Patent Application
20220409540
The present invention relates to a nucleic acid lipid particle vaccine encapsulating HPV mRNA.
Human papillomavirus (HPV) is a virus that has a circular, double-stranded DNA molecule as its genome without envelope membrane, and there are about 200 genotypes of this virus (Non-Patent Document No. 1). Among them, some genotypes transform infected cells to cancer cells. In particular, genotype 16 (HPV16) and genotype 18 (HPV18) are classified as high risk types and have been demonstrated to be associated with development of cancer represented by cervical cancer (Non-Patent Document No. 2).
The HPV genome has eight genes encoding viral proteins, which are classified into early genes (E1, E2, E4, E5 E6 and E7) and late genes (L1 and L2) according to the stage of their expression in viral life cycle. Early genes regulate viral replication and transformation of infected cells to cancer cells, while L1 and L2 are structural proteins which form a virus particle capsid (Non-Patent Document No. 3).
HPV infects keratinocyte progenitor cells which are present in the basal lamina of squamous epithelium. The HPV infection is initiated by adsorption of the capsid L1 protein to heparan sulfate proteoglycans which are present on surfaces of host cell membranes (Non-Patent Document No. 4). Since neutralizing antibodies in charge of defense against HPV infection target the L1 protein, the preventive vaccines currently available in the market contain a VLP (virus-like particle) antigen consisting of the L1 protein as a medicinal ingredient. Further, all of the three existing preventive vaccines contain L1 VLP antigens derived from HPV16 and HPV18. Although any of these vaccines has a preventive effect of 95% or more against HPV16 and HPV18 in adolescent populations naïve for HPV infection, these vaccines do not exhibit a therapeutic effect on cervical cancer or cervical dysplasia as a precancerous condition (Non-Patent Document No. 5).
Cells infected with HPV undergo abnormalities in their cell cycle due to oncoproteins E6 and E7. This occurs because the abnormalities are attributed to the inhibition of the functions of p53 and pRb involved in cell cycle or the induction of apoptotic cell death by E6 and E7 (Non-Patent Documents Nos. 6 and 7). Since the regions of E6 and E7 important for carcinogenic activity have been elucidated, it is possible to enhance the safety of vaccines by inserting mutations into these regions for inactivation when E6 and E7 are used as vaccine antigens (Non-Patent Documents Nos. 8 to 10).
Host protective immunity against HPV infection depends on induction of neutralizing antibodies as well as that of cytotoxic T cells (CTL) and helper T cells. In particular, E6 and E7, the non-structural proteins, are target antigens for CTL induction, and thus draw attention as antigens for therapeutic vaccines against cervical cancer and cervical dysplasia caused by HPV infection (Non-Patent Document No. 11).
Patent Document No. 1 discloses the nucleotide sequences of the genes for E6/E7 fusion antigens derived from HPV genotypes 6, 11, 16, 18, 31, 33, 39, 45, 52 and 58. The nucleotide sequences disclosed in this document have those for the IgE leader sequence added to the N terminus of E6 and a furin peptidase cleavage site inserted between the E6 and E7 coding regions. Further, mutations are inserted into the p53 binding region of E6 and the pRb binding region of E7 to thereby inactivate the carcinogenic activity of E6 and E7. These sequences are introduced into expression plasmids for mammals, and their medicinal efficacy as a DNA gene vaccine against HPV is evaluated in a mouse model. Immunization is conducted by intramuscular administration of the vaccine into the thigh of a mouse using electroporation.
It is an object of the present invention to provide a vaccine for preventing and/or treating infections with human papillomavirus (HPV).
The present inventors administered a lipid particle encapsulating an mRNA molecule encoding the E6 and E7 antigens of HPV to cancer cell-transplanted mice and found that a regression effect in the cancer was observed. The present invention has been achieved based on this finding.
A summary of the present invention is described as below.
(1) A lipid particle encapsulating a nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus, wherein the lipid comprises a cationic lipid represented by general formula (Ia) or a pharmaceutically acceptable salt thereof:
wherein R1 and R2 each independently represent a C1-C3 alkyl group;
L1 represents a C17-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups;
L2 represents a C10-C19 alkyl group which may have one or a plurality of C2-C4 alkanoyloxy groups or a C10-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups; and p is 3 or 4.
(2) The particle of (1) above, wherein both R1 and R2 in general formula (Ia) are a methyl group.
(3) The particle of (1) or (2) above, wherein p in general formula (Ia) is 3.
(4) The particle of any one of (1) to (3) above, wherein L1 in general formula (Ta) is a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(5) The particle of any one of (1) to (4) above, wherein L2 in general formula (Ta) is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups or a C10-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(6) The particle of any one of (1) to (4) above, wherein L2 in general formula (Ta) is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups or a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(7) The particle of any one of (1) to (6) above, wherein L1 in general formula (Ta) is an (R)-11-acetyloxy-cis-8-heptadecenyl group, a cis-8-heptadecenyl group or a (8Z,11Z)-heptadecadienyl group.
(8) The particle of any one of (1) to (7) above, wherein L2 in general formula (Ta) is a decyl group, a cis-7-decenyl group, a dodecyl group or an (R)-11-acetyloxy-cis-8-heptadecenyl group.
(9) The particle of (1), wherein the cationic lipid is represented by the following structural formula:
(10) The particle of (1), wherein the cationic lipid is represented by the following structural formula:
(11) The particle of (1), wherein the cationic lipid is represented by the following structural formula:
(12) The particle of any one of (1) to (11) above, wherein the lipid further comprises amphipathic lipids, sterols and PEG lipids.
(13) The particle of (12) above, wherein the amphipathic lipid is at least one selected from the group consisting of distearoyl phosphatidylcholine, dioleoyl phosphatidylcholine and dioleoyl phosphatidylethanolamine.
(14) The particle of (12) or (13) above, wherein the sterol is cholesterol.
(15) The particle of any one of (12) to (14) above, wherein the PEG lipid is 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol and/or N-[methoxy poly(ethyleneglycol) 2000]carbamoyl]-1,2-dimyristyloxypropyl-3-amine.
(16) The particle of any one of (12) to (15) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 15% or less of the amphipathic lipid, 20 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30.
(17) The particle of (16) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 5 to 15% of the amphipathic lipid, 35 to 50% of the sterol, 40 to 55% of the cationic lipid and 1 to 3% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 25.
(18) The particle of (17) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 10 to 15% of the amphipathic lipid, 35 to 45% of the sterol, 40 to 50% of the cationic lipid and 1 to 2% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 17.5 to 22.5.
(19) The particle of (18) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 10 to 15% of the amphipathic lipid, 35 to 45% of the sterol, 45 to 50% of the cationic lipid and 1.5 to 2% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 17.5 to 22.5.
(20) The particle of any one of (1) to (19) above, wherein the human papillomavirus is HPV16.
(21) The particle of (20) above, wherein the human papillomavirus is HPV16 and the E6 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 8.
(22) The particle of (20) or (21) above, wherein the human papillomavirus is HPV16 and the E7 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 9.
(23) The particle of any one of (20) to (22) above, wherein the human papillomavirus is HPV16 and the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV16 is an mRNA molecule comprising a cap structure (Cap), 5′ untranslated region (5′-UTR), a leader sequence, E6 coding region, a protease cleavage sequence (furin cleavage site), E7 coding region, 3′ untranslated region (3′-UTR) and a polyA tail (polyA).
(24) The particle of (23) above, wherein the sequence of the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV16 consists of a nucleotide sequence having at least 90% identity with any one of the sequences as shown in SEQ ID NOS: 2, 4 or 6.
(25) The particle of any one of (1) to (24) above, wherein the nucleic acid molecule comprises at least one modified nucleotide.
(26) The particle of (25) above, wherein the modified nucleotide comprises at least one of 5-substituted pyrimidine nucleotide and/or pseudouridine optionally substituted at position 1.
(27) The particle of (25) above, wherein the modified nucleotide comprises at least one selected from the group consisting of 5-methylcytidine, 5-methoxyuridine, 5-methyluridine, pseudouridine and 1-alkylpseudouridine.
(28) The particle of any one of (1) to (27) above, wherein the mean particle size is 30 nm to 300 nm.
(29) Use of the particle of any one of (1) to (28) above for manufacturing a composition for preventing and/or treating infections with human papillomavirus.
(30) The use of (29) above, wherein the infections are infections with HPV16.
(31) A composition comprising the particle of any one of (1) to (28) above.
(32) The composition of (31) above for allowing the expression of the E6 and E7 antigens of human papillomavirus in vivo or in vitro.
(33) The composition of (31) or (32) above for use as a pharmaceutical drug.
(34) The composition of (33) above for inducing immune response to human papillomavirus.
(35) The composition of (33) or (34) above for preventing and/or treating infections with human papillomavirus.
(36) A method of expressing the E6 and E7 antigens of human papillomavirus in vitro, comprising introducing into cells the composition of (31) or (32) above.
(37) A method of expressing the E6 and E7 antigens of human papillomavirus in vivo, comprising administering to a mammal the composition of any one of (31) to (35) above.
(38) A method of inducing immune response to human papillomavirus, comprising administering to a mammal the composition of (33) or (34) above.
(39) A method of preventing and/or treating infections with human papillomavirus, comprising administering to a mammal the composition of any one of (33) to (35) above.
In another aspect of the present invention, a summary of the present invention is described as below.
(1-1) A lipid particle encapsulating a nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus, wherein the lipid comprises a cationic lipid represented by general formula (Ia) or a pharmaceutically acceptable salt thereof:
wherein R1 and R2 each independently represent a C1-C3 alkyl group;
L1 represents a C17-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups;
L2 represents a C10-C19 alkyl group which may have one or a plurality of C2-C4 alkanoyloxy groups or a C10-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups; and
p is 3 or 4.
(1-2) The particle of (1-1) above, wherein both R1 and R2 in general formula (Ia) are a methyl group.
(1-3) The particle of (1-1) or (1-2) above, wherein p in general formula (Ia) is 3.
(1-4) The particle of any one of (1-1) to (1-3) above, wherein L1 in general formula (Ia) is a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(1-5) The particle of any one of (1-1) to (1-4) above, wherein L2 in general formula (Ia) is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups or a C10-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(1-6) The particle of any one of (1-1) to (1-4) above, wherein L2 in general formula (Ta) is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups or a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups.
(1-7) The particle of any one of (1-1) to (1-6) above, wherein L1 in general formula (Ta) is an (R)-11-acetyloxy-cis-8-heptadecenyl group, a cis-8-heptadecenyl group or a (8Z,11Z)-heptadecadienyl group.
(1-8) The particle of any one of (1-1) to (1-7) above, wherein L2 in general formula (Ta) is a decyl group, a cis-7-decenyl group, a dodecyl group or an (R)-11-acetyloxy-cis-8-heptadecenyl group.
(1-9) The particle of (1-1), wherein the cationic lipid is represented by the following structural formula:
(1-10) The particle of (1-1), wherein the cationic lipid is represented by the following structural formula:
(1-11) The particle of (1-1), wherein the cationic lipid is represented by the following structural formula:
(1-12) The particle of (1-9) or (1-10) above, wherein the lipid further comprises amphipathic lipids, sterols and PEG lipids.
(1-13) The particle of (1-11) above, wherein the lipid further comprises amphipathic lipids, sterols and PEG lipids.
(1-14) The particle of (1-12) above, wherein the amphipathic lipid is at least one selected from the group consisting of distearoyl phosphatidylcholine, dioleoyl phosphatidylcholine and dioleoyl phosphatidylethanolamine.
(1-15) The particle of (1-13) above, wherein the amphipathic lipid is at least one selected from the group consisting of distearoyl phosphatidylcholine, dioleoyl phosphatidylcholine and dioleoyl phosphatidylethanolamine.
