Patent Application
20190032030
The present disclosure relates to a novel separated polypeptide having transaminase activity, a polynucleotide encoding the polypeptide, a microorganism including the polynucleotide, and a method of deaminating an amino compound by using the polypeptide or the microorganism.
Adipic acid is a dicarboxylic acidic compound having a molecular formula of (CH2)4(COOH)2. Adipic acid has been widely used as a raw material for nylon resin, plastic plasticizers, and dyes and pharmaceuticals. In particular, as an important intermediate product for the production of a polyamide, such as nylon 6,6, adipic acid has a very high commercial value.
Adipic acid is mainly produced by a chemical method involving a two-step process using a petroleum compound as a raw material. In detail, cyclic compounds, such as phenol, cyclohexane, cyclohexene, and benzene, are used as starting materials and converted to ketone-alcohol oil (KA oil) named cyclohexanone or cyclohexanol. Then, through an oxidation process using nitric acid, adipic acid is produced. Such a chemical process is highly efficient and economical, but use of benzene as a raw material and the production of an enormous amount of nitrogen oxide as a by-product are considered to be problems. In addition to these problems, due to environmental regulations that have recently been strengthened, the need for environmentally friendly processes for the production of adipic acid has emerged. In this regard, efforts to produce adipic acid through microorganisms are about to begin. However, a biosynthesis or biodegradation pathway of 6-aminocaproic acid, which can be used as an intermediate for the production of adipic acid, is not accurately known yet. If a 6-amine group of 6-aminocaproic acid is removed and a ketone group is introduced thereto, adipate semialdehyde is produced, and through an aldehyde dehydrogenase reaction, it is expected that the synthesis of adipic acid is possible (Guerrillot L. et al., Eur J Biochem., 1977, 81(1):185-92; Vandecasteele, J. P. et al., Methods Enzymol., 1982, 89: 484-490). The enzymatic conversion reaction from 6-aminocaproic acid to adipic acid, which consists of the same number of carbons as 6-aminocaproic acid, is highly valued as a novel technology as well as for its high commercial value. However, an enzyme or a microorganism that can participate in the actual reaction is not known.
In this regard, the inventors of the present disclosure isolated a novel microorganism having deamination activity while studying the deamination of 6-aminocaproic acid, and accordingly, a novel enzyme has been discovered from the novel microorganism, thereby completing the present disclosure.
The present disclosure provides a novel separated polypeptide having transaminase activity.
The present disclosure provides a polynucleotide encoding the polypeptide.
The present disclosure provides a microorganism transformed to express the novel polypeptide.
The present disclosure provides a method of deaminating an amino compound, and a method of converting a semialdehyde compound to an amino compound by using the polypeptide having the transaminase activity.
An aspect of the disclosure provides a separated polypeptide having transaminase activity, the polypeptide having an amino acid sequence of SEQ ID NO: 7 or an amino acid sequence having at least 75% homology with SEQ ID NO: 7.
The term “polypeptide having transaminase activity” as used herein refers to a polypeptide having activity of catalyzing a reversible amino group transfer reaction between an amino acid and an α-keto acid. The transaminase may be named aminotransferase.
The term “polypeptide” as used herein refers to a polymer of amino acids. In general, a form where a few amino acids are linked together is called a peptide, and a form where many amino acids are linked together is called a protein. About 20 types of amino acids, which constitute a protein, are linked to each other via chemical bonding to form a polypeptide. The polypeptide having transaminase activity of the present disclosure may include an amino acid sequence of SEQ ID NO: 7. In addition, as an amino acid sequence having at least 75%, at least 80%, at least 90%, for example, at least 95%, and for example, at least 99% homology with SEQ ID NO: 7, the polypeptide may have any amino acid sequence without limitation, as long as an amino acid sequence of the polypeptide has activity of catalyzing an amino group transfer reaction of a transaminase. In addition, as long as an amino acid sequence of the polypeptide is biologically equivalent to the polypeptide or has equivalent activity to the polypeptide, the polypeptide may include a variant or an analogue of the amino acid sequence.
The term “homology” as used herein refers to the degree of sequence identity with respect to a given polypeptide sequence or polynucleotide sequence, wherein the degree can be represented as a percentage. In the specification, homology of a sequence identical to a given polypeptide sequence or polynucleotide sequence or homology of a sequence having similar activity to that of a given polypeptide sequence or polynucleotide sequence is represented in terms of “% homology”. For example, the homology may be determined by using standard software, e.g., BLAST 2.0, to calculate parameters, such as score, identity, and similarity. Alternatively, the homology may be identified by comparing sequences according to a hybridization method, such as southern hybridization, performed under defined stringent conditions. The defined and appropriate conditions for the hybridization method may be determined in consideration of methods well known to one of ordinary skill in the art.
