Nucleic acids and polypeptides specific of the neisseria genus pathogenic strains

The present invention relates to nucleic acids encoding polypeptides specific for pathogenic strains of the

Neisseria

genus, in particular which are useful for preventing or treating a

Neisseria meningitidis

infection.

In general, meningitis is either of viral origin or of bacterial origin. The bacteria mainly responsible are: type b

Haemophilus influenzae, Neisseria meningitidis

and

Streptococcus pneumoniae

. The

Neisseria meningitidis

species is subdivided into serogroups according to the nature of the capsular polysaccharides. Although about a dozen serogroups exist, 90% of meningitis cases can be attributed to three serogroups: A, B and C.

Effective vaccines based on capsular polysaccharides exist for preventing meningitis caused by

Neisseria meningitidis

serogroups A and C. These polysaccharides, unmodified, are only slightly immunogenic, or not at all, in children under the age of two, and do not induce any immune memory. However, these drawbacks can be overcome by conjugating these polysaccharides to a carrier protein.

On the other hand, the polysaccharide of

Neisseria meningitidis

serogroup B is non-immunogenic, or relatively non-immunogenic in humans, whether or not it is in a conjugated form. Thus, it appears to be highly desirable to seek a vaccine against meningitis caused by

Neisseria meningitidis

, in particular

Neisseria meningitidis

serogroup B, other than a vaccine based on polysaccharide.

To this end, various proteins of the external membrane of

N. meningitidis

have already been proposed, such as the membrane-bound receptor for human transferrin (WO 90/12591 and WO 93/06861).

Neisseria meningitidis

is genetically very close to

Neisseria gonorrhoeae

and

Neisseria lactamica. N. gonorrhoeae

is especially responsible for infections located in the urogenital tract. It colonizes the genital mucous membrane, crosses the epithelium and then invades the sub-epithelium, where it multiplies and is responsible for a severe inflammatory reaction. On the other hand,

N. lactamica

is considered to be a nonpathogenic species.

Sequences present in

N. gonorrhoeae

and

N. meningitidis

, but absent from

N. lactamica

, have been disclosed in patent application WO 98/02547, but this prior patent application does not locate or identify the coding sequences.

The authors of the present invention have now managed to identify some of these genes by searching, in the meningococcal genome, for the open reading frames specific for pathogenic strains of the

Neisseria

genus, using the following strategy:

Some of the sequences disclosed in patent application WO 98/02547 (referred to, in said prior application, as SEQ ID Nos 66, 67, 69, 70, 72 to 96, 98 and 99) were positioned on the sequence of the genome of the

N. meningitidis

serogroup B strain (ATCC 13090), available from the Pathoseq® bank of Incyte Pharmaceuticals, and also on the sequence of the genome of the

Neisseria meningitidis

strain Z2491 (Sanger Centre). This made it possible to identify, in the

N. meningitidis

genome which has 2.3 mega bases, 19 contigs representing 220000 base pairs.

The authors of the present invention then analysed, for each of the 19 contigs, the presence of open reading frames (ORFs) containing at least 100 amino acids (and, by definition, bordered by an initiation codon and a stop codon), using the Gene Jockey II sequence processor® program (Biosoft). This analysis made it possible to select approximately 400 candidate ORFs.

The sequences of each of these ORFs were then analysed using the Codon Use® program (Conrad Halling), which takes into account the codon use frequency in

N. meningitidis

. Only the ORFs with sequences having a maximum frequency of use of these codons were selected. At the end of this analysis, 197 candidate ORFs were selected.

The ORFs selected using this double analysis were subjected to a homology search through all of the available banks, using the BLASTX® program, from the access to the Pathoseq® bank of Incyte Pharmaceuticals. This homology search made it possible to exclude the ORFs encoding, a priori, cytoplasmic or periplasmic proteins, in particular metabolism proteins. The ORFs were also subjected to analysis of possible protein motifs, using the DNA Star Protean® program (Lasergene software).

The authors of the present invention then investigated whether the ORFs selected at the end of the previous step (118 in number) were effectively absent from

N. lactamica

, as predicted by the application of the prior art WO 98/02547.

To this end, a PCR amplification was carried out. This amplification proved to be ineffective for 78 of the 118 ORFs tested. Only the ORFs for which the amplification in

N. lactamica

was negative (sequences named “lactamical

−

”) were selected. In order to verify that these negative results were not “false negatives”, the lactamica

−

sequences selected were subjected to a control by dot blot. At the end of this step, only 23 ORFs were confirmed

N. meningitidis

+

/N. lactamica

−

.

Finally, these 23 ORFs were repositioned in their entirety on the

N. meningitides

ATCC13090 genome. This made it possible to demonstrate that three ORFs previously eliminated on the basis of their putative protein function appeared to be located close to, or were even framed by, some of the 23

N. meningitidis

+

/N. lactamica

−

ORFs. These three ORFs (SEQ ID Nos 29, 35 and 37) were reintroduced into the study, and it was proven that they were also

N. meningitidis

+

/N. lactamica

−

.

The authors of the present invention then attempted to discover whether the ORFs identified using the genome of the

N. meningitidis

serogroup B strain ATCC 13090 were also present in the genomes of

N. meningitidis

serogroup A Z2491 (Sanger Centre) and of

N. gonorrhoeae

FA1090 (Advanced Centre of Genome Technology, Oklahoma University). Then, they compared the sequences derived from these various genomes, with multiple alignment (Clustal, Infobiogen). This made it possible to redefine, for some of the ORFs, the most probable position of the initiation codon and translation stop codon. The sequences of the open reading frames derived from the strain ATCC13090 are given in the SEQ ID Nos 1-51 (odd numbers) and the amino acid sequences which are deduced therefrom are given in the SEQ ID Nos 2-52 (even numbers).

A subject of the present invention is, therefore, a nucleic acid in isolated form encoding a polypeptide, or an antigenic fragment thereof, excluding the nucleic acids disclosed in SEQ ID Nos 70, 73, 74, 77, 80, 81, 87, 88, 89, 94, 95 and 98 of application WO 98/02547 (sequences attached to the present description and numbered SEQ ID Nos 70A, 73A, 74A, 77A, 80A, 81A, 87A, 88A, 89A, 94A, 95A and 98A so as to distinguish them from the sequences of the invention); said polypeptide having an amino acid sequence which is identical or homologous to a sequence selected from those of group II; group II consisting of the sequences shown in SEQ ID Nos 2-52 (even numbers) and the sequence SEQ ID No. 53.

Preferably, said nucleic acid can have a nucleotide sequence selected from those of group I, group I consisting of the sequences shown in SEQ ID Nos 1-51 (odd numbers).

The term “nucleic acid” includes and means equally ORF, gene, polynucleotide, DNA and RNA. The term “nucleic acid in isolated form” means a nucleic acid separated from the biological environment in which it is found under natural conditions. For example, a DNA molecule exists under natural conditions when it is integrated into a genome or when it forms part of a library of genes. In that case, it cannot be in isolated form. On the other hand, the same molecule separated from the genome by cloning (for example subsequent to a PCR amplification) should be considered as being in isolated form. Typically, a DNA molecule in isolated form does not contain the coding regions which are contiguous with it in 5′ and 3′ in the genome from which it is derived. The nucleic acids in isolated form can be integrated into vectors (for example plasmids, or viral or bacterial vectors) without, even so, abandoning their characteristic of being separated from their natural environment.

The authors of the present invention have more particularly found that the ORFs which, when they are derived from the strain ATCC 13090, are characterized by the sequences as shown in SEQ ID Nos 19, 27, 39, 45, 47 and 49 are specific for

Neisseria meningitidis

insofar as it has not been possible to demonstrate identical or homologous sequences in the

N. gonorrhoeae

genome. They have also found that the ORF characterized by the strain sequence as shown in SEQ ID No. 39 is specific for

Neisseria meningitidis

serogroup B.

A subject of the invention is also a polypeptide in isolated form, or a fragment thereof; said polypeptide having an amino acid sequence identical or homologous to a sequence selected from those of group II.

The amino acids framed in the sequence SEQ ID No. 8 correspond to the signal sequence, and the amino acid in bold represents the first amino acid of the mature form. The amino acid sequence of the mature protein form is represented in SEQ ID No. 53.

In the context of the present invention, the terms “polypeptide” and “protein” are equivalent and mutually interchangeable. They refer to any amino acid chain, whatever its length and its post-translational modifications (for example phosphorylation or glycosylation).

The expression “antigenic fragments of the polypeptides specific for pathogenic strains of the

Neisseria

genus” is intended to mean the polypeptides derived from the polypeptides of the invention as defined above, through deletions of portions of said polypeptides without destroying the antigenicity (for example, without notable loss of the antigenic activity) of said polypeptides. The specific antigenicity can be determined using various methods known to those skilled in the art, as explained later.

These fragments are preferably at least 12 amino acids long, more preferably at least 20 amino acids long, preferentially 50 amino acids long, more preferably still 75 amino acids long, preferentially 100 amino acids long.

These fragments can be used to reveal epitopes which may be masked in the parent polypeptides. They are also advantageous for inducing a T-lymphocyte-dependent protective immune response. The deletions can, in fact, make it possible to eliminate immunodominant regions which are highly variable between various strains.

Such fragments can be obtained using standard techniques known to those skilled in the art (for example, Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons Inc, 1994), for example by PCR, RT-PCR or treatment with restriction enzymes for the cloned DNA molecules, or by the method of Kunkel et al. (Proc. Natl. Acad. Sci. USA (1985) 82:448).

The expression “homologous amino acid sequence” is intended to mean a sequence which differs from one of the sequences of group II by substitution, deletion and/or insertion of one or more amino acids, at positions such that these modifications do not destroy the specific antigenicity of the polypeptide in question.

Said substitutions are preferably conservative substitutions, i.e. substitutions of amino acids of the same class, such as substitutions of amino acids with uncharged side chains (for instance asparagine, glutamine, serine, threonine and tyrosine), of amino acids with basic side chains (for instance lysine, arginine and histidine), of amino acids with acid side chains (for instance aspartic acid and glutamic acid) or of amino acids with apolar side chains (for instance glycine, alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan and cysteine).

Advantageously, a homologous amino acid sequence has at least a 75% degree of homology (i.e. of identity) with one of the sequences of group II; preferably this degree of homology is at least 80%, most preferably at least 90%. The homologous amino acid sequences include, in particular, the sequences which are substantially identical to one of the sequences of group II. The expression “substantially identical sequence” means a sequence which has at least a 90%, advantageously at least a 95%, preferably at least a 97%, and most preferably at least a 99%, degree of homology (i.e. of identity) with one of the sequences of group II. In addition, it may differ from the reference sequence only through mainly conservative substitutions.

The degree of homology (also named degree of identity) is generally determined using a sequence analysis program (for example, Sequence Analysis Software Package of the Genetics Computer Group, University of Wisconsin Biotechnology Centre, 1710 University Avenue, Madison, Wis. 53705). Similar amino acid sequences are aligned so as to obtain the maximum degree of homology (i.e. identity). To this end, it may be necessary to artificially introduce gaps into the sequence. Once optimal alignment has been produced, the degree of homology (i.e. identity) is established by recording all the positions for which the amino acids of the two sequences compared are identical, with respect to the total number of positions.

The expression “homologous nucleotide sequences” is intended to mean sequences which differ from the sequences of group I by substitution of one or more nucleotides, or by deletion and/or insertion of one or more codons, at positions such that these sequences (i) still encode polypeptides having the sequences of group II, due to the effect of the degeneracy of the genetic code; or (ii) encode polypeptides having homologous sequences as defined above.

Advantageously, a homologous nucleotide sequence has at least a 60% degree of homology with one of the sequences of group I; preferably this degree of homology is at least 80%, most preferably at least 90%.

Typically, a homologous nucleotide sequence hybridizes specifically to the sequences complementary to the sequences of group I, under stringent conditions. The temperature at which the hybridization assay is carried out constitutes an important factor which influences the stringency. Conventionally, this temperature, termed hybridization temperature (Th), is selected from 5 to 40° C., preferably from 20 to 25°

0

C., below the temperature at which 50% of the paired strands separate (Tm). In general, it is considered that conditions of high stringency are satisfied when Th is lower than Tm by 5 to 25° C. approximately, for example by 5 to 10° C. or, most commonly, by 20 to 25° C. approximately. Moderate stringency is established when Th is lower than Tm by 30 to 40° C.

For sequences comprising more than 30 bases, the temperature Tm is defined by the equation: Tm=81.5+0.41(%G+C)+16.6Log(cation concentration)−0.63(%formamide)−(600/number of bases). Thus, ionic strength has a major impact on the value of Tm. The temperature Tm increases by 16.60° C. every time the monovalent cation concentration increases by a factor of 10. The addition of formamide into the hybridization buffer causes, on the other hand, the value of Tm to decrease. (For a complete reference, see Sambrook et al., Molecular Cloning, A laboratory manual, Cold Spring Harbor Laboratory Press, 1989, pages 9.54-9.62).

Conventionally, hybridization experiments are carried out at a temperature of 60 to 68° C., for example at 65° C. At this temperature, stringent hybridization conditions can, for example, be implemented in 6×SSC, advantageously in 2×SSC or 1×SSC, preferably in 0.5×SSC, 0.3×SSC or 0.1×SSC (in the absence of formamide). A solution of 1×SSC contains 0.15 M of NaCl and 0.015 M of sodium citrate.

For this reason, in other words, a subject of the invention is a polynucleotide in isolated form, which is capable of hybridizing, under stringent conditions, with a DNA molecule having one of the nucleotide sequences as shown in SEQ ID Nos 1-51 (odd numbers) or the sequences complementary thereto.

A specific class of homologous sequences consists of those encountered naturally by virtue of the extremely common phenomenon of allelic variation. A bacterial species, for example

N. meningitidis

or

N. gonorrhoeae

, consists of a large variety of strains which differ from one another through minor variations, termed allelic variations. Thus, a polypeptide which is present in various strains and which, of course, performs the same biological function in each of them, can have an amino acid sequence which is not identical from one strain to the other. In other words, the sequences derived from the allelic variation are purely sequences equivalent or alternative to those of group II. The class of sequences which are allelic variants of one of the sequences of group II consists of the sequences of the polypeptide as found in a pathogenic species of the

Neisseria

genus (for example,

N. meningitidis

or

N. gonorrhoeae

) other than the

N. meningitidis

strain ATCC 13090. The biological function which is associated with the allelic variant sequences is the same as that which is associated with the reference sequence. The differences (substitution, deletion or addition of one or more amino acids) which they exhibit between one another (including the reference sequence) do not modify the biological function of the polypeptide. The term “biological function” is intended to mean the function exercised by the polypeptide in the cells which produce it naturally.

The allelic variation is also expressed in the coding sequences. A polynucleotide, encoding a polypeptide, having a sequence which is an allelic variant of one of the sequences of group I can be easily cloned by amplifying the genomic DNA of the strains of pathogenic species of the

Neisseria

genus, for example by PCR (polymerase chain reaction), using synthetic oligonucleotide primers capable of hybridizing to the 5′ and 3′ ends of the coding region. The sequences of such primers can easily be established by those skilled in the art using the nucleotide sequences given in SEQ ID Nos 1-51 (odd numbers). The primers generally have from 10 to 40 nucleotides, preferably from 15 to 25 nucleotides.

For this reason, in other words, a subject of the invention is a DNA molecule in isolated form which can be amplified and/or cloned by PCR from the genome of a pathogenic

Neisseria

strain, using a pair of 5′ and 3′ PCR primers; the sequences of these primers being established using one of the nucleotide sequences as shown in SEQ ID Nos 1-51 (odd numbers). An example is given, for each pair of primers, in Example I.1 hereinafter.

A subject of the present invention is more particularly the allelic variants having the nucleotide sequences SEQ ID Nos 54 to 76 (even numbers) and the products encoded by these nucleotide sequences, having the amino acid sequences SEQ ID Nos 55 to 77 (odd numbers).

The polypeptides of the invention can be fused to other polypeptides, for example by translation of a hybrid gene. Vectors for expressing fusion polypeptides are commercially available, such as the vectors pMal-c2 or pMal-p2 from New England Biolabs, in which the protein to which the polypeptides of the invention can be fused is a maltose-binding protein, the glutathione-S-transferase system from Pharmacia or the His-Tag system from Novagen. Such systems are in particular useful for purifying the polypeptides of the invention. The polypeptides of the invention can be fused to polypeptides having adjuvant activity, such as for example the B subunit of cholera toxin or the B subunit of the

E. coli

heat-sensitive toxin.

The nucleic acids of the present invention can be used (i) in a process for producing the polypeptides encoded by said nucleic acids, in a recombinant host system, (ii) for the construction of vaccination vectors, such as poxviruses, intended to be used in methods and compositions for preventing and/or for treating an infection with pathogenic

Neisseria

strains, in particular with

Neisseria meningitidis

, (iii) as a vaccination agent in a naked form or in combination with a vehicle which promotes transfer to the target cells and, (iv) in the construction of attenuated

Neisseria

strains which can overexpress a nucleic acid of the invention, or express it in a non-toxic, mutated form.

The present invention also provides (i) an expression cassette containing a polynucleotide of the invention placed under the control of elements allowing its expression, in particular under the control of a suitable promoter; (ii) an expression vector containing said expression cassette; (iii) a host cell (prokaryotic or eukaryotic) transformed with an expression cassette and/or an expression vector as defined above, and (iv) a method for obtaining a polypeptide encoded by said polynucleotide of the invention, comprising culturing said transformed cell under conditions allowing the expression of the polynucleotide of the invention, and recovering the polypeptide from the cell culture.

Among the eukaryotic hosts which can be used, mention may be made in particular of yeast cells (for example

Saccharomyces cerevisiae

or

Pichia Pastoris

), mammalian cells (for example COS1, NIH3T3 or JEG3) arthropod cells (for example

Spodoptera frugiperda

(SF9)) and plant cells. Among the prokaryotic hosts which can be used, mention may be made in particular of

E. coli.

The choice of the expression cassette depends on the host system chosen, and also on the characteristics desired for the expressed polypeptide. In general, expression cassettes include a promoter which is functional in the host system selected and which can be constitutive or inducible; a ribosome-binding site; an initiation codon (ATG); if necessary, a region encoding a signal peptide; a nucleotide sequence of the invention; a stop codon; and, optionally, a 3′ terminal region (translation and/or transcription terminator). The open reading frame (ORF) consisting of the nucleotide sequence of the invention, alone or associated with the region encoding the signal peptide, is placed under the control of the promoter such that translation and transcription take place in the host system. The promoters and regions encoding the signal peptides are known to those skilled in the art. Among them, mention may be made in particular of the arabinose-inducible promoter (araB promoter) of

Salmonella typhimurium

, which is functional in Gram

−

bacteria such as

E. coli

(U.S. Pat. No. 5,028,530 and Cagnon et al., Protein Engineering (1991) 4(7): 843), the promoter of the T7 bacteriophage gene encoding RNA polymerase (U.S. Pat. No. 4,952,496), and the OspA and RlpB signal peptide (Takase et al., J. Bact. (1987) 169:5692).

The polypeptide expressed can be recovered in a practically purified form from the cell extract or from the supernatant, after centrifuging the recombinant cell culture. The recombinant polypeptide can, in particular, be purified using methods of affinity purification with the aid of antibodies, or using any other method known to those skilled in the art, for instance by genetic fusion with a small binding domain.

The nucleic acids of the invention can also be used in the field of vaccination, either by using a viral or bacterial host as a vehicle for releasing the DNA, or by administering the nucleic acid of interest in a free form.

A subject of the present invention is also (i) a vaccination vector containing a nucleic acid of the invention, placed under the control of elements allowing its expression; (ii) a pharmaceutical composition containing a therapeutically or prophylactically effective amount of said vaccination vector; (iii) a method for inducing an immune response against

Neisseria

in a vertebrate, in particular a mammal, preferably a human, said method comprising the administration to said vertebrate of an immunologically effective amount of said vaccination vector so as to cause an immune response, in particular a protective or therapeutic response to

Neisseria meningitidis

; and (iv) a method for preventing and/or treating an infection with pathogenic

Neisseria

strains, in particular with

Neisseria meningitidis

, which comprises the administration of a prophylactic or therapeutic amount of said vaccination vector of the invention to an individual requiring such a treatment.

In combination with the polypeptides of the invention, the vaccination vector as defined above can also comprise nucleotide sequences the expression of which allows the immune response to be stimulated, such as the sequences encoding cytokines.

Said vaccination vector of the invention can be administered via any route which is conventional in the field of vaccination, in particular via the parenteral route (for example subcutaneous, intradermal, intramuscular, intravenous or intraperitoneal route). The dose depends on many parameters which are known to those skilled in the art, such as the vector itself, the route of administration, or the weight, age or sex of the animal or of the human to be vaccinated.

A subject of the present invention is also (i) a pharmaceutical composition containing a therapeutically or prophylactically effective amount of a polynucleotide of the invention; (ii) a method for inducing an immune response against pathogenic

Neisseria

strains, in particular

Neisseria meningitidis

in a vertebrate, by administering to said vertebrate an immunologically effective amount of said polynucleotide so as to cause an immune response, in particular a protective immune response against pathogenic

Neisseria

strains, especially

Neisseria meningitidis

; and (iii) a method for preventing and for treating an infection with pathogenic

Neisseria

strains, in particular with

Neisseria meningitidis

, by administering a therapeutic or prophylactic amount of said polynucleotide to an individual requiring such a treatment.

The polynucleotides of the invention (DNA or RNA) can be administered to a vertebrate as they are. When a DNA molecule of the invention is used, it can be in the form of a plasmid incapable of replicating in a vertebrate cell and incapable of integrating the genome of said vertebrate. Said DNA molecule is, typically, placed under the control of a promoter suitable for expression in a vertebrate cell. Said polynucleotide used as vaccine can be formulated according to various methods known to those skilled in the art. Said polynucleotide can, in particular, be used in a naked form, free of any vehicle which promotes transfer to the target cell, such as anionic liposomes, cationic lipids, microparticles, for example gold microparticles, precipitation agents, for example calcium phosphate, or any other agent which facilitates transfection. In this case, the polynucleotide can be simply diluted in a physiologically acceptable solution, such as a sterile solution or a sterile buffer solution, in the presence or absence of a vehicle. When it is present, this vehicle can be preferably isotonic, hypotonic or slightly hypertonic, and has a relatively low ionic strength. It can, for example, be a sucrose solution (for example a solution containing 20% of sucrose).

Alternatively, a polynucleotide of the invention can be combined with agents which facilitate transfection. It can be, inter alia, (i) combined with a chemical agent which modifies cell permeability, such as bupivacain (WO 94/16737); (ii) encapsulated in liposomes, optionally in the presence of additional substances which facilitate transfection (WO 93/18759, WO 93/19768, WO 94/25608 and WO 95/2397, WO 93/18759 and WO 93/19768); or (iii) combined with cationic lipids, or silica, gold or tungsten microparticles.

When the polynucleotides of the invention coat microparticles, these particles can be injected via the intradermal or intraepidermal route, using the “gene gun” technique (U.S. Pat. Nos. 4,945,050, 5,015,580 and WO 94/24263).

The amount of DNA to be used as a vaccine depends, in particular, on the strength of the promoter used in the DNA construct, on the immunogenicity of the product expressed, on the individual to which this DNA is administered, on the method of administration and on the type of formulation. In general, a therapeutically or prophylactically effective amount ranging from approximately 1 μg to approximately 1 mg, preferably from approximately 10 μg to approximately 800 μg, and preferentially from approximately 25 μg to approximately 250 μg, can be administered to human adults.

The polynucleotide of the invention can be administered via any conventional route of administration, such as in particular via the parenteral route. The choice of the route of administration depends, in particular, on the formulation chosen. A polynucleotide formulated in combination with bupivacain is advantageously administered into muscle. When neutral or anionic liposomes, or a cationic lipid such as DOTMA (N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride) or DC-Chol (3-beta-(N-(N′,N′-dimethyl-aminomethane)carbamoyl)cholesterol) are used, the formulation can advantageously be injected via the intravenous, intramuscular, intradermal or subcutaneous route. A polynucleotide in a naked form can advantageously be administered via the intramuscular, intradermal or subcutaneous route.

The nucleotide sequences of the invention allow the construction of specific nucleotide probes and primers which can be used in diagnosis. Said probes or primers are nucleic acids having sequences identical or homologous to portions of the sequences of group I or to the sequences complementary thereto.

Preferably, said probes contain from approximately 5 to approximately 100, preferably from approximately 10 to approximately 80, nucleotides. They can contain modified bases, the sugar and phosphate residues possibly also being modified or substituted. The probes of the invention can be used in diagnostic tests, to capture or detect polynucleotides specific for pathogenic

Neisseria

strains. Such capture probes can conventionally be immobilized on a solid support directly or indirectly, by covalent bonding or by passive adsorption. A detection probe can be labelled, in particular with a radioactive isotope, an enzyme such as peroxidase or alkaline phosphatase, or enzymes capable of hydrolyzing a chromogenic, fluorogenic or luminescent substrate, or with compounds which are, themselves, chromogenic, fluorogenic or luminescent, nucleotide analogues; or biotin.

A primer generally contains from approximately 10 to approximately 40 nucleotides, and can be used to initiate enzymatic polymerization of the DNA in an amplification process (for example PCR), in an elongation process or in a reverse transcription method. A primer of the invention can in particular be a primer as described in Example II.1 hereinafter.

A subject of the present invention is also:

(i) a reagent containing a probe of the invention for detecting and/or identifying the presence of pathogenic

Neisseria

strains in a biological sample;

(ii) a process for detecting and/or for identifying the presence of pathogenic

Neisseria

strains in a biological sample, said method comprising the steps consisting in a) extracting the DNA or RNA from a biological sample and denaturing it; b) exposing said DNA or said RNA to a probe of the invention, under stringent hybridization conditions, so as to detect the hybridization; and

(iii) a method for detecting and/or for identifying pathogenic

Neisseria

strains in a biological sample, in which the DNA is extracted from a biological sample and mixed together with at least one and preferably with two primers of the invention, and is amplified, for example by PCR.

As mentioned above, the polypeptides produced by the expression of the ORF sequences identified can be used as vaccination agents. The specific antigenicity of the polypeptides homologous to the polypeptides having sequences of group II can be evaluated by assaying the cross-reactivity with an antiserum directed against the polypeptides having sequences of group II. A monospecific hyperimmune antiserum can be produced against a purified polypeptide having a sequence of group II or a fusion polypeptide, for example an expression product of the MBP, GST or His-tag systems.

The specific antigenicity can be determined using various methods known to those skilled in the art, in particular the Western blot, dot blot and ELISA techniques, described below.

In the Western blot technique, the protein preparation to be tested is subjected to SDS-PAGE gel electrophoresis. After transfer onto a nitrocellulose membrane, the material is incubated with a monospecific hyperimmune antiserum obtained after having immunized an animal with the referent material; i.e., in the present case, with a polypeptide having an amino acid sequence of group II. This antiserum is diluted beforehand in a dilution range of approximately 1:50 to 1:5000, preferably of approximately 1:100 to 1:500. The specific antigenicity is revealed when a band corresponding to the product shows reactivity with one of the dilutions above.

In the ELISA assay, a purified protein preparation is preferably used, although a whole cell extract may also be used. Approximately 100 μl of a preparation at approximately 10 μg/ml are distributed into the wells of a plate. The plate is incubated for two hours at 37° C., and then overnight at 4° C. The plate is then washed with a phosphate buffered saline solution (PBS) comprising 0.05% of Tween 20. The wells are saturated with 250 μl of PBS containing 1% of bovine serum albumin (BSA) so as to prevent non-specific antibody binding. After incubation for one hour at 37° C., the plate is washed with the PBS/Tween buffer. The antiserum is serially diluted in PBS/Tween buffer containing 0.5% BSA. 100 μl of this dilution are added per well. The plate is incubated for 90 minutes at 37° C., washed and evaluated according to standard procedures. For example, when specific antibodies are produced in rabbits, a goat anti-rabbit peroxidase conjugate is added to the wells. The incubation is carried out for 90 minutes at 37° C. and the plate is then washed. The reaction is measured by colorimetry (the reaction is positive when the optical density value is 1, if the dilution is at least 1:50, preferably at least 1:500).

In the dot blot assay, a purified protein is preferably used, it being understood that it is also possible to use a whole cell extract. Two-fold serial dilutions of a protein solution at approximately 100 μg/ml are prepared in a 50 mM Tris-HCl buffer, pH: 7.5. 100 μl of each dilution are applied to a nitrocellulose membrane (BioRad apparatus). The buffer is removed by applying suction to the system. The wells are washed by adding 50 mM of Tris-HCl buffer (pH: 7.5) and the membrane is air-dried. The membrane is then saturated in a blocking buffer (50 mM Tris-HCl (pH: 7.5) 0.15 M NaCl, 10 g/l of skimmed milk) and incubated with a dilution of antiserum ranging from approximately 1:50 to 1:5000, preferably to approximately 1:500. The reaction is revealed in accordance with standard procedures. For example, when specific antibodies are produced in rabbits, a goat anti-rabbit peroxidase conjugate is added to the wells. The incubation is carried out for 90 minutes at 37° C. The reaction is developed with the suitable substrate and measured, for example by colorimetry, by the appearance of a coloured spot (a reaction is positive when a coloured spot appears in association with a dilution of at least 1:50, preferably of at least 1:500).

A subject of the present invention is also (i) a pharmaceutical composition containing a therapeutically or prophylactically effective amount of a polypeptide of the invention; (ii) a method for inducing an immune response against pathogenic

Neisseria

strains in a vertebrate, by administering to said vertebrate an immunogenically effective amount of a polypeptide of the invention so as to cause an immune response, in particular a protective immune response against pathogenic

Neisseria

strains; and (iii) a method for preventing and/or for treating an infection with pathogenic

Neisseria

strains, by administering a therapeutic or prophylactic amount of a polypeptide of the invention to an individual requiring such a treatment.

The immunogenic compositions of the invention can be administered via any route which is conventional in the field of vaccination, in particular via the parenteral route (for example subcutaneous, intradermal, intramuscular, intravenous or intra-peritoneal route). The choice of the route of administration depends on a certain number of parameters, such as the adjuvant combined with the polypeptide.

A composition of the invention contains at least one polypeptide as defined above. It can also contain at least one additional antigen of

Neisseria meningitidis

and/or

Neisseria gonorrhoeae.

The polypeptides of the invention can be formulated with liposomes, preferably neutral or anionic liposomes, microspheres, ISCOMS or “virus-like” particles, in order to facilitate the transfer of the polypeptide and/or to increase the immune response.

The administration can be carried out with a single dose or with doses repeated, if necessary, at intervals which can be determined by those skilled in the art.

For example, an initial dose can be followed by three booster doses at intervals of one or more weeks or of one or more months. The suitable dose depends on many parameters, including the individual treated (adult or child), the specific vaccination antigen, the route of administration and the frequency of administration, the presence or absence or the type of adjuvant, and the desired effect (for example protection and/or treatment), and can be determined by those skilled in the art. If the route of administration is parenteral, the dose is preferentially less than 1 mg, preferably approximately 100 μg. The polypeptides and polynucleotides of the invention used as vaccination agents can be used sequentially, in a several-step immunization process. For example, a vertebrate can be initially sensitized with a vaccination vector of the invention, such as a poxvirus, for example via the parenteral route, and can then be stimulated twice with the polypeptide encoded by the vaccination vector.

A polypeptide of the invention can also be useful as a diagnostic agent for detecting the presence of anti-

Neisseria meningitidis

and/or anti-

Neisseria gonorrhoeae

antibodies in a biological sample such as a blood sample.

A subject of the present invention is also monospecific antibodies directed against the polypeptides of the invention.

The term “monospecific antibodies” is intended to mean an antibody capable of reacting specifically with a

Neisseria

polypeptide of the invention. Such antibodies can be polyclonal or monoclonal, and can be recombinant antibodies, for example chimeric (for example consisting of a variable region of murine origin associated with a constant region of human origin), humanized and/or single-chain antibodies. Said antibodies can also be in the form of immunoglobulin fragments, for example F(ab)′2 or Fab fragments. The antibodies of the invention can be of any isotype, for example IgA or IgG, the polyclonal antibodies possibly being of a single isotype or possibly containing a mixture of several isotypes.

The antibodies of the invention directed against the polypeptides of the invention can be produced and identified using standard immunological methods, for example Western blot analysis, a dot blot assay, an ELISA assay (Coligan et al., Current Protocols in Immunology (1994) John Wiley & Sons, Inc., New York, N.Y.). Said antibodies can be used in diagnostic processes for detecting the presence of a

Neisseria meningitidis

antigen in a sample such as, in particular, a biological sample (for example a blood sample).

The antibodies of the invention can also be used in affinity chromatography processes for purifying a polypeptide of the invention. Finally, such antibodies can also be used in prophylactic or therapeutic passive immunization methods.

A subject of the present invention is also a diagnostic method for detecting the presence of pathogenic

Neisseria

strains in a biological sample, comprising bringing said biological sample into contact with an antibody or a polypeptide of the invention, such that an immune complex is formed, and detecting said complex which indicates pathogenic

Neisseria

strains in the organism from which the sample originates. Those skilled in the art understand that the immune complex is formed between a component of the sample and the antibody or the polypeptide of the invention, any substance not bound possibly being eliminated prior to the detection of the complex.

Thus, a reagent of polypeptide type can be used for detecting the presence of anti-

Neisseria meningitidis

and/or

Neisseria gonorrhoeae

antibodies in a sample, whereas an antibody of the invention can be used as a reagent for assaying the presence of a

Neisseria meningitidis

and/or

Neisseria gonorrhoeae

polypeptide in a sample.

For use in diagnostic applications, the reagent (for example the antibody or the polypeptide of the invention) can be in the free state or immobilized on a solid support, by direct or indirect means.

The direct means include passive adsorption or covalent bonding between the support and the reagent.

The term “indirect means” is intended to mean that a substance which interacts with said reagent is attached to the solid support. For example, if a reagent of polypeptide type is used, an antibody which binds to this polypeptide can be used as an anti-reagent substance, it being understood that this substance binds to an antibody which is not involved in recognizing the antibodies in the biological samples.

Among the indirect means which can be used, mention may also be made of the ligand receptor system, a molecule such as a vitamin possibly being grafted onto the reagent of polypeptide type, and the corresponding receptor possibly being immobilized on the solid phase. This is illustrated by the biotin-streptavidin system. It is also possible to add a peptide tail to the reagent, by chemical engineering or genetic engineering, and to immobilize the grafted or fused product by passive adsorption or covalent bonding with the peptide tail.

A subject of the present invention is also a process for purifying, from a biological sample, a

Neisseria

polypeptide of the invention, by affinity chromatography with a monospecific antibody of the invention. Said antibody is preferably of isotype IgG.

According to an example of implementation; a biological sample, preferably in a buffer solution, is applied to a chromatographic material, preferably equilibrated with the buffer used to dilute the biological sample, such that the polypeptide of the invention (i.e. the antigen) may adsorb to the material. The unbound components are washed and the antigen is then eluted with a suitable elution buffer, such as a glycine buffer or a buffer containing chaotropic agent, for example guanidine HCl, or a high concentration of salt (for example 3 M MgCl

2

). The eluted fractions are recovered and the presence of antigen is detected, for example by measuring the absorbence at 280 nm.

A subject of the present invention is also (i) a pharmaceutical composition containing a therapeutically or prophylactically effective amount of a monospecific antibody of the invention; and (ii) a method for preventing and/or for treating an infection with pathogenic

Neisseria

strains, by administering a therapeutic or prophylactic amount of a monospecific antibody of the invention to an individual requiring such a treatment.

To this end, the monospecific antibody of the invention is preferably of isotope IgG, and preferably fixes the complement. Said monospecific antibody according to the invention can be administered alone or in a mixture with at least one other monospecific antibody, specific for a different

Neisseria meningitidis

and/or

Neisseria gonorrhoeae

polypeptide, according to the invention. The amount of antibody can be determined easily by those skilled in the art. For example, a daily administration of approximately 100 to 1000 mg of antibodies over a week, or three daily doses of approximately 100 to 1000 mg of antibodies over two or three days, may be an effective dose.

The therapeutic or prophylactic effectiveness may be evaluated using standard methods known to those skilled in the art, for example by measuring the induction of an immune response or the induction of protective and/or therapeutic immunity (in newborn rats or mice), through evaluation of the bacterial load in the cerebrospinal fluid. The protection can be determined by comparing the degree of

Neisseria

infection to a control group. Protection is demonstrated when the infection is decreased in comparison with the control group. Such an evaluation can be carried out with the polynucleotides, the vaccination vectors, the polypeptides and also the antibodies according to the invention. The therapeutic or prophylactic effectiveness of a product according to the invention (polynucleotide or polypeptide) can also be evaluated in an assay for bactericidal activity, as described by Danve et al., Vaccine (1993) 11(12):1214 against the meningococcal strain of origin of the polynucleotide or polypeptide used. In the field of meningococcal vaccines, the bactericidal activity assay is, in fact, recognized as being the reference assay based upon which it is possible to make a valid prediction of the vaccination value of a product. Briefly, a product according to the invention is administered to animals such as rabbits in order to produce an antiserum against this product. Then, this antiserum is assayed for its lysis capacity. The bactericidal titre of an antiserum represents the inverse of the dilution of this antiserum for which 50% of the load of meningococci is lysed. The antiserum is considered to be bactericidal when the titre is higher than 4, with respect to the menigococcal strain of origin of the polynucleotide or polypeptide used. In that case, the product against which the antiserum was generated is demonstrated to be potentially advantageous from a pharmaceutical point of view.

The following examples illustrate the invention without limiting the scope thereof.

LEGEND OF THE FIGURE

The attached FIGURE represents the vector pCAMyc-His used as a cloning vector.

DETAILS OF THE STRATEGY FOR IDENTIFYING THE ORFS

In order to select the ORF sequences specific for the pathogenic strains of the

Neisseria

genus, a PCR amplification is carried out on the sequences of the 118 ORFs selected after analysis with the Gene Jockey®, Codon Use®, and homology search programs. Only the sequences for which the amplification in

N. lactamica

is negative (sequences named “lactamica

−

”) are selected. In order to verify that these negative results are not “false negatives”, the lactamica

−

sequences selected are subjected to a dot blot.

A—PCR Amplification

A.1. Extraction of Genomic DNAs

The genomic DNAs of all of the

Neisseria

strains used in this study were prepared according to an identical protocol. The

N. meningitidis, N. lactamica, N. flava, N. subflava

and

N. mucosa

strains were cultured on tissues of MHA (Muller Hinton Agar, Difco) medium, and the

N. gonorrhoeae

were cultured on tissues of MHA medium supplemented with 10% of heat-treated horse blood (Biomérieux) and 1% of Isovitalex (Biomérieux). The culturing is carried out under an atmosphere containing 10% CO

2

, overnight at 37° C. Then, the cells are harvested, and washed in PBS phosphate buffer (pH 7.2), and the DNA is extracted according to protocol D of the “Rapid Prep genomic DNA isolation kit for cells and tissue” (Pharmacia Biotech).

The genomic DNAs were then controlled on agarose gel for their completeness and by PCR reaction for their purity.

A.2. PCR Reaction for Screening the ORFs Absent in

N. lactamica

2314

A PCR amplification was carried out on the genomic DNAs of the

N. meningitidis

strain ATCC 13090 and

N. lactamica

strain 2314 (ATCC 23970), according to the following protocol:

The PCR reaction was carried out on a 50 μl volume with 10 ng of genomic DNA, 250 μM of each of the dNTPs, 300 nM of each of the primers, 1×Taq DNA polymerase buffer and 2 u of Taq DNA polymerase (Appligène).

The amplification cycles are:

97° C.

45

seconds

25 cycles

56° C.

1

minute

25 cycles

72° C.

2.30

minutes

25 cycles

For each of the ORFs analysed, positive and negative controls for the PCR reaction were carried out. At this stage, only the

N. meningitidis+

and

N. lactamica−

ORFs are selected.

B—Selection of the

N. meningitidis

+

N. lactamica

−

ORFs by Dot Blot on Genomic DNA

The absence of a product of PCR amplification of an ORF with genomic DNA of

N. lactamica

2314 as the matrix does not guarantee the absence of this ORF in the

N. lactamica

2314 genome. Specifically, a certain variability in the region to which the oligonucleotides should hybridize may be responsible for the absence of amplified product for a given ORF.

In this context, further verification is carried out by dot blot on genomic DNA, using, as probe, the products of genomic amplification on the

N. meningitidis

strain corresponding to each of the reading frames identified. The dot blot filters contain genomic DNA of the following strains: 2

N. lactamica

strains 8064 and 2314, one

N. flava

strain ATCC 30008, one

N. mucosa

strain ATCC 9297, 3

N. meningitidis

serogroup B strains ATCC13090, M982 and B16B6, one

N. meningitidis

serogroup A strain Z2491, one

N. meningitidis

serogroup C strain (strain Z4182) and 2

N. gonorrhoeae

strains MS11 and FA1090. This dot blot analysis makes it possible to validate the absence of the ORF in

N. lactamica

2314 and 8064, and it is also an indication of the degree of variability of an ORF within the

Neisseria

strains.

The dot blot technique used is as follows. Approximately 50 ng of genomic DNA, denatured for 5 min at 100° C., of the various

Neisseria

strains are loaded, with suction, onto a Hybond N+nitrocellulose membrane (Amersham) placed between the jaws of a dot blot apparatus (BioRad). Then, the DNA is fixed on the membranes for 5 min with UV radiation at 315 nm.

The membranes are incubated in a prehybridization buffer (containing denatured salmon sperm DNA). They are then hybridized with a probe corresponding to the product of amplification of the ORF of interest, labelled according to a cold labelling protocol, such as the “DIG DNA labelling and detection kit” system (Boehringer Mannheim).

The ORF which does not hybridize to the genomic DNA of

N. lactamica

2314 and 8064 is definitively selected as a potential vaccination candidate.

EXAMPLE I

Cloning

1. PCR Amplification

Each of the ORFs was amplified by PCR using the genomic DNA of

N. meningitidis

serogroup B (strain ATCC 13090), according to standard protocol.

Two oligonucleotides, primers on the N-terminal side and on the C-terminal side were defined for each of the ORF sequences of the invention.

The primer on the N-terminal side comprises an enzyme restriction site for cloning, a CCACC Kozak sequence for translation initiation (M. Kozak, J. Mol. Biol. 196: 947-950), the ATG of the potential ORF and approximately 17 bases specific for the 5′ portion of the ORF.

The primer on the C-terminal side was defined such that the ORF cloned is in fusion, in its 3′ portion, with a repeat of 8 histidines and a stop codon which are present in the vector behind the multiple cloning site, hence the insertion of an “A” base in order to keep the correct reading frame after cloning and the disappearance of the stop codon of the ORF. The primer on the C-terminal side thus comprises an enzyme restriction site for cloning, an “A” base, and then approximately 20 bases specific for the 3′ portion of the gene starting from the codon preceding the stop codon.

After searching for restriction sites which are absent in each of the ORFs, with the aid of the DNASTAR MapDraw subprogram (Lasergene Software), the XbaI restriction site in 5′ and BglII restriction site in 3′ are used for the ORF SEQ ID No. 19. For the ORF SEQ ID No. 41, the SpeI site in 5′ and the BglII site in 3′ are used. The XbaI restriction site in 5′ and BamHI restriction site in 3′ are used to clone the remaining ORFs.

The PCR mixture comprises, for a final volume of 100 μl, 10-50 ng of genomic DNA, the N-terminal and C-terminal primers each at 200 nM, the dNTPs each at 250 μM, the 1×PCR buffer (composition of the 10 ×PCR buffer: 200 mM Tris-HCl (pH 8.8), 20 mM MgSO

4

, 100 mM KCl, 100 mM (NH

4

)

2

SO

4

, 1% TritonX-100 and 1 mg/ml of nuclease-free bovine serum albumin) and 2.5 U of polymerase.

The amplification is carried out as follows:

Temperature

Time

Number of

Step

(° C.)

(min.)

cycles

Denaturation

97

0.45

25

Hybridization

cf. table

1

25

Elongation

72

1/kb DNA

25

The primers used and the PCR conditions given in the table below, in which “N. gallelic variant” means that an allelic variant is present in

Neisseria gonorrhoeae

and “N. m A allelic variant” means that an allelic variant is present in

Neisseria meningitidis

serogroup A.

ORF

Hybri-

No.

diza-

(internal

tion

ref.)

SEQ ID No.

5′ Primer

3′ Primer

Polymerase

T °

22

1-2

GCT CTA GAC CAC CAT GTC TGA AGA

CGG GAT CCA GAA ATG GCT GGA TTC

Tfu

56° C.

N.g allelic variant:

AAA ATT GAA AAT GAG (SEQ ID no 78)

GCT ATC AG (SEQ ID no 79)

(Appligene)

54, 55

41

3-4

GCT CTA GAC CAC CAT GAA ACA CTT

CGG GAT CCA ATA CGT AGG ACT TGG

Tfu

43° C.

ACT CAT CG (SEQ ID no 80)

GTC (SEQ ID no 81)

(Appligene)

42-43

5-6

GCT CTA GAC CAC CAT GAA AAA ATC

CGG GAT CCA TTG CGG ATA AAC ATA

Tfu

56° C.

N.g allelic variant:

CCT TTT CGT TC (SEQ ID no 82)

TTC CGC C (SEQ ID no 83)

(Appligene)

56, 57

47

7-8

GCT CTA GAC CAC CAT GCG AAC GAC

CGG GAT CCA GAA CCG GTA GCC TAC

Tfu

56° C.

N.g allelic variant:

CCC AAC CTT C (SEQ ID no 84)

GCC GAC (SEQ ID no 85)

(Appligene)

58, 59

55

9-10

GCT CTA GAC CAC CAT GAA CAC ACG

CGG GAT CCA GCA ACG GCC TGC CGC

Pfu Turbo

56° C.

N.g allelic variant:

CAT CAT CGT TTC (SEQ ID no 86)

TTT AAG (SEQ ID no 87)

(Stratagene)

60, 61

68

11-12

GCT CTA GAC CAC CAT GCT GAC GTT

CGG GAT CCA CGG CAG AGG CAC GAT

Tfu

56° C.

TAT CGG ACT G (SEQ ID no 88)

TCC (SEQ ID no 89)

(Appligene)

71

13-14

GCT CTA GAC CAC CAT GGG CAT CCA

CGG GAT CCA CAA AAG TTC CAG AAA

Tfu

56° C.

TCT CGA CTT C (SEQ ID no 90)

ATC TAA CTC (SEQ ID no 91)

(Appligene)

72

15-16

GCT CTA GAC CAC CAT GAA TAG ACC

CGG GAT CCA TGC CGC TTG GGG GAG

Pfu Turbo

56° C.

N.mA. allelic

CAA GCA ACC (SEQ ID no 92)

GC (SEQ ID no 93)

(Stratagene)

variant: 62, 63

73

17-18

GCT CTA GAC CAC CAT GAT GAA TGT

CGG GAT CCA CAG TTT GCC CGA CAT

Pfu Turbo

56° C.

N.g allelic

CGA GGC AGA G (SEQ ID no 94)

AC (SEQ ID no 95)

(Stratagene)

variant:

64, 65

74

19-20

GCT CTA GAC CAC CAT GAA ATT TTT

GAA GAT CTA GAA ACT GTA ATT CAA

Pfu Turbo

56° C.

TCC TGC TCC (SEQ ID no 96)

GTT GAA G (SEQ ID no 97)

(Stratagene)

98

21-22

GCT CTA GAC CAC CAT GAT TGA ATT

CGG GAT CCA ACC CTG CGA CGA GTT

Pfu Turbo

56° C.

TGT CCG AGC (SEQ ID no 98)

GCG (SEQ ID no 99)

(Stratagene)

116

23-24

GCT CTA GAC CAC CAT GCA ATA CAG

CGG GAT CCA GTC CTT TTT CGC ACC

Pfu Turbo

56° C.

N.g. allelic

CAC ACT GGC (SEQ ID no 100)

TTG AAG (SEQ ID no 101)

(Stratagene)

variant:

66, 67

122

25-26

GCT CTA GAC CAC CAT GGA GCA GTC

CGG GAT CCA AGC TGT TTG GCG ATT

Pfu Turbo

56° C.

GGG CAA ATT C (SEQ ID no 102)

TCG GTG (SEQ ID no 103)

(Stratagene)

125

27-28

GCT CTA GAC CAC CAT GCA AAA CGG

CGG GAT CCA GTG CCT GCG CAG CTT

Pfu Turbo

56° C.

CGG GGG AAA G C (SEQ ID no 104)

GGA ATC (SEQ ID no 105)

(Stratagene)

128

29-30

GCT CTA GAC CAC CAT GAC ATT GCT

CGG GAT CCA TTC CGC AAA TAC CTG

Tfu

56° C.

N. mA. allelic

CAA TCT AAT GAT AAT G

TTT CCA ACC (SEQ ID no 107)

(Appligene)

variant: 68, 69

(SEQ ID no 106)

152

31-32

GCT CTA GAC CAC CAT GAA ACA ATC

CGG GAT CCA TAC TTG GGC GCA ACA

Pfu Turbo

56° C.

N.g allelic variant:

CGC CCG (SEQ ID no 108)

TGA C (SEQ ID no 109)

(Stratagene)

70, 71

153

33-34

GCT CTA GAC CAC CAT GAA TGT TTA

CGG GAT CCA TTT TTT AGA CGT ATT

Tfu

56° C.

CGG TTT CCC (SEQ ID no 110)

TTT AGT CG (SEQ ID no 111)

(Appligene)

155

35-36

GCT CTA GAC CAC CAT GAT GAG TCA

CGG GAT CCA TCC AGT TTT TGC TCG

Tfu

56° C.

ACA CTC TGC C (SEQ ID no 112)

AAG GC (SEQ ID no 113)

(Appligene)

156

37-38

GCT CTA GAC CAC CAT GCC TTC GAG

CGG GAT CCA TCG TTC TTC AAT CTC

Tfu

56° C.

CAA AAA CTG G (SEQ ID no 114)

CAC AAA CG (SEQ ID no 115)

(Appligene)

157

39-40

GCT CTA GAC CAC CAT GCA CC

CGG GAT CCA TTC AAT TCG CTT CAA

Tfu

56° C.

TGG AAA G (SEQ ID no 116)

CAA TG (SEQ ID no 117)

(Appligene)

158

41-42

GGA CTA GTC CAC CAT GGC TGC CAA

GAA GAT CTA AGC CGC GTT CCC TTC

Tfu

56° C.

N. mA. allelic

CCA ACG TTA CCG (SEQ ID no 118)

CAA AAA ATC (SEQ ID no 119)

(Appligene)

variant: 72, 73

159

43-44

GCT CTA GAC CAC CAT GCC GCA AAT

CGG GAT CCA AAA ACA ATC TTC CGG

Tfu

56° C.

N.mA. allelic

TAA AAT TCC C (SEQ ID no 120)

CAC CC (SEQ ID no 121)

(Appligene)

variant: 74, 75

161

45-46

GCT CTA GAC CAG CAT GCG CAC GCC

CGG GAT CCA TTG GGC AAC GAC GAA

Tfu

56° C.

GTT TTG TTG (SEQ ID no 122)

GGC AC (SEQ ID no 123)

(Appligene)

163-1

47-48

GCT CTA GAC CAC CAT GAG AAT

CGG GAT CCA TGG CTC AAT CCT

Pfu Turbo

56° C.

AGA GAT CAC ACC (SEQ ID no 124)

TTC TGC (SEQ ID no 125)

(Stratagene)

163-2

49-50

GCT CTA GAC CAC CAT GAT TCA CGT

CGG GAT CCA ACC TGC TTC ATG GGT

Tfu

56° C.

TTC GGC AGT G (SEQ ID no 126)

GAT TC (SEQ ID no 127)

(Appligene)

167-

51-52

GCT CTA GAC CAC CAT GAA TTC GAC

CGG GAT CCA AAT CCC TCT GCC GTA

Tfu

56° C.

