Nucleic acids and polypeptides

Abstract

The present invention provides novel nucleic acids, novel polypeptide sequences encoded by these nucleic acids and uses thereof.

Description

1.2. SEQUENCE LISTING

The material included on the compact disk, created Feb. 7, 2001, and entitled “PT_FL.784CIP2B.071000.UPDATE” which is a 7.78 MB file that comprises the sequence listing, is hereby incorporated by reference into the application in its entirety.

2. BACKGROUND OF THE INVENTION

2.1 Technical Field

The present invention provides novel polynucleotides and proteins encoded by such polynucleotides, along with uses for these polynucleotides and proteins, for example in therapeutic, diagnostic and research methods.

2.2 Background

Technology aimed at the discovery of protein factors (including e.g., cytokines, such as lymphokines, interferons, CSFs, chemokines, and interleukins) has matured rapidly over the past decade. The now routine hybridization cloning and expression cloning techniques clone novel polynucleotides “directly” in the sense that they rely on information directly related to the discovered protein (i.e., partial DNA/amino acid sequence of the protein in the case of hybridization cloning; activity of the protein in the case of expression cloning). More recent “indirect” cloning techniques such as signal sequence cloning, which isolates DNA sequences based on the presence of a now well-recognized secretory leader sequence motif, as well as various PCR-based or low stringency hybridization-based cloning techniques, have advanced the state of the art by making available large numbers of DNA/amino acid sequences for proteins that are known to have biological activity, for example, by virtue of their secreted nature in the case of leader sequence cloning, by virtue of their cell or tissue source in the case of PCR-based techniques, or by virtue of structural similarity to other genes of known biological activity.

Identified polynucleotide and polypeptide sequences have numerous applications in, for example, diagnostics, forensics, gene mapping; identification of mutations responsible for genetic disorders or other traits, to assess biodiversity, and to produce many other types of data and products dependent on DNA and amino acid sequences.

3. SUMMARY OF THE INVENTION

The compositions of the present invention include novel isolated polypeptides, novel isolated polynucleotides encoding such polypeptides, including recombinant DNA molecules, cloned genes or degenerate variants thereof, especially naturally occurring variants such as allelic variants, antisense polynucleotide molecules, and antibodies that specifically recognize one or more epitopes present on such polypeptides, as well as hybridomas producing such antibodies.

The compositions of the present invention additionally include vectors, including expression vectors, containing the polynucleotides of the invention, cells genetically engineered to contain such polynucleotides and cells genetically engineered to express such polynucleotides.

The present invention relates to a collection or library of at least one novel nucleic acid sequence assembled from expressed sequence tags (ESTs) isolated mainly by sequencing by hybridization (SBH), and in some cases, sequences obtained from one or more public databases. The invention relates also to the proteins encoded by such polynucleotides, along with therapeutic, diagnostic and research utilities for these polynucleotides and proteins. These nucleic acid sequences are designated as SEQ ID NO: 1-1104 and are provided in the Sequence Listing. In the nucleic acids provided in the Sequence Listing, A is adenosine; C is cytosine; G is guanosine; T is thymine; and N is any of the four bases. In the amino acids provided in the Sequence Listing, * corresponds to the stop codon.

The nucleic acid sequences of the present invention also include, nucleic acid sequences that hybridize to the complement of SEQ ID NO: 1-1104 under stringent hybridization conditions; nucleic acid sequences which are allelic variants or species homologues of any of the nucleic acid sequences recited above, or nucleic acid sequences that encode a peptide comprising a specific domain or truncation of the peptides encoded by SEQ ID NO: 1-1104. A polynucleotide comprising a nucleotide sequence having at least 90% identity to an identifying sequence of SEQ ID NO: 1-1104 or a degenerate variant or fragment thereof. The identifying sequence can be 100 base pairs in length.

The nucleic acid sequences of the present invention also include the sequence information from the nucleic acid sequences of SEQ ID NO: 1-1104. The sequence information can be a segment of any one of SEQ ID NO: 1-1104 that uniquely identifies or represents the sequence information of SEQ ID NO: 1-1104.

A collection as used in this application can be a collection of only one polynucleotide. The collection of sequence information or identifing information of each sequence can be provided on a nucleic acid array. In one embodiment, segments of sequence information is provided on a nucleic acid array to detect the polynucleotide that contains the segment. The array can be designed to detect full-match or mismatch to the polynucleotide that contains the segment. The collection can also be provided in a computer-readable format.

This invention also includes the reverse or direct complement of any of the nucleic acid sequences recited above; cloning or expression vectors containing the nucleic acid sequences; and host cells or organisms transformed with these expression vectors. Nucleic acid sequences (or their reverse or direct complements) according to the invention have numerous applications in a variety of techniques known to those skilled in the art of molecular biology, such as use as hybridization probes, use as primers for PCR, use in an array, use in computer-readable media, use in sequencing full-length genes, use for chromosome and gene mapping, use in the recombinant production of protein, and use in the generation of anti-sense DNA or RNA, their chemical analogs and the like.

In a preferred embodiment, the nucleic acid sequences of SEQ ID NO: 1-1104 or novel segments or parts of the nucleic acids of the invention are used as primers in expression assays that are well known in the art. In a particularly preferred embodiment, the nucleic acid sequences of SEQ ID NO: 1-1104 or novel segments or parts of the nucleic acids provided herein are used in diagnostics for identifing expressed genes or, as well known in the art and exemplified by Vollrath et al., Science 258:52-59 (1992), as expressed sequence tags for physical mapping of the human genome.

The isolated polynucleotides of the invention include, but are not limited to, a polynucleotide comprising any one of the nucleotide sequences set forth in the SEQ ID NO: 1-1104; a polynucleotide comprising any of the full length protein coding sequences of the SEQ ID NO: 1-1104; and a polynucleotide comprising any of the nucleotide sequences of the mature protein coding sequences of the SEQ ID NO: 1-1104. The polynucleotides of the present invention also include, but are not limited to, a polynucleotide that hybridizes under stringent hybridization conditions to (a) the complement of any one of the nucleotide sequences set forth in the SEQ ID NO: 1-1104; (b) a nucleotide sequence encoding any one of the amino acid sequences set forth in the Sequence Listing; (c) a polynucleotide which is an allelic variant of any polynucleotides recited above; (d) a polynucleotide which encodes a species homolog (e.g. orthologs) of any of the proteins recited above; or (e) a polynucleotide that encodes a polypeptide comprising a specific domain or truncation of any of the polypeptides comprising an amino acid sequence set forth in the Sequence Listing.

The isolated polypeptides of the invention include, but are not limited to, a polypeptide comprising any of the amino acid sequences set forth in the Sequence Listing; or the corresponding full length or mature protein. Polypeptides of the invention also include polypeptides with biological activity that are encoded by (a) any of the polynucleotides having a nucleotide sequence set forth in the SEQ ID NO: 1-1104; or (b) polynucleotides that hybridize to the complement of the polynucleotides of (a) under stringent hybridization conditions. Biologically or immunologically active variants of any of the polypeptide sequences in the Sequence Listing, and “substantial equivalents” thereof (e.g., with at least about 65%, 70%, 75%, 80%, 85%, 90%, 95%, 98% or 99% amino acid sequence identity) that preferably retain biological activity are also contemplated. The polypeptides of the invention may be wholly or partially chemically synthesized but are preferably produced by recombinant means using the genetically engineered cells (e.g. host cells) of the invention.

The invention also provides compositions comprising a polypeptide of the invention. Polypeptide compositions of the invention may further comprise an acceptable carrier, such as a hydrophilic, e.g., pharmaceutically acceptable, carrier.

The invention also provides host cells transformed or transfected with a polynucleotide of the invention.

The invention also relates to methods for producing a polypeptide of the invention comprising growing a culture of the host cells of the invention in a suitable culture medium under conditions permitting expression of the desired polypeptide, and purifying the polypeptide from the culture or from the host cells. Preferred embodiments include those in which the protein produced by such process is a mature form of the protein.

Polynucleotides according to the invention have numerous applications in a variety of techniques known to those skilled in the art of molecular biology. These techniques include use as hybridization probes, use as oligomers, or primers, for PCR, use for chromosome and gene mapping, use in the recombinant production of protein, and use in generation of anti-sense DNA or RNA, their chemical analogs and the like. For example, when the expression of an mRNA is largely restricted to a particular cell or tissue type, polynucleotides of the invention can be used as hybridization probes to detect the presence of the particular cell or tissue niRNA in a sample using, e.g., in situ hybridization.

In other exemplary embodiments, the polynucleotides are used in diagnostics as expressed sequence tags for identifying expressed genes or, as well known in the art and exemplified by Vollrath et al., Science 258:52-59 (1992), as expressed sequence tags for physical mapping of the human genome.

The polypeptides according to the invention can be used in a variety of conventional procedures and methods that are currently applied to other proteins. For example, a polypeptide of the invention can be used to generate an antibody that specifically binds the polypeptide. Such antibodies, particularly monoclonal antibodies, are useful for detecting or quantitating the polypeptide in tissue. The polypeptides of the invention can also be used as molecular weight markers, and as a food supplement.

Methods are also provided for preventing, treating, or ameliorating a medical condition which comprises the step of administering to a mammalian subject a therapeutically effective amount of a composition comprising a polypeptide of the present invention and a pharmaceutically acceptable carrier.

In particular, the polypeptides and polynucleotides of the invention can be utilized, for example, in methods for the prevention and/or treatment of disorders involving aberrant protein expression or biological activity.

The present invention further relates to methods for detecting the presence of the polynucleotides or polypeptides of the invention in a sample. Such methods can, for example, be utilized as part of prognostic and diagnostic evaluation of disorders as recited herein and for the identification of subjects exhibiting a predisposition to such conditions. The invention provides a method for detecting the polynucleotides of the invention in a sample, comprising contacting the sample with a compound that binds to and forms a complex with the polynucleotide of interest for a period sufficient to form the complex and under conditions sufficient to form a complex and detecting the complex such that if a complex is detected, the polynucleotide of interest is detected. The invention also provides a method for detecting the polypeptides of the invention in a sample comprising contacting the sample with a compound that binds to and forms a complex with the polypeptide under conditions and for a period sufficient to form the complex and detecting the formation of the complex such that if a complex is formed, the polypeptide is detected.

The invention also provides kits comprising polynucleotide probes and/or monoclonal antibodies, and optionally quantitative standards, for carrying out methods of the invention. Furthermore, the invention provides methods for evaluating the efficacy of drugs, and monitoring the progress of patients, involved in clinical trials for the treatment of disorders as recited above.

The invention also provides methods for the identification of compounds that modulate (i.e., increase or decrease) the expression or activity of the polynucleotides and/or polypeptides of the invention. Such methods can be utilized, for example, for the identification of compounds that can ameliorate symptoms of disorders as recited herein. Such methods can include, but are not limited to, assays for identifying compounds and other substances that interact with (e.g., bind to) the polypeptides of the invention. The invention provides a method for identifying a compound that binds to the polypeptides of the invention comprising contacting the compound with a polypeptide of the invention in a cell for a time sufficient to form a polypeptide/compound complex, wherein the complex drives expression of a reporter gene sequence in the cell; and detecting the complex by detecting the reporter gene sequence expression such that if expression of the reporter gene is detected the compound the binds to a polypeptide of the invention is identified.

The methods of the invention also provides methods for treatment which involve the administration of the polynucleotides or polypeptides of the invention to individuals exhibiting symptoms or tendencies. In addition, the invention encompasses methods for treating diseases or disorders as recited herein comprising administering compounds and other substances that modulate the overall activity of the target gene products. Compounds and other substances can effect such modulation either on the level of target gene/protein expression or target protein activity.

The polypeptides of the present invention and the polynucleotides encoding them are also useful for the same functions known to one of skill in the art as the polypeptides and polynucleotides to which they have the closest homology (set forth in Table 1). If no homology is set forth for a sequence, then the polypeptides and polynucleotides of the present invention are useful for a variety of applications, as described herein, including use in arrays for detection.

4. DETAILED DESCRIPTION OF THE INVENTION

4.1 DEFINITIONS

It must be noted that as used herein and in the appended claims, the singular forms “a”, “an” and “the” include plural references unless the context clearly dictates otherwise.

The term “active” refers to those forms of the polypeptide which retain the biologic and/or immunologic activities of any naturally occurring polypeptide. According to the invention, the terms “biologically active” or “biological activity” refer to a protein or peptide having structural, regulatory or biochemical functions of a naturally occurring molecule. Likewise “immunologically active” or “immunological activity” refers to the capability of the natural, recombinant or synthetic polypeptide to induce a specific immune response in appropriate animals or cells and to bind with specific antibodies.

The term “activated cells” as used in this application are those cells which are engaged in extracellular or intracellular membrane trafficking, including the export of secretory or enzymatic molecules as part of a normal or disease process.

The terms “complementary” or “complementarity” refer to the natural binding of polynucleotides by base pairing. For example, the sequence 5′-AGT-3′ binds to the complementary sequence 3′-TCA-5′. Complementarity between two single-stranded molecules may be “partial” such that only some of the nucleic acids bind or it may be “complete” such that total complementarity exists between the single stranded molecules. The degree of complementarity between the nucleic acid strands has significant effects on the efficiency and strength of the hybridization between the nucleic acid strands.

The term “embryonic stem cells (ES)” refers to a cell that can give rise to many differentiated cell types in an embryo or an adult, including the germ cells. The term “germ line stem cells (GSCs)” refers to stem cells derived from primordial stem cells that provide a steady and continuous source of germ cells for the production of gametes. The term “primordial germ cells (PGCs)” refers to a small population of cells set aside from other cell lineages particularly from the yolk sac, mesenteries, or gonadal ridges during embryogenesis that have the potential to differentiate into germ cells and other cells. PGCs are the source from which GSCs and ES cells are derived The PGCs, the GSCs and the ES cells are capable of self-renewal. Thus these cells not only populate the germ line and give rise to a plurality of terminally differentiated cells that comprise the adult specialized organs, but are able to regenerate themselves.

The term “expression modulating fragment,” EMF, means a series of nucleotides which modulates the expression of an operably linked ORF or another EMF.

As used herein, a sequence is said to “modulate the expression of an operably linked sequence” when the expression of the sequence is altered by the presence of the EMF. EMFs include, but are not limited to, promoters, and promoter modulating sequences (inducible elements). One class of EMFs are nucleic acid fragments which induce the expression of an operably linked ORF in response to a specific regulatory factor or physiological event.

The terms “nucleotide sequence” or “nucleic acid” or “polynucleotide” or “oligonculeotide” are used interchangeably and refer to a heteropolymer of nucleotides or the sequence of these nucleotides. These phrases also refer to DNA or RNA of genomic or synthetic origin which may be single-stranded or double-stranded and may represent the sense or the antisense strand, to peptide nucleic acid (PNA) or to any DNA-like or RNA-like material. Generally, nucleic acid segments provided by this invention may be assembled from fragments of the genome and short oligonucleotide linkers, or from a series of oligonucleotides, or from individual nucleotides, to provide a synthetic nucleic acid which is capable of being expressed in a recombinant transcriptional unit comprising regulatory elements derived from a microbial or viral operon, or a eukaryotic gene.

The terms “oligonucleotide fragment” or a “polynucleotide fragment”, “portion,” or “segment” or “probe” or “primer” are used interchangeable and refer to a sequence of nucleotide residues which are at least about 5 nucleotides, more preferably at least about 7 nucleotides, more preferably at least about 9 nucleotides, more preferably at least about 11 nucleotides and most preferably at least about 17 nucleotides. The fragment is preferably less than about 500 nucleotides, preferably less than about 200 nucleotides, more preferably less than about 100 nucleotides, more preferably less than about 50 nucleotides and most preferably less than 30 nucleotides. Preferably the probe is from about 6 nucleotides to about 200 nucleotides, preferably from about 15 to about 50 nucleotides, more preferably from about 17 to 30 nucleotides and most preferably from about 20 to 25 nucleotides. Preferably the fragments can be used in polymerase chain reaction (PCR), various hybridization procedures or microarray procedures to identify or amplify identical or related parts of mRNA or DNA molecules. A fragment or segment may uniquely identify each polynucleotide sequence of the present invention. Preferably the fragment comprises a sequence substantially similar to any one of SEQ ID NOs: 1-1104.

Probes may, for example, be used to determine whether specific mRNA molecules are present in a cell or tissue or to isolate similar nucleic acid sequences from chromosomal DNA as described by Walsh et al. (Walsh, P. S. et al., 1992, PCR Methods Appl 1:241-250). They may be labeled by nick translation, Klenow fill-in reaction, PCR, or other methods well known in the art. Probes of the present invention, their preparation and/or labeling are elaborated in Sambrook, J. et al., 1989, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, NY; or Ausubel, F. M. et al., 1989, Current Protocols in Molecular Biology, John Wiley & Sons, New York N.Y., both of which are incorporated herein by reference in their entirety.

The nucleic acid sequences of the present invention also include the sequence information from the nucleic acid sequences of SEQ ID NOs: 1-1104. The sequence information can be a segment of any one of SEQ ID NOs: 1-1104 that uniquely identifies or represents the sequence information of that sequence of SEQ ID NO: 1-1104. One such segment can be a twenty-mer nucleic acid sequence because the probability that a twenty-mer is fully matched in the human genome is 1 in 300. In the human genome, there are three billion base pairs in one set of chromosomes. Because 4 20 possible twenty-mers exist, there are 300 times more twenty-mers than there are base pairs in a set of human chromosome. Using the same analysis, the probability for a seventeen-mer to be fully matched in the human genome is approximately 1 in 5. When these segments are used in arrays for expression studies, fifteen-mer segments can be used. The probability that the fifteen-mer is fully matched in the expressed sequences is also approximately one in five because expressed sequences comprise less than approximately 5% of the entire genome sequence.