The polypeptide having transaminase activity may be derived from P. stutzeri. In detail, the polypeptide may be derived from P. stutzeri CJ-MKB (KCCM11587P). In one embodiment, the inventors of the present disclosure separated or isolated P. stutzeri CJ-MKB, which is a novel strain that can fix 6-aminocaproic acid as a nitrogen source, thereby obtaining a novel transaminase.
The polypeptide having transaminase activity of the present disclosure may deaminate an amino compound. The amino compound may include at least one selected from the group consisting of N-acetylornithine, gamma-aminobutyric acid (4-aminobutyric acid), 5-aminovaleric acid, and 6-aminocaproic acid, but is not limited thereto. In detail, the amino compound may be 5-aminovaleric acid or 6-aminocaproic acid. A conventionally known N-acetylornithine transaminase is known to use N-acetylornithine and N-succinyl-L-2-amino-6-oxopimelate as a substrate. However, it is not known at all whether gamma-aminobutyric acid, 5-aminovaleric acid, or 6-aminocaproic acid is used as a substrate (Rajaram V, Ratna Prasuna P, Savithri H S, Murthy M R. Structure of biosynthetic N-acetylornithine aminotransferase from Salmonella typhimurium: studies on substrate specificity and inhibitor binding, Proteins, 2008, 70(2):429-441). However, the polypeptide of the present disclosure may use, as a substrate, gamma-aminobutyric acid, 5-aminovaleric acid, or 6-aminocaproic acid, in addition to N-acetylornithine, to thereby catalyze a deamination reaction thereof. In one embodiment, N-acetylornithine, gamma-aminobutyric acid, 5-aminovaleric acid, or 6-aminocaproic acid can be deaminated by the polypeptide of the present disclosure, and the production of glutamate can be confirmed.
Another aspect of the present disclosure provides a polynucleotide encoding the polypeptide having transaminase activity.
The term “polynucleotide” as used herein refers to a polymer of nucleotides in which nucleotide monomers are linked in a long chain via covalent bonds, and in general, refers to a deoxyribonucleic acid (DNA) strain or ribonucleic acid (RNA) strain of more than a certain length.
The polynucleotide may include a nucleotide sequence encoding a polypeptide of SEQ ID NO: 7 or a polypeptide having at least 75% homology with SEQ ID NO: 7. In detail, the polynucleotide may include a base sequence of SEQ ID NO: 4. In addition, the polynucleotide may include a sequence having at least 80%, for example, at least 90%, for example, at least 95% homology with SEQ ID NO: 4, as long as the sequence is a nucleotide sequence encoding the protein having transaminase activity according to the present disclosure. In addition, due to the degeneracy of the genetic code, the polynucleotide may include a variant of the nucleotide sequence encoding the same amino acid sequence.
Another aspect of the present disclosure provides a microorganism transformed to express the polypeptide having transaminase activity. In detail, the microorganism is transformed to express a polynucleotide encoding a polypeptide having transaminase activity, and more particularly, may be transformed by a recombinant vector to which the polynucleotide is operably linked.
The polypeptide and the polynucleotide are as described above, respectively.
The term “operably linked” as used herein means that the gene sequence is functionally linked to a promoter sequence that initiates and mediates the transcription of the polynucleotide encoding the polypeptide having transaminase activity according to the present disclosure. The linkage with the expression vector can be prepared using genetic recombination technology known in the art. For example, a site-specific DNA cleavage and linkage may be prepared using a nicking enzyme and a linking enzyme.
The term “expression vector: as used herein refers to a DNA construct including a base sequence of a polynucleotide that encodes a target protein, which is operably linked to a suitable control sequence so that the target protein can be expressed in a suitable host cell. The control sequence may include a promoter for initiating the transcription, any operator sequence for controlling the transcription, a sequence encoding a suitable ribosome binding site of mRNA, and a sequence for controlling the transcription and translation termination. The vector may be transformed into a suitable host, and then, replicated or functionalized, regardless of the host genome. In addition, the vector may be integrated into the host genome itself. The vector used in the present disclosure is not particularly limited as long as the vector is replicable in a host, and any vector known in the art may be used. Examples of the conventionally used vector are a natural or recombinant plasmid, a cosmid, a virus, and a bacteriophage, but are not limited thereto. When the expression vector including the polynucleotide encoding the polypeptide having the transaminase activity is transformed or transfected into a host cell, a desirable polypeptide having transaminase activity can be expressed in the host cell.