168

N. gallelic variant:

CGC AAG TAA AAC (SEQ ID no 128)

TTT G (SEQ ID no 129)

(Appligene)

76, 77

2—Cloning, Transformation and Selection of Recombinants

The cloning vector used is the 6.357 kb vector pCA/Myc-His or pM10710 (cf. figure), derived from the plasmid pCDNA 3.1 (Invitrogen). pCA/Myc-His comprises, in particular, the CMV ie1 promoter (bases 249-902), intron A of the CMV ie1 gene (Chapman et al., 1991 Nucleic Acids Research, 19, 3979-3986), a multiple cloning site (bases 1792-1852) with the PmlI, EcoRV, NotI, XbaI, BamHI, KpnI and HindIII sites, a sequence encoding a polyhistidine and a stop codon (bases 1908-1928), a bgh 3′ termination sequence (bases 1853-2197) and the ampicillin resistance gene for selecting the recombinant clones in

E. coli.

After purification (GeneClean Bio101 kit), the PCR amplification products are digested for 2 hours at 37° C. with the appropriate enzymes (XbaI-BamHI, XbaI-BglII or SpeI-BglII), in a final reaction volume of 20 μl. The digestion products are then ligated with the vector pCA/Myc-His, digested beforehand with XbaI and BamHI, according to the “Rapid DNA Ligation Kit” protocol (Boehringer Mannheim). 15 μl of the ligation is used to transform 100 μl of competent

E. coli

XLI-blue cells (Novagen). The cells are incubated for 30 minutes in ice, 30 seconds at 42° C. and 2 minutes in ice. Then, 500 μl of LB medium without antibiotics are added, and the mixture is incubated for 1 hour at 37° C. Next, 50 and 550 μl of the culture are plated out on plates containing LB medium plus ampicillin (50 μg/ml final concentration), and incubated overnight at 37° C.

The following day, 36 colonies are placed in culture in 2 ml of LB plus ampicillin (50 μg/ml) and incubated overnight at 37° C.

The following day, the plasmid DNA is extracted according to the Qiagen mini-prep protocol (Qiagen) and the recombinants are identified by enzymatic restriction followed by agarose gel electrophoresis. The cloning junctions are then verified by sequencing.

EXAMPLE II

Evaluation of the Protective Activity of the ORFs of the Invention

A. Preparation of the DNA Intended for the Immunization Experiments

An isolated colony of a recombinant clone is used to inoculate a preculture in LB medium+ampicillin, and 5 ml of this preculture represents the inoculum of a 2.5 liter culture in LB medium+ampicillin. The purification protocol for preparing the plasmid DNA is that described in the EndoFree Giga Kit (Qiagen). The purified DNA is eluted from the purification column with a 10 mM Tris-HCl, 1 mM EDTA buffer, pH 8, and stored at −20° C. Before injection, the purified recombinant plasmid is diluted to 100 μg/ml with water (of injectable preparation quality) and the NaCl concentration is brought to 150 mM.

B. Production of a Specific Polyclonal Serum

B.1. Hyperimmunization in an Animal Model

The animal model used is the mouse or the rabbit. The route of administration of the injected DNA is the intramuscular or intradermal route. The recombinant plasmids to be injected are optionally applied to beads if they are injected into animals using a gene gun apparatus (BioRad). The immunization protocol follows a scheme comprising two injections, 3 weeks apart.

B.2. Analysis of the Bactericidal Activity of the Antibodies Induced

Ten days after the final injection, the animals are bled and the sera are analysed using the bactericidal activity assay according to the protocol of Danve et al., Vaccine (1993) 11 (12) :1214. Briefly, the sera are incubated at various dilutions (2-fold) in the presence of rabbit complement and of meningococci cultured in the presence or absence of an iron-chelating agent. The bactericidal titre of a serum represents the inverse of the dilution of this antiserum for which 50% of the bacteria are lysed.

It is considered that the antiserum is not bactericidal when its titre is lower than 4 against the homologous strain.

When the bactericidal titre corresponds to a 4-fold seroconversion against the homologous strain, the bactericidal activity of the antiserum is analysed against other

Neisseria

strains in order to measure the extent of the cross-reactivity of the antiserum of interest.

EXAMPLE III

Production of Purified Recombinant Proteins

1. Recombinant Production of Proteins

a. Preparation of Transformants

The PCR product obtained is then digested at 37° C. for two hours with restriction enzymes, in 20 μl of reaction volume. The digestion product is ligated into a plasmid pET28a (Novagen) which is cleaved in a similar way and which is dephosphorylated, before ligation, by treating with calf intestine alkaline phosphatase. The fusion gene constructed in this way allows the one-step affinity purification of the resulting fusion protein, due to the presence of histidine residues at the N-terminal end of the fusion protein, which are encoded by this vector.

The ligation reaction (20 μl) is carried out at 14° C. overnight, before transforming 100 μl of fresh competent

E. coli

XL1-blue cells (Novagen). The cells are incubated on ice for two hours, and then subjected to a heat shock at 42° C. for 30 seconds, before being returned to the ice for 90 seconds. The samples are then added to 1 ml of LB broth without selection, and cultured at 37° C. for two hours. The cells are then plated out on LB agar medium supplemented with kanamycin (50 μg/ml final concentration) at a 10×dilution, and are incubated overnight at 37° C. The following day, 50 colonies are subcultured on secondary plates and are incubated at 37° C. overnight.

b. Production of the Protein

The stored transformants (10 μl) are plated out onto selection plates and cultured overnight at 37° C. A few cells are harvested from the plate and used as an inoculum for an overnight starter culture (3 ml) at 37° C. The following day, a sample (time T=0) is taken and centrifuged at 14 000 rpm for 3 minutes. The starter culture is then used to inoculate an LB medium containing kanamycin (100 μg/ml) at a dilution of 1:50 (starting optical density OD

600

=0.05-0.1). The cells are cultured to an OD

600

of 1.0, a sample is taken for SDS-PAGE (pre-induction sample) and the remaining culture is induced with 1 mM of IPTG. The cultures are cultured for four hours and samples are taken every hour. The culture is centrifuged at 600 g for 20 minutes at 4° C. The supernatant is discarded and the pellets are resuspended in 50 mM of Tris-HCl (pH: 8.0), 2 mM EDTA, and recentrifuged. The supernatant is discarded and the cells are stored at −70° C.

2. Protein Purification

The pellets obtained from one liter of culture prepared according to Example I.4 above are dried and resuspended in 20 ml of 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 5 mM imidazole, cooled in ice. Lysozyme is added at a concentration of 0.1 mg/ml, and the suspension is homogenized using a high-speed homogenizer (Turrax), then treated with a sonicator (Sonifier 450, Branson). Benzonase (Merck) is used at a final concentration of 1 U/ml in order to eliminate the DNA. The suspension is centrifuged at 40 000 g for 20 minutes and the supernatant is filtered through a 0.45 μm membrane. The supernatant is loaded onto an IMAC column (12 ml of resin) which has been prepared by immobilizing Ni

++

cations according to the manufacturer's recommendations (Pharmacia). The column is washed with 10 column volumes of 20 mM Tris-HCl (pH 8.0), 0.5 M NaCl, 60 mM imidazole. The recombinant protein is eluted with six volumes of 20 mM Tris-HCl (pH: 7.9), 0.5 M NaCl, 500 mM imidazole, 0.1% Zwittergent 3-14.

The elution profile is controlled by measuring the absorbence of the fractions at an optical density of 280 nm. An aliquot fraction is analysed on an SDS-PAGE gel and stained with Coomassie blue (Phast System—Pharmacia), and the fractions corresponding to the protein peak are then pooled and concentrated. In order to eliminate the elution buffer, the fraction is passed over a G24 Sephadex column (Pharmacia) and equilibrated in PBS buffer (pH: 7.4). The protein solution is sterilized by filtration through a 0.45 μm membrane, and the protein concentration is determined using the BCA micromethod (Pierce). The protein solution is stored at −70° C.

EXAMPLE IV

Production of Monospecific Polyclonal Antibodies

1. Rabbit Hyperimmune Antiserum

100 μg (in total) of the polypeptide purified in Example III, in the presence of complete Freund's adjuvant in a total volume of approximately 2 ml, are injected into New Zealand rabbits, both subcutaneously and intravenously. 21 and 42 days after the initial injection, the booster doses, which are identical to the initial doses, are administered in the same way, with the exception that incomplete Freund's adjuvant is used. 15 days after the final injection, the animal's serum is recovered, decomplemented and filtered through a 0.45 μm membrane.

2. Mouse Hyperimmune Ascites Fluid

10-50 μg of the purified fusion polypeptide obtained in Example II, in the presence of complete Freund's adjuvant, in a volume of approximately 200 μl, are injected subcutaneously into 10 mice. 7 and 14 days after the initial injection, booster doses, which are identical to the initial doses, are administered in the same way, with the exception that incomplete Freund's adjuvant is used. 21 and 28 days after the initial injection, the mice receive 50 μg of the antigen alone, intraperitoneally. On the 21st day, the mice are also injected intraperitoneally with 180/TG CM26684 sarcoma cells (Lennette & Schmidt, Diagnostic procedures for viral, rickettsial, and chlamydial infections, (1979) 5th Ed. Washington D.C., American Public Health Association). The ascites fluids are harvested 10 to 13 days after the first injection.

EXAMPLE V

Purification of the Polypeptides of the Invention by Immunoaffinity

1. Purification of Specific IgG

An immune serum as prepared in Example IV is applied to a Fast Flow Sepharose 4 protein A column (Pharmacia) equilibrated with 100 mM Tris-HCl (pH: 8.0). The resin is washed by applying 10 column volumes of 100 mM Tris-HCl and 10 volumes of 10 mM Tris-HCl (pH: 8.0) to the column. The IgGs are eluted with a 0.1 M glycine buffer (pH: 3.0) and are collected by 5 ml fraction, to which 0.25 ml of 1 M Tris-HCl (pH: 8.0) are added. The optical density of the eluate is measured at 280 nm and the fractions containing the IgGs are pooled and, if necessary, stored at −70° C.

2. Column Preparation

A suitable amount of CNBr-activated Sepharose 4B gel (1 g of dried gel providing approximately 3.5 ml of hydrated gel, and the capacity of the gel ranging from 5 to 10 mg of coupled IgG per ml of gel) manufactured by Pharmacia (17-0430-01) is suspended in 1 mM HCl buffer and washed, using a Buchner funnel, by adding small amounts of 1 mM HCl buffer. The total volume of the buffer is 200 ml per gram of gel.

The purified IgGs are dialysed for four hours at 20±5° C. against 5 volumes of 500 mM PBS buffer (pH: 7.5). Then, they are diluted in 500 mM of PBS (pH: 7.5) for a final concentration of 3 mg/ml.

The IgGs are incubated with the gel overnight at 5±3° C., with stirring. The gel is packed into a chromatography column and washed with 2 column volumes of 500 mM phosphate buffer (pH: 7.5) and then one volume of 50 mM NaCl sodium buffer (pH: 7.5). The gel is then transferred to a tube, then incubated with 100 mM of ethanolamine (pH: 7.5) for 4 hours at room temperature with stirring, and then washed twice with two column volumes of PBS. The gel is then stored in PBS merthiolate at 1/10 000. The amount of IgG coupled to the gel is determined by measuring the optical density at 280 nm of the IgG solution and of the direct eluate.

3. Adsorption and Elution of the Antigen

A solution of antigen in 50 mM Tris-HCl (pH: 8.0), 2 mM EDTA, for example the supernatant obtained in Example III.2 after treatment with Benzonase, centrifugation and filtration through a 0.45 μm membrane, is applied to a column equilibrated with 50 mM Tris-HCl (pH: 8.0), 2 mM EDTA, at a flow rate of approximately 10 ml/hour. Then, the column is washed with 20 volumes of 50 mM Tris-HCl (pH: 8.0), 2 mM EDTA. Alternatively, batch adsorption can be carried out, in which the mixture is left overnight at 5±3° C., with stirring.

The gel is washed with 2 to 6 volumes of 10 mM PBS buffer (pH: 6.8). The antigen is eluted with a 100 mM glycine buffer (pH: 2.5). The eluate is collected in 3 ml fractions, to which 150 μl of 1 mM PBS buffer (pH: 8.0) are added. The optical density is measured at 280 nm for each fraction; those containing the antigen are recovered and stored at −20° C.

Fragments of the genome of

N. meningitidis

Z2491

described in patent application WO 98/02547

(2) INFORMATION FOR SEQ ID NO: 70A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 243 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 70A:

GATCAGACCC ATTTTCAGCG CACCGTAAGC GCGGATTTTC TCGAATTTTT CCAAAGCTGC

60

GGCATCGTTG TTGATGTCGT CTTGCAACTC TTTGCCCGTG TAGCCCAAGT CGGCGGCATT

120

CAGGAAAACG GTCGGAATGC CCGCGTTGAT GAGCGTGGCT TTCAAACGGC CTATATTCGG

180

CACATCAATT TCATCGACCA AATTGCCGGT TGGGAACATA CTGCCTTCGC CGTCGGCTGG

240

ATC

243

(2) INFORMATION FOR SEQ ID NO: 73A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 120 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 73A:

CGGTCAGAAA CAGGCAAGGT AATGAAAATG CCTGAGGCAC GGACTGTGCT GCGAACGAAA

60

ACTCCTTACC GAAGTCTTCT ATACCCAGGC TCAATAGCCG CTCAAGGAGA GAGCTATCAT

120

(2) INFORMATION FOR SEQ ID NO: 74A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 120 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 74A:

CGGTCAGAAA CAGGCAAGGT AATGAAAATG CCTGAGGCAC GGACTGTGCT GCGAACGAAA

60

ACTCCTTACC GAAGTCTTCT ATACCCAGGC TCAATAGCCG CTCAAGGAGA GAGCTATCAT

120

(2) INFORMATION FOR SEQ ID NO: 77A

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 269 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 77A:

CGGAGCATAA AATCGTTATT AAAGATAATG GTATAGGAAC GAGCTTCGAT GAAATCAATG

60

ATTTTTATTT GAGAATCGGT CGGAACAGAA GGGAAGAAAA ACAAGCCTCC CCGTGCGGAA

120

GAATTCCAAC GGGTAAAAAA GGCCTTGGTA AATTGGCATT ATTCGGGCTT GGCAACAAAA

180

TTGAAATTTC TACTATCCAG GGAAACGAAA GGGTTACTTT TACTTTGGAT TATGCAGAGA

240

TTCGAAGAAG CAAGGGTATT TATCAACCG

269

(2) INFORMATION FOR SEQ ID NO: 80A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 207 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 80A:

CGGGTCGCTT TATTTTGTGC AGGCATTATT TTTCATTTTT GGCTTGACAG TTTGGAAATA

60

TTGTGTATCG GGGGGGGGTA TTTGCTGACG TAAAAAACTA TAAACGCCGC GCAAAATATG

120

GCTGACTATA TTATTGACTT TGATTTTGTC CTGCGCGGTG ATGGATAAAA TCGCCAGCGA

180

TAAAGAATTT GCGAGAACCT GATGCCG

207

(2) INFORMATION FOR SEQ ID NO: 81A.:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 224 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: sing1e

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 81A:

CGGCAACGAT TTGAGCTATC GCCGTTACGA CATTCTGGAT TTGGCACAAA AATGCGAGTT

60

TGAAGAAGTC GCCCACCTGC TGATTCACGG CCATCTGCCC AACAAATTCG AGCTGGCCGC

120

TTATAAAACC AAGCTCAAAT CCATGCGCGG CCTGCCTATC CGTGTGATTA AAGTTTTGGA

180

AAGCCTGCCT GCACATACCC ATCCGATGGA CGTAATGCGT ACCG

224

(2) INFORMATION FOR SEQ ID NO: 87A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 273 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 87A:

AATTTCCACC TATGCCCTAC GCAGCGATTA TCCGTGGTTT ACCCAAAGGG TGATTATGGC

60

AAAAGCGCGG GGTTGAGCGA CCGCCTTTTG TTGCCGGCGT TCAAACGGGT TTTGATAGGA

120

AATGCAGGCA CGAAGCCTCG GCTGATTGTG ATGCACCTGA TGGGTTCGCA CAGTGATTTT

180

TGCACACGTT TGGATAAGGA TGCGCGGCGG TTTCAGTATC AAACTGAAAA AATATCCTGC

240

TATGTTTCCA TCAATCGCGC AAACCGATAA ATT

273

(2) INFORMATION FOR SEQ ID NO: 88A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 270 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 88A:

AATTCTTCCG CACGGGGAGG CTTGTTTTTC TTCCCTTCTG TTCCGACCGA TTCTCAAATA

60

AAAATCATTG ATTTCATCGA AGTTCATTCC TATACCATTA TCTTTAATAA CGATTTTATG

120

CTCCGGTTTA TCGAATAACC TAACTTCCAC TTCCGTAGCA CATGCATCGT AGGCATTCGC

180

TATCAACTCG CCAATCGCAG GAACAGTGTG CGAATACAAT CTTTACACCC AAATGTTCGA

240

TTACGGTTGG CTCGAAACTC AATTTCAATT

270

(2) INFORMATION FOR SEQ ID NO: 89A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 267 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 89A:

AATTATGAAC ACACGCATCA TCGTTTCGGC TGCGTTCGTT GCGTTGGCAT TAGCAGGTTG

60

CGGCTCAATC AATAATGTAA CCGTTTCCGA CCAGAAACTT CAGGAACGTG CCGCGTTTGC

120

CTTGGGCGTC ACCAATGCCG TAAAAATCAG CAACCGCAGC AATGAAGGCA TACGCATCAA

180

CTTTACCGCA ACTGTGGGTA AGCGCGTGAC CAATGCTATG TTACCAGTGT AATCAGCACA

240

ATCGGCGTTA CCACTTCCGA TGCAATT

267

(2) INFORMATION FOR SEQ ID NO: 94A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 308 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 94A:

AATTTGTTGG GCAGATGGCC GTGAATCAGC AGGTGGGCGA CTTCTTCAAA CTCGCATTTT

60

TCTGCCAAAT CCAGAATGTC GTAACCGCGA TACGTCAAAT CGTTGCCGGT ACGCAACGGT

120

ACACAAAGCG GTATTACCGG CCGCAACGCC AGAAAGCGCA ACGGATTTTT AGGTTTGAGG

180

GTCGGGGTTT GACTAGTTTC AGTCATGGTA TTTCTCCTTT GTGTTTTTAT GGGTTTCGGG

240

TTTTCAGACG ACCGATGCGG ATTTGTTGAA AGGCAGTCTG AAAGCGGTAA ATCATTTTTG

300

AAACAATT

308

(2) INFORMATION FOR SEQ ID NO: 95A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 286 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 95A:

AATTCGGAGG AGCAGTACCG CCAAGCGTTG CTCGCCTATT CCGGCGGTGA TAAAACAGAC

60

GAGGGTATCC GCCTGATGCA ACAGAGCGAT TACGGCAACT TGTCCTACCA CATCCGTAAT

120

AAAAACATGC TTTTCATTTT TTCGGCAAGC AATGACGCAC AAGCTCAGCC CAACACAACT

180

GACCCTATTG CCATTTTATG AAAAAGACGC TCAAAAAGGC ATTATCACAG TTGCAGGCGT

240

AGACCGCAGT GGAGAAAAGT TCAATGGCTC CAACCATTGC GGAATT

286

(2) INFORMATION FOR SEQ ID NO: 98A:

(i)

SEQUENCE CHARACTERISTICS:

(A) LENGTH: 316 base pairs

(B) TYPE: nucleotide

(C) STRANDEDNESS: single

(D) TOPOLOGY: linear

(ii)

MOLECULE TYPE: DNA (genomic)

(iii)

HYPOTHETICAL: NO

(iv)

ANTISENS: NO

(xi)

SEQUENCE DESCRIPTION: SEQ ID NO: 98A:

AATTTGTCGG CAATCTTCCC GGGTCGCTTT ATTTTGTGCA GGCATTATTT TTCATTTTTG

60

GCTTGACAGT TTGGAGATAT TGTGTATCGG GGGGGGGTAT TTGCTGACGT AAAAAACTAT

120

AAACGCCGCA GCAAAATATG GCTGACTATA TTATTGACTT TGATTTTGTC CTGCGCGGTG

180

ATGGATAAAA TCGCCAGCGA TAAAGATTTG CGAGAACCTG ATGCCGGCCT GTTGTTGAAT

240

ATTTTCGACC TGTAATTACG ATTTGGCTTC CGCGCCGGCA CAATATGCCG CCAAGCGGCG

300

CCCACATTTT GGAAGC

316

SEQUENCE LISTING

<160> NUMBER OF SEQ ID NOS: 129

<210> SEQ ID NO 1

<211> LENGTH: 858

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(855)

<400> SEQUENCE: 1

atg tct gaa gaa aaa ttg aaa atg agt ttc gag cca acc gta atc gaa 48

Met Ser Glu Glu Lys Leu Lys Met Ser Phe Glu Pro Thr Val Ile Glu

1 5 10 15

cat ttg ggt gta aag atg tat tcg cac act gtt cct gcg att gcc gag 96

His Leu Gly Val Lys Met Tyr Ser His Thr Val Pro Ala Ile Ala Glu

20 25 30

ttg ata gcg aat gcc tac gat gca tgt gct acg gaa gtg gaa gtt agg 144

Leu Ile Ala Asn Ala Tyr Asp Ala Cys Ala Thr Glu Val Glu Val Arg

35 40 45

tta ttc gat aaa ccg gag cat aaa atc gtt atc aaa gat aat ggt ata 192

Leu Phe Asp Lys Pro Glu His Lys Ile Val Ile Lys Asp Asn Gly Ile

50 55 60

gga atg agc ttc gat gaa atc aat gat ttt tat ttg aga atc ggt cgg 240

Gly Met Ser Phe Asp Glu Ile Asn Asp Phe Tyr Leu Arg Ile Gly Arg

65 70 75 80

aac aga agg gaa gaa aaa caa gct tcc ccg tgc gga aga att cca acg 288

Asn Arg Arg Glu Glu Lys Gln Ala Ser Pro Cys Gly Arg Ile Pro Thr

85 90 95

ggt aaa aaa ggc ctt ggt aaa ttg gca tta ttc ggg ctt ggc aac aaa 336

Gly Lys Lys Gly Leu Gly Lys Leu Ala Leu Phe Gly Leu Gly Asn Lys

100 105 110

att gaa att tct act atc cag gga aac gaa agg gtt act ttt act ttg 384

Ile Glu Ile Ser Thr Ile Gln Gly Asn Glu Arg Val Thr Phe Thr Leu

115 120 125

gat tat gca gag att cga aga agc aag ggt att tat caa ccg gag ttt 432

Asp Tyr Ala Glu Ile Arg Arg Ser Lys Gly Ile Tyr Gln Pro Glu Phe

130 135 140

cga aaa gaa tct gtt gaa tcc aat atc gaa agc ggg aca acc ata act 480

Arg Lys Glu Ser Val Glu Ser Asn Ile Glu Ser Gly Thr Thr Ile Thr

145 150 155 160

tta acc gaa ctg acg aaa aag caa gga tat ccg tta gat aat tat gta 528

Leu Thr Glu Leu Thr Lys Lys Gln Gly Tyr Pro Leu Asp Asn Tyr Val

165 170 175

gag cat ctt tcc cgc ttg ttt gat ttt ccg gct cag gat ttt aaa atc 576

Glu His Leu Ser Arg Leu Phe Asp Phe Pro Ala Gln Asp Phe Lys Ile

180 185 190

aaa gta agc ttg aac ggc tct gaa cct aaa atc att gat gga aat cta 624

Lys Val Ser Leu Asn Gly Ser Glu Pro Lys Ile Ile Asp Gly Asn Leu

195 200 205

aaa tat gat ctt gtt acc cca caa ttc gaa tgg gaa tac cag gat tta 672

Lys Tyr Asp Leu Val Thr Pro Gln Phe Glu Trp Glu Tyr Gln Asp Leu

210 215 220

gca acc aat att tca tcg tta tct tca aaa ttc gaa cag tat gaa tac 720

Ala Thr Asn Ile Ser Ser Leu Ser Ser Lys Phe Glu Gln Tyr Glu Tyr

225 230 235 240

agc gga tta ata caa ggt aag ttc att aca acg gaa aaa cct tta aag 768

Ser Gly Leu Ile Gln Gly Lys Phe Ile Thr Thr Glu Lys Pro Leu Lys

245 250 255

aat aat atg aaa ggt att acc ttg ttt gcc aac ggc aga atg gta aat 816

Asn Asn Met Lys Gly Ile Thr Leu Phe Ala Asn Gly Arg Met Val Asn

260 265 270

atg ccc gag ttt ttc act gat agc gaa tcc agc cat ttc taa 858

Met Pro Glu Phe Phe Thr Asp Ser Glu Ser Ser His Phe

275 280 285

<210> SEQ ID NO 2

<211> LENGTH: 285

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 2

Met Ser Glu Glu Lys Leu Lys Met Ser Phe Glu Pro Thr Val Ile Glu

1 5 10 15

His Leu Gly Val Lys Met Tyr Ser His Thr Val Pro Ala Ile Ala Glu

20 25 30

Leu Ile Ala Asn Ala Tyr Asp Ala Cys Ala Thr Glu Val Glu Val Arg

35 40 45

Leu Phe Asp Lys Pro Glu His Lys Ile Val Ile Lys Asp Asn Gly Ile

50 55 60

Gly Met Ser Phe Asp Glu Ile Asn Asp Phe Tyr Leu Arg Ile Gly Arg

65 70 75 80

Asn Arg Arg Glu Glu Lys Gln Ala Ser Pro Cys Gly Arg Ile Pro Thr

85 90 95

Gly Lys Lys Gly Leu Gly Lys Leu Ala Leu Phe Gly Leu Gly Asn Lys

100 105 110

Ile Glu Ile Ser Thr Ile Gln Gly Asn Glu Arg Val Thr Phe Thr Leu

115 120 125

Asp Tyr Ala Glu Ile Arg Arg Ser Lys Gly Ile Tyr Gln Pro Glu Phe

130 135 140

Arg Lys Glu Ser Val Glu Ser Asn Ile Glu Ser Gly Thr Thr Ile Thr

145 150 155 160

Leu Thr Glu Leu Thr Lys Lys Gln Gly Tyr Pro Leu Asp Asn Tyr Val

165 170 175

Glu His Leu Ser Arg Leu Phe Asp Phe Pro Ala Gln Asp Phe Lys Ile

180 185 190

Lys Val Ser Leu Asn Gly Ser Glu Pro Lys Ile Ile Asp Gly Asn Leu

195 200 205

Lys Tyr Asp Leu Val Thr Pro Gln Phe Glu Trp Glu Tyr Gln Asp Leu

210 215 220

Ala Thr Asn Ile Ser Ser Leu Ser Ser Lys Phe Glu Gln Tyr Glu Tyr

225 230 235 240

Ser Gly Leu Ile Gln Gly Lys Phe Ile Thr Thr Glu Lys Pro Leu Lys

245 250 255

Asn Asn Met Lys Gly Ile Thr Leu Phe Ala Asn Gly Arg Met Val Asn

260 265 270

Met Pro Glu Phe Phe Thr Asp Ser Glu Ser Ser His Phe

275 280 285

<210> SEQ ID NO 3

<211> LENGTH: 1101

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1098)

<400> SEQUENCE: 3

atg aaa cac tta ctc atc gat ttt gaa aac gtc cag ccg caa aac tta 48

Met Lys His Leu Leu Ile Asp Phe Glu Asn Val Gln Pro Gln Asn Leu

1 5 10 15

gac aaa tta cta acc gaa aat acc cat att tgg cta ttt ata ggt gta 96

Asp Lys Leu Leu Thr Glu Asn Thr His Ile Trp Leu Phe Ile Gly Val

20 25 30

tta cac aaa atg tta cct att agt ctg gtg caa tcc cta cta cgt ttc 144

Leu His Lys Met Leu Pro Ile Ser Leu Val Gln Ser Leu Leu Arg Phe

35 40 45

ggc gaa cgt gtc cat ctt gtc cag tta caa aaa acg ggg aaa aac gca 192

Gly Glu Arg Val His Leu Val Gln Leu Gln Lys Thr Gly Lys Asn Ala

50 55 60

ttg gat ttt tac ctg tcc tat tac ctc gga caa att acc gcc aca gac 240

Leu Asp Phe Tyr Leu Ser Tyr Tyr Leu Gly Gln Ile Thr Ala Thr Asp

65 70 75 80

ccc aat gcc caa atc ggc ata ctc tcg cgt gat gga gga tac gat gtt 288

Pro Asn Ala Gln Ile Gly Ile Leu Ser Arg Asp Gly Gly Tyr Asp Val

85 90 95

ctg gtc gaa cat att ttg aaa aac cac cag gcg aag ggt atc gtg cgc 336

Leu Val Glu His Ile Leu Lys Asn His Gln Ala Lys Gly Ile Val Arg

100 105 110

cta gcc aat ata gat gaa gta caa cat cag aaa att gct acc gaa ccg 384

Leu Ala Asn Ile Asp Glu Val Gln His Gln Lys Ile Ala Thr Glu Pro

115 120 125

ccg tca gca ttg ctg gaa aac act cct cag cct gaa acc acc ctc aaa 432

Pro Ser Ala Leu Leu Glu Asn Thr Pro Gln Pro Glu Thr Thr Leu Lys

130 135 140

cca cag caa cca tta act tcc tat ttc caa gca gcc cta act gca ctg 480

Pro Gln Gln Pro Leu Thr Ser Tyr Phe Gln Ala Ala Leu Thr Ala Leu

145 150 155 160

cgc cgc ccc gac gct ttc cgc ccc tgc cgc ctg cat aac ctg cga caa 528

Arg Arg Pro Asp Ala Phe Arg Pro Cys Arg Leu His Asn Leu Arg Gln

165 170 175

aat ctg cgt aag cat att ttg agt gat ttg ttt aaa gaa aaa acc gat 576

Asn Leu Arg Lys His Ile Leu Ser Asp Leu Phe Lys Glu Lys Thr Asp

180 185 190

gaa gaa tgc gaa ata acc act gct aac gtt atc aat aaa ctc aaa gca 624

Glu Glu Cys Glu Ile Thr Thr Ala Asn Val Ile Asn Lys Leu Lys Ala

195 200 205

caa aac ttc atc agc att gat gaa cag gaa acc gtt tcc tac cat ctc 672

Gln Asn Phe Ile Ser Ile Asp Glu Gln Glu Thr Val Ser Tyr His Leu

210 215 220

agt gat aat gat ttg tta caa aga atc caa cgc cat att tta agc caa 720

Ser Asp Asn Asp Leu Leu Gln Arg Ile Gln Arg His Ile Leu Ser Gln

225 230 235 240

cgt ccc aaa acc tac gct gat ttt caa gcc gtc gtg caa aac cga gca 768

Arg Pro Lys Thr Tyr Ala Asp Phe Gln Ala Val Val Gln Asn Arg Ala

245 250 255

gat gca ctt cac tta aca gtc ggt acc aac gac att caa tcc ttt gcg 816

Asp Ala Leu His Leu Thr Val Gly Thr Asn Asp Ile Gln Ser Phe Ala

260 265 270

cga cat ttg cgc gac caa aac ctg atc cgc caa aac aat ggg aaa att 864

Arg His Leu Arg Asp Gln Asn Leu Ile Arg Gln Asn Asn Gly Lys Ile

275 280 285

gaa tat gca ccg ttt act gaa cct aaa cca cag cca acg ccc aag cag 912

Glu Tyr Ala Pro Phe Thr Glu Pro Lys Pro Gln Pro Thr Pro Lys Gln

290 295 300

cct aaa aaa acc gca tgg gaa cct gat gaa att att tgg aaa aaa gtg 960

Pro Lys Lys Thr Ala Trp Glu Pro Asp Glu Ile Ile Trp Lys Lys Val

305 310 315 320

att gcc gcg tta tcg tta aag aac cgt cct aat aaa acc aaa act tta 1008

Ile Ala Ala Leu Ser Leu Lys Asn Arg Pro Asn Lys Thr Lys Thr Leu

325 330 335

cgc aat aca atc cag gca ctc aca aaa tcc aat gca caa gaa act gac 1056

Arg Asn Thr Ile Gln Ala Leu Thr Lys Ser Asn Ala Gln Glu Thr Asp

340 345 350

aaa ctg cta caa cat tta caa gat gac cca agt cct acg tat tga 1101

Lys Leu Leu Gln His Leu Gln Asp Asp Pro Ser Pro Thr Tyr

355 360 365

<210> SEQ ID NO 4

<211> LENGTH: 366

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 4

Met Lys His Leu Leu Ile Asp Phe Glu Asn Val Gln Pro Gln Asn Leu

1 5 10 15

Asp Lys Leu Leu Thr Glu Asn Thr His Ile Trp Leu Phe Ile Gly Val

20 25 30

Leu His Lys Met Leu Pro Ile Ser Leu Val Gln Ser Leu Leu Arg Phe

35 40 45

Gly Glu Arg Val His Leu Val Gln Leu Gln Lys Thr Gly Lys Asn Ala

50 55 60

Leu Asp Phe Tyr Leu Ser Tyr Tyr Leu Gly Gln Ile Thr Ala Thr Asp

65 70 75 80

Pro Asn Ala Gln Ile Gly Ile Leu Ser Arg Asp Gly Gly Tyr Asp Val

85 90 95

Leu Val Glu His Ile Leu Lys Asn His Gln Ala Lys Gly Ile Val Arg

100 105 110

Leu Ala Asn Ile Asp Glu Val Gln His Gln Lys Ile Ala Thr Glu Pro

115 120 125

Pro Ser Ala Leu Leu Glu Asn Thr Pro Gln Pro Glu Thr Thr Leu Lys

130 135 140

Pro Gln Gln Pro Leu Thr Ser Tyr Phe Gln Ala Ala Leu Thr Ala Leu

145 150 155 160

Arg Arg Pro Asp Ala Phe Arg Pro Cys Arg Leu His Asn Leu Arg Gln

165 170 175

Asn Leu Arg Lys His Ile Leu Ser Asp Leu Phe Lys Glu Lys Thr Asp

180 185 190

Glu Glu Cys Glu Ile Thr Thr Ala Asn Val Ile Asn Lys Leu Lys Ala

195 200 205

Gln Asn Phe Ile Ser Ile Asp Glu Gln Glu Thr Val Ser Tyr His Leu

210 215 220

Ser Asp Asn Asp Leu Leu Gln Arg Ile Gln Arg His Ile Leu Ser Gln

225 230 235 240

Arg Pro Lys Thr Tyr Ala Asp Phe Gln Ala Val Val Gln Asn Arg Ala

245 250 255

Asp Ala Leu His Leu Thr Val Gly Thr Asn Asp Ile Gln Ser Phe Ala

260 265 270

Arg His Leu Arg Asp Gln Asn Leu Ile Arg Gln Asn Asn Gly Lys Ile

275 280 285

Glu Tyr Ala Pro Phe Thr Glu Pro Lys Pro Gln Pro Thr Pro Lys Gln

290 295 300

Pro Lys Lys Thr Ala Trp Glu Pro Asp Glu Ile Ile Trp Lys Lys Val

305 310 315 320

Ile Ala Ala Leu Ser Leu Lys Asn Arg Pro Asn Lys Thr Lys Thr Leu

325 330 335

Arg Asn Thr Ile Gln Ala Leu Thr Lys Ser Asn Ala Gln Glu Thr Asp

340 345 350

Lys Leu Leu Gln His Leu Gln Asp Asp Pro Ser Pro Thr Tyr

355 360 365

<210> SEQ ID NO 5

<211> LENGTH: 1572

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1569)

<400> SEQUENCE: 5

atg aaa aaa tcc ctt ttc gtt ctc ttt ctg tat tca tcc cta ctt acc 48

Met Lys Lys Ser Leu Phe Val Leu Phe Leu Tyr Ser Ser Leu Leu Thr

1 5 10 15

gcc agc gaa atc gcc tat cgc ttt gta ttc gga att gaa acc tta ccg 96

Ala Ser Glu Ile Ala Tyr Arg Phe Val Phe Gly Ile Glu Thr Leu Pro

20 25 30

gct gca aaa atg gca gaa acg ttt gcg ctg aca ttt atg att gct gcg 144

Ala Ala Lys Met Ala Glu Thr Phe Ala Leu Thr Phe Met Ile Ala Ala

35 40 45

ctg tat ctg ttt gcg cgt tat aag gct tcg cgg ctg ctg att gcg gtg 192

Leu Tyr Leu Phe Ala Arg Tyr Lys Ala Ser Arg Leu Leu Ile Ala Val

50 55 60

ttt ttc gcg ttc agc att att gcc aac aat gta cat tat gcg gtt tat 240

Phe Phe Ala Phe Ser Ile Ile Ala Asn Asn Val His Tyr Ala Val Tyr

65 70 75 80

caa agt tgg atg acg ggc atc aat tat tgg ctg atg ctg aaa gag att 288

Gln Ser Trp Met Thr Gly Ile Asn Tyr Trp Leu Met Leu Lys Glu Ile

85 90 95

acc gaa gtc ggc agt gcg ggc gcg tcg atg ttg gat aag ttg tgg ctg 336

Thr Glu Val Gly Ser Ala Gly Ala Ser Met Leu Asp Lys Leu Trp Leu

100 105 110

cct gcg ttg tgg ggc gtg ttg gaa gtc atg ttg ttt tgc agc ctt gcc 384

Pro Ala Leu Trp Gly Val Leu Glu Val Met Leu Phe Cys Ser Leu Ala

115 120 125

aag ttc cac cgt aag acg cat ttt tct gcc gat ata ctg ttt gcc ttc 432

Lys Phe His Arg Lys Thr His Phe Ser Ala Asp Ile Leu Phe Ala Phe

130 135 140

cta atg ctg atg att ttc gtg cgt tcg ttc gac acg aaa caa gag cac 480

Leu Met Leu Met Ile Phe Val Arg Ser Phe Asp Thr Lys Gln Glu His

145 150 155 160

ggt att tcg ccc aaa ccg aca tac agc cgc atc aaa gcc aat tat ttc 528

Gly Ile Ser Pro Lys Pro Thr Tyr Ser Arg Ile Lys Ala Asn Tyr Phe

165 170 175

agc ttc ggt tat ttt gtc gga cgc gtg ttg ccg tat cag ttg ttt gat 576

Ser Phe Gly Tyr Phe Val Gly Arg Val Leu Pro Tyr Gln Leu Phe Asp

180 185 190

tta agc agg att ccc gcc ttt aag cag cct gct cca agc aaa atc ggg 624

Leu Ser Arg Ile Pro Ala Phe Lys Gln Pro Ala Pro Ser Lys Ile Gly

195 200 205

cag ggc agt gtt caa aat atc gtc ctg att atg ggc gaa agc gaa agc 672

Gln Gly Ser Val Gln Asn Ile Val Leu Ile Met Gly Glu Ser Glu Ser

210 215 220

gcg gcg cat ttg aag ctg ttt ggc tac gga cgc gaa act tcg ccg ttt 720

Ala Ala His Leu Lys Leu Phe Gly Tyr Gly Arg Glu Thr Ser Pro Phe

225 230 235 240

tta acc cgg ctg tcg caa gcc gat ttt aag ccg att gtg aaa caa agt 768

Leu Thr Arg Leu Ser Gln Ala Asp Phe Lys Pro Ile Val Lys Gln Ser

245 250 255

tat tcc gca ggc ttt atg act gca gtg tcc ctg ccc agt ttt ttc aat 816

Tyr Ser Ala Gly Phe Met Thr Ala Val Ser Leu Pro Ser Phe Phe Asn

260 265 270

gcg ata ccg cac gcc aac ggc ttg gaa caa atc agc ggc ggc gat act 864

Ala Ile Pro His Ala Asn Gly Leu Glu Gln Ile Ser Gly Gly Asp Thr

275 280 285

aat atg ttc cgc ctc gcc aaa gag cag ggc tat gaa acg tat ttt tac 912

Asn Met Phe Arg Leu Ala Lys Glu Gln Gly Tyr Glu Thr Tyr Phe Tyr

290 295 300

agc gca cag gcg gaa aac gag atg gcg att ttg aac tta atc ggt aag 960

Ser Ala Gln Ala Glu Asn Glu Met Ala Ile Leu Asn Leu Ile Gly Lys

305 310 315 320

aaa tgg ata gac cat ctg att cag ccg acg cag ctt ggc tac ggc aac 1008

Lys Trp Ile Asp His Leu Ile Gln Pro Thr Gln Leu Gly Tyr Gly Asn

325 330 335

ggc gac aat atg ccc gat gag aag ctg ctg ccg ctg ttc gac aaa atc 1056

Gly Asp Asn Met Pro Asp Glu Lys Leu Leu Pro Leu Phe Asp Lys Ile

340 345 350

aat ttg cag cag ggc agg cat ttt atc gtg ttg cac caa cgt ggt tcg 1104

Asn Leu Gln Gln Gly Arg His Phe Ile Val Leu His Gln Arg Gly Ser

355 360 365

cac gcc cca tac agc gca ttg ttg cag cct caa gat aaa gta ttc ggc 1152

His Ala Pro Tyr Ser Ala Leu Leu Gln Pro Gln Asp Lys Val Phe Gly

370 375 380

gaa ctt att gtg gat aag tac gac aac acc atc cac aaa acc gac caa 1200

Glu Leu Ile Val Asp Lys Tyr Asp Asn Thr Ile His Lys Thr Asp Gln

385 390 395 400

atg att caa acc gta ttc gag cag ctg caa aag cag cct gac ggc aac 1248

Met Ile Gln Thr Val Phe Glu Gln Leu Gln Lys Gln Pro Asp Gly Asn

405 410 415

tgg ctg ttt gcc tat acc tcc gat cat ggc cag tat gtt cgc caa gat 1296

Trp Leu Phe Ala Tyr Thr Ser Asp His Gly Gln Tyr Val Arg Gln Asp

420 425 430

atc tac aat caa ggc acg gtg cag ccc gac agc tat ctc gtg ccg ctg 1344

Ile Tyr Asn Gln Gly Thr Val Gln Pro Asp Ser Tyr Leu Val Pro Leu

435 440 445

gtg ttg tac agc tcg aat aag gcc gtg caa cag gct gcc aac cag gct 1392

Val Leu Tyr Ser Ser Asn Lys Ala Val Gln Gln Ala Ala Asn Gln Ala

450 455 460

ttt gcg cct tgc gag att gcc ttc cat cag cag ctt tca acg ttc ctg 1440

Phe Ala Pro Cys Glu Ile Ala Phe His Gln Gln Leu Ser Thr Phe Leu

465 470 475 480

att cac acg ttg ggc tac gat atg ccg gtt tca ggt tgt cgc gaa ggc 1488

Ile His Thr Leu Gly Tyr Asp Met Pro Val Ser Gly Cys Arg Glu Gly

485 490 495

tcg gta acg ggc aac ctg att acg ggt gat gca ggc agc ttg aac att 1536

Ser Val Thr Gly Asn Leu Ile Thr Gly Asp Ala Gly Ser Leu Asn Ile

500 505 510

cgc gac ggc aag gcg gaa tat gtt tat ccg caa tga 1572

Arg Asp Gly Lys Ala Glu Tyr Val Tyr Pro Gln

515 520

<210> SEQ ID NO 6

<211> LENGTH: 523

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 6

Met Lys Lys Ser Leu Phe Val Leu Phe Leu Tyr Ser Ser Leu Leu Thr

1 5 10 15

Ala Ser Glu Ile Ala Tyr Arg Phe Val Phe Gly Ile Glu Thr Leu Pro

20 25 30

Ala Ala Lys Met Ala Glu Thr Phe Ala Leu Thr Phe Met Ile Ala Ala

35 40 45

Leu Tyr Leu Phe Ala Arg Tyr Lys Ala Ser Arg Leu Leu Ile Ala Val

50 55 60

Phe Phe Ala Phe Ser Ile Ile Ala Asn Asn Val His Tyr Ala Val Tyr

65 70 75 80

Gln Ser Trp Met Thr Gly Ile Asn Tyr Trp Leu Met Leu Lys Glu Ile

85 90 95

Thr Glu Val Gly Ser Ala Gly Ala Ser Met Leu Asp Lys Leu Trp Leu

100 105 110

Pro Ala Leu Trp Gly Val Leu Glu Val Met Leu Phe Cys Ser Leu Ala

115 120 125

Lys Phe His Arg Lys Thr His Phe Ser Ala Asp Ile Leu Phe Ala Phe

130 135 140

Leu Met Leu Met Ile Phe Val Arg Ser Phe Asp Thr Lys Gln Glu His

145 150 155 160

Gly Ile Ser Pro Lys Pro Thr Tyr Ser Arg Ile Lys Ala Asn Tyr Phe

165 170 175

Ser Phe Gly Tyr Phe Val Gly Arg Val Leu Pro Tyr Gln Leu Phe Asp

180 185 190

Leu Ser Arg Ile Pro Ala Phe Lys Gln Pro Ala Pro Ser Lys Ile Gly

195 200 205

Gln Gly Ser Val Gln Asn Ile Val Leu Ile Met Gly Glu Ser Glu Ser

210 215 220

Ala Ala His Leu Lys Leu Phe Gly Tyr Gly Arg Glu Thr Ser Pro Phe

225 230 235 240

Leu Thr Arg Leu Ser Gln Ala Asp Phe Lys Pro Ile Val Lys Gln Ser

245 250 255

Tyr Ser Ala Gly Phe Met Thr Ala Val Ser Leu Pro Ser Phe Phe Asn

260 265 270

Ala Ile Pro His Ala Asn Gly Leu Glu Gln Ile Ser Gly Gly Asp Thr

275 280 285

Asn Met Phe Arg Leu Ala Lys Glu Gln Gly Tyr Glu Thr Tyr Phe Tyr

290 295 300

Ser Ala Gln Ala Glu Asn Glu Met Ala Ile Leu Asn Leu Ile Gly Lys

305 310 315 320

Lys Trp Ile Asp His Leu Ile Gln Pro Thr Gln Leu Gly Tyr Gly Asn

325 330 335

Gly Asp Asn Met Pro Asp Glu Lys Leu Leu Pro Leu Phe Asp Lys Ile

340 345 350

Asn Leu Gln Gln Gly Arg His Phe Ile Val Leu His Gln Arg Gly Ser

355 360 365

His Ala Pro Tyr Ser Ala Leu Leu Gln Pro Gln Asp Lys Val Phe Gly

370 375 380

Glu Leu Ile Val Asp Lys Tyr Asp Asn Thr Ile His Lys Thr Asp Gln

385 390 395 400

Met Ile Gln Thr Val Phe Glu Gln Leu Gln Lys Gln Pro Asp Gly Asn

405 410 415

Trp Leu Phe Ala Tyr Thr Ser Asp His Gly Gln Tyr Val Arg Gln Asp

420 425 430

Ile Tyr Asn Gln Gly Thr Val Gln Pro Asp Ser Tyr Leu Val Pro Leu

435 440 445

Val Leu Tyr Ser Ser Asn Lys Ala Val Gln Gln Ala Ala Asn Gln Ala

450 455 460

Phe Ala Pro Cys Glu Ile Ala Phe His Gln Gln Leu Ser Thr Phe Leu

465 470 475 480

Ile His Thr Leu Gly Tyr Asp Met Pro Val Ser Gly Cys Arg Glu Gly

485 490 495

Ser Val Thr Gly Asn Leu Ile Thr Gly Asp Ala Gly Ser Leu Asn Ile

500 505 510

Arg Asp Gly Lys Ala Glu Tyr Val Tyr Pro Gln

515 520

<210> SEQ ID NO 7

<211> LENGTH: 3204

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(3201)