(1-16) The particle of (1-12) or (1-14) above, wherein the sterol is cholesterol.
(1-17) The particle of (1-13) or (1-15) above, wherein the sterol is cholesterol.
(1-18) The particle of any one of (1-12), (1-14) or (1-16) above, wherein the PEG lipid is 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol and/or N-[methoxy poly(ethyleneglycol) carbamoyl]-1,2-dimyristyloxypropyl-3-amine.
(1-19) The particle of any on e of (1-13), (1-15) or (1-17) above, wherein the PEG lipid is 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol and/or N-[methoxy poly(ethyleneglycol) carbamoyl]-1,2-dimyristyloxypropyl-3-amine.
(1-20) The particle of any one of (1-12) to (1-19) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 22.5% or less of the amphipathic lipid, 15 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30.
(1-21) The particle of (1-20) above, wherein the amphipathic lipid amounts to 5 to 22.5%.
(1-22) The particle of (1-21) above, wherein the amphipathic lipid amounts to 10 to 22.5%
(1-23) The particle of any one of (1-20) to (1-22) above, wherein the PEG lipid amounts to 1 to 3%.
(1-24) The particle of (1-23) above, wherein the PEG lipid amounts to 1 to 2%.
(1-25) The particle of any one of (1-12), (1-14), (1-16) or (1-18) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 5 to 15% of the amphipathic lipid, 35 to 50% of the sterol, 40 to 55% of the cationic lipid and 1 to 3% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30.
(1-26) The particle of (1-25) above, wherein the amphipathic lipid amounts to 10 to 15%; the sterol amounts to 35 to 45%; the cationic lipid amounts to 40 to 50%; and the PEG lipid amounts to 1 to 2%.
(1-27) The particle of (1-26) above, wherein the amphipathic lipid amounts to 10 to 15%; the sterol amounts to 35 to 45%; the cationic lipid amounts to 45 to 50%; and the PEG lipid amounts to 1.5 to 2%.
(1-28) The particle of any one of (1-13), (1-15), (1-17) or (1-19) above, wherein the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 15 to 22.5% of the amphipathic lipid, 15 to 40% of the sterol, 40 to 60% of the cationic lipid and 1 to 3% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30.
(1-29) The particle of (1-28) above, wherein the cationic lipid amounts to 45 to 60% and the PEG lipid amounts to 1 to 2%.
(1-30) The particle of (1-29) above, wherein the amphipathic lipid amounts to 17.5 to 22.5%.
(1-31) The particle of any one of (1-20) to (1-30) above, wherein the ratio of the total lipid weight to the weight of nucleic acid is 15 to 25.
(1-32) The particle of (1-31) above, wherein the ratio of the total lipid weight to the weight of nucleic acid is 15 to 22.5.
(1-33) The particle of (1-32) above, wherein the ratio of the total lipid weight to the weight of nucleic acid is 17.5 to 22.5.
(1-34) The particle of any one of (1-1) to (1-33) above, wherein the human papillomavirus is HPV16.
(1-35) The particle of (1-34) above, wherein the human papillomavirus is HPV16 and the E6 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 8.
(1-36) The particle of (1-34) or (1-35) above, wherein the human papillomavirus is HPV16 and the E7 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 9.
(1-37) The particle of any one of (1-34) to (1-36) above, wherein the human papillomavirus is HPV16 and the nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus encodes an HPV16 E6/E7 fusion protein consisting of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 17.
(1-38) The particle of any one of (1-34) to (1-37) above, wherein the human papillomavirus is HPV16 and the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV16 is an mRNA molecule comprising a cap structure (Cap), 5′ untranslated region (5′-UTR), a leader sequence, E6 coding region, a protease cleavage sequence (furin cleavage site), E7 coding region, 3′ untranslated region (3′-UTR) and a polyA tail (polyA).
(1-39) The particle of (1-38) above, wherein the sequence of the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV16 consists of a nucleotide sequence having at least 90% identity with any one of the sequences as shown in SEQ ID NOS: 2, 4 or 6.
(1-40) The particle of any one of (1-1) to (1-33) above, wherein the human papillomavirus is HPV18.
(1-41) The particle of (1-40) above, wherein the human papillomavirus is HPV18 and the E6 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 14.
(1-42) The particle of (1-40) or (1-41) above, wherein the human papillomavirus is HPV18 and the E7 antigen thereof consists of an amino acid sequence having at least 95% identity with the amino acid sequence as shown in SEQ ID NO: 15.
(1-43) The particle of any one of (1-40) to (1-42) above, wherein the human papillomavirus is HPV18 and the nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus encodes an HPV18 E6/E7 fusion protein consisting of an amino acid sequence having 95% or more identity with the sequence as shown in SEQ ID NO: 18.
(1-44) The particle of any one of (1-40) to (1-43) above, wherein the human papillomavirus is HPV18 and the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV18 is an mRNA molecule comprising a cap structure (Cap), 5′ untranslated region (5′-UTR), a leader sequence, E6 coding region, a protease cleavage sequence (furin cleavage site), E7 coding region, 3′ untranslated region (3′-UTR) and a polyA tail (polyA).
(1-45) The particle of (1-44) above, wherein the sequence of the nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV18 consists of a nucleotide sequence having at least 90% identity with the sequence as shown in SEQ ID NO: 11 or 13.
(1-46) The particle of any one of (1-1) to (1-45) above, wherein the nucleic acid molecule comprises at least one modified nucleotide.
(1-47) The particle of (1-46) above, wherein the modified nucleotide comprises at least one of 5-substituted pyrimidine nucleotide and/or pseudouridine optionally substituted at position 1.
(1-48) The particle of (1-46) above, wherein the modified nucleotide comprises at least one selected from the group consisting of 5-methylcytidine, 5-methoxyuridine, 5-methyluridine, pseudouridine and 1-alkylpseudouridine.
(1-49) The particle of (1-46) above, wherein the modified nucleotide comprises at least one selected from the group consisting of 5-methylcytidine, 5-methyluridine and 1-methylpseudouridine.
(1-50) The particle of any one of (1-1) to (1-49) above, wherein the mean particle size is 30 nm to 300 nm.
(1-51) Use of the particle of any one of (1-1) to (1-50) above for manufacturing a composition for preventing and/or treating infections with human papillomavirus.
(1-52) The use of (1-51) above, wherein the infections are infections with HPV16 or HPV18.
(1-53) A composition comprising the particle of any one of (1-1) to (1-50) above.
(1-54) The composition of (1-53) above for allowing the expression of the E6 and E7 antigens of human papillomavirus in vivo or in vitro.
(1-55) The composition of (1-53) or (1-54) above for use as a pharmaceutical drug.
(1-56) The composition of (1-55) above for inducing immune response to human papillomavirus.
(1-57) The composition of (1-55) or (1-56) above for preventing and/or treating infections with human papillomavirus.
(1-58) A method of expressing the E6 and E7 antigens of human papillomavirus in vitro, comprising introducing into cells the composition of (1-53) or (1-54) above.
(1-59) A method of expressing the E6 and E7 antigens of human papillomavirus in vivo, comprising administering to a mammal the composition of any one of (1-53) to (1-57) above.
(1-60) A method of inducing immune response to human papillomavirus, comprising administering to a mammal the composition of (1-55) or (1-56) above.
(1-61) A method of preventing and/or treating infections with human papillomavirus, comprising administering to a mammal the composition of any one of (1-55) to (1-57) above.
According to the present invention, it becomes possible to prevent and/or treat infections with human papillomavirus. According to the present invention, it also becomes possible to prevent and/or treat diseases caused by infections with human papillomavirus (e.g., cervical cancer, cervical dysplasia, and the like). Further, the particle of the present invention has excellent property in terms of metabolic stability, in vitro activity, in vivo activity, rapidness in expression of drug efficacy, persistence of drug efficacy, physical stability, drug interaction, safety and so on, and is useful as a pharmaceutical drug for treating or preventing the above-mentioned diseases.
The present specification encompasses the contents disclosed in the specification and/or the drawings of Japanese Patent Application No. 2019-207001 based on which the present patent application claims priority.
Hereinbelow, embodiments of the present invention will be described in detail.
The present invention provides lipid particles encapsulating a nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus, wherein the lipid comprises a cationic lipid represented by general formula (Ia) or a pharmaceutically acceptable salt thereof:
wherein R1 and R2 each independently represent a C1-C3 alkyl group;
L1 represents a C17-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups;
L2 represents a C10-C19 alkyl group which may have one or a plurality of C2-C4 alkanoyloxy groups or a C10-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups; and
p is 3 or 4.
R1 and R2 in general formula (Ia) each independently represent a C1-C3 alkyl group. Preferably, both R1 and R2 are a methyl group.
p in general formula (Ia) is 3 or 4, preferably 3.
L1 in general formula (Ia) represents a C17-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups. Preferably, L1 is a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups. Specific examples of L1 include, but are not limited to, (R)-11-acetyloxy-cis-8-heptadecenyl group, cis-8-heptadecenyl group and (8Z,11Z)-heptadecadienyl group.
L2 in general formula (Ta) represents a C10-C19 alkyl group which may have one or a plurality of C2-C4 alkanoyloxy groups, or a C10-C19 alkenyl group which may have one or a plurality of C2-C4 alkanoyloxy groups. Preferably, L2 is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups, or a C10-C19 alkenyl group which may have one or a plurality of acetoxy groups. Alternatively, it is also preferable that L2 in general formula (Ta) is a C10-C12 alkyl group which may have one or a plurality of acetoxy groups, or a C17-C19 alkenyl group which may have one or a plurality of acetoxy groups. Specific examples of L2 include, but are not limited to, decyl group, cis-7-decenyl group, dodecyl group and (R)-11-acetyloxy-cis-8-heptadecenyl group.
With respect to cationic lipid (a component which constitutes the particle of the present invention), the following lipids may be enumerated as specific examples: (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate, 3-dimethylaminopropyl(9Z,12Z)-octacosa-19,22-dien-11-yl carbonate, and (7R,9Z)-18-({[3-(dimethylamino)propyloxy]carbonyl}oxy)octacosa-9-en-7-yl acetate, which are represented by the following structural formulas, respectively:
The cationic lipid represented by general formula (Ia) may be either a single compound or a combination of two or more compounds.
A method for preparing the cationic lipid represented by general formula (Ia) is disclosed in International Publication WO 2015/005253.
The lipid of the present invention may further comprise amphipathic lipids, sterols and PEG lipids.
The amphipathic lipid is a lipid which has affinity to both polar and non-polar solvents. Specific examples of the amphipathic lipid include, but are not limited to, distearoyl phosphatidylcholine, dioleoyl phosphatidylcholine, dioleoyl phosphatidylethanolamine and combinations thereof. As amphipathic lipid to be used in the particle of the present invention, distearoyl phosphatidylcholine and/or dioleoyl phosphatidylethanolamine is preferable. More preferable is distearoyl phosphatidylcholine.
The sterol is a sterol which has a hydroxy group. Specific examples of the sterol include, but are not limited to, cholesterol.
The PEG lipid is a lipid modified with PEG. Specific examples of the PEG lipid include, but are not limited to, 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol and/or N-[methoxy poly(ethyleneglycol) 2000]carbamoyl]-1,2-dimyristyloxypropyl-3-amine, or a combination thereof. Preferably, 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol is used.
The lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is not particularly limited. Preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 22.5% or less of the amphipathic lipid, 15 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30. More preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 5 to 22.5% of the amphipathic lipid, 15 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30. Still more preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 10 to 22.5% of the amphipathic lipid, 15 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity; and the ratio of the total lipid weight to the weight of nucleic acid is 15 to 30. In the above-described lipid composition, the PEG lipid more preferably amounts to 1 to 3%, still more preferably 1 to 2%, and especially preferably 1.5 to 2%, in terms of molar quantity. Further, in the above-described lipid composition, the ratio of the total lipid weight to the weight of nucleic acid is more preferably 15 to 25, still more preferably 15 to 22.5, and especially preferably 17.5 to 22.5.
When 3-dimethylaminopropyl(9Z,12Z)-octacosa-19,22-dien-11-yl carbonate or (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate is used as the cationic lipid, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is not particularly limited. Preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 15% or less of the amphipathic lipid, 20 to 55% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity. More preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 5 to 15% of the amphipathic lipid, 35 to 50% of the sterol, 40 to 55% of the cationic lipid and 1 to 3% of the PEG lipid in terms of molar quantity. Still more preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 10 to 15% of the amphipathic lipid, 35 to 45% of the sterol, 40 to 50% of the cationic lipid and 1 to 2% of the PEG lipid in terms of molar quantity. Further still more preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 10 to 15% of the amphipathic lipid, 35 to 45% of the sterol, 45 to 50% of the cationic lipid and 1.5 to 2% of the PEG lipid in terms of molar quantity. Especially preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 12.5% of the amphipathic lipid, 41% of the sterol, 45% of the cationic lipid and 1.5% of the PEG lipid in terms of molar quantity. In the above-described lipid composition, the ratio of the total lipid weight to the weight of nucleic acid is preferably 15 to 30, more preferably 15 to 25, still more preferably 15 to 22.5, and especially preferably 17.5 to 22.5.
When (7R,9Z)-18-({[3-(dimethylamino)propyloxy]carbonyl}oxy)octacosa-9-en-7-yl acetate is used as the cationic lipid, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is not particularly limited. Preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 12.5 to 22.5% of the amphipathic lipid, 15 to 45% of the sterol, 40 to 65% of the cationic lipid and 1 to 5% of the PEG lipid in terms of molar quantity. More preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 15 to 22.5% of the amphipathic lipid, 15 to 40% of the sterol, 40 to 60% of the cationic lipid and 1 to 3% of the PEG lipid in terms of molar quantity. Still more preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 15 to 22.5% of the amphipathic lipid, 15 to 40% of the sterol, 45 to 60% of the cationic lipid and 1 to 2% of the PEG lipid in terms of molar quantity. Further still more preferably, the lipid composition of the amphipathic lipid, the sterol, the cationic lipid and the PEG lipid is 17.5 to 22.5% of the amphipathic lipid, 15 to 40% of the sterol, 45 to 60% of the cationic lipid and 1 to 2% of the PEG lipid in terms of molar quantity. In the above-described lipid composition, the ratio of the total lipid weight to the weight of nucleic acid is preferably 15 to 30, more preferably 15 to 25, still more preferably 15 to 22.5, and especially preferably 17.5 to 22.5.
As regards specific combinations of lipids in the present invention, distearoyl phosphatidylcholine, dioleoyl phosphatidylcholine or dioleoyl phosphatidylethanolamine as the amphipathic lipid; cholesterol as the sterol; (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate, 3-dimethylaminopropyl(9Z,12Z)-octacosa-19,22-dien-11-yl carbonate, or (7R,9Z)-18-({[3-(dimethylamino)propyloxy]carbonyl}oxy)octacosa-9-en-7-yl acetate as the cationic lipid; and 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol or N-[methoxy poly(ethyleneglycol) carbamoyl]-1,2-dimyristyloxypropyl-3-amine as the PEG lipid; may be used in combination.
The following combination is preferably used: distearoyl phosphatidylcholine or dioleoyl phosphatidylethanolamine as the amphipathic lipid; cholesterol as the sterol; (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate or (7R,9Z)-18-({[3-(dimethylamino)propyloxy]carbonyl}oxy)octacosa-9-en-7-yl acetate as the cationic lipid; and 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol as the PEG lipid. More preferably, the following combination of lipids is used in the present invention: distearoyl phosphatidylcholine as the amphipathic lipid; cholesterol as the sterol; (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate or (7R,9Z)-18-({[3-(dimethylamino)propyloxy]carbonyl}oxy)octacosa-9-en-7-yl acetate as the cationic lipid; and 1,2-dimyristoyl-sn-glycerol methoxypolyethylene glycol as the PEG lipid.
The nucleic acid molecule to be encapsulated in the lipid particle in the present invention is one capable of expressing the E6 and E7 antigens of human papillomavirus. The E6 and E7 antigens of human papillomavirus to be expressed by the nucleic acid molecule encapsulated in lipid particles may be a fusion protein of the E6 and E7 antigens, and a protease cleavage sequence may be contained between the E6 and E7 antigens. The genotype of human papillomavirus is not particularly limited. HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 may be enumerated, for example. It has been demonstrated that HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59 and 68 are associated with cancer development represented by cervical cancer.
The amino acid sequence of the E6 antigen of HPV16 is shown in SEQ ID NO: 8. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes an E6 antigen of HPV16 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 8.
The amino acid sequence of the E7 antigen of HPV16 is shown in SEQ ID NO: 9. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes an E7 antigen of HPV16 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 9.
The amino acid sequence of the E6 antigen of HPV18 is shown in SEQ ID NO: 14. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes an E6 antigen of HPV18 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 14.
The amino acid sequence of the E7 antigen of HPV18 is shown in SEQ ID NO: 15. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes an E7 antigen of HPV18 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 15.
The amino acid sequence of a protease cleavage sequence (furin cleavage site) is shown in SEQ ID NO: 16. As regards protease cleavage sequence, any sequence may be used as long as it is cleaved by furin protein. Specific examples of protease cleavage sequence include, but are not limited to, a sequence represented by R-X-K/R-R (wherein R is arginine, K is lysine, and X is any amino acid) (J. Biol. Chem. 1992, 267, 16396; J. Biol. Chem. 1991, 266, 12127).
The amino acid sequence of a fusion protein of the E6/E7 antigens of HPV16 is shown in SEQ ID NO: 17. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes a fusion protein of the E6/E7 antigens of HPV16 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 17.
The amino acid sequence of a fusion protein of the E6/E7 antigens of HPV18 is shown in SEQ ID NO: 18. The nucleic acid molecule to be encapsulated in the lipid particle may be one that encodes a fusion protein of the E6/E7 antigens of HPV18 consisting of an amino acid sequence having at least 95%, preferably 96% and more preferably 97% identity with the amino acid sequence as shown in SEQ ID NO: 18.
The term “identity of amino acid sequence” refers to the rate of identical amino acid residues over full length sequence expressed in a numerical form, when the exactly matching amino acid residues at the corresponding position are taken as identical. The identity of amino acid sequence in the present invention is calculated with a sequence analysis software GENETYX-SV/RC (Genetyx Corporation). This algorism is commonly used in the art. The amino acid encoded by the nucleic acid molecule encapsulated in the particle of the present invention may have mutations (substitutions), deletions, insertions and/or additions of amino acids, as long as the encoded amino acid retains at least a certain degree of identity with SEQ ID NOS: 8, 9 and 14-18.
The amino acid encoded by the nucleic acid molecule encapsulated in the particle of the present invention retains the sequence identity as described above and may yet have substitutions, deletions, insertions and/or additions of several amino acids (preferably 10 or less, more preferably 7 or less, still more preferably 5, 4, 3, 2 or 1) per position at several positions (preferably 5 or less, more preferably 3, 2 or 1) in the amino acid sequences as shown in SEQ ID NOS: 8, 9 and 14-18.
The nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus (e.g., HPV16 or HPV18) may be an mRNA molecule comprising a cap structure (Cap), 5′ untranslated region (5′-UTR), a leader sequence, E6 coding region, a protease cleavage sequence (furin cleavage site), E7 coding region, 3′ untranslated region (3′-UTR) and a polyA tail (polyA). A cap structure (Cap) is found at the 5′ end of mRNA of many eukaryotes. This is a moiety having a 7-methylguanosine structure. Specific examples of the cap structure include, but are not limited to, cap0, cap1, cap2 and ARCA (Anti-Reverse Cap Analog), which are represented by the following structural formulas.
(wherein Base represents any nucleobase, either unmodified or modified; and RNA represents any polynucleotide.)
As a cap structure of the mRNA of the present invention, cap0 or cap1 is preferable, with cap1 being more preferable. Specific examples of the sequence of 5′ untranslated region (5′-UTR) include, but are not limited to, a sequence represented by nucleotide numbers 2 to 70 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 2 to 70 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 2 to 70 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 2 to 70 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 2 to 70 in SEQ ID NO: 13. Specific examples of the leader sequence include, but are not limited to, a sequence represented by nucleotide numbers 71 to 124 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 71 to 124 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 71 to 124 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 71 to 124 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 71 to 124 in SEQ ID NO: 13. The sequence of E6 coding region is a sequence capable of expressing the whole or part of the amino acid sequence of E6 antigen and may comprise a start codon and/or a stop codon. Specific examples of the sequence of E6 coding region include, but are not limited to, a sequence represented by nucleotide numbers 125 to 574 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 125 to 574 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 125 to 574 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 125 to 589 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 125 to 589 in SEQ ID NO: 13. Specific examples of the protease cleavage sequence (furin cleavage site) include, but are not limited to, a sequence represented by nucleotide numbers 575 to 595 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 575 to 595 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 575 to 595 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 590 to 610 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 590 to 610 in SEQ ID NO: 13. The sequence of E7 coding region is a sequence capable of expressing the whole or part of the amino acid sequence of E7 antigen and may comprise a start codon and/or a stop codon. Specific examples of the sequence of E7 coding region include, but are not limited to, a sequence represented by nucleotide numbers 596 to 889 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 596 to 889 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 596 to 889 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 611 to 925 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 611 to 925 in SEQ ID NO: 13. Specific examples of the sequence of 3′ untranslated region (3′-UTR) include, but are not limited to, a sequence represented by nucleotide numbers 890 to 1021 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 890 to 1021 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 890 to 1021 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 926 to 1057 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 926 to 1057 in SEQ ID NO: 13. Specific examples of the sequence of polyA tail (polyA) include, but are not limited to, a sequence represented by nucleotide numbers 1022 to 1123 in SEQ ID NO: 2, a sequence represented by nucleotide numbers 1022 to 1123 in SEQ ID NO: 4; and a sequence represented by nucleotide numbers 1022 to 1123 in SEQ ID NO: 6, a sequence represented by nucleotide numbers 1058 to 1159 in SEQ ID NO: 11, a sequence represented by nucleotide numbers 1058 to 1159 in SEQ ID NO: 13. Sequences of the cap structure (Cap), 5′ untranslated region (5′-UTR), leader sequence, E6 coding region, protease cleavage sequence (furin cleavage site), E7 coding region, 3′ untranslated region (3′-UTR) and polyA tail (polyA) may be modified; and the sequence of a nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV16 may consist of a nucleotide sequence having at least 90%, preferably 95% and more preferably 97% identity with any one of the sequences as shown in SEQ ID NOS: 2, 4 and 6. Further, the sequence of a nucleic acid molecule capable of expressing the E6 and E7 antigens of HPV18 may consist of a nucleotide sequence having at least 90%, preferably 95% and more preferably 97% identity with any one of the sequences as shown in SEQ ID NOS: 11 and 13.
The nucleic acid molecule to be encapsulated in the lipid particle may be in any form, as long as it is a nucleic acid molecule capable of expressing the E6 and E7 antigens of human papillomavirus. Examples that may be enumerated include single-stranded DNA, single-stranded RNA (e.g., mRNA), single-stranded polynucleotide in which DNA and RNA are mixed, double-stranded DNA, double-stranded RNA, hybrid polynucleotide of DNA-RNA, and double-stranded polynucleotide consisting of two types of polynucleotides in which DNA and RNA are mixed. Preferably, mRNA is used.
