y12) (25 mg). TLC Rf: 0.22 (chloroform/ethanol/ammonia water=7/3/0.5)
(13) To compound (Y12) (45 mg), TFA (2 mL) was added, and the mixture was stirred at room temperature for 22 hours. The solvent was distilled off under reduced pressure. To the obtained residue, DMF (2 mL) and sodium hydroxide (17 mg) were added, and the mixture was stirred at room temperature for 47 hours. The solvent was distilled off under reduced pressure, and the residue was purified on a reversed-phase silica gel (Sep-Pak C18, water/methanol=5/95 to 10/90) to obtain compound (Y13) (40 mg). Reversed-phase TLC Rf: 0.68 (water/acetonitrile=95/5)
(1) A solution of 2-(tert-butoxycarbonylamino)pyridine (1.07 g) in DMF (6 mL) was ice-cold. Sodium hydride (60% dispersion in mineral oil, 221 mg) was added thereto over 10 minutes, and the mixture was stirred at the same temperature as above for 15 minutes and then added to a solution of ethyl 4-(3-bromopropyl)benzoate (1.5 g) in DMF (6 mL), followed by stirring at room temperature for 3 hours. The reaction mixture was added to 2% hydrochloric acid, followed by extraction with ethyl acetate. The extract was washed with a saturated aqueous solution of sodium carbonate and a saturated aqueous solution of sodium chloride in this order. The resultant was dried over anhydrous magnesium sulfate, and the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate) to obtain compound (Z1) (1.8 g). 1H-NMR (300 MHz, CDCl3) δ: 8.37 (1H, dd, J=2.1, 4.2 Hz), 7.94 (2H, d, J=7.2 Hz), 7.54-7.65 (2H, m), 7.22 (2H, d, J=7.2 Hz), 6.99-7.03 (1H, m), 4.36 (2H, q, J=7.2 Hz), 3.98 (2H, t, J=7.2 Hz), 2.69 (2H, t, J=7.2 Hz), 1.93-2.03 (2H, m), 1.48 (9H, s), 1.38 (3H, t, J=7.2 Hz)
(2) To compound (Z1) (1.5 g), concentrated hydrochloric acid (5 mL) was added, and the mixture was stirred at 70° C. for 3 hours. Concentrated hydrochloric acid (2 mL) was added thereto, and the mixture was stirred at room temperature for 12 hours. A saturated aqueous solution of sodium bicarbonate was added thereto until the pH reached 4, followed by extraction with ethyl acetate. The solvent was distilled off under reduced pressure to obtain compound (Z2) (300 mg). LC/MS (ACQUITY) rt (min): 0.66 MS (ESI, m/z): 257.1 [M+H]+
(3) To a mixed solution of compound (A2) (750 mg), compound (Z2) (200 mg), DMF (3 mL), and DIEA (0.68 mL), HBTU (296 mg) was added, and the mixture was stirred at room temperature for 1 hour. Ethyl acetate and water were added thereto. The organic layer was separated and dried over anhydrous magnesium sulfate, and then, the solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (chloroform/methanol) to obtain compound (Z3) (480 mg). LC/MS (ACQUITY) rt (min): 1.16 MS (ESI, m/z): 803.5 [M+H]+
(4) Compound (Z3) (480 mg), methanol (30 mL), and 10% Pd/C (100 mg) were placed in a sealed tube and stirred for 5 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (Z4) (510 mg).
(5) A mixture of disodium Fmoc-cysteinate (73.4 mg), THF (1 mL), and methanesulfonic acid (11 μL) was stirred at room temperature for 30 minutes. To the reaction mixture, DIEA (29 μL), NMP (1 mL), and compound (Z4) (94 mg) were added, then HBTU (64 mg) was added, and the mixture was stirred at room temperature for 2.5 hours. Methanol was added thereto. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The residue was washed twice with water. THF (2 mL), NMP (0.3 mL), and diethylamine (2 mL) were added thereto, and the mixture was stirred at room temperature for 3 hours. The solvent was distilled off under reduced pressure, and the residue was washed with toluene to obtain compound (Z5) (74 mg). LC/MS (ACQUITY) rt (min): 0.78 MS (ESI, m/z): 820.4 [M+H]+
(6) A mixture of disodium Fmoc-cysteinate (45.9 mg), THF (2 mL), and methanesulfonic acid (6.8 μL) was stirred at room temperature for 1 hour. Then, compound (Z5) (74 mg), NMP (0.7 mL), DIEA (18 μL), and HBTU (40.0 mg) were added thereto, and the mixture was stirred at room temperature for 2.5 hours. Methanol was added thereto. The solvent was distilled off under reduced pressure, and the residue was washed with ethyl acetate. NMP (1 mL) and diethylamine (1 mL) were added to the residue, and the mixture was stirred at room temperature for 3 hours. The solvent was distilled off under reduced pressure. The obtained residue was purified by preparative HPLC to obtain compound (Z6) (6.7 mg). LC/MS (ACQUITY) rt (min): 0.83 MS (ESI, m/z): 971.5 [M+H]+, 486.4 [M+2H]2+, 969.5 [M−H]−