The term “transformation” as used herein refers to the introduction of a vector, which includes a target protein-coding polynucleotide, into a host cell to allow expression of a protein encoded by the polynucleotide in the host cell. As long as the expression is allowed in the host cell, the transformed polynucleotide may include all types in any case whether the transformed polynucleotide is inserted in the chromosomes of the host cell or the transformed polynucleotide is located outside the chromosomes of the host cell. The method of transforming the vector of the present disclosure into the cell includes any method of introducing a base into a cell, and for example, the transformation may be performed by selecting suitable standard techniques known in the art, such as electroporation, calcium phosphate co-precipitation, retroviral infection, microinjection, DEAE-dextran, a cationic liposome method, or like. However, the transformation method is not limited thereto.
The term “transformed microorganism” as used herein refers to any microorganism including both a prokaryotic microorganism and a eukaryotic microorganism, as long as a microorganism can express the polypeptide having transaminase activity. The microorganism may be a strand of a microorganism belonging to the genus Escherichia, Erwinia, Serratia, Providencia, Corynebacterium, or Brevibacterium. For example, the microorganism may belong to the genus Escherichia, and for example, may be Eschericia coli.
Another aspect of the present disclosure provides a method of deaminating an amino compound, the method including adding the polypeptide having transaminase activity or a microorganism expressing the polypeptide to a solution containing the amino compound.
Regarding the method, the polypeptide having transaminase activity is as described above.
Regarding the method, the microorganism expressing the polypeptide having transaminase activity may be transformed with a recombinant vector that includes a polynucleotide encoding the polypeptide having transaminase activity. Such a transformed microorganism is as described above. The microorganism may be added to the solution containing the amino compound in the form of a culture of the microorganism or a lysate of the microorganism.
The amino compound may include, for example, at least one selected from the group consisting of N-acetylornithine, gamma-aminobutyric acid, 5-aminovaleric acid, and 6-aminocaproic acid, but is not limited thereto. In addition, the amino compound may include, for example, gamma-aminobutyric acid, 5-aminovaleric acid, or 6-aminocaproic acid.
The solution containing the amino compound may include at least one selected from the group consisting of pyruvate, oxaloacetate, and α-ketoglutarate, in addition to pyridoxal phosphate. The pyridoxal phosphate may be required as a coenzyme in the transaminase reaction of the polypeptide of the present disclosure. In addition, the pyruvate, the oxaloacetate, and the α-ketoglutarate may be used as amine acceptors to accept an amino group that is released from the amino group in the reaction.
In addition, the method of deaminating the amino compound may further include recovering the compound from which the amino group is removed from the reaction product. The compound from which the amino group is removed may include at least one selected from the group consisting of N-acetylglutamate5-semialdehyde, succinate semialdehyde, glutarate semialdehyde, and adipate semialdehyde, but is not limited thereto. Regarding the method of recovering a compound from which the amino group is removed from a cell or a culture, depending on a culturing method, a compound from which the amino group is removed is collected or recovered from the culture using a suitable method known in the art. For example, centrifugation, filtration, anion exchange chromatography, crystallization, and HPLC may be used, but embodiments are not limited thereto.
Another aspect of the present disclosure provides a method of producing an amino compound, the method including adding a polypeptide having transaminase activity or the microorganism expressing the polypeptide to a solution containing a semialdehyde compound.
Regarding the method of producing the amino compound, the polypeptide having transaminase activity is as described above. The polypeptide having transaminase activity also has catalytic activity for a reverse reaction of the deamination of the amino compound, such that a semialdehyde compound can be converted to an amino compound. The semialdehyde compound may include at least one selected from the group consisting of N-acetylglutamate 5-semialdehyde, succinate semialdehyde, glutarate semialdehyde, and adipate semialdehyde, but is not limited thereto. Regarding the method of producing the amino compound, the solution containing the semialdehyde compound may further include glutamate or aspartate, in addition to pyridoxal phosphate.
In addition, the method of producing the amino compound may further include recovering the produced amino compound from the solution. The amino compound may include at least one selected from the group consisting of N-acetylornithine, gamma-aminobutyric acid, 5-aminovaleric acid, and 6-aminocaproic acid, but is not limited thereto. The recovering of the produced amino compound may be performed by a suitable method known in the art.
