Patent Application
20220042063
The present application is being filed along with a Sequence Listing in electronic format. The Sequence Listing is provided as a file entitled 54452668_1. TXT, created and last modified on Oct. 25, 2021, which is 20.4 Kb in size. The information in the electronic format of the Sequence Listing is incorporated herein by reference in its entirety.
The present invention relates to a novel peptide for enhancing expression efficiency of a target protein, and a fusion protein comprising the same. More specifically, the present invention relates to a peptide consisting of 7 amino acids derived from urate oxidase or a peptide containing a part thereof, and to a fusion protein comprising the peptide and RNA interacting domain (RID) as a fusion partner thereof.
The key to modem biotechnology is production of recombinant proteins; and in particular, it is important to produce large amounts of recombinant proteins. Recombinant proteins produced in large amounts are very important for production and recovery of active exogenous proteins, crystallization for functional studies, industrialization, and the like. To date, many recombinant protein production studies using E. coli have been conducted, because E. coli has advantages of easy manipulation, short growth time, safe expression, low cost, and easy scalability. However, since exogenous recombinant proteins produced in E. coli have low expression levels or lack adequate “post-translational chaperons” or “post-translational processing,” a disadvantage that the produced recombinant proteins result in formation of insoluble protein inclusion bodies has been pointed out (Francois Baneyx, Recombinant protein expression in Escherichia coli, Current Opinion in Biotechnology (1999), 10:411-421).
The problem that proteins are produced in insoluble form has been addressed by developing a method in which the protein is fused with a highly-soluble protein and the resulting fusion protein is expressed (Dominic Esposito and Deb K Chatterjee, Enhancement of soluble protein expression through the use of fusion tags, Current Opinion in Biotechnology (2006), 17:353-358). In addition, attempts have been made to increase solubility of recombinant proteins using an RNA binding domain such as lysyl tRNA synthetase (LysRS) (Choi, S. I. et al., Protein solubility and folding enhancement by interaction with RNA, PLoS ONE (2008), 3:e2677). However, LysRS is not only a large protein of more than about 100 kDa (its monomer size=about 58 kDa), but also has steric hindrance due to its structural limitation of having to form a dimer; and therefore, LysRS may prevent a recombinant protein from forming a three-dimensional structure or forming a dimer or higher-order multimer, or from being converted into monomers, so that the recombinant protein does not have a desired active form. Thus, RNA interacting domain (RID) (N-terminal appended RNA binding domain of lysyl tRNA synthetase) has been used as an RNA binding domain for replacing LysRS. However, RID itself has a great drawback of having a very low expression level.
Meanwhile, norovirus is one of the major pathogens causing acute gastroenteritis worldwide, with 200,000 children of developing countries under the age of 5 dying from norovirus every year. Norovirus is mainly transmitted through the fecal-oral route and causes nausea, vomiting, diarrhea, dehydration, and the like. Norovirus, a member of the Caliciviridae family, is a non-enveloped virus and is about 30 to 40 nm in diameter. This virus consists of single-stranded (+)RNA having a length of 7.6 kbp, and has three open reading frames (ORFs). Among these, ORF2 encodes the major structural protein VP1 that constitutes norovirus; and ORF3 encodes the protein VP2 that is not directly involved in structural formation thereof. VP1 has a total size of 59 kDa and forms a dimer. As such, 90 dimers result from self-assembly of VP1s, in which a total of 180 VP1s form one viral particle. The VP1 protein consists of two domains, S domain and P domain. The S domain acts to form a structure of the protein, and the P domain is involved in inducing an actual immune response.
To date, no cell culture method for norovirus is known and no suitable animal model has been developed. Therefore, in order to develop an effective norovirus vaccine, it is essential to develop a vaccine in the form of virus-like particles (VLPs) on which no limitation is imposed in terms of cell culture. VLPs are highly complex and sophisticated structures obtained by allowing viral structural proteins to be specifically expressed to exhibit a structure similar in appearance to the wild-type virus. Because of their similar structure to the wild-type virus, VLPs have advantages capable of inducing a high-level immune response in the body and stimulating both T-cell and B-cell immune pathways. In addition, VLPs are characterized by having no infectious capacity due to absence of genetic material in their formed structure, and thus being highly safe, and by showing excellent structural stability. However, VLPs have a disadvantage that due to their complex structure, it is very difficult to create complete VLPs.