(7) To a solution of tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (5.9 mg) and compound (Z6) (6.7 mg) in NMP (200 μL) and DIEA (20 μL), HBTU (5.2 mg) was added, and the mixture was stirred at room temperature for 50 minutes. Methanol (100 μL) was added thereto, and then, the mixture was purified by preparative HPLC to obtain compound (Z7) (3.9 mg). LC/MS (ACQUITY) rt (min): 1.08 MS (ESI, m/z): 763.9 [M+2H]2+
(8) A mixture of compound (Z7) (3.9 mg), THF (200 μL), water (20 μL), 2-propanol (20 μL), and a 4 mol/L aqueous lithium hydroxide solution (27 μL) was stirred at room temperature for 4 hours. TFA was added thereto, and the solvent was distilled off under reduced pressure. TFA/triethylsilane (95/5) (100 μL) was added to the residue, and the mixture was stirred for 2 hours. Then, the solvent was distilled off under reduced pressure. The obtained residue was purified by preparative HPLC to obtain compound (Z8) (1.8 mg). LC/MS (ACQUITY) rt (min): 0.76 MS (ESI, m/z): 672.6 [M+2H]2+, 670.6 [M−2H]2−
(1) To a mixture of L-glutamic acid γ benzyl ester (5.0 g), water (10 mL), sodium bromide (7.6 g), and hydrobromic acid (6 mL), sodium nitrite (2.6 g) was added at 5° C. or lower over 10 minutes, and the resulting mixture was stirred at 5° C. for 2 hours. Diisopropyl ether and concentrated sulfuric acid (2 mL) were added to the reaction mixture. The organic layer was separated, then washed with water and a saturated aqueous solution of sodium chloride in this order, and then dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. Then, the obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=1/1) to obtain compound (Aa1) (3.1 g). LC/MS (ACQUITY) rt (min): 1.32 MS (ESI, m/z): 301.1 [M+H]1H-NMR (300 MHz, CDCl3) δ: 7.31-7.38 (5H, m), 5.1 (2H, s), 4.41 (1H, dd, J=6.0, 7.8 Hz), 2.58-2.63 (2H, m), 2.25-2.50 (2H, m)
(2) To a solution of compound (Aa1) (3.1 g) in chloroform (15 mL), a mixture of tert-butyl 2,2,2-trichloroacetimidate (4.3 mL) and hexane (12 mL) was added at room temperature over 20 minutes. DMAc (1.5 mL) and BF3.OEt2 (220 μL) were added thereto, and the mixture was stirred at room temperature for 40 hours. Then, the solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (hexane/ethyl acetate=95/5 to 85/15) to obtain compound (Aa2) (2.84 g). 1H-NMR (300 MHz, CDCl3) δ: 7.31-7.38 (5H, m), 5.14 (2H, s), 4.24 (1H, dd, J=6.0, 8.7 Hz), 2.53-2.59 (2H, m), 2.19-2.43 (2H, m), 1.47 (9H, a)
(3) To a solution of 1,4,8,11-tetraazacyclotetradecane (1.84 g) in chloroform (60 mL), a solution of compound (Aa2) (1.70 g) in chloroform (50 mL) was added over 90 minutes, and the mixture was stirred at room temperature for 3 days. The solvent was distilled off under reduced pressure, and the residue was purified by silica gel column chromatography (hexane/ethyl acetate=50/50 to 0/100 and then ethyl acetate/methanol=80/20) to obtain compound (Aa3) (0.76 g). LC/MS (ACQUITY) rt (min): 0.91 MS (ESI, m/z): 406.5 [M+H]+
(4) To a mixture of compound (Aa3) (0.76 g), DMAc (7 mL), and potassium carbonate (607 mg), tert-butyl bromoacetate (580 μL) was added, and the resulting mixture was stirred at room temperature for 2 hours. Ethyl acetate (30 mL) and water (30 mL) were added thereto. The organic layer was separated, then washed twice with water (30 mL) and once with a saturated aqueous solution of sodium chloride (30 mL) in this order, and dried over anhydrous sodium sulfate. The solvent was distilled off under reduced pressure. The obtained residue was purified by silica gel column chromatography (hexane/ethyl acetate=95/5 to 60/40) to obtain compound (Aa4) (1.04 g). LC/MS (ACQUITY) rt (min): 1.63 MS (ESI, m/z): 634.7 [M+H]+
(5) Compound (Aa4) (0.28 g), isopropyl alcohol (20 mL), water (0.5 mL), and 10% Pd/C (0.10 g) were placed in a sealed tube and stirred for 7 hours in a 0.5 MPa hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (Aa5) (0.24 g). LC/MS (ACQUITY) rt (min): 1.34 MS (ESI, m/z): 544.7 [M+H]+