Similarly, when using sequence information for detecting a single mismatch, a segment can be a twenty-five mer. The probability that the twenty-five mer would appear in a human genome with a single mismatch is calculated by multiplying the probability for a full match (1÷4 25 ) times the increased probability for mismatch at each nucleotide position (3×25). The probability that an eighteen mer with a single mismatch can be detected in an array for expression studies is approximately one in five. The probability that a twenty-mer with a single mismatch can be detected in a human genome is approximately one in five.

The term “open reading frame,” ORF, means a series of nucleotide triplets coding for amino acids without any termination codons and is a sequence translatable into protein.

The terms “operably linked” or “operably associated” refer to functionally related nucleic acid sequences. For example, a promoter is operably associated or operably linked with a coding sequence if the promoter controls the transcription of the coding sequence. While operably linked nucleic acid sequences can be contiguous and in the same reading frame, certain genetic elements e.g. repressor genes are not contiguously linked to the coding sequence but still control transcription/translation of the coding sequence.

The term “pluripotent” refers to the capability of a cell to differentiate into a number of differentiated cell types that are present in an adult organism. A pluripotent cell is restricted in its differentiation capability in comparison to a totipotent cell.

The terms “polypeptide” or “peptide” or “amino acid sequence” refer to an oligopeptide, peptide, polypeptide or protein sequence or fragment thereof and to naturally occurring or synthetic molecules. A polypeptide “fragment,” “portion,” or “segment” is a stretch of amino acid residues of at least about 5 amino acids, preferably at least about 7 amino acids, more preferably at least about 9 amino acids and most preferably at least about 17 or more amino acids. The peptide preferably is not greater than about 200 amino acids, more preferably less than 150 amino acids and most preferably less than 100 amino acids. Preferably the peptide is from about 5 to about 200 amino acids. To be active, any polypeptide must have sufficient length to display biological and/or immunological activity.

The term “naturally occurring polypeptide” refers to polypeptides produced by cells that have not been genetically engineered and specifically contemplates various polypeptides arising from post-translational modifications of the polypeptide including, but not limited to, acetylation, carboxylation, glycosylation, phosphorylation, lipidation and acylation.

The term “translated protein coding portion” means a sequence which encodes for the full length protein which may include any leader sequence or any processing sequence.

The term “mature protein coding sequence” means a sequence which encodes a peptide or protein without a signal or leader sequence. The peptide may have been produced by processing in the cell which removes any leader/signal sequence. The peptide may be produced synthetically or the protein may have been produced using a polynucleotide only encoding for the mature protein coding sequence.

The term “derivative” refers to polypeptides chemically modified by such techniques as ubiquitination, labeling (e.g., with radionuclides or various enzymes), covalent polymer attachment such as pegylation (derivatization with polyethylene glycol) and insertion or substitution by chemical synthesis of amino acids such as ornithine, which do not normally occur in human proteins.

The term “variant”(or “analog”) refers to any polypeptide differing from naturally occurring polypeptides by amino acid insertions, deletions, and substitutions, created using, e g., recombinant DNA techniques. Guidance in determining which amino acid residues may be replaced, added or deleted without abolishing activities of interest, may be found by comparing the sequence of the particular polypeptide with that of homologous peptides and minimizing the number of amino acid sequence changes made in regions of high homology (conserved regions) or by replacing amino acids with consensus sequence.

Alternatively, recombinant variants encoding these same or similar polypeptides may be synthesized or selected by making use of the “redundancy” in the genetic code. Various codon substitutions, such as the silent changes which produce various restriction sites, may be introduced to optimize cloning into a plasmid or viral vector or expression in a particular prokaryotic or eukaryotic system. Mutations in the polynucleotide sequence may be reflected in the polypeptide or domains of other peptides added to the polypeptide to modify the properties of any part of the polypeptide, to change characteristics such as ligand-binding affinities, interchain affinities, or degradation/turnover rate.

Preferably, amino acid “substitutions” are the result of replacing one amino acid with another amino acid having similar structural and/or chemical properties, i.e., conservative amino acid replacements. “Conservative” amino acid substitutions may be made on the basis of similarity in polarity, charge, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the residues involved. For example, nonpolar (hydrophobic) amino acids include alanine, leucine, isoleucine, valine, proline, phenylalanine, tryptophan, and methionine; polar neutral amino acids include glycine, serine, threonine, cysteine, tyrosine, asparagine, and glutamine; positively charged (basic) amino acids include arginine, lysine, and histidine; and negatively charged (acidic) amino acids include aspartic acid and glutamic acid. “Insertions” or “deletions” are preferably in the range of about 1 to 20 amino acids, more preferably 1 to 10 amino acids. The variation allowed may be experimentally determined by systematically making insertions, deletions, or substitutions of amino acids in a polypeptide molecule using recombinant DNA techniques and assaying the resulting recombinant variants for activity.

Alternatively, where alteration of function is desired, insertions, deletions or non-conservative alterations can be engineered to produce altered polypeptides. Such alterations can, for example, alter one or more of the biological functions or biochemical characteristics of the polypeptides of the invention. For example, such alterations may change polypeptide characteristics such as ligand-binding affinities, interchain affinities, or degradation/turnover rate. Further, such alterations can be selected so as to generate polypeptides that are better suited for expression, scale up and the like in the host cells chosen for expression. For example, cysteine residues can be deleted or substituted with another amino acid residue in order to eliminate disulfide bridges.

The terms “purified” or “substantially purified” as used herein denotes that the indicated nucleic acid or polypeptide is present in the substantial absence of other biological macromolecules, e.g., polynucleotides, proteins, and the like. In one embodiment, the polynucleotide or polypeptide is purified such that it constitutes at least 95% by weight, more preferably at least 99% by weight, of the indicated biological macromolecules present (but water, buffers, and other small molecules, especially molecules having a molecular weight of less than 1000 daltons, can be present).

The term “isolated” as used herein refers to a nucleic acid or polypeptide separated from at least one other component (e.g., nucleic acid or polypeptide) present with the nucleic acid or polypeptide in its natural source. In one embodiment, the nucleic acid or polypeptide is found in the presence of (if anything) only a solvent, buffer, ion, or other component normally present in a solution of the same. The terms “isolated” and “purified” do not encompass nucleic acids or polypeptides present in their natural source.

The term “recombinant,” when used herein to refer to a polypeptide or protein, means that a polypeptide or protein is derived from recombinant (e.g., microbial, insect, or mammalian) expression systems. “Microbial” refers to recombinant polypeptides or proteins made in bacterial or fungal (e.g., yeast) expression systems. As a product, “recombinant microbial” defines a polypeptide or protein essentially free of native endogenous substances and unaccompanied by associated native glycosylation. Polypeptides or proteins expressed in most bacterial cultures, e.g., E. coli , will be free of glycosylation modifications; polypeptides or proteins expressed in yeast will have a glycosylation pattern in general different from those expressed in mammalian cells.

The term “recombinant expression vehicle or vector” refers to a plasmid or phage or virus or vector, for expressing a polypeptide from a DNA (RNA) sequence. An expression vehicle can comprise a transcriptional unit comprising an assembly of (1) a genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers, (2) a structural or coding sequence which is transcribed into mRNA and translated into protein, and (3) appropriate transcription initiation and termination sequences. Structural units intended for use in yeast or eukaryotic expression systems preferably include a leader sequence enabling extracellular secretion of translated protein by a host cell. Alternatively, where recombinant protein is expressed without a leader or transport sequence, it may include an amino terminal methionine residue. This residue may or may not be subsequently cleaved from the expressed recombinant protein to provide a final product.

The term “recombinant expression system” means host cells which have stably integrated a recombinant transcriptional unit into chromosomal DNA or carry the recombinant transcriptional unit extrachromosomally. Recombinant expression systems as defined herein will express heterologous polypeptides or proteins upon induction of the regulatory elements linked to the DNA segment or synthetic gene to be expressed. This term also means host cells which have stably integrated a recombinant genetic element or elements having a regulatory role in gene expression, for example, promoters or enhancers. Recombinant expression systems as defined herein will express polypeptides or proteins endogenous to the cell upon induction of the regulatory elements linked to the endogenous DNA segment or gene to be expressed. The cells can be prokaryotic or eukaryotic.

The term “secreted” includes a protein that is transported across or through a membrane, including transport as a result of signal sequences in its amino acid sequence when it is expressed in a suitable host cell. “Secreted” proteins include without limitation proteins secreted wholly (e.g., soluble proteins) or partially (e.g., receptors) from the cell in which they are expressed. “Secreted” proteins also include without limitation proteins that are transported across the membrane of the endoplasmic reticulum. “Secreted” proteins are also intended to include proteins containing non-typical signal sequences (e.g. Interleukin-1 Beta, see Krasney, P. A. and Young, P. R. (1992) Cytokine 4(2):134-143) and factors released from damaged cells (e.g. Interleukin-1 Receptor Antagonist, see Arend, W. P. et. al. (1998) Annu. Rev. Immunol. 16:27-55)

Where desired, an expression vector may be designed to contain a “signal or leader sequence” which will direct the polypeptide through the membrane of a cell. Such a sequence may be naturally present on the polypeptides of the present invention or provided from heterologous protein sources by recombinant DNA techniques.

The term “stringent” is used to refer to conditions that are commonly understood in the art as stringent. Stringent conditions can include highly stringent conditions (i.e., hybridization to filter-bound DNA in 0.5 M NaHPO 4 , 7% sodium dodecyl sulfate (SDS), 1 mM EDTA at 65° C., and washing in 0.1×SSC/0.1% SDS at 68° C.), and moderately stringent conditions (i.e., washing in 0.2×SSC/0.1% SDS at 42° C.). Other exemplary hybridization conditions are described herein in the examples.

In instances of hybridization of deoxyoligonucleotides, additional exemplary stringent hybridization conditions include washing in 6×SSC/0.05% sodium pyrophosphate at 37° C. (for 14-base oligonucleotides), 48° C. (for 17-base oligos), 55° C. (for 20-base oligonucleotides), and 60° C. (for 23-base oligonucleotides).

As used herein, “substantially equivalent” can refer both to nucleotide and amino acid sequences, for example a mutant sequence, that varies from a reference sequence by one or more substitutions, deletions, or additions, the net effect of which does not result in an adverse functional dissimilarity between the reference and subject sequences. Typically, such a substantially equivalent sequence varies from one of those listed herein by no mote than about 35% (i.e., the number of individual residue substitutions, additions, and/or deletions in a substantially equivalent sequence, as compared to the corresponding reference sequence, divided by the total number of residues in the substantially equivalent sequence is about 0.35 or less). Such a sequence is said to have 65% sequence identity to the listed sequence. In one embodiment, a substantially equivalent, e.g., mutant, sequence of the invention varies from a listed sequence by no more than 30% (70% sequence identity); in a variation of this embodiment, by no more than 25% (75% sequence identity); and in a further variation of this embodiment, by no more than 20% (80% sequence identity) and in a further variation of this embodiment, by no more than 10% (90% sequence identity) and in a further variation of this embodiment, by no more that 5% (95% sequence identity). Substantially equivalent, e.g., mutant, amino acid sequences according to the invention preferably have at least.80% sequence identity with a listed amino acid sequence, more preferably at least 90% sequence identity. Substantially equivalent nucleotide sequences of the invention can have lower percent sequence identities, taking into account, for example, the redundancy or degeneracy of the genetic code. Preferably, nucleotide sequence has at least about 65% identity, more preferably at least about 75% identity, and most preferably at least about 95% identity. For the purposes of the present invention, sequences having substantially equivalent biological activity and substantially equivalent expression characteristics are considered substantially equivalent. For the purposes of determining equivalence, truncation of the mature sequence (e.g., via a mutation which creates a spurious stop codon) should be disregarded. Sequence identity may be determined, e.g., using the Jotun Hein method (Hein, J. (1990) Methods Enzymol. 183:626-645). Identity between sequences can also be determined by other methods known in the art, e.g. by varying hybridization conditions.

The term “totipotent” refers to the capability of a cell to differentiate into all of the cell types of an adult organism.

The term “transformation” means introducing DNA into a suitable host cell so that the DNA is replicable, either as an extrachromosomal element, or by chromosomal integration. The term “transfection” refers to the taking up of an expression vector by a suitable host cell, whether or not any coding sequences are in fact expressed. The term “infection” refers to the introduction of nucleic acids into a suitable host cell by use of a virus or viral vector.

As used herein, an “uptake modulating fragment,” UMF, means a series of nucleotides which mediate the uptake of a linked DNA fragment into a cell. UMFs can be readily identified using known UMFs as a target sequence or target motif with the computer-based systems described below. The presence and activity of a UMF can be confirmed by attaching the suspected UMF to a marker sequence. The resulting nucleic acid molecule is then incubated with an appropriate host under appropriate conditions and the uptake of the marker sequence is determined. As described above, a UMF will increase the frequency of uptake of a linked marker sequence.

Each of the above terms is meant to encompass all that is described for each, unless the context dictates otherwise.

4.2 NUCLEIC ACIDS OF THE INVENTION

Nucleotide sequences of the invention are set forth in the Sequence Listing.

The isolated polynucleotides of the invention include a polynucleotide comprising the nucleotide sequences of the SEQ ID NO: 1-1104; a polynucleotide encoding any one of the peptide sequences of SEQ ID NO: 1-1104; and a polynucleotide comprising the nucleotide sequence encoding the mature protein coding sequence of the polynucleotides of any one of SEQ ID NO: 1-1104. The polynucleotides of the present invention also include, but are not limited to, a polynucleotide that hybridizes under stringent conditions to (a) the complement of any of the nucleotides sequences of the SEQ ID NO: 1-1104; (b) nucleotide sequences encoding any one of the amino acid sequences set forth in the Sequence Listing; (c) a polynucleotide which is an allelic variant of any polynucleotide recited above; (d) a polynucleotide which encodes a species homolog of any of the proteins recited above; or (e) a polynucleotide that encodes a polypeptide comprising a specific domain or truncation of the polypeptides of SEQ ID NO: 1-1104. Domains of interest may depend on the nature of the encoded polypeptide; e.g., domains in receptor-like polypeptides include ligand-binding, extracellular, transmembrane, or cytoplasmic domains, or combinations thereof; domains in immunoglobulin-like proteins include the variable immunoglobulin-like domains; domains in enzyme-like polypeptides include catalytic and substrate binding domains; and domains in ligand polypeptides include receptor-binding domains.

The polynucleotides of the invention include naturally occurring or wholly or partially synthetic DNA, e.g., cDNA and genomic DNA, and RNA, e.g., mRNA. The polynucleotides may include all of the coding region of the cDNA or may represent a portion of the coding region of the cDNA.

The present invention also provides genes corresponding to the cDNA sequences disclosed herein. The corresponding genes can be isolated in accordance with known methods using the sequence information disclosed herein. Such methods include the preparation of probes or primers from the disclosed sequence information for identification and/or amplification of genes in appropriate genomic libraries or other sources of genomic materials. Further 5′ and 3′ sequence can be obtained using methods known in the art. For example, full length cDNA or genomic DNA that corresponds to any of the polynucleotides of the SEQ ID NO: 1-1104 can be obtained by screening appropriate cDNA or genomic DNA libraries under suitable hybridization conditions using any of the polynucleotides of the SEQ ID NO: 1-1104 or a portion thereof as a probe. Alternatively, the polynucleotides of the SEQ ID NO: 1-1104 may be used as the basis for suitable primer(s) that allow identification and/or amplification of genes in appropriate genomic DNA or cDNA libraries.

The nucleic acid sequences of the invention can be assembled from ESTs and sequences (including cDNA and genomic sequences) obtained from one or more public databases, such as dbEST, gbpri, and UniGene. The EST sequences can provide identifying sequence information, representative fragment or segment information, or novel segment information for the full-length gene.

The polynucleotides of the invention also provide polynucleotides including nucleotide sequences that are substantially equivalent to the polynucleotides recited above. Polynucleotides according to the invention can have, e.g., at least about 65%, at least about 70%, at least about 75%, at least about 80%, more typically at least about 90%, and even more typically at least about 95%, sequence identity to a polynucleotide recited above.

Included within the scope of the nucleic acid sequences of the invention are nucleic acid sequence fragments that hybridize under stringent conditions to any of the nucleotide sequences of the SEQ ID NO: 1-1104, or complements thereof, which fragment is greater than about 5 nucleotides, preferably 7 nucleotides, more preferably greater than 9 nucleotides and most preferably greater than 17 nucleotides. Fragments of, e.g. 15, 17, or 20 nucleotides or more that are selective for (i.e. specifically hybridize to any one of the polynucleotides of the invention) are contemplated. Probes capable of specifically hybridizing to a polynucleotide can differentiate polynucleotide sequences of the invention from other polynucleotide sequences in the same family of genes or can differentiate human genes from genes of other species, and are preferably based on unique nucleotide sequences.

The sequences falling within the scope of the present invention are not limited to these specific sequences, but also include allelic and species variations thereof. Allelic and species variations can be routinely determined by comparing the sequence provided in SEQ ID NO: 1-1104, a representative fragment thereof, or a nucleotide sequence at least 90% identical, preferably 95% identical, to SEQ ID NOs: 1-1104 with a sequence from another isolate of the same species. Furthermore, to accommodate codon variability, the invention includes nucleic acid molecules coding for the same amino acid sequences as do the specific ORFs disclosed herein. In other words, in the coding region of an ORF, substitution of one codon for another codon that encodes the same amino acid is expressly contemplated.