It is known that norovirus VLPs can be produced in baculovirus-insect cells and can also be produced in yeast. However, although there have been reports that a structural protein (VP1) is expressed in soluble form in E. coli, there has been no report that virus-like particles (VLPs) are formed in E. coli. From the viewpoint that most casualties caused by norovirus occur in developing countries, development of norovirus VLPs that are derived from E. coli will provide a low-cost vaccine as compared with vaccines obtained using other expression systems, and thus will be able to make a great contribution to human society.
In the previous study, the present inventors revealed that RNA binding domain (RBD)-containing lysyl tRNA synthetase (LysRS) as a binding partner is involved in protein folding and increased solubility of various proteins. However, LysRS is not only a large protein of more than about 100 kDa (its monomer size=about 58 kDa), but also has steric hindrance due to its structural limitation of having to form a dimer; and therefore, LysRS may prevent a recombinant protein from forming a three-dimensional structure or forming a dimer or higher-order multimer, or from being converted into monomers, so that the recombinant protein does not have a desired active form. Thus, RNA interacting domain (RID) (N-terminal appended RNA binding domain of lysyl tRNA synthetase) has been used as an RNA binding domain for replacing LysRS. However, RID itself has a great drawback of having a very low expression level.
Although a protein expression level may be determined by several factors, the N-terminal sequence, precisely the mRNA sequence, of a protein is known to be important for the protein expression level.
Accordingly, while studying solubility and expression efficiency of proteins, the present inventors have identified that the protein, urate oxidase, is abnormally well expressed in E. coli, and have identified effects of the N-terminal sequence of urate oxidase on expression level of a target protein and on expression of RID, thereby completing the present invention.
The present invention intends to solve the above problems. The present inventors have identified that a peptide consisting of 7 amino acids derived from urate oxidase or a partial sequence thereof plays a crucial role in enhancing expression efficiency of a target protein, and the peptide consisting of 7 amino acids derived from urate oxidase is also applied to RNA interacting domain (RID) known as a solubility-enhancing partner, so that the target protein can have increased solubility as well as enhanced expression efficiency, thereby completing the present invention.
Accordingly, an object of the present invention is to provide a peptide containing 7 amino acids derived from urate oxidase or a partial sequence thereof; a polynucleotide encoding the peptide; an expression vector comprising the peptide; and a host cell transformed with the expression vector.
Another object of the present invention is to provide a fusion protein that comprises a peptide containing 7 amino acids derived from urate oxidase or a partial sequence thereof and RID as a fusion partner thereof; an expression vector comprising the fusion protein; and a host cell transformed with the expression vector.
Yet another object of the present invention is to provide a method for producing a soluble target protein, comprising the steps of: constructing an expression vector that contains a polynucleotide encoding a target protein and a polynucleotide which is linked to the 5′-end of the polynucleotide encoding the target protein and encodes a peptide that enhances expression efficiency of the target protein; introducing the expression vector into a host cell, to prepare a transformant; and culturing the transformant so that expression of a recombinant target protein is induced, and obtaining the recombinant target protein.
Still yet another object of the present invention is to provide a recombinant expression vector for producing a norovirus vaccine, the recombinant expression vector having norovirus VP1 protein as a target protein and being capable of not only enhancing solubility of the VP1 protein but also increasing expression efficiency of the VP1 protein, in which the VP1 protein is poorly expressed in prokaryotic cells; a host cell transformed with the vector; and a method for producing a norovirus vaccine using the vector and the host cell.
According to the first embodiment, the present invention intends to provide a peptide for enhancing expression efficiency of a target protein, the peptide comprising the amino acid sequence represented by SEQ ID NO: 1 or a partial sequence thereof.
As used herein, the term “urate oxidase” (EC 1.7.3.3 (uricase)) refers to an enzyme that acts on the purine degradation mechanism. Uricase is an enzyme that oxidizes uric acid to allantoin. In higher primates, including humans, uricase does not exist and uric acid is the end product of purine metabolism. The metabolites thereof, free acid and urate salt, are both insoluble in water, so they precipitate depending on individual differences and cause gout. In order to treat gout efficiently, a treatment, in which uricase that does not exist in humans is directly injected into a human, has been put to practical use.
As used herein, the term “target protein” refers to a protein that a person skilled in the art intends to produce in large amounts, and includes any protein that can be obtained by inserting a polynucleotide encoding the protein into a recombinant expression vector and causing the protein to be expressed in a host cell.
As used herein, the term “peptide for enhancing expression efficiency of a target protein” or “expression enhancer tag (eet)” refers to a short peptide sequence that is fused to the N-terminus of the target protein and expressed together therewith, thereby enhancing expression efficiency of the protein. Specifically, in the present invention, the peptide for enhancing expression efficiency of a target protein contains the 7-amino acid sequence represented by SEQ ID NO: 1 or a partial sequence thereof.