<400> SEQUENCE: 7

atg cga acg acc cca acc ttc cct aca aaa act ttc aaa ccg gct gcc 48

Met Arg Thr Thr Pro Thr Phe Pro Thr Lys Thr Phe Lys Pro Ala Ala

1 5 10 15

atg gcg tta gct gtt gca aca aca ctt tct gcc tgc tta ggc ggc ggc 96

Met Ala Leu Ala Val Ala Thr Thr Leu Ser Ala Cys Leu Gly Gly Gly

20 25 30

ggc ggc act tct gcg ccc gac ttc aat gca ggc ggc acc ggt atc ggc 144

Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly

35 40 45

agc aac agc aga gca aca aca gcg aaa tca gca gca gta tct tac gcc 192

Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala

50 55 60

ggt atc aag aac gaa atg tgc aaa gac aga agc atg ctc tgt gcc ggt 240

Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly

65 70 75 80

cgg gat gac gtt gcg gtt aca gac agg gat gcc aaa atc aat gcc ccc 288

Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro

85 90 95

ccc ccg aat ctg cat acc gga gac ttt aca aac cca aat gac gca tac 336

Pro Pro Asn Leu His Thr Gly Asp Phe Thr Asn Pro Asn Asp Ala Tyr

100 105 110

aag aat ttg atc aac ctc aaa cct gca att gaa gca ggc tat aca gga 384

Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly

115 120 125

cgc ggg gta gag gta ggt atc gtc gat aca ggc gaa tcc gtc ggc agc 432

Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser

130 135 140

ata tcc ttt ccc gaa ctg tat ggc aga aaa gaa cac ggc tat aac gaa 480

Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu

145 150 155 160

aat tac aaa aac tat acg gcg tat atg cgg aag gaa gcg cct gaa gac 528

Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp

165 170 175

gga ggc ggt aaa gac att aaa gct tct ttc gac gat gag gcc gtt ata 576

Gly Gly Gly Lys Asp Ile Lys Ala Ser Phe Asp Asp Glu Ala Val Ile

180 185 190

gag act gaa gca aag ccg acg gat atc cgc cac gta aaa gaa atc gga 624

Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly

195 200 205

cac atc gat gtg gtc tcc cat att att ggc ggg cgt tcc gtg gac ggc 672

His Ile Asp Val Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly

210 215 220

aga cct gca ggc ggt att gcg ccc gat gcg acg cta cac ata atg aat 720

Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn

225 230 235 240

acg cat gat gga acc aag aac gaa ata atg tct gca gcc atc cgc aat 768

Thr His Asp Gly Thr Lys Asn Glu Ile Met Ser Ala Ala Ile Arg Asn

245 250 255

gca tgg gtc aag ctg ggc gaa cgt ggc gtg cgc atc gtc aat aac agt 816

Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser

260 265 270

ttt gga aca aca tcg agg gca ggc act gcc gac cat ttc caa ata gcc 864

Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp His Phe Gln Ile Ala

275 280 285

aat tcg gag gag cag tac cgc caa gcg ttg ctc gcc tat tcc ggc ggt 912

Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Ala Tyr Ser Gly Gly

290 295 300

gat aaa aca gac gag ggt atc cgc ctg atg caa cag agc gat tac ggc 960

Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly

305 310 315 320

aac ttg tcc tac cac atc cgt aat aaa aac atg ctt ttc att ttt tcg 1008

Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser

325 330 335

gca agc aat gac gca caa gct cag ccc aac aca ctg acc cta ttg cca 1056

Ala Ser Asn Asp Ala Gln Ala Gln Pro Asn Thr Leu Thr Leu Leu Pro

340 345 350

ttt tat gaa aaa gat gct caa aaa ggc att atc aca gtc gca ggc gta 1104

Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val

355 360 365

gac cgc agt gga gaa aag ttc aat ggc tcc aac cat tgc gga att act 1152

Asp Arg Ser Gly Glu Lys Phe Asn Gly Ser Asn His Cys Gly Ile Thr

370 375 380

gcc atg tgg tgc cta tcg gca ccc tat gaa gca agc gtc cgt ttc acc 1200

Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr

385 390 395 400

cgt aca aac ccg att caa att gcc gga aca tcc ttt tcc gca ccc atc 1248

Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile

405 410 415

gta acc ggc acg gcg gct ctg ctg ctg cag aaa tac ccg tgg atg agc 1296

Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser

420 425 430

aac gac aac ctg cgt acc acg ctg ctg aca acg gct cag gac atc ggt 1344

Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly

435 440 445

gca gtc ggc gtg gac agc aag ttc ggc tgg gga ctg ctg gat gcg ggt 1392

Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly

450 455 460

aag gcc atg aac gga ccc gcg tcc ttt ccg ttc ggc gac ttt acc gcc 1440

Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala

465 470 475 480

gat acg aaa ggt aca tcc gat att gcc tac tcc ttc cgt aac gac att 1488

Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile

485 490 495

tca ggc acg ggc ggc ctg atc aaa aaa ggc ggc agc caa ctg caa ctg 1536

Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu

500 505 510

cac ggc aac aac acc tat acg ggc aaa acc att atc gaa ggc ggt tcg 1584

His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser

515 520 525

ctg gtg ttg tac ggc aac aac aaa tcg gat atg cgc gtc gaa acc aaa 1632

Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys

530 535 540

ggt gcg ctg att tat aac ggg gcg gca tcc ggc ggt agc ctg aac agc 1680

Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser

545 550 555 560

gac ggc att gtc tat ctg gca gat acc gac cga tcc ggc gca aac gaa 1728

Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Arg Ser Gly Ala Asn Glu

565 570 575

acc gtg cac atc aaa ggc gat ctg cag ctg ggc ggc gaa ggt acg ctg 1776

Thr Val His Ile Lys Gly Asp Leu Gln Leu Gly Gly Glu Gly Thr Leu

580 585 590

tac aca cgt ttg ggc aaa ctg ctg aaa gtg gac ggt acg gcg atg acc 1824

Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Met Thr

595 600 605

ggc ggc aag ctg tac atg tcg gca cgc ggc aaa ggg gca ggc tat ctc 1872

Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu

610 615 620

aac cgt acc gga caa cgt gtt ccc ttc ctg agt gcc gcc aaa atc ggg 1920

Asn Arg Thr Gly Gln Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly

625 630 635 640

cgg gat tat tct ttc ttc aca aac atc gaa acc gac ggt ggt ctg ctg 1968

Arg Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu

645 650 655

gct tcc ctc gac agc gtc gaa aaa aca gcg ggc agt gaa ggc gac acg 2016

Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr

660 665 670

ctg tcc tat tat gtc cgt cgc ggc aat gcg gca cgg act gct tcg gca 2064

Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala

675 680 685

gcg gca cat tcc gcg ccc gcc ggt ctg aaa cac gcc gta gaa cag ggc 2112

Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly

690 695 700

ggc agc aat ctg gaa aac ctg atg gtc gaa ctg gat gcc tcc gaa tca 2160

Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser

705 710 715 720

tcc gca aca ccc gag acg gtt gaa act gcg gcc gcc gac cgc aca gat 2208

Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp

725 730 735

atg ccg ggc atc cgc ccc tac ggc gca act ttc cgc gca gcg gca gcc 2256

Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala

740 745 750

gta cag cat gcg aat gcc gcc gac ggt gta cgc atc ttc aac agt ctc 2304

Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu

755 760 765

gcc gct acc gtc tat gcc gac agt acc gcc gcc cat gcc gat atg cag 2352

Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln

770 775 780

gga cgc cgg ctg aaa gcc gta tcg gac ggg ttg gac cac aac gct acg 2400

Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Ala Thr

785 790 795 800

ggt ctg cgc gtc atc gcg caa acc caa cag gac ggt gga acg tgg gaa 2448

Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu

805 810 815

cag ggc ggt gtt gaa ggc aaa atg cgc ggc agt acc caa acc gtc ggc 2496

Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly

820 825 830

att gcc gcg aaa acc ggc gaa aat acg aca gca gcc gcc aca ctg ggc 2544

Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly

835 840 845

atg gga cac agc aca tgg agc gaa aac agt gca aat gca aaa acc gac 2592

Met Gly His Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp

850 855 860

agc att agt ctg ttt gca ggc ata cgg cac gat gcg ggc gat atc ggc 2640

Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly

865 870 875 880

tat ctc aaa ggc ctg ttc tcc tac gga cgc tac aaa aac agc atc agc 2688

Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser

885 890 895

cgc agc acc ggt gcg gac gaa cat gcg gaa ggc agc gtc aac ggc acg 2736

Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr

900 905 910

ctg atg cag ctg ggc gca ctg ggc ggt gtc aac gtt ccg ttt gcc gca 2784

Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala

915 920 925

acg gga gat ttg acg gtc gaa ggc ggt ctg cgc tac gac ctg ctc aaa 2832

Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys

930 935 940

cag gat gca ttc gcc gaa aaa ggc agt gct ttg ggc tgg agc ggc aac 2880

Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn

945 950 955 960

agc ctc act gaa ggc aca ctg gtc gga ctc gcg ggt ctg aag ctg tcg 2928

Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser

965 970 975

caa ccc ttg agc gat aaa gcc gtc ctg ttt gca acg gcg ggc gtg gaa 2976

Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu

980 985 990

cgc gac ctg aac gga cgc gac tac acg gta acg ggc ggc ttt acc ggc 3024

Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly

995 1000 1005

gcg act gca gca acc ggc aag acg ggg gca cgc aat atg ccg cac acc 3072

Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr

1010 1015 1020

cgc ctg gtt gcc ggt ctg ggc gcg gat gtc gaa ttc ggc aac ggc tgg 3120

Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp

1025 1030 1035 1040

aac ggc ttg gca cgt tac agc tac gcc ggt tcc aaa cag tac ggc aac 3168

Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn

1045 1050 1055

cac agc gga cga gtc ggc gta ggc tac cgg ttc tga 3204

His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe

1060 1065

<210> SEQ ID NO 8

<211> LENGTH: 1067

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 8

Met Arg Thr Thr Pro Thr Phe Pro Thr Lys Thr Phe Lys Pro Ala Ala

1 5 10 15

Met Ala Leu Ala Val Ala Thr Thr Leu Ser Ala Cys Leu Gly Gly Gly

20 25 30

Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly Gly Thr Gly Ile Gly

35 40 45

Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala Ala Val Ser Tyr Ala

50 55 60

Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser Met Leu Cys Ala Gly

65 70 75 80

Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala Lys Ile Asn Ala Pro

85 90 95

Pro Pro Asn Leu His Thr Gly Asp Phe Thr Asn Pro Asn Asp Ala Tyr

100 105 110

Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu Ala Gly Tyr Thr Gly

115 120 125

Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly Glu Ser Val Gly Ser

130 135 140

Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu His Gly Tyr Asn Glu

145 150 155 160

Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys Glu Ala Pro Glu Asp

165 170 175

Gly Gly Gly Lys Asp Ile Lys Ala Ser Phe Asp Asp Glu Ala Val Ile

180 185 190

Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His Val Lys Glu Ile Gly

195 200 205

His Ile Asp Val Val Ser His Ile Ile Gly Gly Arg Ser Val Asp Gly

210 215 220

Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr Leu His Ile Met Asn

225 230 235 240

Thr His Asp Gly Thr Lys Asn Glu Ile Met Ser Ala Ala Ile Arg Asn

245 250 255

Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg Ile Val Asn Asn Ser

260 265 270

Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp His Phe Gln Ile Ala

275 280 285

Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu Ala Tyr Ser Gly Gly

290 295 300

Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln Gln Ser Asp Tyr Gly

305 310 315 320

Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met Leu Phe Ile Phe Ser

325 330 335

Ala Ser Asn Asp Ala Gln Ala Gln Pro Asn Thr Leu Thr Leu Leu Pro

340 345 350

Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile Thr Val Ala Gly Val

355 360 365

Asp Arg Ser Gly Glu Lys Phe Asn Gly Ser Asn His Cys Gly Ile Thr

370 375 380

Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala Ser Val Arg Phe Thr

385 390 395 400

Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser Phe Ser Ala Pro Ile

405 410 415

Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys Tyr Pro Trp Met Ser

420 425 430

Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr Ala Gln Asp Ile Gly

435 440 445

Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly Leu Leu Asp Ala Gly

450 455 460

Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe Gly Asp Phe Thr Ala

465 470 475 480

Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser Phe Arg Asn Asp Ile

485 490 495

Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly Ser Gln Leu Gln Leu

500 505 510

His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile Ile Glu Gly Gly Ser

515 520 525

Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met Arg Val Glu Thr Lys

530 535 540

Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly Gly Ser Leu Asn Ser

545 550 555 560

Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Arg Ser Gly Ala Asn Glu

565 570 575

Thr Val His Ile Lys Gly Asp Leu Gln Leu Gly Gly Glu Gly Thr Leu

580 585 590

Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp Gly Thr Ala Met Thr

595 600 605

Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys Gly Ala Gly Tyr Leu

610 615 620

Asn Arg Thr Gly Gln Arg Val Pro Phe Leu Ser Ala Ala Lys Ile Gly

625 630 635 640

Arg Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr Asp Gly Gly Leu Leu

645 650 655

Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly Ser Glu Gly Asp Thr

660 665 670

Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala Arg Thr Ala Ser Ala

675 680 685

Ala Ala His Ser Ala Pro Ala Gly Leu Lys His Ala Val Glu Gln Gly

690 695 700

Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu Asp Ala Ser Glu Ser

705 710 715 720

Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala Ala Asp Arg Thr Asp

725 730 735

Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe Arg Ala Ala Ala Ala

740 745 750

Val Gln His Ala Asn Ala Ala Asp Gly Val Arg Ile Phe Asn Ser Leu

755 760 765

Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala His Ala Asp Met Gln

770 775 780

Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu Asp His Asn Ala Thr

785 790 795 800

Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp Gly Gly Thr Trp Glu

805 810 815

Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser Thr Gln Thr Val Gly

820 825 830

Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala Ala Ala Thr Leu Gly

835 840 845

Met Gly His Ser Thr Trp Ser Glu Asn Ser Ala Asn Ala Lys Thr Asp

850 855 860

Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp Ala Gly Asp Ile Gly

865 870 875 880

Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr Lys Asn Ser Ile Ser

885 890 895

Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly Ser Val Asn Gly Thr

900 905 910

Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn Val Pro Phe Ala Ala

915 920 925

Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg Tyr Asp Leu Leu Lys

930 935 940

Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu Gly Trp Ser Gly Asn

945 950 955 960

Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala Gly Leu Lys Leu Ser

965 970 975

Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala Thr Ala Gly Val Glu

980 985 990

Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr Gly Gly Phe Thr Gly

995 1000 1005

Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg Asn Met Pro His Thr

1010 1015 1020

Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu Phe Gly Asn Gly Trp

1025 1030 1035 1040

Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser Lys Gln Tyr Gly Asn

1045 1050 1055

His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe

1060 1065

<210> SEQ ID NO 9

<211> LENGTH: 339

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(336)

<400> SEQUENCE: 9

atg aac aca cgc atc atc gtt tcg gct gcg ttc gtt gcg ttg gca tta 48

Met Asn Thr Arg Ile Ile Val Ser Ala Ala Phe Val Ala Leu Ala Leu

1 5 10 15

gca ggt tgc ggc tca atc aat aat gta acc gtt tcc gac cag aaa ctt 96

Ala Gly Cys Gly Ser Ile Asn Asn Val Thr Val Ser Asp Gln Lys Leu

20 25 30

cag gaa cgt gcc gcg ttt gcc ttg ggc gtc agc caa aat gcc gta aaa 144

Gln Glu Arg Ala Ala Phe Ala Leu Gly Val Ser Gln Asn Ala Val Lys

35 40 45

atc agc aac cgc agc aat gaa agc ata cgc atc aac ttt acc gca act 192

Ile Ser Asn Arg Ser Asn Glu Ser Ile Arg Ile Asn Phe Thr Ala Thr

50 55 60

gtg ggt aag cgc gtg agc caa tgc tat gtt acc agt gta atc agc aca 240

Val Gly Lys Arg Val Ser Gln Cys Tyr Val Thr Ser Val Ile Ser Thr

65 70 75 80

atc ggc gtt acc act tcc gat gca att tgt ttg gga ggc gga acg cac 288

Ile Gly Val Thr Thr Ser Asp Ala Ile Cys Leu Gly Gly Gly Thr His

85 90 95

aaa ggc aaa agt caa tgc aat gct ttg ctt aaa gcg gca ggc cgt tgc 336

Lys Gly Lys Ser Gln Cys Asn Ala Leu Leu Lys Ala Ala Gly Arg Cys

100 105 110

taa 339

<210> SEQ ID NO 10

<211> LENGTH: 112

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 10

Met Asn Thr Arg Ile Ile Val Ser Ala Ala Phe Val Ala Leu Ala Leu

1 5 10 15

Ala Gly Cys Gly Ser Ile Asn Asn Val Thr Val Ser Asp Gln Lys Leu

20 25 30

Gln Glu Arg Ala Ala Phe Ala Leu Gly Val Ser Gln Asn Ala Val Lys

35 40 45

Ile Ser Asn Arg Ser Asn Glu Ser Ile Arg Ile Asn Phe Thr Ala Thr

50 55 60

Val Gly Lys Arg Val Ser Gln Cys Tyr Val Thr Ser Val Ile Ser Thr

65 70 75 80

Ile Gly Val Thr Thr Ser Asp Ala Ile Cys Leu Gly Gly Gly Thr His

85 90 95

Lys Gly Lys Ser Gln Cys Asn Ala Leu Leu Lys Ala Ala Gly Arg Cys

100 105 110

<210> SEQ ID NO 11

<211> LENGTH: 1314

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1311)

<400> SEQUENCE: 11

atg ctg acg ttt atc gga ctg ctg att atc ggg gtc atc gta tgg ctg 48

Met Leu Thr Phe Ile Gly Leu Leu Ile Ile Gly Val Ile Val Trp Leu

1 5 10 15

ctg ctg acg gaa aaa gtg tcg ccc atc atc gca tta atc ttg gtg ccg 96

Leu Leu Thr Glu Lys Val Ser Pro Ile Ile Ala Leu Ile Leu Val Pro

20 25 30

ctg ttt ggg gcg ttg ctg gcg ggg ttt gat gta tcc caa tta aaa gaa 144

Leu Phe Gly Ala Leu Leu Ala Gly Phe Asp Val Ser Gln Leu Lys Glu

35 40 45

ttt tat tcg ggc ggc acc aaa tcg gtg atg cag att gtg att atg ttt 192

Phe Tyr Ser Gly Gly Thr Lys Ser Val Met Gln Ile Val Ile Met Phe

50 55 60

atg ttt tcc att ttg ttt ttt gga atc atg aac gat gtg ggg ctg ttc 240

Met Phe Ser Ile Leu Phe Phe Gly Ile Met Asn Asp Val Gly Leu Phe

65 70 75 80

cgt ccg atg ata ggc ggt ttg att aag ctg act cgg ggt aat atc gtg 288

Arg Pro Met Ile Gly Gly Leu Ile Lys Leu Thr Arg Gly Asn Ile Val

85 90 95

gca gtg agt gtg ggg acg gtc ttg gtg tcg gtg gtg gcg cag ttg gac 336

Ala Val Ser Val Gly Thr Val Leu Val Ser Val Val Ala Gln Leu Asp

100 105 110

ggg gcg ggt gcg acg acg ttt tta ttg gtc gtc ccc gcc ctt ttg ccg 384

Gly Ala Gly Ala Thr Thr Phe Leu Leu Val Val Pro Ala Leu Leu Pro

115 120 125

ctt tac aag cgt ctg cat atg aat cct tac ctg ctg ttt ttg ctg ctg 432

Leu Tyr Lys Arg Leu His Met Asn Pro Tyr Leu Leu Phe Leu Leu Leu

130 135 140

act tcc agt gcg gga ttg att aac ctt ctg ccg tgg ggc ggg ccg acc 480

Thr Ser Ser Ala Gly Leu Ile Asn Leu Leu Pro Trp Gly Gly Pro Thr

145 150 155 160

ggg cgg gtt gca agc gtg ttg ggc gca gat gtg ggc gaa ttg tat aaa 528

Gly Arg Val Ala Ser Val Leu Gly Ala Asp Val Gly Glu Leu Tyr Lys

165 170 175

cct ttg ttg acg gtg caa att atc ggt gtg gtg ttt atc ctt gcg ctg 576

Pro Leu Leu Thr Val Gln Ile Ile Gly Val Val Phe Ile Leu Ala Leu

180 185 190

tcc ctg ctt ttg ggt gtg cgt gaa aaa agg cgg att gtc cgg gag ttg 624

Ser Leu Leu Leu Gly Val Arg Glu Lys Arg Arg Ile Val Arg Glu Leu

195 200 205

ggc gcg ttg ccc gcc gtg gcg gat ttg ata aag ccg gtg cct ttg tcg 672

Gly Ala Leu Pro Ala Val Ala Asp Leu Ile Lys Pro Val Pro Leu Ser

210 215 220

gaa gaa gaa caa aaa ttg gcg cgt ccg aaa ctg ttt tgg tgg aat gtc 720

Glu Glu Glu Gln Lys Leu Ala Arg Pro Lys Leu Phe Trp Trp Asn Val

225 230 235 240

ctg ctg ttt ttg gcg gcg atg agc ctg ctt ttt tcg ggc atc ttc ccg 768

Leu Leu Phe Leu Ala Ala Met Ser Leu Leu Phe Ser Gly Ile Phe Pro

245 250 255

ccg ggt tat gta ttt atg ctg gct gca acg gcg gcg ttg ctt ttg aat 816

Pro Gly Tyr Val Phe Met Leu Ala Ala Thr Ala Ala Leu Leu Leu Asn

260 265 270

tac cgc agc ccg cag gaa cag atg gag cgg att tat gcc cac gcc ggc 864

Tyr Arg Ser Pro Gln Glu Gln Met Glu Arg Ile Tyr Ala His Ala Gly

275 280 285

ggc gcg gtg atg atg gcg tcc att att ttg gcg gca ggt acg ttt ttg 912

Gly Ala Val Met Met Ala Ser Ile Ile Leu Ala Ala Gly Thr Phe Leu

290 295 300

ggg att ttg aag ggt gcg ggg atg ttg gac gcg att tcc aaa gac att 960

Gly Ile Leu Lys Gly Ala Gly Met Leu Asp Ala Ile Ser Lys Asp Ile

305 310 315 320

gtg cat atc ctg ccg gac gcg ctg ctg cct tat ctg cat att gcc atc 1008

Val His Ile Leu Pro Asp Ala Leu Leu Pro Tyr Leu His Ile Ala Ile

325 330 335

ggt gtg ttg ggc att ccg ctt gag ttg gtt ttg agt acg gac gct tat 1056

Gly Val Leu Gly Ile Pro Leu Glu Leu Val Leu Ser Thr Asp Ala Tyr

340 345 350

tat ttc gga ctg ttt ccg att gtg gag cag att acc tcg cag gcg ggc 1104

Tyr Phe Gly Leu Phe Pro Ile Val Glu Gln Ile Thr Ser Gln Ala Gly

355 360 365

gtg gcg ccc gaa gca gca ggt tat gcg atg ttg atc ggc agt atc gtc 1152

Val Ala Pro Glu Ala Ala Gly Tyr Ala Met Leu Ile Gly Ser Ile Val

370 375 380

ggc act ttt gtt acg ccg ctt tcg ccg gct ttg tgg atg ggc ttg ggt 1200

Gly Thr Phe Val Thr Pro Leu Ser Pro Ala Leu Trp Met Gly Leu Gly

385 390 395 400

ttg gcg aaa ttg tcg atg ggc aaa cac atc cgt tat tcg ttt ttt tgg 1248

Leu Ala Lys Leu Ser Met Gly Lys His Ile Arg Tyr Ser Phe Phe Trp

405 410 415

gcg tgg ggt ttg tcg ctg gcg ata ttg gcc agt tcg ata gcg gca gga 1296

Ala Trp Gly Leu Ser Leu Ala Ile Leu Ala Ser Ser Ile Ala Ala Gly

420 425 430

atc gtg cct ctg ccg taa 1314

Ile Val Pro Leu Pro

435

<210> SEQ ID NO 12

<211> LENGTH: 437

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 12

Met Leu Thr Phe Ile Gly Leu Leu Ile Ile Gly Val Ile Val Trp Leu

1 5 10 15

Leu Leu Thr Glu Lys Val Ser Pro Ile Ile Ala Leu Ile Leu Val Pro

20 25 30

Leu Phe Gly Ala Leu Leu Ala Gly Phe Asp Val Ser Gln Leu Lys Glu

35 40 45

Phe Tyr Ser Gly Gly Thr Lys Ser Val Met Gln Ile Val Ile Met Phe

50 55 60

Met Phe Ser Ile Leu Phe Phe Gly Ile Met Asn Asp Val Gly Leu Phe

65 70 75 80

Arg Pro Met Ile Gly Gly Leu Ile Lys Leu Thr Arg Gly Asn Ile Val

85 90 95

Ala Val Ser Val Gly Thr Val Leu Val Ser Val Val Ala Gln Leu Asp

100 105 110

Gly Ala Gly Ala Thr Thr Phe Leu Leu Val Val Pro Ala Leu Leu Pro

115 120 125

Leu Tyr Lys Arg Leu His Met Asn Pro Tyr Leu Leu Phe Leu Leu Leu

130 135 140

Thr Ser Ser Ala Gly Leu Ile Asn Leu Leu Pro Trp Gly Gly Pro Thr

145 150 155 160

Gly Arg Val Ala Ser Val Leu Gly Ala Asp Val Gly Glu Leu Tyr Lys

165 170 175

Pro Leu Leu Thr Val Gln Ile Ile Gly Val Val Phe Ile Leu Ala Leu

180 185 190

Ser Leu Leu Leu Gly Val Arg Glu Lys Arg Arg Ile Val Arg Glu Leu

195 200 205

Gly Ala Leu Pro Ala Val Ala Asp Leu Ile Lys Pro Val Pro Leu Ser

210 215 220

Glu Glu Glu Gln Lys Leu Ala Arg Pro Lys Leu Phe Trp Trp Asn Val

225 230 235 240

Leu Leu Phe Leu Ala Ala Met Ser Leu Leu Phe Ser Gly Ile Phe Pro

245 250 255

Pro Gly Tyr Val Phe Met Leu Ala Ala Thr Ala Ala Leu Leu Leu Asn

260 265 270

Tyr Arg Ser Pro Gln Glu Gln Met Glu Arg Ile Tyr Ala His Ala Gly

275 280 285

Gly Ala Val Met Met Ala Ser Ile Ile Leu Ala Ala Gly Thr Phe Leu

290 295 300

Gly Ile Leu Lys Gly Ala Gly Met Leu Asp Ala Ile Ser Lys Asp Ile

305 310 315 320

Val His Ile Leu Pro Asp Ala Leu Leu Pro Tyr Leu His Ile Ala Ile

325 330 335

Gly Val Leu Gly Ile Pro Leu Glu Leu Val Leu Ser Thr Asp Ala Tyr

340 345 350

Tyr Phe Gly Leu Phe Pro Ile Val Glu Gln Ile Thr Ser Gln Ala Gly

355 360 365

Val Ala Pro Glu Ala Ala Gly Tyr Ala Met Leu Ile Gly Ser Ile Val

370 375 380

Gly Thr Phe Val Thr Pro Leu Ser Pro Ala Leu Trp Met Gly Leu Gly

385 390 395 400

Leu Ala Lys Leu Ser Met Gly Lys His Ile Arg Tyr Ser Phe Phe Trp

405 410 415

Ala Trp Gly Leu Ser Leu Ala Ile Leu Ala Ser Ser Ile Ala Ala Gly

420 425 430

Ile Val Pro Leu Pro

435

<210> SEQ ID NO 13

<211> LENGTH: 1155

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1152)

<400> SEQUENCE: 13

atg ggc atc cat ctc gac ttc ggc att agt cct aaa acg ttc cga cag 48

Met Gly Ile His Leu Asp Phe Gly Ile Ser Pro Lys Thr Phe Arg Gln

1 5 10 15

act tat ctg tat caa aag ccc aag ctc ttt aaa gga gcg gtt cgg aat 96

Thr Tyr Leu Tyr Gln Lys Pro Lys Leu Phe Lys Gly Ala Val Arg Asn

20 25 30

ctc gaa gcc gca tct tgt aaa tat atc aac gag ata tac caa cga gca 144

Leu Glu Ala Ala Ser Cys Lys Tyr Ile Asn Glu Ile Tyr Gln Arg Ala

35 40 45

gac cca acc gca ccg ctg ttt cat ctg cgt aaa aaa ggc gca atc gtt 192

Asp Pro Thr Ala Pro Leu Phe His Leu Arg Lys Lys Gly Ala Ile Val

50 55 60

cct aaa gaa gaa tac gtc gaa agt ttc gac gat ttg ggc aaa act cgc 240

Pro Lys Glu Glu Tyr Val Glu Ser Phe Asp Asp Leu Gly Lys Thr Arg

65 70 75 80

tac cgt ttt att aaa tcc gtt atc tac gaa cat atg aag aat ggt gcg 288

Tyr Arg Phe Ile Lys Ser Val Ile Tyr Glu His Met Lys Asn Gly Ala

85 90 95

tcg tta gtc tat aac cat att aac aac gag ccg ttt tca gac cat atc 336

Ser Leu Val Tyr Asn His Ile Asn Asn Glu Pro Phe Ser Asp His Ile

100 105 110

gcc cgt caa gtc gcc cgc ttt gcc ggc gca cat act att gtt agt gga 384

Ala Arg Gln Val Ala Arg Phe Ala Gly Ala His Thr Ile Val Ser Gly

115 120 125

tat ctt gct ttt ggc agc gac gaa tct tat aaa aac cat tgg gat acc 432

Tyr Leu Ala Phe Gly Ser Asp Glu Ser Tyr Lys Asn His Trp Asp Thr

130 135 140

cgc gat gtg tat gcc atc cag ctt ttc ggc aag aaa cgt tgg caa ctt 480

Arg Asp Val Tyr Ala Ile Gln Leu Phe Gly Lys Lys Arg Trp Gln Leu

145 150 155 160

act gcc cct gat ttc cct atg cca ttg tat atg caa cag act aaa gat 528

Thr Ala Pro Asp Phe Pro Met Pro Leu Tyr Met Gln Gln Thr Lys Asp

165 170 175

act gat att tcc att cct gaa cat atc gat atg gat att atc ctt gaa 576

Thr Asp Ile Ser Ile Pro Glu His Ile Asp Met Asp Ile Ile Leu Glu

180 185 190

gca ggt gat gtc ctc tac atc cca cgc ggt tgg tgg cac aga cct atc 624

Ala Gly Asp Val Leu Tyr Ile Pro Arg Gly Trp Trp His Arg Pro Ile

195 200 205

ccg ctc ggc tgt gaa acc ttc cac ttc gct gtc ggt acc ttc ccg ccc 672

Pro Leu Gly Cys Glu Thr Phe His Phe Ala Val Gly Thr Phe Pro Pro

210 215 220

aac ggc tat aat tac ctc gag tgg cta atg aag aaa ttc ccc acg ata 720

Asn Gly Tyr Asn Tyr Leu Glu Trp Leu Met Lys Lys Phe Pro Thr Ile

225 230 235 240

gaa agt ctg cgc cac agt ttc tca gac tgg gag caa gat agg acg cgt 768

Glu Ser Leu Arg His Ser Phe Ser Asp Trp Glu Gln Asp Arg Thr Arg

245 250 255

atc aac gat act gcc gca caa att gct gcc atg att gcc gac ccc gtc 816

Ile Asn Asp Thr Ala Ala Gln Ile Ala Ala Met Ile Ala Asp Pro Val

260 265 270

aat tac gaa gcc ttc agt gaa gac ttc ctc ggc aaa gaa cgc acc gat 864

Asn Tyr Glu Ala Phe Ser Glu Asp Phe Leu Gly Lys Glu Arg Thr Asp

275 280 285

acc gct ttt cat ctc gaa cag ttc gcg aat ccc aac gct act ccg ctt 912

Thr Ala Phe His Leu Glu Gln Phe Ala Asn Pro Asn Ala Thr Pro Leu

290 295 300

tca gac gac gtc agg ttg aga cta aat gcc aat aat ttg gat acg ttg 960

Ser Asp Asp Val Arg Leu Arg Leu Asn Ala Asn Asn Leu Asp Thr Leu

305 310 315 320

gaa aag gga tat ttg att ggg aat ggg atg aag ata agc gta gat gaa 1008

Glu Lys Gly Tyr Leu Ile Gly Asn Gly Met Lys Ile Ser Val Asp Glu

325 330 335

ttg ggg aaa aaa gtg tta gaa cac atc ggt aag aat gaa ccg tta ttg 1056

Leu Gly Lys Lys Val Leu Glu His Ile Gly Lys Asn Glu Pro Leu Leu

340 345 350

ttg aaa aat cta ctg gtt aac ttc aat cag gga aaa cat gaa gaa gtt 1104

Leu Lys Asn Leu Leu Val Asn Phe Asn Gln Gly Lys His Glu Glu Val

355 360 365

agg aag ttg att tat cag ttg ata gag tta gat ttt ctg gaa ctt ttg 1152

Arg Lys Leu Ile Tyr Gln Leu Ile Glu Leu Asp Phe Leu Glu Leu Leu

370 375 380

tga 1155

<210> SEQ ID NO 14

<211> LENGTH: 384

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 14

Met Gly Ile His Leu Asp Phe Gly Ile Ser Pro Lys Thr Phe Arg Gln

1 5 10 15

Thr Tyr Leu Tyr Gln Lys Pro Lys Leu Phe Lys Gly Ala Val Arg Asn

20 25 30

Leu Glu Ala Ala Ser Cys Lys Tyr Ile Asn Glu Ile Tyr Gln Arg Ala

35 40 45

Asp Pro Thr Ala Pro Leu Phe His Leu Arg Lys Lys Gly Ala Ile Val

50 55 60

Pro Lys Glu Glu Tyr Val Glu Ser Phe Asp Asp Leu Gly Lys Thr Arg

65 70 75 80

Tyr Arg Phe Ile Lys Ser Val Ile Tyr Glu His Met Lys Asn Gly Ala

85 90 95

Ser Leu Val Tyr Asn His Ile Asn Asn Glu Pro Phe Ser Asp His Ile

100 105 110

Ala Arg Gln Val Ala Arg Phe Ala Gly Ala His Thr Ile Val Ser Gly

115 120 125

Tyr Leu Ala Phe Gly Ser Asp Glu Ser Tyr Lys Asn His Trp Asp Thr

130 135 140

Arg Asp Val Tyr Ala Ile Gln Leu Phe Gly Lys Lys Arg Trp Gln Leu

145 150 155 160

Thr Ala Pro Asp Phe Pro Met Pro Leu Tyr Met Gln Gln Thr Lys Asp

165 170 175

Thr Asp Ile Ser Ile Pro Glu His Ile Asp Met Asp Ile Ile Leu Glu

180 185 190

Ala Gly Asp Val Leu Tyr Ile Pro Arg Gly Trp Trp His Arg Pro Ile

195 200 205

Pro Leu Gly Cys Glu Thr Phe His Phe Ala Val Gly Thr Phe Pro Pro

210 215 220

Asn Gly Tyr Asn Tyr Leu Glu Trp Leu Met Lys Lys Phe Pro Thr Ile

225 230 235 240

Glu Ser Leu Arg His Ser Phe Ser Asp Trp Glu Gln Asp Arg Thr Arg

245 250 255

Ile Asn Asp Thr Ala Ala Gln Ile Ala Ala Met Ile Ala Asp Pro Val

260 265 270

Asn Tyr Glu Ala Phe Ser Glu Asp Phe Leu Gly Lys Glu Arg Thr Asp

275 280 285

Thr Ala Phe His Leu Glu Gln Phe Ala Asn Pro Asn Ala Thr Pro Leu

290 295 300

Ser Asp Asp Val Arg Leu Arg Leu Asn Ala Asn Asn Leu Asp Thr Leu

305 310 315 320

Glu Lys Gly Tyr Leu Ile Gly Asn Gly Met Lys Ile Ser Val Asp Glu

325 330 335

Leu Gly Lys Lys Val Leu Glu His Ile Gly Lys Asn Glu Pro Leu Leu

340 345 350

Leu Lys Asn Leu Leu Val Asn Phe Asn Gln Gly Lys His Glu Glu Val

355 360 365

Arg Lys Leu Ile Tyr Gln Leu Ile Glu Leu Asp Phe Leu Glu Leu Leu

370 375 380

<210> SEQ ID NO 15

<211> LENGTH: 717

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(714)

<400> SEQUENCE: 15

atg aat aga ccc aag caa ccc ttc ttc cgt ccc gaa gtc gcc gtt gcc 48

Met Asn Arg Pro Lys Gln Pro Phe Phe Arg Pro Glu Val Ala Val Ala

1 5 10 15

cgc caa acc agc ctg acg ggt aaa gtg att ctg aca cga ccg ttg tca 96

Arg Gln Thr Ser Leu Thr Gly Lys Val Ile Leu Thr Arg Pro Leu Ser

20 25 30

ttt tcc cta tgg acg aca ttt gca tcg ata tct gcg tta ttg att atc 144

Phe Ser Leu Trp Thr Thr Phe Ala Ser Ile Ser Ala Leu Leu Ile Ile

35 40 45

ctg ttt ttg ata ttt ggt aac tat acg cga aag aca aca gtg gag gga 192

Leu Phe Leu Ile Phe Gly Asn Tyr Thr Arg Lys Thr Thr Val Glu Gly

50 55 60

caa att tta cct gca tcg ggc gta atc agg gtg tat gca ccg gat acg 240

Gln Ile Leu Pro Ala Ser Gly Val Ile Arg Val Tyr Ala Pro Asp Thr

65 70 75 80

ggg aca att aca gcg aaa ttc gtg gaa gat gga gaa aag gtt aag gct 288

Gly Thr Ile Thr Ala Lys Phe Val Glu Asp Gly Glu Lys Val Lys Ala

85 90 95

ggc gac aag cta ttt gcg ctt tcg acc tca cgt ttc ggc gca gga gat 336

Gly Asp Lys Leu Phe Ala Leu Ser Thr Ser Arg Phe Gly Ala Gly Asp

100 105 110

agc gtg cag cag cag ttg aaa acg gag gca gtt ttg aag aaa acg ttg 384

Ser Val Gln Gln Gln Leu Lys Thr Glu Ala Val Leu Lys Lys Thr Leu

115 120 125

gca gaa cag gaa ctg ggt cgt ctg aag ctg ata cac ggg aat gaa acg 432

Ala Glu Gln Glu Leu Gly Arg Leu Lys Leu Ile His Gly Asn Glu Thr

130 135 140

cgc agc ctt aaa gca act gtc gaa cgt ttg gaa aac cag aaa ctc cat 480

Arg Ser Leu Lys Ala Thr Val Glu Arg Leu Glu Asn Gln Lys Leu His

145 150 155 160

att tcg caa cag ata gac ggt cag aaa agg cgc att aga ctt gcg gaa 528

Ile Ser Gln Gln Ile Asp Gly Gln Lys Arg Arg Ile Arg Leu Ala Glu

165 170 175

gaa atg ttg cag aaa tat cgt ttc cta tcc gcc aat gat gca gtg cca 576

Glu Met Leu Gln Lys Tyr Arg Phe Leu Ser Ala Asn Asp Ala Val Pro

180 185 190

aaa caa gaa atg atg aat gtc aag gca gag ctt tta gag cag aaa gcc 624

Lys Gln Glu Met Met Asn Val Lys Ala Glu Leu Leu Glu Gln Lys Ala

195 200 205

aaa ctt gat gcc tac cgc cga gaa gaa gtc ggg ctg ctt cag gaa atc 672

Lys Leu Asp Ala Tyr Arg Arg Glu Glu Val Gly Leu Leu Gln Glu Ile

210 215 220

cgc acg cag aat ctg aca ttg gcc agc ctc ccc caa gcg gca tga 717

Arg Thr Gln Asn Leu Thr Leu Ala Ser Leu Pro Gln Ala Ala

225 230 235

<210> SEQ ID NO 16

<211> LENGTH: 238

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 16

Met Asn Arg Pro Lys Gln Pro Phe Phe Arg Pro Glu Val Ala Val Ala

1 5 10 15

Arg Gln Thr Ser Leu Thr Gly Lys Val Ile Leu Thr Arg Pro Leu Ser

20 25 30

Phe Ser Leu Trp Thr Thr Phe Ala Ser Ile Ser Ala Leu Leu Ile Ile

35 40 45

Leu Phe Leu Ile Phe Gly Asn Tyr Thr Arg Lys Thr Thr Val Glu Gly

50 55 60

Gln Ile Leu Pro Ala Ser Gly Val Ile Arg Val Tyr Ala Pro Asp Thr

65 70 75 80

Gly Thr Ile Thr Ala Lys Phe Val Glu Asp Gly Glu Lys Val Lys Ala

85 90 95

Gly Asp Lys Leu Phe Ala Leu Ser Thr Ser Arg Phe Gly Ala Gly Asp

100 105 110

Ser Val Gln Gln Gln Leu Lys Thr Glu Ala Val Leu Lys Lys Thr Leu

115 120 125

Ala Glu Gln Glu Leu Gly Arg Leu Lys Leu Ile His Gly Asn Glu Thr

130 135 140

Arg Ser Leu Lys Ala Thr Val Glu Arg Leu Glu Asn Gln Lys Leu His

145 150 155 160

Ile Ser Gln Gln Ile Asp Gly Gln Lys Arg Arg Ile Arg Leu Ala Glu

165 170 175

Glu Met Leu Gln Lys Tyr Arg Phe Leu Ser Ala Asn Asp Ala Val Pro

180 185 190

Lys Gln Glu Met Met Asn Val Lys Ala Glu Leu Leu Glu Gln Lys Ala

195 200 205

Lys Leu Asp Ala Tyr Arg Arg Glu Glu Val Gly Leu Leu Gln Glu Ile

210 215 220

Arg Thr Gln Asn Leu Thr Leu Ala Ser Leu Pro Gln Ala Ala

225 230 235

<210> SEQ ID NO 17

<211> LENGTH: 690

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(687)

<400> SEQUENCE: 17

atg atg aat gtc gag gca gag ctt tta gag cag aaa gcc aaa ctt gat 48

Met Met Asn Val Glu Ala Glu Leu Leu Glu Gln Lys Ala Lys Leu Asp

1 5 10 15

gcc tac ggc cga gaa gaa gcc ggg ctg ctt cag gaa atc cgc acg cag 96

Ala Tyr Gly Arg Glu Glu Ala Gly Leu Leu Gln Glu Ile Arg Thr Gln

20 25 30

aat ctg aca ttg gcc agc ctc ccc aaa cgg cat gag aca gaa caa agc 144

Asn Leu Thr Leu Ala Ser Leu Pro Lys Arg His Glu Thr Glu Gln Ser

35 40 45

cag ctt gaa cgc acc atg gcc gat att tct caa gaa gtt ttg gat ttt 192

Gln Leu Glu Arg Thr Met Ala Asp Ile Ser Gln Glu Val Leu Asp Phe

50 55 60

gaa atg cgc tct gaa caa atc atc cgt gca gga cgg tcg ggt tat ata 240

Glu Met Arg Ser Glu Gln Ile Ile Arg Ala Gly Arg Ser Gly Tyr Ile

65 70 75 80

gca ata ccg aac gtc gaa gtc gga cag cag gtt gat cct tcc aaa ctg 288

Ala Ile Pro Asn Val Glu Val Gly Gln Gln Val Asp Pro Ser Lys Leu

85 90 95

ctc ttg agc att gtt ccc gaa cgt acc gag cta tat gcc cat cta tat 336

Leu Leu Ser Ile Val Pro Glu Arg Thr Glu Leu Tyr Ala His Leu Tyr

100 105 110

atc ccc agc agt gca gca ggc ttt atc aag ccg aaa gac aag gtt gtc 384

Ile Pro Ser Ser Ala Ala Gly Phe Ile Lys Pro Lys Asp Lys Val Val

115 120 125

cta cgt tat cag gca tat ccc tat caa aaa ttc ggg ctt gct tcc ggc 432

Leu Arg Tyr Gln Ala Tyr Pro Tyr Gln Lys Phe Gly Leu Ala Ser Gly

130 135 140

agt gtc gta tca gta gca aaa acg gca ctg ggc aga cag gaa ttg tcg 480

Ser Val Val Ser Val Ala Lys Thr Ala Leu Gly Arg Gln Glu Leu Ser

145 150 155 160

gga ttg ggc atg gta tcc tcc gat ttg gcg aag agc aac gaa cct gtt 528

Gly Leu Gly Met Val Ser Ser Asp Leu Ala Lys Ser Asn Glu Pro Val

165 170 175

tat ctc gtg aaa ata aaa ccc gac aaa cca acc atc act gca tac ggt 576

Tyr Leu Val Lys Ile Lys Pro Asp Lys Pro Thr Ile Thr Ala Tyr Gly

180 185 190

gag gaa aaa ccg ctg caa atc ggc atg acg ttg gaa gca gac atc ctg 624

Glu Glu Lys Pro Leu Gln Ile Gly Met Thr Leu Glu Ala Asp Ile Leu

195 200 205

cac gag aaa cgg cgg ctg tac gaa tgg gta ttg gag ctg att tat agt 672

His Glu Lys Arg Arg Leu Tyr Glu Trp Val Leu Glu Leu Ile Tyr Ser

210 215 220

atg tcg ggc aaa ctg taa 690

Met Ser Gly Lys Leu

225

<210> SEQ ID NO 18

<211> LENGTH: 229

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 18

Met Met Asn Val Glu Ala Glu Leu Leu Glu Gln Lys Ala Lys Leu Asp

1 5 10 15

Ala Tyr Gly Arg Glu Glu Ala Gly Leu Leu Gln Glu Ile Arg Thr Gln

20 25 30

Asn Leu Thr Leu Ala Ser Leu Pro Lys Arg His Glu Thr Glu Gln Ser

35 40 45

Gln Leu Glu Arg Thr Met Ala Asp Ile Ser Gln Glu Val Leu Asp Phe

50 55 60

Glu Met Arg Ser Glu Gln Ile Ile Arg Ala Gly Arg Ser Gly Tyr Ile

65 70 75 80

Ala Ile Pro Asn Val Glu Val Gly Gln Gln Val Asp Pro Ser Lys Leu

85 90 95

Leu Leu Ser Ile Val Pro Glu Arg Thr Glu Leu Tyr Ala His Leu Tyr

100 105 110

Ile Pro Ser Ser Ala Ala Gly Phe Ile Lys Pro Lys Asp Lys Val Val

115 120 125

Leu Arg Tyr Gln Ala Tyr Pro Tyr Gln Lys Phe Gly Leu Ala Ser Gly

130 135 140

Ser Val Val Ser Val Ala Lys Thr Ala Leu Gly Arg Gln Glu Leu Ser

145 150 155 160

Gly Leu Gly Met Val Ser Ser Asp Leu Ala Lys Ser Asn Glu Pro Val

165 170 175

Tyr Leu Val Lys Ile Lys Pro Asp Lys Pro Thr Ile Thr Ala Tyr Gly

180 185 190

Glu Glu Lys Pro Leu Gln Ile Gly Met Thr Leu Glu Ala Asp Ile Leu

195 200 205

His Glu Lys Arg Arg Leu Tyr Glu Trp Val Leu Glu Leu Ile Tyr Ser

210 215 220

Met Ser Gly Lys Leu

225

<210> SEQ ID NO 19

<211> LENGTH: 1743

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1740)

<400> SEQUENCE: 19

atg aaa ttt ttt cct gct cca tgt ctg ttg gtt atc ctg gct gtc ata 48

Met Lys Phe Phe Pro Ala Pro Cys Leu Leu Val Ile Leu Ala Val Ile

1 5 10 15

ccc ctt aaa acc tta gct gcc gat gaa aac gat gca gaa ctt atc cgt 96

Pro Leu Lys Thr Leu Ala Ala Asp Glu Asn Asp Ala Glu Leu Ile Arg

20 25 30

tcc atg cag cgt cag cag cac ata gat gct gaa ttg tta act gat gca 144

Ser Met Gln Arg Gln Gln His Ile Asp Ala Glu Leu Leu Thr Asp Ala

35 40 45

aat gtc cgt ttc gag caa cca ttg gag aag aac aat tat gtc ctg agt 192

Asn Val Arg Phe Glu Gln Pro Leu Glu Lys Asn Asn Tyr Val Leu Ser

50 55 60

gaa gat gaa aca ccg tgt act cgg gta aat tac att agt tta gat gat 240

Glu Asp Glu Thr Pro Cys Thr Arg Val Asn Tyr Ile Ser Leu Asp Asp

65 70 75 80

aag acg gcg cgc aaa ttt tct ttt ctt cct tct gtg ctc atg aaa gaa 288

Lys Thr Ala Arg Lys Phe Ser Phe Leu Pro Ser Val Leu Met Lys Glu

85 90 95

aca gct ttt aaa act ggg atg tgt tta ggt tcc aat aat ttg agc agg 336

Thr Ala Phe Lys Thr Gly Met Cys Leu Gly Ser Asn Asn Leu Ser Arg

100 105 110

cta caa aaa gcc gcg caa cag ata ctg att gtg cgt ggc tac ctc act 384

Leu Gln Lys Ala Ala Gln Gln Ile Leu Ile Val Arg Gly Tyr Leu Thr

115 120 125

tcc caa gct att atc caa cca cag aat atg gat tcg gga att ctg aaa 432

Ser Gln Ala Ile Ile Gln Pro Gln Asn Met Asp Ser Gly Ile Leu Lys

130 135 140

tta cgg gta tca gca ggc gaa atc agg gat atc cgc tat gaa gaa aaa 480

Leu Arg Val Ser Ala Gly Glu Ile Arg Asp Ile Arg Tyr Glu Glu Lys

145 150 155 160

cgg gat gcg aag tct gcc gag ggc agt att agt gca ttc aat aac aaa 528

Arg Asp Ala Lys Ser Ala Glu Gly Ser Ile Ser Ala Phe Asn Asn Lys

165 170 175

ctt ccc tta tat agg aac aaa att ctc aat ctt cgc gat gta gag cag 576

Leu Pro Leu Tyr Arg Asn Lys Ile Leu Asn Leu Arg Asp Val Glu Gln

180 185 190

ggc ttg gaa aac ctg cgt cgt ttg ccg agt gtt aaa aca gat att cag 624

Gly Leu Glu Asn Leu Arg Arg Leu Pro Ser Val Lys Thr Asp Ile Gln

195 200 205

att ata ccg tcc gaa gaa gaa ggc aaa agc gat tta cag atc aaa tgg 672

Ile Ile Pro Ser Glu Glu Glu Gly Lys Ser Asp Leu Gln Ile Lys Trp

210 215 220

cag cag aat aaa ccc ata cgg ttc agt atc ggt ata gat gat gcg ggc 720

Gln Gln Asn Lys Pro Ile Arg Phe Ser Ile Gly Ile Asp Asp Ala Gly

225 230 235 240

ggc aaa acg acc ggc aaa tat caa gga aat gtc gct tta tcg tcc gat 768

Gly Lys Thr Thr Gly Lys Tyr Gln Gly Asn Val Ala Leu Ser Ser Asp

245 250 255

aac cct ttg ggc tta agc gat tcg ttt tat gtt tca tat gga cgc ggt 816

Asn Pro Leu Gly Leu Ser Asp Ser Phe Tyr Val Ser Tyr Gly Arg Gly

260 265 270

ttg gtg cac aaa acg gac ttg act gct gcc acc ggt acg gaa act gaa 864

Leu Val His Lys Thr Asp Leu Thr Ala Ala Thr Gly Thr Glu Thr Glu

275 280 285

agc gga tcc aga agt tac agc gtg cat tat tcg gtg ccc gta aaa aaa 912

Ser Gly Ser Arg Ser Tyr Ser Val His Tyr Ser Val Pro Val Lys Lys

290 295 300

tgg ctg ttt tct ttt aat cac aat gga cat cgt tac cac gaa gca acc 960

Trp Leu Phe Ser Phe Asn His Asn Gly His Arg Tyr His Glu Ala Thr

305 310 315 320

gaa ggc tat tcc gtc aat tac gat tac aac ggc aaa caa tat cag agc 1008

Glu Gly Tyr Ser Val Asn Tyr Asp Tyr Asn Gly Lys Gln Tyr Gln Ser

325 330 335

agc ctg gcc gcc gag cgc atg ctt tgg ccc ccc agc ttt cct caa act 1056

Ser Leu Ala Ala Glu Arg Met Leu Trp Pro Pro Ser Phe Pro Gln Thr

340 345 350

tca gtc cga atg aaa tta tgg aca cgc caa acc tat aaa tac atc gac 1104

Ser Val Arg Met Lys Leu Trp Thr Arg Gln Thr Tyr Lys Tyr Ile Asp

355 360 365

gat gcc gaa atc gaa gtg caa cgc cgc cgc tct gca ggc tgg gaa gcc 1152

Asp Ala Glu Ile Glu Val Gln Arg Arg Arg Ser Ala Gly Trp Glu Ala

370 375 380

gaa ttg cgc cac cgt gct tac ctc cac cgt tgg cag ctt gac ggc aag 1200

Glu Leu Arg His Arg Ala Tyr Leu His Arg Trp Gln Leu Asp Gly Lys

385 390 395 400

ttg tct tac aaa cgc ggg acc ggc atg cgc caa agt atg ccc gca cct 1248

Leu Ser Tyr Lys Arg Gly Thr Gly Met Arg Gln Ser Met Pro Ala Pro

405 410 415

gaa gaa aac ggc ggc ggt act att cca gcc aca tcc cgt atg aaa atc 1296

Glu Glu Asn Gly Gly Gly Thr Ile Pro Ala Thr Ser Arg Met Lys Ile

420 425 430

ata acc gcc gga ttg gat gca gcg gcc ccg tct atg ttg ggc aaa cag 1344

Ile Thr Ala Gly Leu Asp Ala Ala Ala Pro Ser Met Leu Gly Lys Gln

435 440 445

cag ttt ttc tac gca acc gcc att caa gct caa tgg aac aaa acg cct 1392

Gln Phe Phe Tyr Ala Thr Ala Ile Gln Ala Gln Trp Asn Lys Thr Pro

450 455 460

ttg gtt gcc caa gac aag ttg tct atc ggc agc cgc tac acc gtt cgc 1440

Leu Val Ala Gln Asp Lys Leu Ser Ile Gly Ser Arg Tyr Thr Val Arg

465 470 475 480

gga ttt gat ggg gag cag agt ctt ttc gga gag cga ggt ttc tac tgg 1488

Gly Phe Asp Gly Glu Gln Ser Leu Phe Gly Glu Arg Gly Phe Tyr Trp

485 490 495

cag aat act tta act tgg tat ttt cat ccg aac cat cag ttc tat ctc 1536

Gln Asn Thr Leu Thr Trp Tyr Phe His Pro Asn His Gln Phe Tyr Leu

500 505 510

ggt gcg gac tat ggc cgc gta tct ggc gaa agt gca caa tat gta tcg 1584

Gly Ala Asp Tyr Gly Arg Val Ser Gly Glu Ser Ala Gln Tyr Val Ser

515 520 525

ggc aag cag ctg atg ggt gca gtg gtc ggc ttc aga gga ggg cat aaa 1632

Gly Lys Gln Leu Met Gly Ala Val Val Gly Phe Arg Gly Gly His Lys

530 535 540

gta ggc ggt atg ttt gct tat gat ctg ttt gcc ggc aag ccg ctt cat 1680

Val Gly Gly Met Phe Ala Tyr Asp Leu Phe Ala Gly Lys Pro Leu His

545 550 555 560

aaa ccc aaa ggc ttt cag acg acc aac acc gtt tac ggc ttc aac ttg 1728

Lys Pro Lys Gly Phe Gln Thr Thr Asn Thr Val Tyr Gly Phe Asn Leu

565 570 575

aat tac agt ttc taa 1743

Asn Tyr Ser Phe

580

<210> SEQ ID NO 20

<211> LENGTH: 580

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 20

Met Lys Phe Phe Pro Ala Pro Cys Leu Leu Val Ile Leu Ala Val Ile

1 5 10 15

Pro Leu Lys Thr Leu Ala Ala Asp Glu Asn Asp Ala Glu Leu Ile Arg

20 25 30

Ser Met Gln Arg Gln Gln His Ile Asp Ala Glu Leu Leu Thr Asp Ala

35 40 45

Asn Val Arg Phe Glu Gln Pro Leu Glu Lys Asn Asn Tyr Val Leu Ser

50 55 60

Glu Asp Glu Thr Pro Cys Thr Arg Val Asn Tyr Ile Ser Leu Asp Asp

65 70 75 80

Lys Thr Ala Arg Lys Phe Ser Phe Leu Pro Ser Val Leu Met Lys Glu

85 90 95

Thr Ala Phe Lys Thr Gly Met Cys Leu Gly Ser Asn Asn Leu Ser Arg

100 105 110

Leu Gln Lys Ala Ala Gln Gln Ile Leu Ile Val Arg Gly Tyr Leu Thr

115 120 125

Ser Gln Ala Ile Ile Gln Pro Gln Asn Met Asp Ser Gly Ile Leu Lys

130 135 140

Leu Arg Val Ser Ala Gly Glu Ile Arg Asp Ile Arg Tyr Glu Glu Lys

145 150 155 160

Arg Asp Ala Lys Ser Ala Glu Gly Ser Ile Ser Ala Phe Asn Asn Lys

165 170 175

Leu Pro Leu Tyr Arg Asn Lys Ile Leu Asn Leu Arg Asp Val Glu Gln

180 185 190

Gly Leu Glu Asn Leu Arg Arg Leu Pro Ser Val Lys Thr Asp Ile Gln

195 200 205

Ile Ile Pro Ser Glu Glu Glu Gly Lys Ser Asp Leu Gln Ile Lys Trp

210 215 220

Gln Gln Asn Lys Pro Ile Arg Phe Ser Ile Gly Ile Asp Asp Ala Gly

225 230 235 240

Gly Lys Thr Thr Gly Lys Tyr Gln Gly Asn Val Ala Leu Ser Ser Asp

245 250 255

Asn Pro Leu Gly Leu Ser Asp Ser Phe Tyr Val Ser Tyr Gly Arg Gly

260 265 270

Leu Val His Lys Thr Asp Leu Thr Ala Ala Thr Gly Thr Glu Thr Glu

275 280 285

Ser Gly Ser Arg Ser Tyr Ser Val His Tyr Ser Val Pro Val Lys Lys

290 295 300

Trp Leu Phe Ser Phe Asn His Asn Gly His Arg Tyr His Glu Ala Thr

305 310 315 320

Glu Gly Tyr Ser Val Asn Tyr Asp Tyr Asn Gly Lys Gln Tyr Gln Ser

325 330 335

Ser Leu Ala Ala Glu Arg Met Leu Trp Pro Pro Ser Phe Pro Gln Thr

340 345 350

Ser Val Arg Met Lys Leu Trp Thr Arg Gln Thr Tyr Lys Tyr Ile Asp

355 360 365

Asp Ala Glu Ile Glu Val Gln Arg Arg Arg Ser Ala Gly Trp Glu Ala

370 375 380

Glu Leu Arg His Arg Ala Tyr Leu His Arg Trp Gln Leu Asp Gly Lys

385 390 395 400

Leu Ser Tyr Lys Arg Gly Thr Gly Met Arg Gln Ser Met Pro Ala Pro

405 410 415

Glu Glu Asn Gly Gly Gly Thr Ile Pro Ala Thr Ser Arg Met Lys Ile

420 425 430

Ile Thr Ala Gly Leu Asp Ala Ala Ala Pro Ser Met Leu Gly Lys Gln

435 440 445

Gln Phe Phe Tyr Ala Thr Ala Ile Gln Ala Gln Trp Asn Lys Thr Pro

450 455 460

Leu Val Ala Gln Asp Lys Leu Ser Ile Gly Ser Arg Tyr Thr Val Arg

465 470 475 480

Gly Phe Asp Gly Glu Gln Ser Leu Phe Gly Glu Arg Gly Phe Tyr Trp

485 490 495

Gln Asn Thr Leu Thr Trp Tyr Phe His Pro Asn His Gln Phe Tyr Leu

500 505 510

Gly Ala Asp Tyr Gly Arg Val Ser Gly Glu Ser Ala Gln Tyr Val Ser

515 520 525

Gly Lys Gln Leu Met Gly Ala Val Val Gly Phe Arg Gly Gly His Lys

530 535 540

Val Gly Gly Met Phe Ala Tyr Asp Leu Phe Ala Gly Lys Pro Leu His

545 550 555 560

Lys Pro Lys Gly Phe Gln Thr Thr Asn Thr Val Tyr Gly Phe Asn Leu

565 570 575

Asn Tyr Ser Phe

580

<210> SEQ ID NO 21

<211> LENGTH: 411

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(408)