The nearest neighbor result for the nucleic acids of the present invention, including SEQ ID NOs: 1-1104, can be obtained by searching a database using an algorithm or a program. Preferably, a BLAST which stands for Basic Local Alignment Search Tool is used to search for local sequence alignments (Altshul, S. F. J Mol. Evol. 36 290-300 (1993) and Altschul S. F. et al. J. Mol. Biol. 21:403410 (1990)). Alternatively a FASTA version 3 search against Genpept, using Fastxy algorithm.

Species homologs (or orthologs) of the disclosed polynucleotides and proteins are also provided by the present invention. Species homologs may be isolated and identified by making suitable probes or primers from the sequences provided herein and screening a suitable nucleic acid source from the desired species.

The invention also encompasses allelic variants of the disclosed polynucleotides or proteins; that is, naturally-occurring alternative forms of the isolated polynucleotide which also encode proteins which are identical, homologous or related to that encoded by the polynucleotides.

The nucleic acid sequences of the invention are further directed to sequences which encode variants of the described nucleic acids. These amino acid sequence variants may be prepared by methods known in the art by introducing appropriate nucleotide changes into a native or variant polynucleotide. There are two variables in the construction of amino acid sequence variants: the location of the mutation and the nature of the mutation. Nucleic acids encoding the amino acid sequence variants are preferably constructed by mutating the polynucleotide to encode an amino acid sequence that does not occur in nature. These nucleic acid alterations can be made at sites that differ in the nucleic acids from different species (variable positions) or in highly conserved regions (constant regions). Sites at such locations will typically be modified in series, e.g., by substituting first with conservative choices (e.g., hydrophobic amino acid to a different hydrophobic amino acid) and then with more distant choices (e.g., hydrophobic amino acid to a charged amino acid), and then deletions or insertions may be made at the target site. Amino acid sequence deletions generally range from about 1 to 30 residues, preferably about 1 to 10 residues, and are typically contiguous. Amino acid insertions include amino- and/or carboxyl-terminal fusions ranging in length from one to one hundred or more residues, as well as intrasequence insertions of single or multiple amino acid residues. Intrasequence insertions may range generally from about 1 to 10 amino residues, preferably from 1 to 5 residues. Examples of terminal insertions include the heterologous signal sequences necessary for secretion or for intracellular targeting in different host cells and sequences such as FLAG or poly-histidine sequences useful for purifying the expressed protein.

In a preferred method, polynucleotides encoding the novel amino acid sequences are changed via site-directed mutagenesis. This method uses oligonucleotide sequences to alter a polynucleotide to encode the desired amino acid variant, as well as sufficient adjacent nucleotides on both sides of the changed amino acid to form a stable duplex on either side of the site of being changed. In general, the techniques of site-directed mutagenesis are well known to those of skill in the art and this technique is exemplified by publications such as, Edelman et al., DNA 2:183 (1983). A versatile and efficient method for producing site-specific changes in a polynucleotide sequence was published by Zoller and Smith, Nucleic Acids Res . 10:6487-6500 (1982). PCR may also be used to create amino acid sequence variants of the novel nucleic acids. When small amounts of template DNA are used as starting material, primer(s) that differs slightly in sequence from the corresponding region in the template DNA can generate the desired amino acid variant. PCR amplification results in a population of product DNA fragments that differ from the polynucleotide template encoding the polypeptide at the position specified by the primer. The product DNA fragments replace the corresponding region in the plasmid and this gives a polynucleotide encoding the desired amino acid variant.

A further technique for generating amino acid variants is the cassette mutagenesis technique described in Wells et al., Gene 34:315 (1985); and other mutagenesis techniques well known in the art, such as, for example, the techniques in Sambrook et al., supra, and Current Protocols in Molecular Biology , Ausubel et al. Due to the inherent degeneracy of the genetic code, other DNA sequences which encode substantially the same or a functionally equivalent amino acid sequence may be used in the practice of the invention for the cloning and expression of these novel nucleic acids. Such DNA sequences include those which are capable of hybridizing to the appropriate novel nucleic acid sequence under stringent conditions.

Polynucleotides encoding preferred polypeptide truncations of the invention can be used to generate polynucleotides encoding chimeric or fusion proteins comprising one or more domains of the invention and heterologous protein sequences.

The polynucleotides of the invention additionally include the complement of any of the polynucleotides recited above. The polynucleotide can be DNA (genomic, cDNA, amplified, or synthetic) or RNA. Methods and algorithms for obtaining such polynucleotides are well known to those of skill in the art and can include, for. example, methods for determining hybridization conditions that can routinely isolate polynucleotides of the desired sequence identities.

In accordance with the invention, polynucleotide sequences comprising the mature protein coding sequences corresponding to any one of SEQ ID NO: 1-1104, or functional equivalents thereof, may be used to generate recombinant DNA molecules that direct the expression of that nucleic acid, or a functional equivalent thereof, in appropriate host cells. Also included are the cDNA inserts of any of the clones identified herein.

A polynucleotide according to the invention can be joined to any of a variety of other nucleotide sequences by well-established recombinant DNA techniques (see Sambrook J et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, NY). Useful nucleotide sequences for joining to polynucleotides include an assortment of vectors, e.g., plasmids, cosmids, lambda phage derivatives, phagemids, and the like, that are well known in the art. Accordingly, the invention also provides a vector including a polynucleotide of the invention and a host cell containing the polynucleotide. In general, the vector contains an origin of replication functional in at least one organism, convenient restriction endonuclease sites, and a selectable marker for the host cell. Vectors according to the invention include expression vectors, replication vectors, probe generation vectors, and sequencing vectors. A host cell according to the invention can be a prokaryotic or eukaryotic cell and can be a unicellular organism or part of a multicellular organism.

The present invention further provides recombinant constructs comprising a nucleic acid having any of the nucleotide sequences of the SEQ ID NOs: 1-1104 or a fragment thereof or any other polynucleotides of the invention. In one embodiment, the recombinant constructs of the present invention comprise a vector, such as a plasmid or viral vector, into which a nucleic acid having any of the nucleotide sequences of the SEQ ID NOs: 1-1104 or a fragment thereof is inserted, in a forward or reverse orientation. In the case of a vector comprising one of the ORFs of the present invention, the vector may further comprise regulatory sequences, including for example, a promoter, operably linked to the ORF. Large numbers of suitable vectors and promoters are known to those of skill in the art and are commercially available for generating the recombinant constructs of the present invention. The following vectors are provided by way of example. Bacterial: pBs, phagescript, PsiX174, pBluescript SK, pBs KS, pNH8a, pNH16a, pNH18a, pNH46a (Stratagene); pTrc99A, pKK223-3, pKK233-3, pDR540, pRIT5 (Pharmacia). Eukaryotic: pWLneo, pSV2cat, pOG44, PXTI, pSG (Stratagene) pSVK3, pBPV, pMSG, pSVL (Pharmacia).

The isolated polynucleotide of the invention may be operably linked to an expression control sequence such as the pMT2 or pED expression vectors disclosed in Kaufman et al., Nucleic Acids Res. 19, 4485-4490 (1991), in order to produce the protein recombinantly. Many suitable expression control sequences are known in the art. General methods of expressing recombinant proteins are also known and are exemplified in R. Kaufman, Methods in Enzymology 185, 537-566 (1990). As defined herein “operably linked” means that the isolated polynucleotide of the invention and an expression control sequence are situated within a vector or cell in such a way that the protein is expressed by a host cell which has been transformed (transfected) with the ligated polynucleotide/expression control sequence.

Promoter regions can be selected from any desired gene using CAT (chloramphenicol transferase) vectors or other vectors with selectable markers. Two appropriate vectors are pKK232-8 and pCM7. Particular named bacterial promoters include lacI, lacZ, T3, T7, gpt, lambda PR, and trc. Eukaryotic promoters include CMV immediate early, HSV thymidine kinase, early and late SV40, LTRs from retrovirus, and mouse metallothionein-I. Selection of the appropriate vector and promoter is well within the level of ordinary skill in the art. Generally, recombinant expression vectors will include origins of replication and selectable markers permitting transformation of the host cell, e.g., the ampicillin resistance gene of E. coli and S. cerevisiae TRP1 gene, and a promoter derived from a highly-expressed gene to direct transcription of a downstream structural sequence. Such promoters can be derived from operons encoding glycolytic enzymes such as 3-phosphoglycerate kinase (PGK), a-factor, acid phosphatase, or heat shock proteins, among others. The heterologous structural sequence is assembled in appropriate phase with translation initiation and termination sequences, and preferably, a leader sequence capable of directing secretion of translated protein into the periplasmic space or extracellular medium. Optionally, the heterologous sequence can encode a fusion protein including an amino terminal identification peptide imparting desired characteristics, e.g., stabilization or simplified purification of expressed recombinant product. Useful expression vectors for bacterial use are constructed by inserting a structural DNA sequence encoding a desired protein together with suitable translation initiation and termination signals in operable reading phase with a functional promoter. The vector will comprise one or more phenotypic selectable markers and an origin of replication to ensure maintenance of the vector and to, if desirable, provide amplification within the host. Suitable prokaryotic hosts for transformation include E. coli, Bacillus subtilis, Salmonella typhimurium and various species within the genera Pseudomonas, Streptomyces , and Staphylococcus , although others may also be employed as a matter of choice.

As a representative but non-limiting example, useful expression vectors for bacterial use can comprise a selectable marker and bacterial origin of replication derived from commercially available plasmids comprising genetic elements of the well known cloning vector pBR322 (ATCC 37017). Such commercial vectors include, for example, pKK223-3 (Pharmacia Fine Chemicals, Uppsala, Sweden) and GEM 1 (Promega Biotech, Madison, Wis., USA). These pBR322 “backbone” sections are combined with an appropriate promoter and the structural sequence to be expressed. Following transformation of a suitable host strain and growth of the host strain to an appropriate cell density, the selected promoter is induced or derepressed by appropriate means (e.g., temperature shift or chemical induction) and cells are cultured for an additional period. Cells are typically harvested by centrifugation, disrupted by physical or chemical means, and the resulting crude extract retained for further purification.

Polynucleotides of the invention can also be used to induce immune responses. For example, as described in Fan et al., Nat. Biotech . 17:870-872 (1999), incorporated herein by reference, nucleic acid sequences encoding a polypeptide may be used to generate antibodies against the encoded polypeptide following topical administration of naked plasmid DNA or following injection, and preferably intramuscular injection of the DNA. The nucleic acid sequences are preferably inserted in a recombinant expression vector and may be in the form of naked DNA.

4.3 HOSTS

The present invention further provides host cells genetically engineered to contain the polynucleotides of the invention. For example, such host cells may contain nucleic acids of the invention introduced into the host cell using known transformation, transfection or infection methods. The present invention still further provides host cells genetically engineered to express the polynucleotides of the invention, wherein such polynucleotides are in operative association with a regulatory sequence heterologous to the host cell which drives expression of the polynucleotides in the cell.

Knowledge of nucleic acid sequences allows for modification of cells to permit, or increase, expression of endogenous polypeptide. Cells can be modified (e.g., by homologous recombination) to provide increased polypeptide expression by replacing, in whole or in part, the naturally occurring promoter with all or part of a heterologous promoter so that the cells express the polypeptide at higher levels. The heterologous promoter is inserted in such a manner that it is operatively linked to the encoding sequences. See, for example, PCT International Publication No. WO94/12650, PCT International Publication No. WO92/20808, and PCT International Publication No. WO91/09955. It is also contemplated that, in addition to heterologous promoter DNA, amplifiable marker DNA (e.g., ada, dhfr, and the multifunctional CAD gene which encodes carbamyl phosphate synthase, aspartate transcarbamylase, and dihydroorotase) and/or intron DNA may be inserted along with the heterologous promoter DNA. If linked to the coding sequence, amplification of the marker DNA by standard selection methods results in co-amplification of the desired protein coding sequences in the cells.

The host cell can be a higher eukaryotic host cell, such as a mammalian cell, a lower eukaryotic host cell, such as a yeast cell, or the host cell can be a prokaryotic cell, such as a bacterial cell. Introduction of the recombinant construct into the host cell can be effected by calcium phosphate transfection, DEAE, dextran mediated transfection, or electroporation (Davis, L. et al., Basic Methods in Molecular Biology (1986)). The host cells containing one of the polynucleotides of the invention, can be used in conventional manners to produce the gene product encoded by the isolated fragment (in the case of an ORF) or can be used to produce a heterologous protein under the control of the EMF.

Any host/vector system can be used to express one or more of the ORFs of the present invention. These include, but are not limited to, eukaryotic hosts such as HeLa cells, Cv-1 cell, COS cells, 293 cells, and Sf9 cells, as well as prokaryotic host such as E. coli and B. subtilis. The most preferred cells are those which do not normally express the particular polypeptide or protein or which expresses the polypeptide or protein at low natural level. Mature proteins can be expressed in mammalian cells, yeast, bacteria, or other cells under the control of appropriate promoters. Cell-free translation systems can also be employed to produce such proteins using RNAs derived from the DNA constructs of the present invention. Appropriate cloning and expression vectors for use with prokaryotic and eukaryotic hosts are described by Sambrook, et al., in Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, N.Y. (1989), the disclosure of which is hereby incorporated by reference.

Various mammalian cell culture systems can also be employed to express recombinant protein. Examples of mammalian expression systems include the COS-7 lines of monkey kidney fibroblasts, described by Gluzman, Cell 23:175 (1981). Other cell lines capable of expressing a compatible vector are, for example, the C127, monkey COS cells, Chinese Hamster Ovary (CHO) cells, human kidney 293 cells, human epidermal A431 cells, human Colo205 cells, 3T3 cells, CV-1 cells, other transformed primate cell lines, normal diploid cells, cell strains derived from in vitro culture of primary tissue, primary explants, HeLa cells, mouse L cells, BHK, HL-60, U937, HaK or Jurkat cells. Mammalian expression vectors will comprise an origin of replication, a suitable promoter and also any necessary ribosome binding sites, polyadenylation site, splice donor and acceptor sites, transcriptional termination sequences, and 5′ flanking nontranscribed sequences. DNA sequences derived from the SV40 viral genome, for example, SV40 origin, early promoter, enhancer, splice, and polyadenylation sites may be used to provide the required nontranscribed genetic elements. Recombinant polypeptides and proteins produced in bacterial culture are usually isolated by initial extraction from cell pellets, followed by one or more salting-out, aqueous ion exchange or size exclusion chromatography steps. Protein refolding steps can be used, as necessary, in completing configuration of the mature protein. Finally, high performance liquid chromatography (HPLC) can be employed for final purification steps. Microbial cells employed in expression of proteins can be disrupted by any convenient method, including freeze-thaw cycling, sonication, mechanical disruption, or use of cell lysing agents.

Alternatively, it may be possible to produce the protein in lower eukaryotes such as yeast or insects or in prokaryotes such as bacteria. Potentially suitable yeast strains include Saccharomyces cerevisiae, Schizosaccharomyces pombe, Kluyveromyces strains , Candida, or any yeast strain capable of expressing heterologous proteins. Potentially suitable bacterial strains include Escherichia coli, Bacillus subtilis, Salmonella typhimurium , or any bacterial strain capable of expressing heterologous proteins. If the protein is made in yeast or bacteria, it may be necessary to modify the protein produced therein, for example by phosphorylation or glycosylation of the appropriate sites, in order to obtain the functional protein. Such covalent attachments may be accomplished using known chemical or enzymatic methods.

In another embodiment of the present invention, cells and tissues may be engineered to express an endogenous gene comprising the polynucleotides of the invention under the control of inducible regulatory elements, in which case the regulatory sequences of the endogenous gene may be replaced by homologous recombination. As described herein, gene targeting can be used to replace a gene's existing regulatory region with a regulatory sequence isolated from a different gene or a novel regulatory sequence synthesized by genetic engineering methods. Such regulatory sequences may be comprised of promoters, enhancers, scaffold-attachment regions, negative regulatory elements, transcriptional initiation sites, regulatory protein binding sites or combinations of said sequences. Alternatively, sequences which affect the structure or stability of the RNA or protein produced may be replaced, removed, added, or otherwise modified by targeting. These sequence include polyadenylation signals, mRNA stability elements, splice sites, leader sequences for enhancing or modifying transport or secretion properties of the protein, or other sequences which alter or improve the function or stability of protein or RNA molecules.

The targeting event may be a simple insertion of the regulatory sequence, placing the gene under the control of the new regulatory sequence, e.g., inserting a new promoter or enhancer or both upstream of a gene. Alternatively, the targeting event may be a simple deletion of a regulatory element, such as the deletion of a tissue-specific negative regulatory element. Alternatively, the targeting event may replace an existing element; for example, a tissue-specific enhancer can be replaced by an enhancer that has broader or different cell-type specificity than the naturally occurring elements. Here, the naturally occurring sequences are deleted and new sequences are added. In all cases, the identification of the targeting event may be facilitated by the use of one or more selectable marker genes that are contiguous with the targeting DNA, allowing for the selection of cells in which the exogenous DNA has integrated into the host cell genome. The identification of the targeting event may also be facilitated by the use of one or more marker genes exhibiting the property of negative selection, such that the negatively selectable marker is linked to the exogenous DNA, but configured such that the negatively selectable marker flanks the targeting sequence, and such that a correct homologous recombination event with sequences in the host cell genome does not result in the stable integration of the negatively selectable marker. Markers useful for this purpose include the Herpes Simplex Virus thymidine kinase (TK) gene or the bacterial xanthine-guanine phosphoribosyl-transferase (gpt) gene.

