Claims
- 1. An isolated nucleic acid molecule comprising:
(a) a nucleotide sequence that encodes SEQ ID NO:2, 4, 6, 8, 16, 18, 20, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 62, 64, 66, 68, 70, 72, or 74; (b) SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73; (c) a nucleotide sequence that comprises an at least 20 consecutive base pair portion identical in sequence to an at least 20 consecutive base pair portion of SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73; (d) a nucleotide sequence that can be amplified from a Lycopersicon esculentum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10, SEQ ID NO:21 and 24, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, SEQ ID NO:26 and 28, or SEQ ID NO:59 and 60; (e) a nucleotide sequence that can be amplified from a Beta vulgaris DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:22 and 24 or SEQ ID NO:26 and 28; (f) a nucleotide sequence that can be amplified from a Helianthus annuus DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:26 and 28; (g) a nucleotide sequence that can be amplified from a Solanum tuberosum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:21 and 24, SEQ ID NO:21 and 23, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, or SEQ ID NO:26 and 28; (h) a nucleotide sequence that can be amplified from a Brassica napus DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10 or SEQ ID NO:26 and 28; (i) a nucleotide sequence that can be amplified from an Arabidopsis thaliana DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:13 and 14, SEQ ID NO:21 and 24, or SEQ ID NO:22 and 24; (j) a nucleotide sequence that can be amplified from an Nicotiana tabacum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10, SEQ ID NO:11 and 12, SEQ ID NO:21 and 24, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, or SEQ ID NO:26 and 28; or (k) a nucleotide sequence that can be amplified from an plant DNA library using the polymerase chain reaction with a pair of primers comprising the first 20 nucleotides and the reverse complement of the last 20 nucleotides of the coding sequence (CDS) of SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73.
- 2. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that encodes SEQ ID NO:2, 4, 6, 8, 16, 18, 20, 30, 32, 34, 36, 38, 40, 42, 44, 46, 48, 50, 52, 54, 56, 58, 62, 64, 66, 68, 70, 72, or 74.
- 3. An isolated nucleic acid molecule according to claim 1, comprising SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73.
- 4. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that comprises an at least 20 consecutive base pair portion identical in sequence to an at least 20 consecutive base pair portion of SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73.
- 5. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a Lycopersicon esculentum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10, SEQ ID NO:21 and 24, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, SEQ ID NO:26 and 28, or SEQ ID NO:59 and 60.
- 6. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a Beta vulgaris DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:22 and 24 or SEQ ID NO:26 and 28.
- 7. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a Helianthus annuus DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:26 and 28.
- 8. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a Solanum tuberosum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:21 and 24, SEQ ID NO:21 and 23, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, or SEQ ID NO:26 and 28.
- 9. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a Brassica napus DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10 or SEQ ID NO:26 and 28.
- 10. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from an Arabidopsis thaliana DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:13 and 14, SEQ ID NO:21 and 24, or SEQ ID NO:22 and 24.
- 11. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from an Nicotiana tabacum DNA library using the polymerase chain reaction with the pair of primers set forth as SEQ ID NO:9 and 10, SEQ ID NO:11 and 12, SEQ ID NO:21 and 24, SEQ ID NO:22 and 24, SEQ ID NO:25 and 28, or SEQ ID NO:26 and 28.
- 12. An isolated nucleic acid molecule according to claim 1, comprising a nucleotide sequence that can be amplified from a plant DNA library using the polymerase chain reaction with a pair of primers corresponding to the first 20 nucleotides and the reverse complement of the last 20 nucleotides of the coding sequence (CDS) of SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73.
- 13. A chimeric gene comprising a promoter active in plants operatively linked to the nucleic acid molecule of claim 1.
- 14. A recombinant vector comprising the chimeric gene of claim 13.
- 15. A host cell comprising the chimeric gene of claim 13.
- 16. A plant comprising the chimeric gene of claim 13.
- 17. The plant of claim 16, which is selected from the following: rice, wheat, barley, rye, corn, potato, canola, sunflower, carrot, sweet potato, sugarbeet, bean, pea, chicory, lettuce, cabbage, cauliflower, broccoli, turnip, radish, spinach, asparagus, onion, garlic, eggplant, pepper, celery, squash, pumpkin, cucumber, apple, pear, quince, melon, plum, cherry, peach, nectarine, apricot, strawberry, grape, raspberry, blackberry, pineapple, avocado, papaya, mango, banana, soybean, tobacco, tomato, sorghum and sugarcane.
- 18. Seed from the plant of claim 16.
- 19. A method of increasing SAR gene expression in a plant, comprising expressing the chimeric gene of claim 13 in said plant.
- 20. A method of enhancing disease resistance in a plant, comprising expressing the chimeric gene of claim 13 in said plant.
- 21. A PCR primer selected from the group consisting of SEQ ID NO:9-14, 21-28, 59, and 60.
- 22. A method for isolating a NIM1 homologue involved in the signal transduction cascade leading to systemic acquired resistance in plants comprising amplifying a DNA molecule from a plant DNA library using the polymerase chain reaction with a pair of primers corresponding to the first 20 nucleotides and the reverse complement of the last 20 nucleotides of the coding sequence (CDS) of SEQ ID NO:1, 3, 5, 7, 15, 17, 19, 29, 31, 33, 35, 37, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 61, 63, 65, 67, 69, 71, or 73 or with the pair of primers set forth as SEQ ID NO:9 and 10, SEQ ID NO:11 and 12, SEQ ID NO:13 and 14, SEQ ID NO:21 and 24, SEQ ID NO:22 and 24, SEQ ID NO:21 and 23, SEQ ID NO:25 and 28, SEQ ID NO:26 and 28, or SEQ ID NO:59 and 60.
- 23. The method of claim 22, wherein said plant DNA library is a Nicotiana tabacum (tobacco), Lycopersicon esculentum (tomato), Brassica napus (oilseed rape), Arabidopsis thaliana, Beta vulgaris (sugarbeet), Helianthus annuus (sunflower), or Solanum tuberosum (potato) DNA library.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Application No. ______ [Salmeron et al., attorney docket no. CGC2095], filed Mar. 9, 1999, the disclosure of which is hereby incorporated by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60219338 |
Mar 1999 |
US |
Divisions (1)
|
Number |
Date |
Country |
Parent |
09519232 |
Mar 2000 |
US |
Child |
10328675 |
Dec 2002 |
US |