As used herein, the term “recombinant protein” or “fusion protein” refers to a protein obtained by linking another protein to or adding another amino acid sequence to the N-terminus or C-terminus of an original target protein sequence.
In the peptide for enhancing expression efficiency of a target protein according to the present invention, the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof may be derived from urate oxidase.
In addition, in the peptide for enhancing expression efficiency of a target protein according to the present invention, the partial sequence of SEQ ID NO: 1 may contain the amino acid sequence represented by SEQ ID NO: 2.
In the peptide for enhancing expression efficiency of a target protein according to the present invention, the peptide may bind to the N-terminus of the target protein.
In the peptide for enhancing expression efficiency of a target protein according to the present invention, the target protein may be at least one selected from the group consisting of antigens, antibodies, cell receptors, enzymes, structural proteins, serum, and cellular proteins.
In an embodiment of the present invention, the antigen may be norovirus-derived VP1 protein.
In addition, the present invention intends to provide a polynucleotide encoding the peptide for enhancing expression efficiency of a target protein.
In an embodiment of the present invention, the polynucleotide may be a polynucleotide encoding the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 2.
In a preferred embodiment of the present invention, the polynucleotide encoding SEQ ID NO: 1 may be a sequence represented by SEQ ID NO: 3.
In a preferred embodiment of the present invention, the polynucleotide encoding SEQ ID NO: 2 may be a sequence represented by SEQ ID NO: 4.
In addition, the present invention intends to provide an expression vector comprising the peptide for enhancing expression efficiency of a target protein.
The present invention intends to provide an expression vector, comprising: a polynucleotide encoding a target protein; and a polynucleotide encoding a peptide for enhancing expression efficiency of the target protein, linked to the 5′-end of the polynucleotide encoding the target protein.
As used herein, the term “expression vector” refers to a linear or circular DNA molecule that consists of a fragment encoding a polypeptide of interest, operably linked to an additional fragment provided for transcription on the expression vector. Such an additional fragment includes a promoter and a stop codon sequence. The expression vector also contains at least one replication origin, at least one selection marker, a polyadenylation signal, and the like. The expression vector is generally derived from plasmid or viral DNA or contains both elements.
As used herein, the term “operably linked” refers to fragments arranged to function so that transcription initiates from a promoter and proceeds, via a coding sequence, to a stop codon.
In the expression vector according to the present invention, the expression vector may be a plasmid, a viral vector, a phage particle, or a genomic insert. The expression vector may be transformed into a host cell, and then replicated irrespective of the genome of the host cell or integrated into the genome of the host cell.
In addition, the present invention intends to provide a host cell transformed with the expression vector containing the peptide for enhancing expression efficiency of a target protein.
As used herein, the term “transformation” or “introduction” refers to introducing DNA into a host so that DNA is replicable as an extrachromosomal element or by chromosomal integration completion. Examples of the method of performing transformation with the expression vector according to the present invention include, but are not limited to, electroporation, calcium phosphate (CaPO4) method, calcium chloride (CaCl2) method, microinjection, polyethylene glycol (PEG) method, DEAE-dextran method, cationic liposome method, or lithium acetate-DMSO method.
In the host cell according to the present invention, the host cell is preferably a host cell having high DNA introduction efficiency and high expression efficiency of introduced DNA, and any microorganism including prokaryotes and eukaryotes may be used. Preferably, the host cell may be E. coli.
In addition, the present invention intends to provide a method for producing a target protein with enhanced expression efficiency. The method for producing a target protein may comprise the steps of:
(A) constructing an expression vector that contains a recombinant target protein in which a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof is linked to the N-terminus of the target protein;
(B) introducing the expression vector into a host cell; and
(C) producing the recombinant target protein from the host cell.
According to the second embodiment, the present invention intends to provide a fusion protein for enhancing expression efficiency and solubility of a target protein, the fusion protein comprising: a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof; and RNA interacting domain (RID) as a fusion partner thereof.
As used herein, the term “RID”, “RNA interacting domain”, “N terminal appended RNA binding domain of lysyl tRNA synthetase”, or “LysRS RNA interacting domain” refers to a unique N-terminal extension site of LysRS involved in interaction between RNA and other proteins.
In the fusion protein for enhancing expression efficiency and solubility of a target protein according to the present invention, the amino acid sequence of SEQ ID NO: 1 may be derived from urate oxidase.
In the fusion protein for enhancing expression efficiency and solubility of a target protein according to the present invention, the peptide may bind to the N-terminus of the target protein.