The method of deaminating the amino compound may be used for the production of adipic acid. Regarding the method of producing adipic acid, the transaminase of the present disclosure or a lysate of the microorganism including the transaminase may be added to a solution containing 6-aminocaproic acid as the amino compound, and then, adipic acid may be synthesized from adipate semialdehyde that is converted from the 6-aminocaproic acid by using a suitable method known in the art. The synthesis of adipic acid from the adipate semialdehyde may be preferably performed by an aldehyde dehydrogenase reaction.
The polypeptide having transaminase activity of the present disclosure was first discovered. In addition, the method of deaminating the amino compound by using the polypeptide can be applied to a bio-based production method of adipic acid. That is, in consideration of the production of adipic acid using a conventional chemical method, the present disclosure is meaningful in terms of providing the basis for bio-based production of adipic acid using a novel path.
In detail, it shows the resultant obtained by culturing a single colony and performing centrifugation thereon, and illustrates separation of the resultant into the bio-film and the strain by using distilled water.
[5AVA: 10 mM 5-aminovaleric acid (standard); 1: 20 mM 6-aminocaproic acid (standard); 2: P. stutzeri CJ-MKB+20 mM 6-aminocaproic acid; 3: P. stutzeri CJ-MKB+10 mM 6-aminocaproic acid, and 20 mM 5-aminovaleric acid; 4: P. stutzeri CJ-MKB+20 mM 6-aminocaproic acid, 10 mM alpha-ketoglutarate, and 0.1 mM pyridoxal-phosphate; 4-1: P. stutzeri CJ-MKB+20 mM 6-aminocaproic acid, and 10 mM alpha-ketoglutarate; 5: P. stutzeri CJ-MKB+10 mM 6-aminocaproic acid, 20 mM 5-aminovaleric acid, 10 mM alpha-ketoglutarate, and 0.1 mM pyridoxal-phosphate; 5-1: P. stutzeri CJ-MKB+10 mM 6-aminocaproic acid, 20 mM 5-aminovaleric acid, and 10 mM alpha-ketoglutarate; 6: P. stutzeri CJ-MKB+20 mM 6-aminocaproic acid, and 20 mM glutamate; E: 10 mM glutamate (standard)].
[T: cell lysate; S: soluble protein].
[1: reaction between overexpressed pETDuet1 (empty vector) and 4-aminobutyric acid; 2: reaction between overexpressed polypeptide having transaminase activity of the present disclosure and 4-aminobutyric acid; 4: reaction between overexpressed pETDuet1 and 6-aminocaproic acid; 5: reaction between overexpressed polypeptide having transaminase activity of the present disclosure and 6-aminocaproic acid; 7: reaction between overexpressed pETDuet1 and N-acetylornithine; 8: reaction between overexpressed polypeptide having transaminase activity of the present disclosure and N-acetylornithine; and 10: glutamate (standard)].
[C: reaction between overexpressed pETDuet1 and 6-aminocaproic acid; D: reaction between overexpressed polypeptide having transaminase activity of the present disclosure and 6-aminocaproic acid, and E: 10 mM glutamate (standard)].
Hereinafter, one or more embodiments will be described in more detail with reference to the following examples. However, these examples are not intended to limit the scope of the present disclosure.
1) Screening of a Microorganism using 6-aminocaproic Acid as a Nitrogen Source and Analysis of 16S rRNA
6-aminocaproic acid (6-ACA) was fixed as a nitrogen source, and a novel strain, P. stutzeri CJ-MKB, which can deaminate 6-ACA, was selected through a subculture. For the selection, a minimal medium in which a microorganism can be cultured was prepared with the composition of Table 1. Here, a nitrogen source for the strain culture was 6-ACA.
In detail, a soil sample from the Gimpo Plant of CJ Cheiljedang Corp., located in Gayang-dong, Seoul, Korea, was cultured in a medium having the composition of Table 1 at a temperature of 37° C. and at a speed of 200 rpm. The cultured candidate strains were cultured again until an optical density thereof reached 0.5 under the conditions where the same medium was used at an initial inoculation optical density (OD600) of 0.05, at a temperature of 37° C., and at a speed of 200 rpm. Then, a microorganism cultured in the medium having the composition of Table 1 was selected through a subculture five times. The selected microorganism was primarily named ‘CJ-MKB’, and the following experiment was performed to confirm that the selected microorganism was a new microorganism.