The gene targeting or gene activation techniques which can be used in accordance with this aspect of the invention are more particularly described in U.S. Pat. No. 5,272,071 to Chappel; U.S. Pat. No. 5,578,461 to Sherwin et al.; International Application No. PCT/US92/09627 (WO93/09222) by Selden et al.; and International Application No. PCT/US90/06436 (WO91/06667) by Skoultchi et al., each of which is incorporated by reference herein in its entirety.

4.4 POLYPEPTIDES OF THE INVENTION

The isolated polypeptides of the invention include, but are not limited to, a polypeptide comprising: the amino acid sequences set forth as any one of SEQ ID NO: 1-1104 or an amino acid sequence encoded by any one of the nucleotide sequences SEQ ID NOs: 1-1104 or the corresponding full length or mature protein. Polypeptides of the invention also include polypeptides preferably with biological or immunological activity that are encoded by: (a) a polynucleotide having any one of the nucleotide sequences set forth in the SEQ ID NOs: 1-1104 or (b) polynucleotides encoding any one of the amino acid sequences set forth as SEQ ID NO: 1-1104 or (c) polynucleotides that hybridize to the complement of the polynucleotides of either (a) or (b) under stringent hybridization conditions. The invention also provides biologically active or immunologically active variants of any of the amino acid sequences set forth as SEQ ID NO: 1-1104 or the corresponding full length or mature protein; and “substantial equivalents” thereof (e.g., with at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, typically at least about 95%, more typically at least about 98%, or most typically at least about 99% amino acid identity) that retain biological activity. Polypeptides encoded by allelic variants may have a similar, increased, or decreased activity compared to polypeptides comprising SEQ ID NO: 1-1104.

Fragments of the proteins of the present invention which are capable of exhibiting biological activity are also encompassed by the present invention. Fragments of the protein may be in linear form or they may be cyclized using known methods, for example, as described in H. U. Saragovi, et al., Bio/Technology 10, 773-778 (1992) and in R. S. McDowell, et al., J. Amer. Chem. Soc. 114, 9245-9253 (1992), both of which are incorporated herein by reference. Such fragments may be fused to carrier molecules such as immunoglobulins for many purposes, including increasing the valency of protein binding sites.

The present invention also provides both full-length and mature forms (for example, without a signal sequence or precursor sequence) of the disclosed proteins. The protein coding sequence is identified in the sequence listing by translation of the disclosed nucleotide sequences. The mature form of such protein may be obtained by expression of a full-length polynucleotide in a suitable mammalian cell or other host cell. The sequence of the mature form of the protein is also determinable from the amino acid sequence of the full-length form. Where proteins of the present invention are membrane bound, soluble forms of the proteins are also provided. In such forms, part or all of the regions causing the proteins to be membrane bound are deleted so that the proteins are fully secreted from the cell in which it is expressed.

Protein compositions of the present invention may further comprise an acceptable carrier, such as a hydrophilic, e.g., pharmaceutically acceptable, carrier.

The present invention further provides isolated polypeptides encoded by the nucleic acid fragments of the present invention or by degenerate variants of the nucleic acid fragments of the present invention. By “degenerate variant” is intended nucleotide fragments which differ from a nucleic acid fragment of the present invention (e.g., an ORF) by nucleotide sequence but, due to the degeneracy of the genetic code, encode an identical polypeptide sequence. Preferred nucleic acid fragments of the present invention are the ORFs that encode proteins.

A variety of methodologies known in the art can be utilized to obtain any one of the isolated polypeptides or proteins of the present invention. At the simplest level, the amino acid sequence can be synthesized using commercially available peptide synthesizers. The synthetically-constructed protein sequences, by virtue of sharing primary, secondary or tertiary structural and/or conformational characteristics with proteins may possess biological properties in common therewith, including protein activity. This technique is particularly useful in producing small peptides and fragments of larger polypeptides. Fragments are useful, for example, in generating antibodies against the native polypeptide. Thus, they may be employed as biologically active or immunological substitutes for natural, purified proteins in screening of therapeutic compounds and in immunological processes for the development of antibodies.

The polypeptides and proteins of the present invention can alternatively be purified from cells which have been altered to express the desired polypeptide or protein. As used herein, a cell is said to be altered to express a desired polypeptide or protein when the cell, through genetic manipulation, is made to produce a polypeptide or protein which it normally does not produce or which the cell normally produces at a lower level. One skilled in the art can readily adapt procedures for introducing and expressing either recombinant or synthetic sequences into eukaryotic or prokaryotic cells in order to generate a cell which produces one of the polypeptides or proteins of the present invention.

The invention also relates to methods for producing a polypeptide comprising growing a culture of host cells of the invention in a suitable culture medium, and purifying the protein from the cells or the culture in which the cells are grown. For example, the methods of the invention include a process for producing a polypeptide in which a host cell containing a suitable expression vector that includes a polynucleotide of the invention is cultured under conditions that allow expression of the encoded polypeptide. The polypeptide can be recovered from the culture, conveniently from the culture medium, or from a lysate prepared from the host cells and further purified. Preferred embodiments include those in which the protein produced by such process is a full length or mature form of the protein.

In an alternative method, the polypeptide or protein is purified from bacterial cells which naturally produce the polypeptide or protein. One skilled in the art can readily follow known methods for isolating polypeptides and proteins in order to obtain one of the isolated polypeptides or proteins of the present invention. These include, but are not limited to, immunochromatography, HPLC, size-exclusion chromatography, ion-exchange chromatography, and immuno-affinity chromatography. See, e.g., Scopes, Protein Purification: Principles and Practice , Springer-Verlag (1994); Sambrook, et al., in Molecular Cloning: A Laboratory Manual ; Ausubel et al., Current Protocols in Molecular Biology . Polypeptide fragments that retain biological/immunological activity include fragments comprising greater than about 100 amino acids, or greater than about 200 amino acids, and fragments that encode specific protein domains.

The purified polypeptides can be used in in vitro binding assays which are well known in the art to identify molecules which bind to the polypeptides. These molecules include but are not limited to, for e.g., small molecules, molecules from combinatorial libraries, antibodies or other proteins. The molecules identified in the binding assay are then tested for antagonist or agonist activity in in vivo tissue culture or animal models that are well known in the art. In brief, the molecules are titrated into a plurality of cell cultures or animals and then tested for either cell/animal death or prolonged survival of the animal/cells.

In addition, the peptides of the invention or molecules capable of binding to the peptides may be complexed with toxins, e.g., ricin or cholera, or with other compounds that are toxic to cells. The toxin-binding molecule complex is then targeted to a tumor or other cell by the specificity of the binding molecule for SEQ ID NO: 1-1104.

The protein of the invention may also be expressed as a product of transgenic animals, e.g., as a component of the milk of transgenic cows, goats, pigs, or sheep which are characterized by somatic or germ cells containing a nucleotide sequence encoding the protein.

The proteins provided herein also include proteins characterized by amino acid sequences similar to those of purified proteins but into which modification are naturally provided or deliberately engineered. For example, modifications, in the peptide or DNA sequence, can be made by those skilled in the art using known techniques. Modifications of interest in the protein sequences may include the alteration, substitution, replacement, insertion or deletion of a selected amino acid residue in the coding sequence. For example, one or more of the cysteine residues may be deleted or replaced with another amino acid to alter the conformation of the molecule. Techniques for such alteration, substitution, replacement, insertion or deletion are well known to those skilled in the art (see, e.g., U.S. Pat. No. 4,518,584). Preferably, such alteration, substitution, replacement, insertion or deletion retains the desired activity of the protein. Regions of the protein that are important for the protein function can be determined by various methods known in the art including the alanine-scanning method which involved systematic substitution of single or strings of amino acids with alanine, followed by testing the resulting alanine-containing variant for biological activity. This type of analysis determines the importance of the substituted amino acid(s) in biological activity. Regions of the protein that are important for protein function may be determined by the eMATRIX program.

Other fragments and derivatives of the sequences of proteins which would be expected to retain protein activity in whole or in part and are useful for screening or other immunological methodologies may also be easily made by those skilled in the art given the disclosures herein. Such modifications are encompassed by the present invention.

The protein may also be. produced by operably linking the isolated polynucleotide of the invention to suitable control sequences in one or more insect expression vectors, and employing an insect expression system. Materials and methods for baculovirus/insect cell expression systems are commercially available in kit form from, e.g., Invitrogen, San Diego, Calif., U.S.A. (the MaxBat™ kit), and such methods are well known in the art, as described in Summers and Smith, Texas Agricultural Experiment Station Bulletin No. 1555 (1987), incorporated herein by reference. As used herein, an insect cell capable of expressing a polynucleotide of the present invention is “transformed.”

The protein of the invention may be prepared by culturing transformed host cells under culture conditions suitable to express the recombinant protein. The resulting expressed protein may then be purified from such culture (i.e., from culture medium or cell extracts) using known purification processes, such as gel filtration and ion exchange chromatography. The purification of the protein may also include an affinity column containing agents which will bind to the protein; one or more column steps over such affinity resins as concanavalin A-agarose, heparin-toyopearl™ or Cibacrom blue 3GA Sepharose™; one or more steps involving hydrophobic interaction chromatography using such resins as phenyl ether, butyl ether, or propyl ether; or immunoaffinity chromatography.

Alternatively, the protein of the invention may also be expressed in a form which will facilitate purification. For example, it may be expressed as a fusion protein, such as those of maltose binding protein (MBP), glutathione-S-transferase (GST) or thioredoxin (TRX), or as a His tag. Kits for expression and purification of such fusion proteins are commercially available from New England BioLab (Beverly, Mass.), Pharmacia (Piscataway, N.J.) and Invitrogen, respectively. The protein can also be tagged with an epitope and subsequently purified by using a specific antibody directed to such epitope. One such epitope (“FLAG®”) is commercially available from Kodak (New Haven, Conn.).

Finally, one or more reverse-phase high performance liquid chromatography (RP-HPLC) steps employing hydrophobic RP-HPLC media, e.g., silica gel having pendant methyl or other aliphatic groups, can be employed to further purify the protein. Some or all of the foregoing purification steps, in various combinations, can also be employed to provide a substantially homogeneous isolated recombinant protein. The protein thus purified is substantially free of other mammalian proteins and is defined in accordance with the present invention as an “isolated protein.”

The polypeptides of the invention include analogs (variants). This embraces fragments, as well as peptides in which one or more amino acids has been deleted, inserted, or substituted. Also, analogs of the polypeptides of the invention embrace fusions of the polypeptides or modifications of the polypeptides of the invention, wherein the polypeptide or analog is fused to another moiety or moieties, e.g., targeting moiety or another therapeutic agent. Such analogs may exhibit improved properties such as activity and/or stability. Examples of moieties which may be fused to the polypeptide or an analog include, for example, targeting moieties which provide for the delivery of polypeptide to pancreatic cells, e.g., antibodies to pancreatic cells, antibodies to immune cells such as T-cells, monocytes, dendritic cells, granulocytes, etc., as well as receptor and ligands expressed on pancreatic or immune cells. Other moieties which may be fused to the polypeptide include therapeutic agents which are used for treatment, for example, immunosuppressive drugs such as cyclosporin, SK506, azathioprine, CD3 antibodies and steroids. Also, polypeptides may be fused to immune modulators, and other cytokines such as alpha or beta interferon.

4.4.1 DETERMINING POLYPEPTIDE AND POLYNUCLEOTIDE IDENTITY AND SIMILARITY

Preferred identity and/or similarity are designed to give the largest match between the sequences tested. Methods to determine identity and similarity are codified in computer programs including, but are not limited to, the GCG program package, including GAP (Devereux, J., et al., Nucleic Acids Research 12(1):387 (1984); Genetics Computer Group, University of Wisconsin, Madison, Wis.), BLASTP, BLASTN, BLASTX, FASTA (Altschul, S. F. et al., J. Molec. Biol . 215:403-410 (1990), PSI-BLAST (Altschul S. F. et al., Nucleic Acids Res. vol. 25, pp. 3389-3402, herein incorporated by reference), eMatrix software (Wu et al., J. Comp. Biol., vol. 6, pp. 219-235 (1999), herein incorporated by reference), eMotif software (Nevill-Manning et al, ISMB-97, vol 4, pp. 202-209, herein incorporated by reference) and the Kyte-Doolittle hydrophobocity prediction algorithm ( J. Mol Biol , 157, pp. 105-31 (1982), incorporated herein by reference). The BLAST programs are publicly available from the National Center for Biotechnology Information (NCBI) and other sources (BLAST Manual, Altschul, S., et al. NCB NLM NIH Bethesda, Md. 20894; Altschul, S., et al., J. Mol. Biol . 215:403-410 (1990).

4.5 GENE THERAPY

Mutations in the polynucleotides of the invention gene may result in loss of normal function of the encoded protein. The invention thus provides gene therapy to restore normal activity of the polypeptides of the invention; or to treat disease states involving polypeptides of the invention. Delivery of a functional gene encoding polypeptides of the invention to appropriate cells is effected ex vivo, in situ, or in vivo by use of vectors, and more particularly viral vectors (e.g., adenovirus, adeno-associated virus, or a retrovirus), or.ex vivo by use of physical DNA transfer methods (e.g., liposomes or chemical treatments). See, for example, Anderson, Nature, supplement to vol. 392, no. 6679, pp.25-20 (1998). For additional reviews of gene therapy technology see Friedmann, Science, 244: 1275-1281 (1989); Verma, Scientific American: 68-84 (1990); and Miller, Nature, 357: 455-460 (1992). Introduction of any one of the nucleotides of the present invention or a gene encoding the polypeptides of the present invention can also be accomplished with extrachromosomal substrates (transient expression) or artificial chromosomes (stable expression). Cells may also be cultured ex vivo in the presence of proteins of the present invention in order to proliferate or to produce a desired effect on or activity in such cells. Treated cells can then be introduced in vivo for therapeutic purposes. Alternatively, it is contemplated that in other human disease states, preventing the expression of or inhibiting the activity of polypeptides of the invention will be useful in treating the disease states. It is contemplated that antisense therapy or gene therapy could be applied to negatively regulate the expression of polypeptides of the invention.

Other methods inhibiting expression of a protein include the introduction of antisense molecules to the nucleic acids of the present invention, their complements, or their translated RNA sequences, by methods known in the art. Further, the polypeptides of the present invention can be inhibited by using targeted deletion methods, or the insertion of a negative regulatory element such as a silencer, which is tissue specific.

The present invention still further provides cells genetically engineered in vivo to express the polynucleotides of the invention, wherein such polynucleotides are in operative association with a regulatory sequence heterologous to the host cell which drives expression of the polynucleotides in the cell. These methods can be used to increase or decrease the expression of the polynucleotides of the present invention.

Knowledge of DNA sequences provided by the invention allows for modification of cells to permit, increase, or decrease, expression of endogenous polypeptide. Cells can be modified (e.g., by homologous recombination) to provide increased polypeptide expression by replacing, in whole or in part, the naturally occurring promoter with all or part of a heterologous promoter so that the cells express the protein at higher levels. The heterologous promoter is inserted in such a manner that it is operatively linked to the desired protein encoding sequences. See, for example, PCT International Publication No. WO 94/12650, PCT International Publication No. WO 92/20808, and PCT International Publication No. WO 91/09955. It is also contemplated that, in addition to heterologous promoter DNA, amplifiable marker DNA (e.g., ada, dhfr, and the multifunctional CAD gene which encodes carbamyl phosphate synthase, aspartate transcarbamylase, and dihydroorotase) and/or intron DNA may be inserted along with the heterologous promoter DNA. If linked to the desired protein coding sequence, amplification of the marker DNA by standard selection methods results in co-amplification of the desired protein coding sequences in the cells.

In another embodiment of the present invention, cells and tissues may be engineered to express an endogenous gene comprising the polynucleotides of the invention under the control of inducible regulatory elements, in which case the regulatory sequences of the endogenous gene may be replaced by homologous recombination. As described herein, gene targeting can be used to replace a gene's existing regulatory region with a regulatory sequence isolated from a different gene or a novel regulatory sequence synthesized by genetic engineering methods. Such regulatory sequences may be comprised of promoters, enhancers, scaffold-attachment regions, negative regulatory elements, transcriptional initiation sites, regulatory protein binding sites or combinations of said sequences. Alternatively, sequences which affect the structure or stability of the RNA or protein produced may be replaced, removed, added, or otherwise modified by targeting. These sequences include polyadenylation signals, mRNA stability elements, splice sites, leader sequences for enhancing or modifying transport or secretion properties of the protein, or other sequences which alter or improve the function or stability of protein or RNA molecules.

The targeting event may be a simple insertion of the regulatory sequence, placing the gene under the control of the new regulatory sequence, e.g., inserting a new promoter or enhancer or both upstream of a gene. Alternatively, the targeting event may be a simple deletion of a regulatory element, such as the deletion of a tissue-specific negative regulatory element. Alternatively, the targeting event may replace an existing element; for example, a tissue-specific enhancer can be replaced by an enhancer that has broader or different cell-type specificity than the naturally occurring elements. Here, the naturally occurring sequences are deleted and new sequences are added. In all cases, the identification of the targeting event may be facilitated by the use of one or more selectable marker genes that are contiguous with the targeting DNA, allowing for the selection of cells in which the exogenous DNA has integrated into the cell genome. The identification of the targeting event may also be facilitated by the use of one or more marker genes exhibiting the property of negative selection, such that the negatively selectable marker is linked to the exogenous DNA, but configured such that the negatively selectable marker flanks the targeting sequence, and such that a correct homologous recombination event with sequences in the host cell genome does not result in the stable integration of the negatively selectable marker. Markers useful for this purpose include the Herpes Simplex Virus thymidine kinase (TK) gene or the bacterial xanthine-guanine phosphoribosyl-transferase (gpt) gene.