(6) To a mixture of compound (Aa5) (94.9 mg), (R)-2-amino-3-((2-(4-(4-(N—((S)-1-methoxy-1-oxo-3-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)propan-2-yl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-3-oxopropane-1-sulfonic acid (104 mg), DMF (0.8 mL), and N,N-diisopropylethylamine (61 μL), HBTU (64.5 mg) was added, and the resulting mixture was stirred at room temperature for 35 minutes. Water (1.1 mL) and acetonitrile (0.8 mL) were added thereto, and the mixture was stirred and then purified by preparative HPLC to obtain compound (Aa6) (151 mg). HPLC (CAPCELL PAK MG) rt (min): 11.82 LC/MS (ACQUITY) rt (min): 1.28 MS (ESI, m/z): 1324.2 [M+H]+, 1322.2 [M−H]−
(7) To compound (Aa6) (73 mg), concentrated hydrochloric acid (2.5 mL) was added, and the mixture was stirred at room temperature for 2 days and then concentrated under reduced pressure. The concentrate was diluted with acetonitrile containing 50% water (2 mL) and then purified by preparative HPLC to obtain compound (Aa7) (33.3 mg). HPLC (CAPCELL PAK MG) rt (min): 9.37 LC/MS (ACQUITY) rt (min): 0.77 MS (ESI, m/z): 1141.8 [M+H]+, 1139.8 [M−H]−
(1) To a solution of 1,4,8,11-tetraazacyclotetradecane (5.35 g) in acetonitrile (450 mL), 39% glyoxal (4.5 mL) was added, and the mixture was stirred at room temperature for 1.5 hours and then stirred at 50° C. for 2 hours. The solvent was distilled off under reduced pressure. Diisopropyl ether (100 mL) was added to the residue, and the mixture was stirred at room temperature for 1 hour. Then, the obtained solid was collected by filtration to obtain compound (Ab1) (2.46 g). 1H-NMR (300 MHz, CDCl3) δ: 3.49-3.57 (2H, m), 3.08 (2H, s), 2.93-2.97 (6H, m), 2.74 (2H, d, J=11.1 Hz), 2.01-2.35 (8H, m), 1.19-1.26 (2H, m)
(2) To a solution of compound (Ab1) (2.40 g) in acetonitrile (40 mL), benzyl bromide (18 mL) was added, and the mixture was stirred at room temperature for 15 days. The deposited solid was collected by filtration and washed with acetonitrile and dichloromethane to obtain compound (Ab2) (4.0 g). LCMS (ACQUITY) rt (min): 0.46 MS (ESI, m/z): 313.4 [M−Bn]+
(3) To a mixture of compound (Ab2) (4.0 g), ethanol (180 mL), and water (9 mL), sodium borohydride (4 g) was added in 4 divided portions every 15 minutes, and the resulting mixture was stirred at room temperature for 3 days. After addition of 3M hydrochloric acid (80 mL) and water (100 mL) in this order under cooling, the reaction mixture was neutralized with sodium hydroxide, followed by extraction with toluene (200 mL) twice. The extract was dried over anhydrous sodium sulfate, and then, the solvent was distilled off under reduced pressure to obtain compound (Ab3) (1.9 g). LC/MS (ACQUITY) rt (min): 0.73 MS (ESI, m/z): 407.6 [M+H]+
(4) Compound (Ab3) (0.90 g), acetic acid (25 mL), and 10% Pd/C (230 mg) were placed in a sealed tube and stirred for 11 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. Then, water (30 mL), sodium hydroxide (2 g), and a saturated aqueous solution of sodium chloride (10 mL) were added to the residue, followed by extraction with toluene (50 mL) three times. The solvent was distilled off under reduced pressure to obtain compound (Ab4) (509 mg). LC/MS (ACQUITY) rt (min): 0.20 MS (ESI, m/z): 227.4 [M+H]+ 1H-NMR (300 MHz, CDCl3) δ: 3.73 (1H, brs), 3.11 (2H, ddd, J=2.7, 9.9, 13.2 Hz), 2.59-2.94 (14H, m), 2.34-2.46 (4H, m), 2.26 (1H, brs), 1.85-1.99 (2H, m), 1.25-1.36 (2H, m)
(5) To a mixture of compound (Ab4) (509 mg), acetonitrile (8 mL), and potassium carbonate (930 mg), a solution of compound (Aa2) (880 mg) in acetonitrile (3 mL) was added, and the resulting mixture was stirred at room temperature for 1 day. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (ethyl acetate/isopropylamine=100/5 to 100/10) to obtain compound (Ab5) (467 mg). LC/MS (ACQUITY) rt (min): 0.92 MS (ESI, m/z): 503.6 [M+H]+
(6) To a mixture of compound (Ab5) (383 mg), DMAc (3 mL), and potassium carbonate (250 mg), tert-butyl bromoacetate (123 HL) was added, and the resulting mixture was stirred at room temperature for 2 hours. Ethyl acetate (20 mL), water (10 mL), and a saturated aqueous solution of sodium chloride (20 mL) were added thereto. The organic layer was separated and dried over anhydrous sodium sulfate, and the solvent was distilled off under reduced pressure. The residue was purified by silica gel column chromatography (chloroform/methanol=9/1, ethyl acetate/isopropylamine=10/1) and preparative HPLC in this order to obtain compounds (Ab6-a) (76 mg) and (Ab6-b) (50 mg) ((Ab6-a) and (Ab6-b) were stereoisomers). (Ab6-a) LC/MS (ACQUITY) rt (min): 1.19 MS (ESI, m/z): 617.7 [M+H]+ (Ab6-b) LC/MS (ACQUITY) rt (min): 1.64 MS (ESI, m/z): 617.7 [M+H]+