<400> SEQUENCE: 21

atg att gaa ttt gtc cga gcc aaa aaa cgg ctg ctt tgg gca ttt gtg 48

Met Ile Glu Phe Val Arg Ala Lys Lys Arg Leu Leu Trp Ala Phe Val

1 5 10 15

ctt ttg ctt gtg tgg acg tgc ggt tac cga tac gcc gcc gac aag gcc 96

Leu Leu Leu Val Trp Thr Cys Gly Tyr Arg Tyr Ala Ala Asp Lys Ala

20 25 30

gaa gcg aaa caa acc gcc ctg att gcc acc tat cgg cat tct tct atg 144

Glu Ala Lys Gln Thr Ala Leu Ile Ala Thr Tyr Arg His Ser Ser Met

35 40 45

gtt gcg gcg gaa caa tac gcc ttg cag ctt aaa aaa gcg cag gac gaa 192

Val Ala Ala Glu Gln Tyr Ala Leu Gln Leu Lys Lys Ala Gln Asp Glu

50 55 60

agg cag cgg tgg tac gac ttt tcc caa aaa caa gga aga aag ccc gtg 240

Arg Gln Arg Trp Tyr Asp Phe Ser Gln Lys Gln Gly Arg Lys Pro Val

65 70 75 80

aaa aaa cag tat ccg ccg caa acg aaa aaa gcc ggc tat ctg aaa acc 288

Lys Lys Gln Tyr Pro Pro Gln Thr Lys Lys Ala Gly Tyr Leu Lys Thr

85 90 95

aag gaa gaa ctg ctt gcg gaa ttg gct tgc ctt aaa gcg gaa atg gct 336

Lys Glu Glu Leu Leu Ala Glu Leu Ala Cys Leu Lys Ala Glu Met Ala

100 105 110

gcc cta aaa aag ctc gat gcc tta atc tat ggg aaa gaa gtg cgg cag 384

Ala Leu Lys Lys Leu Asp Ala Leu Ile Tyr Gly Lys Glu Val Arg Gln

115 120 125

aaa gaa cgc aac tcg tcg cag ggt taa 411

Lys Glu Arg Asn Ser Ser Gln Gly

130 135

<210> SEQ ID NO 22

<211> LENGTH: 136

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 22

Met Ile Glu Phe Val Arg Ala Lys Lys Arg Leu Leu Trp Ala Phe Val

1 5 10 15

Leu Leu Leu Val Trp Thr Cys Gly Tyr Arg Tyr Ala Ala Asp Lys Ala

20 25 30

Glu Ala Lys Gln Thr Ala Leu Ile Ala Thr Tyr Arg His Ser Ser Met

35 40 45

Val Ala Ala Glu Gln Tyr Ala Leu Gln Leu Lys Lys Ala Gln Asp Glu

50 55 60

Arg Gln Arg Trp Tyr Asp Phe Ser Gln Lys Gln Gly Arg Lys Pro Val

65 70 75 80

Lys Lys Gln Tyr Pro Pro Gln Thr Lys Lys Ala Gly Tyr Leu Lys Thr

85 90 95

Lys Glu Glu Leu Leu Ala Glu Leu Ala Cys Leu Lys Ala Glu Met Ala

100 105 110

Ala Leu Lys Lys Leu Asp Ala Leu Ile Tyr Gly Lys Glu Val Arg Gln

115 120 125

Lys Glu Arg Asn Ser Ser Gln Gly

130 135

<210> SEQ ID NO 23

<211> LENGTH: 924

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(921)

<400> SEQUENCE: 23

atg caa tac agc aca ctg gca gga caa acc gac aac tcc ctc gtt tcc 48

Met Gln Tyr Ser Thr Leu Ala Gly Gln Thr Asp Asn Ser Leu Val Ser

1 5 10 15

aat aat ttc ggg ttt ttg cgc ctg ccg ctt aat ttt atg ccg tat gaa 96

Asn Asn Phe Gly Phe Leu Arg Leu Pro Leu Asn Phe Met Pro Tyr Glu

20 25 30

agt cat gcc gat tgg gtt att acc ggc gtg cct tat gat atg gcg gtt 144

Ser His Ala Asp Trp Val Ile Thr Gly Val Pro Tyr Asp Met Ala Val

35 40 45

tca ggg cgt tcc ggc gcg cgt ttc ggt cct gaa gcc atc cgg cgc gcc 192

Ser Gly Arg Ser Gly Ala Arg Phe Gly Pro Glu Ala Ile Arg Arg Ala

50 55 60

tcc gtc aac ctc gct tgg gag cac cgc agg ttt cca tgg aca ttt gat 240

Ser Val Asn Leu Ala Trp Glu His Arg Arg Phe Pro Trp Thr Phe Asp

65 70 75 80

gtg cgc gaa cgc ctg aac att att gat tgc ggc gac ttg gtt ttt tct 288

Val Arg Glu Arg Leu Asn Ile Ile Asp Cys Gly Asp Leu Val Phe Ser

85 90 95

ttt ggc gac agc agg gat ttt gtc gaa aaa atg gaa gcg cac gcc ggc 336

Phe Gly Asp Ser Arg Asp Phe Val Glu Lys Met Glu Ala His Ala Gly

100 105 110

aaa tta ctt tct tcc ggc aaa cgc tgt ttg agt ttg ggc ggc gac cat 384

Lys Leu Leu Ser Ser Gly Lys Arg Cys Leu Ser Leu Gly Gly Asp His

115 120 125

ttc att acc ctc ccg ttg ttg cgc gcc cac gcc cgc tat ttc ggc aaa 432

Phe Ile Thr Leu Pro Leu Leu Arg Ala His Ala Arg Tyr Phe Gly Lys

130 135 140

ctc gca ctg att cat ttt gac gcg cac acc gac acc tac gac aac ggc 480

Leu Ala Leu Ile His Phe Asp Ala His Thr Asp Thr Tyr Asp Asn Gly

145 150 155 160

agc gaa tac gac cac ggt acg atg ttc tat acc gcc ccc aag gaa ggc 528

Ser Glu Tyr Asp His Gly Thr Met Phe Tyr Thr Ala Pro Lys Glu Gly

165 170 175

ctc atc gac ccg tcc cgt tcc gta caa atc ggc ata cgt acc gaa cac 576

Leu Ile Asp Pro Ser Arg Ser Val Gln Ile Gly Ile Arg Thr Glu His

180 185 190

agt aaa aaa ttg cct ttt act gtg ttg acc gcc ccc caa gtt aat gaa 624

Ser Lys Lys Leu Pro Phe Thr Val Leu Thr Ala Pro Gln Val Asn Glu

195 200 205

gac agt gtt gaa gag acc gtc cgt aaa atc aaa gaa acc gtc ggc aat 672

Asp Ser Val Glu Glu Thr Val Arg Lys Ile Lys Glu Thr Val Gly Asn

210 215 220

atg ccc gtt tac ctg act ttc gac ata gac tgc ctc gac ccg tcg ttc 720

Met Pro Val Tyr Leu Thr Phe Asp Ile Asp Cys Leu Asp Pro Ser Phe

225 230 235 240

gcc ccc ggg acc ggt acg ccc gta tgc ggc ggc ttg agc agc gac agg 768

Ala Pro Gly Thr Gly Thr Pro Val Cys Gly Gly Leu Ser Ser Asp Arg

245 250 255

gca tta aaa atc cta cgt ggg ctg acg gat ctc gac atc gtc ggt atg 816

Ala Leu Lys Ile Leu Arg Gly Leu Thr Asp Leu Asp Ile Val Gly Met

260 265 270

gat gtt gta gaa gtt gcc ccc tct tac gac caa tcc gac att acc gct 864

Asp Val Val Glu Val Ala Pro Ser Tyr Asp Gln Ser Asp Ile Thr Ala

275 280 285

ttg gcc ggc gcc aca att gcc ttg gaa atg ctt tac ctt caa ggt gcg 912

Leu Ala Gly Ala Thr Ile Ala Leu Glu Met Leu Tyr Leu Gln Gly Ala

290 295 300

aaa aag gac tga 924

Lys Lys Asp

305

<210> SEQ ID NO 24

<211> LENGTH: 307

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 24

Met Gln Tyr Ser Thr Leu Ala Gly Gln Thr Asp Asn Ser Leu Val Ser

1 5 10 15

Asn Asn Phe Gly Phe Leu Arg Leu Pro Leu Asn Phe Met Pro Tyr Glu

20 25 30

Ser His Ala Asp Trp Val Ile Thr Gly Val Pro Tyr Asp Met Ala Val

35 40 45

Ser Gly Arg Ser Gly Ala Arg Phe Gly Pro Glu Ala Ile Arg Arg Ala

50 55 60

Ser Val Asn Leu Ala Trp Glu His Arg Arg Phe Pro Trp Thr Phe Asp

65 70 75 80

Val Arg Glu Arg Leu Asn Ile Ile Asp Cys Gly Asp Leu Val Phe Ser

85 90 95

Phe Gly Asp Ser Arg Asp Phe Val Glu Lys Met Glu Ala His Ala Gly

100 105 110

Lys Leu Leu Ser Ser Gly Lys Arg Cys Leu Ser Leu Gly Gly Asp His

115 120 125

Phe Ile Thr Leu Pro Leu Leu Arg Ala His Ala Arg Tyr Phe Gly Lys

130 135 140

Leu Ala Leu Ile His Phe Asp Ala His Thr Asp Thr Tyr Asp Asn Gly

145 150 155 160

Ser Glu Tyr Asp His Gly Thr Met Phe Tyr Thr Ala Pro Lys Glu Gly

165 170 175

Leu Ile Asp Pro Ser Arg Ser Val Gln Ile Gly Ile Arg Thr Glu His

180 185 190

Ser Lys Lys Leu Pro Phe Thr Val Leu Thr Ala Pro Gln Val Asn Glu

195 200 205

Asp Ser Val Glu Glu Thr Val Arg Lys Ile Lys Glu Thr Val Gly Asn

210 215 220

Met Pro Val Tyr Leu Thr Phe Asp Ile Asp Cys Leu Asp Pro Ser Phe

225 230 235 240

Ala Pro Gly Thr Gly Thr Pro Val Cys Gly Gly Leu Ser Ser Asp Arg

245 250 255

Ala Leu Lys Ile Leu Arg Gly Leu Thr Asp Leu Asp Ile Val Gly Met

260 265 270

Asp Val Val Glu Val Ala Pro Ser Tyr Asp Gln Ser Asp Ile Thr Ala

275 280 285

Leu Ala Gly Ala Thr Ile Ala Leu Glu Met Leu Tyr Leu Gln Gly Ala

290 295 300

Lys Lys Asp

305

<210> SEQ ID NO 25

<211> LENGTH: 426

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(423)

<400> SEQUENCE: 25

atg gag cag tcg ggc aaa ttc agt tgg tct gcg gca gct ttt tgg gac 48

Met Glu Gln Ser Gly Lys Phe Ser Trp Ser Ala Ala Ala Phe Trp Asp

1 5 10 15

att ccc tac ccc gtc acc agg cgg att gcc tca agt ttg tat tcg acc 96

Ile Pro Tyr Pro Val Thr Arg Arg Ile Ala Ser Ser Leu Tyr Ser Thr

20 25 30

gaa tat ttt gtc gta tgc ttt ctg cgt ttg atg cca ctc tct ccg tgt 144

Glu Tyr Phe Val Val Cys Phe Leu Arg Leu Met Pro Leu Ser Pro Cys

35 40 45

aat ctg tat ttt gtc acc cat ctg cgt acc aat gaa tcg gaa ata gaa 192

Asn Leu Tyr Phe Val Thr His Leu Arg Thr Asn Glu Ser Glu Ile Glu

50 55 60

aga tgg tct gct gtt ccc tgc caa ata gta ttg aac gac ggc aag tcg 240

Arg Trp Ser Ala Val Pro Cys Gln Ile Val Leu Asn Asp Gly Lys Ser

65 70 75 80

gaa ttc ggc gga ttc gca ttt gaa gtg caa ctt tcc cta aca gaa aaa 288

Glu Phe Gly Gly Phe Ala Phe Glu Val Gln Leu Ser Leu Thr Glu Lys

85 90 95

ggc cag tat gcg gta gca tac gac ctt tcc tgc aag aaa gat tgc cat 336

Gly Gln Tyr Ala Val Ala Tyr Asp Leu Ser Cys Lys Lys Asp Cys His

100 105 110

gag cta cac gca act gac cca agg cga acg ata cca cat cca ata cct 384

Glu Leu His Ala Thr Asp Pro Arg Arg Thr Ile Pro His Pro Ile Pro

115 120 125

gtc ccg cca ctg cac cgt cac cga aat cgc caa aca gct taa 426

Val Pro Pro Leu His Arg His Arg Asn Arg Gln Thr Ala

130 135 140

<210> SEQ ID NO 26

<211> LENGTH: 141

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 26

Met Glu Gln Ser Gly Lys Phe Ser Trp Ser Ala Ala Ala Phe Trp Asp

1 5 10 15

Ile Pro Tyr Pro Val Thr Arg Arg Ile Ala Ser Ser Leu Tyr Ser Thr

20 25 30

Glu Tyr Phe Val Val Cys Phe Leu Arg Leu Met Pro Leu Ser Pro Cys

35 40 45

Asn Leu Tyr Phe Val Thr His Leu Arg Thr Asn Glu Ser Glu Ile Glu

50 55 60

Arg Trp Ser Ala Val Pro Cys Gln Ile Val Leu Asn Asp Gly Lys Ser

65 70 75 80

Glu Phe Gly Gly Phe Ala Phe Glu Val Gln Leu Ser Leu Thr Glu Lys

85 90 95

Gly Gln Tyr Ala Val Ala Tyr Asp Leu Ser Cys Lys Lys Asp Cys His

100 105 110

Glu Leu His Ala Thr Asp Pro Arg Arg Thr Ile Pro His Pro Ile Pro

115 120 125

Val Pro Pro Leu His Arg His Arg Asn Arg Gln Thr Ala

130 135 140

<210> SEQ ID NO 27

<211> LENGTH: 351

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(348)

<400> SEQUENCE: 27

atg caa aac ggc ggg gga aag att tac cag acg gcg gac aat gtg gaa 48

Met Gln Asn Gly Gly Gly Lys Ile Tyr Gln Thr Ala Asp Asn Val Glu

1 5 10 15

ggg att atg ctg ttg aag gta gta cct gag cgt acc gtt tcg gca gat 96

Gly Ile Met Leu Leu Lys Val Val Pro Glu Arg Thr Val Ser Ala Asp

20 25 30

gca aaa acc aga gac ccg atg tgg gac aat gcg gct tta cag acc agc 144

Ala Lys Thr Arg Asp Pro Met Trp Asp Asn Ala Ala Leu Gln Thr Ser

35 40 45

gaa ggc gta aat ttt att gct cgt ttc cta gga ttt ttt agc gat ggg 192

Glu Gly Val Asn Phe Ile Ala Arg Phe Leu Gly Phe Phe Ser Asp Gly

50 55 60

gaa tac cgc tat gtg gat gtc ctg caa ccc aac cat tcc gat att att 240

Glu Tyr Arg Tyr Val Asp Val Leu Gln Pro Asn His Ser Asp Ile Ile

65 70 75 80

cgg tat tca ggt aaa gat ttt ccg cta aat caa ata ctt aac cat ata 288

Arg Tyr Ser Gly Lys Asp Phe Pro Leu Asn Gln Ile Leu Asn His Ile

85 90 95

cac ccc gcc cgt tat gcg gta acg ttc gaa aac aat gtc gat tcc aag 336

His Pro Ala Arg Tyr Ala Val Thr Phe Glu Asn Asn Val Asp Ser Lys

100 105 110

ctg cgc agg cac tga 351

Leu Arg Arg His

115

<210> SEQ ID NO 28

<211> LENGTH: 116

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 28

Met Gln Asn Gly Gly Gly Lys Ile Tyr Gln Thr Ala Asp Asn Val Glu

1 5 10 15

Gly Ile Met Leu Leu Lys Val Val Pro Glu Arg Thr Val Ser Ala Asp

20 25 30

Ala Lys Thr Arg Asp Pro Met Trp Asp Asn Ala Ala Leu Gln Thr Ser

35 40 45

Glu Gly Val Asn Phe Ile Ala Arg Phe Leu Gly Phe Phe Ser Asp Gly

50 55 60

Glu Tyr Arg Tyr Val Asp Val Leu Gln Pro Asn His Ser Asp Ile Ile

65 70 75 80

Arg Tyr Ser Gly Lys Asp Phe Pro Leu Asn Gln Ile Leu Asn His Ile

85 90 95

His Pro Ala Arg Tyr Ala Val Thr Phe Glu Asn Asn Val Asp Ser Lys

100 105 110

Leu Arg Arg His

115

<210> SEQ ID NO 29

<211> LENGTH: 1404

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1401)

<400> SEQUENCE: 29

atg aca ttg ctc aat cta atg ata atg caa gat tac ggt att tcc gtt 48

Met Thr Leu Leu Asn Leu Met Ile Met Gln Asp Tyr Gly Ile Ser Val

1 5 10 15

tgc ctg aca ctg acg ccc tat ttg caa cat gaa cta ttt tcg gct atg 96

Cys Leu Thr Leu Thr Pro Tyr Leu Gln His Glu Leu Phe Ser Ala Met

20 25 30

aaa tcc tat ttt tcc aaa tat atc cta ccc gtt tca ctt ttt acc ttg 144

Lys Ser Tyr Phe Ser Lys Tyr Ile Leu Pro Val Ser Leu Phe Thr Leu

35 40 45

cca cta tcc ctt tcc cca tcc gtt tcg gct ttt acg ctg cct gaa gca 192

Pro Leu Ser Leu Ser Pro Ser Val Ser Ala Phe Thr Leu Pro Glu Ala

50 55 60

tgg cgg gcg gcg cag caa cat tcg gct gat ttt caa gcg tcc cat tac 240

Trp Arg Ala Ala Gln Gln His Ser Ala Asp Phe Gln Ala Ser His Tyr

65 70 75 80

cag cgt gat gca gtg cgc gca cgg caa caa caa gcc aag gcc gca ttc 288

Gln Arg Asp Ala Val Arg Ala Arg Gln Gln Gln Ala Lys Ala Ala Phe

85 90 95

ctt ccc cat gta tcc gcc aat gcc agc tac cag cgc cag ccg cca tcg 336

Leu Pro His Val Ser Ala Asn Ala Ser Tyr Gln Arg Gln Pro Pro Ser

100 105 110

att tct tcc acc cgc gaa aca cag gga tgg agc gtg cag gtg gga caa 384

Ile Ser Ser Thr Arg Glu Thr Gln Gly Trp Ser Val Gln Val Gly Gln

115 120 125

acc tta ttt gac gct gcc aaa ttt gca caa tac cgc caa agc agg ttc 432

Thr Leu Phe Asp Ala Ala Lys Phe Ala Gln Tyr Arg Gln Ser Arg Phe

130 135 140

gat acg cag gct gca gaa cag cgt ttc gat gcg gca cgc gaa gaa ttg 480

Asp Thr Gln Ala Ala Glu Gln Arg Phe Asp Ala Ala Arg Glu Glu Leu

145 150 155 160

ctg ttg aaa gtt gcc gaa agt tat ttc aac gtt tta ctc agc cga gac 528

Leu Leu Lys Val Ala Glu Ser Tyr Phe Asn Val Leu Leu Ser Arg Asp

165 170 175

acc gtt gcc gcc cat gcg gcg gaa aaa gag gct tat gcc cag cag gta 576

Thr Val Ala Ala His Ala Ala Glu Lys Glu Ala Tyr Ala Gln Gln Val

180 185 190

agg cag gcg cag gct tta ttc aat aaa ggt gct gcc acc gcg ctg gat 624

Arg Gln Ala Gln Ala Leu Phe Asn Lys Gly Ala Ala Thr Ala Leu Asp

195 200 205

att cac gaa gcc aaa gcc ggt tac gac aat gcc ctg gcc caa gaa atc 672

Ile His Glu Ala Lys Ala Gly Tyr Asp Asn Ala Leu Ala Gln Glu Ile

210 215 220

gcc gta ttg gct gag aaa caa acc tat gaa aac cag ttg aac gac tac 720

Ala Val Leu Ala Glu Lys Gln Thr Tyr Glu Asn Gln Leu Asn Asp Tyr

225 230 235 240

acc gac ctg gat agc aaa caa atc gag gcc ata gat acc gcc aac ctg 768

Thr Asp Leu Asp Ser Lys Gln Ile Glu Ala Ile Asp Thr Ala Asn Leu

245 250 255

ttg gca cgc tat ctg ccc aag ctg gaa cgt tac agt ctg gat gaa tgg 816

Leu Ala Arg Tyr Leu Pro Lys Leu Glu Arg Tyr Ser Leu Asp Glu Trp

260 265 270

cag cgc att gcc tta tcc aac aat cat gaa tac cgg atg cag cag ctt 864

Gln Arg Ile Ala Leu Ser Asn Asn His Glu Tyr Arg Met Gln Gln Leu

275 280 285

gcc ctg caa agc agc gga cag gcg ctt cgg gca gca cag aac agc cgc 912

Ala Leu Gln Ser Ser Gly Gln Ala Leu Arg Ala Ala Gln Asn Ser Arg

290 295 300

tat ccc acc gtt tct gcc cat gtc ggc tat cag aat aac ctc tac act 960

Tyr Pro Thr Val Ser Ala His Val Gly Tyr Gln Asn Asn Leu Tyr Thr

305 310 315 320

tca tct gcg cag aat aat gac tac cac tat cgg ggc aaa ggg atg agc 1008

Ser Ser Ala Gln Asn Asn Asp Tyr His Tyr Arg Gly Lys Gly Met Ser

325 330 335

gtc ggc gta cag ttg aat ttg ccg ctt tat acc ggc gga gaa ttg tcg 1056

Val Gly Val Gln Leu Asn Leu Pro Leu Tyr Thr Gly Gly Glu Leu Ser

340 345 350

ggc aaa atc cat gaa gcc gaa gcg caa tac ggg gcc gcc gaa gca cag 1104

Gly Lys Ile His Glu Ala Glu Ala Gln Tyr Gly Ala Ala Glu Ala Gln

355 360 365

ctg acc gca acc gag cgg cac atc aaa ctc gcc gta cgc cag gct tat 1152

Leu Thr Ala Thr Glu Arg His Ile Lys Leu Ala Val Arg Gln Ala Tyr

370 375 380

acc gaa agc ggt gcg gcg cgt tac caa atc atg gcg caa gaa cgg gtt 1200

Thr Glu Ser Gly Ala Ala Arg Tyr Gln Ile Met Ala Gln Glu Arg Val

385 390 395 400

ttg gaa agc agc cgt ttg aaa ctg aaa tcg acc gaa acc ggc caa caa 1248

Leu Glu Ser Ser Arg Leu Lys Leu Lys Ser Thr Glu Thr Gly Gln Gln

405 410 415

tac ggc atc cgc aac cgg ctg gaa gta ata cgg gcg cgg cag gaa gtc 1296

Tyr Gly Ile Arg Asn Arg Leu Glu Val Ile Arg Ala Arg Gln Glu Val

420 425 430

gcc caa gca gaa cag aaa ctg gct caa gca cgg tat aaa ttc atg ctg 1344

Ala Gln Ala Glu Gln Lys Leu Ala Gln Ala Arg Tyr Lys Phe Met Leu

435 440 445

gct tat ttg cgc ttg gtg aaa gag agc ggg tta ggg ttg gaa acg gta 1392

Ala Tyr Leu Arg Leu Val Lys Glu Ser Gly Leu Gly Leu Glu Thr Val

450 455 460

ttt gcg gaa taa 1404

Phe Ala Glu

465

<210> SEQ ID NO 30

<211> LENGTH: 467

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 30

Met Thr Leu Leu Asn Leu Met Ile Met Gln Asp Tyr Gly Ile Ser Val

1 5 10 15

Cys Leu Thr Leu Thr Pro Tyr Leu Gln His Glu Leu Phe Ser Ala Met

20 25 30

Lys Ser Tyr Phe Ser Lys Tyr Ile Leu Pro Val Ser Leu Phe Thr Leu

35 40 45

Pro Leu Ser Leu Ser Pro Ser Val Ser Ala Phe Thr Leu Pro Glu Ala

50 55 60

Trp Arg Ala Ala Gln Gln His Ser Ala Asp Phe Gln Ala Ser His Tyr

65 70 75 80

Gln Arg Asp Ala Val Arg Ala Arg Gln Gln Gln Ala Lys Ala Ala Phe

85 90 95

Leu Pro His Val Ser Ala Asn Ala Ser Tyr Gln Arg Gln Pro Pro Ser

100 105 110

Ile Ser Ser Thr Arg Glu Thr Gln Gly Trp Ser Val Gln Val Gly Gln

115 120 125

Thr Leu Phe Asp Ala Ala Lys Phe Ala Gln Tyr Arg Gln Ser Arg Phe

130 135 140

Asp Thr Gln Ala Ala Glu Gln Arg Phe Asp Ala Ala Arg Glu Glu Leu

145 150 155 160

Leu Leu Lys Val Ala Glu Ser Tyr Phe Asn Val Leu Leu Ser Arg Asp

165 170 175

Thr Val Ala Ala His Ala Ala Glu Lys Glu Ala Tyr Ala Gln Gln Val

180 185 190

Arg Gln Ala Gln Ala Leu Phe Asn Lys Gly Ala Ala Thr Ala Leu Asp

195 200 205

Ile His Glu Ala Lys Ala Gly Tyr Asp Asn Ala Leu Ala Gln Glu Ile

210 215 220

Ala Val Leu Ala Glu Lys Gln Thr Tyr Glu Asn Gln Leu Asn Asp Tyr

225 230 235 240

Thr Asp Leu Asp Ser Lys Gln Ile Glu Ala Ile Asp Thr Ala Asn Leu

245 250 255

Leu Ala Arg Tyr Leu Pro Lys Leu Glu Arg Tyr Ser Leu Asp Glu Trp

260 265 270

Gln Arg Ile Ala Leu Ser Asn Asn His Glu Tyr Arg Met Gln Gln Leu

275 280 285

Ala Leu Gln Ser Ser Gly Gln Ala Leu Arg Ala Ala Gln Asn Ser Arg

290 295 300

Tyr Pro Thr Val Ser Ala His Val Gly Tyr Gln Asn Asn Leu Tyr Thr

305 310 315 320

Ser Ser Ala Gln Asn Asn Asp Tyr His Tyr Arg Gly Lys Gly Met Ser

325 330 335

Val Gly Val Gln Leu Asn Leu Pro Leu Tyr Thr Gly Gly Glu Leu Ser

340 345 350

Gly Lys Ile His Glu Ala Glu Ala Gln Tyr Gly Ala Ala Glu Ala Gln

355 360 365

Leu Thr Ala Thr Glu Arg His Ile Lys Leu Ala Val Arg Gln Ala Tyr

370 375 380

Thr Glu Ser Gly Ala Ala Arg Tyr Gln Ile Met Ala Gln Glu Arg Val

385 390 395 400

Leu Glu Ser Ser Arg Leu Lys Leu Lys Ser Thr Glu Thr Gly Gln Gln

405 410 415

Tyr Gly Ile Arg Asn Arg Leu Glu Val Ile Arg Ala Arg Gln Glu Val

420 425 430

Ala Gln Ala Glu Gln Lys Leu Ala Gln Ala Arg Tyr Lys Phe Met Leu

435 440 445

Ala Tyr Leu Arg Leu Val Lys Glu Ser Gly Leu Gly Leu Glu Thr Val

450 455 460

Phe Ala Glu

465

<210> SEQ ID NO 31

<211> LENGTH: 696

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(693)

<400> SEQUENCE: 31

atg aaa caa tcc gcc cga ata aaa aat atg gat cag aca tta aaa aat 48

Met Lys Gln Ser Ala Arg Ile Lys Asn Met Asp Gln Thr Leu Lys Asn

1 5 10 15

aca ttg ggc att tgc gcg ctt tta gcc ttt tgt ttt ggc gcg gcc atc 96

Thr Leu Gly Ile Cys Ala Leu Leu Ala Phe Cys Phe Gly Ala Ala Ile

20 25 30

gca tca ggt tat cac ttg gaa tat gaa tac ggc tac cgt tat tct gcc 144

Ala Ser Gly Tyr His Leu Glu Tyr Glu Tyr Gly Tyr Arg Tyr Ser Ala

35 40 45

gtg ggt gct ttg gct tcg gtt gta ttt tta tta tta ttg gca cgc ggt 192

Val Gly Ala Leu Ala Ser Val Val Phe Leu Leu Leu Leu Ala Arg Gly

50 55 60

ttc ccg cgc gtt tct tca gtt gtt tta ctg att tac gtc ggc aca acc 240

Phe Pro Arg Val Ser Ser Val Val Leu Leu Ile Tyr Val Gly Thr Thr

65 70 75 80

gcc cta tat ttg ccg gtc ggc tgg ctg tat ggt gcg ccg tct tat cag 288

Ala Leu Tyr Leu Pro Val Gly Trp Leu Tyr Gly Ala Pro Ser Tyr Gln

85 90 95

ata gtc ggt tcg ata ttg gaa agc aat cct gcc gag gcg cgt gaa ttt 336

Ile Val Gly Ser Ile Leu Glu Ser Asn Pro Ala Glu Ala Arg Glu Phe

100 105 110

gtc ggc aat ctt ccc ggg tcg ctt tat ttt gtg cag gca tta ttt ttc 384

Val Gly Asn Leu Pro Gly Ser Leu Tyr Phe Val Gln Ala Leu Phe Phe

115 120 125

att ttt ggc ttg aca gtt tgg aga tat tgt gta tcg ggg ggg gta ttt 432

Ile Phe Gly Leu Thr Val Trp Arg Tyr Cys Val Ser Gly Gly Val Phe

130 135 140

gct gac gta aaa aac tat aaa cgc cgc agc aaa ata tgg ctg act ata 480

Ala Asp Val Lys Asn Tyr Lys Arg Arg Ser Lys Ile Trp Leu Thr Ile

145 150 155 160

tta ttg act ttg att ttg tcc tgc gcg gtg atg gat aaa atc gcc agc 528

Leu Leu Thr Leu Ile Leu Ser Cys Ala Val Met Asp Lys Ile Ala Ser

165 170 175

gat aaa gat ttg cga gaa cct gat gcc ggc ctg ttg ttg aat att ttc 576

Asp Lys Asp Leu Arg Glu Pro Asp Ala Gly Leu Leu Leu Asn Ile Phe

180 185 190

gac ctg tat tac gat ttg gct tcc gcg ccg gca cca ata tgt cgc caa 624

Asp Leu Tyr Tyr Asp Leu Ala Ser Ala Pro Ala Pro Ile Cys Arg Gln

195 200 205

gcg cgc cca cat ttt gga agc agc aaa aaa agc gtc aac atg gca tat 672

Ala Arg Pro His Phe Gly Ser Ser Lys Lys Ser Val Asn Met Ala Tyr

210 215 220

ccg tca tgt tgc gcc caa gta taa 696

Pro Ser Cys Cys Ala Gln Val

225 230

<210> SEQ ID NO 32

<211> LENGTH: 231

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 32

Met Lys Gln Ser Ala Arg Ile Lys Asn Met Asp Gln Thr Leu Lys Asn

1 5 10 15

Thr Leu Gly Ile Cys Ala Leu Leu Ala Phe Cys Phe Gly Ala Ala Ile

20 25 30

Ala Ser Gly Tyr His Leu Glu Tyr Glu Tyr Gly Tyr Arg Tyr Ser Ala

35 40 45

Val Gly Ala Leu Ala Ser Val Val Phe Leu Leu Leu Leu Ala Arg Gly

50 55 60

Phe Pro Arg Val Ser Ser Val Val Leu Leu Ile Tyr Val Gly Thr Thr

65 70 75 80

Ala Leu Tyr Leu Pro Val Gly Trp Leu Tyr Gly Ala Pro Ser Tyr Gln

85 90 95

Ile Val Gly Ser Ile Leu Glu Ser Asn Pro Ala Glu Ala Arg Glu Phe

100 105 110

Val Gly Asn Leu Pro Gly Ser Leu Tyr Phe Val Gln Ala Leu Phe Phe

115 120 125

Ile Phe Gly Leu Thr Val Trp Arg Tyr Cys Val Ser Gly Gly Val Phe

130 135 140

Ala Asp Val Lys Asn Tyr Lys Arg Arg Ser Lys Ile Trp Leu Thr Ile

145 150 155 160

Leu Leu Thr Leu Ile Leu Ser Cys Ala Val Met Asp Lys Ile Ala Ser

165 170 175

Asp Lys Asp Leu Arg Glu Pro Asp Ala Gly Leu Leu Leu Asn Ile Phe

180 185 190

Asp Leu Tyr Tyr Asp Leu Ala Ser Ala Pro Ala Pro Ile Cys Arg Gln

195 200 205

Ala Arg Pro His Phe Gly Ser Ser Lys Lys Ser Val Asn Met Ala Tyr

210 215 220

Pro Ser Cys Cys Ala Gln Val

225 230

<210> SEQ ID NO 33

<211> LENGTH: 909

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(906)

<400> SEQUENCE: 33

atg aat gtt tac ggt ttc cca ttg ccc gat acg cct ttt ttg agt cgg 48

Met Asn Val Tyr Gly Phe Pro Leu Pro Asp Thr Pro Phe Leu Ser Arg

1 5 10 15

acc aaa ggg ctg ttg ata aac ggt tac cat ttc acc gcc cac gcg acg 96

Thr Lys Gly Leu Leu Ile Asn Gly Tyr His Phe Thr Ala His Ala Thr

20 25 30

aat ctt tcg ctg ccg cag act ttg ggg ctg ccg gga gag ccg aac aat 144

Asn Leu Ser Leu Pro Gln Thr Leu Gly Leu Pro Gly Glu Pro Asn Asn

35 40 45

aac att gtc agc ttg gcg aag cag gcg ggt ttt cgg acg gcg tgg ctg 192

Asn Ile Val Ser Leu Ala Lys Gln Ala Gly Phe Arg Thr Ala Trp Leu

50 55 60

tct aat caa gga atg ttg ggg cat ttt gcc aac gaa att tcc acc tat 240

Ser Asn Gln Gly Met Leu Gly His Phe Ala Asn Glu Ile Ser Thr Tyr

65 70 75 80

gcc cta cgc agc gat tat ccg tgg ttt acc caa agg ggt gat tat ggc 288

Ala Leu Arg Ser Asp Tyr Pro Trp Phe Thr Gln Arg Gly Asp Tyr Gly

85 90 95

aaa agc gcg ggg ttg agc gac cgc ctt ttg ttg ccg gcg ttc aaa cgg 336

Lys Ser Ala Gly Leu Ser Asp Arg Leu Leu Leu Pro Ala Phe Lys Arg

100 105 110

gtt ttg ata gga aat gca ggc acg aag cct cgg ctg att gtg atg cac 384

Val Leu Ile Gly Asn Ala Gly Thr Lys Pro Arg Leu Ile Val Met His

115 120 125

ctg atg ggt tcg cac agt gat ttt tgc aca cgt ttg gat aag gat gcg 432

Leu Met Gly Ser His Ser Asp Phe Cys Thr Arg Leu Asp Lys Asp Ala

130 135 140

cgg cgg ttt cag tat caa act gaa aaa ata tcc tgc tat gtt tcc acc 480

Arg Arg Phe Gln Tyr Gln Thr Glu Lys Ile Ser Cys Tyr Val Ser Thr

145 150 155 160

atc gcg caa acc gat aaa ttt tta gaa gat aca gtt aag ata ttg aat 528

Ile Ala Gln Thr Asp Lys Phe Leu Glu Asp Thr Val Lys Ile Leu Asn

165 170 175

gaa aat aaa gaa agc tgg tct ttg gtt tac ttt tcc gac cac ggt ttg 576

Glu Asn Lys Glu Ser Trp Ser Leu Val Tyr Phe Ser Asp His Gly Leu

180 185 190

atg cat gtc ggt aaa ggc ggc gag cga acg ttg aca cat ggt gcg tgg 624

Met His Val Gly Lys Gly Gly Glu Arg Thr Leu Thr His Gly Ala Trp

195 200 205

aag cgt caa agc tac ggc gtg ccg ctg gtt aaa att tcg tcc gat gac 672

Lys Arg Gln Ser Tyr Gly Val Pro Leu Val Lys Ile Ser Ser Asp Asp

210 215 220

acg cgg cgc gaa atg att aaa gtg agg cgc agc gcg ttt aat ttt tta 720

Thr Arg Arg Glu Met Ile Lys Val Arg Arg Ser Ala Phe Asn Phe Leu

225 230 235 240

cgc gga ttc ggc agt tgg acg ggt atc gaa acc gac gag ttg ccc gat 768

Arg Gly Phe Gly Ser Trp Thr Gly Ile Glu Thr Asp Glu Leu Pro Asp

245 250 255

gac ggc tat gat ttt tgg ggg aat gtt ccc gat gtg cag ggc gaa ggc 816

Asp Gly Tyr Asp Phe Trp Gly Asn Val Pro Asp Val Gln Gly Glu Gly

260 265 270

aat aac ctt gcc ttt atc gac gga ctg ccc gac gac ccc gcg ccg tgg 864

Asn Asn Leu Ala Phe Ile Asp Gly Leu Pro Asp Asp Pro Ala Pro Trp

275 280 285

tat gcg gga aaa ggc aaa tcg act aaa aat acg tct aaa aaa tga 909

Tyr Ala Gly Lys Gly Lys Ser Thr Lys Asn Thr Ser Lys Lys

290 295 300

<210> SEQ ID NO 34

<211> LENGTH: 302

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 34

Met Asn Val Tyr Gly Phe Pro Leu Pro Asp Thr Pro Phe Leu Ser Arg

1 5 10 15

Thr Lys Gly Leu Leu Ile Asn Gly Tyr His Phe Thr Ala His Ala Thr

20 25 30

Asn Leu Ser Leu Pro Gln Thr Leu Gly Leu Pro Gly Glu Pro Asn Asn

35 40 45

Asn Ile Val Ser Leu Ala Lys Gln Ala Gly Phe Arg Thr Ala Trp Leu

50 55 60

Ser Asn Gln Gly Met Leu Gly His Phe Ala Asn Glu Ile Ser Thr Tyr

65 70 75 80

Ala Leu Arg Ser Asp Tyr Pro Trp Phe Thr Gln Arg Gly Asp Tyr Gly

85 90 95

Lys Ser Ala Gly Leu Ser Asp Arg Leu Leu Leu Pro Ala Phe Lys Arg

100 105 110

Val Leu Ile Gly Asn Ala Gly Thr Lys Pro Arg Leu Ile Val Met His

115 120 125

Leu Met Gly Ser His Ser Asp Phe Cys Thr Arg Leu Asp Lys Asp Ala

130 135 140

Arg Arg Phe Gln Tyr Gln Thr Glu Lys Ile Ser Cys Tyr Val Ser Thr

145 150 155 160

Ile Ala Gln Thr Asp Lys Phe Leu Glu Asp Thr Val Lys Ile Leu Asn

165 170 175

Glu Asn Lys Glu Ser Trp Ser Leu Val Tyr Phe Ser Asp His Gly Leu

180 185 190

Met His Val Gly Lys Gly Gly Glu Arg Thr Leu Thr His Gly Ala Trp

195 200 205

Lys Arg Gln Ser Tyr Gly Val Pro Leu Val Lys Ile Ser Ser Asp Asp

210 215 220

Thr Arg Arg Glu Met Ile Lys Val Arg Arg Ser Ala Phe Asn Phe Leu

225 230 235 240

Arg Gly Phe Gly Ser Trp Thr Gly Ile Glu Thr Asp Glu Leu Pro Asp

245 250 255

Asp Gly Tyr Asp Phe Trp Gly Asn Val Pro Asp Val Gln Gly Glu Gly

260 265 270

Asn Asn Leu Ala Phe Ile Asp Gly Leu Pro Asp Asp Pro Ala Pro Trp

275 280 285

Tyr Ala Gly Lys Gly Lys Ser Thr Lys Asn Thr Ser Lys Lys

290 295 300

<210> SEQ ID NO 35

<211> LENGTH: 864

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(861)

<400> SEQUENCE: 35

atg atg agt caa cac tct gcc gga gca cgt ttc cgc caa gcc gtg aaa 48

Met Met Ser Gln His Ser Ala Gly Ala Arg Phe Arg Gln Ala Val Lys

1 5 10 15

gaa tcg aat ccg ctt gcc gtc gcc ggt tgc gtc aat gct tat ttt gca 96

Glu Ser Asn Pro Leu Ala Val Ala Gly Cys Val Asn Ala Tyr Phe Ala

20 25 30

cga ttg gcc acc caa agc ggt ttc aaa gcc atc tat ctg tcc ggc ggc 144

Arg Leu Ala Thr Gln Ser Gly Phe Lys Ala Ile Tyr Leu Ser Gly Gly

35 40 45

ggc gtg gca gcc tgt tct tgc ggt atc cct gat ttg ggc att acc aca 192

Gly Val Ala Ala Cys Ser Cys Gly Ile Pro Asp Leu Gly Ile Thr Thr

50 55 60

atg gaa gat gtg ctg atc gac gca cga cgc att acg gac aac gtg gat 240

Met Glu Asp Val Leu Ile Asp Ala Arg Arg Ile Thr Asp Asn Val Asp

65 70 75 80

acg cct ctg ctg gtg gac atc gat gtg ggt tgg ggc ggt gca ttc aat 288

Thr Pro Leu Leu Val Asp Ile Asp Val Gly Trp Gly Gly Ala Phe Asn

85 90 95

att gcc cgt acc att cgc aac ttt gaa cgc gcc ggt gtt gca gcg gtt 336

Ile Ala Arg Thr Ile Arg Asn Phe Glu Arg Ala Gly Val Ala Ala Val

100 105 110

cac atc gaa gat cag gta gcg caa aaa cgc tgc ggc cac cgt ccg aac 384

His Ile Glu Asp Gln Val Ala Gln Lys Arg Cys Gly His Arg Pro Asn

115 120 125

aaa gcc att gta tct aaa gat gaa atg gtc gac cgt atc aaa gct gcc 432

Lys Ala Ile Val Ser Lys Asp Glu Met Val Asp Arg Ile Lys Ala Ala

130 135 140

gta gat gcg cgc gtt gat gag aac ttc gtg att atg gcg cgt acc gat 480

Val Asp Ala Arg Val Asp Glu Asn Phe Val Ile Met Ala Arg Thr Asp

145 150 155 160

gcg ctg gcg gta gaa ggt ttg gat gcc gct atc gaa cgc gcc caa gct 528

Ala Leu Ala Val Glu Gly Leu Asp Ala Ala Ile Glu Arg Ala Gln Ala

165 170 175

tgt gtc gaa gcc ggt gcg gac atg att ttc cct gaa gcc atg acc gat 576

Cys Val Glu Ala Gly Ala Asp Met Ile Phe Pro Glu Ala Met Thr Asp

180 185 190

ttg aac atg tac cgc caa ttt gca gat gcg gtg aaa gtg ccc gtg ttg 624

Leu Asn Met Tyr Arg Gln Phe Ala Asp Ala Val Lys Val Pro Val Leu

195 200 205

gcg aac att acc gag ttt ggt tcc act ccg ctt tat acc caa agc gag 672

Ala Asn Ile Thr Glu Phe Gly Ser Thr Pro Leu Tyr Thr Gln Ser Glu

210 215 220

ctg gct gaa aac ggc gtg tcg ctg gtg ctg tat ccg ctg tca tcg ttc 720

Leu Ala Glu Asn Gly Val Ser Leu Val Leu Tyr Pro Leu Ser Ser Phe

225 230 235 240

cgt gca gca agc aaa gcc gct ctg aat gtt tac gaa gcg att atg cgc 768

Arg Ala Ala Ser Lys Ala Ala Leu Asn Val Tyr Glu Ala Ile Met Arg

245 250 255

gat ggc act tca ggc ggc ggt ggt gga cag tat gca aac ccg tgc cga 816

Asp Gly Thr Ser Gly Gly Gly Gly Gly Gln Tyr Ala Asn Pro Cys Arg

260 265 270

gct gta cga gca tct gaa cta tca tgc ctt cga gca aaa act gga taa 864

Ala Val Arg Ala Ser Glu Leu Ser Cys Leu Arg Ala Lys Thr Gly

275 280 285

<210> SEQ ID NO 36

<211> LENGTH: 287

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 36

Met Met Ser Gln His Ser Ala Gly Ala Arg Phe Arg Gln Ala Val Lys

1 5 10 15

Glu Ser Asn Pro Leu Ala Val Ala Gly Cys Val Asn Ala Tyr Phe Ala

20 25 30

Arg Leu Ala Thr Gln Ser Gly Phe Lys Ala Ile Tyr Leu Ser Gly Gly

35 40 45

Gly Val Ala Ala Cys Ser Cys Gly Ile Pro Asp Leu Gly Ile Thr Thr

50 55 60

Met Glu Asp Val Leu Ile Asp Ala Arg Arg Ile Thr Asp Asn Val Asp

65 70 75 80

Thr Pro Leu Leu Val Asp Ile Asp Val Gly Trp Gly Gly Ala Phe Asn

85 90 95

Ile Ala Arg Thr Ile Arg Asn Phe Glu Arg Ala Gly Val Ala Ala Val

100 105 110

His Ile Glu Asp Gln Val Ala Gln Lys Arg Cys Gly His Arg Pro Asn

115 120 125

Lys Ala Ile Val Ser Lys Asp Glu Met Val Asp Arg Ile Lys Ala Ala

130 135 140

Val Asp Ala Arg Val Asp Glu Asn Phe Val Ile Met Ala Arg Thr Asp

145 150 155 160

Ala Leu Ala Val Glu Gly Leu Asp Ala Ala Ile Glu Arg Ala Gln Ala

165 170 175

Cys Val Glu Ala Gly Ala Asp Met Ile Phe Pro Glu Ala Met Thr Asp

180 185 190

Leu Asn Met Tyr Arg Gln Phe Ala Asp Ala Val Lys Val Pro Val Leu

195 200 205

Ala Asn Ile Thr Glu Phe Gly Ser Thr Pro Leu Tyr Thr Gln Ser Glu

210 215 220

Leu Ala Glu Asn Gly Val Ser Leu Val Leu Tyr Pro Leu Ser Ser Phe

225 230 235 240

Arg Ala Ala Ser Lys Ala Ala Leu Asn Val Tyr Glu Ala Ile Met Arg

245 250 255

Asp Gly Thr Ser Gly Gly Gly Gly Gly Gln Tyr Ala Asn Pro Cys Arg

260 265 270

Ala Val Arg Ala Ser Glu Leu Ser Cys Leu Arg Ala Lys Thr Gly

275 280 285

<210> SEQ ID NO 37

<211> LENGTH: 921

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(918)

