Claims
- 1. An isolated nucleic acid molecule encoding a protein or polypeptide which controls papaya fruit ripening, wherein the protein or polypeptide is a β-galactosidase, and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 1, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 2, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 1 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 2. An isolated nucleic acid molecule according to claim 1, wherein the nucleic acid has a nucleotide sequence of SEQ ID NO: 1.
- 3. An isolated nucleic acid molecule according to claim 1, wherein the nucleic acid encodes a protein having an amino acid sequence of SEQ ID NO: 2.
- 4. An isolated nucleic acid molecule according to claim 1, wherein the nucleic acid hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 1 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 5. An isolated nucleic acid molecule encoding a protein or polypeptide which controls papaya fruit ripening, wherein the protein or polypeptide is a β-galactosidase, and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 3, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 4, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 3 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 6. An isolated nucleic acid molecule according to claim 5, wherein the nucleic acid has a nucleotide sequence of SEQ ID NO: 3.
- 7. An isolated nucleic acid molecule according to claim 5, wherein the nucleic acid encodes a protein having an amino acid sequence of SEQ ID NO: 4.
- 8. An isolated nucleic acid molecule according to claim 5, wherein the nucleic acid hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 3 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 9. A DNA construct comprising:
one or more operatively linked nucleic acid molecules, wherein the nucleic acid molecule encodes a protein or polypeptide which controls papaya fruit ripening; an operably linked heterologous DNA promoter, and an operably linked 3′ regulatory region.
- 10. The DNA construct according to claim 9, wherein the nucleic acid molecule which encodes a protein or polypeptide which controls papaya fruit ripening is selected from a group consisting of a β-galactosidase, a pectinmethylesterase, a polygalacturonase, and combinations thereof.
- 11. The DNA construct according to claim 9, wherein one or more of the nucleic acid molecules are in the sense (5′→3′) orientation.
- 12. The DNA construct according to claim 9, wherein one or more of the nucleic acid molecules are inserted in the antisense (3′→5′) orientation.
- 13. The DNA construct according to claim 9, wherein the nucleic acid molecule encodes a nontranslatable RNA.
- 14. A DNA construct according to claim 9, wherein the nucleic acid molecule encodes an antisense RNA.
- 15. The DNA construct according to claim 9, where the nucleic acid encodes a β-galactosidase, and the nucleic acid either: (a) has a nucleotide sequence of SEQ ID NO: 1, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 2, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 1 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 16. A DNA construct according to claim 9, wherein the nucleic acid molecule encodes a β-galactosidase and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 3, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 4, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 3 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 17. A DNA construct according to claim 9, wherein the nucleic acid molecule encodes a 62 -galactosidase and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 5, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 6, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 5 under stringent conditions characterized by a hybridization buffer comprising 533 SSC buffer at a temperature of 45° C.
- 18. A DNA construct according to claim 9, wherein the nucleic acid molecule encodes a pectinmethylesterase and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 7, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 8, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 7 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 19. A DNA construct according to claim 9, wherein the nucleic acid molecule encodes a polygalacturonase and the nucleic acid molecule either: (a) has a nucleotide sequence of SEQ ID NO: 9, (b) encodes a protein having an amino acid sequence of SEQ ID NO: 10, or (c) hybridizes to at least 20 nucleotides of the DNA molecule having a nucleotide sequence of SEQ ID NO: 9 under stringent conditions characterized by a hybridization buffer comprising 5×SSC buffer at a temperature of 45° C.
- 20. An expression system comprising:
the DNA construct according to claim 9.
- 21. A host cell transduced with the DNA construct according to claim 9.
- 22. A host cell according to claim 21, wherein the cell is selected from the group consisting of a bacterial cell, a virus, a yeast cell, and a plant cell.
- 23. A transgenic plant transformed with a DNA construct according to claim 9.
- 24. A transgenic plant according to claim 23, wherein the plant is papaya.
- 25. A transgenic plant transformed with a DNA construct according to claim 11.
- 26. A transgenic plant according to claim 25, wherein the plant is papaya.
- 27. A transgenic plant transformed with a DNA construct according to claim 12.
- 28. A transgenic plant according to claim 27, wherein the plant is papaya.
- 29. A transgenic plant transformed with a DNA construct according to claim 13.
- 30. A transgenic plant according to claim 29, wherein the plant is papaya.
- 31. A transgenic plant transformed with a DNA construct according to claim 14.
- 32. A transgenic plant according to claim 31, wherein the plant is papaya.
- 33. A method for promoting the ripening of papaya fruit, said method comprising:
transforming a papaya plant cell with the nucleic acid construct according to claim 11 and regenerating a papaya plant from the transformed cell under conditions effective to promote papaya fruit ripening.
- 34. A method for delaying the ripening of papaya fruit, said method comprising:
transforming a papaya plant cell with the DNA construct according to claim 12 and regenerating a papaya plant from the transformed cell under conditions effective to delay papaya fruit ripening.
- 35. A method for delaying the ripening of papaya fruit, said method comprising:
transforming a papaya plant cell with the DNA construct according to claim 13 and regenerating a papaya plant from the transformed cell under conditions effective to delay papaya fruit ripening.
Parent Case Info
[0001] The present invention claims benefit of U.S. Provisional Patent Application Serial No. 60/283,008, filed Apr. 11, 2001, and which is hereby incorporated by reference in its entirety.
Provisional Applications (1)
|
Number |
Date |
Country |
|
60283008 |
Apr 2001 |
US |