(7-a) Compound (Ab6-a) (76 g), THF (2 mL), water (2 mL), and 10% Pd/C (10 mg) were placed in a sealed tube and stirred for 6 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (Ab7-a) (64 mg). LC/MS (ACQUITY) rt (min): 0.82 MS (ESI, m/z): 527.5 [M+H]+
(7-b) Compound (Ab6-b) (50 g), THF (2 mL), water (2 mL), and 10% Pd/C (10 mg) were placed in a sealed tube and stirred for 6 hours in a hydrogen atmosphere. Insoluble matter was filtered off, and the solvent was distilled off under reduced pressure to obtain compound (Ab7-b) (45 mg). LC/MS (ACQUITY) rt (min): 1.30 MS (ESI, m/z): 527.5 [M+H]+
(8-a) To a mixture of compound (Ab7-a) (60.9 mg), (R)-2-amino-3-((2-(4-(4-(N—((S)-1-methoxy-1-oxo-3-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)propan-2-yl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-3-oxopropane-1-sulfonic acid (92.6 mg), DMF (0.8 mL), and N,N-diisopropylethylamine (50 μL), HBTU (48.4 mg) was added, and the resulting mixture was stirred at room temperature for 15 minute. Water (0.5 mL) and a 50% aqueous acetonitrile solution (0.6 mL) were added thereto, and the mixture was stirred and then purified by preparative HPLC to obtain compound (Ab8-a) (74 mg). HPLC (CAPCELL PAK MG) rt (min): 9.74 LC/MS (ACQUITY) rt (min): 0.91 MS (ESI, m/z): 1307.0 [M+H]+, 654.3 [M+2H]2+, 1305.0 [M−H]−
(8-b) To a mixture of compound (Ab7-b) (40.2 mg), (R)-2-amino-3-((2-(4-(4-(N—((S)-1-methoxy-1-oxo-3-(5-(5,6,7,8-tetrahydro-1,8-naphthyridin-2-yl)pentanamido)propan-2-yl)sulfamoyl)-3,5-dimethylphenoxy)butanamido)ethyl)amino)-3-oxopropane-1-sulfonic acid (60.6 mg), DMF (0.8 mL), and N,N-diisopropylethylamine (35 μL), HBTU (31.7 mg) was added, and the resulting mixture was stirred at room temperature for 15 minutes. Water (0.5 mL) and a 50% aqueous acetonitrile solution (0.6 mL) were added thereto, and the mixture was stirred and then purified by preparative HPLC to obtain compound (Ab8-b) (69 mg). HPLC (CAPCELL PAK MG) rt (min): 11.88 LC/MS (ACQUITY) rt (min): 1.22 MS (ESI, m/z): 1307.0 [M+H]+, 654.3 [M+2H]2+, 1305.1 [M−H]−
(9-a) To compound (Ab8-a) (69.5 mg), concentrated hydrochloric acid (2.5 mL) was added, and the mixture was stirred at room temperature for 2 days and then concentrated under reduced pressure. The concentrate was diluted with acetonitrile containing 50% water (2.4 mL) and then purified by preparative HPLC to obtain compound (Ab9-a) (44.1 mg). HPLC (CAPCELL PAK MG) rt (min): 8.95 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1180.8 [M+H]+, 590.9 [M+2H]2+, 1178.8 [M−H]−
(9-b) To compound (Ab8-b) (64.4 mg), concentrated hydrochloric acid (2.5 mL) was added, and the mixture was stirred at room temperature for 2 days and then concentrated under reduced pressure. The concentrate was diluted with acetonitrile containing 50% water (2.4 mL) and then purified by preparative HPLC to obtain compound (Ab9-b) (33.1 mg). HPLC (CAPCELL PAK MG) rt (min): 9.19 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1180.8 [M+H]+, 591.0 [M+2H]2+, 1178.7 [M−H]−
To a mixture of compound (P2) (112 mg), water (1 mL), a 1 mol/L aqueous ammonium acetate solution (1 mL), and acetic acid (300 μL), a solution of indium chloride tetrahydrate (129 mg) in water (0.5 mL) was added, and the resulting mixture was stirred at 110° C. for 10 minutes. A 50% aqueous acetonitrile solution (3 mL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (AA) (118 mg). HPLC (CAPCELL PAK MG) rt (min): 9.35 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1280.9 [M−H]−
(2)
To a mixture of compound (D3) (2.4 mg), water (80 μL), a 0.5 mol/L aqueous ammonium acetate solution (100 μL), acetic acid (10 μL), and gentisic acid (0.4 mg), a mixture (50 μL) of indium chloride tetrahydrate (15.6 mg) and water (156 μL) was added, and the resulting mixture was heated at 100° C. for 10 minutes. A 50% aqueous acetonitrile solution (500 μL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (BB) (1.6 mg). HPLC (SunFire) rt (min): 7.89 LC/MS (SunFire) rt (min): 7.76 MS (ESI, m/z): 662.75 [M+2H]2+, 442.15 [M+3H]3+
(3)
Compound (CC) (1.2 mg) was obtained in the same way as in Example 29(1) using compound (J9) (1.3 mg). HPLC (SunFire) rt (min): 10.93 LC/MS (SunFire) rt (min): 10.25 MS (ESI, m/z): 686.30 [M+2H]2+, 684.15 [M−2H]2−