Nucleotides constituting the nucleic acid molecule to be encapsulated in the lipid particle may be either natural or modified nucleotides. Preferably, at least one of the nucleotides is a modified nucleotide.
Modified nucleotides may be modified in any moiety, i.e., base, sugar or phosphodiester bond. The modification may be at either one or two or more sites.
Examples of modified bases include, but are not limited to, cytosine as 5-methylated, 5-fluorinated or N4-methylated; uracil as 5-methylated (thymine) or 5-fluorinated; adenine as N6-methylated; and guanine as N2-methylated.
Examples of modified sugars include, but are not limited to, D-ribofuranose as 2′-O-methylated.
Examples of the modification of phosphodiester bond include, but are not limited to, phosphorothioate bond.
Preferably, modified nucleotides are those in which the base is modified. For example, 5-substituted pyrimidine nucleotide or pseudouridine optionally substituted at position 1 may be given.
Specific examples of such modified nucleotide include, but are not limited to, 5-methylcytidine, 5-methoxyuridine, 5-methyluridine, pseudouridine and 1-alkylpseudouridine. As 1-alkylpseudouridine, 1-(C1-C6 alkyl)pseudouridine may be given; and preferably, 1-methylpseudouridine or 1-ethylpseudouridine may be enumerated. More preferable examples of modified nucleotide include, but are not limited to, 5-methylcytidine, 5-methyluridine and 1-methylpseudouridine. As examples of especially preferable modified nucleotides, a combination of 5-methylcytidine and 5-methyluridine or a combination of 5-methylcytidine and 1-methylpseudouridine may be given.
The nucleic acid molecule of the present invention capable of expressing the E6 and E7 antigens of human papillomavirus (such as HPV16 or HPV18) may be prepared from a DNA having a desired nucleotide sequence by in vitro transcription reaction. Enzymes, buffers and nucleoside-5′-triphosphate mixture [adenosine-5′-triphosphate (ATP), guanosine-5′-triphosphate (GTP), cytidine-5′-tripphosphate (CTP) and uridine-5′-triphosphate (UTP)] that are necessary for in vitro transcription are commercially available (AmpliScribeT7 High Yield Transcription Kit (Epicentre), mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies), and so forth). As regards the DNA to be used for preparing a single-stranded RNA, a cloned DNA (such as plasmid DNA or DNA fragment) is used. As regards plasmid DNA or DNA fragment, commercial products may be used. Alternatively, such DNA may be prepared by methods well known in the art (for example, see those methods described in Sambrook, J. et al., Molecular Cloning a Laboratory Manual second edition (1989); Rashtchian, A., Current Opinion in Biotechnology, 1995, 6(1), 30-36; and Gibson D. G. et al., Science, 2008, 319(5867), 1215-1220).
For the purpose of obtaining an mRNA with improved stability and/or safety, it is also possible to substitute the whole or part of unmodified nucleoside-5′-triphosphate with modified nucleoside-5′-triphosphate in in vitro transcription reaction to thereby substitute the whole or part of unmodified nucleotides in mRNA with modified nucleotides (Kormann, M., Nature Biotechnology, 2011, 29, 154-157).
For the purpose of obtaining an mRNA with improved stability and/or safety, it is also possible to introduce a cap structure (Cap0 structure as defined above) at the 5′ end of mRNA after in vitro transcription reaction by a method using a capping enzyme. Further, it is possible to convert Cap0 to Cap1 by acting 2′-O-methyltransferase on mRNA having Cap0. As regards capping enzyme and 2′-O-methyltransferase, commercial products may be used (for example, Vaccinia Capping System, M2080 and mRNA Cap 2′-O-Methyltransferase, M0366, both of which are manufactured by New England Biolab). When commercial products are used, mRNA with a cap structure may be prepared according to the protocols attached to the products.
A cap structure at the 5′ end of mRNA may also be introduced by a method different from the one using enzymes. For example, it is possible to introduce into mRNA the structure of a cap analogue which ARCA has or a Cap1 structure derived from CleanCap by adding ARCA or CleanCap to in vitro transcription reaction. As regards ARCA and CleanCap, commercial products may be used (ARCA, N-7003 and CleanCap Reagent AG, N-7113, both of which are manufactured by TriLink BioTechnologies). When commercial products are used, mRNA with a cap structure may be prepared according to the protocols attached to the products.
The lipid particle encapsulating a nucleic acid molecule according to the present invention may be prepared by various methods, such as a thin film method, a reverse phase evaporation method, an ethanol injection method, an ether injection method, a dehydration-rehydration method, a detergent dialysis method, a hydration method, a freezing-thawing method, and so forth. For example, the lipid particle encapsulating a nucleic acid molecule may be prepared by the methods described in WO2015/005253. Alternatively, the lipid particle encapsulating a nucleic acid molecule according to the present invention can also be prepared by mixing a nucleic acid solution and a solution of lipids in a micro flow channel. For example, the lipid particle may be prepared with NanoAssemblr™ from Precision NanoSystems, according to the method described in the attached protocol.
The mean particle size of the particle of the present invention may be 30 nm to 300 nm, preferably 30 nm to 200 nm, and more preferably 30 nm to 100 nm. Mean particle size may be obtained by measuring volume mean particle size based on the principle of dynamic light scattering or the like using instruments such as Zeta Potential/Particle Sizer NICOMP™ 380ZLS (Particle Sizing Systems).
The particle of the present invention may be used for preparing a composition for preventing and/or treating those diseases caused by human papillomavirus infections (cervical cancer, cervical dysplasia, anal cancer, oropharyngeal cancer and condyloma acuminatum). The infection may be with a genotype of HPV16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 68, 6 or 11. Infection with HPV16 and/or HPV18 is preferable; and infection with HPV16 is more preferable.
It is possible to express the E6 and E7 antigens of human papillomavirus in vivo or in vitro using the particle of the present invention. Therefore, the present invention provides a method of expressing the E6 and E7 antigens of human papillomavirus in vitro, comprising introducing into cells a composition containing the above-described lipid particle. Further, the present invention also provides a method of expressing the E6 and E7 antigens of human papillomavirus in vivo, comprising administering to a mammal a composition containing the above-described lipid particle. By expressing the E6 and E7 antigens of human papillomavirus in vivo, it is possible to induce immune response to human papillomavirus. As a result, it becomes possible to prevent and/or treat human papillomavirus infections. Therefore, the present invention provides a method of inducing immune response to human papillomavirus, comprising administering to a mammal a composition containing the above-described lipid particle. Further, the present invention provides a method of preventing and/or treating infections with human papillomavirus, comprising administering to a mammal a composition containing the above-described lipid particle.
The particle of the present invention may be used as a pharmaceutical drug or an experimental reagent. The particle of the present invention is usually added to a carrier (such as water, buffer, saline, etc.), and the resultant formulation (composition) may be introduced into a cell (in vitro) or administered to a mammal (in vivo). When the composition is administered to a mammal, the carrier may be a pharmacologically acceptable carrier (e.g., saline). Further, the particle of the present invention may also be prepared into such formulations as cream, paste, ointment, gel, lotion or the like that comprise fat, fatty oil, lanolin, vaseline, paraffin, wax, resin, plastic, glycols, higher alcohol, glycerol, water, emulsifier, suspending agent, and the like as base materials.
The particle of the present invention may be administered to a mammal such as human, mouse, rat, hamster, guinea pig, rabbit, pig, monkey, cat, dog, goat, sheep, cattle, etc. orally or parenterally through various routes such as intramuscular, intravenous, rectal, transdermal, transmucosal, subcutaneous or intradermal administration.
When the particle of the present invention is administered to a human, the particle may be administered, for example, at an approximate dose of 0.001-1 mg, preferably 0.01-0.2 mg per adult per administration either once or several times by intramuscular injection, subcutaneous injection, intradermal injection, intravenous infusion or intravenous injection. The dose and the number of times of administration may be changed appropriately depending on the type and symptoms of the disease, the age of the patient, administration route, etc.
When the particle of the present invention is used as an experimental reagent, it is possible to express the E6 and E7 antigens of human papillomavirus in vitro by introducing the particle into a cell in which expression of the E6 and E7 antigens of human papillomavirus is desired [e.g., HEK293 cells and cells derived therefrom (HEK293T cells, FreeStyle 293 cells, Expi293 cells, etc.), CHO cells, C2Cl2 mouse myoblast cells, immortalized mouse dendritic cells (MutuDC1940), or the like]. The expression of the E6 and E7 antigens of human papillomavirus may be analyzed by detecting the E6 and E7 antigen proteins of human papillomavirus in samples based on Western blotting or by detecting peptide fragments specific to the E6 and E7 antigens of human papillomavirus based on mass spectrometry.
As used herein, the term “treat” refers to recovery, amelioration, relaxation and/or delaying the progression of clinical symptoms of diseases in patients who are developing infections with viruses or bacteria or diseases caused by such infections (e.g., precancerous lesion or cancer).
As used herein, the term “prevent” refers to reducing the incidence rate of diseases caused by infections with viruses or bacteria. “Prevent” encompasses lowering the risk of progression of diseases caused by infections with viruses or bacteria, or reducing exacerbation of such diseases. Since the particle of the present invention induces protective immune response, the particle of the present invention shows effectiveness on prevention and/or treatment of the above-described diseases.
Hereinbelow, the present invention will be described specifically with reference to the following examples. These examples are given only for explanation and are not intended to limit the scope of the present invention.
A plasmid was constructed in order to prepare a template DNA for in vitro transcription (IVT) of HPV16 E6-E7 fusion2. Briefly, a DNA fragment (SEQ ID NO: 1) containing GCTAGC (NheI site), T7 promoter sequence, 5′-UTR sequence of human P-globin, KOZAK sequence, coding region for IgE leader sequence-HPV16 E6-furin cleavage site-HPV16 E7, 3′-UTR sequence of human β-globin, polyA tail, and ACTAGT (SpeI site) was prepared by ligation in this order and then introduced into a plasmid to generate a plasmid of interest (pMA-HPV16_fusion2).
The plasmid generated in Example 1-(1) (250 μg) was dissolved in Nnuclease-Free Water (2200 μl, Thermo Fisher catalog #AM9937). To this solution, 10× CutSmart Buffer (250 μl, New England Biolabs catalog #B7204S) and SpeI-HF (30 μl, New England Biolabs catalog #R3133L) were added, and the resultant mixture was incubated at 37° C. for 2 hours and then at 65° C. for 20 minutes. 7.5 M ammonium acetate (1250 μl) and ethanol (7500 μl) were added and mixed with the incubated solution, which was then left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and the precipitate obtained was suspended in 70% ethanol. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the resultant precipitates were collected and air-dried. TE-Buffer was added to the dried precipitate to prepare a template DNA solution of 500 μg/ml.
