NUCLEAR CELL CYCLE AND MITOTIC MEMBRANE FUNCTION

Information

  • Research Project
  • 6386553
  • ApplicationId
    6386553
  • Core Project Number
    R01GM054729
  • Full Project Number
    5R01GM054729-06
  • Serial Number
    54729
  • FOA Number
  • Sub Project Id
  • Project Start Date
    5/1/1997 - 27 years ago
  • Project End Date
    4/30/2002 - 22 years ago
  • Program Officer Name
    SHAPIRO, BERT I.
  • Budget Start Date
    5/1/2001 - 23 years ago
  • Budget End Date
    4/30/2002 - 22 years ago
  • Fiscal Year
    2001
  • Support Year
    6
  • Suffix
  • Award Notice Date
    4/23/2001 - 23 years ago
Organizations

NUCLEAR CELL CYCLE AND MITOTIC MEMBRANE FUNCTION

DESCRIPTION: Mutants defective in the normal cell cycle of yeast have been identified, some of which arrest with a nuclear division defect. Mutations in the CDC48 gene are conditionally defective in completing the segregation of mother and daughter nuclei, after normal migration of the nucleus into the bud-neck has occurred. Membranes isolated from mutant strains possess an in vitro defect in the fusion of nuclear membranes, as would be required for completion of nuclear fission. Biochemical evidence supports the idea that Cdc48 protein participates in membrane fusion events involving the nuclear envelope and endoplasmic reticulum. The proposed study will investigate the role of Cdc48p in nuclear envelope membrane fusion. Genetic and biochemical methodologies will be employed to investigate the mechanism of Cdc48p action during membrane fusion and to identify components that interact with Cdc48p. Subsequent analysis of Cdc48p interactors in biochemical assays will reveal if they act upstream of Cdc48p, such as cell cycle specific modulators, or if they are substrates of Cdc48p, such as proteins involved in the fusion of lipid bilayers. It is anticipated that many of these proteins will be needed for the fusion of endoplasmic reticulum membranes and thus mitosis.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    R01
  • Administering IC
    GM
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    191826
  • Sub Project Total Cost
  • ARRA Funded
  • CFDA Code
    821
  • Ed Inst. Type
  • Funding ICs
    NIGMS:191826\
  • Funding Mechanism
  • Study Section
    CTY
  • Study Section Name
    Molecular Cytology Study Section
  • Organization Name
    DIVERSA CORPORATION
  • Organization Department
  • Organization DUNS
  • Organization City
    SAN DIEGO
  • Organization State
    CA
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    921213829
  • Organization District
    UNITED STATES