The CJ-MKB strains cultured in the liquid medium were streaked and spread over an M9 agar plate to obtain colonies. The colonies obtained therefrom were resistant to ampicillin at a concentration of 25 mg/ml. In addition, it was observed that a light-colored bio-film was formed around the colonies. Two colonies were selected and cultured again in the M9 liquid medium, and genomic DNA was extracted using a genomic DNA prep kit. To identify the obtained genome, 16S ribosomal RNA (16S rRNA) sequences were analyzed. Here, primers commonly used for analysis of the 16S rRNA sequences of the microorganism, such as 27F (AGA GTT TGA TCC TGG CTC AG; SEQ ID NO: 18) and 1492R (GGT TAC CTT GTT ACG ACT T; SEQ ID NO: 19), were used, and accordingly it was confirmed that the 16S rRNA of the CJ-MKB genome had a base sequence of SEQ ID NO: 1.
The BLAST program provided by the National Center for Biotechnology Information (NCBI) was used to search for a strain having high nucleic acid homology with SEQ ID NO: 1 (http://blast.ncbi.nlm.nih.gov/Blast.cgi° C. PROGRAM=blastn&PAGE_TYPE=Blast Search&LINK_LOC=blasthome). As a result, it was confirmed that the strain had the same sequence as 16S rRNA of each of P. stutzeri strain NBRIS11, gamma proteobacterium BP44-iso8, uncultured bacterium clone 9, Escherichia coli strain BM0446, and enterobacteriaceae bacterium BM005, respectively.
Several microorganisms of the genus Pseudomonas are known to produce exopolysaccharide for the formation of a bio-film. In addition, the microorganisms of the genus Pseudomonas were also resistant to beta-lactam antibiotics, such as penicillin. In consideration of the sequence information of 16S rRNA of the CJ-MKB strain, the resistance to ampicillin, and the bio-film as shown during the culturing, the selected strain was highly expected to be a microorganism of the genus Pseudomonas. The single colony obtained on the M9 medium agar plate containing 25 mg/ml of ampicillin was cultured in an LB broth, and then centrifuged at a speed of 13,000 rpm for 1 minute, thereby obtaining a culture of the strain (
2) Sequence Analysis of Oxidoreductase
To determine whether the selected strain was a microorganism of the genus Pseudomonas, it was analyzed whether the novel CJ-MKB strain had a base sequence of an oxidoreductase that is present in the genus Pseudomonas. Here, newly designed primers, such as NCPPB 5P (ATGAGCAAGACTAACGAATCCC; SEQ ID NO: 20) and NCPPB 3P (TCCAGAATGGCCAGCCCGCG; SEQ ID NO: 21), were used to perform the sequence analysis. As a result, it was confirmed that the selected strain had a base sequence of SEQ ID NO: 2.
As a result of analysis using the BLAST problem of NCBI with respect to the base sequence of SEQ ID NO: 2, it was confirmed that the base sequence of SEQ ID NO: 2 has the same sequence as a molybdopterin-binding sequence of an oxidoreductase of the known P. stutzeri A1501 strain. In this regard, it was confirmed that selected novel CJ-MKB strain was a microorganism of the genus Pseudomonas.
3) Sequence Analysis of Transaminase
Since the nucleotide sequence confirmed to have homology is a short sequence of 714 bases, the subgrouping of the microorganism could not be classified correctly. In this regard, additional protein nucleic acid sequences were analyzed to confirm the subgrouping. When the microorganism used 6-ACA as a nitrogen source, N-ACETYLORNITHINE transaminase or 4-aminobutyrate transaminase, among transaminases, is specifically expected to be involved in an enzyme conversion reaction. Accordingly, the base sequence of the protein having the two enzymatic activities was confirmed.
In detail, for comparative analysis of the base sequences for N-acetylornithine transaminase that are present in the genomes of P. stutzeri A1501 and the selected CJ-MKB strain, argD_F2 (5′ primer: ATTTAAGGATCCGTCCGCCCCGCACACCCCGG; SEQ ID NO: 22) and argD_R2 (3′ primer: ATTTAAGAGCTCTCAGGCCTGGGTCAGCGTC; SEQ ID NO: 23) were used to analyze the nucleic acid sequences by PCR. As a result, it was confirmed that the N-acetylornithine transaminase of P. stutzeri A1501 and the N-acetylornithine transaminase of the new microorganism had the base sequences of SEQ ID NO: 3 and SEQ ID NO: 4, respectively.