<400> SEQUENCE: 37

atg cct tcg agc aaa aac tgg ata aat tgt ttc aaa aat gat tta ccg 48

Met Pro Ser Ser Lys Asn Trp Ile Asn Cys Phe Lys Asn Asp Leu Pro

1 5 10 15

ctt tca gac tgc ctt tca aca aat ccg cat cgg tcg tct gaa aac ccg 96

Leu Ser Asp Cys Leu Ser Thr Asn Pro His Arg Ser Ser Glu Asn Pro

20 25 30

aaa ccc ata aaa aca caa agg aga aat acc atg act gaa act act caa 144

Lys Pro Ile Lys Thr Gln Arg Arg Asn Thr Met Thr Glu Thr Thr Gln

35 40 45

acc ccg acc ctc aaa cct aaa aaa tcc gtt gcg ctt tct ggc gtt gcg 192

Thr Pro Thr Leu Lys Pro Lys Lys Ser Val Ala Leu Ser Gly Val Ala

50 55 60

gcc ggt aat acc gct ttg tgt acc gtt ggc cgt acc ggc aac gat ttg 240

Ala Gly Asn Thr Ala Leu Cys Thr Val Gly Arg Thr Gly Asn Asp Leu

65 70 75 80

agc tat cgc ggt tac gac att ctg gat ttg gca caa aaa tgt gag ttt 288

Ser Tyr Arg Gly Tyr Asp Ile Leu Asp Leu Ala Gln Lys Cys Glu Phe

85 90 95

gaa gaa gtt gcc cac ctg ctg att cac ggc cat tta ccc aac aaa ttc 336

Glu Glu Val Ala His Leu Leu Ile His Gly His Leu Pro Asn Lys Phe

100 105 110

gag ctg gcc gct tat aaa gcc aag ctc aaa tcc atg cgc ggc ctg cct 384

Glu Leu Ala Ala Tyr Lys Ala Lys Leu Lys Ser Met Arg Gly Leu Pro

115 120 125

atc cgt gtg att aaa gtt ttg gaa agc ctg cct gca cat acc cat ccg 432

Ile Arg Val Ile Lys Val Leu Glu Ser Leu Pro Ala His Thr His Pro

130 135 140

atg gac gtg atg cgt acc ggc gta tcc atg ctg ggc tgt gtt cat cct 480

Met Asp Val Met Arg Thr Gly Val Ser Met Leu Gly Cys Val His Pro

145 150 155 160

gaa cgt gaa ggc cat ccg gaa agc gaa gcg cgc gac att gcc gac aaa 528

Glu Arg Glu Gly His Pro Glu Ser Glu Ala Arg Asp Ile Ala Asp Lys

165 170 175

ctg atc gcc agc ctc ggc agt atc ctc ttg tac tgg tat caa tat tcg 576

Leu Ile Ala Ser Leu Gly Ser Ile Leu Leu Tyr Trp Tyr Gln Tyr Ser

180 185 190

cac aac ggc aaa cgc att gaa gtt gaa agc gaa gaa gag acc atc ggc 624

His Asn Gly Lys Arg Ile Glu Val Glu Ser Glu Glu Glu Thr Ile Gly

195 200 205

ggt cat ttc ctg cac ctg ttg cac ggc aaa cgc cca agc gaa tca cac 672

Gly His Phe Leu His Leu Leu His Gly Lys Arg Pro Ser Glu Ser His

210 215 220

atc aaa gcc atg cac gtt tca ctg att ctg tat gcc gaa cac gag ttc 720

Ile Lys Ala Met His Val Ser Leu Ile Leu Tyr Ala Glu His Glu Phe

225 230 235 240

aac gct tct acc ttt acc gcc cgc gtg atc gcc ggt aca ggc tct gat 768

Asn Ala Ser Thr Phe Thr Ala Arg Val Ile Ala Gly Thr Gly Ser Asp

245 250 255

atg tac tcc agc att acc gga gca atc ggc gcg ttg aaa ggt ccg aaa 816

Met Tyr Ser Ser Ile Thr Gly Ala Ile Gly Ala Leu Lys Gly Pro Lys

260 265 270

cac ggc ggc gcg aac gaa ggg ctt acg ata ttc aaa aac gct acc gca 864

His Gly Gly Ala Asn Glu Gly Leu Thr Ile Phe Lys Asn Ala Thr Ala

275 280 285

atg ccg acg aag ccg aag ccg aca tcc gcg aac gca tcg gcc gca aag 912

Met Pro Thr Lys Pro Lys Pro Thr Ser Ala Asn Ala Ser Ala Ala Lys

290 295 300

aaa tcg tga 921

Lys Ser

305

<210> SEQ ID NO 38

<211> LENGTH: 306

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 38

Met Pro Ser Ser Lys Asn Trp Ile Asn Cys Phe Lys Asn Asp Leu Pro

1 5 10 15

Leu Ser Asp Cys Leu Ser Thr Asn Pro His Arg Ser Ser Glu Asn Pro

20 25 30

Lys Pro Ile Lys Thr Gln Arg Arg Asn Thr Met Thr Glu Thr Thr Gln

35 40 45

Thr Pro Thr Leu Lys Pro Lys Lys Ser Val Ala Leu Ser Gly Val Ala

50 55 60

Ala Gly Asn Thr Ala Leu Cys Thr Val Gly Arg Thr Gly Asn Asp Leu

65 70 75 80

Ser Tyr Arg Gly Tyr Asp Ile Leu Asp Leu Ala Gln Lys Cys Glu Phe

85 90 95

Glu Glu Val Ala His Leu Leu Ile His Gly His Leu Pro Asn Lys Phe

100 105 110

Glu Leu Ala Ala Tyr Lys Ala Lys Leu Lys Ser Met Arg Gly Leu Pro

115 120 125

Ile Arg Val Ile Lys Val Leu Glu Ser Leu Pro Ala His Thr His Pro

130 135 140

Met Asp Val Met Arg Thr Gly Val Ser Met Leu Gly Cys Val His Pro

145 150 155 160

Glu Arg Glu Gly His Pro Glu Ser Glu Ala Arg Asp Ile Ala Asp Lys

165 170 175

Leu Ile Ala Ser Leu Gly Ser Ile Leu Leu Tyr Trp Tyr Gln Tyr Ser

180 185 190

His Asn Gly Lys Arg Ile Glu Val Glu Ser Glu Glu Glu Thr Ile Gly

195 200 205

Gly His Phe Leu His Leu Leu His Gly Lys Arg Pro Ser Glu Ser His

210 215 220

Ile Lys Ala Met His Val Ser Leu Ile Leu Tyr Ala Glu His Glu Phe

225 230 235 240

Asn Ala Ser Thr Phe Thr Ala Arg Val Ile Ala Gly Thr Gly Ser Asp

245 250 255

Met Tyr Ser Ser Ile Thr Gly Ala Ile Gly Ala Leu Lys Gly Pro Lys

260 265 270

His Gly Gly Ala Asn Glu Gly Leu Thr Ile Phe Lys Asn Ala Thr Ala

275 280 285

Met Pro Thr Lys Pro Lys Pro Thr Ser Ala Asn Ala Ser Ala Ala Lys

290 295 300

Lys Ser

305

<210> SEQ ID NO 39

<211> LENGTH: 945

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(942)

<400> SEQUENCE: 39

atg cac cta tgt gga aag tat tat gga gta aat atg aag ctg cgt gat 48

Met His Leu Cys Gly Lys Tyr Tyr Gly Val Asn Met Lys Leu Arg Asp

1 5 10 15

tta ctg atg gga ata ttc ttg gca gtt tct gcg gcc ctt ctg aat gca 96

Leu Leu Met Gly Ile Phe Leu Ala Val Ser Ala Ala Leu Leu Asn Ala

20 25 30

acc atc ggc ata ttc agc aag ata ttg atg gag cag ggc ttg tct gtt 144

Thr Ile Gly Ile Phe Ser Lys Ile Leu Met Glu Gln Gly Leu Ser Val

35 40 45

cag cat att gca ttt ttg aaa act ttg aca ggt ttc gtg ttt atc agc 192

Gln His Ile Ala Phe Leu Lys Thr Leu Thr Gly Phe Val Phe Ile Ser

50 55 60

att ttg ctt tgc cgt acc ggt ttt acc aga cag att gcg gat att tca 240

Ile Leu Leu Cys Arg Thr Gly Phe Thr Arg Gln Ile Ala Asp Ile Ser

65 70 75 80

aga aag aaa gag gca att ttg ccg ttg ctg tta aaa gta gca att tgt 288

Arg Lys Lys Glu Ala Ile Leu Pro Leu Leu Leu Lys Val Ala Ile Cys

85 90 95

gct ttt ttc gga att tat acg ttg ttt ttc ttt gaa acc aca gct tat 336

Ala Phe Phe Gly Ile Tyr Thr Leu Phe Phe Phe Glu Thr Thr Ala Tyr

100 105 110

caa tat ggc aat gct gcg aat gta gta gtt gta tta atg gca tcg gct 384

Gln Tyr Gly Asn Ala Ala Asn Val Val Val Val Leu Met Ala Ser Ala

115 120 125

gcc gta tct gcc ttg ata ttg gac agc ata ctg tta gat gaa cgt att 432

Ala Val Ser Ala Leu Ile Leu Asp Ser Ile Leu Leu Asp Glu Arg Ile

130 135 140

tgc att tct tca gtc gtc ggt gtg ggt ttg gca gta ttg ggg atc gca 480

Cys Ile Ser Ser Val Val Gly Val Gly Leu Ala Val Leu Gly Ile Ala

145 150 155 160

atg att tct tgg act gga gaa gga agt tta ggg ttg att ctg aat gcc 528

Met Ile Ser Trp Thr Gly Glu Gly Ser Leu Gly Leu Ile Leu Asn Ala

165 170 175

gca ctg gcg ggc tcg ggc tac ggt tgt ttt tcc gtt ttg att aag aaa 576

Ala Leu Ala Gly Ser Gly Tyr Gly Cys Phe Ser Val Leu Ile Lys Lys

180 185 190

ttc ggc cta aac ggc ggt att tat ttg aca cgg ata ttg atg ttt ttt 624

Phe Gly Leu Asn Gly Gly Ile Tyr Leu Thr Arg Ile Leu Met Phe Phe

195 200 205

gga agt att ttt ttg ttt atc cct tca ttg gaa ggt att gag gat ata 672

Gly Ser Ile Phe Leu Phe Ile Pro Ser Leu Glu Gly Ile Glu Asp Ile

210 215 220

cat tgg caa tgg tct ttt att ccg cca ctc ttg gca ttg tct tta ttg 720

His Trp Gln Trp Ser Phe Ile Pro Pro Leu Leu Ala Leu Ser Leu Leu

225 230 235 240

ccg acg att tta gga ttt tat tgt aca act aaa gca ttg gat tat ttg 768

Pro Thr Ile Leu Gly Phe Tyr Cys Thr Thr Lys Ala Leu Asp Tyr Leu

245 250 255

agt gct gcg aag gta cag gta act gaa ttg gcc gag cca ttg ttt gct 816

Ser Ala Ala Lys Val Gln Val Thr Glu Leu Ala Glu Pro Leu Phe Ala

260 265 270

gcc gta ctg gct tgg ttg ttt ttg aat gaa ata ccg gaa gga cgc ttc 864

Ala Val Leu Ala Trp Leu Phe Leu Asn Glu Ile Pro Glu Gly Arg Phe

275 280 285

ttt gtc ggc gcc att ctg att att gcc ggt att gtg tct atc aat ggg 912

Phe Val Gly Ala Ile Leu Ile Ile Ala Gly Ile Val Ser Ile Asn Gly

290 295 300

ctg tat cga cca ttg ttg aag cga att gaa taa 945

Leu Tyr Arg Pro Leu Leu Lys Arg Ile Glu

305 310

<210> SEQ ID NO 40

<211> LENGTH: 314

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 40

Met His Leu Cys Gly Lys Tyr Tyr Gly Val Asn Met Lys Leu Arg Asp

1 5 10 15

Leu Leu Met Gly Ile Phe Leu Ala Val Ser Ala Ala Leu Leu Asn Ala

20 25 30

Thr Ile Gly Ile Phe Ser Lys Ile Leu Met Glu Gln Gly Leu Ser Val

35 40 45

Gln His Ile Ala Phe Leu Lys Thr Leu Thr Gly Phe Val Phe Ile Ser

50 55 60

Ile Leu Leu Cys Arg Thr Gly Phe Thr Arg Gln Ile Ala Asp Ile Ser

65 70 75 80

Arg Lys Lys Glu Ala Ile Leu Pro Leu Leu Leu Lys Val Ala Ile Cys

85 90 95

Ala Phe Phe Gly Ile Tyr Thr Leu Phe Phe Phe Glu Thr Thr Ala Tyr

100 105 110

Gln Tyr Gly Asn Ala Ala Asn Val Val Val Val Leu Met Ala Ser Ala

115 120 125

Ala Val Ser Ala Leu Ile Leu Asp Ser Ile Leu Leu Asp Glu Arg Ile

130 135 140

Cys Ile Ser Ser Val Val Gly Val Gly Leu Ala Val Leu Gly Ile Ala

145 150 155 160

Met Ile Ser Trp Thr Gly Glu Gly Ser Leu Gly Leu Ile Leu Asn Ala

165 170 175

Ala Leu Ala Gly Ser Gly Tyr Gly Cys Phe Ser Val Leu Ile Lys Lys

180 185 190

Phe Gly Leu Asn Gly Gly Ile Tyr Leu Thr Arg Ile Leu Met Phe Phe

195 200 205

Gly Ser Ile Phe Leu Phe Ile Pro Ser Leu Glu Gly Ile Glu Asp Ile

210 215 220

His Trp Gln Trp Ser Phe Ile Pro Pro Leu Leu Ala Leu Ser Leu Leu

225 230 235 240

Pro Thr Ile Leu Gly Phe Tyr Cys Thr Thr Lys Ala Leu Asp Tyr Leu

245 250 255

Ser Ala Ala Lys Val Gln Val Thr Glu Leu Ala Glu Pro Leu Phe Ala

260 265 270

Ala Val Leu Ala Trp Leu Phe Leu Asn Glu Ile Pro Glu Gly Arg Phe

275 280 285

Phe Val Gly Ala Ile Leu Ile Ile Ala Gly Ile Val Ser Ile Asn Gly

290 295 300

Leu Tyr Arg Pro Leu Leu Lys Arg Ile Glu

305 310

<210> SEQ ID NO 41

<211> LENGTH: 2610

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(2607)

<400> SEQUENCE: 41

atg gct gcc aac caa cgt tac cgc aaa ccg ctg ccc ggt acg gat ttg 48

Met Ala Ala Asn Gln Arg Tyr Arg Lys Pro Leu Pro Gly Thr Asp Leu

1 5 10 15

gaa tac tac gac gcg cgt gcg gcg tgt gag gac atc aag ccc ggc tct 96

Glu Tyr Tyr Asp Ala Arg Ala Ala Cys Glu Asp Ile Lys Pro Gly Ser

20 25 30

tac gac aag ctg cct tac acg agc cgc att ttg gcg gag aat ttg gtc 144

Tyr Asp Lys Leu Pro Tyr Thr Ser Arg Ile Leu Ala Glu Asn Leu Val

35 40 45

aac cgc gcg gac aaa gtc gat ttg ccg acg ctg caa agc tgg ctg ggt 192

Asn Arg Ala Asp Lys Val Asp Leu Pro Thr Leu Gln Ser Trp Leu Gly

50 55 60

cag ctg att gag gga aaa cag gaa atc gac ttt cct tgg tat ccg gcg 240

Gln Leu Ile Glu Gly Lys Gln Glu Ile Asp Phe Pro Trp Tyr Pro Ala

65 70 75 80

cgg gtg gtg tgc cac gat att ctg ggg cag acc gcg ttg gtg gat ttg 288

Arg Val Val Cys His Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu

85 90 95

gca ggt ctg cgc gat gcg att gcc gaa aaa ggc ggc gat cct gcc aaa 336

Ala Gly Leu Arg Asp Ala Ile Ala Glu Lys Gly Gly Asp Pro Ala Lys

100 105 110

gtg aat ccg gtg gtt gca aaa ccc agc ttc atc gtc gac cac tct ctg 384

Val Asn Pro Val Val Ala Lys Pro Ser Phe Ile Val Asp His Ser Leu

115 120 125

gcc gtt gaa tgc ggc ggc tac gac ccc gat gcc ttc cgc aaa aac cgc 432

Ala Val Glu Cys Gly Gly Tyr Asp Pro Asp Ala Phe Arg Lys Asn Arg

130 135 140

caa atc gaa gac aga cgt aac gaa gac cgt ttc cac ttc atc aac tgg 480

Gln Ile Glu Asp Arg Arg Asn Glu Asp Arg Phe His Phe Ile Asn Trp

145 150 155 160

aca aaa acc gca ttt gaa aat gtg gac gtg att ccg gcg ggc aac ggc 528

Thr Lys Thr Ala Phe Glu Asn Val Asp Val Ile Pro Ala Gly Asn Gly

165 170 175

atc atg cac caa atc aat cta gaa aaa atg tcg ccc gtc gtc caa gtc 576

Ile Met His Gln Ile Asn Leu Glu Lys Met Ser Pro Val Val Gln Val

180 185 190

aaa aac ggc gtg gcg ttc ccc gat acc tgc gtc ggc acg gat tcg cac 624

Lys Asn Gly Val Ala Phe Pro Asp Thr Cys Val Gly Thr Asp Ser His

195 200 205

acg ccg cac gtc gat gcg ctg ggc gtg att tcc gtg ggc gtg ggc gga 672

Thr Pro His Val Asp Ala Leu Gly Val Ile Ser Val Gly Val Gly Gly

210 215 220

ttg gaa gcg gaa acc gtg atg ctg ggt cgc gcg tcc atg atg cgc ctg 720

Leu Glu Ala Glu Thr Val Met Leu Gly Arg Ala Ser Met Met Arg Leu

225 230 235 240

ccc gat att gtc ggc gtt gag ctg aac ggc aaa cgg cag gcg ggc att 768

Pro Asp Ile Val Gly Val Glu Leu Asn Gly Lys Arg Gln Ala Gly Ile

245 250 255

acg gcg acg gat att gtg ttg gca ctg acc gag ttt ctg cgc aaa gaa 816

Thr Ala Thr Asp Ile Val Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu

260 265 270

cgc gtg gtc ggg gcg ttt gtc gaa ttc ttc ggc gag ggc gcg aga agc 864

Arg Val Val Gly Ala Phe Val Glu Phe Phe Gly Glu Gly Ala Arg Ser

275 280 285

ctg tct atc ggc gac cgc gcg acc att tcc aac atg acg ccg gag ttc 912

Leu Ser Ile Gly Asp Arg Ala Thr Ile Ser Asn Met Thr Pro Glu Phe

290 295 300

ggc gcg act gcc gcg atg ttc gct att gat gag caa acc att gat tat 960

Gly Ala Thr Ala Ala Met Phe Ala Ile Asp Glu Gln Thr Ile Asp Tyr

305 310 315 320

ttg aaa ctg acc gga cgc gac gac gcg cag gtg aaa ttg gtg gaa acc 1008

Leu Lys Leu Thr Gly Arg Asp Asp Ala Gln Val Lys Leu Val Glu Thr

325 330 335

tac gcc aaa acc gca ggc tta tgg gca gat gcc ttg aaa acc gcc gtt 1056

Tyr Ala Lys Thr Ala Gly Leu Trp Ala Asp Ala Leu Lys Thr Ala Val

340 345 350

tat ccg cgc gtt ttg aaa ttt gat ttg agc agc gta acg cgc aat atg 1104

Tyr Pro Arg Val Leu Lys Phe Asp Leu Ser Ser Val Thr Arg Asn Met

355 360 365

gca ggc ccg agc aac ccg cac gcg cgt ttt gcg acc gcc gat ttg gcc 1152

Ala Gly Pro Ser Asn Pro His Ala Arg Phe Ala Thr Ala Asp Leu Ala

370 375 380

agc aaa ggc ttg gct aaa cct tac gaa gag cct tca gac ggc caa atg 1200

Ser Lys Gly Leu Ala Lys Pro Tyr Glu Glu Pro Ser Asp Gly Gln Met

385 390 395 400

ccc gac ggc gcg gtc atc atc gcc gcg att acc agt tgc acc aac act 1248

Pro Asp Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr

405 410 415

tcc aac ccg cgc aac gtt gtt gcc gcc gcg ctc ttg gcg cgc aac gcc 1296

Ser Asn Pro Arg Asn Val Val Ala Ala Ala Leu Leu Ala Arg Asn Ala

420 425 430

aac tgc ttc ggg ctg aaa cgc aaa ccg tgg gtc aaa acc tcg ttt gcc 1344

Asn Cys Phe Gly Leu Lys Arg Lys Pro Trp Val Lys Thr Ser Phe Ala

435 440 445

ccc ggt tcg aaa gtg gcg gaa att tat ttg aaa gaa gca ggc ctg ctg 1392

Pro Gly Ser Lys Val Ala Glu Ile Tyr Leu Lys Glu Ala Gly Leu Leu

450 455 460

ccc gaa atg gaa aaa ctc ggc ttc ggt atc gtc gcc ttc gcc tgc acc 1440

Pro Glu Met Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr

465 470 475 480

acc tgc aac ggc atg agt ggc gcg ctg gat ccg aaa atc cag aaa gaa 1488

Thr Cys Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Lys Glu

485 490 495

atc atc gac cgc gat ttg tac gcc acc gcc gta tta tca ggc aac cgc 1536

Ile Ile Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg

500 505 510

aac ttc gac ggc cgt gtc cat ccg tat gcg aaa cag gct ttc ctc gct 1584

Asn Phe Asp Gly Arg Val His Pro Tyr Ala Lys Gln Ala Phe Leu Ala

515 520 525

tcg cct ccg ttg gtc gtt gcc tac gcg ctg gca ggc agt atc cgt ttc 1632

Ser Pro Pro Leu Val Val Ala Tyr Ala Leu Ala Gly Ser Ile Arg Phe

530 535 540

gat att gaa aac gac gta ctc ggc gtt gca gac ggc aag gaa atc cgc 1680

Asp Ile Glu Asn Asp Val Leu Gly Val Ala Asp Gly Lys Glu Ile Arg

545 550 555 560

ctg aaa gac att tgg cct gcc gat gaa gaa atc gat gcc gtc gtt gcc 1728

Leu Lys Asp Ile Trp Pro Ala Asp Glu Glu Ile Asp Ala Val Val Ala

565 570 575

gaa tat gtg aaa ccg cag cag ttc cgc gat gtg tat gta ccg atg ttc 1776

Glu Tyr Val Lys Pro Gln Gln Phe Arg Asp Val Tyr Val Pro Met Phe

580 585 590

gac acc ggc aca gcg caa aaa gca cct agt ccg ctg tac gat tgg cgt 1824

Asp Thr Gly Thr Ala Gln Lys Ala Pro Ser Pro Leu Tyr Asp Trp Arg

595 600 605

ccg atg tcc acc tac atc cgc cgt ccg cct tac tgg gaa ggc gcg ctg 1872

Pro Met Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu

610 615 620

gca ggg gaa cgc aca tta aga ggt atg cgt ccg ctg gcg att ttg ccc 1920

Ala Gly Glu Arg Thr Leu Arg Gly Met Arg Pro Leu Ala Ile Leu Pro

625 630 635 640

gac aac atc acc acc gac cac ctc tcg ccg tcc aat gcg att ttg gcc 1968

Asp Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Leu Ala

645 650 655

gtc agt gcc gca ggc gag tat ttg gcg aaa atg ggt ttg cct gaa gaa 2016

Val Ser Ala Ala Gly Glu Tyr Leu Ala Lys Met Gly Leu Pro Glu Glu

660 665 670

gac ttc aac tct tac gca acc cac cgc ggc gac cac ttg acc gcc caa 2064

Asp Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln

675 680 685

cgc gct acc ttc gcc aat ccg aaa ctg ttt aac gaa atg gtg aaa aac 2112

Arg Ala Thr Phe Ala Asn Pro Lys Leu Phe Asn Glu Met Val Lys Asn

690 695 700

gaa gac ggc agc gtg cgc caa ggc tcg ttc gcc cgc gtc gaa ccc gaa 2160

Glu Asp Gly Ser Val Arg Gln Gly Ser Phe Ala Arg Val Glu Pro Glu

705 710 715 720

ggc gaa acc atg cgc atg tgg gaa gcc atc gaa acc tat atg aac cgc 2208

Gly Glu Thr Met Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asn Arg

725 730 735

aaa cag ccg ctc atc atc att gcc ggt gcg gac tat ggt caa ggc tca 2256

Lys Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser

740 745 750

agc cgc gac tgg gct gca aaa ggc gta cgc ctc gcc ggc gta gaa gcg 2304

Ser Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Ala

755 760 765

att gtt gcc gaa ggc ttc gag cgt atc cac cgc acc aac ctt atc ggc 2352

Ile Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly

770 775 780

atg ggc gtg ttg ccg ctg cag ttc aaa ccc gac acc aac cgc cat acc 2400

Met Gly Val Leu Pro Leu Gln Phe Lys Pro Asp Thr Asn Arg His Thr

785 790 795 800

ctg caa ctg gac ggt acg gaa acc tac gac gtg gtc ggc gaa cgc aca 2448

Leu Gln Leu Asp Gly Thr Glu Thr Tyr Asp Val Val Gly Glu Arg Thr

805 810 815

ccg cgc tgc gac ctg acc ctc gtg att cac cgt aaa aac ggc gaa acc 2496

Pro Arg Cys Asp Leu Thr Leu Val Ile His Arg Lys Asn Gly Glu Thr

820 825 830

gtc gaa gtt ccc gtt acc tgc cgc ctc gat act gca gaa gaa gta ttg 2544

Val Glu Val Pro Val Thr Cys Arg Leu Asp Thr Ala Glu Glu Val Leu

835 840 845

gta tat gaa gcc ggc ggc gtg ttg caa cgg ttt gca cag gat ttt ttg 2592

Val Tyr Glu Ala Gly Gly Val Leu Gln Arg Phe Ala Gln Asp Phe Leu

850 855 860

gaa ggg aac gcg gct tag 2610

Glu Gly Asn Ala Ala

865

<210> SEQ ID NO 42

<211> LENGTH: 869

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 42

Met Ala Ala Asn Gln Arg Tyr Arg Lys Pro Leu Pro Gly Thr Asp Leu

1 5 10 15

Glu Tyr Tyr Asp Ala Arg Ala Ala Cys Glu Asp Ile Lys Pro Gly Ser

20 25 30

Tyr Asp Lys Leu Pro Tyr Thr Ser Arg Ile Leu Ala Glu Asn Leu Val

35 40 45

Asn Arg Ala Asp Lys Val Asp Leu Pro Thr Leu Gln Ser Trp Leu Gly

50 55 60

Gln Leu Ile Glu Gly Lys Gln Glu Ile Asp Phe Pro Trp Tyr Pro Ala

65 70 75 80

Arg Val Val Cys His Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu

85 90 95

Ala Gly Leu Arg Asp Ala Ile Ala Glu Lys Gly Gly Asp Pro Ala Lys

100 105 110

Val Asn Pro Val Val Ala Lys Pro Ser Phe Ile Val Asp His Ser Leu

115 120 125

Ala Val Glu Cys Gly Gly Tyr Asp Pro Asp Ala Phe Arg Lys Asn Arg

130 135 140

Gln Ile Glu Asp Arg Arg Asn Glu Asp Arg Phe His Phe Ile Asn Trp

145 150 155 160

Thr Lys Thr Ala Phe Glu Asn Val Asp Val Ile Pro Ala Gly Asn Gly

165 170 175

Ile Met His Gln Ile Asn Leu Glu Lys Met Ser Pro Val Val Gln Val

180 185 190

Lys Asn Gly Val Ala Phe Pro Asp Thr Cys Val Gly Thr Asp Ser His

195 200 205

Thr Pro His Val Asp Ala Leu Gly Val Ile Ser Val Gly Val Gly Gly

210 215 220

Leu Glu Ala Glu Thr Val Met Leu Gly Arg Ala Ser Met Met Arg Leu

225 230 235 240

Pro Asp Ile Val Gly Val Glu Leu Asn Gly Lys Arg Gln Ala Gly Ile

245 250 255

Thr Ala Thr Asp Ile Val Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu

260 265 270

Arg Val Val Gly Ala Phe Val Glu Phe Phe Gly Glu Gly Ala Arg Ser

275 280 285

Leu Ser Ile Gly Asp Arg Ala Thr Ile Ser Asn Met Thr Pro Glu Phe

290 295 300

Gly Ala Thr Ala Ala Met Phe Ala Ile Asp Glu Gln Thr Ile Asp Tyr

305 310 315 320

Leu Lys Leu Thr Gly Arg Asp Asp Ala Gln Val Lys Leu Val Glu Thr

325 330 335

Tyr Ala Lys Thr Ala Gly Leu Trp Ala Asp Ala Leu Lys Thr Ala Val

340 345 350

Tyr Pro Arg Val Leu Lys Phe Asp Leu Ser Ser Val Thr Arg Asn Met

355 360 365

Ala Gly Pro Ser Asn Pro His Ala Arg Phe Ala Thr Ala Asp Leu Ala

370 375 380

Ser Lys Gly Leu Ala Lys Pro Tyr Glu Glu Pro Ser Asp Gly Gln Met

385 390 395 400

Pro Asp Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr

405 410 415

Ser Asn Pro Arg Asn Val Val Ala Ala Ala Leu Leu Ala Arg Asn Ala

420 425 430

Asn Cys Phe Gly Leu Lys Arg Lys Pro Trp Val Lys Thr Ser Phe Ala

435 440 445

Pro Gly Ser Lys Val Ala Glu Ile Tyr Leu Lys Glu Ala Gly Leu Leu

450 455 460

Pro Glu Met Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr

465 470 475 480

Thr Cys Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Lys Glu

485 490 495

Ile Ile Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg

500 505 510

Asn Phe Asp Gly Arg Val His Pro Tyr Ala Lys Gln Ala Phe Leu Ala

515 520 525

Ser Pro Pro Leu Val Val Ala Tyr Ala Leu Ala Gly Ser Ile Arg Phe

530 535 540

Asp Ile Glu Asn Asp Val Leu Gly Val Ala Asp Gly Lys Glu Ile Arg

545 550 555 560

Leu Lys Asp Ile Trp Pro Ala Asp Glu Glu Ile Asp Ala Val Val Ala

565 570 575

Glu Tyr Val Lys Pro Gln Gln Phe Arg Asp Val Tyr Val Pro Met Phe

580 585 590

Asp Thr Gly Thr Ala Gln Lys Ala Pro Ser Pro Leu Tyr Asp Trp Arg

595 600 605

Pro Met Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu

610 615 620

Ala Gly Glu Arg Thr Leu Arg Gly Met Arg Pro Leu Ala Ile Leu Pro

625 630 635 640

Asp Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Leu Ala

645 650 655

Val Ser Ala Ala Gly Glu Tyr Leu Ala Lys Met Gly Leu Pro Glu Glu

660 665 670

Asp Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln

675 680 685

Arg Ala Thr Phe Ala Asn Pro Lys Leu Phe Asn Glu Met Val Lys Asn

690 695 700

Glu Asp Gly Ser Val Arg Gln Gly Ser Phe Ala Arg Val Glu Pro Glu

705 710 715 720

Gly Glu Thr Met Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asn Arg

725 730 735

Lys Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser

740 745 750

Ser Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Ala

755 760 765

Ile Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly

770 775 780

Met Gly Val Leu Pro Leu Gln Phe Lys Pro Asp Thr Asn Arg His Thr

785 790 795 800

Leu Gln Leu Asp Gly Thr Glu Thr Tyr Asp Val Val Gly Glu Arg Thr

805 810 815

Pro Arg Cys Asp Leu Thr Leu Val Ile His Arg Lys Asn Gly Glu Thr

820 825 830

Val Glu Val Pro Val Thr Cys Arg Leu Asp Thr Ala Glu Glu Val Leu

835 840 845

Val Tyr Glu Ala Gly Gly Val Leu Gln Arg Phe Ala Gln Asp Phe Leu

850 855 860

Glu Gly Asn Ala Ala

865

<210> SEQ ID NO 43

<211> LENGTH: 1170

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1167)

<400> SEQUENCE: 43

atg ccg caa att aaa att ccc gcc gtt tac tac cgt ggc ggt aca tca 48

Met Pro Gln Ile Lys Ile Pro Ala Val Tyr Tyr Arg Gly Gly Thr Ser

1 5 10 15

aaa ggc gtg ttt ttc aaa cgt tcc gac ctg ccc gag gcg gcg cgg gaa 96

Lys Gly Val Phe Phe Lys Arg Ser Asp Leu Pro Glu Ala Ala Arg Glu

20 25 30

gcg gga agc gca cgc gac aaa atc ctc ttg cgc gta ctc ggc agc ccg 144

Ala Gly Ser Ala Arg Asp Lys Ile Leu Leu Arg Val Leu Gly Ser Pro

35 40 45

gac ccc tac ggc aag cag ata gac ggt ttg ggc aac gcc agt tcg tcc 192

Asp Pro Tyr Gly Lys Gln Ile Asp Gly Leu Gly Asn Ala Ser Ser Ser

50 55 60

acc agc aaa gcc gtg att ttg gac aag tcc gaa cgc acc gat cac gat 240

Thr Ser Lys Ala Val Ile Leu Asp Lys Ser Glu Arg Thr Asp His Asp

65 70 75 80

gtc gat tac ctt ttc ggg caa gtt tcc atc gac aaa cct ttt gtc gat 288

Val Asp Tyr Leu Phe Gly Gln Val Ser Ile Asp Lys Pro Phe Val Asp

85 90 95

tgg agt ggc aac tgc ggc aac ctc acc gcc gcc gtg ggc gca ttt gcc 336

Trp Ser Gly Asn Cys Gly Asn Leu Thr Ala Ala Val Gly Ala Phe Ala

100 105 110

atc gag caa ggc ttg gtc gat aaa tcc aaa atc cct tca gac ggc ccg 384

Ile Glu Gln Gly Leu Val Asp Lys Ser Lys Ile Pro Ser Asp Gly Pro

115 120 125

tgt acc gtc aaa atc tgg cag aaa aac atc ggc aaa acc att att gcc 432

Cys Thr Val Lys Ile Trp Gln Lys Asn Ile Gly Lys Thr Ile Ile Ala

130 135 140

cat gta ccg atg caa aac ggc gca gtt ttg gaa aca ggc gat ttt gag 480

His Val Pro Met Gln Asn Gly Ala Val Leu Glu Thr Gly Asp Phe Glu

145 150 155 160

ctc gac ggc gta acg ttc ccg gca gcc gaa gta caa atc gaa ttt ctt 528

Leu Asp Gly Val Thr Phe Pro Ala Ala Glu Val Gln Ile Glu Phe Leu

165 170 175

gat cca gcc gac ggc gaa ggc agt atg ttc cca acc ggc aat ttg gtc 576

Asp Pro Ala Asp Gly Glu Gly Ser Met Phe Pro Thr Gly Asn Leu Val

180 185 190

gat gaa att gat gtg ccg aat ata ggc cgt ttg aaa gcc acg ctc atc 624

Asp Glu Ile Asp Val Pro Asn Ile Gly Arg Leu Lys Ala Thr Leu Ile

195 200 205

aac gcg ggc att ccg acc gtt ttc ctg aat gcc gcc gac ttg ggc tac 672

Asn Ala Gly Ile Pro Thr Val Phe Leu Asn Ala Ala Asp Leu Gly Tyr

210 215 220

acg ggc aaa gag ttg caa gac gac atc aac aac gat gcc gca gct ttg 720

Thr Gly Lys Glu Leu Gln Asp Asp Ile Asn Asn Asp Ala Ala Ala Leu

225 230 235 240

gaa aaa ttc gag aaa atc cgc gct tac ggt gcg ctg aaa atg ggt cta 768

Glu Lys Phe Glu Lys Ile Arg Ala Tyr Gly Ala Leu Lys Met Gly Leu

245 250 255

atc agc gac gta tcc gaa gct gcc gcc cgc gcg cac acg ccg aaa gtc 816

Ile Ser Asp Val Ser Glu Ala Ala Ala Arg Ala His Thr Pro Lys Val

260 265 270

gcc ttc gtc gcg ccc gcc gcc gat tac acc gcc tcc agt ggc aaa acc 864

Ala Phe Val Ala Pro Ala Ala Asp Tyr Thr Ala Ser Ser Gly Lys Thr

275 280 285

gtg aat gcc gcc gac atc gat ttg ctg gta cgc gcc ctg agc atg ggc 912

Val Asn Ala Ala Asp Ile Asp Leu Leu Val Arg Ala Leu Ser Met Gly

290 295 300

aaa ttg cac cac gcg atg atg ggt acc gcc tct gtt gcc att gcg acc 960

Lys Leu His His Ala Met Met Gly Thr Ala Ser Val Ala Ile Ala Thr

305 310 315 320

gcc gcc gcc gtg ccc ggt acg ctg gtc aac ctt gcc gca ggg gcg gga 1008

Ala Ala Ala Val Pro Gly Thr Leu Val Asn Leu Ala Ala Gly Ala Gly

325 330 335

acg cgt aaa gaa gtg cgc ttc ggg cat cct tcc ggc aca ttg cgc gtc 1056

Thr Arg Lys Glu Val Arg Phe Gly His Pro Ser Gly Thr Leu Arg Val

340 345 350

ggt gca gcc gcc gaa tgt cag gac gga caa tgg acg gcc acc aaa gcg 1104

Gly Ala Ala Ala Glu Cys Gln Asp Gly Gln Trp Thr Ala Thr Lys Ala

355 360 365

gtt atg agc cgc agc gca cgc gtg atg atg gaa ggt tgg gtc agg gtg 1152

Val Met Ser Arg Ser Ala Arg Val Met Met Glu Gly Trp Val Arg Val

370 375 380

ccg gaa gat tgt ttt taa 1170

Pro Glu Asp Cys Phe

385

<210> SEQ ID NO 44

<211> LENGTH: 389

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 44

Met Pro Gln Ile Lys Ile Pro Ala Val Tyr Tyr Arg Gly Gly Thr Ser

1 5 10 15

Lys Gly Val Phe Phe Lys Arg Ser Asp Leu Pro Glu Ala Ala Arg Glu

20 25 30

Ala Gly Ser Ala Arg Asp Lys Ile Leu Leu Arg Val Leu Gly Ser Pro

35 40 45

Asp Pro Tyr Gly Lys Gln Ile Asp Gly Leu Gly Asn Ala Ser Ser Ser

50 55 60

Thr Ser Lys Ala Val Ile Leu Asp Lys Ser Glu Arg Thr Asp His Asp

65 70 75 80

Val Asp Tyr Leu Phe Gly Gln Val Ser Ile Asp Lys Pro Phe Val Asp

85 90 95

Trp Ser Gly Asn Cys Gly Asn Leu Thr Ala Ala Val Gly Ala Phe Ala

100 105 110

Ile Glu Gln Gly Leu Val Asp Lys Ser Lys Ile Pro Ser Asp Gly Pro

115 120 125

Cys Thr Val Lys Ile Trp Gln Lys Asn Ile Gly Lys Thr Ile Ile Ala

130 135 140

His Val Pro Met Gln Asn Gly Ala Val Leu Glu Thr Gly Asp Phe Glu

145 150 155 160

Leu Asp Gly Val Thr Phe Pro Ala Ala Glu Val Gln Ile Glu Phe Leu

165 170 175

Asp Pro Ala Asp Gly Glu Gly Ser Met Phe Pro Thr Gly Asn Leu Val

180 185 190

Asp Glu Ile Asp Val Pro Asn Ile Gly Arg Leu Lys Ala Thr Leu Ile

195 200 205

Asn Ala Gly Ile Pro Thr Val Phe Leu Asn Ala Ala Asp Leu Gly Tyr

210 215 220

Thr Gly Lys Glu Leu Gln Asp Asp Ile Asn Asn Asp Ala Ala Ala Leu

225 230 235 240

Glu Lys Phe Glu Lys Ile Arg Ala Tyr Gly Ala Leu Lys Met Gly Leu

245 250 255

Ile Ser Asp Val Ser Glu Ala Ala Ala Arg Ala His Thr Pro Lys Val

260 265 270

Ala Phe Val Ala Pro Ala Ala Asp Tyr Thr Ala Ser Ser Gly Lys Thr

275 280 285

Val Asn Ala Ala Asp Ile Asp Leu Leu Val Arg Ala Leu Ser Met Gly

290 295 300

Lys Leu His His Ala Met Met Gly Thr Ala Ser Val Ala Ile Ala Thr

305 310 315 320

Ala Ala Ala Val Pro Gly Thr Leu Val Asn Leu Ala Ala Gly Ala Gly

325 330 335

Thr Arg Lys Glu Val Arg Phe Gly His Pro Ser Gly Thr Leu Arg Val

340 345 350

Gly Ala Ala Ala Glu Cys Gln Asp Gly Gln Trp Thr Ala Thr Lys Ala

355 360 365

Val Met Ser Arg Ser Ala Arg Val Met Met Glu Gly Trp Val Arg Val

370 375 380

Pro Glu Asp Cys Phe

385

<210> SEQ ID NO 45

<211> LENGTH: 954

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(951)

<400> SEQUENCE: 45

atg cgc acg ccg ttt tgt tgg gca tac gcc aat gcc gcc cga ata tcg 48

Met Arg Thr Pro Phe Cys Trp Ala Tyr Ala Asn Ala Ala Arg Ile Ser

1 5 10 15

gca atg ctg ccg gcg tgt tgg gcg cag gcg atg ttg gcc gaa gta atc 96

Ala Met Leu Pro Ala Cys Trp Ala Gln Ala Met Leu Ala Glu Val Ile

20 25 30

agc tgc aac aag gct tcg tcg ctg ccg cag cct tcg gcg aga tcg gcg 144

Ser Cys Asn Lys Ala Ser Ser Leu Pro Gln Pro Ser Ala Arg Ser Ala

35 40 45

ttt aaa tca acc tgc ttc atg ggt gat tct ccg tat ttg gtt cag ata 192

Phe Lys Ser Thr Cys Phe Met Gly Asp Ser Pro Tyr Leu Val Gln Ile

50 55 60

gac ttg gtt ttt gcg ccg cag ggc ggt ggc ttc ttt caa gcc gat tat 240

Asp Leu Val Phe Ala Pro Gln Gly Gly Gly Phe Phe Gln Ala Asp Tyr

65 70 75 80

ttt gaa ttt gac ttt gct gcc gaa gcg cac ctg tgc cag cct gcc caa 288

Phe Glu Phe Asp Phe Ala Ala Glu Ala His Leu Cys Gln Pro Ala Gln

85 90 95

atc ggc ggc ggc aac ggt agc gat ttt cgg ata acc gcc ggt ggt ttg 336

Ile Gly Gly Gly Asn Gly Ser Asp Phe Arg Ile Thr Ala Gly Gly Leu

100 105 110

cgc atc ggc cag cag gat aat cgg ttt gcc gcc ggg cgg cac ctg cac 384

Arg Ile Gly Gln Gln Asp Asn Arg Phe Ala Ala Gly Arg His Leu His

115 120 125

ggt tcc tgc ctg aac agc gtg gga cag cat ttc caa agg ttg cga cag 432

Gly Ser Cys Leu Asn Ser Val Gly Gln His Phe Gln Arg Leu Arg Gln

130 135 140

ggt cag cgg ctg tcc gtc gaa gcg gta gcc cat gcg gtt gct atc gct 480

Gly Gln Arg Leu Ser Val Glu Ala Val Ala His Ala Val Ala Ile Ala

145 150 155 160

ttg cag cgt cca cgt ttc ccg ttc cag att cag acg ccc ttt ttc act 528

Leu Gln Arg Pro Arg Phe Pro Phe Gln Ile Gln Thr Pro Phe Phe Thr

165 170 175

gaa agc ggc ata ttc cga cga agg aac aag gtg gat ggt atc ggt aaa 576

Glu Ser Gly Ile Phe Arg Arg Arg Asn Lys Val Asp Gly Ile Gly Lys

180 185 190

cgg tat cgg ggc aat gcc gac ttt gga caa ttc ctg cgc acc ttt gcc 624

Arg Tyr Arg Gly Asn Ala Asp Phe Gly Gln Phe Leu Arg Thr Phe Ala

195 200 205

gat ggg gag ata atc gcc ttt ttg cag cat tct gcc ctg atg gcc gcc 672

Asp Gly Glu Ile Ile Ala Phe Leu Gln His Ser Ala Leu Met Ala Ala

210 215 220

gaa acc ggc ttt cag gtc ggt gct tct cga acc cat cac ttc cgg cac 720

Glu Thr Gly Phe Gln Val Gly Ala Ser Arg Thr His His Phe Arg His

225 230 235 240

atc aaa tcc gcc cgc cac gca cac ata gcc gta cat gcc ctg cac ggc 768

Ile Lys Ser Ala Arg His Ala His Ile Ala Val His Ala Leu His Gly

245 250 255

acg cac cat ttt caa ggt ctg ccc ttt gcg ggc ggt ata acg cca ata 816

Thr His His Phe Gln Gly Leu Pro Phe Ala Gly Gly Ile Thr Pro Ile

260 265 270

cga ata gac cgg ttc gcc gtc caa ttc cgc ctg ata cac ggc acc ggt 864

Arg Ile Asp Arg Phe Ala Val Gln Phe Arg Leu Ile His Gly Thr Gly

275 280 285

gag aca aaa cgg cgt atc ccg ttc aaa cac cag cat tat ccc gcc caa 912

Glu Thr Lys Arg Arg Ile Pro Phe Lys His Gln His Tyr Pro Ala Gln

290 295 300

agc gat ttc gat tgc ggc cgt gcc ttc gtc gtt gcc caa taa 954

Ser Asp Phe Asp Cys Gly Arg Ala Phe Val Val Ala Gln

305 310 315

<210> SEQ ID NO 46

<211> LENGTH: 317

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 46

Met Arg Thr Pro Phe Cys Trp Ala Tyr Ala Asn Ala Ala Arg Ile Ser

1 5 10 15

Ala Met Leu Pro Ala Cys Trp Ala Gln Ala Met Leu Ala Glu Val Ile

20 25 30

Ser Cys Asn Lys Ala Ser Ser Leu Pro Gln Pro Ser Ala Arg Ser Ala

35 40 45

Phe Lys Ser Thr Cys Phe Met Gly Asp Ser Pro Tyr Leu Val Gln Ile

50 55 60

Asp Leu Val Phe Ala Pro Gln Gly Gly Gly Phe Phe Gln Ala Asp Tyr

65 70 75 80

Phe Glu Phe Asp Phe Ala Ala Glu Ala His Leu Cys Gln Pro Ala Gln

85 90 95

Ile Gly Gly Gly Asn Gly Ser Asp Phe Arg Ile Thr Ala Gly Gly Leu

100 105 110

Arg Ile Gly Gln Gln Asp Asn Arg Phe Ala Ala Gly Arg His Leu His

115 120 125

Gly Ser Cys Leu Asn Ser Val Gly Gln His Phe Gln Arg Leu Arg Gln

130 135 140

Gly Gln Arg Leu Ser Val Glu Ala Val Ala His Ala Val Ala Ile Ala

145 150 155 160

Leu Gln Arg Pro Arg Phe Pro Phe Gln Ile Gln Thr Pro Phe Phe Thr

165 170 175

Glu Ser Gly Ile Phe Arg Arg Arg Asn Lys Val Asp Gly Ile Gly Lys

180 185 190

Arg Tyr Arg Gly Asn Ala Asp Phe Gly Gln Phe Leu Arg Thr Phe Ala

195 200 205

Asp Gly Glu Ile Ile Ala Phe Leu Gln His Ser Ala Leu Met Ala Ala

210 215 220

Glu Thr Gly Phe Gln Val Gly Ala Ser Arg Thr His His Phe Arg His

225 230 235 240

Ile Lys Ser Ala Arg His Ala His Ile Ala Val His Ala Leu His Gly

245 250 255

Thr His His Phe Gln Gly Leu Pro Phe Ala Gly Gly Ile Thr Pro Ile

260 265 270

Arg Ile Asp Arg Phe Ala Val Gln Phe Arg Leu Ile His Gly Thr Gly

275 280 285

Glu Thr Lys Arg Arg Ile Pro Phe Lys His Gln His Tyr Pro Ala Gln

290 295 300

Ser Asp Phe Asp Cys Gly Arg Ala Phe Val Val Ala Gln

305 310 315

<210> SEQ ID NO 47

<211> LENGTH: 648

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(645)

<400> SEQUENCE: 47

atg aga ata gag atc aca cca atc agc gaa tcc gct ttg gtc tgc cga 48

Met Arg Ile Glu Ile Thr Pro Ile Ser Glu Ser Ala Leu Val Cys Arg

1 5 10 15

ctg aat gcg cct tcc gaa ctg ggc aaa cag caa aag ttg tgg gcg ttt 96

Leu Asn Ala Pro Ser Glu Leu Gly Lys Gln Gln Lys Leu Trp Ala Phe

20 25 30

gcc gct gcg ctc ggg cag cac gac agg att gag gaa gtg gtg gtc ggc 144

Ala Ala Ala Leu Gly Gln His Asp Arg Ile Glu Glu Val Val Val Gly

35 40 45

atg aac aat ctg acc gtg ttc acc cgt ttc gat acc gat ttg gcg acg 192

Met Asn Asn Leu Thr Val Phe Thr Arg Phe Asp Thr Asp Leu Ala Thr

50 55 60

ctt gcc gat gaa ttg caa tat gtg tgg gaa cac acc gcc gtt aca gac 240

Leu Ala Asp Glu Leu Gln Tyr Val Trp Glu His Thr Ala Val Thr Asp

65 70 75 80

cat cag ggc aaa ctg gtg gaa att ccc gtc tgc tac ggc ggc gaa tac 288

His Gln Gly Lys Leu Val Glu Ile Pro Val Cys Tyr Gly Gly Glu Tyr

85 90 95

ggc ccg gat ttg gcg gaa gtc gct gct ttc cat cag acg gtt att tcc 336

Gly Pro Asp Leu Ala Glu Val Ala Ala Phe His Gln Thr Val Ile Ser

100 105 110

gaa atc gtc cgc cgc cat acg gcg caa act tat acc gta ttt atg atg 384

Glu Ile Val Arg Arg His Thr Ala Gln Thr Tyr Thr Val Phe Met Met

115 120 125

ggc ttc cag cct ggt ttc cct tat ctg ggc ggc ttg ccc gaa gca ttg 432

Gly Phe Gln Pro Gly Phe Pro Tyr Leu Gly Gly Leu Pro Glu Ala Leu

130 135 140

cac acg ccc cgc cgt gcc gtg ccg aga acg tcc gtt cct gcc ggt tcg 480

His Thr Pro Arg Arg Ala Val Pro Arg Thr Ser Val Pro Ala Gly Ser

145 150 155 160

gtc ggt atc ggc ggc agt cag acc ggt gtg tat ccg ttc gct tcg ccc 528

Val Gly Ile Gly Gly Ser Gln Thr Gly Val Tyr Pro Phe Ala Ser Pro

165 170 175

ggc ggc tgg cag att atc ggc aga acc gaa tta ccc ttg ttc cga gcc 576

Gly Gly Trp Gln Ile Ile Gly Arg Thr Glu Leu Pro Leu Phe Arg Ala

180 185 190

gat ttg aat ccg ccg acc ctg ctg gcg gcg ggt gac caa gtc cgc ttt 624

Asp Leu Asn Pro Pro Thr Leu Leu Ala Ala Gly Asp Gln Val Arg Phe

195 200 205

gtt gca gaa agg att gag cca tga 648

Val Ala Glu Arg Ile Glu Pro

210 215

<210> SEQ ID NO 48

<211> LENGTH: 215

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 48

Met Arg Ile Glu Ile Thr Pro Ile Ser Glu Ser Ala Leu Val Cys Arg

1 5 10 15

Leu Asn Ala Pro Ser Glu Leu Gly Lys Gln Gln Lys Leu Trp Ala Phe

20 25 30

Ala Ala Ala Leu Gly Gln His Asp Arg Ile Glu Glu Val Val Val Gly

35 40 45

Met Asn Asn Leu Thr Val Phe Thr Arg Phe Asp Thr Asp Leu Ala Thr

50 55 60

Leu Ala Asp Glu Leu Gln Tyr Val Trp Glu His Thr Ala Val Thr Asp

65 70 75 80

His Gln Gly Lys Leu Val Glu Ile Pro Val Cys Tyr Gly Gly Glu Tyr

85 90 95

Gly Pro Asp Leu Ala Glu Val Ala Ala Phe His Gln Thr Val Ile Ser

100 105 110

Glu Ile Val Arg Arg His Thr Ala Gln Thr Tyr Thr Val Phe Met Met

115 120 125

Gly Phe Gln Pro Gly Phe Pro Tyr Leu Gly Gly Leu Pro Glu Ala Leu

130 135 140

His Thr Pro Arg Arg Ala Val Pro Arg Thr Ser Val Pro Ala Gly Ser

145 150 155 160

Val Gly Ile Gly Gly Ser Gln Thr Gly Val Tyr Pro Phe Ala Ser Pro

165 170 175

Gly Gly Trp Gln Ile Ile Gly Arg Thr Glu Leu Pro Leu Phe Arg Ala

180 185 190

Asp Leu Asn Pro Pro Thr Leu Leu Ala Ala Gly Asp Gln Val Arg Phe

195 200 205

Val Ala Glu Arg Ile Glu Pro

210 215

<210> SEQ ID NO 49

<211> LENGTH: 930

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(927)