The gene targeting or gene activation techniques which can be used in accordance with this aspect of the invention are more particularly described in U.S. Pat. No. 5,272,071 to Chappel; U.S. Pat. No. 5,578,461 to Sherwin et al.; International Application No. PCT/US92/09627 (WO93/09222) by Selden et al.; and International Application No. PCT/US90/06436 (WO91/06667) by Skoultchi et al., each of which is incorporated by reference herein in its entirety.

4.6 TRANSGENIC ANIMALS

In preferred methods to determine biological functions of the polypeptides of the invention in vivo, one or more genes provided by the invention are either over expressed or inactivated in the germ line of animals using homologous recombination [Capecchi, Science 244:1288-1292 (1989)]. Animals in which the gene is over expressed, under the regulatory control of exogenous or endogenous promoter elements, are known as transgenic animals. Animals in which an endogenous gene has been inactivated by homologous recombination are referred to as “knockout” animals. Knockout animals, preferably non-human mammals, can be prepared as described in U.S. Pat. No. 5,557,032, incorporated herein by reference. Transgenic animals are useful to determine the roles polypeptides of the invention play in biological processes, and preferably in disease states. Transgenic animals are useful as model systems to identify compounds that modulate lipid metabolism. Transgenic animals, preferably non-human mammals, are produced using methods as described in U.S. Pat. No 5,489,743 and PCT Publication No. WO94/28122, incorporated herein by reference.

Transgenic animals can be prepared wherein all or part of a promoter of the polynucleotides of the invention is either activated or inactivated to alter the level of expression of the polypeptides of the invention. Inactivation can be carried out using homologous recombination methods described above. Activation can be achieved by supplementing or even replacing the homologous promoter to provide for increased protein expression. The homologous promoter can be supplemented by insertion of one or more heterologous enhancer elements known to confer promoter activation in a particular tissue.

The polynucleotides of the present invention also make possible the development, through, e.g., homologous recombination or knock out strategies, of animals that fail to express polypeptides of the invention or that express a variant polypeptide. Such animals are useful as models for studying the in vivo activities of polypeptide as well as for studying modulators of the polypeptides of the invention.

In preferred methods to determine biological functions of the polypeptides of the invention in vivo, one or more genes provided by the invention are either over expressed or inactivated in the germ line of animals using homologous recombination [Capecchi, Science 244:1288-1292 (1989)]. Animals in which the gene is over expressed, under the regulatory control of exogenous or endogenous promoter elements, are known as transgenic animals. Animals in which an endogenous gene has been inactivated by homologous recombination are referred to as “knockout” animals. Knockout animals, preferably non-human mammals, can be prepared as described in U.S. Pat. No. 5,557,032, incorporated herein by reference. Transgenic animals are useful to determine the roles polypeptides of the invention play in biological processes, and preferably in disease states. Transgenic animals are useful as model systems to identify compounds that modulate lipid metabolism. Transgenic animals, preferably non-human mammals, are produced using methods as described in U.S. Pat. No 5,489,743 and PCT Publication No. WO94/28122, incorporated herein by reference.

Transgenic animals can be prepared wherein all or part of the polynucleotides of the invention promoter is either activated or inactivated to alter the level of expression of the polypeptides of the invention. Inactivation can be carried out using homologous recombination methods described above. Activation can be achieved by supplementing or even replacing the homologous promoter to provide for increased protein expression. The homologous promoter can be supplemented by insertion of one or more heterologous enhancer elements known to confer promoter activation in a particular tissue.

4.7 USES AND BIOLOGICAL ACTIVITY

The polynucleotides and proteins of the present invention are expected to exhibit one or more of the uses or biological activities (including those associated with assays cited herein) identified herein. Uses or activities described for proteins of the present invention may be provided by administration or use of such proteins or of polynucleotides encoding such proteins (such as, for example, in gene therapies or vectors suitable for introduction of DNA). The mechanism underlying the particular condition or pathology will dictate whether the polypeptides of the invention, the polynucleotides of the invention or modulators (activators or inhibitors) thereof would be beneficial to the subject in need of treatment. Thus, “therapeutic compositions of the invention” include compositions comprising isolated polynucleotides (including recombinant DNA molecules, cloned genes and degenerate variants thereof) or polypeptides of the invention (including full length protein, mature protein and truncations or domains thereof), or compounds and other substances that modulate the overall activity of the target gene products, either at the level of target gene/protein expression or target protein activity. Such modulators include polypeptides, analogs, (variants), including fragments and fusion proteins, antibodies and other binding proteins; chemical compounds that directly or indirectly activate or inhibit the polypeptides of the invention (identified, e.g., via drug screening assays as described herein); antisense polynucleotides and polynucleotides suitable for triple helix formation; and in particular antibodies or other binding partners that specifically recognize one or more epitopes of the polypeptides of the invention.

The polypeptides of the present invention may likewise be involved in cellular activation or in one of the other physiological pathways described herein.

4.7.1 RESEARCH USES AND UTILITIES

The polynucleotides provided by the present invention can be used by the research community for various purposes. The polynucleotides can be used to express recombinant protein for analysis, characterization or therapeutic use; as markers for tissues in which the corresponding protein is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in disease states); as molecular weight markers on gels; as chromosome markers or tags (when labeled) to identify chromosomes or to map related gene positions; to compare with endogenous DNA sequences in patients to identify potential genetic disorders; as probes to hybridize and thus discover novel, related DNA sequences; as a source of information to derive PCR primers for genetic fingerprinting; as a probe to “subtract-out” known sequences in the process of discovering other novel polynucleotides; for selecting and making oligomers for attachment to a “gene chip” or other support, including for examination of expression patterns; to raise anti-protein antibodies using DNA immunization techniques; and as an antigen to raise anti-DNA antibodies or elicit another immune response. Where the polynucleotide encodes a protein which binds or potentially binds to another protein (such as, for example, in a receptor-ligand interaction), the polynucleotide can also be used in interaction trap assays (such as, for example, that described in Gyuris et al., Cell 75:791-803 (1993)) to identify polynucleotides encoding the other protein with which binding occurs or to identify inhibitors of the binding interaction.

The polypeptides provided by the present invention can similarly be used in assays to determine biological activity, including in a panel of multiple proteins for high-throughput screening; to raise antibodies or to elicit another immune response; as a reagent (including the labeled reagent) in assays designed to quantitatively determine levels of the protein (or its receptor) in biological fluids; as markers for tissues in which the corresponding polypeptide is preferentially expressed (either constitutively or at a particular stage of tissue differentiation or development or in a disease state); and, of course, to isolate correlative receptors or ligands. Proteins involved in these binding interactions can also be used to screen for peptide or small molecule inhibitors or agonists of the binding interaction.

Any or all of these research utilities are capable of being developed into reagent grade or kit format for commercialization as research products.

Methods for performing the uses listed above are well known to those skilled in the art. References disclosing such methods include without limitation “Molecular Cloning: A Laboratory Manual”, 2d ed., Cold Spring Harbor Laboratory Press, Sambrook, J., E. F. Fritsch and T. Maniatis eds., 1989, and “Methods in Enzymology: Guide to Molecular Cloning Techniques”, Academic Press, Berger, S. L. and A. R. Kimmel eds., 1987.

4.7.2 NUTRITIONAL USES

Polynucleotides and polypeptides of the present invention can also be used as nutritional sources or supplements. Such uses include without limitation use as a protein or amino acid supplement, use as a carbon source, use as a nitrogen source and use as a source of carbohydrate. In such cases the polypeptide or polynucleotide of the invention can be added to the feed of a particular organism or can be administered as a separate solid or liquid preparation, such as in the form of powder, pills, solutions, suspensions or capsules. In the case of microorganisms, the polypeptide or polynucleotide of the invention can be added to the medium in or on which the microorganism is cultured.

4.7.3 CYTOKINE AND CELL PROLIFERATION/DIFFERENTIATION ACTIVITY

A polypeptide of the present invention may exhibit activity relating to cytokine, cell proliferation (either inducing or inhibiting) or cell differentiation (either inducing or inhibiting) activity or may induce production of other cytokines in certain cell populations. A polynucleotide of the invention can encode a polypeptide exhibiting such attributes. Many protein factors discovered to date, including all known cytokines, have exhibited activity in one or more factor-dependent cell proliferation assays, and hence the assays serve as a convenient confirmation of cytokine activity. The activity of therapeutic compositions of the present invention is evidenced by any one of a number of routine factor dependent cell proliferation assays for cell lines including, without limitation, 32D, DA2, DA1G, T10, B9, B9/11, BaF3, MC9/G, M+(preB M+), 2E8, RB5, DA1, 123, T1165, HT2, CTLL2, TF-1, Mo7e, CMK, HUVEC, and Caco. Therapeutic compositions of the invention can be used in the following:

Assays for T-cell or thymocyte proliferation include without limitation those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Bertagnolli et al., J. Immunol. 145:1706-1712, 1990; Bertagnolli et al., Cellular Immunology 133:327-341, 1991; Bertagnolli, et al., I. Immunol. 149:3778-3783, 1992; Bowman et al., I. Immunol. 152:1756-1761, 1994.

Assays for cytokine production and/or proliferation of spleen cells, lymph node cells or thymocytes include, without limitation, those described in: Polyclonal T cell stimulation, Kruisbeek, A. M. and Shevach, E. M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.12.1-3.12.14, John Wiley and Sons, Toronto. 1994; and Measurement of mouse and human interleukin-γ, Schreiber, R. D. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.8.1-6.8.8, John Wiley and Sons, Toronto. 1994.

Assays for proliferation and differentiation of hematopoietic and lymphopoietic cells include, without limitation, those described in: Measurement of Human and Murine Interleukin 2 and Interleukin 4, Bottomly, K., Davis, L. S. and Lipsky, P. E. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 6.3.1-6.3.12, John Wiley and Sons, Toronto. 1991; deVries et al., J. Exp. Med. 173:1205-1211, 1991; Moreau et al., Nature 336:690-692, 1988; Greenberger et al., Proc. Natl. Acad. Sci. U.S.A. 80:2931-2938, 1983; Measurement of mouse and human interleukin 6—Nordan, R. In Current Protocols in Immunology. J. E. Coligan eds. Vol 1 pp. 6.6.1-6.6.5, John Wiley and Sons, Toronto. 1991; Smith et al., Proc. Natl. Aced. Sci. U.S.A. 83:1857-1861, 1986; Measurement of human Interleukin 11—Bennett, F., Giannotti, J., Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. Coligan eds. Vol 1 pp. 6.15.1 John Wiley and Sons, Toronto. 1991; Measurement of mouse and human Interleukin 9—Ciarletta, A., Giannotti, J., Clark, S. C. and Turner, K. J. In Current Protocols in Immunology. J. E. Coligan eds. Vol 1 pp. 6.13.1, John Wiley and Sons, Toronto. 1991.

Assays for T-cell clone responses to antigens (which will identify, among others, proteins that affect APC-T cell interactions as well as direct T-cell effects by measuring proliferation and cytokine production) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function; Chapter 6, Cytokines and their cellular receptors; Chapter 7, Immunologic studies in Humans); Weinberger et al., Proc. Natl. Acad. Sci. USA 77:6091-6095, 1980; Weinberger et al., Eur. J. Immun. 11:405-411, 1981; Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988.

4.7.4 STEM CELL GROWTH FACTOR ACTIVITY

A polypeptide of the present invention may exhibit stem cell growth factor activity and be involved in the proliferation, differentiation and survival of pluripotent and totipotent stem cells including primordial germ cells, embryonic stem cells, hematopoietic stem cells and/or germ line stem cells. Administration of the polypeptide of the invention to stem cells in vivo or ex vivo is expected to maintain and expand cell populations in a totipotential or pluripotential state which would be useful for re-engineering damaged or diseased tissues, transplantation, manufacture of bio-pharmaceuticals and the development of bio-sensors. The ability to produce large quantities of human cells has important working applications for the production of human proteins which currently must be obtained from non-human sources or donors, implantation of cells to treat diseases such as Parkinson's, Alzheimer's and other neurodegenerative diseases; tissues for grafting such as bone marrow, skin, cartilage, tendons, bone, muscle (including cardiac muscle), blood vessels, cornea, neural cells, gastrointestinal cells and others; and organs for transplantation such as kidney, liver, pancreas (including islet cells), heart and lung.

It is contemplated that multiple different exogenous growth factors and/or cytokines may be administered in combination with the polypeptide of the invention to achieve the desired effect, including any of the growth factors listed herein, other stem cell maintenance factors, and specifically including stem cell factor (SCF), leukemia inhibitory factor (LIF), Flt-3 ligand (Flt-3L), any of the interleukins, recombinant soluble IL-6 receptor fused to IL-6, macrophage inflammatory protein 1-alpha (MIP-1-alpha), G-CSF, GM-CSF, thrombopoietin (TPO), platelet factor 4 (PF-4), platelet-derived growth factor (PDGF), neural growth factors and basic fibroblast growth factor (bFGF).

Since totipotent stem cells can give rise to virtually any mature cell type, expansion of these cells in culture will facilitate the production of large quantities of mature cells. Techniques for culturing stem cells are known in the art and administration of polypeptides of the invention, optionally with other growth factors and/or cytokines, is expected to enhance the survival and proliferation of the stem cell populations. This can be accomplished by direct administration of the polypeptide of the invention to the culture medium. Alternatively, stroma cells transfected with a polynucleotide that encodes for the polypeptide of the invention can be used as a feeder layer for the stem cell populations in culture or in vivo. Stromal support cells for feeder layers may include embryonic bone marrow fibroblasts, bone marrow stromal cells, fetal liver cells, or cultured embryonic fibroblasts (see U.S. Pat. No. 5,690,926).

Stem cells themselves can be transfected with a polynucleotide of the invention to induce autocrine expression of the polypeptide of the invention. This will allow for generation of undifferentiated totipotential/pluripotential stem cell lines that are useful as is or that can then be differentiated into the desired mature cell types. These stable cell lines can also serve as a source of undifferentiated totipotential/pluripotential mRNA to create cDNA libraries and templates for polymerase chain reaction experiments. These studies would allow for the isolation and identification of differentially expressed genes in stem cell populations that regulate stem cell proliferation and/or maintenance.

Expansion and maintenance of totipotent stem cell populations will be useful in the treatment of many pathological conditions. For example, polypeptides of the present invention may be used to manipulate stem cells in culture to give rise to neuroepithelial cells that can be used to augment or replace cells damaged by illness, autoimmune disease, accidental damage or genetic disorders. The polypeptide of the invention may be useful for inducing the proliferation of neural cells and for the regeneration of nerve and brain tissue, i.e. for the treatment of central and peripheral nervous system diseases and neuropathies, as well as mechanical and traumatic disorders which involve degeneration, death or trauma to neural cells or nerve tissue. In addition, the expanded stem cell populations can also be genetically altered for gene therapy purposes and to decrease host rejection of replacement tissues after grafting or implantation.

Expression of the polypeptide of the invention and its effect on stem cells can also be manipulated to achieve controlled differentiation of the stem cells into more differentiated cell types. A broadly applicable method of obtaining pure populations of a specific differentiated cell type from undifferentiated stem cell populations involves the use of a cell-type specific promoter driving a selectable marker. The selectable marker allows only cells of the desired type to survive. For example, stem cells can be induced to differentiate into cardiomyocytes (Wobus et al., Differentiation, 48: 173-182, (1991); Klug et al., J. Clin. Invest., 98(1): 216-224, (1998)) or skeletal muscle cells (Browder, L. W. In: Principles of Tissue Engineering eds . Lanza et al., Academic Press (1997)). Alternatively, directed differentiation of stem cells can be accomplished by culturing the stem cells in the presence of a differentiation factor such as retinoic acid and an antagonist of the polypeptide of the invention which would inhibit the effects of endogenous stem cell factor activity and allow differentiation to proceed.

In vitro cultures of stem cells can be used to determine if the polypeptide of the invention exhibits stem cell growth factor activity. Stem cells are isolated from any one of various cell sources (including hematopoietic stem cells and embryonic stem cells) and cultured on a feeder layer, as described by Thompson et al. Proc. Natl. Acad. Sci, U.S.A., 92: 7844-7848 (1995), in the presence of the polypeptide of the invention alone or in combination with other growth factors or cytokines. The ability of the polypeptide of the invention to induce stem cells proliferation is determined by colony formation on semi-solid support e.g. as described by Bernstein et al., Blood, 77: 2316-2321 (1991).

4.7.5 HEMATOPOIESIS REGULATING ACTIVITY

A polypeptide of the present invention may be involved in regulation of hematopoiesis and, consequently, in the treatment of myeloid or lymphoid cell disorders. Even marginal biological activity in support of colony forming cells or of factor-dependent cell lines indicates involvement in regulating hematopoiesis, e.g. in supporting the growth and proliferation of erythroid progenitor cells alone or in combination with other cytokines, thereby indicating utility, for example, in treating various anemias or for use in conjunction with irradiation/chemotherapy to stimulate the production of erythroid precursors and/or erythroid cells; in supporting the growth and proliferation of myeloid cells such as granulocytes and monocytes/macrophages (i.e., traditional CSF activity) useful, for example, in conjunction with chemotherapy to prevent or treat consequent myelo-suppression; in supporting the growth and proliferation of megakaryocytes and consequently of platelets thereby allowing prevention or treatment of various platelet disorders such as thrombocytopenia, and generally for use in place of or complimentary to platelet transfusions; and/or in supporting the growth and proliferation of hematopoietic stem cells which are capable of maturing to any and all of the above-mentioned hematopoietic cells and therefore find therapeutic utility in various stem cell disorders (such as those usually treated with transplantation, including, without limitation, aplastic anemia and paroxysmal nocturnal hemoglobinuria), as well as in repopulating the stem cell compartment post irradiation/chemotherapy, either in-vivo or ex-vivo (i.e., in conjunction with bone marrow transplantation or with peripheral progenitor cell transplantation (homologous or heterologous)) as normal cells or genetically manipulated for gene therapy.