In the fusion protein for enhancing expression efficiency and solubility of a target protein according to the present invention, RID may contain the amino acid sequence represented by SEQ ID NO: 7.
The fusion protein of the present invention may contain the amino acid sequence represented by SEQ ID NO: 9.
In the fusion protein for enhancing expression efficiency and solubility of a target protein according to the present invention, the target protein may be at least one selected from the group consisting of antigens, antibodies, cell receptors, enzymes, structural proteins, serum, and cellular proteins.
In addition, the present invention intends to provide an expression vector for enhancing expression efficiency and solubility of a target protein, the expression vector having a fusion protein that contains a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof, the peptide binding to the N-terminus of the target protein, and RID as a fusion partner thereof.
The expression vector of the present invention contains a polynucleotide encoding a target protein and a polynucleotide that is linked to the 5′-end of the polynucleotide encoding the target protein and encodes a fusion protein, in which the fusion protein may be a fusion protein of a peptide for enhancing expression efficiency of the target protein with RID.
In the expression vector according to the present invention, the expression vector may be a plasmid, a viral vector, a phage particle, or a genomic insert. The expression vector may be transformed into a host cell, and then replicated irrespective of the genome of the host cell or integrated into the genome of the host cell.
In addition, the present invention intends to provide a host cell transformed with an expression vector for enhancing expression efficiency and solubility of a target protein, the expression vector having a fusion protein that contains a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof, the peptide binding to the N-terminus of the target protein, and RID as a fusion partner thereof.
In the host cell according to the present invention, the host cell is preferably a host cell having high DNA introduction efficiency and high expression efficiency of introduced DNA, and any microorganism including prokaryotes and eukaryotes may be used. Preferably, the host cell may be E. coli.
In addition, the present invention intends to provide a method for producing a target protein with enhanced expression efficiency and solubility. The method for producing a target protein may comprise the steps of:
(A) constructing an expression vector that contains a recombinant target protein in which a fusion protein, which contains a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof and RID as a fusion partner thereof for increasing solubility of the target protein, is linked to the N-terminus of the target protein;
(B) introducing the expression vector into a host cell; and
(C) producing the recombinant target protein from the host cell.
In addition, the present invention intends to provide a recombinant expression vector for production of norovirus vaccine, comprising a polynucleotide that encodes: norovirus-derived VP1 protein as a target protein; and a peptide for enhancing expression efficiency of the target protein, the peptide containing 7-amino acid sequence derived from urate oxidase or a partial sequence thereof.
In the recombinant expression vector for production of norovirus vaccine of the present invention, the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof in the peptide for enhancing expression efficiency of a target protein may be derived from urate oxidase.
In addition, in the peptide for enhancing expression efficiency of a target protein according to the present invention, the partial sequence of SEQ ID NO: 1 may include the amino acid sequence represented by SEQ ID NO: 2.
The recombinant expression vector for production of norovirus vaccine of the present invention may contain a polynucleotide encoding the peptide of SEQ ID NO: 1 for enhancing expression efficiency of a target protein.
In a preferred embodiment of the present invention, the polynucleotide encoding the peptide of SEQ ID NO: 1 may be a sequence represented by SEQ ID NO: 3.
In a preferred embodiment of the present invention, the polynucleotide encoding the peptide of SEQ ID NO: 2 may be a sequence represented by SEQ ID NO: 4.
In a preferred embodiment of the present invention, the recombinant expression vector for production of norovirus vaccine may further contain: a polynucleotide encoding 1 to 6 histidines; and a polynucleotide encoding a protease recognition site.
In the recombinant expression vector for production of norovirus vaccine of the present invention, the polynucleotide encoding 1 to 6 histidines may be represented by SEQ ID NO: 10.
In the recombinant expression vector for production of norovirus vaccine of the present invention, the protease may be TEV.
In the recombinant expression vector for production of norovirus vaccine of the present invention, the polynucleotide encoding a protease recognition site may be represented by SEQ ID NO: 11.
In the expression vector according to the present invention, the expression vector may be a plasmid, a viral vector, a phage particle, or a genomic insert. The expression vector may be transformed into a host cell, and then replicated irrespective of the genome of the host cell or integrated into the genome of the host cell.
In addition, the present invention intends to provide a host cell transformed with the expression vector that contains the norovirus-derived VP1 protein; and a peptide for enhancing expression efficiency of a target protein.
In the host cell according to the present invention, the host cell is preferably a host cell having high DNA introduction efficiency and high expression efficiency of introduced DNA, and any microorganism including prokaryotes and eukaryotes may be used. Preferably, the host cell may be E. coli.