To a mixture of compound (P2) (140 mg), water (1 mL), a 1 mol/L aqueous ammonium acetate solution (1 mL), and acetic acid (300 μL), a solution of yttrium chloride hexahydrate (143 mg) in water (0.5 mL) was added, and the resulting mixture was stirred at 110° C. for 10 minutes. A 50% aqueous acetonitrile solution (3 mL) was added thereto, and the mixture was purified by preparative HPLC to obtain compound (DD) (111 mg). HPLC (CAPCELL PAK MG) rt (min): 9.71 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1256.6 [M+H]+, 1254.6 [M−H]−
A mixture of compound (P2) (36.8 mg), a 0.5 mol/L aqueous sodium acetate solution/water/acetic acid (10/10/1) (1.2 mL), and copper(II) chloride (4.4 mg) was stirred at 110° C. for 10 minutes. The reaction mixture was purified on SepPak C18 (water/methanol=1/1) to obtain compound (EE) (39.1 mg). HPLC (MG) rt (min): 9.23 LC/MS (ACQUITY) rt (min): 0.75 MS (ESI, m/z): 1231.6 [M+H]+, 1229.6 [M−H]−
A mixture of compound (Aa7) (16.9 mg), a 0.5 mol/L aqueous sodium acetate solution/water/acetic acid (10/10/1) (0.4 mL), and copper(II) chloride (3.2 mg) was stirred at 110° C. for 5 minutes and then purified on SepPak C18 (water/methanol=1/1) to obtain compound (FF) (14.4 mg). HPLC (CAPCELL PAK MG) rt (min): 9.80 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 1202.5 [M+H]+, 601.9 [M+2H]2+, 1200.5 [M−H]−
A mixture of compound (Ab9-a) (16.2 mg), a 0.5 mol/L aqueous sodium acetate solution/water/acetic acid (80/80/1) (0.4 mL), and copper(II) chloride (3.1 mg) was stirred at 110° C. for 10 minutes and then purified by preparative HPLC to obtain compound (GG) (16.0 mg). HPLC (CAPCELL PAK MG) rt (min): 9.52 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 1241.7 [M+H]+, 621.5 [M+2H]2+, 1239.7 [M−H]−
A mixture of compound (Ab9-b) (13.6 mg), a 0.5 mol/L aqueous sodium acetate solution/water/acetic acid (80/80/1) (0.4 mL), and copper(II) chloride (3.1 mg) was stirred at 110° C. for 10 minutes and then purified by preparative HPLC to obtain compound (HH) (13.3 mg). HPLC (CAPCELL PAK MG) rt (min): 9.59 LC/MS (ACQUITY) rt (min): 0.79 MS (ESI, m/z): 1241.7 [M+H]+, 621.5 [M+2H]2+, 1239.7 [M−H]−
To a mixed solution of compound (P2) (8.5 μg) and a 0.2 mol/L sodium acetate buffer solution (pH 4.0) (1.5 mL), a [111In] indium chloride solution (80 MBq, 100 μL) was added. The mixture was heated at 100° C. for 15 minutes and then left at room temperature for 5 minutes to obtain radiolabeled compound [111In]-(P2). As a result of analysis by reversed-phase TLC (Whatman, KC18F, development solvent: methanol/0.5 mol/L aqueous ammonium acetate solution (50/50)); the radiolabeled compound had an Rf value of 0.4. Its radiochemical purity was 95% or more both immediately after preparation and after standing at room temperature for 24 hours.
To a mixed solution of compound (P2) (79 μg), gentisic acid (1.8 mg), a 0.6 mol/L sodium acetate buffer solution (pH 4.0, 120 μL), and a 0.4 mol/L aqueous sodium hydroxide solution (24 μL), a [90Y] yttrium chloride solution (700 MBq, 240 μL) was added. The mixture was heated at 100° C. for 20 minutes and then left at room temperature for 5 minutes to obtain radiolabeled compound [90Y]-(P2). As a result of analysis by reversed-phase TLC (Whatman, KC18F, development solvent: methanol/0.5 mol/L aqueous ammonium acetate solution (50/50)), the radiolabeled compound had an Rf value of 0.4. Its radiochemical purity was 95% or more both immediately after preparation and after standing at room temperature for 24 hours.
To a mixed solution of compound (P2) (5.8 g) and a 0.2 mol/L sodium acetate buffer solution (pH 4.0, 219 μL), a [64Cu] copper chloride solution (pH 5, 35 MBq, 55 μL) was added. The mixture was heated at 100° C. for 15 minutes and then left at room temperature for 5 minutes to obtain radiolabeled compound [64Cu]-(P2). As a result of analysis by reversed-phase TLC (Whatman, KC18F, development solvent: methanol/0.5 mol/L aqueous ammonium acetate solution (50/50)), the radiolabeled compound had an Rf value of 0.4. Its radiochemical purity was 90% or more both immediately after preparation and after standing at room temperature for 22 hours.
Radiolabeled compounds were synthesized in the same way as in (1) and (2).