Therapeutic compositions of the invention can be used in the following:

Suitable assays for proliferation and differentiation of various hematopoietic lines are cited above.

Assays for embryonic stem cell differentiation (which will identify, among others, proteins that influence embryonic differentiation hematopoiesis) include, without limitation, those described in: Johansson et al. Cellular Biology 15:141-151, 1995; Keller et al., Molecular and Cellular Biology 13:473-486, 1993; McClanahan et al., Blood 81:2903-2915, 1993.

Assays for stem cell survival and differentiation (which will identify, among others, proteins that regulate lympho-hematopoiesis) include, without limitation, those described in: Methylcellulose colony forming assays, Freshney, M. G. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 265-268, Wiley-Liss, Inc., New York, N.Y. 1994; Hirayama et al., Proc. Natl. Acad. Sci. USA 89:5907-5911, 1992; Primitive hematopoietic colony forming cells with high proliferative potential, McNiece, I. K. and Briddell, R. A. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 23-39, Wiley-Liss, Inc., New York, N.Y. 1994; Neben et al., Experimental Hematology 22:353-359, 1994; Cobblestone area forming cell assay, Ploemacher, R. E. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 1-21, Wiley-Liss, Inc., New York, N.Y. 1994; Long term bone marrow cultures in the presence of stromal cells, Spooncer, E., Dexter, M. and Allen, T. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 163-179, Wiley-Liss, Inc., New York, N.Y. 1994; Long term culture initiating cell assay, Sutherland, H. J. In Culture of Hematopoietic Cells. R. I. Freshney, et al. eds. Vol pp. 139-162, Wiley-Liss, Inc., New York, N.Y. 1994.

4.7.6 TISSUE GROWTH ACTIVITY

A polypeptide of the present invention also may be involved in bone, cartilage, tendon, ligament and/or nerve tissue growth or regeneration, as well as in wound healing and tissue repair and replacement, and in healing of burns, incisions and ulcers.

A polypeptide of the present invention which induces cartilage and/or bone growth in circumstances where bone is not normally formed, has application in the healing of bone fractures and cartilage damage or defects in humans and other animals. Compositions of a polypeptide, antibody, binding partner, or other modulator of the invention may have prophylactic use in closed as well as open fracture reduction and also in the improved fixation of artificial joints. De novo bone formation induced by an osteogenic agent contributes to the repair of congenital, trauma induced, or oncologic resection induced craniofacial defects, and also is useful in cosmetic plastic surgery.

A polypeptide of this invention may also be involved in attracting bone-forming cells, stimulating growth of bone-forming cells, or inducing differentiation of progenitors of bone-forming cells. Treatment of osteoporosis, osteoarthritis, bone degenerative disorders, or periodontal disease, such as through stimulation of bone and/or cartilage repair or by blocking inflammation or processes of tissue destruction (collagenase activity, osteoclast activity, etc.) mediated by inflammatory processes may also be possible using the composition of the invention.

Another category of tissue regeneration activity that may involve the polypeptide of the present invention is tendon/ligament formation. Induction of tendon/ligament-like tissue or other tissue formation in circumstances where such tissue is not normally formed, has application in the healing of tendon or ligament tears, deformities and other tendon or ligament defects in humans and other animals. Such a preparation employing a tendon/ligament-like tissue inducing protein may have prophylactic use in preventing damage to tendon or ligament tissue, as well as use in the improved fixation of tendon or ligament to bone or other tissues, and in repairing defects to tendon or ligament tissue. De novo tendon/ligament-like tissue formation induced by a composition of the present invention contributes to the repair of congenital, trauma induced, or other tendon or ligament defects of other origin, and is also useful in cosmetic plastic surgery for attachment or repair of tendons or ligaments. The compositions of the present invention may provide environment to attract tendon- or ligament-forming cells, stimulate growth of tendon- or ligament-forming cells, induce differentiation of progenitors of tendon- or ligament-forming cells, or induce growth of tendon/ligament cells or progenitors ex vivo for return in vivo to effect tissue repair. The compositions of the invention may also be useful in the treatment of tendinitis, carpal tunnel syndrome and other tendon or ligament defects. The compositions may also include an appropriate matrix and/or sequestering agent as a carrier as is well known in the art.

The compositions of the present invention may also be useful for proliferation of neural cells and for regeneration of nerve and brain tissue, i.e. for the treatment of central and peripheral nervous system diseases and neuropathies, as well as mechanical and traumatic disorders, which involve degeneration, death or trauma to neural cells or nerve tissue. More specifically, a composition may be used in the treatment of diseases of the peripheral nervous system, such as peripheral nerve injuries, peripheral neuropathy and localized neuropathies, and central nervous system diseases, such as Alzheimer's, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis, and Shy-Drager syndrome. Further conditions which may be treated in accordance with the present invention include mechanical and traumatic disorders, such as spinal cord disorders, head trauma and cerebrovascular diseases such as stroke. Peripheral neuropathies resulting from chemotherapy or other medical therapies may also be treatable using a composition of the invention.

Compositions of the invention may also be useful to promote better or faster closure of non-healing wounds, including without limitation pressure ulcers, ulcers associated with vascular insufficiency, surgical and traumatic wounds, and the like.

Compositions of the present invention may also be involved in the generation or regeneration of other tissues, such as organs (including, for example, pancreas, liver, intestine, kidney, skin, endothelium), muscle (smooth, skeletal or cardiac) and vascular (including vascular endothelium) tissue, or for promoting the growth of cells comprising such tissues. Part of the desired effects may be by inhibition or modulation of fibrotic scarring may allow normal tissue to regenerate. A polypeptide of the present invention may also exhibit angiogenic activity.

A composition of the present invention may also be useful for gut protection or regeneration and treatment of lung or liver fibrosis, reperfusion injury in various tissues, and conditions resulting from systemic cytokine damage.

A composition of the present invention may also be useful for promoting or inhibiting differentiation of tissues described above from precursor tissues or cells; or for inhibiting the growth of tissues described above.

Therapeutic compositions of the invention can be used in the following:

Assays for tissue generation activity include, without limitation, those described in: International Patent Publication No. WO95/16035 (bone, cartilage, tendon); International Patent Publication No. WO95/05846 (nerve, neuronal); International Patent Publication No. WO91/07491 (skin, endothelium).

Assays for wound healing activity include, without limitation, those described in: Winter, Epidermal Wound Healing, pps. 71-112 (Maibach, H. I. and Rovee, D. T., eds.), Year Book Medical Publishers, Inc., Chicago, as modified by Eaglstein and Mertz, J. Invest. Dermatol 71:382-84 (1978).

4.7.7 IMMUNE STIMULATING OR SUPPRESSING ACTIVITY

A polypeptide of the present invention may also exhibit immune stimulating or immune suppressing activity, including without limitation the activities for which assays are described herein. A polynucleotide of the invention can encode a polypeptide exhibiting such activities. A protein may be useful in the treatment of various immune deficiencies and disorders (including severe combined immunodeficiency (SCID)), e.g., in regulating (up or down) growth and proliferation of T and/or B lymphocytes, as well as effecting the cytolytic activity of NK cells and other cell populations. These immune deficiencies may be genetic or be caused by viral (e.g., HIV) as well as bacterial or fungal infections, or may result from autoimmune disorders. More specifically, infectious diseases causes by viral, bacterial, fungal or other infection may be treatable using a protein of the present invention, including infections by HIV, hepatitis viruses, herpes viruses, mycobacteria, Leishmania spp., malaria spp. and various fungal infections such as candidiasis. Of course, in this regard, proteins of the present invention may also be useful where a boost to the immune system generally may be desirable, i.e., in the treatment of cancer.

Autoimmune disorders which may be treated using a protein of the present invention include, for example, connective tissue disease, multiple sclerosis, systemic lupus erythematosus, rheumatoid arthritis, autoimmune pulmonary inflammation, Guillain-Barre syndrome, autoimmune thyroiditis, insulin dependent diabetes mellitis, myasthenia gravis, graft-versus-host disease and autoimmune inflammatory eye disease. Such a protein (or antagonists thereof, including antibodies) of the present invention may also to be useful in the treatment of allergic reactions and conditions (e.g., anaphylaxis, serum sickness, drug reactions, food allergies, insect venom allergies, mastocytosis, allergic rhinitis, hypersensitivity pneumonitis, urticaria, angioedema, eczema, atopic dermatitis, allergic contact dermatitis, erythema multiforme, Stevens-Johnson syndrome, allergic conjunctivitis, atopic keratoconjunctivitis, venereal keratoconjunctivitis, giant papillary conjunctivitis and contact allergies), such as asthma (particularly allergic asthma) or other respiratory problems. Other conditions, in which immune suppression is desired (including, for example, organ transplantation), may also be treatable using a protein (or antagonists thereof) of the present invention. The therapeutic effects of the polypeptides or antagonists thereof on allergic reactions can be evaluated by in vivo animals models such as the cumulative contact enhancement test (Lastbom et al., Toxicology 125: 59-66,1998), skin prick test (Hoffmann et al., Allergy 54: 446-54, 1999), guinea pig skin sensitization test (Vohr et al., Arch. Toxocol. 73: 501-9), and murine local lymph node assay (Kimber et al., J. Toxicol. Environ. Health 53: 563-79).

Using the proteins of the invention it may also be possible to modulate immune responses, in a number of ways. Down regulation may be in the form of inhibiting or blocking an immune response already in progress or may involve preventing the induction of an immune response. The functions of activated T cells may be inhibited by suppressing T cell responses or by inducing specific tolerance in T cells, or both. Immunosuppression of T cell responses is generally an active, non-antigen-specific, process which requires continuous exposure of the T cells to the suppressive agent. Tolerance, which involves inducing non-responsiveness or anergy in T cells, is distinguishable from immunosuppression in that it is generally antigen-specific and persists after exposure to the tolerizing agent has ceased. Operationally, tolerance can be demonstrated by the lack of a T cell response upon reexposure to specific antigen in the absence of the tolerizing agent.

Down regulating or preventing one or more antigen functions (including without limitation B lymphocyte antigen functions (such as, for example, B7)), e.g., preventing high level lymphokine synthesis by activated T cells, will be useful in situations of tissue, skin and organ transplantation and in graft-versus-host disease (GVHD). For example, blockage of T cell function should result in reduced tissue destruction in tissue transplantation. Typically, in tissue transplants, rejection of the transplant is initiated through its recognition as foreign by T cells, followed by an immune reaction that destroys the transplant. The administration of a therapeutic composition of the invention may prevent cytokine synthesis by immune cells, such as T cells, and thus acts as an immunosuppressant. Moreover, a lack of costimulation may also be sufficient to anergize the T cells, thereby inducing tolerance in a subject. Induction of long-term tolerance by B lymphocyte antigen-blocking reagents may avoid the necessity of repeated administration of these blocking reagents. To achieve sufficient immunosuppression or tolerance in a subject, it may also be necessary to block the function of a combination of B lymphocyte antigens.

The efficacy of particular therapeutic compositions in preventing organ transplant rejection or GVHD can be assessed using animal models that are predictive of efficacy in humans. Examples of appropriate systems which can be used include allogeneic cardiac grafts in rats and xenogeneic pancreatic islet cell grafts in mice, both of which have been used to examine the immunosuppressive effects of CTLA4Ig fusion proteins in vivo as described in Lenschow et al., Science 257:789-792 (1992) and Turka et al., Proc. Natl. Acad. Sci USA, 89:11102-11105 (1992). In addition, murine models of GVHD (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp.846-847) can be used to determine the effect of therapeutic compositions of the invention on the development of that disease.

Blocking antigen function may also be therapeutically useful for treating autoimmune diseases. Many autoimmune disorders are the result of inappropriate activation of T cells that are reactive against self tissue and which promote the production of cytokines and autoantibodies involved in the pathology of the diseases. Preventing the activation of autoreactive T cells may reduce or eliminate disease symptoms. Administration of reagents which block stimulation of T cells can be used to inhibit T cell activation and prevent production of autoantibodies or T cell-derived cytokines which may be involved in the disease process. Additionally, blocking reagents may induce antigen-specific tolerance of autoreactive T cells which could lead to long-term relief from the disease. The efficacy of blocking reagents in preventing or alleviating autoimmune disorders can be determined using a number of well-characterized animal models of human autoimmune diseases. Examples include murine experimental autoimmune encephalitis, systemic lupus erythmatosis in MRL/lpr/lpr mice or NZB hybrid mice, murine autoimmune collagen arthritis, diabetes mellitus in NOD mice and BB rats, and murine experimental myasthenia gravis (see Paul ed., Fundamental Immunology, Raven Press, New York, 1989, pp. 840-856).

Upregulation of an antigen function (e.g., a B lymphocyte antigen function), as a means of up regulating immune responses, may also be useful in therapy. Upregulation of immune responses may be in the form of enhancing an existing immune response or eliciting an initial immune response. For example, enhancing an immune response may be useful in cases of viral infection, including systemic viral diseases such as influenza, the common cold, and encephalitis.

Alternatively, anti-viral immune responses may be enhanced in an infected patient by removing T cells from the patient, costimulating the T cells in vitro with viral antigen-pulsed APCs either expressing a peptide of the present invention or together with a stimulatory form of a soluble peptide of the present invention and reintroducing the in vitro activated T cells into the patient. Another method of enhancing anti-viral immune responses would be to isolate infected cells from a patient, transfect them with a nucleic acid encoding a protein of the present invention as described herein such that the cells express all or a portion of the protein on their surface, and reintroduce the transfected cells into the patient. The infected cells would now be capable of delivering a costimulatory signal to, and thereby activate, T cells in vivo.

A polypeptide of the present invention may provide the necessary stimulation signal to T cells to induce a T cell mediated immune response against the transfected tumor cells. In addition, tumor cells which lack MHC class I or MHC class II molecules, or which fail to reexpress sufficient mounts of MHC class I or MHC class II molecules, can be transfected with nucleic acid encoding all or a portion of (e.g., a cytoplasmic-domain truncated portion) of an MHC class I alpha chain protein and p2 microglobulin protein or an MHC class II alpha chain protein and an MHC class 11 beta chain protein to thereby express MHC class I or MHC class II proteins on the cell surface. Expression of the appropriate class I or class II MHC in conjunction with a peptide having the activity of a B lymphocyte antigen (e.g., B7-1, B7-2, B7-3) induces a T cell mediated immune response against the transfected tumor cell. Optionally, a gene encoding an antisense construct which blocks expression of an MHC class II associated protein, such as the invariant chain, can also be cotransfected with a DNA encoding a peptide having the activity of a B lymphocyte antigen to promote presentation of tumor associated antigens and induce tumor specific immunity. Thus, the induction of a T cell mediated immune response in a human subject may be sufficient to overcome tumor-specific tolerance in the subject.

The activity of a protein of the invention may, among other means, be measured by the following methods:

Suitable assays for thymocyte or splenocyte cytotoxicity include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Herrmann et al., Proc. Natl. Acad. Sci. USA 78:2488-2492, 1981; Herrmann et al., J. Immunol. 128:1968-1974, 1982; Handa et al., J. Immunol. 135:1564-1572, 1985; Takai et al., I. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Bowman et al., J. Virology 61:1992-1998; Bertagnolli et al., Cellular Immunology 133:327-341, 1991; Brown et al., J. Immunol. 153:3079-3092, 1994.

Assays for T-cell-dependent immunoglobulin responses and isotype switching (which will identify, among others, proteins that modulate T-cell dependent antibody responses and that affect Th1/Th2 profiles) include, without limitation, those described in: Maliszewski, J. Immunol. 144:3028-3033, 1990; and Assays for B cell function: In vitro antibody production, Mond, J. J. and Brunswick, M. In Current Protocols in Immunology. J. E. e.a. Coligan eds. Vol 1 pp. 3.8.1-3.8.16, John Wiley and Sons, Toronto. 1994.

Mixed lymphocyte reaction (MLR) assays (which will identify, among others, proteins that generate predominantly Th1 and CTL responses) include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 3, In Vitro assays for Mouse Lymphocyte Function 3.1-3.19; Chapter 7, Immunologic studies in Humans); Takai et al., J. Immunol. 137:3494-3500, 1986; Takai et al., J. Immunol. 140:508-512, 1988; Bertagnolli et al., J. Immunol. 149:3778-3783, 1992.

Dendritic cell-dependent assays (which will identify, among others, proteins expressed by dendritic cells that activate naive T-cells) include, without limitation, those described in: Guery et al., J. Immunol. 134:536-544, 1995; Inaba et al., Journal of Experimental Medicine 173:549-559, 1991; Macatonia et al., Journal of Immunology 154:5071-5079, 1995; Porgador et al., Journal of Experimental Medicine 182:255-260, 1995; Nair et al., Journal of Virology 67:4062-4069, 1993; Huang et al., Science 264:961-965, 1994; Macatonia et al., Journal of Experimental Medicine 169:1255-1264, 1989; Bhardwaj et al., Journal of Clinical Investigation 94:797-807, 1994; and Inaba et al., Journal of Experimental Medicine 172:631-640, 1990.