In the same manner, for comparative analysis of the base sequences for 4-aminobutyrate transaminase of P. stutzeri A1501 and the selected strain, gabT_F (5′ primer: ATTTAACATATGCAACGCCGTGTCGCCGCCGTTCC; SEQ ID NO: 24) and gabT_R (3′ primer: ATTTAAGAATTCTCAGGTCAGCTCGTCGAAACACT; SEQ ID NO: 25) were used to perform PCR. As a result, it was confirmed that P. stutzeri A1501 and the selected strain had the base sequences of SEQ ID NO: 5 and SEQ ID NO: 6, respectively.
For comparative analysis of the base sequences for N-acetylornithine transaminase of P. stutzeri A1501 and the selected strain, multiple sequence alignment was used. As a result, it was confirmed that 13 nucleic acids out of 1,221 nucleic acid sequences were different (nucleic acid homology: 98.9353%). In addition, in the same manner as the above, the results of comparative analysis of the nucleic acid sequences of 4-aminobutyrate transaminase of P. stutzeri A1501 and the selected strain confirmed that 21 nucleic acids out of 1,257 nucleic acid sequences were different (nucleic acid homology: 98.3294%).
In conclusion, it was confirmed that the selected P. stutzeri CJ-MKB strain had the highest homology with P. stutzeri A1501 among the known microorganism genomic sequences to date, and thus, it is a novel strain. Accordingly, the selected strain was deposited on Oct. 22, 2014 in the Korean Culture Center of Microorganisms, and was given accession number KCCM11587P.
P. stutzeri CJ-MKB was subjected to an evaluation of deamination reactivity of 5-aminovaleric acid and 6-aminocaproic acid. Since a transaminase reaction is a substitution reaction between an amine group and a ketone group, the substrates and the products were easily identified through a color reaction in which the reaction products that were subjected to material separation using thin layer chromatography (TLC) were able to be easily identified by color reaction of an amine group of ninhydrin. To confirm transaminase activity of P. stutzeri CJ-MKB, a whole cell reaction was performed. When P. stutzeri CJ-MKB was cultured and the optical density of the culture reached 0.7, the culture was sub-cultured on a new M9 medium. Here, 6-aminocaproic acid and 5-aminovaleric acid were fixed as a nitrogen source, and then cultured. Alpha-ketoglutarate and pyridoxal phosphate that are required for the medium were added to the culture, and to confirm the degree of deamination of 5-aminovaleric acid and 6-aminocaproic acid, TLC was performed thereon. Here, the reaction volume was 100 μl, and the production of glutamate and the degree of substrate degradation, which were dependent upon various concentrations of 5-aminovaleric acid, 6-aminocaproic acid, alpha-ketoglutarate, and pyridoxal phosphate and the presence and absence of each substrate, were confirmed on TLC. After the TLC development was completed, a material containing an amine group was developed with the 3% ninhydrin solution. According to the TLC results, it was confirmed that 5-aminovaleric acid and 6-aminocaproic acid was reduced, and glutamate was produced (see
As shown in
From this experimental result, it was confirmed that P. stutzeri CJ-MKB can use 6-aminocaproic acid as a single nitrogen source, and in the presence of alpha-ketoglutarate and pyridoxal phosphate, the deamination of 5-aminovaleric acid and 6-aminocaproic acid was accelerated (see
1) Induction of Overexpression of a Polypeptide having Transaminase Activity and being Derived from P. stutzeri CJ-MKB strains in E. coli Strains
To confirm the activity of an enzyme, which is presumed to be a transaminase and expected to be involved in the deamination reaction in the new P. stutzeri CJ-MKB strain that was selected and identified in Example 1, the following experiment was carried out. Through the sequence analysis, a base sequence of a gene (hereinafter, referred to as “argD”) encoding the transaminase of the P. stutzeri CJ-MKB was confirmed (SEQ ID NO: 4).
For expression and purification, cloning was performed by adding a His-tag at the 5-terminal of the base sequence of argD for the expression. In detail, recombinant argD derived from the P. stutzeri CJ-MKB was introduced to E. coli Rosetta by using E. coli expression vector pETDuet1 (Merch Millipore, Darmstadt, Germany) to prepare a transformed strain. Then, the prepared transformed strain was added to a 3 mL LB broth medium to which 50 mg/ml of ampicillin was added, and the strain was cultured at a temperature of 37° C. for 12 hours. The cultured strain was cultured at a temperature of 37° C. in a 50 mL LB medium containing antibiotics. When the optical intensity thereof (at a wavelength of 600 nm) reached 0.8, expression was induced, and then, the strain was further cultured at a temperature of 18° C. for 48 hours. The cultured strain was washed and disrupted using a sonicator. Then, soluble proteins overexpressed after the disruption were identified by SDS-PAGE gel results (
The transformed E. coli Rosetta was named ‘E. coli Rosetta 0004-0057’, and deposited in the Korean Culture Center of Microorganisms (KCCM) on Oct. 22, 2014 under accession number of KCCM11588P.