In addition, the present invention intends to provide a method for producing a norovirus vaccine with enhanced expression efficiency. The method for producing a norovirus vaccine may comprise the steps of:
(a) producing a recombinant expression vector for production of norovirus vaccine, the recombinant expression vector containing a polynucleotide that encodes: norovirus-derived VP1 protein as a target protein; and a peptide for enhancing expression efficiency of the target protein, the peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof;
(b) introducing the expression vector into a host cell, to produce a transformant; and
(c) culturing the transformant so that expression of a recombinant fusion protein is induced, and obtaining the recombinant fusion protein.
In addition, the present invention intends to provide a recombinant expression vector for production of norovirus vaccine, comprising a polynucleotide that encodes: norovirus-derived VP1 protein as a target protein; and a fusion protein for enhancing expression efficiency and solubility of the target protein, the fusion protein containing a peptide, which contains the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof, and RID as a fusion partner of the peptide.
That is, the expression vector of the present invention contains a polynucleotide encoding a target protein and a polynucleotide that is linked to the 5′-end of the polynucleotide encoding the target protein and encodes a fusion protein, in which the fusion protein may be a fusion protein of a peptide for enhancing expression efficiency of the target protein with RID.
As used herein, the term “fusion protein” or “recombinant protein” refers to a protein obtained by linking another protein to or adding another amino acid sequence to the N-terminus or C-terminus of an original target protein sequence.
In addition, the present invention intends to provide a method for producing a norovirus vaccine with enhanced expression efficiency and solubility. The method for producing a norovirus vaccine may comprise the steps of:
(A) constructing an expression vector that contains a recombinant target protein in which a fusion protein, which contains a peptide containing the amino acid sequence of SEQ ID NO: 1 or a partial sequence thereof and RID as a fusion partner thereof for increasing solubility of the target protein, is linked to the N-terminus of norovirus-derived VP1 protein;
(B) introducing the expression vector into a host cell; and
(C) producing a recombinant norovirus VP1 protein from the host cell.
The peptide containing 7-amino acid sequence derived from urate oxidase according to the present invention can improve expression efficiency of a target protein, and furthermore, the fusion protein obtained by binding the peptide to RID, which is well known as a solubility-enhancing protein, improves solubility as well as expression efficiency of the target protein, so that these can be usefully used for production of a recombinant target protein.
The peptide for enhancing expression efficiency of a target protein according to the present invention can improve expression efficiency of the target protein, and can also be applied with RID known as a solubility-enhancing fusion partner thereof, so that expression level and solubility of the target protein are simultaneously improved. Thus, such a peptide can be usefully used for production of a recombinant target protein.
Hereinafter, various examples are presented to help understand the present invention. The following examples are provided only for easier understanding of the present invention, and the scope of protection of the present invention is not limited to the following examples.
<Experimental Methods>
1. Construction of Protein Expression Vector
pGE-LysRS expression vector was used as a protein expression vector (Choi, S. I. et al., Protein solubility and folding enhancement by interaction with RNA, PLoS ONE (2008), 3:e2677). In pGE-LysRS whose expression is under control of T7 promoter, the LysRS gene is cleaved using Ndel and one of the cleavage sites present in MCS (Kpn1-BamH1-EcoRV-Sal1-Hind3), and EGFP or hRID, or other proteins were inserted into the same location. Here, the amino acid sequence (eet1) of SEQ ID NO: 1 or a part thereof (eet2, SEQ ID NO: 2) was inserted at the N-terminal position of the inserted protein. Then, target proteins to be expressed were inserted using two restriction enzyme sites in the prepared hRID or MCS of hRID vector containing the amino acid sequence of SEQ ID NO: 1.
2. Protein Expression and SDS-PAGE
The prepared protein expression vector was transformed into BL21 (DE3), BL21 (DE3)-pLysS, or BL21 (DE3)-pLysE competent cells, and culture was performed. All transformed E. coli cells were cultured in LB medium containing 50 μg/ml of ampicillin. The E. coli cells transformed with BL21*(DE3)-pLysS or BL21*(DE3)-pLysE were cultured in the medium supplemented with 34 μg/ml of chloramphenicol. Different culture temperature was used for each protein, and culture was performed at a condition of 33° C. to 37° C. When the OD600 value of E. coli reached 0.5 or higher, IPTG was added at a level of 0 μM to 1 mM to activate T7 promoter, and culture was performed at 33° C. or 37° C. for about 3 hours after addition of IPTG so that a sufficient amount of protein can be produced. Sufficiently cultured E. coli cells were centrifuged and the supernatant was removed. Then, the resulting E. coli harvest was stored. Next, 0.3 ml of PBS was added to the E. coli harvest corresponding to 5 ml of the LB medium, and ultrasonic pulverization was performed to make a lysate. Alternatively, the E. coli harvest corresponding to 1 ml was subjected to treatment with 60 μl of B-PER (Thermo Fisher Scientific), together with DNase and lysozyme at appropriate concentrations, thereby obtaining a lysate. Then, the lysate was analyzed with SDS-PAGE.