<400> SEQUENCE: 49

atg att cac gtt tcg gca gtg cag gca ccg gcg cat att cag gat acc 48

Met Ile His Val Ser Ala Val Gln Ala Pro Ala His Ile Gln Asp Thr

1 5 10 15

gga cgc tac gga cac cgg cgt tac ggc atc ggt cat gcc ggt gcg atg 96

Gly Arg Tyr Gly His Arg Arg Tyr Gly Ile Gly His Ala Gly Ala Met

20 25 30

gac acg gtt gct ttg gcg gcg ggt aat att tta ttg ggc aac gac gaa 144

Asp Thr Val Ala Leu Ala Ala Gly Asn Ile Leu Leu Gly Asn Asp Glu

35 40 45

ggc acg gcc gca atc gaa atc gct ttg ggc ggg ata atg ctg gtg ttt 192

Gly Thr Ala Ala Ile Glu Ile Ala Leu Gly Gly Ile Met Leu Val Phe

50 55 60

gaa cgg gat acg ccg ttt tgt ctc acc ggt gcc gtg tat cag gcg gaa 240

Glu Arg Asp Thr Pro Phe Cys Leu Thr Gly Ala Val Tyr Gln Ala Glu

65 70 75 80

ttg gac ggc gaa ccg gtc tat tcg tat tgg cgt tat acc gcc cgc aaa 288

Leu Asp Gly Glu Pro Val Tyr Ser Tyr Trp Arg Tyr Thr Ala Arg Lys

85 90 95

ggg cag acc ttg aaa atg gtg cgt gcc gtg cag ggc atg tac ggc tat 336

Gly Gln Thr Leu Lys Met Val Arg Ala Val Gln Gly Met Tyr Gly Tyr

100 105 110

gtg tgc gtg gcg ggc gga ttt gat gtg ccg gaa gtg atg ggt tcg aga 384

Val Cys Val Ala Gly Gly Phe Asp Val Pro Glu Val Met Gly Ser Arg

115 120 125

agc acc gac ctg aaa gcc ggt ttc ggc ggc cat cag ggc aga atg ctg 432

Ser Thr Asp Leu Lys Ala Gly Phe Gly Gly His Gln Gly Arg Met Leu

130 135 140

caa aaa ggc gat tat ctc ccc atc ggc aaa ggt gcg cag gaa ttg tcc 480

Gln Lys Gly Asp Tyr Leu Pro Ile Gly Lys Gly Ala Gln Glu Leu Ser

145 150 155 160

aaa gtc ggc att gcc ccg ata ccg ttt acc gat acc atc cac ctt gtt 528

Lys Val Gly Ile Ala Pro Ile Pro Phe Thr Asp Thr Ile His Leu Val

165 170 175

cct tcg tcg gaa tat gcc gct ttc agt gaa aaa ggg cgt ctg aat ctg 576

Pro Ser Ser Glu Tyr Ala Ala Phe Ser Glu Lys Gly Arg Leu Asn Leu

180 185 190

gaa cgg gaa acg tgg acg ctg caa agc gat agc aac cgc atg ggc tac 624

Glu Arg Glu Thr Trp Thr Leu Gln Ser Asp Ser Asn Arg Met Gly Tyr

195 200 205

cgc ttc gac gga cag ccg ctg acc ctg tcg caa cct ttg gaa atg ctg 672

Arg Phe Asp Gly Gln Pro Leu Thr Leu Ser Gln Pro Leu Glu Met Leu

210 215 220

tcc cac gct gtt cag gca gga acc gtg cag gtg ccg ccc ggc ggc aaa 720

Ser His Ala Val Gln Ala Gly Thr Val Gln Val Pro Pro Gly Gly Lys

225 230 235 240

ccg att atc ctg ctg gcc gat gcg caa acc acc ggc ggt tat ccg aaa 768

Pro Ile Ile Leu Leu Ala Asp Ala Gln Thr Thr Gly Gly Tyr Pro Lys

245 250 255

atc gct acc gtt gcc gcc gcc gat ttg ggc agg ctg gca cag gtg cgc 816

Ile Ala Thr Val Ala Ala Ala Asp Leu Gly Arg Leu Ala Gln Val Arg

260 265 270

ttc ggc agc aaa gtc aaa ttc aaa ata atc ggc ttg aaa gaa gcc acc 864

Phe Gly Ser Lys Val Lys Phe Lys Ile Ile Gly Leu Lys Glu Ala Thr

275 280 285

gcc ctg cgg cgc aaa aac caa gtc tat ctg aac caa ata cgg aga atc 912

Ala Leu Arg Arg Lys Asn Gln Val Tyr Leu Asn Gln Ile Arg Arg Ile

290 295 300

acc cat gaa gca ggt tga 930

Thr His Glu Ala Gly

305

<210> SEQ ID NO 50

<211> LENGTH: 309

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 50

Met Ile His Val Ser Ala Val Gln Ala Pro Ala His Ile Gln Asp Thr

1 5 10 15

Gly Arg Tyr Gly His Arg Arg Tyr Gly Ile Gly His Ala Gly Ala Met

20 25 30

Asp Thr Val Ala Leu Ala Ala Gly Asn Ile Leu Leu Gly Asn Asp Glu

35 40 45

Gly Thr Ala Ala Ile Glu Ile Ala Leu Gly Gly Ile Met Leu Val Phe

50 55 60

Glu Arg Asp Thr Pro Phe Cys Leu Thr Gly Ala Val Tyr Gln Ala Glu

65 70 75 80

Leu Asp Gly Glu Pro Val Tyr Ser Tyr Trp Arg Tyr Thr Ala Arg Lys

85 90 95

Gly Gln Thr Leu Lys Met Val Arg Ala Val Gln Gly Met Tyr Gly Tyr

100 105 110

Val Cys Val Ala Gly Gly Phe Asp Val Pro Glu Val Met Gly Ser Arg

115 120 125

Ser Thr Asp Leu Lys Ala Gly Phe Gly Gly His Gln Gly Arg Met Leu

130 135 140

Gln Lys Gly Asp Tyr Leu Pro Ile Gly Lys Gly Ala Gln Glu Leu Ser

145 150 155 160

Lys Val Gly Ile Ala Pro Ile Pro Phe Thr Asp Thr Ile His Leu Val

165 170 175

Pro Ser Ser Glu Tyr Ala Ala Phe Ser Glu Lys Gly Arg Leu Asn Leu

180 185 190

Glu Arg Glu Thr Trp Thr Leu Gln Ser Asp Ser Asn Arg Met Gly Tyr

195 200 205

Arg Phe Asp Gly Gln Pro Leu Thr Leu Ser Gln Pro Leu Glu Met Leu

210 215 220

Ser His Ala Val Gln Ala Gly Thr Val Gln Val Pro Pro Gly Gly Lys

225 230 235 240

Pro Ile Ile Leu Leu Ala Asp Ala Gln Thr Thr Gly Gly Tyr Pro Lys

245 250 255

Ile Ala Thr Val Ala Ala Ala Asp Leu Gly Arg Leu Ala Gln Val Arg

260 265 270

Phe Gly Ser Lys Val Lys Phe Lys Ile Ile Gly Leu Lys Glu Ala Thr

275 280 285

Ala Leu Arg Arg Lys Asn Gln Val Tyr Leu Asn Gln Ile Arg Arg Ile

290 295 300

Thr His Glu Ala Gly

305

<210> SEQ ID NO 51

<211> LENGTH: 2094

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(2091)

<400> SEQUENCE: 51

atg aat tcg acc gca agt aaa acc ctg aaa gga ttg tcg ctg gtg ttt 48

Met Asn Ser Thr Ala Ser Lys Thr Leu Lys Gly Leu Ser Leu Val Phe

1 5 10 15

ttc gcc tct gga ttc tgc gcc ctg att tac cag gtc agc tgg cag agg 96

Phe Ala Ser Gly Phe Cys Ala Leu Ile Tyr Gln Val Ser Trp Gln Arg

20 25 30

ctt cta ttc agt cac ata ggt atc gat ttg agt tcg att act gtc att 144

Leu Leu Phe Ser His Ile Gly Ile Asp Leu Ser Ser Ile Thr Val Ile

35 40 45

att tct gta ttt atg gtc ggc ttg ggt gta ggt gcg tat ttc ggt gga 192

Ile Ser Val Phe Met Val Gly Leu Gly Val Gly Ala Tyr Phe Gly Gly

50 55 60

cgc att gct gac cgt ttt cct tca agt atc atc ccc ctg ttt tgc atc 240

Arg Ile Ala Asp Arg Phe Pro Ser Ser Ile Ile Pro Leu Phe Cys Ile

65 70 75 80

gct gaa gta tcc atc ggt ctg ttc ggt ttg gta agc agg ggt ctg att 288

Ala Glu Val Ser Ile Gly Leu Phe Gly Leu Val Ser Arg Gly Leu Ile

85 90 95

tcc ggc ttg ggg cat ctt tta gtt gag gct gat ttg ccc atc atc gct 336

Ser Gly Leu Gly His Leu Leu Val Glu Ala Asp Leu Pro Ile Ile Ala

100 105 110

gct gcc aat ttc ctc tta ttg ctg ctt cct acc ttt atg atg ggc gcg 384

Ala Ala Asn Phe Leu Leu Leu Leu Leu Pro Thr Phe Met Met Gly Ala

115 120 125

acc ttg ccc ttg ctg acc tgt ttt ttt aac cgg aaa ata cat aat gtt 432

Thr Leu Pro Leu Leu Thr Cys Phe Phe Asn Arg Lys Ile His Asn Val

130 135 140

ggc gag tct atc ggt acc tta tat ttt ttc aac act ttg ggt gcg gca 480

Gly Glu Ser Ile Gly Thr Leu Tyr Phe Phe Asn Thr Leu Gly Ala Ala

145 150 155 160

ctc gga tcg ctt gcc gcc gcc gaa ttt ttc tac gtc ttt ttt acc ctc 528

Leu Gly Ser Leu Ala Ala Ala Glu Phe Phe Tyr Val Phe Phe Thr Leu

165 170 175

tcc caa acc att gcg ctg aca gcc tgc ttt aac ctt ctg att gct gct 576

Ser Gln Thr Ile Ala Leu Thr Ala Cys Phe Asn Leu Leu Ile Ala Ala

180 185 190

tca gta tgc tgc gtt aca gaa agg atg gat ata gtg aac act aaa ccg 624

Ser Val Cys Cys Val Thr Glu Arg Met Asp Ile Val Asn Thr Lys Pro

195 200 205

aat act agt ttg att tat atg ctt tct ttc ctt agc ggc tta ttg agc 672

Asn Thr Ser Leu Ile Tyr Met Leu Ser Phe Leu Ser Gly Leu Leu Ser

210 215 220

ttg ggt ata gaa gtc ttg tgg gta agg atg ttt tcg ttc gca gca cag 720

Leu Gly Ile Glu Val Leu Trp Val Arg Met Phe Ser Phe Ala Ala Gln

225 230 235 240

tcc gtg cct cag gca ttt tca ttt act ctt gcc tat ttt ctg acc ggt 768

Ser Val Pro Gln Ala Phe Ser Phe Thr Leu Ala Tyr Phe Leu Thr Gly

245 250 255

atc gcc gtc ggc gcg tat ttt ggc aaa cgg att tgc cgc agc cgc ttt 816

Ile Ala Val Gly Ala Tyr Phe Gly Lys Arg Ile Cys Arg Ser Arg Phe

260 265 270

gtt gat att ccc ttt atc ggg cag tgc ttc ttg tgg gcg ggt att gcc 864

Val Asp Ile Pro Phe Ile Gly Gln Cys Phe Leu Trp Ala Gly Ile Ala

275 280 285

gac ttt ttg att ttg ggt gct gcg tgg ttg ttg acg ggt ttt tcc ggc 912

Asp Phe Leu Ile Leu Gly Ala Ala Trp Leu Leu Thr Gly Phe Ser Gly

290 295 300

ttc gtc cac cac gcc ggt atc ttc att acc ctg tct gcc gtc gtc aga 960

Phe Val His His Ala Gly Ile Phe Ile Thr Leu Ser Ala Val Val Arg

305 310 315 320

ggg ttg att ttc ccg ctc gta cac cat gtg ggt acg gat ggc aac aaa 1008

Gly Leu Ile Phe Pro Leu Val His His Val Gly Thr Asp Gly Asn Lys

325 330 335

tcc gga cga cag gtt tcc aat gtt tat ttc gcc aac gtt gcc ggc agt 1056

Ser Gly Arg Gln Val Ser Asn Val Tyr Phe Ala Asn Val Ala Gly Ser

340 345 350

gca ttg ggt ccg gtc ctt atc ggc ttt gtg ata ctt gat ttc ttg tcc 1104

Ala Leu Gly Pro Val Leu Ile Gly Phe Val Ile Leu Asp Phe Leu Ser

355 360 365

acc caa cag att tac ctg ctc atc tgt ttg att tct gct gct gtc cct 1152

Thr Gln Gln Ile Tyr Leu Leu Ile Cys Leu Ile Ser Ala Ala Val Pro

370 375 380

ttg ttt tgt aca ctg ttc caa aaa agt ctc cga ctg aat gca gtg tcg 1200

Leu Phe Cys Thr Leu Phe Gln Lys Ser Leu Arg Leu Asn Ala Val Ser

385 390 395 400

gta gca gtt tcc cta atg ttc ggc atc ctc atg ttc cta ctg ccg gat 1248

Val Ala Val Ser Leu Met Phe Gly Ile Leu Met Phe Leu Leu Pro Asp

405 410 415

tct gtc ttt caa aat att gct gac cgt ccg gat cgg ctg att gaa aac 1296

Ser Val Phe Gln Asn Ile Ala Asp Arg Pro Asp Arg Leu Ile Glu Asn

420 425 430

aaa cac ggc att gtt gcg gtt tac cat aga gat ggt gat aag gtt gtt 1344

Lys His Gly Ile Val Ala Val Tyr His Arg Asp Gly Asp Lys Val Val

435 440 445

tat ggg gcg aat gta tac gac ggc gca tac aat acc gat gta ttc aat 1392

Tyr Gly Ala Asn Val Tyr Asp Gly Ala Tyr Asn Thr Asp Val Phe Asn

450 455 460

agt gtc aac ggc atc gaa cgt gcc tat ctg cta ccc tcc ctg aag tct 1440

Ser Val Asn Gly Ile Glu Arg Ala Tyr Leu Leu Pro Ser Leu Lys Ser

465 470 475 480

ggc ata cgc cgc att ttc gtc gtt gga ttg agt aca ggt tcg tgg gcg 1488

Gly Ile Arg Arg Ile Phe Val Val Gly Leu Ser Thr Gly Ser Trp Ala

485 490 495

cgc gtc ttg tct gcc att ccg gaa atg cag tcg atg atc gtt gcg gaa 1536

Arg Val Leu Ser Ala Ile Pro Glu Met Gln Ser Met Ile Val Ala Glu

500 505 510

atc aat ccg gca tac cgt agc ctt atc gcg gac gag ccg caa atc gcc 1584

Ile Asn Pro Ala Tyr Arg Ser Leu Ile Ala Asp Glu Pro Gln Ile Ala

515 520 525

ccg ctt ttg cag gac aaa cgt gtt gaa att gta ttg gat gac ggt agg 1632

Pro Leu Leu Gln Asp Lys Arg Val Glu Ile Val Leu Asp Asp Gly Arg

530 535 540

aaa tgg ctg cgt cgc cat cct gat gaa aaa ttc gac ctg att ttg atg 1680

Lys Trp Leu Arg Arg His Pro Asp Glu Lys Phe Asp Leu Ile Leu Met

545 550 555 560

aat acg act tgg tac tgg cgt gcc tat tcc acc aac ctg ttg agt gcg 1728

Asn Thr Thr Trp Tyr Trp Arg Ala Tyr Ser Thr Asn Leu Leu Ser Ala

565 570 575

gaa ttt tta aaa cag gtg caa agc cac ctt acc ccg gat ggt att gta 1776

Glu Phe Leu Lys Gln Val Gln Ser His Leu Thr Pro Asp Gly Ile Val

580 585 590

atg ttt aat acc acg cac agc ccg cat gct ttt gct acc gcc gta cac 1824

Met Phe Asn Thr Thr His Ser Pro His Ala Phe Ala Thr Ala Val His

595 600 605

agt att ccc tat gca tac cgc tat ggg cat atg gta gtc ggc tcg gca 1872

Ser Ile Pro Tyr Ala Tyr Arg Tyr Gly His Met Val Val Gly Ser Ala

610 615 620

acc ccg gta gtt ttc cct aat aaa gaa ctg ctc aag caa cgt ctc tcc 1920

Thr Pro Val Val Phe Pro Asn Lys Glu Leu Leu Lys Gln Arg Leu Ser

625 630 635 640

cgg ttg att tgg ccg gaa agc ggc agg cac gta ttt gac agc agc acc 1968

Arg Leu Ile Trp Pro Glu Ser Gly Arg His Val Phe Asp Ser Ser Thr

645 650 655

gtg gat gct gca gca caa aag gtt gtc tct cgt atg ctg att cag atg 2016

Val Asp Ala Ala Ala Gln Lys Val Val Ser Arg Met Leu Ile Gln Met

660 665 670

acg gaa cct tcg gct ggg gcg gaa gtc att acc gac gat aat atg att 2064

Thr Glu Pro Ser Ala Gly Ala Glu Val Ile Thr Asp Asp Asn Met Ile

675 680 685

gta gaa tac aaa tac ggc aga ggg att taa 2094

Val Glu Tyr Lys Tyr Gly Arg Gly Ile

690 695

<210> SEQ ID NO 52

<211> LENGTH: 697

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 52

Met Asn Ser Thr Ala Ser Lys Thr Leu Lys Gly Leu Ser Leu Val Phe

1 5 10 15

Phe Ala Ser Gly Phe Cys Ala Leu Ile Tyr Gln Val Ser Trp Gln Arg

20 25 30

Leu Leu Phe Ser His Ile Gly Ile Asp Leu Ser Ser Ile Thr Val Ile

35 40 45

Ile Ser Val Phe Met Val Gly Leu Gly Val Gly Ala Tyr Phe Gly Gly

50 55 60

Arg Ile Ala Asp Arg Phe Pro Ser Ser Ile Ile Pro Leu Phe Cys Ile

65 70 75 80

Ala Glu Val Ser Ile Gly Leu Phe Gly Leu Val Ser Arg Gly Leu Ile

85 90 95

Ser Gly Leu Gly His Leu Leu Val Glu Ala Asp Leu Pro Ile Ile Ala

100 105 110

Ala Ala Asn Phe Leu Leu Leu Leu Leu Pro Thr Phe Met Met Gly Ala

115 120 125

Thr Leu Pro Leu Leu Thr Cys Phe Phe Asn Arg Lys Ile His Asn Val

130 135 140

Gly Glu Ser Ile Gly Thr Leu Tyr Phe Phe Asn Thr Leu Gly Ala Ala

145 150 155 160

Leu Gly Ser Leu Ala Ala Ala Glu Phe Phe Tyr Val Phe Phe Thr Leu

165 170 175

Ser Gln Thr Ile Ala Leu Thr Ala Cys Phe Asn Leu Leu Ile Ala Ala

180 185 190

Ser Val Cys Cys Val Thr Glu Arg Met Asp Ile Val Asn Thr Lys Pro

195 200 205

Asn Thr Ser Leu Ile Tyr Met Leu Ser Phe Leu Ser Gly Leu Leu Ser

210 215 220

Leu Gly Ile Glu Val Leu Trp Val Arg Met Phe Ser Phe Ala Ala Gln

225 230 235 240

Ser Val Pro Gln Ala Phe Ser Phe Thr Leu Ala Tyr Phe Leu Thr Gly

245 250 255

Ile Ala Val Gly Ala Tyr Phe Gly Lys Arg Ile Cys Arg Ser Arg Phe

260 265 270

Val Asp Ile Pro Phe Ile Gly Gln Cys Phe Leu Trp Ala Gly Ile Ala

275 280 285

Asp Phe Leu Ile Leu Gly Ala Ala Trp Leu Leu Thr Gly Phe Ser Gly

290 295 300

Phe Val His His Ala Gly Ile Phe Ile Thr Leu Ser Ala Val Val Arg

305 310 315 320

Gly Leu Ile Phe Pro Leu Val His His Val Gly Thr Asp Gly Asn Lys

325 330 335

Ser Gly Arg Gln Val Ser Asn Val Tyr Phe Ala Asn Val Ala Gly Ser

340 345 350

Ala Leu Gly Pro Val Leu Ile Gly Phe Val Ile Leu Asp Phe Leu Ser

355 360 365

Thr Gln Gln Ile Tyr Leu Leu Ile Cys Leu Ile Ser Ala Ala Val Pro

370 375 380

Leu Phe Cys Thr Leu Phe Gln Lys Ser Leu Arg Leu Asn Ala Val Ser

385 390 395 400

Val Ala Val Ser Leu Met Phe Gly Ile Leu Met Phe Leu Leu Pro Asp

405 410 415

Ser Val Phe Gln Asn Ile Ala Asp Arg Pro Asp Arg Leu Ile Glu Asn

420 425 430

Lys His Gly Ile Val Ala Val Tyr His Arg Asp Gly Asp Lys Val Val

435 440 445

Tyr Gly Ala Asn Val Tyr Asp Gly Ala Tyr Asn Thr Asp Val Phe Asn

450 455 460

Ser Val Asn Gly Ile Glu Arg Ala Tyr Leu Leu Pro Ser Leu Lys Ser

465 470 475 480

Gly Ile Arg Arg Ile Phe Val Val Gly Leu Ser Thr Gly Ser Trp Ala

485 490 495

Arg Val Leu Ser Ala Ile Pro Glu Met Gln Ser Met Ile Val Ala Glu

500 505 510

Ile Asn Pro Ala Tyr Arg Ser Leu Ile Ala Asp Glu Pro Gln Ile Ala

515 520 525

Pro Leu Leu Gln Asp Lys Arg Val Glu Ile Val Leu Asp Asp Gly Arg

530 535 540

Lys Trp Leu Arg Arg His Pro Asp Glu Lys Phe Asp Leu Ile Leu Met

545 550 555 560

Asn Thr Thr Trp Tyr Trp Arg Ala Tyr Ser Thr Asn Leu Leu Ser Ala

565 570 575

Glu Phe Leu Lys Gln Val Gln Ser His Leu Thr Pro Asp Gly Ile Val

580 585 590

Met Phe Asn Thr Thr His Ser Pro His Ala Phe Ala Thr Ala Val His

595 600 605

Ser Ile Pro Tyr Ala Tyr Arg Tyr Gly His Met Val Val Gly Ser Ala

610 615 620

Thr Pro Val Val Phe Pro Asn Lys Glu Leu Leu Lys Gln Arg Leu Ser

625 630 635 640

Arg Leu Ile Trp Pro Glu Ser Gly Arg His Val Phe Asp Ser Ser Thr

645 650 655

Val Asp Ala Ala Ala Gln Lys Val Val Ser Arg Met Leu Ile Gln Met

660 665 670

Thr Glu Pro Ser Ala Gly Ala Glu Val Ile Thr Asp Asp Asn Met Ile

675 680 685

Val Glu Tyr Lys Tyr Gly Arg Gly Ile

690 695

<210> SEQ ID NO 53

<211> LENGTH: 1040

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 53

Cys Leu Gly Gly Gly Gly Gly Thr Ser Ala Pro Asp Phe Asn Ala Gly

1 5 10 15

Gly Thr Gly Ile Gly Ser Asn Ser Arg Ala Thr Thr Ala Lys Ser Ala

20 25 30

Ala Val Ser Tyr Ala Gly Ile Lys Asn Glu Met Cys Lys Asp Arg Ser

35 40 45

Met Leu Cys Ala Gly Arg Asp Asp Val Ala Val Thr Asp Arg Asp Ala

50 55 60

Lys Ile Asn Ala Pro Pro Pro Asn Leu His Thr Gly Asp Phe Thr Asn

65 70 75 80

Pro Asn Asp Ala Tyr Lys Asn Leu Ile Asn Leu Lys Pro Ala Ile Glu

85 90 95

Ala Gly Tyr Thr Gly Arg Gly Val Glu Val Gly Ile Val Asp Thr Gly

100 105 110

Glu Ser Val Gly Ser Ile Ser Phe Pro Glu Leu Tyr Gly Arg Lys Glu

115 120 125

His Gly Tyr Asn Glu Asn Tyr Lys Asn Tyr Thr Ala Tyr Met Arg Lys

130 135 140

Glu Ala Pro Glu Asp Gly Gly Gly Lys Asp Ile Lys Ala Ser Phe Asp

145 150 155 160

Asp Glu Ala Val Ile Glu Thr Glu Ala Lys Pro Thr Asp Ile Arg His

165 170 175

Val Lys Glu Ile Gly His Ile Asp Val Val Ser His Ile Ile Gly Gly

180 185 190

Arg Ser Val Asp Gly Arg Pro Ala Gly Gly Ile Ala Pro Asp Ala Thr

195 200 205

Leu His Ile Met Asn Thr His Asp Gly Thr Lys Asn Glu Ile Met Ser

210 215 220

Ala Ala Ile Arg Asn Ala Trp Val Lys Leu Gly Glu Arg Gly Val Arg

225 230 235 240

Ile Val Asn Asn Ser Phe Gly Thr Thr Ser Arg Ala Gly Thr Ala Asp

245 250 255

His Phe Gln Ile Ala Asn Ser Glu Glu Gln Tyr Arg Gln Ala Leu Leu

260 265 270

Ala Tyr Ser Gly Gly Asp Lys Thr Asp Glu Gly Ile Arg Leu Met Gln

275 280 285

Gln Ser Asp Tyr Gly Asn Leu Ser Tyr His Ile Arg Asn Lys Asn Met

290 295 300

Leu Phe Ile Phe Ser Ala Ser Asn Asp Ala Gln Ala Gln Pro Asn Thr

305 310 315 320

Leu Thr Leu Leu Pro Phe Tyr Glu Lys Asp Ala Gln Lys Gly Ile Ile

325 330 335

Thr Val Ala Gly Val Asp Arg Ser Gly Glu Lys Phe Asn Gly Ser Asn

340 345 350

His Cys Gly Ile Thr Ala Met Trp Cys Leu Ser Ala Pro Tyr Glu Ala

355 360 365

Ser Val Arg Phe Thr Arg Thr Asn Pro Ile Gln Ile Ala Gly Thr Ser

370 375 380

Phe Ser Ala Pro Ile Val Thr Gly Thr Ala Ala Leu Leu Leu Gln Lys

385 390 395 400

Tyr Pro Trp Met Ser Asn Asp Asn Leu Arg Thr Thr Leu Leu Thr Thr

405 410 415

Ala Gln Asp Ile Gly Ala Val Gly Val Asp Ser Lys Phe Gly Trp Gly

420 425 430

Leu Leu Asp Ala Gly Lys Ala Met Asn Gly Pro Ala Ser Phe Pro Phe

435 440 445

Gly Asp Phe Thr Ala Asp Thr Lys Gly Thr Ser Asp Ile Ala Tyr Ser

450 455 460

Phe Arg Asn Asp Ile Ser Gly Thr Gly Gly Leu Ile Lys Lys Gly Gly

465 470 475 480

Ser Gln Leu Gln Leu His Gly Asn Asn Thr Tyr Thr Gly Lys Thr Ile

485 490 495

Ile Glu Gly Gly Ser Leu Val Leu Tyr Gly Asn Asn Lys Ser Asp Met

500 505 510

Arg Val Glu Thr Lys Gly Ala Leu Ile Tyr Asn Gly Ala Ala Ser Gly

515 520 525

Gly Ser Leu Asn Ser Asp Gly Ile Val Tyr Leu Ala Asp Thr Asp Arg

530 535 540

Ser Gly Ala Asn Glu Thr Val His Ile Lys Gly Asp Leu Gln Leu Gly

545 550 555 560

Gly Glu Gly Thr Leu Tyr Thr Arg Leu Gly Lys Leu Leu Lys Val Asp

565 570 575

Gly Thr Ala Met Thr Gly Gly Lys Leu Tyr Met Ser Ala Arg Gly Lys

580 585 590

Gly Ala Gly Tyr Leu Asn Arg Thr Gly Gln Arg Val Pro Phe Leu Ser

595 600 605

Ala Ala Lys Ile Gly Arg Asp Tyr Ser Phe Phe Thr Asn Ile Glu Thr

610 615 620

Asp Gly Gly Leu Leu Ala Ser Leu Asp Ser Val Glu Lys Thr Ala Gly

625 630 635 640

Ser Glu Gly Asp Thr Leu Ser Tyr Tyr Val Arg Arg Gly Asn Ala Ala

645 650 655

Arg Thr Ala Ser Ala Ala Ala His Ser Ala Pro Ala Gly Leu Lys His

660 665 670

Ala Val Glu Gln Gly Gly Ser Asn Leu Glu Asn Leu Met Val Glu Leu

675 680 685

Asp Ala Ser Glu Ser Ser Ala Thr Pro Glu Thr Val Glu Thr Ala Ala

690 695 700

Ala Asp Arg Thr Asp Met Pro Gly Ile Arg Pro Tyr Gly Ala Thr Phe

705 710 715 720

Arg Ala Ala Ala Ala Val Gln His Ala Asn Ala Ala Asp Gly Val Arg

725 730 735

Ile Phe Asn Ser Leu Ala Ala Thr Val Tyr Ala Asp Ser Thr Ala Ala

740 745 750

His Ala Asp Met Gln Gly Arg Arg Leu Lys Ala Val Ser Asp Gly Leu

755 760 765

Asp His Asn Ala Thr Gly Leu Arg Val Ile Ala Gln Thr Gln Gln Asp

770 775 780

Gly Gly Thr Trp Glu Gln Gly Gly Val Glu Gly Lys Met Arg Gly Ser

785 790 795 800

Thr Gln Thr Val Gly Ile Ala Ala Lys Thr Gly Glu Asn Thr Thr Ala

805 810 815

Ala Ala Thr Leu Gly Met Gly His Ser Thr Trp Ser Glu Asn Ser Ala

820 825 830

Asn Ala Lys Thr Asp Ser Ile Ser Leu Phe Ala Gly Ile Arg His Asp

835 840 845

Ala Gly Asp Ile Gly Tyr Leu Lys Gly Leu Phe Ser Tyr Gly Arg Tyr

850 855 860

Lys Asn Ser Ile Ser Arg Ser Thr Gly Ala Asp Glu His Ala Glu Gly

865 870 875 880

Ser Val Asn Gly Thr Leu Met Gln Leu Gly Ala Leu Gly Gly Val Asn

885 890 895

Val Pro Phe Ala Ala Thr Gly Asp Leu Thr Val Glu Gly Gly Leu Arg

900 905 910

Tyr Asp Leu Leu Lys Gln Asp Ala Phe Ala Glu Lys Gly Ser Ala Leu

915 920 925

Gly Trp Ser Gly Asn Ser Leu Thr Glu Gly Thr Leu Val Gly Leu Ala

930 935 940

Gly Leu Lys Leu Ser Gln Pro Leu Ser Asp Lys Ala Val Leu Phe Ala

945 950 955 960

Thr Ala Gly Val Glu Arg Asp Leu Asn Gly Arg Asp Tyr Thr Val Thr

965 970 975

Gly Gly Phe Thr Gly Ala Thr Ala Ala Thr Gly Lys Thr Gly Ala Arg

980 985 990

Asn Met Pro His Thr Arg Leu Val Ala Gly Leu Gly Ala Asp Val Glu

995 1000 1005

Phe Gly Asn Gly Trp Asn Gly Leu Ala Arg Tyr Ser Tyr Ala Gly Ser

1010 1015 1020

Lys Gln Tyr Gly Asn His Ser Gly Arg Val Gly Val Gly Tyr Arg Phe

1025 1030 1035 1040

<210> SEQ ID NO 54

<211> LENGTH: 858

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(855)

<400> SEQUENCE: 54

atg tct gaa gaa aaa ttg aaa atg agt ttc gag cca acc gta atc gaa 48

Met Ser Glu Glu Lys Leu Lys Met Ser Phe Glu Pro Thr Val Ile Glu

1 5 10 15

cat ttg ggt gta aag atg tat tcg cac act gtt cct gcg att gcc gag 96

His Leu Gly Val Lys Met Tyr Ser His Thr Val Pro Ala Ile Ala Glu

20 25 30

ttg ata gcg aat gcc tac gat gca tgt gct acg gaa gtg gaa gtt agg 144

Leu Ile Ala Asn Ala Tyr Asp Ala Cys Ala Thr Glu Val Glu Val Arg

35 40 45

tta ttc gat aaa ccg gag cat aaa atc gtt att aaa gat aat ggc ata 192

Leu Phe Asp Lys Pro Glu His Lys Ile Val Ile Lys Asp Asn Gly Ile

50 55 60

gga atg agc ttc gat gaa atc aat gat ttt tat ttg aga atc ggt cgg 240

Gly Met Ser Phe Asp Glu Ile Asn Asp Phe Tyr Leu Arg Ile Gly Arg

65 70 75 80

aac aga agg gaa gaa aaa caa gcc tcc ccg tgc gga aga att cca acg 288

Asn Arg Arg Glu Glu Lys Gln Ala Ser Pro Cys Gly Arg Ile Pro Thr

85 90 95

ggt aaa aaa ggt ctt ggt aaa ttg gca tta ttc agg ctt ggc aac aaa 336

Gly Lys Lys Gly Leu Gly Lys Leu Ala Leu Phe Arg Leu Gly Asn Lys

100 105 110

atc gaa atc tct act atc caa gga aac gaa cgg gtt act ttt act ttg 384

Ile Glu Ile Ser Thr Ile Gln Gly Asn Glu Arg Val Thr Phe Thr Leu

115 120 125

gat tat gca gag att aaa aaa agt gag cgt att tat caa ccg gag ttt 432

Asp Tyr Ala Glu Ile Lys Lys Ser Glu Arg Ile Tyr Gln Pro Glu Phe

130 135 140

cag aaa gag tct gtt aaa ccc aat acc gaa aac gga aca act ata act 480

Gln Lys Glu Ser Val Lys Pro Asn Thr Glu Asn Gly Thr Thr Ile Thr

145 150 155 160

tta acc gag ctg acg aaa aaa caa gga tac ccg tta gat aat tat gtg 528

Leu Thr Glu Leu Thr Lys Lys Gln Gly Tyr Pro Leu Asp Asn Tyr Val

165 170 175

ggg cat ctt tcc cgt tta ttt gat ttt ccg gct cag gat ttt aaa atc 576

Gly His Leu Ser Arg Leu Phe Asp Phe Pro Ala Gln Asp Phe Lys Ile

180 185 190

aaa gta agc ttg aac ggc tcg gaa cca aga atc att gac gga aac cta 624

Lys Val Ser Leu Asn Gly Ser Glu Pro Arg Ile Ile Asp Gly Asn Leu

195 200 205

aaa tat aat ctt gtt acc cca caa ttc gaa tgg gaa tac cag gat cta 672

Lys Tyr Asn Leu Val Thr Pro Gln Phe Glu Trp Glu Tyr Gln Asp Leu

210 215 220

gca acc aat att tca tcg tta tct tca aaa ttc gaa cag tat gaa tac 720

Ala Thr Asn Ile Ser Ser Leu Ser Ser Lys Phe Glu Gln Tyr Glu Tyr

225 230 235 240

agc gga tta ata caa ggt aag ttc att aca acg gaa aaa cct tta aag 768

Ser Gly Leu Ile Gln Gly Lys Phe Ile Thr Thr Glu Lys Pro Leu Lys

245 250 255

aat aat atg aaa ggt att acc ttg ttt gcc aac ggc aga atg gta aat 816

Asn Asn Met Lys Gly Ile Thr Leu Phe Ala Asn Gly Arg Met Val Asn

260 265 270

atg ccc gag ttt ttc act gat agc gaa tcc agc cat ttc taa 858

Met Pro Glu Phe Phe Thr Asp Ser Glu Ser Ser His Phe

275 280 285

<210> SEQ ID NO 55

<211> LENGTH: 285

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 55

Met Ser Glu Glu Lys Leu Lys Met Ser Phe Glu Pro Thr Val Ile Glu

1 5 10 15

His Leu Gly Val Lys Met Tyr Ser His Thr Val Pro Ala Ile Ala Glu

20 25 30

Leu Ile Ala Asn Ala Tyr Asp Ala Cys Ala Thr Glu Val Glu Val Arg

35 40 45

Leu Phe Asp Lys Pro Glu His Lys Ile Val Ile Lys Asp Asn Gly Ile

50 55 60

Gly Met Ser Phe Asp Glu Ile Asn Asp Phe Tyr Leu Arg Ile Gly Arg

65 70 75 80

Asn Arg Arg Glu Glu Lys Gln Ala Ser Pro Cys Gly Arg Ile Pro Thr

85 90 95

Gly Lys Lys Gly Leu Gly Lys Leu Ala Leu Phe Arg Leu Gly Asn Lys

100 105 110

Ile Glu Ile Ser Thr Ile Gln Gly Asn Glu Arg Val Thr Phe Thr Leu

115 120 125

Asp Tyr Ala Glu Ile Lys Lys Ser Glu Arg Ile Tyr Gln Pro Glu Phe

130 135 140

Gln Lys Glu Ser Val Lys Pro Asn Thr Glu Asn Gly Thr Thr Ile Thr

145 150 155 160

Leu Thr Glu Leu Thr Lys Lys Gln Gly Tyr Pro Leu Asp Asn Tyr Val

165 170 175

Gly His Leu Ser Arg Leu Phe Asp Phe Pro Ala Gln Asp Phe Lys Ile

180 185 190

Lys Val Ser Leu Asn Gly Ser Glu Pro Arg Ile Ile Asp Gly Asn Leu

195 200 205

Lys Tyr Asn Leu Val Thr Pro Gln Phe Glu Trp Glu Tyr Gln Asp Leu

210 215 220

Ala Thr Asn Ile Ser Ser Leu Ser Ser Lys Phe Glu Gln Tyr Glu Tyr

225 230 235 240

Ser Gly Leu Ile Gln Gly Lys Phe Ile Thr Thr Glu Lys Pro Leu Lys

245 250 255

Asn Asn Met Lys Gly Ile Thr Leu Phe Ala Asn Gly Arg Met Val Asn

260 265 270

Met Pro Glu Phe Phe Thr Asp Ser Glu Ser Ser His Phe

275 280 285

<210> SEQ ID NO 56

<211> LENGTH: 1575

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1572)

<400> SEQUENCE: 56

atg aaa aaa tcc ctt ttc gtt ctc ttt ctg tat tca tcc cta ctt acc 48

Met Lys Lys Ser Leu Phe Val Leu Phe Leu Tyr Ser Ser Leu Leu Thr

1 5 10 15

gcc agc gaa atc gcc tat cgc ttt gta ttc gga att gaa acc tta ccg 96

Ala Ser Glu Ile Ala Tyr Arg Phe Val Phe Gly Ile Glu Thr Leu Pro

20 25 30

gct gca aaa atg gcg gaa acg ttt gcg ctg aca ttt atg att gct gcg 144

Ala Ala Lys Met Ala Glu Thr Phe Ala Leu Thr Phe Met Ile Ala Ala

35 40 45

ctg tat ctg ttt gcg cgt tat aag gct tcg cgg ctg ctg att gcg gtg 192

Leu Tyr Leu Phe Ala Arg Tyr Lys Ala Ser Arg Leu Leu Ile Ala Val

50 55 60

ttt ttc gcg ttc agc atg att gcc aac aat gtg cat tac gcg gtt tat 240

Phe Phe Ala Phe Ser Met Ile Ala Asn Asn Val His Tyr Ala Val Tyr

65 70 75 80

caa agc tgg atg acg ggt att aac tat tgg ctg atg ctg aaa gag gtt 288

Gln Ser Trp Met Thr Gly Ile Asn Tyr Trp Leu Met Leu Lys Glu Val

85 90 95

acc gaa gtc ggc agc gcg ggc gcg tcg atg ttg gat aag ttg tgg ctg 336

Thr Glu Val Gly Ser Ala Gly Ala Ser Met Leu Asp Lys Leu Trp Leu

100 105 110

cct gct ttg tgg ggc gtg gcg gaa gtc atg ttg ttt tgc agc ctt gcc 384

Pro Ala Leu Trp Gly Val Ala Glu Val Met Leu Phe Cys Ser Leu Ala

115 120 125

aag ttc cgc cgt aag acg cat ttt tct gcc gat ata ctg ttt gcc ttc 432

Lys Phe Arg Arg Lys Thr His Phe Ser Ala Asp Ile Leu Phe Ala Phe

130 135 140

cta atg ctg atg att ttc gtg cgt tcg ttc gac acg aaa caa gag cac 480

Leu Met Leu Met Ile Phe Val Arg Ser Phe Asp Thr Lys Gln Glu His

145 150 155 160

ggt att tcg ccc aaa ccg aca tac agc cgc atc aaa gcc aat tat ttc 528

Gly Ile Ser Pro Lys Pro Thr Tyr Ser Arg Ile Lys Ala Asn Tyr Phe

165 170 175

agc ttc ggt tat ttt gtc ggg cgc gtg ttg ccg tat cag ttg ttt gat 576

Ser Phe Gly Tyr Phe Val Gly Arg Val Leu Pro Tyr Gln Leu Phe Asp

180 185 190

tta agc aag atc cct gtg ttc aaa cag cct gct cca agc aaa atc ggg 624

Leu Ser Lys Ile Pro Val Phe Lys Gln Pro Ala Pro Ser Lys Ile Gly

195 200 205

caa ggc agt att caa aat atc gtc ctg att atg ggc gaa agc gaa agc 672

Gln Gly Ser Ile Gln Asn Ile Val Leu Ile Met Gly Glu Ser Glu Ser

210 215 220

gcg gcg cat ttg aaa ttg ttt ggt tac ggg cgc gaa act tcg ccg ttt 720

Ala Ala His Leu Lys Leu Phe Gly Tyr Gly Arg Glu Thr Ser Pro Phe

225 230 235 240

tta acc cgg ctg tcg caa gcc gat ttt aag ccg att gtg aaa caa agt 768

Leu Thr Arg Leu Ser Gln Ala Asp Phe Lys Pro Ile Val Lys Gln Ser

245 250 255

tat tcc gca ggc ttt atg acg gca gta tcc ctg ccc agt ttc ttt aac 816

Tyr Ser Ala Gly Phe Met Thr Ala Val Ser Leu Pro Ser Phe Phe Asn

260 265 270

gtc ata ccg cac gcc aac ggc ttg gaa caa atc agc ggc ggc gat acc 864

Val Ile Pro His Ala Asn Gly Leu Glu Gln Ile Ser Gly Gly Asp Thr

275 280 285

aat atg ttc cgc ctc gcc aaa gag cag ggc tat gaa acg tat ttt tac 912

Asn Met Phe Arg Leu Ala Lys Glu Gln Gly Tyr Glu Thr Tyr Phe Tyr

290 295 300

agt gcc cag gct gaa aac caa atg gca att ttg aac tta atc ggt aag 960

Ser Ala Gln Ala Glu Asn Gln Met Ala Ile Leu Asn Leu Ile Gly Lys

305 310 315 320

aaa tgg ata gac cat ctg att cag ccg acg caa ctt ggc tac ggc aac 1008

Lys Trp Ile Asp His Leu Ile Gln Pro Thr Gln Leu Gly Tyr Gly Asn

325 330 335

ggc gac aat atg ccc gat gag aag ctg ctg ccg ttg ttc gac aaa atc 1056

Gly Asp Asn Met Pro Asp Glu Lys Leu Leu Pro Leu Phe Asp Lys Ile

340 345 350

aat ttg cag cag ggc agg cat ttt atc gtg ttg cac caa cgc ggt tcg 1104

Asn Leu Gln Gln Gly Arg His Phe Ile Val Leu His Gln Arg Gly Ser

355 360 365

cac gcc cca tac ggc gca ttg ttg cag cct caa gat aaa gta ttc ggc 1152

His Ala Pro Tyr Gly Ala Leu Leu Gln Pro Gln Asp Lys Val Phe Gly

370 375 380

gaa gcc gat att gtg gat aag tac gac aac acc atc cac aaa acc gac 1200

Glu Ala Asp Ile Val Asp Lys Tyr Asp Asn Thr Ile His Lys Thr Asp

385 390 395 400

caa atg att caa acc gta ttc gag cag ctg caa aag cag cct gac ggc 1248

Gln Met Ile Gln Thr Val Phe Glu Gln Leu Gln Lys Gln Pro Asp Gly

405 410 415

aac tgg ctg ttt gcc tat acc tcc gat cat ggc cag tat gtg cgc caa 1296

Asn Trp Leu Phe Ala Tyr Thr Ser Asp His Gly Gln Tyr Val Arg Gln

420 425 430

gat atc tac aat caa ggc acg gtg cag ccc gac agc tat att gtg cct 1344

Asp Ile Tyr Asn Gln Gly Thr Val Gln Pro Asp Ser Tyr Ile Val Pro

435 440 445

ctg gtt ttg tac agc ccg gat aag gcc gtg caa cag gct gcc aac cag 1392

Leu Val Leu Tyr Ser Pro Asp Lys Ala Val Gln Gln Ala Ala Asn Gln

450 455 460

gct ttt gcg cct tgc gag att gcc ttc cat cag cag ctt tca acg ttc 1440

Ala Phe Ala Pro Cys Glu Ile Ala Phe His Gln Gln Leu Ser Thr Phe

465 470 475 480

ctg att cac acg ttg ggc tac gat atg ccg gtt tca ggt tgt cgc gaa 1488

Leu Ile His Thr Leu Gly Tyr Asp Met Pro Val Ser Gly Cys Arg Glu

485 490 495

ggc tcg gta aca ggc aac ctg att acg ggc gat gca ggc agc ttg aac 1536

Gly Ser Val Thr Gly Asn Leu Ile Thr Gly Asp Ala Gly Ser Leu Asn

500 505 510

att cgc aac ggc aag gcg gaa tat gtt tat ccg caa taa 1575

Ile Arg Asn Gly Lys Ala Glu Tyr Val Tyr Pro Gln

515 520

<210> SEQ ID NO 57

<211> LENGTH: 524

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 57

Met Lys Lys Ser Leu Phe Val Leu Phe Leu Tyr Ser Ser Leu Leu Thr

1 5 10 15

Ala Ser Glu Ile Ala Tyr Arg Phe Val Phe Gly Ile Glu Thr Leu Pro

20 25 30

Ala Ala Lys Met Ala Glu Thr Phe Ala Leu Thr Phe Met Ile Ala Ala

35 40 45

Leu Tyr Leu Phe Ala Arg Tyr Lys Ala Ser Arg Leu Leu Ile Ala Val

50 55 60

Phe Phe Ala Phe Ser Met Ile Ala Asn Asn Val His Tyr Ala Val Tyr

65 70 75 80

Gln Ser Trp Met Thr Gly Ile Asn Tyr Trp Leu Met Leu Lys Glu Val

85 90 95

Thr Glu Val Gly Ser Ala Gly Ala Ser Met Leu Asp Lys Leu Trp Leu

100 105 110

Pro Ala Leu Trp Gly Val Ala Glu Val Met Leu Phe Cys Ser Leu Ala

115 120 125

Lys Phe Arg Arg Lys Thr His Phe Ser Ala Asp Ile Leu Phe Ala Phe

130 135 140

Leu Met Leu Met Ile Phe Val Arg Ser Phe Asp Thr Lys Gln Glu His

145 150 155 160

Gly Ile Ser Pro Lys Pro Thr Tyr Ser Arg Ile Lys Ala Asn Tyr Phe

165 170 175

Ser Phe Gly Tyr Phe Val Gly Arg Val Leu Pro Tyr Gln Leu Phe Asp

180 185 190

Leu Ser Lys Ile Pro Val Phe Lys Gln Pro Ala Pro Ser Lys Ile Gly

195 200 205

Gln Gly Ser Ile Gln Asn Ile Val Leu Ile Met Gly Glu Ser Glu Ser

210 215 220

Ala Ala His Leu Lys Leu Phe Gly Tyr Gly Arg Glu Thr Ser Pro Phe

225 230 235 240

Leu Thr Arg Leu Ser Gln Ala Asp Phe Lys Pro Ile Val Lys Gln Ser

245 250 255

Tyr Ser Ala Gly Phe Met Thr Ala Val Ser Leu Pro Ser Phe Phe Asn

260 265 270

Val Ile Pro His Ala Asn Gly Leu Glu Gln Ile Ser Gly Gly Asp Thr

275 280 285

Asn Met Phe Arg Leu Ala Lys Glu Gln Gly Tyr Glu Thr Tyr Phe Tyr

290 295 300

Ser Ala Gln Ala Glu Asn Gln Met Ala Ile Leu Asn Leu Ile Gly Lys

305 310 315 320

Lys Trp Ile Asp His Leu Ile Gln Pro Thr Gln Leu Gly Tyr Gly Asn

325 330 335

Gly Asp Asn Met Pro Asp Glu Lys Leu Leu Pro Leu Phe Asp Lys Ile

340 345 350

Asn Leu Gln Gln Gly Arg His Phe Ile Val Leu His Gln Arg Gly Ser

355 360 365

His Ala Pro Tyr Gly Ala Leu Leu Gln Pro Gln Asp Lys Val Phe Gly

370 375 380

Glu Ala Asp Ile Val Asp Lys Tyr Asp Asn Thr Ile His Lys Thr Asp

385 390 395 400

Gln Met Ile Gln Thr Val Phe Glu Gln Leu Gln Lys Gln Pro Asp Gly

405 410 415

Asn Trp Leu Phe Ala Tyr Thr Ser Asp His Gly Gln Tyr Val Arg Gln

420 425 430

Asp Ile Tyr Asn Gln Gly Thr Val Gln Pro Asp Ser Tyr Ile Val Pro

435 440 445

Leu Val Leu Tyr Ser Pro Asp Lys Ala Val Gln Gln Ala Ala Asn Gln

450 455 460

Ala Phe Ala Pro Cys Glu Ile Ala Phe His Gln Gln Leu Ser Thr Phe

465 470 475 480

Leu Ile His Thr Leu Gly Tyr Asp Met Pro Val Ser Gly Cys Arg Glu

485 490 495

Gly Ser Val Thr Gly Asn Leu Ile Thr Gly Asp Ala Gly Ser Leu Asn

500 505 510

Ile Arg Asn Gly Lys Ala Glu Tyr Val Tyr Pro Gln

515 520

<210> SEQ ID NO 58

<211> LENGTH: 1314

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1311)