2) Evaluation of Deamination Reactivity of Lysate of E. coli in which Polypeptides having Transaminase Activity of the Present Disclosure are Overexpressed
The reactivity of each of the amino compounds (gamma-aminobutyric acid, 6-aminocaproic acid, and N-acetylornithine) was evaluated using the lysate of E. coli in which N-acetylornithine transaminase of Example 3-1 was overexpressed. 10 mM of each of the three amino compounds, 10 mM alpha-ketoglutarate, and 0.1 mM pyridoxal phosphate were added, and the activity of the overexpressed N-acetylornithine transaminase was evaluated. 50 μl of the lysate of E. coli, in which overexpression was induced, and substrates were added to 50 mM HEPES buffer (pH 8.0) to perform a reaction at a volume of 100 μl. After 30 minutes of the reaction ata temperature of 37° C., the degree of deamination of 6-aminocaproic acid was identified by TLC (
The overexpressed N-acetylornithine transaminase was observed to be highly reactive with N-acetylornithine which is known to be an original substrate (Lane 8 of
In addition, by increasing the concentration of 6-aminocaproic acid in the same manner as in the above-described reactivity evaluation, the amount of glutamate produced in the conversion reaction was analyzed by TLC (
In conclusion, based on the above results, it was confirmed that, when the gene for a polypeptide having transaminase activity derived from P. stutzeri CJ-MKB was transformed and overexpressed in E. coli, the polypeptide of the present disclosure had deamination activity for 6-aminocaproic acid.
The deamination activity of the polypeptide for 6-aminocaproic acid was evaluated, wherein the polypeptide was derived from P. stutzeri CJ-MKB and had the transaminase activity of Example 3. In this regard, genes for N-acetylornithine transaminases derived from microorganisms of the genus Pseudomonas, such as P. mendocina, P. putida, P. resinovorans, P. syringae, and P. thermotolerans, that are considered to be capable of the same bioconversion reaction as the above, were expressed in E. coli for the evaluation of the reactivity thereof.
1) Comparison of Homology of N-acetylornithine Transaminases Derived from Various Pseudomonas Strains
Five strains were selected from microorganisms of the genus Pseudomonas, and then, nucleotides provided by the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov) and the genome program were used to identify genes and amino acid sequences for N-acetylornithine transaminases derived from P. mendocina, P. putida, P. resinovorans, P. syringae, and P. thermotolerans. Then, the homology with the amino acid sequence of the polypeptide derived from P. stutzeri CJ-MKB and having transaminase activity was compared (Table 2). Each of the enzymes derived from the microorganisms of the genus Pseudomonas showed at least about 75% homology with each other.
P. resinovorans
P. thermotolerans
P. stutzeri CJ-MKB
P. mendocina
P. syringae
P. putida
P. resinovorans
P. thermotolerans
P. stutzeri CJ-MKB
P. mendocina
P. syringae
P. putida
2) Separation and Purification of N-acetylornithine Transaminases Derived from Five Pseudomonas Strains and Polypeptide of the Present Disclosure
The genes for N-acetylornithine transaminases of five Pseudomonas strains and the gene for the polypeptide having transaminase activity derived from P. stutzeri CJ-MKB of the present disclosure in Example 4-1 were obtained, and expressed in E. coli in the same manner as in Example 3-1. Then, proteins purified using the His-tag column were obtained. As a result of comparing the obtained proteins on SDS-PAGE gel, it was confirmed that the proteins were purified in a normal manner (
3) Evaluation of Deamination Activity of N-acetylornithine Transaminases Derived from Five Pseudomonas Strains and the Polypeptide of the Present Disclosure for 6-aminocaproic Acid
The reactivities of the proteins purified in Example 4-2 for 6-aminocaproic acid were verified (Lanes 3, 5, 7, 9, 11, and 13 of
As a result, it was confirmed that the N-acetylornithine transaminase derived from P. syringae showed 56.69% conversion of 6-aminocaproic acid, whereas the polypeptide derived from P. stutzeri CJ-MKB showed 45.52% conversion of 6-aminocaproic acid in a similar manner to the other compared strains.