(3) Preparation of HPV16 E6-E7 Fusion2 mRNA-001 by In Vitro Transcription
The 500 μg/ml template DNA solution from Example 1-(2) (200 μl), 100 mM CleanCap AG (200 μl, TriLink catalog #T-7113), 100 mM ATP (200 μl, Hongene catalog #R1331), 100 mM GTP (200 μl, Hongene catalog #R2331), 100 mM 5-Me-CTP (200 μl, Hongene catalog #R3-029), 100 mM 5-methyluridine triphosphate (200 μl), Nuclease-Free Water (1600 μl, Thermo Fisher catalog #AM9937), T7 Transcription 5× buffer (800 μl, Promega catalog #P140X), Enzyme mix, and T7 RNA Polymerase (400 μl, Promega catalog #P137X) were mixed, and incubated at 37° C. for 4 hours. RQ1 RNase-Free DNase (100 μl, Promega catalog #M6101) was added, and the resultant mixture was incubated at 37° C. for 15 minutes. 8 M LiCl solution (2000 μl, Sigma-Aldrich catalog #L7026) was also added, and the mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water, followed by purification using RNeasy Maxi kit (Qiagen catalog #75162) according to the attached manual. The eluate obtained (10.3 ml; corresponding to 17013 μg DNA on the basis of UV absorbance), Nuclease-Free Water (247 μl), and rApid Alkaline Phosphatase (3403 μl) and the buffer (1550 μl) for this enzyme (Roche catalog #04 898 141 001) were mixed, incubated at 37° C. for 1 hour and then at 75° C. for 15 minutes. 8M LiCl solution (7750 μl) was added, and the resultant mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water and passed through two-connected columns of reversed phase high performance liquid chromatography (Chromolith Semi-Prep (Merck catalog #1.52016.0001)) with 5% acetonitrile, 400 mM triethylamine acetate (pH 7.0)/25% acetonitrile, and 400 mM triethylamine acetate (pH 7.0, 80° C.) used as eluents to purify the mRNA of interest.
The resultant mRNA has the sequence as shown in SEQ ID NO: 2. The mRNA was analyzed with Experion RNA StdSens (BIO-RAD catalog #7007103JA) to thereby confirm that the mRNA has an anticipated nucleotide length.
A plasmid was constructed in order to prepare a template DNA for IVT of HPV16 E6-E7 fusion10. Briefly, a DNA fragment (SEQ ID NO: 3) containing GCTAGC (NheI site), T7 promoter sequence, 5′-UTR sequence of $-globin, KOZAK sequence, coding region for IgE leader sequence-HPV16 E6-furin cleavage site-HPV16 E7, 3′-UTR sequence of $-globin, polyA tail, and ACTAGT (SpeI site) was prepared by ligation in this order and then introduced into a plasmid to generate a plasmid of interest (pMA-HPV16_fusion10).
(2) Preparation of HPV16 E6-E7 Fusion10 mRNA-001 by In Vitro Transcription
Using the plasmid from Example 2-(1) instead of the plasmid from Example 1-(1), the mRNA was obtained in the same manner as described in Example 1-(2) and -(3).
The resultant mRNA has the sequence as shown in SEQ ID NO: 4. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
Using the plasmid from Example 2-(1) instead of the plasmid from Example 1-(1), a template DNA was obtained in the same manner as described in Example 1-(2). Subsequently, using the resultant template DNA and 100 mM Pseudo-UTP (Hongene catalog #R5-022) instead of the template DNA from Example 1-(3) and 100 mM 5-Me-UTP, respectively, the mRNA was obtained in the same manner as described in Example 1-(3).
The resultant mRNA has the sequence as shown in SEQ ID NO: 4. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
Using the plasmid from Example 2-(1) instead of the plasmid from Example 1-(1), a template DNA was obtained in the same manner as described in Example 1-(2). Subsequently, using the resultant template DNA, 100 mM CTP (Hongene catalog #R3331), and 100 mM N1-methylpeseudouridine-5′-triphosphate (TriLink catalog #N-1081) instead of the template DNA from Example 1-(3), 100 mM 5-Me-CTP, and 100 mM 5-Me-UTP, respectively, the mRNA was obtained in the same manner as described in Example 1-(3).
The resultant mRNA has the sequence as shown in SEQ ID NO: 4. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
Using the plasmid from Example 2-(1) instead of the plasmid from Example 1-(1), a template DNA was obtained in the same manner as described in Example 1-(2). Subsequently, using the resultant template DNA and 100 mM CTP (Hongene catalog #R3331) instead of the template DNA from Example 1-(3) and 100 mM 5-Me-CTP, respectively, the mRNA was obtained in the same manner as described in Example 1-(3).
The resultant mRNA has the sequence as shown in SEQ ID NO: 4. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
A plasmid was constructed in order to prepare a template DNA for IVT of HPV16 E6-E7 fusion10 opt2. Briefly, a DNA fragment (SEQ ID NO: 5) containing GCTAGC (NheI site), T7 promoter sequence, 5′-UTR sequence of $-globin, KOZAK sequence, coding region for IgE leader sequence-HPV16 E6-furin cleavage site-HPV16 E7, 3′-UTR sequence of $-globin, polyA tail, and ACTAGT (SpeI site) was prepared by ligation in this order and then introduced into a plasmid to generate a plasmid of interest (pMA-HPV16_fusion10_opt2).
The plasmid generated in Example 6-(1) (250 μg) was dissolved in Nnuclease-Free Water (2200 μl, Thermo Fisher catalog #AM9937). To this solution, 10× CutSmart Buffer (250 μl, New England Biolabs catalog #B7204S) and SpeI-HF (30 μl, New England Biolabs catalog #R3133L) were added, and the resultant mixture was incubated at 37° C. for 2 hours and then at 65° C. for 20 minutes. 7.5 M ammonium acetate (1250 μl) and ethanol (7500 μl) were added and mixed with the incubated solution, which was then left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the resultant precipitate was collected and air-dried. TE-Buffer was added to the dried precipitate to prepare a template DNA solution of 500 μg/ml.
(3) Preparation of HPV16 E6-E7 Fusion10 Opt2 mRNA-001 by In Vitro Transcription
500 μg/ml template DNA from Example 6-(2) (100 μl), 100 mM ATP (150 μl, Hongene catalog #R1331), 100 mM GTP (150 μl, Hongene catalog #R2331), 100 mM CTP (150 μl, Hongene catalog #R3331), 100 mM N1-methylpseudouridine-5′-triphosphate (150 μl, Hongene catalog #R5-027), Nuclease-Free Water (700 μl, Thermo Fisher catalog #AM9937), T7 Transcription 5× buffer (400 μl, Promega catalog #P140X), Enzyme mix, and T7 RNA Polymerase (200 μl, Promega catalog #P137X) were mixed, and-incubated at 37° C. for 4 hours. RQ1 RNase-Free DNase (50 μl, Promega catalog #M6101) was added, and the resultant mixture was incubated at 37° C. for 15 minutes. 8 M LiCl solution (1000 μl, Sigma-Aldrich catalog #L7026) was further added, and the mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water. To this solution (962 μl; corresponding to 5400 g of RNA on the basis of UV absorbance), Nuclease-Free Water (2818 μl) was added. The resultant solution was heated at 70° C. for 20 minutes and then cooled on ice for 10 minutes. To this solution, 540 μl of 10× capping buffer (500 mM Tris-HCl (pH 8.0), 50 mM KCl, 10 mM MgCl2, 50 mM DTT), 20 mM GTP (270 μl; 100 mM GTP, prepared by diluting Hongene catalog #R2331 with Nuclease-FreeWater), 20 mM SAM (270 μl; 32 mM SAM, prepared by diluting New England Biolabs catalog #B9003S with Nuclease-Free Water), and Vaccinia Capping Enzyme (540 μl; Hongene catalog #ON-028) were added, and the resultant mixture was incubated at 37° C. for 4 hours. Then, 10× capping buffer (90 μl), 20 mM SAM (270 μl) and 2′-O-methyltransferase (540 μl; Hongene catalog #ON-014) were added thereto, and the resultant mixture was incubated at 37° C. for 4 hours. 8 M LiCl solution (6300 μl) was further added, and the mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water and passed through two-connected columns of reversed phase high performance liquid chromatography (Chromolith Semi-Prep (Merck catalog #1.52016.0001)) with 5% acetonitrile, 400 mM triethylamine acetate (pH 7.0)/25% acetonitrile, and 400 mM triethylamine acetate (pH 7.0, 80° C.) as eluents to thereby purify the mRNA of interest.
The resultant mRNA has the sequence as shown in SEQ ID NO: 6. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
(4) Preparation of HPV16 E6-E7 Fusion10 Opt2 mRNA-001 by In Vitro Transcription
500 μg/ml template DNA from Example 6-(2) (200 μl), 100 mM ATP (300 μl, Hongene catalog #R1331), 100 mM GTP (300 μl, Hongene catalog #R2331), 100 mM CTP (300 W, Hongene catalog #R3331), 100 mM N1-methylpseudouridine-5′-triphosphate (300 μl, Hongene catalog #R5-027), Nuclease-Free Water (1400 μl, Applied-Bio catalog #AM9937), T7 Transcription 5× buffer (800 μl) (400 mM HEPES-KOH (pH 7.5), 80 mM MgCl2, 10 mM spermidine, 200 mM DTT), Enzyme mix, and T7 RNA Polymerase (400 μl, Promega catalog #P137X) were mixed and incubated at 37° C. for 8 hours. RQ1 RNase-Free DNase (100 μl, Promega catalog #M6101) was added, and the resultant mixture was incubated at 37° C. for 15 minutes. 8 M LiCl solution (2000 μl, Sigma-Aldrich catalog #L7026) was further added, and the resultant mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water. To this solution (1590 μl; corresponding to 6000 μg of RNA on the basis of UV absorbance), Nuclease-Free Water (2610 μl) was added. The resultant solution was heated at 70° C. for 10 minutes and then cooled on ice for 10 minutes. To this solution, 600 μl of 10× capping buffer (500 mM Tris-HCl (pH 8.0), 50 mM KCl, 10 mM MgCl2, 50 mM DTT), 20 mM GTP (300 μl; 100 mM GTP, prepared by diluting Hongene catalog #R2331 with Nuclease-Free Water), 20 mM SAM [300 μl; prepared by dissolving S-adenosyl-L-methionine disulfate tosylate (OX-CHEM catalog #AX8250818)-in 10% ethanol solution of 0.005 M sulfuric acid] and Vaccinia Capping Enzyme (600 μl; Hongene catalog #ON-028) were added, and the resultant mixture was incubated at 37° C. for 4 hours. Then, 10× capping buffer (100 μl), 20 mM SAM (300 μl) and 2′-O-methyltransferase (600 μl; Hongene catalog #ON-014) were added thereto, and the resultant mixture was incubated at 37° C. for 4 hours. 8 M LiCl solution (7000 μl) was further added, and the mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water and passed through a column of reversed phase high performance liquid chromatography (Chromolith Performance (Merck catalog #1.02129.0001) with 5% acetonitrile, 400 mM triethylamine acetate (pH 7.0)/25% acetonitrile, 400 mM triethylamine acetate (pH 7.0, 45° C.) as eluents to thereby purify the mRNA of interest.
The resultant mRNA has the sequence as shown in SEQ ID NO: 6. It was confirmed by the analysis with LabChip GX Touch HT mRNA StdSens (PerkinElmer catalog #CLS960010) that this mRNA has an anticipated nucleitoide length.
The mRNA was obtained in the same manner as described in Example 6-(3) except that 100 mM 5-Me-CTP and 100 mM 5-methyluridine triphosphate were used instead of 100 mM CTP and 100 mM N1-methylpseudouridine-5′-triphosphate, respectively. The resultant mRNA has the sequence as shown in SEQ ID NO: 6. It was confirmed by the analysis with Experion RNA StdSens that this mRNA has an anticipated nucleotide length.
(1) Preparation of Nucleic Acid Lipid Particles Encapsulating mRNA
Distearoyl phosphatidylcholine (1,2-Distearoyl-sn-glycero-3-phosphocholine; hereinafter, designsated as DSPC; NOF CORPORATION), cholesterol (hereinafter, designsated as Chol; Sigma-Aldrich, Inc.), (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate (a compound disclosed in Example 23 in WO2015/005253) (hereinafter, designsated as LP) and 1,2-dimyristoyl-sn-glycerol-3-methoxypolyethylene glycol in which the polyethylene glycol part has the molecular weight of about 2000 (hereinafter, designsated as PEG-DMG; NOF CORPORATION) were dissolved in ethanol so that a molar ratio of DSPC:Chol:LP:PEG-DMG is 10:43.5:45:1.5 to give a total lipid concentration of 5 mM.