<400> SEQUENCE: 58

atg ctg acg ttt atc gga ttg ctg att atc ggg gtc atc gta tgg ctg 48

Met Leu Thr Phe Ile Gly Leu Leu Ile Ile Gly Val Ile Val Trp Leu

1 5 10 15

ttg ctg acg gaa aaa gtg tcg ccc atc atc gca tta atc ttg gtg ccg 96

Leu Leu Thr Glu Lys Val Ser Pro Ile Ile Ala Leu Ile Leu Val Pro

20 25 30

ctg att ggg gcg ttg ctg gcg ggg ttt gat gta tcc caa tta aaa gaa 144

Leu Ile Gly Ala Leu Leu Ala Gly Phe Asp Val Ser Gln Leu Lys Glu

35 40 45

ttt tat tcg ggc ggc acg aaa tcg gtg acg cag att gtg att atg ttt 192

Phe Tyr Ser Gly Gly Thr Lys Ser Val Thr Gln Ile Val Ile Met Phe

50 55 60

atg ttt tcc att ttg ttt ttt gga atc atg aac gat gtg ggg ctg ttc 240

Met Phe Ser Ile Leu Phe Phe Gly Ile Met Asn Asp Val Gly Leu Phe

65 70 75 80

cgt ccg atg ata ggc ggt ttg att aag ctg act cgg ggt aat atc gtg 288

Arg Pro Met Ile Gly Gly Leu Ile Lys Leu Thr Arg Gly Asn Ile Val

85 90 95

gca gtg agt gtg ggg acg gtc ttg gtg tcg gtg gtg gca cag ttg gac 336

Ala Val Ser Val Gly Thr Val Leu Val Ser Val Val Ala Gln Leu Asp

100 105 110

ggg gcg ggc gcg acg acg ttt tta tcg gtc gtc ccc gcc ctt ttg ccg 384

Gly Ala Gly Ala Thr Thr Phe Leu Ser Val Val Pro Ala Leu Leu Pro

115 120 125

ctt tac aag cgt ctg cat atg aat cct tac ctg ctg ttt ttg ctg ctg 432

Leu Tyr Lys Arg Leu His Met Asn Pro Tyr Leu Leu Phe Leu Leu Leu

130 135 140

act tcc agc gcg ggg cta atc aac ctt ttg ccg cgg ggc ggg ccg atc 480

Thr Ser Ser Ala Gly Leu Ile Asn Leu Leu Pro Arg Gly Gly Pro Ile

145 150 155 160

ggg cgg gtt gca agc gtg ttg ggc gca gat gtg ggc gaa ttg tat aaa 528

Gly Arg Val Ala Ser Val Leu Gly Ala Asp Val Gly Glu Leu Tyr Lys

165 170 175

cct ttg ttg acg gtg caa att atc ggt gtg gtg ttt atc ctt gtg ctg 576

Pro Leu Leu Thr Val Gln Ile Ile Gly Val Val Phe Ile Leu Val Leu

180 185 190

tcc ctg ttt ttg ggt gtg cgt gaa aaa agg cgg att gtc cgg gag ttg 624

Ser Leu Phe Leu Gly Val Arg Glu Lys Arg Arg Ile Val Arg Glu Leu

195 200 205

ggc gcg ttg ccc gcc gtg gcg gat ttg ata aag ccg gcg cct ttg tcg 672

Gly Ala Leu Pro Ala Val Ala Asp Leu Ile Lys Pro Ala Pro Leu Ser

210 215 220

gaa gaa gaa caa aaa ttg gcg cgt ccg aaa ctg ttt tgg tgg aat gtc 720

Glu Glu Glu Gln Lys Leu Ala Arg Pro Lys Leu Phe Trp Trp Asn Val

225 230 235 240

ctg ctg ttt ttg gcg gcg atg agc ctg ctt ttt tcg ggc atc ttc ccg 768

Leu Leu Phe Leu Ala Ala Met Ser Leu Leu Phe Ser Gly Ile Phe Pro

245 250 255

ccg ggt tat gta ttt atg ctg gct gca acg gcg gcg ttg ctt ttg aat 816

Pro Gly Tyr Val Phe Met Leu Ala Ala Thr Ala Ala Leu Leu Leu Asn

260 265 270

tac cgc agc ccg cag gaa cag atg gag cgg att tat gcc cac gcc ggc 864

Tyr Arg Ser Pro Gln Glu Gln Met Glu Arg Ile Tyr Ala His Ala Gly

275 280 285

ggc gcg gtg atg atg gcg tcc att att ttg gcg gca ggt acg ttt ttg 912

Gly Ala Val Met Met Ala Ser Ile Ile Leu Ala Ala Gly Thr Phe Leu

290 295 300

ggg att ttg aag ggc gcg ggg atg ttg gac gcg att tcc aaa gac ctt 960

Gly Ile Leu Lys Gly Ala Gly Met Leu Asp Ala Ile Ser Lys Asp Leu

305 310 315 320

gtg cat atc ctg ccg gac gcg ttg ctg cct tat ctg cat att gcc atc 1008

Val His Ile Leu Pro Asp Ala Leu Leu Pro Tyr Leu His Ile Ala Ile

325 330 335

ggt gtg ttg ggt att ccg ctt gag ttg gtt ttg agt acg gac gct tat 1056

Gly Val Leu Gly Ile Pro Leu Glu Leu Val Leu Ser Thr Asp Ala Tyr

340 345 350

tat ttc gga ctg ttt ccg att gtg gaa cag att acc tcg cag gcg ggc 1104

Tyr Phe Gly Leu Phe Pro Ile Val Glu Gln Ile Thr Ser Gln Ala Gly

355 360 365

gtt gca ccc gaa gcg gca ggc tat gcg atg ttg atc ggc agt atc gtc 1152

Val Ala Pro Glu Ala Ala Gly Tyr Ala Met Leu Ile Gly Ser Ile Val

370 375 380

ggt act ttt gtt acg ccg ctt tcg ccg gct ttg tgg atg ggt ttg ggt 1200

Gly Thr Phe Val Thr Pro Leu Ser Pro Ala Leu Trp Met Gly Leu Gly

385 390 395 400

ttg gcg aaa ttg tcg atg ggc aaa cac atc cgt tat tcg ttt ttc tgg 1248

Leu Ala Lys Leu Ser Met Gly Lys His Ile Arg Tyr Ser Phe Phe Trp

405 410 415

gcg tgg ggt ttg tcg ctg gcg ata ttg gtc agt tcg ata gcg gca gga 1296

Ala Trp Gly Leu Ser Leu Ala Ile Leu Val Ser Ser Ile Ala Ala Gly

420 425 430

atc gtg cct ctg ccg taa 1314

Ile Val Pro Leu Pro

435

<210> SEQ ID NO 59

<211> LENGTH: 437

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 59

Met Leu Thr Phe Ile Gly Leu Leu Ile Ile Gly Val Ile Val Trp Leu

1 5 10 15

Leu Leu Thr Glu Lys Val Ser Pro Ile Ile Ala Leu Ile Leu Val Pro

20 25 30

Leu Ile Gly Ala Leu Leu Ala Gly Phe Asp Val Ser Gln Leu Lys Glu

35 40 45

Phe Tyr Ser Gly Gly Thr Lys Ser Val Thr Gln Ile Val Ile Met Phe

50 55 60

Met Phe Ser Ile Leu Phe Phe Gly Ile Met Asn Asp Val Gly Leu Phe

65 70 75 80

Arg Pro Met Ile Gly Gly Leu Ile Lys Leu Thr Arg Gly Asn Ile Val

85 90 95

Ala Val Ser Val Gly Thr Val Leu Val Ser Val Val Ala Gln Leu Asp

100 105 110

Gly Ala Gly Ala Thr Thr Phe Leu Ser Val Val Pro Ala Leu Leu Pro

115 120 125

Leu Tyr Lys Arg Leu His Met Asn Pro Tyr Leu Leu Phe Leu Leu Leu

130 135 140

Thr Ser Ser Ala Gly Leu Ile Asn Leu Leu Pro Arg Gly Gly Pro Ile

145 150 155 160

Gly Arg Val Ala Ser Val Leu Gly Ala Asp Val Gly Glu Leu Tyr Lys

165 170 175

Pro Leu Leu Thr Val Gln Ile Ile Gly Val Val Phe Ile Leu Val Leu

180 185 190

Ser Leu Phe Leu Gly Val Arg Glu Lys Arg Arg Ile Val Arg Glu Leu

195 200 205

Gly Ala Leu Pro Ala Val Ala Asp Leu Ile Lys Pro Ala Pro Leu Ser

210 215 220

Glu Glu Glu Gln Lys Leu Ala Arg Pro Lys Leu Phe Trp Trp Asn Val

225 230 235 240

Leu Leu Phe Leu Ala Ala Met Ser Leu Leu Phe Ser Gly Ile Phe Pro

245 250 255

Pro Gly Tyr Val Phe Met Leu Ala Ala Thr Ala Ala Leu Leu Leu Asn

260 265 270

Tyr Arg Ser Pro Gln Glu Gln Met Glu Arg Ile Tyr Ala His Ala Gly

275 280 285

Gly Ala Val Met Met Ala Ser Ile Ile Leu Ala Ala Gly Thr Phe Leu

290 295 300

Gly Ile Leu Lys Gly Ala Gly Met Leu Asp Ala Ile Ser Lys Asp Leu

305 310 315 320

Val His Ile Leu Pro Asp Ala Leu Leu Pro Tyr Leu His Ile Ala Ile

325 330 335

Gly Val Leu Gly Ile Pro Leu Glu Leu Val Leu Ser Thr Asp Ala Tyr

340 345 350

Tyr Phe Gly Leu Phe Pro Ile Val Glu Gln Ile Thr Ser Gln Ala Gly

355 360 365

Val Ala Pro Glu Ala Ala Gly Tyr Ala Met Leu Ile Gly Ser Ile Val

370 375 380

Gly Thr Phe Val Thr Pro Leu Ser Pro Ala Leu Trp Met Gly Leu Gly

385 390 395 400

Leu Ala Lys Leu Ser Met Gly Lys His Ile Arg Tyr Ser Phe Phe Trp

405 410 415

Ala Trp Gly Leu Ser Leu Ala Ile Leu Val Ser Ser Ile Ala Ala Gly

420 425 430

Ile Val Pro Leu Pro

435

<210> SEQ ID NO 60

<211> LENGTH: 1155

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1152)

<400> SEQUENCE: 60

atg ggc atc cat ctc gac ttc ggc att agt cct aaa acg ttc cga cag 48

Met Gly Ile His Leu Asp Phe Gly Ile Ser Pro Lys Thr Phe Arg Gln

1 5 10 15

act tat ctg tat caa aag ccc aag ctc ttt aaa gga gcg gtt cgg aat 96

Thr Tyr Leu Tyr Gln Lys Pro Lys Leu Phe Lys Gly Ala Val Arg Asn

20 25 30

ctc gaa gcc gca tct tgt aaa tat atc aac gag ata tac caa cga gca 144

Leu Glu Ala Ala Ser Cys Lys Tyr Ile Asn Glu Ile Tyr Gln Arg Ala

35 40 45

gac cca acc gca ccg ctg ttt cat ctg cgt aaa aaa ggc gca atc gtt 192

Asp Pro Thr Ala Pro Leu Phe His Leu Arg Lys Lys Gly Ala Ile Val

50 55 60

cct aaa gaa gaa tac gtc gaa agt ttc gac gat ttg ggc aaa act cgc 240

Pro Lys Glu Glu Tyr Val Glu Ser Phe Asp Asp Leu Gly Lys Thr Arg

65 70 75 80

tac cgt ttt att aaa tcc gtt atc tac gaa cat atg aag aat ggt gcg 288

Tyr Arg Phe Ile Lys Ser Val Ile Tyr Glu His Met Lys Asn Gly Ala

85 90 95

tcg tta gtc tat aac cat att aac aac gag ccg ttt tca gac cat atc 336

Ser Leu Val Tyr Asn His Ile Asn Asn Glu Pro Phe Ser Asp His Ile

100 105 110

gcc cgt caa gtc gcc cgc ttt gcc ggc gca cat act att gtt agt gga 384

Ala Arg Gln Val Ala Arg Phe Ala Gly Ala His Thr Ile Val Ser Gly

115 120 125

tat ctt gct ttt ggc agc gac gaa tct tat aaa aac cat tgg gat acc 432

Tyr Leu Ala Phe Gly Ser Asp Glu Ser Tyr Lys Asn His Trp Asp Thr

130 135 140

cgc gat gtg tat gcc atc cag ctt ttc ggc aag aaa cgt tgg caa ctt 480

Arg Asp Val Tyr Ala Ile Gln Leu Phe Gly Lys Lys Arg Trp Gln Leu

145 150 155 160

act gcc cct gat ttc cct atg cca ttg tat atg caa cag act aaa gat 528

Thr Ala Pro Asp Phe Pro Met Pro Leu Tyr Met Gln Gln Thr Lys Asp

165 170 175

act gat att tcc att cct gaa cat atc gat atg gat att atc ctt gaa 576

Thr Asp Ile Ser Ile Pro Glu His Ile Asp Met Asp Ile Ile Leu Glu

180 185 190

gca ggt gat gtc ctc tac atc cca cgc ggt tgg tgg cac aga cct atc 624

Ala Gly Asp Val Leu Tyr Ile Pro Arg Gly Trp Trp His Arg Pro Ile

195 200 205

ccg ctc ggc tgt gaa acc ttc cac ttc gct gtc ggt acc ttc cca cca 672

Pro Leu Gly Cys Glu Thr Phe His Phe Ala Val Gly Thr Phe Pro Pro

210 215 220

aac ggc tat aat tac ctc gag tgg cta atg aag aaa ttt ccc acc ata 720

Asn Gly Tyr Asn Tyr Leu Glu Trp Leu Met Lys Lys Phe Pro Thr Ile

225 230 235 240

gaa agt ctg cgc cac agt ttc tca gac tgg gag caa gat agg acg cgt 768

Glu Ser Leu Arg His Ser Phe Ser Asp Trp Glu Gln Asp Arg Thr Arg

245 250 255

atc aac gat act gcc gca caa att gct gcc atg att gcc gac ccc gtc 816

Ile Asn Asp Thr Ala Ala Gln Ile Ala Ala Met Ile Ala Asp Pro Val

260 265 270

aat tat gaa gcc ttc agt gaa gac ttt ctc ggc aaa gaa cgt acc gat 864

Asn Tyr Glu Ala Phe Ser Glu Asp Phe Leu Gly Lys Glu Arg Thr Asp

275 280 285

acc gct ttt cat ctc gaa cag ttc gcg aat ccc aac gct act ccg ctt 912

Thr Ala Phe His Leu Glu Gln Phe Ala Asn Pro Asn Ala Thr Pro Leu

290 295 300

tca gac gac gtc agg ttg aga tta aat gcc aat aat ttg gat acg ttg 960

Ser Asp Asp Val Arg Leu Arg Leu Asn Ala Asn Asn Leu Asp Thr Leu

305 310 315 320

gaa aag gga tat ttg att ggg aat ggg atg aag ata agc gta gat gag 1008

Glu Lys Gly Tyr Leu Ile Gly Asn Gly Met Lys Ile Ser Val Asp Glu

325 330 335

ttg ggg aaa aaa gtg tta gaa cac atc ggt aag aat gaa ccg tta ttg 1056

Leu Gly Lys Lys Val Leu Glu His Ile Gly Lys Asn Glu Pro Leu Leu

340 345 350

ttg aaa aat cta ctg gtt aac ttc aat cag gca aaa cat gaa gaa gtt 1104

Leu Lys Asn Leu Leu Val Asn Phe Asn Gln Ala Lys His Glu Glu Val

355 360 365

agg aag ttg atc tat cag ttg ata gag tta gat ttt ctg gaa att ttg 1152

Arg Lys Leu Ile Tyr Gln Leu Ile Glu Leu Asp Phe Leu Glu Ile Leu

370 375 380

tga 1155

<210> SEQ ID NO 61

<211> LENGTH: 384

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 61

Met Gly Ile His Leu Asp Phe Gly Ile Ser Pro Lys Thr Phe Arg Gln

1 5 10 15

Thr Tyr Leu Tyr Gln Lys Pro Lys Leu Phe Lys Gly Ala Val Arg Asn

20 25 30

Leu Glu Ala Ala Ser Cys Lys Tyr Ile Asn Glu Ile Tyr Gln Arg Ala

35 40 45

Asp Pro Thr Ala Pro Leu Phe His Leu Arg Lys Lys Gly Ala Ile Val

50 55 60

Pro Lys Glu Glu Tyr Val Glu Ser Phe Asp Asp Leu Gly Lys Thr Arg

65 70 75 80

Tyr Arg Phe Ile Lys Ser Val Ile Tyr Glu His Met Lys Asn Gly Ala

85 90 95

Ser Leu Val Tyr Asn His Ile Asn Asn Glu Pro Phe Ser Asp His Ile

100 105 110

Ala Arg Gln Val Ala Arg Phe Ala Gly Ala His Thr Ile Val Ser Gly

115 120 125

Tyr Leu Ala Phe Gly Ser Asp Glu Ser Tyr Lys Asn His Trp Asp Thr

130 135 140

Arg Asp Val Tyr Ala Ile Gln Leu Phe Gly Lys Lys Arg Trp Gln Leu

145 150 155 160

Thr Ala Pro Asp Phe Pro Met Pro Leu Tyr Met Gln Gln Thr Lys Asp

165 170 175

Thr Asp Ile Ser Ile Pro Glu His Ile Asp Met Asp Ile Ile Leu Glu

180 185 190

Ala Gly Asp Val Leu Tyr Ile Pro Arg Gly Trp Trp His Arg Pro Ile

195 200 205

Pro Leu Gly Cys Glu Thr Phe His Phe Ala Val Gly Thr Phe Pro Pro

210 215 220

Asn Gly Tyr Asn Tyr Leu Glu Trp Leu Met Lys Lys Phe Pro Thr Ile

225 230 235 240

Glu Ser Leu Arg His Ser Phe Ser Asp Trp Glu Gln Asp Arg Thr Arg

245 250 255

Ile Asn Asp Thr Ala Ala Gln Ile Ala Ala Met Ile Ala Asp Pro Val

260 265 270

Asn Tyr Glu Ala Phe Ser Glu Asp Phe Leu Gly Lys Glu Arg Thr Asp

275 280 285

Thr Ala Phe His Leu Glu Gln Phe Ala Asn Pro Asn Ala Thr Pro Leu

290 295 300

Ser Asp Asp Val Arg Leu Arg Leu Asn Ala Asn Asn Leu Asp Thr Leu

305 310 315 320

Glu Lys Gly Tyr Leu Ile Gly Asn Gly Met Lys Ile Ser Val Asp Glu

325 330 335

Leu Gly Lys Lys Val Leu Glu His Ile Gly Lys Asn Glu Pro Leu Leu

340 345 350

Leu Lys Asn Leu Leu Val Asn Phe Asn Gln Ala Lys His Glu Glu Val

355 360 365

Arg Lys Leu Ile Tyr Gln Leu Ile Glu Leu Asp Phe Leu Glu Ile Leu

370 375 380

<210> SEQ ID NO 62

<211> LENGTH: 717

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(714)

<400> SEQUENCE: 62

atg aat aga ccc aag caa ccc ttc ttc cgt ccc gaa gtc gcc gtt gcc 48

Met Asn Arg Pro Lys Gln Pro Phe Phe Arg Pro Glu Val Ala Val Ala

1 5 10 15

cgc caa acc agc ctg acg ggt aaa gtg att ctg aca cga ccg ttg tca 96

Arg Gln Thr Ser Leu Thr Gly Lys Val Ile Leu Thr Arg Pro Leu Ser

20 25 30

ttt tcc cta tgg acg aca ttt gca tcg ata tct gcg tta ttg att atc 144

Phe Ser Leu Trp Thr Thr Phe Ala Ser Ile Ser Ala Leu Leu Ile Ile

35 40 45

ctg ttt ttg ata ttt ggt aac tat acg cga aag aca aca gtg gag gga 192

Leu Phe Leu Ile Phe Gly Asn Tyr Thr Arg Lys Thr Thr Val Glu Gly

50 55 60

caa att tta cct gca tcg ggc gta atc agg gtg tat gca ccg gat acg 240

Gln Ile Leu Pro Ala Ser Gly Val Ile Arg Val Tyr Ala Pro Asp Thr

65 70 75 80

ggg aca att aca gcg aaa ttc gtg gaa gat gga gaa aag gtt aag gct 288

Gly Thr Ile Thr Ala Lys Phe Val Glu Asp Gly Glu Lys Val Lys Ala

85 90 95

ggc gac aag cta ttt gcg ctt tcg acc tca cgt ttc ggc gca gga gat 336

Gly Asp Lys Leu Phe Ala Leu Ser Thr Ser Arg Phe Gly Ala Gly Asp

100 105 110

agc gtg cag cag cag ttg aaa acg gag gca gtt ttg aag aaa acg ttg 384

Ser Val Gln Gln Gln Leu Lys Thr Glu Ala Val Leu Lys Lys Thr Leu

115 120 125

gca gaa cag gaa ctg ggt cgt ctg aag ctg ata cac ggg aat gaa acg 432

Ala Glu Gln Glu Leu Gly Arg Leu Lys Leu Ile His Gly Asn Glu Thr

130 135 140

cgc agc ctt aaa gca act gtc gaa cgt ttg gaa aac cag gaa ctc cat 480

Arg Ser Leu Lys Ala Thr Val Glu Arg Leu Glu Asn Gln Glu Leu His

145 150 155 160

att tcg caa cag ata gac ggt cag aaa agg cgc att aga ctt gcg gaa 528

Ile Ser Gln Gln Ile Asp Gly Gln Lys Arg Arg Ile Arg Leu Ala Glu

165 170 175

gaa atg ttg cag aaa tat cgt ttc cta tcc gcc aat gat gca gtg cca 576

Glu Met Leu Gln Lys Tyr Arg Phe Leu Ser Ala Asn Asp Ala Val Pro

180 185 190

aaa caa gaa atg atg aat gtc aag gca gag ctt tta gag cag aaa gcc 624

Lys Gln Glu Met Met Asn Val Lys Ala Glu Leu Leu Glu Gln Lys Ala

195 200 205

aaa ctt gat gcc tac cgc cga gaa gaa gtc ggg ctg ctt cag gaa atc 672

Lys Leu Asp Ala Tyr Arg Arg Glu Glu Val Gly Leu Leu Gln Glu Ile

210 215 220

cgc acg cag aat ctg aca ttg gcc agc ctc ccc caa gcg gca tga 717

Arg Thr Gln Asn Leu Thr Leu Ala Ser Leu Pro Gln Ala Ala

225 230 235

<210> SEQ ID NO 63

<211> LENGTH: 238

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 63

Met Asn Arg Pro Lys Gln Pro Phe Phe Arg Pro Glu Val Ala Val Ala

1 5 10 15

Arg Gln Thr Ser Leu Thr Gly Lys Val Ile Leu Thr Arg Pro Leu Ser

20 25 30

Phe Ser Leu Trp Thr Thr Phe Ala Ser Ile Ser Ala Leu Leu Ile Ile

35 40 45

Leu Phe Leu Ile Phe Gly Asn Tyr Thr Arg Lys Thr Thr Val Glu Gly

50 55 60

Gln Ile Leu Pro Ala Ser Gly Val Ile Arg Val Tyr Ala Pro Asp Thr

65 70 75 80

Gly Thr Ile Thr Ala Lys Phe Val Glu Asp Gly Glu Lys Val Lys Ala

85 90 95

Gly Asp Lys Leu Phe Ala Leu Ser Thr Ser Arg Phe Gly Ala Gly Asp

100 105 110

Ser Val Gln Gln Gln Leu Lys Thr Glu Ala Val Leu Lys Lys Thr Leu

115 120 125

Ala Glu Gln Glu Leu Gly Arg Leu Lys Leu Ile His Gly Asn Glu Thr

130 135 140

Arg Ser Leu Lys Ala Thr Val Glu Arg Leu Glu Asn Gln Glu Leu His

145 150 155 160

Ile Ser Gln Gln Ile Asp Gly Gln Lys Arg Arg Ile Arg Leu Ala Glu

165 170 175

Glu Met Leu Gln Lys Tyr Arg Phe Leu Ser Ala Asn Asp Ala Val Pro

180 185 190

Lys Gln Glu Met Met Asn Val Lys Ala Glu Leu Leu Glu Gln Lys Ala

195 200 205

Lys Leu Asp Ala Tyr Arg Arg Glu Glu Val Gly Leu Leu Gln Glu Ile

210 215 220

Arg Thr Gln Asn Leu Thr Leu Ala Ser Leu Pro Gln Ala Ala

225 230 235

<210> SEQ ID NO 64

<211> LENGTH: 690

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(687)

<400> SEQUENCE: 64

atg atg aat gtc gag gca gag ctt tta gag cag aaa gcc aaa ctt gat 48

Met Met Asn Val Glu Ala Glu Leu Leu Glu Gln Lys Ala Lys Leu Asp

1 5 10 15

gcc tac ggc cga gaa gaa gcc ggg ctg ctt cag gaa atc cgc acg cag 96

Ala Tyr Gly Arg Glu Glu Ala Gly Leu Leu Gln Glu Ile Arg Thr Gln

20 25 30

aat ctg aca ttg gcc agc ctc ccc aaa cgg cat gag aca gaa caa agc 144

Asn Leu Thr Leu Ala Ser Leu Pro Lys Arg His Glu Thr Glu Gln Ser

35 40 45

cag ctt gaa cgc acc atg gcc gat att tct caa gaa gtt ttg gat ttt 192

Gln Leu Glu Arg Thr Met Ala Asp Ile Ser Gln Glu Val Leu Asp Phe

50 55 60

gaa atg cgc tct gaa caa atc atc cgt gca gga cgg tcg ggt tat ata 240

Glu Met Arg Ser Glu Gln Ile Ile Arg Ala Gly Arg Ser Gly Tyr Ile

65 70 75 80

gca ata ccg aac gtc gaa gtc gga cgg cag gtt gat cct tcc aaa ctg 288

Ala Ile Pro Asn Val Glu Val Gly Arg Gln Val Asp Pro Ser Lys Leu

85 90 95

ctc ttg agc att gtt ccc gaa cgt acc gag tta tat gcc cat cta tat 336

Leu Leu Ser Ile Val Pro Glu Arg Thr Glu Leu Tyr Ala His Leu Tyr

100 105 110

atc ccc agc agt gca gca ggc ttt atc aag ccg aaa gac aag gtt gtc 384

Ile Pro Ser Ser Ala Ala Gly Phe Ile Lys Pro Lys Asp Lys Val Val

115 120 125

cta cgt tat cag gca tat ccc tat cag aaa ttc ggg ctt gct tcc ggc 432

Leu Arg Tyr Gln Ala Tyr Pro Tyr Gln Lys Phe Gly Leu Ala Ser Gly

130 135 140

agt gtc gta tca gtg gca aaa acg gca ctg ggc aga cag gaa ttg tcg 480

Ser Val Val Ser Val Ala Lys Thr Ala Leu Gly Arg Gln Glu Leu Ser

145 150 155 160

gga ttg ggc atg gta tcc tcc gat ttg gcg aag agc aac gaa cct gtt 528

Gly Leu Gly Met Val Ser Ser Asp Leu Ala Lys Ser Asn Glu Pro Val

165 170 175

tat ctc gtg aaa ata aaa ccc gac aaa cca acc atc act gca tac ggt 576

Tyr Leu Val Lys Ile Lys Pro Asp Lys Pro Thr Ile Thr Ala Tyr Gly

180 185 190

gag gaa aaa ccg ctg caa atc ggc atg acg ctg gaa gca gac atc cta 624

Glu Glu Lys Pro Leu Gln Ile Gly Met Thr Leu Glu Ala Asp Ile Leu

195 200 205

cac gag aaa cgg cgg ctg tac gaa tgg gta ttg gag ccg att tac agt 672

His Glu Lys Arg Arg Leu Tyr Glu Trp Val Leu Glu Pro Ile Tyr Ser

210 215 220

atg tcg ggc agg ttg taa 690

Met Ser Gly Arg Leu

225

<210> SEQ ID NO 65

<211> LENGTH: 229

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 65

Met Met Asn Val Glu Ala Glu Leu Leu Glu Gln Lys Ala Lys Leu Asp

1 5 10 15

Ala Tyr Gly Arg Glu Glu Ala Gly Leu Leu Gln Glu Ile Arg Thr Gln

20 25 30

Asn Leu Thr Leu Ala Ser Leu Pro Lys Arg His Glu Thr Glu Gln Ser

35 40 45

Gln Leu Glu Arg Thr Met Ala Asp Ile Ser Gln Glu Val Leu Asp Phe

50 55 60

Glu Met Arg Ser Glu Gln Ile Ile Arg Ala Gly Arg Ser Gly Tyr Ile

65 70 75 80

Ala Ile Pro Asn Val Glu Val Gly Arg Gln Val Asp Pro Ser Lys Leu

85 90 95

Leu Leu Ser Ile Val Pro Glu Arg Thr Glu Leu Tyr Ala His Leu Tyr

100 105 110

Ile Pro Ser Ser Ala Ala Gly Phe Ile Lys Pro Lys Asp Lys Val Val

115 120 125

Leu Arg Tyr Gln Ala Tyr Pro Tyr Gln Lys Phe Gly Leu Ala Ser Gly

130 135 140

Ser Val Val Ser Val Ala Lys Thr Ala Leu Gly Arg Gln Glu Leu Ser

145 150 155 160

Gly Leu Gly Met Val Ser Ser Asp Leu Ala Lys Ser Asn Glu Pro Val

165 170 175

Tyr Leu Val Lys Ile Lys Pro Asp Lys Pro Thr Ile Thr Ala Tyr Gly

180 185 190

Glu Glu Lys Pro Leu Gln Ile Gly Met Thr Leu Glu Ala Asp Ile Leu

195 200 205

His Glu Lys Arg Arg Leu Tyr Glu Trp Val Leu Glu Pro Ile Tyr Ser

210 215 220

Met Ser Gly Arg Leu

225

<210> SEQ ID NO 66

<211> LENGTH: 924

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(921)

<400> SEQUENCE: 66

atg caa tac agc aca ctg gca gga caa acc gac aac tcc ctc gtt tcc 48

Met Gln Tyr Ser Thr Leu Ala Gly Gln Thr Asp Asn Ser Leu Val Ser

1 5 10 15

aat aat ttc ggg ttt ttg cgc ctg ccg ctt aat ttt atg ccg tat gaa 96

Asn Asn Phe Gly Phe Leu Arg Leu Pro Leu Asn Phe Met Pro Tyr Glu

20 25 30

agc cat gcc gat tgg gtt att acc ggc gtg cct tat gat atg gcg gtt 144

Ser His Ala Asp Trp Val Ile Thr Gly Val Pro Tyr Asp Met Ala Val

35 40 45

tca ggg cgt tcc ggc gcg cgt ttc ggt cct gaa gcc atc cgg cgc gcc 192

Ser Gly Arg Ser Gly Ala Arg Phe Gly Pro Glu Ala Ile Arg Arg Ala

50 55 60

tcc gtc aac ctc gct tgg gag cac cgc agg ttt ccg tgg aca ttt gat 240

Ser Val Asn Leu Ala Trp Glu His Arg Arg Phe Pro Trp Thr Phe Asp

65 70 75 80

gtg cgc gaa cgc ctg aac att att gat tgc ggc gac ttg gtt ttt tct 288

Val Arg Glu Arg Leu Asn Ile Ile Asp Cys Gly Asp Leu Val Phe Ser

85 90 95

ttt ggc gac agc agg gat ttt gtc gaa aaa atg gaa gcg cac gcc ggc 336

Phe Gly Asp Ser Arg Asp Phe Val Glu Lys Met Glu Ala His Ala Gly

100 105 110

aaa tta ctt tct ttc ggc aaa cgc tgt ttg agt ttg ggc ggc gac cat 384

Lys Leu Leu Ser Phe Gly Lys Arg Cys Leu Ser Leu Gly Gly Asp His

115 120 125

ttc att acc ctc ccg ttg ttg cgc gcc cac gcc cgc tat ttc ggc aaa 432

Phe Ile Thr Leu Pro Leu Leu Arg Ala His Ala Arg Tyr Phe Gly Lys

130 135 140

ctc gca ctg att cat ttt gac gcg cac acc gac acc tac gac aac ggc 480

Leu Ala Leu Ile His Phe Asp Ala His Thr Asp Thr Tyr Asp Asn Gly

145 150 155 160

agc gaa tac gac cac ggc acg atg ttt tat acc gcc ccc aag gaa ggc 528

Ser Glu Tyr Asp His Gly Thr Met Phe Tyr Thr Ala Pro Lys Glu Gly

165 170 175

ctc atc gac ccg tcc cgt tcc gta caa atc ggc ata cgc acc gaa cac 576

Leu Ile Asp Pro Ser Arg Ser Val Gln Ile Gly Ile Arg Thr Glu His

180 185 190

agt aaa aaa ttg cct ttt act gtg ttg tcc gcc ccc aaa gtc aat gaa 624

Ser Lys Lys Leu Pro Phe Thr Val Leu Ser Ala Pro Lys Val Asn Glu

195 200 205

gac agt gtt gaa gag acc gtc cgt aaa atc aaa gaa acc gtc ggc aat 672

Asp Ser Val Glu Glu Thr Val Arg Lys Ile Lys Glu Thr Val Gly Asn

210 215 220

atg ccc gtt tac ctg act ttc gac ata gac tgt ctc gac ccg tcg ttc 720

Met Pro Val Tyr Leu Thr Phe Asp Ile Asp Cys Leu Asp Pro Ser Phe

225 230 235 240

gcc ccc ggg acc ggt acg ccc gta tgc ggc ggc ttg agc agc gac agg 768

Ala Pro Gly Thr Gly Thr Pro Val Cys Gly Gly Leu Ser Ser Asp Arg

245 250 255

gca tta aaa atc cta cgt ggg ctg acg gat ctc gac atc gtc ggt atg 816

Ala Leu Lys Ile Leu Arg Gly Leu Thr Asp Leu Asp Ile Val Gly Met

260 265 270

gat gtt gta gaa gtt gcc ccc tct tac gac caa tcc gac att acc gct 864

Asp Val Val Glu Val Ala Pro Ser Tyr Asp Gln Ser Asp Ile Thr Ala

275 280 285

ttg gcc ggc gcc aca att gcc ttg gaa atg ctt tac ctt caa ggt gcg 912

Leu Ala Gly Ala Thr Ile Ala Leu Glu Met Leu Tyr Leu Gln Gly Ala

290 295 300

aaa aag gac tga 924

Lys Lys Asp

305

<210> SEQ ID NO 67

<211> LENGTH: 307

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 67

Met Gln Tyr Ser Thr Leu Ala Gly Gln Thr Asp Asn Ser Leu Val Ser

1 5 10 15

Asn Asn Phe Gly Phe Leu Arg Leu Pro Leu Asn Phe Met Pro Tyr Glu

20 25 30

Ser His Ala Asp Trp Val Ile Thr Gly Val Pro Tyr Asp Met Ala Val

35 40 45

Ser Gly Arg Ser Gly Ala Arg Phe Gly Pro Glu Ala Ile Arg Arg Ala

50 55 60

Ser Val Asn Leu Ala Trp Glu His Arg Arg Phe Pro Trp Thr Phe Asp

65 70 75 80

Val Arg Glu Arg Leu Asn Ile Ile Asp Cys Gly Asp Leu Val Phe Ser

85 90 95

Phe Gly Asp Ser Arg Asp Phe Val Glu Lys Met Glu Ala His Ala Gly

100 105 110

Lys Leu Leu Ser Phe Gly Lys Arg Cys Leu Ser Leu Gly Gly Asp His

115 120 125

Phe Ile Thr Leu Pro Leu Leu Arg Ala His Ala Arg Tyr Phe Gly Lys

130 135 140

Leu Ala Leu Ile His Phe Asp Ala His Thr Asp Thr Tyr Asp Asn Gly

145 150 155 160

Ser Glu Tyr Asp His Gly Thr Met Phe Tyr Thr Ala Pro Lys Glu Gly

165 170 175

Leu Ile Asp Pro Ser Arg Ser Val Gln Ile Gly Ile Arg Thr Glu His

180 185 190

Ser Lys Lys Leu Pro Phe Thr Val Leu Ser Ala Pro Lys Val Asn Glu

195 200 205

Asp Ser Val Glu Glu Thr Val Arg Lys Ile Lys Glu Thr Val Gly Asn

210 215 220

Met Pro Val Tyr Leu Thr Phe Asp Ile Asp Cys Leu Asp Pro Ser Phe

225 230 235 240

Ala Pro Gly Thr Gly Thr Pro Val Cys Gly Gly Leu Ser Ser Asp Arg

245 250 255

Ala Leu Lys Ile Leu Arg Gly Leu Thr Asp Leu Asp Ile Val Gly Met

260 265 270

Asp Val Val Glu Val Ala Pro Ser Tyr Asp Gln Ser Asp Ile Thr Ala

275 280 285

Leu Ala Gly Ala Thr Ile Ala Leu Glu Met Leu Tyr Leu Gln Gly Ala

290 295 300

Lys Lys Asp

305

<210> SEQ ID NO 68

<211> LENGTH: 1404

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1401)

<400> SEQUENCE: 68

atg aca ttg ctc aat cta atg ata atg caa gat tac ggt att tcc gtt 48

Met Thr Leu Leu Asn Leu Met Ile Met Gln Asp Tyr Gly Ile Ser Val

1 5 10 15

tgc ctg aca ctg acg ccc tat ttg caa cat gaa cta ttt tcg gct atg 96

Cys Leu Thr Leu Thr Pro Tyr Leu Gln His Glu Leu Phe Ser Ala Met

20 25 30

aaa tcc tat ttt tcc aaa tat atc cta ccc gtt tca ctt ttt acc ttg 144

Lys Ser Tyr Phe Ser Lys Tyr Ile Leu Pro Val Ser Leu Phe Thr Leu

35 40 45

cca cta tcc ctt tcc cca tcc gtt tcg gct ttt acg ctg cct gaa gca 192

Pro Leu Ser Leu Ser Pro Ser Val Ser Ala Phe Thr Leu Pro Glu Ala

50 55 60

tgg cgg gcg gcg cag caa cat tcg gct gat ttt caa gcg tcc cat tac 240

Trp Arg Ala Ala Gln Gln His Ser Ala Asp Phe Gln Ala Ser His Tyr

65 70 75 80

cag cgt gat gca gtg cgc gca cgg caa caa caa gcc aag gcc gca ttc 288

Gln Arg Asp Ala Val Arg Ala Arg Gln Gln Gln Ala Lys Ala Ala Phe

85 90 95

ctt ccc cat gta tcc gcc aat gcc agc tac cag cgc cag ccg cca tcg 336

Leu Pro His Val Ser Ala Asn Ala Ser Tyr Gln Arg Gln Pro Pro Ser

100 105 110

att tct tcc acc cgc gaa aca cag gga tgg agc gtg cag gtg gga caa 384

Ile Ser Ser Thr Arg Glu Thr Gln Gly Trp Ser Val Gln Val Gly Gln

115 120 125

acc tta ttt gac gct gcc aaa ttt gca caa tac cgc caa agc agg ttc 432

Thr Leu Phe Asp Ala Ala Lys Phe Ala Gln Tyr Arg Gln Ser Arg Phe

130 135 140

gat acg cag gct gca gaa cag cgt ttc gat gcg gca cgc gaa gaa ttg 480

Asp Thr Gln Ala Ala Glu Gln Arg Phe Asp Ala Ala Arg Glu Glu Leu

145 150 155 160

ctg ttg aaa gtt gcc gaa agt tat ttc aac gtt tta ctc agc cga gac 528

Leu Leu Lys Val Ala Glu Ser Tyr Phe Asn Val Leu Leu Ser Arg Asp

165 170 175

acc gtt gcc gcc cat gcg gcg gaa aaa gag gct tat gcc cag cag gta 576

Thr Val Ala Ala His Ala Ala Glu Lys Glu Ala Tyr Ala Gln Gln Val

180 185 190

agg cag gcg cag gct tta ttc aat aaa ggt gct gcc acc gcg ctg gat 624

Arg Gln Ala Gln Ala Leu Phe Asn Lys Gly Ala Ala Thr Ala Leu Asp

195 200 205

att cac gaa gcc aaa gcc ggt tac gac aat gcc ctg gcc caa gaa atc 672

Ile His Glu Ala Lys Ala Gly Tyr Asp Asn Ala Leu Ala Gln Glu Ile

210 215 220

gcc gta ttg gct gag aaa caa acc tat gaa aac cag ttg aac gac tac 720

Ala Val Leu Ala Glu Lys Gln Thr Tyr Glu Asn Gln Leu Asn Asp Tyr

225 230 235 240

acc gac ctg gat agc aaa caa atc gag gcc ata gat acc gcc aac ctg 768

Thr Asp Leu Asp Ser Lys Gln Ile Glu Ala Ile Asp Thr Ala Asn Leu

245 250 255

ttg gca cgc tat ctg ccc aag ctg gaa cgt tac agt ctg gat gaa tgg 816

Leu Ala Arg Tyr Leu Pro Lys Leu Glu Arg Tyr Ser Leu Asp Glu Trp

260 265 270

cag cgc att gcc tta tcc aac aat cat gaa tac cgg atg cag cag ctt 864

Gln Arg Ile Ala Leu Ser Asn Asn His Glu Tyr Arg Met Gln Gln Leu

275 280 285

gcc ctg caa agc agc gga cag gcg ctt cgg gca gca cag aac agc cgc 912

Ala Leu Gln Ser Ser Gly Gln Ala Leu Arg Ala Ala Gln Asn Ser Arg

290 295 300

tat ccc acc gtt tct gcc cat gtc ggc tat cag aat aac ctc tac act 960

Tyr Pro Thr Val Ser Ala His Val Gly Tyr Gln Asn Asn Leu Tyr Thr

305 310 315 320

tca tct gcg cag aat aat gac tac cac tat cgg ggc aaa ggg atg agc 1008

Ser Ser Ala Gln Asn Asn Asp Tyr His Tyr Arg Gly Lys Gly Met Ser

325 330 335

gtc ggc gta cag ttg aat ttg ccg ctt tat acc ggc gga gaa ttg tcg 1056

Val Gly Val Gln Leu Asn Leu Pro Leu Tyr Thr Gly Gly Glu Leu Ser

340 345 350

ggc aaa atc cat gaa gcc gaa gcg caa tac ggg gcc gcc gaa gca cag 1104

Gly Lys Ile His Glu Ala Glu Ala Gln Tyr Gly Ala Ala Glu Ala Gln

355 360 365

ctg acc gca acc gag cgg cac atc aaa ctc gcc gta cgc cag gct tat 1152

Leu Thr Ala Thr Glu Arg His Ile Lys Leu Ala Val Arg Gln Ala Tyr

370 375 380

acc gaa agc ggt gcg gcg cgt tac caa atc atg gcg caa gaa cgg gtt 1200

Thr Glu Ser Gly Ala Ala Arg Tyr Gln Ile Met Ala Gln Glu Arg Val

385 390 395 400

ttg gaa agc agc cgt ttg aaa ctg aaa tcg acc gaa acc ggc caa caa 1248

Leu Glu Ser Ser Arg Leu Lys Leu Lys Ser Thr Glu Thr Gly Gln Gln

405 410 415

tac ggc atc cgc aac cgg ctg gaa gta ata cgg gcg cgg cag gaa gtc 1296

Tyr Gly Ile Arg Asn Arg Leu Glu Val Ile Arg Ala Arg Gln Glu Val

420 425 430

gcc caa gca gaa cag aaa ctg gct caa gca cgg tat aaa ttc atg ctg 1344

Ala Gln Ala Glu Gln Lys Leu Ala Gln Ala Arg Tyr Lys Phe Met Leu

435 440 445

gct tat ttg cgc ttg gtg aaa gag agc ggg tta ggg ttg gaa acg gta 1392

Ala Tyr Leu Arg Leu Val Lys Glu Ser Gly Leu Gly Leu Glu Thr Val

450 455 460

ttt gcg gaa taa 1404

Phe Ala Glu

465

<210> SEQ ID NO 69

<211> LENGTH: 467

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 69

Met Thr Leu Leu Asn Leu Met Ile Met Gln Asp Tyr Gly Ile Ser Val

1 5 10 15

Cys Leu Thr Leu Thr Pro Tyr Leu Gln His Glu Leu Phe Ser Ala Met

20 25 30

Lys Ser Tyr Phe Ser Lys Tyr Ile Leu Pro Val Ser Leu Phe Thr Leu

35 40 45

Pro Leu Ser Leu Ser Pro Ser Val Ser Ala Phe Thr Leu Pro Glu Ala

50 55 60

Trp Arg Ala Ala Gln Gln His Ser Ala Asp Phe Gln Ala Ser His Tyr

65 70 75 80

Gln Arg Asp Ala Val Arg Ala Arg Gln Gln Gln Ala Lys Ala Ala Phe

85 90 95

Leu Pro His Val Ser Ala Asn Ala Ser Tyr Gln Arg Gln Pro Pro Ser

100 105 110

Ile Ser Ser Thr Arg Glu Thr Gln Gly Trp Ser Val Gln Val Gly Gln

115 120 125

Thr Leu Phe Asp Ala Ala Lys Phe Ala Gln Tyr Arg Gln Ser Arg Phe

130 135 140

Asp Thr Gln Ala Ala Glu Gln Arg Phe Asp Ala Ala Arg Glu Glu Leu

145 150 155 160

Leu Leu Lys Val Ala Glu Ser Tyr Phe Asn Val Leu Leu Ser Arg Asp

165 170 175

Thr Val Ala Ala His Ala Ala Glu Lys Glu Ala Tyr Ala Gln Gln Val

180 185 190

Arg Gln Ala Gln Ala Leu Phe Asn Lys Gly Ala Ala Thr Ala Leu Asp

195 200 205

Ile His Glu Ala Lys Ala Gly Tyr Asp Asn Ala Leu Ala Gln Glu Ile

210 215 220

Ala Val Leu Ala Glu Lys Gln Thr Tyr Glu Asn Gln Leu Asn Asp Tyr

225 230 235 240

Thr Asp Leu Asp Ser Lys Gln Ile Glu Ala Ile Asp Thr Ala Asn Leu

245 250 255

Leu Ala Arg Tyr Leu Pro Lys Leu Glu Arg Tyr Ser Leu Asp Glu Trp

260 265 270

Gln Arg Ile Ala Leu Ser Asn Asn His Glu Tyr Arg Met Gln Gln Leu

275 280 285

Ala Leu Gln Ser Ser Gly Gln Ala Leu Arg Ala Ala Gln Asn Ser Arg

290 295 300

Tyr Pro Thr Val Ser Ala His Val Gly Tyr Gln Asn Asn Leu Tyr Thr

305 310 315 320

Ser Ser Ala Gln Asn Asn Asp Tyr His Tyr Arg Gly Lys Gly Met Ser

325 330 335

Val Gly Val Gln Leu Asn Leu Pro Leu Tyr Thr Gly Gly Glu Leu Ser

340 345 350

Gly Lys Ile His Glu Ala Glu Ala Gln Tyr Gly Ala Ala Glu Ala Gln

355 360 365

Leu Thr Ala Thr Glu Arg His Ile Lys Leu Ala Val Arg Gln Ala Tyr

370 375 380

Thr Glu Ser Gly Ala Ala Arg Tyr Gln Ile Met Ala Gln Glu Arg Val

385 390 395 400

Leu Glu Ser Ser Arg Leu Lys Leu Lys Ser Thr Glu Thr Gly Gln Gln

405 410 415

Tyr Gly Ile Arg Asn Arg Leu Glu Val Ile Arg Ala Arg Gln Glu Val

420 425 430

Ala Gln Ala Glu Gln Lys Leu Ala Gln Ala Arg Tyr Lys Phe Met Leu

435 440 445

Ala Tyr Leu Arg Leu Val Lys Glu Ser Gly Leu Gly Leu Glu Thr Val

450 455 460

Phe Ala Glu

465

<210> SEQ ID NO 70

<211> LENGTH: 696

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(693)

<400> SEQUENCE: 70

atg aaa caa tcc gcc cga ata aaa aat atg gat cag aca tta aaa aat 48

Met Lys Gln Ser Ala Arg Ile Lys Asn Met Asp Gln Thr Leu Lys Asn

1 5 10 15

aca ttg ggc att tgc gcg ctt tta gcc ttt tgt ttt ggc gcg gcc atc 96

Thr Leu Gly Ile Cys Ala Leu Leu Ala Phe Cys Phe Gly Ala Ala Ile

20 25 30

gca tca ggt tat cac ttg gaa tat gaa tac ggc tac cgt tat tct gcc 144

Ala Ser Gly Tyr His Leu Glu Tyr Glu Tyr Gly Tyr Arg Tyr Ser Ala

35 40 45

gtg ggc gct ttg gct tcg gtt gta ttt tta tta tta ttg gca cgc ggc 192

Val Gly Ala Leu Ala Ser Val Val Phe Leu Leu Leu Leu Ala Arg Gly

50 55 60

ttc ccg cgc gtt tct tca gtt gtt tta ctg att tac gtc ggc aca acc 240

Phe Pro Arg Val Ser Ser Val Val Leu Leu Ile Tyr Val Gly Thr Thr

65 70 75 80

gcc cta tat ttg ccg gtc ggc tgg ctg tat ggt gcg cct tct tat cag 288

Ala Leu Tyr Leu Pro Val Gly Trp Leu Tyr Gly Ala Pro Ser Tyr Gln

85 90 95

ata gtc ggt tcg ata ttg gaa agc aat cct gcc gag gcg cgt gaa ttt 336

Ile Val Gly Ser Ile Leu Glu Ser Asn Pro Ala Glu Ala Arg Glu Phe

100 105 110

gtc ggc aat ctt ccc ggg tcg ctt tat ttt gtg cag gca tta ttt ttc 384

Val Gly Asn Leu Pro Gly Ser Leu Tyr Phe Val Gln Ala Leu Phe Phe

115 120 125

att ttt ggc ttg aca gtt tgg aaa tat tgt gta tct gtg ggg gta ttt 432

Ile Phe Gly Leu Thr Val Trp Lys Tyr Cys Val Ser Val Gly Val Phe

130 135 140

gct gac gta aaa aac tat aaa cgt cgc agc aaa ata tgg ctg acc ata 480

Ala Asp Val Lys Asn Tyr Lys Arg Arg Ser Lys Ile Trp Leu Thr Ile

145 150 155 160

tta ttg act ttg att ttg tcc tgc gcg gtg atg gag aaa atc gcc ggc 528

Leu Leu Thr Leu Ile Leu Ser Cys Ala Val Met Glu Lys Ile Ala Gly

165 170 175

gat aaa gat tgg cga gaa cct gat gcc ggc ctg ttg ttg aat att ttc 576

Asp Lys Asp Trp Arg Glu Pro Asp Ala Gly Leu Leu Leu Asn Ile Phe

180 185 190

gac ctg tat tac gac ttg gct ttc cgc gcc ggc aca ata tgc cgc caa 624

Asp Leu Tyr Tyr Asp Leu Ala Phe Arg Ala Gly Thr Ile Cys Arg Gln

195 200 205

gcg cgc cca cat ttt gga agc agc aaa aaa agc gtc aac atg gca tat 672

Ala Arg Pro His Phe Gly Ser Ser Lys Lys Ser Val Asn Met Ala Tyr

210 215 220

ccg cca act tgc gcc caa gta taa 696

Pro Pro Thr Cys Ala Gln Val

225 230

<210> SEQ ID NO 71

<211> LENGTH: 231

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 71

Met Lys Gln Ser Ala Arg Ile Lys Asn Met Asp Gln Thr Leu Lys Asn

1 5 10 15

Thr Leu Gly Ile Cys Ala Leu Leu Ala Phe Cys Phe Gly Ala Ala Ile

20 25 30

Ala Ser Gly Tyr His Leu Glu Tyr Glu Tyr Gly Tyr Arg Tyr Ser Ala

35 40 45

Val Gly Ala Leu Ala Ser Val Val Phe Leu Leu Leu Leu Ala Arg Gly

50 55 60

Phe Pro Arg Val Ser Ser Val Val Leu Leu Ile Tyr Val Gly Thr Thr

65 70 75 80

Ala Leu Tyr Leu Pro Val Gly Trp Leu Tyr Gly Ala Pro Ser Tyr Gln

85 90 95

Ile Val Gly Ser Ile Leu Glu Ser Asn Pro Ala Glu Ala Arg Glu Phe

100 105 110

Val Gly Asn Leu Pro Gly Ser Leu Tyr Phe Val Gln Ala Leu Phe Phe

115 120 125

Ile Phe Gly Leu Thr Val Trp Lys Tyr Cys Val Ser Val Gly Val Phe

130 135 140

Ala Asp Val Lys Asn Tyr Lys Arg Arg Ser Lys Ile Trp Leu Thr Ile

145 150 155 160

Leu Leu Thr Leu Ile Leu Ser Cys Ala Val Met Glu Lys Ile Ala Gly

165 170 175

Asp Lys Asp Trp Arg Glu Pro Asp Ala Gly Leu Leu Leu Asn Ile Phe

180 185 190

Asp Leu Tyr Tyr Asp Leu Ala Phe Arg Ala Gly Thr Ile Cys Arg Gln

195 200 205

Ala Arg Pro His Phe Gly Ser Ser Lys Lys Ser Val Asn Met Ala Tyr

210 215 220

Pro Pro Thr Cys Ala Gln Val

225 230

<210> SEQ ID NO 72

<211> LENGTH: 2607

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(2604)