3. Fluorescence Measurement of EGFP Protein
Fluorescence was measured at Ex 485 nm/Em 520 nm using Fluostar Optima (BMG Labtech) to identify activity of the protein expressed in the sample to be analyzed.
Effect of Peptide of Present Invention on Expression Efficiency and Activity of EGFP Protein
1-1. SDS-PAGE Analysis
In pGE LysRS plasmid, LysRS was removed using Ndel and Hind3 restriction enzymes, and each of two sequences, EGFP gene sequence and a fusion form (eet1-EGFP) obtained by adding, to the N-terminus of the EGFP gene sequence, gene sequence (SEQ ID NO: 3) encoding the amino acid sequence (eet1) of SEQ ID NO: 1, was inserted into the same location. The vector is in the form of being expressed by T7 promoter and being regulated by lac operator, in which promoter activation is regulated by IPTG. The two recombinant plasmids were respectively transformed into BL21*(DE3)-pLysE competent cells, and protein expression was induced at a condition of 37° C. for 3 hours. Here, treatment with IPTG was performed at four concentrations of 0, 20, 40, and 80 μM.
As a result, as illustrated in
1-2. Protein Activity Assay
In order to check whether the expressed EGFP protein is also functional, for the EGFP sample in lysate form, which had been expressed under each condition, its fluorescence value was measured at 485 nm/520 nm using Fluostar Optima.
As a result, as illustrated in
Effect of Partial Sequence of Peptide of Present Invention
In order to identify an effect exhibited in a case where a partial sequence (eet2, SEQ ID NO: 2) of the peptide (eet1) identified in Example 1 is fused to a target protein, an EGFP fusion protein was expressed in the same manner as in Example 1. As a control, gene (SEQ ID NO: 6) encoding the lysyl tRNA synthetase-derived amino acid sequence (MSEQHAQ) represented by SEQ ID NO: 5 was used. Here, treatment with IPTG was performed at concentrations of 10, 20, 40, and 80 μM.
As a result, as illustrated in
In addition, when this was calculated in terms of activity per unit protein, it was identified that in a case of being fused with the eet1 sequence, a high-quality protein can be expressed in an amount equal to or greater than two times the control and that in a case of being fused with the eet2 sequence, a high-quality protein can be expressed in an amount equal to or greater than three times the control (
Effect of Peptide According to Present Invention on Expression Efficiency of TruB and TruB-EGFP Protein
In order to examine an effect of the peptide according to the present invention on expression of TruB protein, which is known to be poorly expressed in E. coli, the gene sequence encoding TruB protein and the gene sequence encoding TruB-EGFP protein, and their fusion forms, to each of which the nucleotide sequence of SEQ ID NO: 2 had been added, were respectively inserted into pGE LysRS plasmid using the same method as in Example 1. A total of four recombinant plasmids were transformed into BL21*(DE3)-pLysS competent cells, and protein expression was induced at a condition of 37° C. Here, treatment with IPTG was performed at 1 mM concentration, and over-expression was performed for 3 hours.
As a result, the controls (TruB and TruB-EGFP) were barely expressed to the extent that they are obscured by miscellaneous bands; however, in a case where eet1 of SEQ ID NO: 1 is fused to each of the TruB and TruB-EGFP proteins, it was identified that the respective proteins are expressed. Here, it was found that the expression level of the respective proteins is equal to or greater than three times the controls (
Effect of Peptide According to Present Invention on Expression Efficiency of RID Protein
In order to examine an effect of the peptide according to the present invention on expression of hRID protein (SEQ ID NO: 7), which had been developed as solubility-enhancing fusion partner, in E. coli, its expression level was identified using the same method as in Example 1. Specifically, the gene (SEQ ID NO: 8) encoding hRID protein, and the fusion form (SEQ ID NO: 10), in which an eet1-encoding gene had been linked to the N-terminus of hRID, were respectively inserted into pGE LysRS plasmid. The two recombinant plasmids were transformed into BL21*(DE3)-pLysS competent cells, and protein expression was induced at a condition of 37° C. Here, treatment with IPTG was performed at concentrations of 0, 20, 100, and 1000 μM, and over-expression was performed for 3 hours.
