Claims
- 1. An isolated nucleic acid molecule comprising:
a) an isolated nucleic acid molecule encoding an amino acid sequence of SEQ ID NO:2, and conservatively modified and polymorphic variants thereof; b) an isolated nucleic acid molecule which selectively hybridizes at high stringency to a nucleic acid molecule of (a); c) complementary sequence of nucleic acid molecules of (a) or (b); d) an isolated nucleic acid molecule which is the reverse complement of (a), (b) or (c); e) an isolated nucleic molecule encoding a functional portion of the polypeptide of SEQ ID NO:2.
- 2. The isolated nucleic acid molecule of claim 1, wherein the nucleotide sequence comprises:
a) a nucleotide sequence of SEQ ID NO:1, fragment, domain or feature thereof; b) a nucleotide sequence having substantial similarity to (a); c) a nucleotide sequence capable of selectively hybridizing at high stringency to (a); d) a nucleotide sequence complementary to (a), (b) or (c); e) a nucleotide sequence which is the reverse complement of (a), (b) or (c).
- 3. An expression cassette comprising a promoter and the nucleic acid molecule of claim 1.
- 4. A recombinant vector comprising the expression cassette of claim 3.
- 5. A cell comprising the expression cassette of claim 3.
- 6. A transgenic plant comprising the expression cassette of claim 3.
- 7. Progeny and seed from the transgenic plant of claim 6.
- 8. The transgenic plant of claim 6, wherein the expression cassette is expressed in the tissue of the epidermis, vascular tissue, meristem, cambium, cortex, pith, leaf, sheath, root, flower, developing ovule or seed.
- 9. The transgenic plant of claim 6, wherein the plant is selected from the group consisting of: rice, wheat, barley, rye, corn, potato, canola, soybean, sunflower, carrot, sweet potato, sugarbeet, bean, pea, chicory, lettuce, cabbage, cauliflower, broccoli, turnip, radish, spinach, asparagus, onion, garlic, eggplant, pepper, celery, squash, pumpkin, cucumber, apple, pear, quince, melon, plum, cherry, peach, nectarine, apricot, strawberry, grape, raspberry, blackberry, pineapple, avocado, papaya, mango, banana, soybean, tobacco, tomato, sorghum and sugarcane.
- 10. The transgenic plant of claim 9, wherein the plant is rice.
- 11. The transgenic plant of claim 6, wherein the plant is a monocot.
- 12. The transgenic plant of claim 11, wherein the monocot is selected from the group consisting ofL maize, wheat, barley, oats, rye, millet, sorghum, trticale, secale, einkorn, spelt, emmer, teff, milo, flax, gramma grass, Tripsacum, and teosinte.
- 13. A transgenic plant comprising the nucleic acid molecule of claim 2.
- 14. Progeny and seed from the transgenic plant of claim 13.
- 15. The vector pNOV6605 having the Accession No. NRRL B-30635.
- 16. A method of enhancing pathogen or disease resistance in a plant, comprising expressing an expression cassette comprising a RAR1 encoding nucleic acid molecule or the expression cassette of claim 3 in the plant.
- 17. The method of claim 16, wherein the pathogen or disease is a nematode, bacteria, fungus, virus or viroid.
- 18. The method of claim 17, wherein the disease is selected from the group consisting of: Xanthomonas spp., Psudomonas spp., Rhizoctonia spp., Magnaporthe spp., Pythium spp., Phytophthora spp., Fusarium spp. Sclerotinia spp.
- 19. A plant produced by the method of claim 16, wherein the plant has enhanced pathogen or disease resistance.
- 20. A method of increasing expression of R disease resistance genes in a plant, comprising the step of expressing an expression cassette comprising a RAR1 encoding nucleic acid molecule or the expression cassette of claim 3 in the plant.
- 21. A method of increasing the expression of a coding sequence of interest comprising the steps of: expressing an expression cassette comprising an RAR1-regulated promoter and a coding region of interest in the transgenic plant of claim 6.
- 22. An isolated nucleic acid molecule comprising the sequence of SEQ ID NO:3 or 4.
- 23. A method of isolating a RAR1 homologue involved R gene expression leading to disease resistance in plants comprising the step of amplifying a nucleic acid molecule from a plant DNA library using the polymerase chain reaction with a pair of primers corresponding to the first 20 nucleotides of SEQ ID NO:1 and the reverse complement of the last 20 nucleotides of SEQ ID NO:1 or using at least one isolated nucleic acid molecule of claim 22.
- 24. The isolated nucleic acid molecule of claim 20, wherein the molecule encodes a polypeptide that enhances disease resistance when expressed in a plant.
- 25. A polypeptide comprising:
a) a polypeptide sequence of SEQ ID NO:2; b) a polypeptide sequence having substantial similarity to (a); c) a polypeptide sequence encoded by a nucleotide sequence identical or substantially similar to a nucleotide sequence of SEQ ID NO:1; d) a polypeptide sequence encoded by a nucleic acid molecule capable of hybridizing under high stringency conditions to a nucleic acid molecule listed in SEQ ID NO: 1 or to a sequence complementary thereto; and e) a functional fragment of (a), (b), (c) or (d).
- 26. A method of producing a polypeptide of claim 25, comprising the steps of:
a) growing recombinant cells comprising an expression cassette under suitable growth conditions, the expression cassette comprising a nucleic acid molecule of claim 1; and b) isolating the polypeptide from the recombinant cells.
- 27. An antibody cross-reactive to the polypeptide of claim 25.
- 28. A method of decreasing the expression of a RAR1 homologue in a plant comprising:
(a) expressing in said plant a DNA molecule of claim 1 or a portion thereof in “sense” orientation; or (b) expressing in said plant a DNA molecule of claim 1 or a portion thereof in “anti-sense” orientation; or (c) expressing in said plant a ribozyme capable of specifically cleaving a messenger RNA transcript encoded by an endogenous gene corresponding to a DNA molecule of claim 1; or (d) expressing in a plant an aptamer specifically directed to a protein encoded by a DNA molecules of claim 1; or (e) expressing in a plant a mutated or a truncated form of a DNA molecule of claim 1;(f) modifying by homologous recombination in a plant at least one chromosomal copy of the gene corresponding to a DNA molecule of claim 1; or g) modifying by homologous recombination in a plant at least one chromosomal copy of the regulatory elements of a gene corresponding to any one of the DNA molecules of claim 1; or h) expressing in said plant a DNA molecule of claim 1 or a portion thereof in the “sense” and “antisense” orientation.
- 29. A plant made by the method of claim 28, wherein the plant has decreased RAR1 expression compared to a parental plant.
- 30. The plant of claim 29, wherein the plant has decreased disease resistance.
Parent Case Info
[0001] This application claims the benefit of U.S. Provisional Patent Application No. 60/334,348 filed Nov. 30, 2001, which is hereby incorporated by reference in its entirety.
Provisional Applications (1)
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Number |
Date |
Country |
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60334348 |
Nov 2001 |
US |