To a mixed solution of compound (Aa7) (4.2 μg), gentisic acid (1 mg), and a 0.2 mol/L sodium acetate buffer solution (pH 4.0) (5.0 μL), [64Cu] copper chloride in a 0.2 mol/L sodium acetate buffer solution (pH 4.0) (40 MBq, 155 μL) was added. The mixture was heated at 100° C. for 15 minutes and then left at room temperature for 5 minutes to obtain radiolabeled compound [64Cu]-(Aa7). As a result of analysis by reversed-phase TLC (Merck KGaA, RP-8 F254s, development solvent: methanol/0.5 mol/L aqueous ammonium acetate solution (50/50)), the radiolabeled compound had an Rf value of 0.4. Its radiochemical purity was 90% or more both immediately after preparation and after standing at room temperature for 24 hours.
Radiolabeled compounds were synthesized in the same way as in (28).
The results about (4) to (30) are shown below.
0.2 g/mL αvβ3 (Chemicon International, Inc.) was immobilized on each well of a 96-well plate (Corning Inc.). Each well was blocked with a 1% Block Ace (DS Pharma Biomedical Co., Ltd) solution and then washed with T-PBS (PBS containing 0.05% Tween 20). Evaluation compound solutions having a 2-fold concentration (10 concentrations of 3.16-fold dilutions from 0.3 μmol/L, buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, and 1 mM MnCl2)) and a 4 μg/mL biotinylated vitronectin solution (vitronectin (Upstate Biotechnology Inc.) was labeled with EZ-Link Sulfo-NHS-Biotinylation Kit (Pierce/Thermo Fisher Scientific Inc.) and then concentration-adjusted) were each added at 50 μL/well, and the plate was shaken at room temperature for 2 hours. After washing with T-PBS, a 0.2 μg/mL avidin peroxidase (Pierce/Thermo Fisher Scientific Inc.) solution was added thereto, and the plate was shaken at room temperature for 1 hour. After washing with T-PBS, color was developed by an o-phenylenediamine (Sigma-Aldrich Inc.) solution (the reaction was terminated by the addition of 4 mol/L sulfuric acid), and the absorbance was measured (490 nm, Reference: 595 nm). The IC50 value was calculated using XLfit 3.0 (ID Business Solutions Ltd.). RGDfV (Bachem AG) was measured in duplicate as a QC sample for each plate.
0.2 g/mL αvβ5 (Chemicon International, Inc.) was immobilized on each well of a 96-well plate (Corning Inc.). Each well was blocked with a 1% Block Ace (DS Pharma Biomedical Co., Ltd) solution and then washed with PBST (10 mM Na2HPO4 pH 7.5, 150 mM NaCl, and 0.01% Tween 20). Evaluation compound solutions having a 2-fold concentration (10 concentrations of 3.16-fold dilutions from 0.3 μmol/L, buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, and 1 mM MnCl2)) and a 4 μg/mL biotinylated vitronectin solution (vitronectin (Upstate Biotechnology Inc.) was labeled with EZ-Link Sulfo-NHS-Biotinylation Kit (Pierce/Thermo Fisher Scientific Inc.) and then concentration-adjusted) were each added at 50 μL/well, and the plate was shaken at room temperature for 2 hours. After washing with PBST, a 0.2 μg/mL avidin peroxidase (Pierce/Thermo Fisher Scientific Inc.) solution was added thereto, and the plate was shaken at room temperature for 1 hour. After washing with PBST, color was developed by an o-phenylenediamine (Sigma-Aldrich Inc.) solution (the reaction was terminated by the addition of 4 mol/L sulfuric acid), and the absorbance was measured (490 nm, Reference: 595 nm). The IC50 value was calculated using XLfit 3.0 (ID Business Solutions Ltd.). RGDfV (Bachem AG) was measured in duplicate as a QC sample for each plate.
The results of Test Example 1 and Test Example 2 are shown below.
The compounds shown in Table 3 exhibited excellent integrin binding affinity.
The integrin-specific accumulation of an 111In-labeled compound to a tumor of a subcutaneously integrin-expressing cell-transplanted mouse was confirmed by tissue extraction and radioactivity measurement methods.
1. Confirmation of Integrin Expression in Tumor Cell Used in Experiment
The integrin expression levels of tumor masses in which an A375 (human melanoma), A498 (human renal cell cancer), HCT116 (human colorectal cancer), U87MG (human glioblastoma), or T98G (human glioblastoma) cell line was subcutaneously transplanted were confirmed by Western blotting. Cultured cells of each line were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc., male, 6 to 8 weeks old). 2 to 12 weeks after transplantation, a tumor was extracted from each mouse. The extracted tumor was minced with scissors and then prepared into a homogenate by using a homogenizer. The protein level of each sample was adjusted to 1 mg/mL (1×Tris/glycine/SDS+100 mM DTT buffer). Integrins αvβ3 (R&D Systems, Inc., 3050-AV) and αvβ5 (Chemicon International, Inc., CC1024) were each adjusted to 4 concentrations (1, 2, 5, and 10 ng/well) as standards. These proteins were simultaneously separated by SDS-PAGE (10% gel; manufactured by Bio Craft Co., Ltd.). After the separation, the proteins were transferred to PVDF membranes, which were then blocked with a blocking solution (5% skimmed milk/PBS-T) for 1 hour and then washed twice with PBS-T. After reaction with each of an anti-integrin β3 antibody (Cell Signaling Technology, Inc., #4702), an anti-integrin β5 antibody (Santa Cruz Biotechnology, Inc., SC-5402), and anti-β-actin antibody (Sigma-Aldrich Inc., A5441) as primary antibodies, the membranes were washed three times with PBS-T. ECL Anti-Rabbit IgG horseradish Peroxidase (GE Healthcare Japan Corp., NA934V), ECL Anti-mouse IgG horseradish Peroxidase (GE Healthcare Japan Corp., NA931V), or Donkey Anti-goat antibody HRP conjugate (Bethyl Laboratories, Inc., A50-101P) was used as a secondary antibody in reaction, and the membranes were washed three times with PBS-T. Light was developed using a chemiluminescent reagent (Super Signal West Femto Maximum Sensitivity Substrate; Thermo Fisher Scientific Inc., 34096) and measured using LAS3000 (GE Healthcare Japan Corp.). The integrin expression level per μg of tumor mass was calculated from the standards. The results are shown below.