Assays for lymphocyte survival/apoptosis (which will identify, among others, proteins that prevent apoptosis after superantigen induction and proteins that regulate lymphocyte homeostasis) include, without limitation, those described in: Darzynkiewicz et al., Cytometry 13:795-808, 1992; Gorczyca et al., Leukemia 7:659-670, 1993; Gorczyca et al., Cancer Research 53:1945-1951, 1993; Itoh et al., Cell 66:233-243, 1991; Zacharchuk, Journal of Immunology 145:4037-4045, 1990; Zamai et al., Cytometry 14:891-897, 1993; Gorczyca et al., International Journal of Oncology 1:639-648, 1992.

Assays for proteins that influence early steps of T-cell commitment and development include, without limitation, those described in: Antica et al., Blood 84:111-117, 1994; Fine et al., Cellular Immunology 155:111-122, 1994; Galy et al., Blood 85:2770-2778, 1995; Toki et al., Proc. Nat. Acad Sci. USA 88:7548-7551, 1991.

4.7.8 ACTIVIN/INHIBIN ACTIVITY

A polypeptide of the present invention may also exhibit activin- or inhibin-related activities. A polynucleotide of the invention may encode a polypeptide exhibiting such characteristics. Inhibins are characterized by their ability to inhibit the release of follicle stimulating hormone (FSH), while activins and are characterized by their ability to stimulate the release of follicle stimulating hormone (FSH). Thus, a polypeptide of the present invention, alone or in heterodimers with a member of the inhibin family, may be useful as a contraceptive based on the ability of inhibins to decrease fertility in female mammals and decrease spermatogenesis in male mammals. Administration of sufficient amounts of other inhibins can induce infertility in these mammals. Alternatively, the polypeptide of the invention, as a homodimer or as a heterodimer with other protein subunits of the inhibin group, may be useful as a fertility inducing therapeutic, based upon the ability of activin molecules in stimulating FSH release from cells of the anterior pituitary. See, for example, U.S. Pat. No. 4,798,885. A polypeptide of the invention may also be useful for advancement of the onset of fertility in sexually immature mammals, so as to increase the lifetime reproductive performance of domestic animals such as, but not limited to, cows, sheep and pigs.

The activity of a polypeptide of the invention may, among other means, be measured by the following methods.

Assays for activin/inhibin activity include, without limitation, those described in: Vale et al., Endocrinology 91:562-572, 1972; Ling et al., Nature 321:779-782, 1986; Vale et al., Nature 321:776-779, 1986; Mason et al., Nature 318:659-663, 1985; Forage et al., Proc. Natl. Acad. Sci. USA 83:3091-3095, 1986.

4.7.9 CHEMOTACTIC/CHEMOKINETIC ACTIVITY

A polypeptide of the present invention may be involved in chemotactic or chemokinetic activity for mammalian cells, including, for example, monocytes, fibroblasts, neutrophils, T-cells, mast cells, eosinophils, epithelial and/or endothelial cells. A polynucleotide of the invention can encode a polypeptide exhibiting such attributes. Chemotactic and chemokinetic receptor activation can be used to mobilize or attract a desired cell population to a desired site of action. Chemotactic or chemokinetic compositions (e.g. proteins, antibodies, binding partners, or modulators of the invention) provide particular advantages in treatment of wounds and other trauma to tissues, as well as in treatment of localized infections. For example, attraction of lymphocytes, monocytes or neutrophils to tumors or sites of infection may result in improved immune responses against the tumor or infecting agent.

A protein or peptide has chemotactic activity for a particular cell population if it can stimulate, directly or indirectly, the directed orientation or movement of such cell population. Preferably, the protein or peptide has the ability to directly stimulate directed movement of cells. Whether a particular protein has chemotactic activity for a population of cells can be readily determined by employing such protein or peptide in any known assay for cell chemotaxis.

Therapeutic compositions of the invention can be used in the following:

Assays for chemotactic activity (which will identify proteins that induce or prevent chemotaxis) consist of assays that measure the ability of a protein to induce the migration of cells across a membrane as well as the ability of a protein to induce the adhesion of one cell population to another cell population. Suitable assays for movement and adhesion include, without limitation, those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Marguiles, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley-Interscience (Chapter 6.12, Measurement of alpha and beta Chemokines 6.12.1-6.12.28; Taub et al. J. Clin. Invest. 95:1370-1376, 1995; Lind et al. APMIS 103:140-146, 1995; Muller et al Eur. J. Immunol. 25:1744-1748; Gruber et al. J. of Immunol. 152:5860-5867, 1994; Johnston et al. J. of Immunol. 153:1762-1768, 1994.

4.7.10 HEMOSTATIC AND THROMBOLYTIC ACTIVITY

A polypeptide of the invention may also be involved in hemostatis or thrombolysis or thrombosis. A polynucleotide of the invention can encode a polypeptide exhibiting such attributes. Compositions may be useful in treatment of various coagulation disorders (including hereditary disorders, such as hemophilias) or to enhance coagulation and other hemostatic events in treating wounds resulting from trauma, surgery or other causes. A composition of the invention may also be useful for dissolving or inhibiting formation of thromboses and for treatment and prevention of conditions resulting therefrom (such as, for example, infarction of cardiac and central nervous system vessels (e.g., stroke).

Therapeutic compositions of the invention can be used in the following:

Assay for hemostatic and thrombolytic activity include, without limitation, those described in: Linet et al., J. Clin. Pharmacol. 26:131-140, 1986; Burdick et al., Thrombosis Res. 45:413-419, 1987; Humphrey et al., Fibrinolysis 5:71-79 (1991); Schaub, Prostaglandins 35:467-474, 1988.

4.7.11 CANCER DIAGNOSIS AND THERAPY

Polypeptides of the invention may be involved in cancer cell generation, proliferation or metastasis. Detection of the presence or amount of polynucleotides or polypeptides of the invention may be useful for the diagnosis and/or prognosis of one or more types of cancer. For example, the presence or increased expression of a polynucleotide/polypeptide of the invention may indicate a hereditary risk of cancer, a precancerous condition, or an ongoing malignancy. Conversely, a defect in the gene or absence of the polypeptide may be associated with a cancer condition. Identification of single nucleotide polymorphisms associated with cancer or a predisposition to cancer may also be useful for diagnosis or prognosis.

Cancer treatments promote tumor regression by inhibiting tumor cell proliferation, inhibiting angiogenesis (growth of new blood vessels that is necessary to support tumor growth) and/or prohibiting metastasis by reducing tumor cell motility or invasiveness. Therapeutic compositions of the invention may be effective in adult and pediatric oncology including in solid phase tumors/malignancies, locally advanced tumors, human soft tissue sarcomas, metastatic cancer, including lymphatic metastases, blood cell malignancies including multiple myeloma, acute and chronic leukemias, and lymphomas, head and neck cancers including mouth cancer, larynx cancer and thyroid cancer, lung cancers including small cell carcinoma and non-small cell cancers, breast cancers including small cell carcinoma and ductal carcinoma, gastrointestinal cancers including esophageal cancer, stomach cancer, colon cancer, colorectal cancer and polyps associated with colorectal neoplasia, pancreatic cancers, liver cancer, urologic cancers including bladder cancer and prostate cancer, malignancies of the female genital tract including ovarian carcinoma, uterine (including endometrial) cancers, and solid tumor in the ovarian follicle, kidney cancers including renal cell carcinoma, brain cancers including intrinsic brain tumors, neuroblastoma, astrocytic brain tumors, gliomas, metastatic tumor cell invasion in the central nervous system, bone cancers including osteomas, skin cancers including malignant melanoma, tumor progression of human skin keratinocytes, squamous cell carcinoma, basal cell carcinoma, hemangiopericytoma and Karposi's sarcoma.

Polypeptides, polynucleotides, or modulators of polypeptides of the invention (including inhibitors and stimulators of the biological activity of the polypeptide of the invention) may be administered to treat cancer. Therapeutic compositions can be administered in therapeutically effective dosages alone or in combination with adjuvant cancer therapy such as surgery, chemotherapy, radiotherapy, thermotherapy, and laser therapy, and may provide a beneficial effect, e.g. reducing tumor size, slowing rate of tumor growth, inhibiting metastasis, or otherwise improving overall clinical condition, without necessarily eradicating the cancer.

The composition can also be administered in therapeutically effective amounts as a portion of an anti-cancer cocktail. An anti-cancer cocktail is a mixture of the polypeptide or modulator of the invention with one or more anti-cancer drugs in addition to a pharmaceutically acceptable carrier for delivery. The use of anti-cancer cocktails as a cancer treatment is routine. Anti-cancer drugs that are well known in the art and can be used as a treatment in combination with the polypeptide or modulator of the invention include: Actinomycin D, Aminoglutethimide, Asparaginase, Bleomycin, Busulfan, Carboplatin, Carmustine, Chlorambucil, Cisplatin (cis-DDP), Cyclophosphamide, Cytarabine HCl (Cytosine arabinoside), Dacarbazine, Dactinomycin, Daunorubicin HCl, Doxorubicin HCl, Estramustine phosphate sodium, Etoposide (V16-213), Floxuridine, 5-Fluorouracil (5-Fu), Flutamide, Hydroxyurea (hydroxycarbamide), Ifosfamide, Interferon Alpha-2a, Interferon Alpha-2b, Leuprolide acetate (LHRH-releasing factor analog), Lomustine, Mechlorethamine HCl (nitrogen mustard), Melphalan, Mercaptopurine, Mesna, Methotrexate (MTX), Mitomycin, Mitoxantrone HCl, Octreotide, Plicamycin, Procarbazine HCl, Streptozocin, Tamoxifen citrate, Thioguanine, Thiotepa, Vinblastine sulfate, Vincristine sulfate, Amsacrine, Azacitidine, Hexamethylmelamine, Interleukin-2, Mitoguazone, Pentostatin, Semustine, Teniposide, and Vindesine sulfate.

In addition, therapeutic compositions of the invention may be used for prophylactic treatment of cancer. There are hereditary conditions and/or environmental situations (e.g. exposure to carcinogens) known in the art that predispose an individual to developing cancers. Under these circumstances, it may be beneficial to treat these individuals with therapeutically effective doses of the polypeptide of the invention to reduce the risk of developing cancers.

In vitro models can be used to determine the effective doses of the polypeptide of the invention as a potential cancer treatment. These in vitro models include proliferation assays of cultured tumor cells, growth of cultured tumor cells in soft agar (see Freshney, (1987) Culture of Animal Cells: A Manual of Basic Technique, Wily-Liss, New York, N.Y. Ch 18 and Ch 21), tumor systems in nude mice as described in Giovanella et al., J. Natl. Can. Inst., 52: 921-30 (1974), mobility and invasive potential of tumor cells in Boyden Chamber assays as described in Pilkington et al., Anticancer Res., 17: 4107-9 (1997), and angiogenesis assays such as induction of vascularization of the chick chorioallantoic membrane or induction of vascular endothelial cell migration as described in Ribatta et al., Intl. J. Dev. Biol., 40: 1189-97 (1999) and Li et al., Clin. Exp. Metastasis, 17:423-9 (1999), respectively. Suitable tumor cells lines are available, e.g. from American Type Tissue Culture Collection catalogs.

4.7.12 RECEPTOR/LIGAND ACTIVITY

A polypeptide of the present invention may also demonstrate activity as receptor, receptor ligand or inhibitor or agonist of receptor/ligand interactions. A polynucleotide of the invention can encode a polypeptide exhibiting such characteristics. Examples of such receptors and ligands include, without limitation, cytokine receptors and their ligands, receptor kinases and their ligands, receptor phosphatases and their ligands, receptors involved in cell-cell interactions and their ligands (including without limitation, cellular adhesion molecules (such as selectins, integrins and their ligands) and receptor/ligand pairs involved in antigen presentation, antigen recognition and development of cellular and humoral immune responses. Receptors and ligands are also useful for screening of potential peptide or small molecule inhibitors of the relevant receptor/ligand interaction. A protein of the present invention (including, without limitation, fragments of receptors and ligands) may themselves be useful as inhibitors of receptor/ligand interactions.

The activity of a polypeptide of the invention may, among other means, be measured by the following methods:

Suitable assays for receptor-ligand activity include without limitation those described in: Current Protocols in Immunology, Ed by J. E. Coligan, A. M. Kruisbeek, D. H. Margulies, E. M. Shevach, W. Strober, Pub. Greene Publishing Associates and Wiley- Interscience (Chapter 7.28, Measurement of Cellular Adhesion under static conditions 7.28.1-7.28.22), Takai et al., Proc. Nati. Acad. Sci. USA 84:6864-6868, 1987; Bierer et al., J. Exp. Med. 168:1145-1156, 1988; Rosenstein et al., J. Exp. Med. 169:149-160 1989; Stoltenborg et al., J. Immunol. Methods 175:59-68, 1994; Stitt et al., Cell 80:661-670, 1995.

By way of example, the polypeptides of the invention may be used as a receptor for a ligand(s) thereby transmitting the biological activity of that ligand(s). Ligands may be identified through binding assays, affinity chromatography, dihybrid screening assays, BIAcore assays, gel overlay assays, or other methods known in the art.

Studies characterizing drugs or proteins as agonist or antagonist or partial agonists or a partial antagonist require the use of other proteins as competing ligands. The polypeptides of the present invention or ligand(s) thereof may be labeled by being coupled to radioisotopes, colorimetric molecules or a toxin molecules by conventional methods. (“Guide to Protein Purification” Murray P. Deutscher (ed) Methods in Enzymology Vol. 182 (1990) Academic Press, Inc. San Diego). Examples of radioisotopes include, but are not limited to, tritium and carbon-14. Examples of colorimetric molecules include, but are not limited to, fluorescent molecules such as fluorescamine, or rhodamine or other colorimetric molecules. Examples of toxins include, but are not limited, to ricin.

4.7.13 DRUG SCREENING

This invention is particularly useful for screening chemical compounds by using the novel polypeptides or binding fragments thereof in any of a variety of drug screening techniques. The polypeptides or fragments employed in such a test may either be free in solution,. affixed to a solid support, borne on a cell surface or located intracellularly. One method of drug screening utilizes eukaryotic or prokaryotic host cells which are stably transformed with recombinant nucleic acids expressing the polypeptide or a fragment thereof. Drugs are screened against such transformed cells in competitive binding assays. Such cells, either in viable or fixed form, can be used for standard binding assays. One may measure, for example, the formation of complexes between polypeptides of the invention or fragments and the agent being tested or examine the diminution in complex formation between the novel polypeptides and an appropriate cell line, which are well known in the art.

Sources for test compounds that may be screened for ability to bind to or modulate (i.e., increase or decrease) the activity of polypeptides of the invention include (1) inorganic and organic chemical libraries, (2) natural product libraries, and (3) combinatorial libraries comprised of either random or mimetic peptides, oligonucleotides or organic molecules.

Chemical libraries may be readily synthesized or purchased from a number of commercial sources, and may include structural analogs of known compounds or compounds that are identified as “hits” or “leads” via natural product screening.

The sources of natural product libraries are microorganisms (including bacteria and fungi), animals, plants or other vegetation, or marine organisms, and libraries of mixtures for screening may be created by: (1) fermentation and extraction of broths from soil, plant or marine microorganisms or (2) extraction of the organisms themselves. Natural product libraries include polyketides, non-ribosomal peptides, and (non-naturally occurring) variants thereof. For a review, see Science 282:63-68 (1998).

Combinatorial libraries are composed of large numbers of peptides, oligonucleotides or organic compounds and can be readily prepared by traditional automated synthesis methods, PCR, cloning or proprietary synthetic methods. Of particular interest are peptide and oligonucleotide combinatorial libraries. Still other libraries of interest include peptide, protein, peptidomimetic, multiparallel synthetic collection, recombinatorial, and polypeptide libraries. For a review of combinatorial chemistry and libraries created therefrom, see Myers, Curr. Opin. Biotechnol . 8:701-707 (1997). For reviews and examples of peptidomimetic libraries, see Al-Obeidi et al., Mol. Biotechnol , 9(3):205-23 (1998); Hruby et al., Curr Opin Chem Biol , 1(1): 114-19 (1997); Dorner et al., Bioorg Med Chem , 4(5):709-15 (1996) (alkylated dipeptides).

Identification of modulators through use of the various libraries described herein permits modification of the candidate “hit” (or “lead”) to optimize the capacity of the “hit” to bind a polypeptide of the invention. The molecules identified in the binding assay are then tested for antagonist or agonist activity in in vivo tissue culture or animal models that are well known in the art. In brief, the molecules are titrated into a plurality of cell cultures or animals and then tested for either cell/animal death or prolonged survival of the animal/cells.

The binding molecules thus identified may be complexed with toxins, e.g., ricin or cholera, or with other compounds that are toxic to cells such as radioisotopes. The toxin-binding molecule complex is then targeted to a tumor or other cell by the specificity of the binding molecule for a polypeptide of the invention. Alternatively, the binding molecules may be complexed with imaging agents for targeting and imaging purposes.

4.7.14 ASSAY FOR RECEPTOR ACTIVITY

The invention also provides methods to detect specific binding of a polypeptide e.g. a ligand or a receptor. The art provides numerous assays particularly useful for identifying previously unknown binding partners for receptor polypeptides of the invention. For example, expression cloning using mammalian or bacterial cells, or dihybrid screening assays can be used to identify polynucleotides encoding binding partners. As another example, affinity chromatography with the appropriate immobilized polypeptide of the invention can be used to isolate polypeptides that recognize and bind polypeptides of the invention. There are a number of different libraries used for the identification of compounds, and in particular small molecules, that modulate (i.e., increase or decrease) biological activity of a polypeptide of the invention. Ligands for receptor polypeptides of the invention can also be identified by adding exogenous ligands, or cocktails of ligands to two cells populations that are genetically identical except for the expression of the receptor of the invention: one cell population expresses the receptor of the invention whereas the other does not. The response of the two cell populations to the addition of ligands(s) are then compared. Alternatively, an expression library can be co-expressed with the polypeptide of the invention in cells and assayed for an autocrine response to identify potential ligand(s). As still another example, BlAcore assays, gel overlay assays, or other methods known in the art can be used to identify binding partner polypeptides, including, (1) organic and inorganic chemical libraries, (2) natural product libraries, and (3) combinatorial libraries comprised of random peptides, oligonucleotides or organic molecules.