The same sample was used to compare the degree of aldehyde formation by using the relative activity values using Schiff's reagent (
The aldehyde production in the reaction sample of the N-acetylornithine transaminase derived from P. thermotolerans was measured as higher than the LC-Mass results, but the overall reaction degree was confirmed in the same order. In addition, it was confirmed that the LC-Mass results and the Schiff's reagent results had a correlation.
4) Evaluation of Deamination Activities of N-acetylornithine Transaminases Derived from Genus Pseudomonas Strains and the Polypeptide of the Present Disclosure for N-acetylornithine, gamma-aminobutyric Acid, and 5-aminovaleric Acid Substrates
With respect to various substrates, the reactivities of the N-acetylornithine transaminases, which were derived from five microorganisms of the genus Pseudomonas and of which reactivities were identified in Example 4-3, and reactivities of the polypeptide of the present disclosure, were evaluated. Along with 10 mM alpha-keto glutamate and 0.1 mM pyridoxal phosphate, N-acetylornithine, gamma-aminobutyric acid, or 5-aminovaleric acid was added at a concentration of 10 mM of the total reaction solution, and N-acetylornithine transaminase was added at a concentration of 0.5 mg/ml of the total reaction solution, for a reaction at a temperature of 37° C. for 1 hour. 10 μl out of 100 μl of the reactant was diluted with 180 μl of 50 mM HEPES buffer (pH 8.0), and 10 μl of Schiffs reagent was used for a reaction for 30 minutes. Then, a 96-well plate reader was used to compare relative enzymatic activities.
The deamination reaction of N-acetylornithine, which is known as the original substrate of N-acetylornithine transaminase, showed the highest activity when derived from P. mendocina (
Regarding gamma-aminobutyric acid, the highest reactivity was shown for the N-acetylornithine transaminase derived from P. resinovorans (
Regarding 5-aminovaleric acid, the highest reactivity was shown for the polypeptide derived from P. stutzeri CJ-MKB (
According to Example 4, it was confirmed that the N-acetylornithine transaminases derived from various Pseudomonas strains and the polypeptide of the present disclosure were converted to adipate semialdehyde and the like by deamination of an amine compound, such as 6-aminocaproic acid. In Example 5, as a reverse reaction of the reaction of Example 4, for confirming the conversion reactivity from adipate semialdehyde to 6-aminocaproic acid, excess glutamate was treated in the presence of adipate semialdehyde produced by N-acetylornithine transaminase to thereby confirm whether adipate semialdehyde was produced to 6-aminocaproic acid.
First, 5 mM 6-aminocaproic acid, 5 mM alpha-ketoglutarate, and 0.1 mM pyridoxal phosphate were prepared with 50 mM HEPES buffer (pH 8.0) at a volume of 100 μl, and N-acetylornithine transaminases derived from five Pseudomonas strains and the polypeptide of the present disclosure were added thereto at a concentration of 0.5 mg/ml of the total reaction solution, thereby performing a reaction at a temperature of 37° C. for 1 hour. After the completion of the reaction, the reaction sample was treated at a temperature of 100° C. for at least 5 minutes to thereby remove the enzyme activity. The heat-treated sample was centrifuged at a speed of 14,000 rpm for 10 minutes at a temperature 4° C., and then, 10 μl of the supernatant was diluted with 180 μl of 50 mM HEPES buffer (pH 8.0) (forward reaction, alpha-ketoglutarate (α-KG) reaction),
In addition, to induce a reverse reaction of the reaction above, a sample having a volume of 200 μl was prepared and treated under the same conditions as above. After performing centrifugation thereon, 20 mM glutamate and 0.5 mg/ml of enzyme were added to 97 μl of the supernatant. Then, at a volume of 100 μl, the reaction sample was reacted at a temperature of 37° C. for 1 hour, followed by treatment at a temperature of 100° C. for 5 minutes. Afterwards, centrifugation was performed thereon at a speed of 14,000 rpm for 10 minutes at a temperature 4° C., and then, 10 μl of the supernatant was diluted with 180 μl of 50 mM HEPES buffer (pH 8.0) (reverse reaction, glutamate reaction).
The obtained sample was reacted with 10 μl of Schiff's reagent for 30 minutes, and a 96-well plate reader was used to compare the relative enzymatic activities. As a result, when comparing the values obtained using a 96-well plate reader after the reaction of the Schiff's reagent, it was confirmed that the N-acetylornithine transaminases derived from 5 Pseudomonas strains and the polypeptide of the present disclosure converted adipate semialdehyde to 6-aminocaproic acid (
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2015-0094943 | Jul 2015 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2016/005870 | 6/3/2016 | WO | 00 |