2. Confirmation of Integrin-Specific Accumulation of [111In]-(A8) by Tissue Extraction and Radioactivity Measurement Methods
SK-MEL-28 (human melanoma), A375 (human melanoma), A498 (human renal cell cancer), Caki-2 (human renal cell cancer), HCT116, U87MG, and T98G cells were studied as follows: the cells of each line were cultured and subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc., male, 6 to 8 weeks old). The mice were raised until their tumor volumes reached 85 to 1,075 mm3. Then, the mice were allocated at the time of anatomy to groups each involving 3 individuals so as to prevent the tumor volumes from disproportioning among the groups. Then, the radiolabeled compound [111In]-(A8) (740 kBq) was administered to the tail veins of the mice. The animals were sacrificed at the time of anatomy to extract their tumors. The weights of the tumors were measured, and then, the radioactivity was measured using a gamma counter to calculate the concentration of radioactivity in the tumors (% ID/g: % injected dose/g). The results are shown below.
The integrin expression level of the tumor masses differed among the cell lines and was 1.54 to 8.28 ng/μg for β3 and 1.99 to 13.51 ng/μg for β5. The tumor accumulation of [111In]-(A8) 4 hours and 24 hours after administration differed among the cell lines and was 3.1 to 13.1% ID/g 4 hours later and 2.9 to 9.3% ID/g 24 hours later. Furthermore, the strong correlation (R=0.827) was confirmed between the expression level of integrin β3 in the tumor masses and the accumulation of radioactivity at the tumors 24 hours after administration (
U87MG cells were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc. or Japan SLC, Inc., 6 to 9 weeks old). After 2 to 3 weeks, the mice were divided into groups each involving 3 individuals per point in time when their tumors became 200 to 500 mm3. The 111In-labeled compound (740 kBq) was administered to the tail veins of the mice.
After a given time, the animals were sacrificed to extract their tumors. The weights of the tumors were measured, and the radioactivity was measured using a gamma counter to calculate the concentration of radioactivity in the tumors (% ID/g). In the same way as above, the concentration of radioactivity in the tumors (% ID/g) was calculated for the 64Cu-labeled compound (500 kBq) and the 90Y-labeled compound (500 kBq). The results are shown below.
The concentrations of radioactivity in the tumors of the compounds shown in Table 5 were 6.95 to 12.80% ID/g 4 hours after administration and 3.34 to 15.29% ID/g 24 hours after administration.
U87MG cells were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc. or Japan SLC, Inc., male, 6 to 9 weeks old). After 2 weeks, the radiolabeled compound [64Cu]-(P2) was administered at 4.8 MBq/mouse to the tail veins of mice whose tumors became 250 to 650 mm3. After 1, 4, 24, and 48 hours, the images were taken in microPET/CT (Inveon, Siemens AG) under isoflurane anesthesia. After the imaging at 48 hours after administration, the mice were euthanized by the collection of the whole blood from the postcava under deep anesthesia with isoflurane, followed by tumor extraction. The weights of the tumors were measured, and the radioactivity was measured using a gamma counter to calculate the concentration of radioactivity in the tumors (% ID/g). In the same way as above, [64Cu]-(Aa7), [64Cu]-(Ab9-a), and [64Cu]-(Ab9-b) were imaged. However, the concentration of radioactivity in the tumors was not calculated. The PET images of each compound at each time point are shown in
U87MG cells were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc., male, 6 weeks old). After 2 weeks, a dosing solution of the radiolabeled compound [111In]-(P2) was administered at 1 MBq/mouse to the tail veins of mice whose tumors became 300 to 600 mm3. 24, 48, and 72 hours after administration, the planar images were taken with a gamma camera (Symbia, Siemens AG) under isoflurane anesthesia. The radioactivity in the tumors (% ID) was calculated by image analysis.