As a result, as illustrated in
Effect of Fusion Protein Containing Peptide and RID According to Present Invention on Expression Efficiency of Three Proteins, CsTA1953, CsTA37, and CsTA422
hRID was used as a fusion partner for increasing expression level and solubility of CsTA1953, CsTA37, and CsTA422 proteins, which are known to be poorly expressed in E. coli or not to be well expressed in a soluble form even in a case of being expressed. In pGE LysRS plasmid, LysRS was cleaved using Ndel and Kpn1, and the gene sequence (SEQ ID NO: 8) encoding hRID or the gene sequence (SEQ ID NO: 10) encoding the eet1-hRID fusion protein was respectively inserted at the same location. Next, CsTA1953 gene, CsTA37 gene, and CsTA422 gene were respectively inserted into the pGE-hRID plasmid and the pGE-eet1-hRID plasmid using BamH1 and Hind3.
A total of six recombinant proteins thus produced were expressed under the same IPTG concentration condition as in Example 4. Among these, for the four recombinant proteins containing CsTA37 and CsTA422, experiments were conducted only at a condition in which treatment with IPTG at 1 mM is performed. These proteins were expressed at a condition of 33° C.
As a result, as illustrated in
Effect of Fusion Protein Containing Peptide and RID According to Present Invention on Expression Efficiency of Norovirus VP1 Protein
6-1. Construction of Recombinant Expression Vector and Expression of VP1 Protein
Norovirus Hu/GII.4/Hiroshima/55/2005/JPN (NCBI access number: AB504310.1)-derived VP1 gene was used for production of norovirus VLP through E. coli, and the VP1 gene in question was obtained through gene synthesis. pGE-LysRS vector was used as an expression vector. This vector is an expression vector made by modifying pGEMEX-1 (Promega) vector. Specifically, the expression vector was cleaved by treatment with Nde I and BamHI restriction enzymes, and a DNA fragment was inserted into the cleaved expression vector, the DNA fragment consisting of the following sequences in a continuous manner: a polynucleotide sequence (SEQ ID NO: 3) encoding MSAVKAA (eet1, SEQ ID NO: 1) or a polynucleotide sequence (SEQ ID NO: 4) encoding MSAV (eet 2, SEQ ID NO: 2); a polynucleotide sequence (SEQ ID NO: 11) encoding 6-histidine tag (Histag); a polynucleotide sequence (SEQ ID NO: 12) encoding TEV recognition sequence (ENLYFQ, SEQ ID NO: 16) from which G has been removed; and a polynucleotide sequence (SEQ ID NO: 14) encoding VP1 (SEQ ID NO: 13). The thus completed recombinant plasmid was transformed into the E. coli host HMS174 (DE3). Initial culture for protein expression was performed as follows: Culture was performed at 37° C. for one day in 15 μg/ml of LB medium supplemented with 50 μg/ml of ampicillin, and then 1 ml of E. coli, which had been cultured on the previous day in 15 ml of LB medium supplemented with the same concentration of ampicillin, was added thereto. Culture was performed at 37° C. until the OD600 nm reached 0.5 to 0.7. When the appropriate OD value was achieved, overexpression was induced with 1 mM IPTG. After IPTG addition, expression was induced at two different temperatures (37° C., 16° C.). As a comparative example, norovirus VP1 conjugated with RID containing four amino acids, which had been applied in the previous study, was also expressed under the same condition. The expressed protein was collected and checked for solubility through SDS-PAGE.
As a result, as illustrated in
6-2. Identification of Effect of Partial Sequence of Peptide of Present Invention
In order to identify an effect exhibited in a case where norovirus VP1 is fused with a partial sequence (eet2, SEQ ID NO: 2) of the peptide (eet1, SEQ ID NO: 1) identified in Example 6-1, the VP1 protein was expressed in the same manner as in Example 6-1. The proteins, which were expressed at 37° C. for 3 hours after addition of 1 mM IPTG, were collected and checked for solubility through SDS-PAGE.