On the other hand, HPV16 fusion2 mRNA-001 obtained in Example 1 was diluted with 20 mM citrate buffer (pH 4.0) to prepare a solution of 51.8 μg/ml.
The lipid solution and the mRNA solution described above were mixed to give a volume ratio of 1:3 in a micro flow channel using NanoAssemblr BenchTop (Precision Nanosystems Inc.) to thereby obtain a crude dispersion of nucleic acid lipid particles. This dispersion was dialyzed against about 25 to 50 volumes of phosphate buffer (pH 7.4) for 12 to 18 hours (Float-A-Lyzer G2, MWCO: 1,000 kD, Spectra/Por) to thereby remove ethanol and obtain a purified dispersion of nucleic acid lipid particles encapsulating mRNA.
LP was synthesized according to the method described in Example 23 of WO2015/005253.
(2) Characterization of Nucleic Acid Lipid Particles Encapsulating mRNA
The dispersion containing the nucleic acid lipid particles prepared in (1) above was characterized. Methods of characterization of each property will be described below.
(2-1) Encapsulation Rate of mRNA
Encapsulation rate of mRNA was measured with Quant-iT RiboGreen RNA Assay kit (Invitrogen) according to the attached protocol with necessary modifications.
Briefly, mRNA in the dispersion of nucleic acid lipid particles was quantified in the presence or absence of 0.015% Triton X-100 surfactant, and then encapsulation rate was calculated by the following formula.
{[amount of mRNA in the presence of surfactant]−[amount of mRNA in the absence of surfactant]}/[amount of mRNA in the presence of surfactant]}×100(%).
(2-2) Ratio of mRNA and Lipids
The amount of mRNA in the dispersion of nucleic acid lipid particles was measured by reversed phase chromatography (System: Agilent 1100 series; Column: Bioshell A400 Protein C4 (10 cm×4.6 mm, 3.4 μm) (SUPELCO); Buffer A: 0.1 M triethylamine acetate (pH 7.0); Buffer B: acetonitrile; (B %): 5-50% (0-15 min); Flow Rate: 1 ml/min; Temperature: 70° C.; Detection: 260 nm).
The amount of each lipid in the dispersion of nucleic acid lipid particles was measured by reversed phase chromatography (System: DIONEX UltiMate 3000; Column: XSelect CSH (50 mm×3 mm, 5 μm) (Thermo Fisher Scientific); Buffer A: 0.2% formic acid; Buffer B: 0.2% formic acid, methanol; (B %): 75-95% (0-15 min), 95% (15-17 min); Flow Rate: 0.4 ml/min; Temperature: 50° C.; Detection: Corona CAD (Charged Aerosol Detector)).
The ratio of the total lipid to mRNA was calculated by the following formula.
[Total lipid concentration]/[mRNA concentration](wt/wt)
The mean particle size of nucleic acid lipid particles in a dispersion was measured with Zeta Potential/Particle Sizer NICOMP™ 380ZLS (Particle Sizing Systems). The mean particle size in the dispersion represents the volume mean particle sizes together with its deviation.
The results are shown in Table 1.
The nucleic acid lipid particles encapsulating the mRNA described in Example 2, 3, 4, 5 or 6 were prepared and characterized in the same manner as described in Example 8, except that the lipid composition used had a molar ratio of 12.5:41:45:1.5 for DSPC:Chol:LP:PEG-DMG. The results are shown in Table 1.
The nucleic acid lipid particles encapsulating the mRNA described in Example 2, 4, 6 or 7 were prepared and characterized in the same manner as described in Example 8, except that the lipid composition used had a molar ratio of 12.5:41:45:1.5 for DSPC:Chol:LP:PEG-DMG. The results are shown in Table 1.
The nucleic acid lipid particles encapsulating the mRNA described in Example 2 were prepared and characterized in the same manner as described in Example 8, except that the lipid composition used had a molar ratio of 12.5:41:45:1.5 for DSPC:Chol:LP:PEG-DMG and that the ratio of the total lipid weight to mRNA weight was 25. The results are shown in Table 1.
The nucleic acid lipid particles encapsulating the mRNA described in Example 2 were prepared and characterized in the same manner as described in Example 8, except that the lipid composition had a molar ratio of 12.5:41:45:1.5 for DSPC:Chol:LP:PEG-DMG, and the ratio of the total lipid weight to mRNA weight was 30. The results are shown in Table 1.
The nucleic acid lipid particles encapsulating the mRNA described in Example 2 were prepared and characterized in the same manner as described in Example 8. The results are shown in Table 1.
(1) Preparation of Nucleic Acid Lipid Particles Encapsulating mRNA
The nucleic acid lipid particles encapsulating an mRNA having the coding region of OVA (ovalbumin) as shown in SEQ ID NO: 7 were prepared in the same manner as described in Example 8-(1). However, instead of PEG-DMG, N-[methoxy poly(ethylene glycol)2000 carbamoyl]-1,2-dimyristyloxypropyl-3-amine in which the polyethylene glycol part has the molecular weight of about 2000 (hereinafter, designated as PEG-C-DMA; corresponding to compound 12 disclosed in Journal of Controlled Release 112 (2006) 280-290) was used, and the lipid composition of the nucleic acid lipid particles had a molar ratio of 10:38.5:50:1.5 for DSPC:Chol:LP:PEG-C-DMA.
(2) Characterization of Nucleic Acid Lipid Particles Encapsulating mRNA
A dispersion containing the nucleic acid lipid particles prepared in (1) above was characterized. Methods of characterization of each property will be described below.
(2-1) Encapsulation Rate of mRNA
Encapsulation rate of mRNA was measured with Quant-iT RiboGreen RNA Assay kit (Invitrogen) according to the attached protocol with necessary modifications.
Briefly, mRNA in the dispersion of the nucleic acid lipid particles was quantified in the presence or absence of 0.015% Triton X-100 surfactant, and then encapsulation rate was calculated by the following formula.
{[amount of mRNA in the presence of surfactant]−[amount of mRNA in the absence of surfactant]}/[amount of mRNA in the presence of surfactant]}×100(%)
(2-2) Ratio of mRNA and Lipids
The amount of mRNA in the presence of surfactant in (2-1) was regarded as the amount of mRNA in the dispersion of the nucleic acid lipid particles.
The amount of phospholipids in the dispersion of the nucleic acid lipid particles was measured with Phospholipids C-test Wako kit (Fuji Film Wako Purechemicals) according to the attached protocol with necessary modification. Briefly, the amount of phospholipids in the dispersion was measured in the presence of 2% Triton X-100 surfactant.
The amounts of cholesterol and LP in the dispersion of the nucleic acid lipid particles were measured by reversed phase chromatography (System: DIONEX UltiMate 3000; Column: Chromolith Performance RP-18 endcapped 100-3 monolithic HPLC-column (Merck, Cat. #1.52001.0001); Buffer A: 0.01% trifluoroacetate; Buffer B: 0.01% trifluoroacetate, methanol; (B %): 82-97% (0-17 min); Flow Rate: 2 ml/min; Temperature: 50° C.; Detection: Corona CAD (Charged Aerosol Detector)).
The amount of total lipid and the ratio of lipid components constituting the nucleic acid lipid particles was calculated from measured values of phospholipids, cholesterol and LP.
The ratio of the total lipid to mRNA was calculated by the following formula.
[total lipid concentration]/[mRNA concentration](wt/wt)
The mean particle size of nucleic acid lipid particles was measured with Zeta Potential/Particle Sizer NICOMP™ 380ZLS (Particle Sizing Systems). The mean particle size represents the volume mean particle sizes together with its deviation.
The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA (ovalbumin) as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 10:35:50:5 for DSPC:Chol:LP:PEG-C-DMA. The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 10:23.5:65:1.5 for DSPC:Chol:LP:PEG-C-DMA. The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 10:48.5:40:1.5 for DSPC:Chol:LP:PEG-C-DMA. The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 5:43.5:50:1.5 for DSPC:Chol:LP:PEG-C-DMA The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 15:33.5:50:1.5 for DSPC:Chol:LP:PEG-C-DMA. The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the mRNA having the coding region of OVA as shown in SEQ ID NO: 7 were prepared and characterized in the same manner as described in Example 21, except that the lipid composition used had a molar ratio of 53.5:45:1.5 for Chol:LP:PEG-C-DMA. The results are shown in Table 2.
The nucleic acid lipid particles encapsulating the HPV mRNA described in Example 4 were prepared and characterized in the same manner as described in Example 8, except that dioleoyl phosphatidylcholine (1,2-Dioleoyl-sn-glycero-3-phosphocholine; hereinafter, designated as DOPC: NOF CORPORATION) was used instead of DSPC, so that the lipid composition had a molar ratio of 10:43.5:45:1.5 for DOPC:Chol:LP:PEG-DMG. The results are shown in Table 3.
The nucleic acid lipid particles encapsulating the HPV mRNA described in Example 4 were prepared and characterized in the same manner as described in Example 8, except that DOPC was used instead of DSPC, so that the lipid composition had a molar ratio of 15:38.5:45:1.5 for DOPC:Chol:LP:PEG-DMG. The results are shown in Table 3.
The nucleic acid lipid particles encapsulating the HPV mRNA described in Example 4 were prepared and characterized in the same manner as described in Example 8, except that dioleoyl phosphatidyl ethanolamine (1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine; hereinafter, designated as DOPE; NOF CORPORATION) was used instead of DSPC, so that the lipid composition had a molar ratio of 10:43.5:45:1.5 for DOPE:Chol:LP:PEG-DMG. The results are shown in Table 3.
The nucleic acid lipid particles encapsulating the HPV mRNA described in Example 4 was prepared and characterized in the same manner as described in Example 8, except that DOPE was used instead of DSPC, so that the lipid composition had a molar ratio had a molar ratio of 15:38.5:45:1.5 for DOPE:Chol:LP:PEG-DMG. The results are shown in Table 3.
The nucleic acid lipid particles encapsulating the HPV mRNA described in Example 4 was prepared and characterized in the same manner as described in Example 8, except that the lipid composition used had a molar ratio of 12.5:41:45:1.5 for DSPC:Chol:LP:PEG-DMG. The results are shown in Table 3.
A plasmid was constructed in order to prepare a template DNA for IVT. Briefly, a DNA fragment (SEQ ID NO: 10) comprising GCTAGC (NheI site), T7 promoter sequence, 5′-UTR sequence of human β-globin, KOZAK sequence, ORF for IgE leader sequence-HPV18 E6-furin cleavage site-HPV18 E7, 3′-UTR sequence of human β-globin, polyA tail and ACTAGT (SpeI site) was prepared by ligation in this order and then introduced into a plasmid to generate a plasmid of interest (pMA-HPV18_fusion1_opt1).
The plasmid generated in Example 33-(1) (350 μg) was dissolved in Nuclease-Free Water (3080 μl, Thermo Fisher catalog #AM9937). To this solution, 10× CutSmart Buffer (350 μl, New England Biolabs catalog #B7204S) and SpeI-HF (70 μl, New England Biolabs catalog #R3133L) were added, and the resultant mixture was incubated at 37° C. for 2 hours and then at 65° C. for 20 minutes. 7.5 M ammonium acetate (1750 μl) and ethanol (10500 μl) were added and mixed with the incubated solution, which was then left to stand overnight at −80° C. After centrifugation (−10° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (−10° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was collected and air-dried. TE-Buffer was added to the air-dried precipitate to prepare a template DNA solution of 500 μg/ml.