U87MG cells were intracranially transplanted at 1×107 cells/mouse to Balb/cAJcl-nu/nu (CLEA Japan, Inc., male, 6 weeks old) using a tapered needle. After 2 to 4 weeks, a dosing solution of the radiolabeled compound [111In]-(P2) was administered at 1 MBq/mouse to the tail veins of the mice. 24, 48, and 72 hours after administration, the planar images were taken with a gamma camera (Symbia, Siemens AG) under isoflurane anesthesia (
U87MG cells were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/c Slc-nu/nu (SLC Japan, Inc., male, 6 weeks old). After 2 weeks, mice whose tumors became 100 to 500 mm3 were grouped. Phosphate-buffered saline (PBS) or the radiolabeled compound [90Y]-(P2) was administered to the tail veins of the mice, and their tumor volumes were measured. When the tumor volumes of the mice in the PBS group exceeded 2,000 mm3, which is a humanistic endpoint, the antitumor effect was evaluated. The evaluation values were the rate of inhibition of tumor growth ((1−(Average tumor volume of the compound administration group−Average tumor volume of the compound administration group before administration)/(Average tumor volume of the PBS group−Average tumor volume of the PBS group before administration))×100 (provided that the rate of inhibition exceeding 100% was indicated as 100%)) and the number of individuals having a tumor volume equal to or smaller than that at the start of the experiment (the number of individuals having tumor regression). The results are shown below.
The compound shown in Table 6 exhibited an excellent antitumor effect.
Subcutaneously U87MG-Transplanted Model Using [90Y]-(A8)
U87MG cells were subcutaneously transplanted at 1×107 cells/mouse to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc., male, 6 weeks old). After 2 weeks, mice whose tumors became 100 to 500 mm3 were grouped. Phosphate-buffered saline (PBS) or the radiolabeled compound [90Y]-(A8) was administered to the tail veins of the mice, and their tumor volumes were measured. The evaluation values were calculated in the same way as in Test Example 8 to evaluate the antitumor effect. The results are shown below.
The compound shown in Table 7 exhibited an excellent antitumor effect.
A mixture of a T98G cell suspension (human glioblastoma, 1×107 cells) and Matrigel (Becton, Dickinson and Company) in equal amounts was subcutaneously transplanted to the right flank of Balb/c Slc-nu/nu (SLC Japan, Inc., male, 6 weeks old). After 77 days, the mice were grouped when their tumors became 300 to 1,200 mm3. Phosphate-buffered saline (PBS) or the radiolabeled compound [90Y]-(P2) was administered to the tail veins of the mice, and their tumor volumes were measured. The evaluation values were calculated in the same way as in Test Example 8 to evaluate the antitumor effect. The results are shown below.
The compound shown in Table 8 exhibited an excellent antitumor effect.
A mixture of a T98G cell suspension (human glioblastoma, 1×107 cells) and Matrigel (Becton, Dickinson and Company) in equal amounts was subcutaneously transplanted to the right flank of Balb/cAJcl-nu/nu (CLEA Japan, Inc., female, 6 weeks old). After 90 days, the mice were grouped when their tumors BECAME 100 to 400 mm3. Phosphate-buffered saline (PBS) or the radiolabeled compound [90Y]-(A8) was administered to the tail veins of the mice, and their tumor volumes were measured. The evaluation values were calculated in the same way as in Test Example 8 to evaluate the antitumor effect. The results are shown below.
The compound shown in Table 9 exhibited an excellent antitumor effect.
Blood kinetic parameters were calculated with OLINDA/EXM 1.0 from the blood concentration of radioactivity by blood collection over time from a cynomolgus monkey using [111In]-(P2). Also, absorbed doses in each organ in the case of administration to humans were calculated with OLINDA/EXM 1.0 from organ distribution by imaging using [111In]-(P2). The radiolabeled compound [111In]-(P2) (98 MBq/9.3 g) was administered to a cynomolgus monkey (Hamri Co., Ltd., male, 3 years old, 3.4 kg) under anesthesia. After the administration, blood collection and imaging with a gamma camera were performed over time. The blood collection was carried out 10 minutes, 30 minutes, 60 minutes, 2 hours, 4 hours, 5 hours, 6 hours, 24 hours, 48 hours, 72 hours, and 144 hours after administration. The imaging was carried out 1, 2, 4, 6, 24, 48, 72, and 144 hours after administration with a gamma camera (Symbia, Siemens AG) to take planar images. The anesthesia was carried out using 20 mg/kg ketamine before administration of [111In]-(P2) and maintained by inhalation anesthesia (2 to 3% isoflurane, 5 to 8 L/min) until the completion of the imaging at 6 hours after administration. At or after 24 hours after administration, blood collection and imaging were performed by the introduction of 20 mg/kg ketamine and 2 mg/kg xylazine.
AUC was 0.22 (% ID·h/mL), T1/2α was 0.46 (h), T1/2β was 19.3 (h), Cmax was 0.018 (% ID/mL), CL was 130.2 (mL/h/kg), and Vss was 3.52 (L/kg).
The complex of the compound or the salt thereof with a metal of the present invention has high accumulation and persistence in integrin-expressing cells such as cancer cells and exhibits fast blood clearance. Therefore, the complex is useful for diagnosis or treatment, etc., of a disease involving integrin expression.
| Number | Date | Country | Kind |
|---|---|---|---|
| 2013-196712 | Sep 2013 | JP | national |
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/JP2014/075332 | Sep 2014 | US |
| Child | 15078738 | US |