<400> SEQUENCE: 72

atg gct gcc aac caa cgt tac cgc aaa ccg ctg ccc ggt acg gat ttg 48

Met Ala Ala Asn Gln Arg Tyr Arg Lys Pro Leu Pro Gly Thr Asp Leu

1 5 10 15

gaa tac tac gac gcg cgt gcg gcg tgt gag ggc atc aaa ccc ggc tct 96

Glu Tyr Tyr Asp Ala Arg Ala Ala Cys Glu Gly Ile Lys Pro Gly Ser

20 25 30

tac gac aag ctg cct tac acg agc cgc att ttg gcg gag aat ttg gtc 144

Tyr Asp Lys Leu Pro Tyr Thr Ser Arg Ile Leu Ala Glu Asn Leu Val

35 40 45

aac cgc gcg gac aaa gtc gat ttg ccg acg ctg caa agc tgg ctg ggt 192

Asn Arg Ala Asp Lys Val Asp Leu Pro Thr Leu Gln Ser Trp Leu Gly

50 55 60

cag ctg att gag gga aaa cag gaa atc gac ttt cct tgg tat ccg gcg 240

Gln Leu Ile Glu Gly Lys Gln Glu Ile Asp Phe Pro Trp Tyr Pro Ala

65 70 75 80

cgg gtg gtg tgc cac gat att ctg ggg cag acc gcg ttg gtg gat ttg 288

Arg Val Val Cys His Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu

85 90 95

gca ggt ctg cgc gat gcg att gcc gaa aaa ggc ggc gat cct gcc aaa 336

Ala Gly Leu Arg Asp Ala Ile Ala Glu Lys Gly Gly Asp Pro Ala Lys

100 105 110

gtg aat ccg gtg gtg caa acc cag ctc atc gtc gac cac tcg ctg gcg 384

Val Asn Pro Val Val Gln Thr Gln Leu Ile Val Asp His Ser Leu Ala

115 120 125

gtg gaa tgc ggc ggc tac gac ccc gat gcg ttc cgc aaa aac cgc gaa 432

Val Glu Cys Gly Gly Tyr Asp Pro Asp Ala Phe Arg Lys Asn Arg Glu

130 135 140

atc gaa gac aga cgt aac gaa gac cgt ttc cac ttc atc aac tgg aca 480

Ile Glu Asp Arg Arg Asn Glu Asp Arg Phe His Phe Ile Asn Trp Thr

145 150 155 160

aaa acc gct ttt gaa aat gtg gac gtg att ccg gcg ggc aac ggc atc 528

Lys Thr Ala Phe Glu Asn Val Asp Val Ile Pro Ala Gly Asn Gly Ile

165 170 175

atg cac caa atc aat cta gaa aaa atg tcg ccc gtc gtc caa gtc aaa 576

Met His Gln Ile Asn Leu Glu Lys Met Ser Pro Val Val Gln Val Lys

180 185 190

aac ggc gtg gct ttc ccc gat acc tgc gtc ggc acg gat tcg cac acg 624

Asn Gly Val Ala Phe Pro Asp Thr Cys Val Gly Thr Asp Ser His Thr

195 200 205

cca cac gtc gat gcg ctg ggc gtg att tcc gtg ggc gtg ggc gga ttg 672

Pro His Val Asp Ala Leu Gly Val Ile Ser Val Gly Val Gly Gly Leu

210 215 220

gaa gcg gaa acc gta atg ctg gga cgc gcg tcc atg atg cgc ctg ccc 720

Glu Ala Glu Thr Val Met Leu Gly Arg Ala Ser Met Met Arg Leu Pro

225 230 235 240

gat att gtc ggc gtt gag ctg aac ggc aaa cgg aag gcg ggc att acg 768

Asp Ile Val Gly Val Glu Leu Asn Gly Lys Arg Lys Ala Gly Ile Thr

245 250 255

gcg acg gat att gtg ttg gca ctg acc gag ttt ctg cgc aaa gaa cgc 816

Ala Thr Asp Ile Val Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu Arg

260 265 270

gtg gtc ggg gcg ttt gtc gaa ttc ttc ggc gag ggc gcg aga agc ctg 864

Val Val Gly Ala Phe Val Glu Phe Phe Gly Glu Gly Ala Arg Ser Leu

275 280 285

tct atc ggc gac cgc gcg acc att tcc aac atg acg ccg gag ttc ggc 912

Ser Ile Gly Asp Arg Ala Thr Ile Ser Asn Met Thr Pro Glu Phe Gly

290 295 300

gcg act gcc gcg atg ttc gct att gat gag caa acc att gat tat ttg 960

Ala Thr Ala Ala Met Phe Ala Ile Asp Glu Gln Thr Ile Asp Tyr Leu

305 310 315 320

aaa ctg acc gga cgc gac gac gcg cag gtg aaa ttg gtg gaa acc tac 1008

Lys Leu Thr Gly Arg Asp Asp Ala Gln Val Lys Leu Val Glu Thr Tyr

325 330 335

gcc aaa acc gca ggc ttg tgg gca gat gcc ttg aaa acc gcc gtt tat 1056

Ala Lys Thr Ala Gly Leu Trp Ala Asp Ala Leu Lys Thr Ala Val Tyr

340 345 350

ccg cgc gtt ttg aaa ttt gat ttg agc agc gta acg cgc aat atg gca 1104

Pro Arg Val Leu Lys Phe Asp Leu Ser Ser Val Thr Arg Asn Met Ala

355 360 365

ggc ccg agc aac ccg cac gcg cgt ttt gcg acc gcc gat ttg gcc ggc 1152

Gly Pro Ser Asn Pro His Ala Arg Phe Ala Thr Ala Asp Leu Ala Gly

370 375 380

aaa ggc ttg gct aaa cct tac gaa gag cct tca gac ggc caa atg cct 1200

Lys Gly Leu Ala Lys Pro Tyr Glu Glu Pro Ser Asp Gly Gln Met Pro

385 390 395 400

gac ggt gca gtg att att gcc gcg att act tcc tgt acc aat act tcc 1248

Asp Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr Ser

405 410 415

aat ccg cgc aac gtt gtc gcc gcc gcg ctg ttg gca cgc aat gcc aac 1296

Asn Pro Arg Asn Val Val Ala Ala Ala Leu Leu Ala Arg Asn Ala Asn

420 425 430

cgc ctc ggc ttg caa cgc aaa cct tgg gtg aaa tct tcg ttt gcc ccg 1344

Arg Leu Gly Leu Gln Arg Lys Pro Trp Val Lys Ser Ser Phe Ala Pro

435 440 445

ggt tca aaa gta gcc gaa atc tat ttg aaa gaa gca gat ctg ctg ccc 1392

Gly Ser Lys Val Ala Glu Ile Tyr Leu Lys Glu Ala Asp Leu Leu Pro

450 455 460

gaa atg gaa aaa ctc ggc ttc ggt atc gtt gcc ttc gca tgt acc acc 1440

Glu Met Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr Thr

465 470 475 480

tgt aac ggc atg agc ggc gcg ctg gat ccg aaa atc cag aaa gaa atc 1488

Cys Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Lys Glu Ile

485 490 495

atc gac cgc gat ttg tac gcc acc gcc gta ttg tca ggc aac cgc aac 1536

Ile Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn

500 505 510

ttt gac ggc cgt atc cat ccg tat gcg aaa cag gct ttc ctc gct tcg 1584

Phe Asp Gly Arg Ile His Pro Tyr Ala Lys Gln Ala Phe Leu Ala Ser

515 520 525

cct ccg ttg gtc gtt gcc tac gcg ctg gca ggc agc atc cgt ttc gat 1632

Pro Pro Leu Val Val Ala Tyr Ala Leu Ala Gly Ser Ile Arg Phe Asp

530 535 540

att gaa aac gac gta ctc ggc gtt gca gac ggc aaa gaa atc cgc ctg 1680

Ile Glu Asn Asp Val Leu Gly Val Ala Asp Gly Lys Glu Ile Arg Leu

545 550 555 560

aaa gac att tgg cct acc gat gaa gaa atc gat gcc atc gtt gcc gaa 1728

Lys Asp Ile Trp Pro Thr Asp Glu Glu Ile Asp Ala Ile Val Ala Glu

565 570 575

tat gtg aaa ccg cag caa ttt cgc gac gtt tat atc ccg atg ttc gac 1776

Tyr Val Lys Pro Gln Gln Phe Arg Asp Val Tyr Ile Pro Met Phe Asp

580 585 590

acc ggc aca gcg caa aaa gca cca agc ccg ctg tac gac tgg cgt cca 1824

Thr Gly Thr Ala Gln Lys Ala Pro Ser Pro Leu Tyr Asp Trp Arg Pro

595 600 605

atg tct acc tat atc cgc cgc cca cct tac tgg gaa ggc gca ctg gca 1872

Met Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu Ala

610 615 620

ggg gaa cgc aca tta agc ggt atg cgt ccg ctg gcg att ttg ccc gac 1920

Gly Glu Arg Thr Leu Ser Gly Met Arg Pro Leu Ala Ile Leu Pro Asp

625 630 635 640

aac atc acc acc gac cat ctc tcg cca tcc aat gcg att ttg gca agc 1968

Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Leu Ala Ser

645 650 655

agt gcc gca ggc gaa tat ttg gca aaa atg ggt ttg cct gaa gaa gac 2016

Ser Ala Ala Gly Glu Tyr Leu Ala Lys Met Gly Leu Pro Glu Glu Asp

660 665 670

ttc aac tct tac gca acc cac cgt ggc gac cac ttg acc gcc caa cgc 2064

Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln Arg

675 680 685

gca acc ttc gcc aat ccg aaa ctg ttt aac gaa atg gtg aga aac gaa 2112

Ala Thr Phe Ala Asn Pro Lys Leu Phe Asn Glu Met Val Arg Asn Glu

690 695 700

gac ggc agc gta cgc caa ggt tcg ctg gca cgc gtt gaa ccc gaa ggc 2160

Asp Gly Ser Val Arg Gln Gly Ser Leu Ala Arg Val Glu Pro Glu Gly

705 710 715 720

caa acc atg cgc atg tgg gaa gcc atc gaa acc tat atg aac cgc aaa 2208

Gln Thr Met Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asn Arg Lys

725 730 735

cag ccg ctc atc atc att gcc ggc gcg gac tac ggt caa ggc tca agc 2256

Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser Ser

740 745 750

cgc gac tgg gct gca aaa ggc gta cgc ctc gcc ggc gtg gaa gcg att 2304

Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Ala Ile

755 760 765

gtt gcc gaa ggc ttc gag cgt atc cac cgc acc aac ttg atc ggt atg 2352

Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly Met

770 775 780

ggc gtg ttg ccg ctg cag ttc aaa ccg ggt acc aac cgc cac acc ctg 2400

Gly Val Leu Pro Leu Gln Phe Lys Pro Gly Thr Asn Arg His Thr Leu

785 790 795 800

caa ctg gac ggt acg gaa acc tac gac gtt gtc ggc gaa cgc aca ccg 2448

Gln Leu Asp Gly Thr Glu Thr Tyr Asp Val Val Gly Glu Arg Thr Pro

805 810 815

cgc tgc gac ctg acc ctt gtg att cac cgt aaa aac ggc gag acc gtc 2496

Arg Cys Asp Leu Thr Leu Val Ile His Arg Lys Asn Gly Glu Thr Val

820 825 830

gaa gtc ccc att acc tgc cgc ctc gat acc gca gaa gaa gtg ttg gta 2544

Glu Val Pro Ile Thr Cys Arg Leu Asp Thr Ala Glu Glu Val Leu Val

835 840 845

tat gaa gcc ggt ggc gta ttg caa cgg ttt gca cag gat ttt ttg gaa 2592

Tyr Glu Ala Gly Gly Val Leu Gln Arg Phe Ala Gln Asp Phe Leu Glu

850 855 860

ggg aac gcg gct tag 2607

Gly Asn Ala Ala

865

<210> SEQ ID NO 73

<211> LENGTH: 868

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 73

Met Ala Ala Asn Gln Arg Tyr Arg Lys Pro Leu Pro Gly Thr Asp Leu

1 5 10 15

Glu Tyr Tyr Asp Ala Arg Ala Ala Cys Glu Gly Ile Lys Pro Gly Ser

20 25 30

Tyr Asp Lys Leu Pro Tyr Thr Ser Arg Ile Leu Ala Glu Asn Leu Val

35 40 45

Asn Arg Ala Asp Lys Val Asp Leu Pro Thr Leu Gln Ser Trp Leu Gly

50 55 60

Gln Leu Ile Glu Gly Lys Gln Glu Ile Asp Phe Pro Trp Tyr Pro Ala

65 70 75 80

Arg Val Val Cys His Asp Ile Leu Gly Gln Thr Ala Leu Val Asp Leu

85 90 95

Ala Gly Leu Arg Asp Ala Ile Ala Glu Lys Gly Gly Asp Pro Ala Lys

100 105 110

Val Asn Pro Val Val Gln Thr Gln Leu Ile Val Asp His Ser Leu Ala

115 120 125

Val Glu Cys Gly Gly Tyr Asp Pro Asp Ala Phe Arg Lys Asn Arg Glu

130 135 140

Ile Glu Asp Arg Arg Asn Glu Asp Arg Phe His Phe Ile Asn Trp Thr

145 150 155 160

Lys Thr Ala Phe Glu Asn Val Asp Val Ile Pro Ala Gly Asn Gly Ile

165 170 175

Met His Gln Ile Asn Leu Glu Lys Met Ser Pro Val Val Gln Val Lys

180 185 190

Asn Gly Val Ala Phe Pro Asp Thr Cys Val Gly Thr Asp Ser His Thr

195 200 205

Pro His Val Asp Ala Leu Gly Val Ile Ser Val Gly Val Gly Gly Leu

210 215 220

Glu Ala Glu Thr Val Met Leu Gly Arg Ala Ser Met Met Arg Leu Pro

225 230 235 240

Asp Ile Val Gly Val Glu Leu Asn Gly Lys Arg Lys Ala Gly Ile Thr

245 250 255

Ala Thr Asp Ile Val Leu Ala Leu Thr Glu Phe Leu Arg Lys Glu Arg

260 265 270

Val Val Gly Ala Phe Val Glu Phe Phe Gly Glu Gly Ala Arg Ser Leu

275 280 285

Ser Ile Gly Asp Arg Ala Thr Ile Ser Asn Met Thr Pro Glu Phe Gly

290 295 300

Ala Thr Ala Ala Met Phe Ala Ile Asp Glu Gln Thr Ile Asp Tyr Leu

305 310 315 320

Lys Leu Thr Gly Arg Asp Asp Ala Gln Val Lys Leu Val Glu Thr Tyr

325 330 335

Ala Lys Thr Ala Gly Leu Trp Ala Asp Ala Leu Lys Thr Ala Val Tyr

340 345 350

Pro Arg Val Leu Lys Phe Asp Leu Ser Ser Val Thr Arg Asn Met Ala

355 360 365

Gly Pro Ser Asn Pro His Ala Arg Phe Ala Thr Ala Asp Leu Ala Gly

370 375 380

Lys Gly Leu Ala Lys Pro Tyr Glu Glu Pro Ser Asp Gly Gln Met Pro

385 390 395 400

Asp Gly Ala Val Ile Ile Ala Ala Ile Thr Ser Cys Thr Asn Thr Ser

405 410 415

Asn Pro Arg Asn Val Val Ala Ala Ala Leu Leu Ala Arg Asn Ala Asn

420 425 430

Arg Leu Gly Leu Gln Arg Lys Pro Trp Val Lys Ser Ser Phe Ala Pro

435 440 445

Gly Ser Lys Val Ala Glu Ile Tyr Leu Lys Glu Ala Asp Leu Leu Pro

450 455 460

Glu Met Glu Lys Leu Gly Phe Gly Ile Val Ala Phe Ala Cys Thr Thr

465 470 475 480

Cys Asn Gly Met Ser Gly Ala Leu Asp Pro Lys Ile Gln Lys Glu Ile

485 490 495

Ile Asp Arg Asp Leu Tyr Ala Thr Ala Val Leu Ser Gly Asn Arg Asn

500 505 510

Phe Asp Gly Arg Ile His Pro Tyr Ala Lys Gln Ala Phe Leu Ala Ser

515 520 525

Pro Pro Leu Val Val Ala Tyr Ala Leu Ala Gly Ser Ile Arg Phe Asp

530 535 540

Ile Glu Asn Asp Val Leu Gly Val Ala Asp Gly Lys Glu Ile Arg Leu

545 550 555 560

Lys Asp Ile Trp Pro Thr Asp Glu Glu Ile Asp Ala Ile Val Ala Glu

565 570 575

Tyr Val Lys Pro Gln Gln Phe Arg Asp Val Tyr Ile Pro Met Phe Asp

580 585 590

Thr Gly Thr Ala Gln Lys Ala Pro Ser Pro Leu Tyr Asp Trp Arg Pro

595 600 605

Met Ser Thr Tyr Ile Arg Arg Pro Pro Tyr Trp Glu Gly Ala Leu Ala

610 615 620

Gly Glu Arg Thr Leu Ser Gly Met Arg Pro Leu Ala Ile Leu Pro Asp

625 630 635 640

Asn Ile Thr Thr Asp His Leu Ser Pro Ser Asn Ala Ile Leu Ala Ser

645 650 655

Ser Ala Ala Gly Glu Tyr Leu Ala Lys Met Gly Leu Pro Glu Glu Asp

660 665 670

Phe Asn Ser Tyr Ala Thr His Arg Gly Asp His Leu Thr Ala Gln Arg

675 680 685

Ala Thr Phe Ala Asn Pro Lys Leu Phe Asn Glu Met Val Arg Asn Glu

690 695 700

Asp Gly Ser Val Arg Gln Gly Ser Leu Ala Arg Val Glu Pro Glu Gly

705 710 715 720

Gln Thr Met Arg Met Trp Glu Ala Ile Glu Thr Tyr Met Asn Arg Lys

725 730 735

Gln Pro Leu Ile Ile Ile Ala Gly Ala Asp Tyr Gly Gln Gly Ser Ser

740 745 750

Arg Asp Trp Ala Ala Lys Gly Val Arg Leu Ala Gly Val Glu Ala Ile

755 760 765

Val Ala Glu Gly Phe Glu Arg Ile His Arg Thr Asn Leu Ile Gly Met

770 775 780

Gly Val Leu Pro Leu Gln Phe Lys Pro Gly Thr Asn Arg His Thr Leu

785 790 795 800

Gln Leu Asp Gly Thr Glu Thr Tyr Asp Val Val Gly Glu Arg Thr Pro

805 810 815

Arg Cys Asp Leu Thr Leu Val Ile His Arg Lys Asn Gly Glu Thr Val

820 825 830

Glu Val Pro Ile Thr Cys Arg Leu Asp Thr Ala Glu Glu Val Leu Val

835 840 845

Tyr Glu Ala Gly Gly Val Leu Gln Arg Phe Ala Gln Asp Phe Leu Glu

850 855 860

Gly Asn Ala Ala

865

<210> SEQ ID NO 74

<211> LENGTH: 1170

<212> TYPE: DNA

<213> ORGANISM: Neisseria meningitidis

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(1167)

<400> SEQUENCE: 74

atg ccg caa att aaa att ccc gcc gtt tac tac cgt ggc ggt aca tca 48

Met Pro Gln Ile Lys Ile Pro Ala Val Tyr Tyr Arg Gly Gly Thr Ser

1 5 10 15

aaa ggc gtg ttt ttc aaa cgt tcc gac ctg ccc gag gcg gcg cgg gaa 96

Lys Gly Val Phe Phe Lys Arg Ser Asp Leu Pro Glu Ala Ala Arg Glu

20 25 30

gcg gga agc gca cgc gac aaa atc ctc ttg cgc gta ctc ggc agc ccg 144

Ala Gly Ser Ala Arg Asp Lys Ile Leu Leu Arg Val Leu Gly Ser Pro

35 40 45

gat ccc tac ggc aag cag ata gac ggt ttg ggc aac gcc agc tcg tcc 192

Asp Pro Tyr Gly Lys Gln Ile Asp Gly Leu Gly Asn Ala Ser Ser Ser

50 55 60

acc agc aag gcg gtg att ttg gac aag tcc gaa cgc gcc gat cac gat 240

Thr Ser Lys Ala Val Ile Leu Asp Lys Ser Glu Arg Ala Asp His Asp

65 70 75 80

gtc gat tac ctt ttc ggg caa gtt tcc atc gac aaa cct ttt gtc gat 288

Val Asp Tyr Leu Phe Gly Gln Val Ser Ile Asp Lys Pro Phe Val Asp

85 90 95

tgg agt ggc aac tgc ggc aac ctc acc gcc gcc gtg ggc gca ttt gcc 336

Trp Ser Gly Asn Cys Gly Asn Leu Thr Ala Ala Val Gly Ala Phe Ala

100 105 110

atc gag caa ggc ttg gtc gat aaa ggc aag att cct tca gac ggc atc 384

Ile Glu Gln Gly Leu Val Asp Lys Gly Lys Ile Pro Ser Asp Gly Ile

115 120 125

tgc aca gtc aaa atc tgg cag aaa aac atc ggc aaa acc att att gcc 432

Cys Thr Val Lys Ile Trp Gln Lys Asn Ile Gly Lys Thr Ile Ile Ala

130 135 140

cat gta ccg atg caa aac ggc gca gtt ttg gaa aca ggc gat ttt gag 480

His Val Pro Met Gln Asn Gly Ala Val Leu Glu Thr Gly Asp Phe Glu

145 150 155 160

ctc gac ggc gta acg ttc ccg gca gcc gaa gta caa atc gaa ttt ctt 528

Leu Asp Gly Val Thr Phe Pro Ala Ala Glu Val Gln Ile Glu Phe Leu

165 170 175

gat cca gcc gac ggc gaa ggc agt atg ttc cca acc ggc aat ttg gtc 576

Asp Pro Ala Asp Gly Glu Gly Ser Met Phe Pro Thr Gly Asn Leu Val

180 185 190

gat gaa att gat gtg ccg aat ata ggc cgt ttg aaa gcc acg ctc atc 624

Asp Glu Ile Asp Val Pro Asn Ile Gly Arg Leu Lys Ala Thr Leu Ile

195 200 205

aac gcg ggc att ccg acc gtt ttc ctg aat gcc gcc gac ttg ggc tac 672

Asn Ala Gly Ile Pro Thr Val Phe Leu Asn Ala Ala Asp Leu Gly Tyr

210 215 220

acg ggc aaa gag ttg caa gac gac atc aac aac gat gcc gca gct ttg 720

Thr Gly Lys Glu Leu Gln Asp Asp Ile Asn Asn Asp Ala Ala Ala Leu

225 230 235 240

gaa aaa ttc gag aaa atc cgc gct tac ggt gcg ctg aaa atg ggt ctg 768

Glu Lys Phe Glu Lys Ile Arg Ala Tyr Gly Ala Leu Lys Met Gly Leu

245 250 255

atc agc gac gta tcc gaa gct gcc gcc cgc gcg cac acg ccg aaa gtc 816

Ile Ser Asp Val Ser Glu Ala Ala Ala Arg Ala His Thr Pro Lys Val

260 265 270

gcc ttc gtc gcg ccc gcc gcc gat tac acc gcc tcc agt ggc aaa acc 864

Ala Phe Val Ala Pro Ala Ala Asp Tyr Thr Ala Ser Ser Gly Lys Thr

275 280 285

gtg aat gcc gcc gac atc gat ttg ctg gta cgc gcc ctg agc atg ggc 912

Val Asn Ala Ala Asp Ile Asp Leu Leu Val Arg Ala Leu Ser Met Gly

290 295 300

aaa ttg cac cac gcg atg atg ggt acc gcc tct gtt gcc att gcg acc 960

Lys Leu His His Ala Met Met Gly Thr Ala Ser Val Ala Ile Ala Thr

305 310 315 320

gcc gcc gcc gtg ccc ggt acg ctg gtc aac ctt gcc gca ggc ggc gga 1008

Ala Ala Ala Val Pro Gly Thr Leu Val Asn Leu Ala Ala Gly Gly Gly

325 330 335

acg cgt aaa gaa gtg cgc ttc ggg cat cct tcc ggc aca ttg cgc gtc 1056

Thr Arg Lys Glu Val Arg Phe Gly His Pro Ser Gly Thr Leu Arg Val

340 345 350

ggt gca gcc gcc gaa tgt cag gac gga caa tgg acg gcc acc aaa gcg 1104

Gly Ala Ala Ala Glu Cys Gln Asp Gly Gln Trp Thr Ala Thr Lys Ala

355 360 365

gtt atg agc cgc agc gca cgc gtg atg atg gaa ggt tgg gtc agg gtg 1152

Val Met Ser Arg Ser Ala Arg Val Met Met Glu Gly Trp Val Arg Val

370 375 380

ccg gaa gat tgt ttt taa 1170

Pro Glu Asp Cys Phe

385

<210> SEQ ID NO 75

<211> LENGTH: 389

<212> TYPE: PRT

<213> ORGANISM: Neisseria meningitidis

<400> SEQUENCE: 75

Met Pro Gln Ile Lys Ile Pro Ala Val Tyr Tyr Arg Gly Gly Thr Ser

1 5 10 15

Lys Gly Val Phe Phe Lys Arg Ser Asp Leu Pro Glu Ala Ala Arg Glu

20 25 30

Ala Gly Ser Ala Arg Asp Lys Ile Leu Leu Arg Val Leu Gly Ser Pro

35 40 45

Asp Pro Tyr Gly Lys Gln Ile Asp Gly Leu Gly Asn Ala Ser Ser Ser

50 55 60

Thr Ser Lys Ala Val Ile Leu Asp Lys Ser Glu Arg Ala Asp His Asp

65 70 75 80

Val Asp Tyr Leu Phe Gly Gln Val Ser Ile Asp Lys Pro Phe Val Asp

85 90 95

Trp Ser Gly Asn Cys Gly Asn Leu Thr Ala Ala Val Gly Ala Phe Ala

100 105 110

Ile Glu Gln Gly Leu Val Asp Lys Gly Lys Ile Pro Ser Asp Gly Ile

115 120 125

Cys Thr Val Lys Ile Trp Gln Lys Asn Ile Gly Lys Thr Ile Ile Ala

130 135 140

His Val Pro Met Gln Asn Gly Ala Val Leu Glu Thr Gly Asp Phe Glu

145 150 155 160

Leu Asp Gly Val Thr Phe Pro Ala Ala Glu Val Gln Ile Glu Phe Leu

165 170 175

Asp Pro Ala Asp Gly Glu Gly Ser Met Phe Pro Thr Gly Asn Leu Val

180 185 190

Asp Glu Ile Asp Val Pro Asn Ile Gly Arg Leu Lys Ala Thr Leu Ile

195 200 205

Asn Ala Gly Ile Pro Thr Val Phe Leu Asn Ala Ala Asp Leu Gly Tyr

210 215 220

Thr Gly Lys Glu Leu Gln Asp Asp Ile Asn Asn Asp Ala Ala Ala Leu

225 230 235 240

Glu Lys Phe Glu Lys Ile Arg Ala Tyr Gly Ala Leu Lys Met Gly Leu

245 250 255

Ile Ser Asp Val Ser Glu Ala Ala Ala Arg Ala His Thr Pro Lys Val

260 265 270

Ala Phe Val Ala Pro Ala Ala Asp Tyr Thr Ala Ser Ser Gly Lys Thr

275 280 285

Val Asn Ala Ala Asp Ile Asp Leu Leu Val Arg Ala Leu Ser Met Gly

290 295 300

Lys Leu His His Ala Met Met Gly Thr Ala Ser Val Ala Ile Ala Thr

305 310 315 320

Ala Ala Ala Val Pro Gly Thr Leu Val Asn Leu Ala Ala Gly Gly Gly

325 330 335

Thr Arg Lys Glu Val Arg Phe Gly His Pro Ser Gly Thr Leu Arg Val

340 345 350

Gly Ala Ala Ala Glu Cys Gln Asp Gly Gln Trp Thr Ala Thr Lys Ala

355 360 365

Val Met Ser Arg Ser Ala Arg Val Met Met Glu Gly Trp Val Arg Val

370 375 380

Pro Glu Asp Cys Phe

385

<210> SEQ ID NO 76

<211> LENGTH: 2094

<212> TYPE: DNA

<213> ORGANISM: Neisseria gonorrhoeae

<220> FEATURE:

<221> NAME/KEY: CDS

<222> LOCATION: (1)..(2091)

<400> SEQUENCE: 76

atg aat tcg acc gca agt aaa acc ctg aaa gga ttg tcg ctg gtg ttt 48

Met Asn Ser Thr Ala Ser Lys Thr Leu Lys Gly Leu Ser Leu Val Phe

1 5 10 15

ttc gcc tct ggc ttc tgc gcc ctg att tac cag gtc agc tgg cag agg 96

Phe Ala Ser Gly Phe Cys Ala Leu Ile Tyr Gln Val Ser Trp Gln Arg

20 25 30

ctt cta ttc agc cac ata ggt atc gat ttg agt tcg att act gtc att 144

Leu Leu Phe Ser His Ile Gly Ile Asp Leu Ser Ser Ile Thr Val Ile

35 40 45

att tct gta ttt atg gtc ggc ttg ggt gta ggt gcg tat ttc ggc gga 192

Ile Ser Val Phe Met Val Gly Leu Gly Val Gly Ala Tyr Phe Gly Gly

50 55 60

cgc att gct gac cgt ttt cct tca agt atc atc ccc ctg ttt tgc atc 240

Arg Ile Ala Asp Arg Phe Pro Ser Ser Ile Ile Pro Leu Phe Cys Ile

65 70 75 80

gct gaa gta tcc atc ggt ctg ttc ggt ttg gta agc aag ggt ctg att 288

Ala Glu Val Ser Ile Gly Leu Phe Gly Leu Val Ser Lys Gly Leu Ile

85 90 95

tcc ggc ttg ggg cat ctt tta gtt gag gct gat ttg ccc atc atc gct 336

Ser Gly Leu Gly His Leu Leu Val Glu Ala Asp Leu Pro Ile Ile Ala

100 105 110

gct gcc aat ttc ctc tta ttg ctg ctt cct acc ttt atg atg ggc gcg 384

Ala Ala Asn Phe Leu Leu Leu Leu Leu Pro Thr Phe Met Met Gly Ala

115 120 125

acc ttg ccc ttg ctg acc tgt ttt ttt aac cgg aaa ata cat aat gtt 432

Thr Leu Pro Leu Leu Thr Cys Phe Phe Asn Arg Lys Ile His Asn Val

130 135 140

ggc gag tct atc ggt acc tta tat ttt ttc aac act ttg ggt gcg gca 480

Gly Glu Ser Ile Gly Thr Leu Tyr Phe Phe Asn Thr Leu Gly Ala Ala

145 150 155 160

ctc gga tcg ctt gcc gcc gcc gaa ttt ttc tac gtc ttt ttt acc ctc 528

Leu Gly Ser Leu Ala Ala Ala Glu Phe Phe Tyr Val Phe Phe Thr Leu

165 170 175

tcc caa acc att gcg ctg aca gcc tgc ctt aac ctt ctg att gct gct 576

Ser Gln Thr Ile Ala Leu Thr Ala Cys Leu Asn Leu Leu Ile Ala Ala

180 185 190

tca gta tgc tgc gtt aca gaa agg atg gat atg gtg aac act aaa ccg 624

Ser Val Cys Cys Val Thr Glu Arg Met Asp Met Val Asn Thr Lys Pro

195 200 205

aat act agt gtg att aat atg ctt tct ttc ctt acc gga tta ttg agc 672

Asn Thr Ser Val Ile Asn Met Leu Ser Phe Leu Thr Gly Leu Leu Ser

210 215 220

ttg ggt ata gaa gtc ttg tgg gta agg atg ttt tcg ttc gca gca cag 720

Leu Gly Ile Glu Val Leu Trp Val Arg Met Phe Ser Phe Ala Ala Gln

225 230 235 240

tcc gtg cct cag gca ttt tca ttt att ctt gcc tgt ttt ctg acc ggt 768

Ser Val Pro Gln Ala Phe Ser Phe Ile Leu Ala Cys Phe Leu Thr Gly

245 250 255

atc gcc gtc ggc gcg tat ttt ggc aaa cgg att tgc cgc agc cgc ttt 816

Ile Ala Val Gly Ala Tyr Phe Gly Lys Arg Ile Cys Arg Ser Arg Phe

260 265 270

gtt gat att ccc ttt atc ggg cag tgc ttc ttg tgg gcg ggt att gcc 864

Val Asp Ile Pro Phe Ile Gly Gln Cys Phe Leu Trp Ala Gly Ile Ala

275 280 285

gat ttt ttg att ttg ggt gct gcg tgg ttg ttg acg ggt ttt tcc ggt 912

Asp Phe Leu Ile Leu Gly Ala Ala Trp Leu Leu Thr Gly Phe Ser Gly

290 295 300

ttc gtc cac cac gcc ggt att ttc att acc ctg tct gcc gtc gtc agg 960

Phe Val His His Ala Gly Ile Phe Ile Thr Leu Ser Ala Val Val Arg

305 310 315 320

ggg ttg att ttc cca ctt gta cac cat gtg ggt acg gat ggc aac aaa 1008

Gly Leu Ile Phe Pro Leu Val His His Val Gly Thr Asp Gly Asn Lys

325 330 335

tcc gga cga cag gtt tcc aat gtt tat ttc gcc aac gtt gcc ggc agt 1056

Ser Gly Arg Gln Val Ser Asn Val Tyr Phe Ala Asn Val Ala Gly Ser

340 345 350

gca ttg ggt ccg gtc ctt atc ggc ttt gtg ata ctt gat ttg ttg tcc 1104

Ala Leu Gly Pro Val Leu Ile Gly Phe Val Ile Leu Asp Leu Leu Ser

355 360 365

acc caa cag att tac ctg ctc atc tgt ttg att tct gct gct gtc cct 1152

Thr Gln Gln Ile Tyr Leu Leu Ile Cys Leu Ile Ser Ala Ala Val Pro

370 375 380

ttg ttt tgt aca ctg ttc caa aaa agt ctc cga ctg aat gca gtg tcg 1200

Leu Phe Cys Thr Leu Phe Gln Lys Ser Leu Arg Leu Asn Ala Val Ser

385 390 395 400

gta gca gtt tcc cta atg ttc ggc atc ctc atg ttc cta ctg ccg gat 1248

Val Ala Val Ser Leu Met Phe Gly Ile Leu Met Phe Leu Leu Pro Asp

405 410 415

tct gtc ttt caa aat att gct ggc cgt ccg gat agg ttg att gaa aac 1296

Ser Val Phe Gln Asn Ile Ala Gly Arg Pro Asp Arg Leu Ile Glu Asn

420 425 430

aaa cac ggc att gtt gcg gtt tac cat aga gat ggt gat aag gtt gtt 1344

Lys His Gly Ile Val Ala Val Tyr His Arg Asp Gly Asp Lys Val Val

435 440 445

tat ggg gcg aat gta tac gac ggc gca tac aat acc gat ata ttc aat 1392

Tyr Gly Ala Asn Val Tyr Asp Gly Ala Tyr Asn Thr Asp Ile Phe Asn

450 455 460

agt gtc aac ggc atc gaa cgt gcc tat ctg cta ccc tcc ctg aag tcc 1440

Ser Val Asn Gly Ile Glu Arg Ala Tyr Leu Leu Pro Ser Leu Lys Ser

465 470 475 480

ggc ata cgc cgc att ttc gtc gtt gga ttg agt aca ggt tcg tgg gcg 1488

Gly Ile Arg Arg Ile Phe Val Val Gly Leu Ser Thr Gly Ser Trp Ala

485 490 495

cgc gtc ttg tct gcc att ccg gaa atg cag tcg atg atc gtt gcg gaa 1536

Arg Val Leu Ser Ala Ile Pro Glu Met Gln Ser Met Ile Val Ala Glu

500 505 510

atc aat ccg gca tac cgt agc ctt atc gcg gac gag ccg caa atc gca 1584

Ile Asn Pro Ala Tyr Arg Ser Leu Ile Ala Asp Glu Pro Gln Ile Ala

515 520 525

ccg ctt ttg cag gac aaa cgt gtt gaa att gta ttg gat gac ggt agg 1632

Pro Leu Leu Gln Asp Lys Arg Val Glu Ile Val Leu Asp Asp Gly Arg

530 535 540

aaa tgg ctg cgt cgc cat cct gat gaa aaa ttc gac ctg att ttg atg 1680

Lys Trp Leu Arg Arg His Pro Asp Glu Lys Phe Asp Leu Ile Leu Met

545 550 555 560

aat tcg act tgg tac tgg cgt gcc tat tcc act aac ctg ttg agt gcg 1728

Asn Ser Thr Trp Tyr Trp Arg Ala Tyr Ser Thr Asn Leu Leu Ser Ala

565 570 575

gaa ttt tta aaa cag gtg caa agc cac ctt acc ccg gat ggt att gta 1776

Glu Phe Leu Lys Gln Val Gln Ser His Leu Thr Pro Asp Gly Ile Val

580 585 590

atg ttt aat acc acg cac agc ccg cat gct ttt gct acc gcc gta cac 1824

Met Phe Asn Thr Thr His Ser Pro His Ala Phe Ala Thr Ala Val His

595 600 605

agt att ccc tat gca tac cgc tac ggg cat atg gta gtc ggc tcg gca 1872

Ser Ile Pro Tyr Ala Tyr Arg Tyr Gly His Met Val Val Gly Ser Ala

610 615 620

acc ccg gta gtt ttc cct aat aaa gaa ctg ctc aag caa cgc ctt tcc 1920

Thr Pro Val Val Phe Pro Asn Lys Glu Leu Leu Lys Gln Arg Leu Ser

625 630 635 640

cgg ttg att tgg ccg gaa agc ggc agg cac gta ttt gac agc agc acc 1968

Arg Leu Ile Trp Pro Glu Ser Gly Arg His Val Phe Asp Ser Ser Thr

645 650 655

gtg gat gct gca gca caa aag gtt gtc tct cgt atg ctg att cgg atg 2016

Val Asp Ala Ala Ala Gln Lys Val Val Ser Arg Met Leu Ile Arg Met

660 665 670

acg gaa cct tcg gct ggg gcg gaa gtc att act gac gat aat atg att 2064

Thr Glu Pro Ser Ala Gly Ala Glu Val Ile Thr Asp Asp Asn Met Ile

675 680 685

gta gaa tac aaa tac ggc aga ggg att taa 2094

Val Glu Tyr Lys Tyr Gly Arg Gly Ile

690 695

<210> SEQ ID NO 77

<211> LENGTH: 697

<212> TYPE: PRT

<213> ORGANISM: Neisseria gonorrhoeae

<400> SEQUENCE: 77

Met Asn Ser Thr Ala Ser Lys Thr Leu Lys Gly Leu Ser Leu Val Phe

1 5 10 15

Phe Ala Ser Gly Phe Cys Ala Leu Ile Tyr Gln Val Ser Trp Gln Arg

20 25 30

Leu Leu Phe Ser His Ile Gly Ile Asp Leu Ser Ser Ile Thr Val Ile

35 40 45

Ile Ser Val Phe Met Val Gly Leu Gly Val Gly Ala Tyr Phe Gly Gly

50 55 60

Arg Ile Ala Asp Arg Phe Pro Ser Ser Ile Ile Pro Leu Phe Cys Ile

65 70 75 80

Ala Glu Val Ser Ile Gly Leu Phe Gly Leu Val Ser Lys Gly Leu Ile

85 90 95

Ser Gly Leu Gly His Leu Leu Val Glu Ala Asp Leu Pro Ile Ile Ala

100 105 110

Ala Ala Asn Phe Leu Leu Leu Leu Leu Pro Thr Phe Met Met Gly Ala

115 120 125

Thr Leu Pro Leu Leu Thr Cys Phe Phe Asn Arg Lys Ile His Asn Val

130 135 140

Gly Glu Ser Ile Gly Thr Leu Tyr Phe Phe Asn Thr Leu Gly Ala Ala

145 150 155 160

Leu Gly Ser Leu Ala Ala Ala Glu Phe Phe Tyr Val Phe Phe Thr Leu

165 170 175

Ser Gln Thr Ile Ala Leu Thr Ala Cys Leu Asn Leu Leu Ile Ala Ala

180 185 190

Ser Val Cys Cys Val Thr Glu Arg Met Asp Met Val Asn Thr Lys Pro

195 200 205

Asn Thr Ser Val Ile Asn Met Leu Ser Phe Leu Thr Gly Leu Leu Ser

210 215 220

Leu Gly Ile Glu Val Leu Trp Val Arg Met Phe Ser Phe Ala Ala Gln

225 230 235 240

Ser Val Pro Gln Ala Phe Ser Phe Ile Leu Ala Cys Phe Leu Thr Gly

245 250 255

Ile Ala Val Gly Ala Tyr Phe Gly Lys Arg Ile Cys Arg Ser Arg Phe

260 265 270

Val Asp Ile Pro Phe Ile Gly Gln Cys Phe Leu Trp Ala Gly Ile Ala

275 280 285

Asp Phe Leu Ile Leu Gly Ala Ala Trp Leu Leu Thr Gly Phe Ser Gly

290 295 300

Phe Val His His Ala Gly Ile Phe Ile Thr Leu Ser Ala Val Val Arg

305 310 315 320

Gly Leu Ile Phe Pro Leu Val His His Val Gly Thr Asp Gly Asn Lys

325 330 335

Ser Gly Arg Gln Val Ser Asn Val Tyr Phe Ala Asn Val Ala Gly Ser

340 345 350

Ala Leu Gly Pro Val Leu Ile Gly Phe Val Ile Leu Asp Leu Leu Ser

355 360 365

Thr Gln Gln Ile Tyr Leu Leu Ile Cys Leu Ile Ser Ala Ala Val Pro

370 375 380

Leu Phe Cys Thr Leu Phe Gln Lys Ser Leu Arg Leu Asn Ala Val Ser

385 390 395 400

Val Ala Val Ser Leu Met Phe Gly Ile Leu Met Phe Leu Leu Pro Asp

405 410 415

Ser Val Phe Gln Asn Ile Ala Gly Arg Pro Asp Arg Leu Ile Glu Asn

420 425 430

Lys His Gly Ile Val Ala Val Tyr His Arg Asp Gly Asp Lys Val Val

435 440 445

Tyr Gly Ala Asn Val Tyr Asp Gly Ala Tyr Asn Thr Asp Ile Phe Asn

450 455 460

Ser Val Asn Gly Ile Glu Arg Ala Tyr Leu Leu Pro Ser Leu Lys Ser

465 470 475 480

Gly Ile Arg Arg Ile Phe Val Val Gly Leu Ser Thr Gly Ser Trp Ala

485 490 495

Arg Val Leu Ser Ala Ile Pro Glu Met Gln Ser Met Ile Val Ala Glu

500 505 510

Ile Asn Pro Ala Tyr Arg Ser Leu Ile Ala Asp Glu Pro Gln Ile Ala

515 520 525

Pro Leu Leu Gln Asp Lys Arg Val Glu Ile Val Leu Asp Asp Gly Arg

530 535 540

Lys Trp Leu Arg Arg His Pro Asp Glu Lys Phe Asp Leu Ile Leu Met

545 550 555 560

Asn Ser Thr Trp Tyr Trp Arg Ala Tyr Ser Thr Asn Leu Leu Ser Ala

565 570 575

Glu Phe Leu Lys Gln Val Gln Ser His Leu Thr Pro Asp Gly Ile Val

580 585 590

Met Phe Asn Thr Thr His Ser Pro His Ala Phe Ala Thr Ala Val His

595 600 605

Ser Ile Pro Tyr Ala Tyr Arg Tyr Gly His Met Val Val Gly Ser Ala

610 615 620

Thr Pro Val Val Phe Pro Asn Lys Glu Leu Leu Lys Gln Arg Leu Ser

625 630 635 640

Arg Leu Ile Trp Pro Glu Ser Gly Arg His Val Phe Asp Ser Ser Thr

645 650 655

Val Asp Ala Ala Ala Gln Lys Val Val Ser Arg Met Leu Ile Arg Met

660 665 670

Thr Glu Pro Ser Ala Gly Ala Glu Val Ile Thr Asp Asp Asn Met Ile

675 680 685

Val Glu Tyr Lys Tyr Gly Arg Gly Ile

690 695

<210> SEQ ID NO 78

<211> LENGTH: 39

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 78

gctctagacc accatgtctg aagaaaaatt gaaaatgag 39

<210> SEQ ID NO 79

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 79

cgggatccag aaatggctgg attcgctatc ag 32

<210> SEQ ID NO 80

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 80

gctctagacc accatgaaac acttactcat cg 32

<210> SEQ ID NO 81

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 81

cgggatccaa tacgtaggac ttgggtc 27

<210> SEQ ID NO 82

<211> LENGTH: 35

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 82

gctctagacc accatgaaaa aatccctttt cgttc 35

<210> SEQ ID NO 83

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 83

cgggatccat tgcggataaa catattccgc c 31

<210> SEQ ID NO 84

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 84

gctctagacc accatgcgaa cgaccccaac cttc 34

<210> SEQ ID NO 85

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 85

cgggatccag aaccggtagc ctacgccgac 30

<210> SEQ ID NO 86

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 86

gctctagacc accatgaaca cacgcatcat cgtttc 36

<210> SEQ ID NO 87

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 87

cgggatccag caacggcctg ccgctttaag 30

<210> SEQ ID NO 88

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 88

gctctagacc accatgctga cgtttatcgg actg 34

<210> SEQ ID NO 89

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 89

cgggatccac ggcagaggca cgattcc 27

<210> SEQ ID NO 90

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 90

gctctagacc accatgggca tccatctcga cttc 34

<210> SEQ ID NO 91

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 91

cgggatccac aaaagttcca gaaaatctaa ctc 33

<210> SEQ ID NO 92

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 92

gctctagacc accatgaata gacccaagca acc 33

<210> SEQ ID NO 93

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 93

cgggatccat gccgcttggg ggaggc 26

<210> SEQ ID NO 94

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 94

gctctagacc accatgatga atgtcgaggc agag 34

<210> SEQ ID NO 95

<211> LENGTH: 26

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 95

cgggatccac agtttgcccg acatac 26

<210> SEQ ID NO 96

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 96

gctctagacc accatgaaat tttttcctgc tcc 33

<210> SEQ ID NO 97

<211> LENGTH: 62

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 97

gaagatctag aaactgtaat tcaagttgaa ggaagatcta gaaactgtaa ttcaagttga 60

ag 62

<210> SEQ ID NO 98

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 98

gctctagacc accatgattg aatttgtccg agc 33

<210> SEQ ID NO 99

<211> LENGTH: 54

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 99

cgggatccaa ccctgcgacg agttgcgcgg gatccaaccc tgcgacgagt tgcg 54

<210> SEQ ID NO 100

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 100

gctctagacc accatgcaat acagcacact ggc 33

<210> SEQ ID NO 101

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 101

cgggatccag tcctttttcg caccttgaag 30

<210> SEQ ID NO 102

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 102

gctctagacc accatggagc agtcgggcaa attc 34

<210> SEQ ID NO 103

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 103

cgggatccaa gctgtttggc gatttcggtg 30

<210> SEQ ID NO 104

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 104

gctctagacc accatgcaaa acggcggggg aaag 34

<210> SEQ ID NO 105

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 105

cgggatccag tgcctgcgca gcttggaatc 30

<210> SEQ ID NO 106

<211> LENGTH: 40

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 106

gctctagacc accatgacat tgctcaatct aatgataatg 40

<210> SEQ ID NO 107

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 107

cgggatccat tccgcaaata cctgtttcca acc 33

<210> SEQ ID NO 108

<211> LENGTH: 30

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 108

gctctagacc accatgaaac aatccgcccg 30

<210> SEQ ID NO 109

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 109

cgggatccat acttgggcgc aacatgac 28

<210> SEQ ID NO 110

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 110

gctctagacc accatgaatg tttacggttt ccc 33

<210> SEQ ID NO 111

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 111

cgggatccat tttttagacg tatttttagt cg 32

<210> SEQ ID NO 112

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 112

gctctagacc accatgatga gtcaacactc tgcc 34

<210> SEQ ID NO 113

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 113

cgggatccat ccagtttttg ctcgaaggc 29

<210> SEQ ID NO 114

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 114

gctctagacc accatgcctt cgagcaaaaa ctgg 34

<210> SEQ ID NO 115

<211> LENGTH: 32

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 115

cgggatccat cgttcttcaa tctccacaaa cg 32

<210> SEQ ID NO 116

<211> LENGTH: 31

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 116

gctctagacc accatgcacc tatgtggaaa g 31

<210> SEQ ID NO 117

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 117

cgggatccat tcaattcgct tcaacaatg 29

<210> SEQ ID NO 118

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENC

ggactagtcc accatggctg ccaaccaacg ttaccg 36

<210> SEQ ID NO 119

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 119

gaagatctaa gccgcgttcc cttccaaaaa atc 33

<210> SEQ ID NO 120

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 120

gctctagacc accatgccgc aaattaaaat tccc 34

<210> SEQ ID NO 121

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 121

cgggatccaa aaacaatctt ccggcaccc 29

<210> SEQ ID NO 122

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 122

gctctagacc accatgcgca cgccgttttg ttg 33

<210> SEQ ID NO 123

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 123

cgggatccat tgggcaacga cgaaggcac 29

<210> SEQ ID NO 124

<211> LENGTH: 33

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 124

gctctagacc accatgagaa tagagatcac acc 33

<210> SEQ ID NO 125

<211> LENGTH: 27

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 125

cgggatccat ggctcaatcc tttctgc 27

<210> SEQ ID NO 126

<211> LENGTH: 34

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 126

gctctagacc accatgattc acgtttcggc agtg 34

<210> SEQ ID NO 127

<211> LENGTH: 29

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 127

cgggatccaa cctgcttcat gggtgattc 29

<210> SEQ ID NO 128

<211> LENGTH: 36

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 128

gctctagacc accatgaatt cgaccgcaag taaaac 36

<210> SEQ ID NO 129

<211> LENGTH: 28

<212> TYPE: DNA

<213> ORGANISM: Artificial Sequence

<220> FEATURE:

<223> OTHER INFORMATION: Description of Artificial Sequence: primer

<400> SEQUENCE: 129

cgggatccaa atccctctgc cgtatttg 28

Nucleic acids and polypeptides specific of the neisseria genus pathogenic strains

Information

Patent Number

Date Filed

Date Issued

Inventors

Original Assignees

Examiners

Agents

CPC

US Classifications

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Abstract

Description

Claims

Priority Claims (1)

PCT Information

US Referenced Citations (1)

Non-Patent Literature Citations (5)

Entry
Biotecnologia Aplicada 1996, vol. 13, 1-7.*
Rudinger et al, in “Peptide Hormones”, edited by Parsons, J.A., University Park Press, Jun. 1976, p. 6.*
Burgess et al., The Journal of Cell Biology, 111:2129-2138, 1990.*
Lazar et al., Molecular and Cellular Biology, 8(3): 1247-1252, 1988.*
Jobling et al. (Mol. Microbiol. 1991, 5(7): 1755-67.