(3) Preparation of HPV18 E6-E7 Fusion1 Opt1 mRNA-001 by In Vitro Transcription
500 μg/ml template DNA from Example 33-(2) (150 μl), 100 mM CleanCap AG (150 μl, TriLink catalog #T-7113), 100 mM ATP (150 μl, Hongene catalog #R1331), 100 mM GTP (150 μl, Hongene catalog #R2331), 100 mM CTP (150 μl, Hongene catalog #R3331), 100 mM N1-Me-Pseudo UTP (150 μl, Hongene catalog #R5-027), Nuclease-Free Water (1200 μl, Thermo Fisher catalog #AM9937), T7 Transcription 5× buffer (600 μl, Promega catalog #P140X), Enzyme mix, and T7 RNA Polymerase (300 μl, Promega catalog #P137X) were mixed, and-incubated at 37° C. for 4 hours. RQ1 RNase-Free DNase (75 μl, Promega catalog #M6101) was added and the resultant mixture was incubated at 37° C. for 15 minutes. 8 M LiCl solution (1500 μl, Sigma-Aldrich catalog #L7026) was further added, and the resultant mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water, followed by purification using RNeasy Maxi kit (Qiagen catalog #75162) according to the attached manual. A portion of the eluate obtained (11.0 ml; corresponding to 9813 μg of RNA on the basis of UV absorbance), Nuclease-free water (537 μl), and rApid Alkaline Phosphatase and the buffer for (1500 μl) for this enzyme (Roche catalog #04 898 141 001) were mixed. The mixture was incubated at 37° C. for 30 minutes and then at 75° C. for 3 minutes. 8 M LiCl solution (15000 μl) was added, and the resultant mixture was left to stand for 3 hours at −20° C. After centrifugation (−8° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (−8° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water and passed through a column of reversed phase high performance liquid chromatography (YMC Triart-C8 5 μm 10×150 mm (YMC #TO12S05-1510WT)) with 5% acetonitrile, 400 mM triethylamine acetate (pH 7.0)/25% acetonitrile, 400 mM triethylamine acetate (pH 7.0, 75° C.) as eluents to thereby purify the mRNA of interest.
The resultant mRNA has the sequence as shown in SEQ ID NO: 11. It was confirmed by the analysis with LabChip GX Touch Standard RNA Reagent Kit (PerkinElmer catalog #CLS960010) that this mRNA has an anticipated nucleotide length.
The mRNA was obtained in the same manner as described in Example 33-(3) except that 100 mM 5-Me-CTP (Hongene catalog #R3-029) and 100 mM 5-methyluridine triphosphate were used instead of 100 mM CTP and 100 mM N1-Me-Pseudo UTP, respectively.
The resultant mRNA has the sequence as shown in SEQ ID NO: 11. It was confirmed by the analysis with LabChip GX Touch Standard RNA Reagent Kit (PerkinElmer catalog #CLS960010) that this mRNA has an anticipated nucleotide length.
A plasmid was constructed in order to prepare a template DNA for IVT. Briefly, a DNA fragment (SEQ ID NO: 12) containing GCTAGC (NheI site), T7 promoter sequence, 5′-UTR sequence of β-globin, KOZAK sequence, ORF for IgE leader sequence-HPV18 E6-furin cleavage site-HPV18 E7, 3′-UTR sequence of $-globin, polyA tail and ACTAGT (SpeI site) was prepared by ligation in this order and then introduced into a plasmid to generate the plasmid of interest (pMA-HPV18_fusion1_opt2).
The plasmid generated in Example 35-(1) (400 μg) was dissolved in Nuclease-Free Water (3520 μl, Thermo Fisher catalog #AM9937). To this solution, 10× CutSmart Buffer (400 μl, New England Biolabs catalog #B7204S) and SpeI-HF (80 μl, New England Biolabs catalog #R3133L) were added, and the resultant mixture was incubated at 37° C. for 2 hours and then at 65° C. for 20 minutes. 7.5 M ammonium acetate (1750 μl) and ethanol (10500 μl) were added and mixed with the incubated solution, which was then left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the resultant precipitate was collected and air-dried. TE-Buffer was added to the air-dried precipitate to prepare a template DNA solution of 500 μg/ml.
(3) Preparation of HPV16 E6-E7 Fusion1 Opt2 mRNA-001 by In Vitro Transcription
500 μg/ml template DNA from Example 35-(2) (150 μl), 100 mM ATP (225 μl, Hongene catalog #R1331), 100 mM GTP (225 μl, Hongene catalog #R2331), 100 mM CTP (225 μl, Hongene catalog #R3331), 100 mM N1-Me-Pseudo UTP (225 μl, Hongene catalog #R5-027), Nuclease-Free Water (1050 μl, Thermo Fisher catalog #AM9937), T7 Transcription 5× buffer (600 μl, Promega catalog #P140X), Enzyme mix, and T7 RNA Polymerase (300 μl, Promega catalog #P137X) were mixed, and incubated at 37° C. for 4 hours. RQ1 RNase-Free DNase (75 μl, Promega catalog #M6101) was added, and the resultant mixture was incubated at 37° C. for 15 minutes. 8 M LiCl solution (1500 μl, Sigma-Aldrich catalog #L7026) was further added, and the resultant mixture was left to stand overnight at −20° C. After centrifugation (4° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (4° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water. Nuclease-Free Water (2780 μl) was added to a portion of this solution (2470 μl; corresponding to 7500 μg of RNA on the basis of UV absorbance). The resultant solution was heated at 70° C. for 10 minutes and then cooled on ice for 5 minutes. 750 μl of 10× capping buffer (500 mM Tris-HCl (pH 8.0), 50 mM KCl, 10 mM MgCl2, 50 mM DTT), 20 mM GTP (375 μl; 100 mM GTP; prepared by diluting Hongene catalog #R2331 with Nuclease-Free Water), 20 mM SAM (375 μl; 32 mM SAM; prepared by diluting New England Biolabs catalog #B9003S with Nuclease-Free Water), and Vaccinia Capping Enzyme (750 μl; Hongene catalog #ON-028) were added thereto, and the resultant mixture was incubated at 37° C. for 4 hours. Then, 10× capping buffer (125 μl), 20 mM SAM (375 μl), and 2′-O-methyltransferase (750 μl; Hongene catalog #ON-014) were added thereto, and the resultant mixture was incubated at 37° C. for 4 hours. 8 M LiCl solution (8750 μl) was further added, and the resultant mixture was left to stand overnight at −20° C. After centrifugation (−8° C., 4000×g, 30 minutes), the supernatant was discarded and 70% ethanol was added to the precipitate. After centrifugation (−8° C., 4000×g, 10 minutes), the supernatant was discarded and the precipitate obtained was air-dried. The air-dried precipitate was dissolved in Nuclease-Free Water and passed through a column of reversed phase high performance liquid chromatography (YMC Triart-C8 5 μm 10×150 mm (YMC #TO12S05-1510WT) with 5% acetonitrile, 400 mM triethylamine acetate (pH 7.0)/25% acetonitrile, and 400 mM triethylamine acetate (pH 7.0, 75° C.) as eluents to thereby purify the mRNA of interest.
The resultant mRNA has the sequence as shown in SEQ ID NO: 13. It was confirmed by the analysis with LabChip GX Touch Standard RNA Reagent Kit (PerkinElmer catalog #CLS960010) that this mRNA has an anticipated nucleotide length.
The mRNA was obtained in the same manner as described in Example 35-(3) except that 100 mM 5-Me-CTP (Hongene catalog #R3-029) and 100 mM 5-methyluridine triphosphate were used instead of 100 mM CTP and-100 mM N1-Me-Pseudo UTP, respectively.
The resultant mRNA has the sequence as shown in SEQ ID NO: 13. It was confirmed by the analysis with LabChip GX Touch Standard RNA Reagent Kit (PerkinElmer catalog #CLS960010) that this mRNA has an anticipated nucleotide length.
(1) Preparation of Nucleic Acid Lipid Particles Encapsulating mRNA
Nucleic acid lipid particles encapsulating mRNA were prepared and characterized in the same manner as described in Example 8, except that the mRNAs from Examples 33 to 36 were used instead of the mRNA of Example 1. The molar ratio of the lipids used for each preparation is indicated in Table 4. The results are shown in Table 4.
(1) Preparation of Nucleic Acid Lipid Particles Encapsulating mRNA
Nucleic acid lipid particles encapsulating the mRNA of Example 4 were prepared in the same manner as described in Example 8, except that (7R,9Z)-18-({[3-(dimethylamino)prypyloxy]carbonyl}oxy)octacosa-9-ene-7-yl acetate (a compound disclosed in Example 28 in WO2015/005253) (hereinafter, designated as LP2) was used instead of (7R,9Z,26Z,29R)-18-({[3-(dimethylamino)propoxy]carbonyl}oxy)pentatriaconta-9,26-diene-7,29-diyl diacetate. The molar ratio of the lipids used is indicated in Table 5.
LP2 was synthesized according to the method described in Example 28 of-WO2015/005253.
(2) Characterization of Nucleic Acid Lipid Particles Encapsulating mRNA
Nucleic acid lipid particles encapsulating mRNA were characterized in the same manner as described in Example 8, and the amount of mRNA encapsulated was analyzed as described below.
Briefly, a dispersion of nucleic acid lipid particles was dissolved in 90% methanol, and the amount of mRNA was measured with a UV-visible spectrophotometer (LAMBDA™ 465, PerkinElmer, Inc.). Then, the mRNA concentration was calculated by the following formula.
{[absorbance at 260 nm]−[absorbance at 350 nm]}×40×dilution rate (μg/ml)
The results are shown in Table 5.
A plasmid for the expreesion of HPV16 E6-E7 fusion protein was constructed with reference to J. Yan et al., Vaccine 27 (2009) 431-440. As the coding region, the sequence registered under GenBank Accession Number: Fj229356 was used.
The results shown in Table 1 clearly reveal that more than 90% of mRNA is encapsulated in lipid particles with mean particle sizes of approximately 100 to 130 nm.
The results shown in Table 2 clearly reveal that more than 75% of mRNA is encapsulated in lipid particles with mean particle sizes of approximately 70 to 170 nm.
The results shown in Table 3 clearly reveal that more than 95% of mRNA is encapsulated in lipid particles with-mean particle sizes of approximately 80 to 110 nm.
The results shown in Table 4 clearly reveal that more than 95% of mRNA is encapsulated in lipid particles with mean particle sizes of approximately 90 to 130 nm.
The results shown in Table 5 clearly reveal that more than 95% of mRNA is encapsulated in lipid particles witn mean particle sizes of approximately 70 to 120 nm.
Expression Levels of HPV16 E7 Vaccine Antigen in Cultured Cells Transfected with Nucleinc Acid Particles Encapsulating mRNA (
HEK293T cells (human embryonic kidney cell line) were seeded in 96-well plates at 2×104 cells/well and cultured overnight at 37° C. in an atmosphere of 5% CO2. Subsequently, the nucleic acid lipid particles encapsulating mRNA prepared in Examples 14 to 17 were added respectively to the HEK293T cells at 0.3 to 10 μg/ml as final mRNA concentration. The cells were cultured at 37° C. in an atmosphere of 5% CO2 for 48 hours. After the 96-well plates were left to stand for 1 hour at 4° C., each well was washed 3 times with 300 μl of PBS (−) containing 0.05% Tween 20 (PBST). Subsequently, HPV16 E7 protein (16E7) coated on the wells of the plates was reacted with horse radish peroxidase (HRP)-labeled anti-16E7 antibody at room temperature for 2 hours. After washing the wells 3 times with PBST, HRP substrate was added to the wells for the detection of 16E7. Expression levels of 16E7 protein were calculated by subtracting the absorbance at 540 nm (background absorption) from that at 450 nm in each well.
