Patent Application
20120015987
The present invention provides a novel crystalline form of atazanavir sulfate, process for its preparation and to pharmaceutical composition containing it.
Atazanavir sulfate is known by the chemical name (3S,8S,9S,12S)-3,12-bis(1,1-dimethylethyl)-8-hydroxy-4,11-dioxo-9-(phenylmethyl)-6-[[4-(2-pyridinyl) phenyl]methyl]-2,5,6,10,13-pentaazatetradecanedioic acid dimethyl ester sulfate. Atazanavir sulfate is an antiviral agent and anti-HIV protease inhibitor. Atazanavir sulfate is represented by the following structure:
Atazanavir and its sulfate salt may be prepared using the procedures described in DE Patent No. 2007751, U.S. Pat. No. 3,663,607 and U.S. Pat. No. 3,836,671.
Polymorphism is defined as “the ability of a substance to exist as two or more crystalline phases that have different arrangement and/or conformations of the molecules in the crystal Lattice. Thus, in the strict sense, polymorphs are different crystalline forms of the same pure substance in which the molecules have different arrangements and/or different configurations of the molecules”. Different polymorphs may differ in their physical properties such as melting point, solubility, X-ray diffraction patterns, etc. Although those differences disappear once the compound is dissolved, they can appreciably influence pharmaceutically relevant properties of the solid form, such as handling properties, dissolution rate and stability. Such properties can significantly influence the processing, shelf life, and commercial acceptance of a polymorph. It is therefore important to investigate all solid forms of a drug, including all polymorphic forms, and to determine the stability, dissolution and flow properties of each polymorphic form. Polymorphic forms of a compound can be distinguished in the laboratory by analytical methods such as X-ray diffraction (XRD), Differential Scanning calorimetry (DSC) and Infrared spectrometry (IR).
Solvent medium and mode of crystallization play very important role in obtaining a crystalline form over the other.
Atazanavir sulfate can exist in different polymorphic form, which differ from each other in terms of stability, physical properties, spectral data and methods of preparation.
U.S. Pat. No. 6,087,383 described the preparation of atazanavir bisulfate in the form of Type-I crystals which appear to be an anhydrous/desolvated crystalline form and Type-II crystals which are a hydrated, hygroscopic crystalline form.
WO Patent Publication No. 2005/108349 disclosed two crystal forms, pattern C, form E3 of atazanavir bisulfate and also disclosed process for the preparation of atazanavir bisulfate form A (equivalent to Type 1 crystals obtained in Example 3 of U.S. Pat. No. 6,087,383). According to WO Patent Publication No. 2005/108349, pattern C crystals of atazanavir bisulfate was obtained by stirring a suspension of the form A crystals of atazanavir bisulfate in water.
We have discovered a stable novel crystalline form of atazanavir sulfate. The novel crystalline form is stable over the time and has good flow properties and so, the novel crystalline form is suitable for formulating atazanavir sulfate.
One object of the present invention is to provide a novel crystalline form of atazanavir sulfate and a process for preparing it.
According to another object of the present invention is to provide pharmaceutical compositions containing the novel crystalline form of atazanavir sulfate.
According to one aspect of the present invention, there is provided a novel crystalline form of atazanavir sulfate designated as atazanavir sulfate form H1 characterized by peaks in the powder x-ray diffraction spectrum having 2θ angle positions at about 4.1, 13.6, 18.5, 19.5, 20.1 and 24.3±0.2 degrees. The powdered x-ray diffractogram (PXRD) of atazanavir sulfate crystalline form H1 is shown in
The atazanavir sulfate crystalline form H1 may be identified and differentiated from the known polymorphs by its characteristic PXRD pattern. Thus, for example, a peak at 13.6±0.2 degrees 20 is present in the PXRD of the atazanavir sulfate crystalline form H1 of the present invention, but is absent in the PXRD of the crystalline form of atazanavir bisulfate pattern C disclosed in the WO Patent Publication No. 2005/108349.
Atazanavir sulfate crystalline form H1 of present invention is further characterized by a Differential Scanning calorimetry (DSC) thermogram as shown in
According to another aspect of the present invention, there is provided a process for preparation of atazanavir sulfate form H1, which comprises:
Preferable anti solvent is ethyl acetate.
According to another aspect of the present invention, there is provided a pharmaceutical composition comprising atazanavir sulfate crystalline form H1 and a pharmaceutically acceptable excipient.
Preferable pharmaceutical composition of atazanavir sulfate crystalline form H1 is a solid oral dosage form, comprising atazanavir sulfate crystalline form H1.
X-ray powder diffraction spectrum was measured on a bruker axs D8 advance X-ray powder diffractometer having a copper-Kα radiation. Approximately 1 gm of sample was gently flattered on a sample holder and scanned from 2 to 50 degrees two-theta, at 0.03 degrees to theta per step and a step of 0.5 seconds. The sample was simply placed on the sample holder. The sample was rotated at 30 rpm at a voltage 40 KV and current 35 mA.
DSC (Differential Scanning calorimetry) measurements were performed with a DSC Q10 (TA Instruments, Inc.). About 3 mg of the powder was placed in an open aluminum pan and it was crimped with an aluminum lid. The crimped sample was then placed in the DSC cell opposite to empty aluminum pan (as reference) and the sample was scanned at 10° C./min from 50° C. to 250° C.
The invention will now be further described by the following example, which is illustrative rather than limiting.
Atazanavir sulfate (10 gm) was dissolved in methanol (50 ml) at room temperature and stirred for 20 minutes at room temperature. To the solution was added ethyl acetate (300 ml) at room temperature and stirred for 30 minutes at room temperature. The solid obtained was collected by filtration and the solid was washed with ethyl acetate (30 ml), and then dried at 50-55 deg C for 3 hours to obtain 7 gm of atazanavir sulfate crystalline form H1.
Atazanavir sulfate (15 gm) was dissolved in methanol (75 ml) at room temperature. Ethyl acetate (570 ml) was added to the solution at room temperature and stirred for 45 minutes at room temperature. The solid obtained was collected by filtration and the solid was washed with ethyl acetate (50 ml), and then dried at 50-55 deg C for 4 hours to obtain 11 gm of atazanavir sulfate crystalline form H1.
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/IN09/00034 | 1/12/2009 | WO | 00 | 3/29/2011 |