As a result, as illustrated in
6-3. Purification of Norovirus VP1
The proteins, for which solubility had been identified, were purified through nickel (Ni) affinity chromatography. Purification was conducted after E. coli was harvested in an amount of 500 ml, which had been ultimately obtained via 3 ml and 50 ml, using the same culture method as described above. Specifically, equilibrium was first made with A buffer [50 mM Tris-HCl (pH 7.5), 300 mM sodium chloride, 5% glycerol, 0.1 mM 2-mercaptoethanol, and 10 mM imidazole], and equilibrated Ni-NTA column (GE Healthcare Life Sciences, Little Chalfont, Buckinghamshire, UK) was used to purify sample proteins. Following the A buffer, B buffer [50 mM Tris-HCl (pH 7.5), 300 mM sodium chloride, 5% glycerol, 0.1 mM 2-mercaptoethanol, and 300 mM imidazole] was used to elute the proteins with linear gradient imidazole in a range of 10 to 300 mM. Target protein-containing fractions were identified through SDS-PAGE. Then, the fractions in question were collected and dialyzed using C buffer (store buffer) [50 mM Tris-HCl (pH 8.5), 10 mM NaCl, 0.1% 2-mercaptoethanol]. The concentration of finally-purified proteins was quantified using BSA (Amresco, Solon, Ohio, USA). The purified VP1 protein was mixed with 20% glycerol at a 1:1 ratio, and then stored at −20° C.
The results obtained by identifying the purified MSAVKAA (SEQ ID NO: 1)-RID-VP1 fusion protein with nickel affinity chromatography and the results obtained by identifying purification through SDS-PAGE are illustrated in
6-4 Amino Acid Sequence Analysis of Purified VP1
In order to check whether the protein obtained through the expression and purification experiments consists of the expected VP1 sequence, peptide mapping, and N-terminal and C-terminal sequencing were conducted by making a request to ISS (www.isslab.co.kr), a specialized analysis organization. First, it was identified that as a result of LC-MS/MS analysis of the peptide fragment appearing in trypsin treatment, such a peptide fragment exhibits an 83.9% match, in terms of amino acid sequence, with the originally expected sequence. It was identified that as a result of comparison of the N-terminal sequence 26-mer and the C-terminal sequence 3-mer, a match with NoV VP1 is observed (
6-5. Size Exclusion Chromatography
Biochemical analysis was performed to check whether the dimers of VP1 protein, obtained by cleavage with protease after TEV cleavage, form VLP. Specifically, size exclusion chromatography was performed at 4° C. through a Superdex-200 analytical gel-filtration column.
On the previous day, the fusion protein was cleaved at 16° C. for one day using AcTEV protease. The column was subjected to equilibrium with a buffer [ammonium acetate 250 mM (pH 6.0)]. After completion of the equilibrium, the VP1 sample from which the fusion partner protein had been cleaved was loaded thereon and purification was performed. After purification, calibration was performed using ferritin (440 kDa), aldolase (158 kDa), conalbumin (75 kDa), ovalbumin (44 kDa), and Blue Dextran 2000 (GE Healthcare), thereby determining the molecular weight of a protein which had appeared as a peak on the chromatogram.
From the viewpoints that the norovirus VLP showed a molecular weight of 10 MDa according to the results of the previous study and that the maximum purification limit of the column used by the present inventors was 800 kDa, it was assumed that VLP would be purified in void in a case of being properly formed. As a result of checking the chromatogram, it was found that in the void, there is a high peak appearing as norovirus VLP (
6-6. Dynamic Light Scattering Analysis
In order to identify the overall diameter of purified virus-like particles (VLPs), analysis was performed through dynamic light scattering (DLS).
As a result, it was identified that their overall diameter is observed in a size similar to 30 to 40 nm which the wild-type norovirus shows (
6-7. Identification of VLP Formation Through Electron Microscopy
In order to check whether the VP1 proteins purified in the void have formed VLP, observations were made with electron microscopy. Purified norovirus VLPs were first placed on a copper grid for 1 minute and then stained for 15 seconds using 2% uranyl acetate. The pretreated sample was dried at room temperature for 30 minutes and then photographed using transmission electron microscopy (TEM; JEM-1011, JEOL, Japan). The above experiment was carried out at the Research Support Department of Yonsei Biomedical Research Center, Yonsei University College of Medicine.
As a result, as illustrated in
As described above, the present invention has been described by way of preferred embodiments. It will be understood by those skilled in the art that various changes and modifications can be made without departing from essential features of the present invention. Therefore, it is to be understood that the above-described examples are illustrative in all aspects and not restrictive. It should be interpreted that the scope of the present invention is shown not in the above-stated description but in the claims, and that all differences falling under the scope equivalent thereto are encompassed by the present invention.
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2017-0157348 | Nov 2017 | KR | national |
| 10-2018-0145450 | Nov 2018 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2018/014495 | 11/23/2018 | WO | 00 |