C57BL/6J mice were purchased from CLEA Japan. Every animal experiment was conducted according to the institutional guideline of the National Institute of Biomedical Innovation, Health and Nutrition, Japan. All treatments of animals was conducted under anesthesia by inhalatiom of isoflurane or by subcutaneous administration of Ketalar/Seractal.
Four to ten microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was inoculated into the gastrocnemius muscle of each 7 week-old C57BL/6 mouse twice 10 days apart. Administration of plasmid DNA into the gastrocnemius muscle was perfomed by electroporation at 40 μg of DNA per mouse. The conditions for the electroporation were as follows: 30 V; 50 ms ON/100 ms OFF; and 3 cycles. Administration by electroporation was also conducted twice 10 days apart. Peripheral blood was collected in the presence of heparin at one week after the final immunization, while the spleen was collected at one or two weeks after the final immunization to prepare peripheral blood mononuclear cells (PBMCs) and splenocytes for evaluation. The induction levels of cytotoxic T lymphocytes (CTL) specific to HPV16 E7 vaccine antigen in PBMCs and splenocytes were measured by FACS through immunostaining with antibodies to T cell surface markers and tetramer complex of 16E7 epitope.
Ten microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was inoculated into the gastrocnemius muscle of each 7 week-old C57BL/6 mice twice 10 days apart. Peripheral blood was collected in the presence of heparin at one week after the final immunization, and plasma was prepared. Anti-HPV16 E7 IgG titer of plasma was measured by ELISA. ELISA was briefly conducted as followed. The 16E7 recombinant protein was coated on each well of 96-well plates at a concentration of 0.5 μg/ml at 4° C. overnight. Simultaneously, a dilution series of mouse IgG protein for standard curve was also prepared and coated on the same plates. Subsequently, the wells were washed 3 times with PBST and then blocked with 1% BSA-containing PBST (1% BSA/PBST) for 1 hour. Serial dilutions of plasma samples were prepared with 1% BSA/PBST, added to 16E7-immobilized wells, and reacted at room temperature for 2 hours. One percent of BSA in PBST was added to the wells for mouse IgG standard curves and reacted in the same manner. After washing 3 times with PBST, HRP-labeled anti-mouse IgG antibody was added to each well and reacted at room temperature for 1 hour. After washing 3 times with PBST, HRP substrate was added to each well for color development, followed by addition of 1N sulfuric acid solution to terminate the color development. The concentration of mouse IgG bound to 16E7 in each well was calculated using standard curves after subtracting absorbance at 540 nm as a background-from absorbance at 450 nm.
Regression of TC-1 Tumoer Cell Growth in C57BL/6 Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA (
TC-1 cells which was established from lung epithelial cells of C57BL/6 mice and express HPV16 E6 and E7 were transplanted subcutaneously to the lateral abdomen of C57BL/6 mice, and tumor size was measured over time. The transplantation site was shaved prior to transplantation of TC-1 cells, and 1×105 of TC-1 cells were transplanted into each mouse. Ten microgram mRNA of the nucleic acid lipid particles encapsulating the mRNA of Example 20 was administered intramuscularly into each mouse at 8 days after-the transplantation of TC-1 cells. Administration of antibodies to deplete CD4 positive cells or CD8 positive cells was conducted at 2 days before the administration of the nucleic acid lipid particles encapsulating mRNA.
A hundred microgram of anti-CD4 antibody (GK1.5) or anti-CD8 antibody (53-6.72) were administered intraperitoneally to each 7 week-old C57BL/6 mice for 3 consecutive days at 2 days before the administration of nucleic acid lipid particles encapsulating mRNA. At 3 days after antibody administration, 10 μg mRNA of the nucleic acid lipid particles encapsulating mRNA from Example 20 was administered into the gastocnemius muscle of each mouse.
Fifteen microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was administered subcutaneously to the tail base of each 7 week-old C57BL/6 mouse 2 weeks apart. One week after the final immunization, peripheral blood was collected and serum was prepared. Anti-OVA IgG titer in plasma was measured by ELISA. ELISA was briefly conducted as followed. OVA recombinant protein was coated at a concentration of 1 μg/ml on each well of 96-well plates at 4° C. overnight. Simultaneously, a dilution series of mouse IgG protein was also coated on the same plates for standard curves. Subsequently, the wells were washed 3 times with PBST and then blocked with 1% BSA-containing PBST (1% BSA/PBST) for 1 hour. Serial dilutions of serum samples were prepared with 1% BSA/PBST, were added to OVA-immobilized wells, and were reacted at room temperature for 2 hours. One percent of BSA in PBST was added to the wells for mouse IgG standard curves and reacted in the same manner. After washing with PBST 3 times, HRP-labeled anti-mouse IgG antibody was added to each well and reacted at room temperature for 1 hour. After washing with PBST 3 times, HRP substrate was added to each well for color development, followed by addition of 1N sulfuric acid solution to terminate the color development. The concentration of mouse anti-OVA IgG titer in each well was calculated using standard curves after subtracting absorbance at 540 nm as a background from absorbance at 450 nm.
OVA-Specific Cytokine Production from T Cells (
Fifteen microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was administered subcutaneously to the tail base of each 7 week-old C57BL/6 mouse twice 2 weeks apart. One week after the final immunization, the spleen was collected and splenocytes were prepared. Splenocytes were seeded in 96-well culture plates, were stimulated with MHC class I-restricted epitope peptide of OVA antigen or with OVA protein, and then were cultured for 24 hours. The IFN-γ level in culture supernatant was measured by cytokine ELISA.
HPV18E6- or HPV18E7-Specific Cytokine Production from T Cells (
C57BL/6J mice were purchased from CLEA Japan. Every treatment of animals was conducted under anesthesia by inhalation of isoflurane.
Five microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was administered into the gastrocnemius muscle of each 6 week-old C57BL/6 mouse twaice 2 weeks apart. One week after the final immunization, the spleen was collected and splenocytes were prepared. Splenocytes were treated with HPV18 E6 pool peptide (JPT Peptide Technologies, catalog #PM-HPV18-E6). IFN-γ level in culture supernatant after 48-hour culture was measured by cytokine ELISA.
C57BL/6J mice were purchased from CLEA Japan. Every treatment of animals was conducted under anesthesia by inhalation of isoflurane.
Five microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was administered into the gastrocnemius muscle of each 6 week-old C57BL/6J mouse twice 2 weeks apart. One week after the final immunization, the spleen was collected and splenocytes were prepared. The levels CTL specific to E7 of HPV16 genotype (HPV16E7) in splenocytes were measured by flow cytometry through the immunostaining with antibodies to T lymphocyte surface markers and complex of MHC class I with HPV16E7 epitope.
HPV16 E6- or E7-Specific Cytokine Production from T Cells (
Five microgram mRNA of Nucleic acid lipid particles encapsulating mRNA was administered into the gastrocnemius muscle of each 6 week-old C57BL/6J mouse twice 2 weeks apart. One week after the final immunization, the spleen was collected and splenocytes were prepared. Splenocytes were seeded in 96-well culture plates and cultured for 24 hours under the treatment with MHC class I-restricted epitope peptide of HPV16E7. Subsequently, IFN-γ levels in the culture supernatant was measured by cytokine ELISA.
Expression Levels of HPV16 E7 from HEK293T Cells Transfected with Nucleic Lipid Particles Encapsulating mRNA of Examples 14 to 17 (
HPV16 E7 expression levels from HEK293T cells transfected with nucleic lipid particles encapsulating mRNA in cultured cells were evaluated. The results are shown in
Comparison of CTL Levels Between the DNA Vaccine and Nucleic Acid Lipid Particles Encapsulating mRNA (
Construction of the DNA vaccine is described in Reference Example 1. The levels of CTL specific to HPV16E7 in mice administered with the DNA vaccine or the nucleic acid lipid particles encapsulating mRNA of Examples 9, 11 and 13 were evaluated. The results are shown in
The CTL Levels in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 14 to 17 (
16E7-specific CTL levels in C57BL/6 mice administered with four types of nucleic acid lipid particles encapsulating mRNA were examined. The results are shown in
Importance of CD4 Positive Cells but not CD8 Positive Cells for the Antibody Responses in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA (
Antibody responses were examined in mice administered with nucleic acid lipid particles encapsulating mRNA under depletion of CD4 positive cells or CD8 positive cells after transplantation of TC-1 tumor cells. The results are shown in
Importance of CD4 Positive Cells and CD8 Positive Cells for the CTL Induction in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA (
The levels of CTL specific to 16E7 in mice administered with nucleic acid lipid particles encapsulating mRNA were examined under depletion of CD4 positive cells or CD8 positive cells after transplantation of TC-1 tumor cells. The results are shown in
Importance of CD4 Positive Cells and CD8 Positive Cells for TC-1 Tumor Regression Effect in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA (
TC-1 tumor regression effect in mice administered with nucleic acid lipid particles encapsulating mRNA were examined under depletion of CD4 positive cells or CD8 positive cells after transplantation of TC-1 tumor cells. The results are shown in
Anti-OVA Antibody Responses in Mice Administered with by Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 21 to 27 with Different Lipid Compositions (
The nucleic acid lipid particles encapsulating mRNA of Examples 21 to 27 were administered intramuscularly to C57BL/6 mice. One week after the final immunization, anti-OVA antibody responses in blood were examined. The results are shown in
OVA-Specific IFN-γProduction from Splenocytes of Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 21 to 27 with Different Lipid Compositions (
The nucleic acid lipid particles encapsulating mRNA of Examples 21 to 27 were administered intramuscularly to C57BL/6 mice. One week after the final immunization, levels of OVA-specific-cytokines from splenocytes were examined. The results are shown in
CTL Levels in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 28 to 32 with Different Phospholipid Species and its Content (
The levels of HPV16E7-specific CTL in C57BL/6 mice administered with five types of nucleic acid lipid particles encapsulating mRNA were examined. The results are shown in
HPV18E6-Specific Cytokine Production from Splenocytes in Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 37 to 40 with Different mRNA Modification (
Nucleic acid lipid particles encapsulating mRNA of Examples 37 to 40 were administered intramuscularly to C57BL/6 mice. One week after the final immunization, the levels of HPV18 E6-specific T cell cytokine production from splenocytes were examined. The results are shown in
CTL Levels in Mice Administered with of Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 41 to 52 with Different Lipid Composition Ratios (
Nucleic acid lipid particles encapsulating mRNA of Examples 41 to 52 were administered intramuscularly to C57BL/6 mice. One week after the final immunization, the levels of HPV16E7-specific CTL in splenocytes were evaluated. The results are shown in
HPV16E7-Specific IFN-γ Production from Splenocytes of Mice Administered with Nucleic Acid Lipid Particles Encapsulating mRNA of Examples 41 to 52 with Different Lipid Composition Ratios (
Nucleic acid lipid particles encapsulating mRNA of Examples 41 to 52 were administered intramuscularly to C57BL/6 mice. One week after the final immunization, levels of HPV16 E7-specific T cell cytokine production from splenocytes were examined. The results are shown in
All publications, patents and patent applications cited herein are incorporated herein by reference in their entirety.
The present invention is applicable to prevention and/or treatment of infections with human papillomavirus.
A: transcription start site: nucleotide number 25
G: transcription start site: nucleotide number 25
| Number | Date | Country | Kind |
|---|---|---|---|
| 2019-207001 | Nov 2019 | JP | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/JP2020/042405 | 11/13/2020 | WO |