The role of downstream intracellular signaling molecules in the signaling cascade of the polypeptide of the invention can be determined. For example, a chimeric protein in which the cytoplasmic domain of the polypeptide of the invention is fused to the extracellular portion of a protein, whose ligand has been identified, is produced in a host cell. The cell is then incubated with the ligand specific for the extracellular portion of the chimeric protein, thereby activating the chimeric receptor. Known downstream proteins involved in intracellular signaling can then be assayed for expected modifications i.e. phosphorylation. Other methods known to those in the art can also be used to identify signaling molecules involved in receptor activity.

4.7.15 ANTI-INFLAMMATORY ACTIVITY

Compositions of the present invention may also exhibit anti-inflammatory activity. The anti-inflammatory activity may be achieved by providing a stimulus to cells involved in the inflammatory response, by inhibiting or promoting cell-cell interactions (such as, for example, cell adhesion), by inhibiting or promoting chemotaxis of cells involved in the inflammatory process, inhibiting or promoting cell extravasation, or by stimulating or suppressing production of other factors which more directly inhibit or promote an inflammatory response. Compositions with such activities can be used to treat inflammatory conditions including chronic or acute conditions), including without limitation intimation associated with infection (such as septic shock, sepsis or systemic inflammatory response syndrome (SIRS)), ischemia-reperfusion injury, endotoxin lethality, arthritis, complement-mediated hyperacute rejection, nephritis, cytokine or chemokine-induced lung injury, inflammatory bowel disease, Crohn's disease or resulting from over production of cytokines such as TNF or IL-1. Compositions of the invention may also be useful to treat anaphylaxis and hypersensitivity to an antigenic substance or material. Compositions of this invention may be utilized to prevent or treat conditions such as, but not limited to, sepsis, acute pancreatitis, endotoxin shock, cytokine induced shock, rheumatoid arthritis, chronic inflammatory arthritis, pancreatic cell damage from diabetes mellitus type 1, graft versus host disease, inflammatory bowel disease, inflamation associated with pulmonary disease, other autoimmune disease or inflammatory disease, an antiproliferative agent such as for acute or chronic mylegenous leukemia or in the prevention of premature labor secondary to intrauterine infections.

4.7.16 LEUKEMIAS

Leukemias and related disorders may be treated or prevented by administration of a therapeutic that promotes or inhibits function of the polynucleotides and/or polypeptides of the invention. Such leukemias and related disorders include but are not limited to acute leukemia, acute lymphocytic leukemia, acute myelocytic leukemia, myeloblastic, promyelocytic, myelomonocytic, monocytic, erythroleukemia, chronic leukemia, chronic myelocytic (granulocytic) leukemia and chronic lymphocytic leukemia (for a review of such disorders, see Fishman et al., 1985, Medicine, 2d Ed., J.B. Lippincott Co., Philadelphia).

4.7.17 NERVOUS SYSTEM DISORDERS

Nervous system disorders, involving cell types which can be tested for efficacy of intervention with compounds that modulate the activity of the polynucleotides and/or polypeptides of the invention, and which can be treated upon thus observing an indication of therapeutic utility, include but are not limited to nervous system injuries, and diseases or disorders which result in either a disconnection of axons, a diminution or degeneration of neurons, or demyelination. Nervous system lesions which may be treated in a patient (including human and non-human mammalian patients) according to the invention include but are not limited to the following lesions of either the central (including spinal cord, brain) or peripheral nervous systems:

(i) traumatic lesions, including lesions caused by physical injury or associated with surgery, for example, lesions which sever a portion of the nervous system, or compression injuries;

(ii) ischemic lesions, in which a lack of oxygen in a portion of the nervous system results in neuronal injury or death, including cerebral infarction or ischemia, or spinal cord infarction or ischemia;

(iii) infectious lesions, in which a portion of the nervous system is destroyed or injured as a result of infection, for example, by an abscess or associated with infection by human immunodeficiency virus, herpes zoster, or herpes simplex virus or with Lyme disease, tuberculosis, syphilis;

(iv) degenerative lesions, in which a portion of the nervous system is destroyed or injured as a result of a degenerative process including but not limited to degeneration associated with Parkinson's disease, Alzheimer's disease, Huntington's chorea, or amyotrophic lateral sclerosis;

(v) lesions associated with nutritional diseases or disorders, in which a portion of the nervous system is destroyed or injured by a nutritional disorder or disorder of metabolism including but not limited to, vitamin B12 deficiency, folic acid deficiency, Wernicke disease, tobacco-alcohol amblyopia, Marchiafava-Bignami disease (primary degeneration of the corpus callosum), and alcoholic cerebellar degeneration;

(vi) neurological lesions associated with systemic diseases including but not limited to diabetes (diabetic neuropathy, Bell's palsy), systemic lupus erythematosus, carcinoma, or sarcoidosis;

(vii) lesions caused by toxic substances including alcohol, lead, or particular neurotoxins; and

(viii) demyelinated lesions in which a portion of the nervous system is destroyed or injured by a demyelinating disease including but not limited to multiple sclerosis, human immunodeficiency virus-associated myelopathy, transverse myelopathy or various etiologies, progressive multifocal leukoencephalopathy, and central pontine myelinolysis.

Therapeutics which are useful according to the invention for treatment of a nervous system disorder may be selected by testing for biological activity in promoting the survival or differentiation of neurons. For example, and not by way of limitation, therapeutics which elicit any of the following effects may be useful according to the invention:

(i) increased survival time of neurons in culture;

(ii) increased sprouting of neurons in culture or in vivo;

(iii) increased production of a neuron-associated molecule in culture or in vivo, e.g., choline acetyltransferase or acetylcholinesterase with respect to motor neurons; or

(iv) decreased symptoms of neuron dysfunction in vivo.

Such effects may be measured by any method known in the art. In preferred, non-limiting embodiments, increased survival of neurons may be measured by the method set forth in Arakawa et al. (1990, J. Neurosci. 10:3507-3515); increased sprouting of neurons may be detected by methods set forth in Pestronk et al. (1980, Exp. Neurol. 70:65-82) or Brown et al. (1981, Ann. Rev. Neurosci. 4:17-42); increased production of neuron-associated molecules may be measured by bioassay, enzymatic assay, antibody binding, Northern blot assay, etc., depending on the molecule to be measured; and motor neuron dysfunction may be measured by assessing the physical manifestation of motor neuron disorder, e.g., weakness, motor neuron conduction velocity, or functional disability.

In specific embodiments, motor neuron disorders that may be treated according to the invention include but are not limited to disorders such as infarction, infection, exposure to toxin, trauma, surgical damage, degenerative disease or malignancy that may affect motor neurons as well as other components of the nervous system, as well as disorders that selectively affect neurons such as amyotrophic lateral sclerosis, and including but not limited to progressive spinal muscular atrophy, progressive bulbar palsy, primary lateral sclerosis, infantile and juvenile muscular atrophy, progressive bulbar paralysis of childhood (Fazio-Londe syndrome), poliomyelitis and the post polio syndrome, and Hereditary Motorsensory Neuropathy (Charcot-Marie-Tooth Disease).

4.7.18 OTHER ACTIVITIES

A polypeptide of the invention may also exhibit one or more of the following additional activities or effects: inhibiting the growth, infection or function of, or killing, infectious agents, including, without limitation, bacteria, viruses, fungi and other parasites; effecting (suppressing or enhancing) bodily characteristics, including, without limitation, height, weight, hair color, eye color, skin, fat to lean ratio or other tissue pigmentation, or organ or body part size or shape (such as, for example, breast augmentation or diminution, change in bone form or shape); effecting biorhythms or circadian cycles or rhythms; effecting the fertility of male or female subjects; effecting the metabolism, catabolism, anabolism, processing, utilization, storage or elimination of dietary fat, lipid, protein, carbohydrate, vitamins, minerals, co-factors or other nutritional factors or component(s); effecting behavioral characteristics, including, without limitation, appetite, libido, stress, cognition (including cognitive disorders), depression (including depressive disorders) and violent behaviors; providing analgesic effects or other pain reducing effects; promoting differentiation and growth of embryonic stem cells in lineages other than hematopoietic lineages; hormonal or endocrine activity; in the case of enzymes, correcting deficiencies of the enzyme and treating deficiency-related diseases; treatment of hyperproliferative disorders (such as, for example, psoriasis); inimunoglobulin-like activity (such as, for example, the ability to bind antigens or complement); and the ability to act as an antigen in a vaccine composition to raise an immune response against such protein or another material or entity which is cross-reactive with such protein.

4.7.19 IDENTIFICATION OF POLYMORPHISMS

The demonstration of polymorphisms makes possible the identification of such polymorphisms in human subjects and the pharmacogenetic use of this information for diagnosis and treatment. Such polymorphisms may be associated with, e.g., differential predisposition or susceptibility to various disease states (such as disorders involving inflammation or immune response) or a differential response to drug administration, and this genetic information can be used to tailor preventive or therapeutic treatment appropriately. For example, the existence of a polymorphism associated with a predisposition to inflammation or autoimmune disease makes possible the diagnosis of this condition in humans by identifying the presence of the polymorphism.

Polymorphisms can be identified in a variety of ways known in the art which all generally involve obtaining a sample from a patient, analyzing DNA from the sample, optionally involving isolation or amplification of the DNA, and identifying the presence of the polymorphism in the DNA. For example, PCR may be used to amplify an appropriate fragment of genomic DNA which may then be sequenced. Alternatively, the DNA may be subjected to allele-specific oligonucleotide hybridization (in which appropriate oligonucleotides are hybridized to the DNA under conditions permitting detection of a single base mismatch) or to a single nucleotide extension assay (in which an oligonucleotide that hybridizes immediately adjacent to the position of the polymorphism is extended with one or more labeled nucleotides). In addition, traditional restriction fragment length polymorphism analysis (using restriction enzymes that provide differential digestion of the genomic DNA depending on the presence or absence of the polymorphism) may be performed. Arrays with nucleotide sequences of the present invention can be used to detect polymorphisms. The array can comprise modified nucleotide sequences of the present invention in order to detect the nucleotide sequences of the present invention. In the alternative, any one of the nucleotide sequences of the present invention can be placed on the array to detect changes from those sequences.

Alternatively a polymorphism resulting in a change in the amino acid sequence could also be detected by detecting a corresponding change in amino acid sequence of the protein, e.g., by an antibody specific to the variant sequence.

4.7.20 ARTHRITIS AND INFLAMMATION

The immunosuppressive effects of the compositions of the invention against rheumatoid arthritis is determined in an experimental animal model system. The experimental model system is adjuvant induced arthritis in rats, and the protocol is described by J. Holoshitz, et at., 1983, Science, 219:56, or by B. Waksman et al., 1963, Int. Arch. Allergy Appl. Immunol., 23:129. Induction of the disease can be caused by a single injection, generally intradermally, of a suspension of killed Mycobacterium tuberculosis in complete Freund's adjuvant (CFA). The route of injection can vary, but rats may be injected at the base of the tail with an adjuvant mixture. The polypeptide is administered in phosphate buffered solution (PBS) at a dose of about 1-5 mg/kg. The control consists of administering PBS only.

The procedure for testing the effects of the test compound would consist of intradermally injecting killed Mycobacterium tuberculosis in CFA followed by immediately administering the test compound and subsequent treatment every other day until day 24. At 14, 15, 18, 20, 22, and 24 days after injection of Mycobacterium CFA, an overall arthritis score may be obtained as described by J. Holoskitz above. An analysis of the data would reveal that the test compound would have a dramatic affect on the swelling of the joints as measured by a decrease of the arthritis score.

4.8 THERAPEUTIC METHODS

The compositions (including polypeptide fragments, analogs, variants and antibodies or other binding partners or modulators including antisense polynucleotides) of the invention have numerous applications in a variety of therapeutic methods. Examples of therapeutic applications include, but are not limited to, those exemplified herein.

4.8.1 EXAMPLE

One embodiment of the invention is the administration of an effective amount of the polypeptides or other composition of the invention to individuals affected by a disease or disorder that can be modulated by regulating the peptides of the invention. While the mode of administration is not particularly important, parenteral administration is preferred. An exemplary mode of administration is to deliver an intravenous bolus. The dosage of the polypeptides or other composition of the invention will normally be determined by the prescribing physician. It is to be expected that the dosage will vary according to the age, weight, condition and response of the individual patient. Typically, the amount of polypeptide administered per dose will be in the range of about 0.01 μg/kg to 100 mg/kg of body weight, with the preferred dose being about 0.1 μg/kg to 10 mg/kg of patient body weight. For parenteral administration, polypeptides of the invention will be formulated in an injectable form combined with a pharmaceutically acceptable parenteral vehicle. Such vehicles are well known in the art and examples include water, saline, Ringer's solution, dextrose solution, and solutions consisting of small amounts of the human serum albumin. The vehicle may contain minor amounts of additives that maintain the isotonicity and stability of the polypeptide or other active ingredient. The preparation of such solutions is within the skill of the art.

4.9 PHARMACEUTICAL FORMULATIONS AND ROUTES OF ADMINISTRATION

A protein or other composition of the present invention (from whatever source derived, including without limitation from recombinant and non-recombinant sources and including antibodies and other binding partners of the polypeptides of the invention) may be administered to a patient in need, by itself, or in pharmaceutical compositions where it is mixed with suitable carriers or excipient(s) at doses to treat or ameliorate a variety of disorders. Such a composition may optionally contain (in addition to protein or other active ingredient and a carrier) diluents, fillers, salts, buffers, stabilizers, solubilizers, and other materials well known in the art. The term “pharmaceutically acceptable” means a non-toxic material that does not interfere with the effectiveness of the biological activity of the active ingredient(s). The characteristics of the carrier will depend on the route of administration. The pharmaceutical composition of the invention may also contain cytokines, lymphokines, or other hematopoietic factors such as M-CSF, GM-CSF, TNF, IL-1, IL- 2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12, IL-13, IL-14, IL-15, IFN, TNF0, TNF1, TNF2, G-CSF, Meg-CSF, thrombopoietin, stem cell factor, and erythropoietin. In further compositions, proteins of the invention may be combined with other agents beneficial to the treatment of the disease or disorder in question. These agents include various growth factors such as epidermal growth factor (EGF), platelet-derived growth factor (PDGF), transforming growth factors (TGF-α and TGF-β), insulin-like growth factor (IGF), as well as cytokines described herein.

The pharmaceutical composition may further contain other agents which either enhance the activity of the protein or other active ingredient or complement its activity or use in treatment. Such additional factors and/or agents may be included in the pharmaceutical composition to produce a synergistic effect with protein or other active ingredient of the invention, or to minimize side effects. Conversely, protein or other active ingredient of the present invention may be included in formulations of the particular clotting factor, cytokine, lymphokine, other hematopoietic factor, thrombolytic or anti-thrombotic factor, or anti- inflammatory agent to minimize side effects of the clotting factor, cytokine, lymphokine, other hematopoietic factor, thrombolytic or anti-thrombotic factor, or anti-inflammatory agent (such as IL-IRa, IL-1 Hy1, IL-1 Hy2, anti-TNF, corticosteroids, immunosuppressive agents). A protein of the present invention may be active in multimers (e.g., heterodimers or homodimers) or complexes with itself or other proteins. As a result, pharmaceutical compositions of the invention may comprise a protein of the invention in such multimeric or complexed form.

As an alternative to being included in a pharmaceutical composition of the invention including a first protein, a second protein or a therapeutic agent may be concurrently administered with the first protein (e.g., at the same time, or at differing times provided that therapeutic concentrations of the combination of agents is achieved at the treatment site). Techniques for formulation and administration of the compounds of the instant application may be found in “Remington's Pharmaceutical Sciences,” Mack Publishing Co., Easton, Pa., latest edition. A therapeutically effective dose further refers to that amount of the compound sufficient to result in amelioration of symptoms, e.g., treatment, healing, prevention or amelioration of the relevant medical condition, or an increase in rate of treatment, healing, prevention or amelioration of such conditions. When applied to an individual active ingredient, administered alone, a therapeutically effective dose refers to that ingredient alone. When applied to a combination, a therapeutically effective dose refers to combined amounts of the active ingredients that result in the therapeutic effect, whether administered in combination, serially or simultaneously.

In practicing the method of treatment or use of the present invention, a therapeutically effective amount of protein or other active ingredient of the present invention is administered to a mammal having a condition to be treated. Protein or other active ingredient of the present invention may be administered in accordance with the method of the invention either alone or in combination with other therapies such as treatments employing cytokines, lymphokines or other hematopoietic factors. When co-administered with one or more cytokines, lymphokines or other hematopoietic factors, protein or other active ingredient of the present invention may be administered either simultaneously with the cytokine(s), lymphokine(s), other hematopoietic factor(s), thrombolytic or anti-thrombotic factors, or sequentially. If administered sequentially, the attending physician will decide on the appropriate sequence of administering protein or other active ingredient of the present invention in combination with cytokine(s), lymphokine(s), other hematopoietic factor(s), thrombolytic or anti-thrombotic factors.

4.9.1 ROUTES OF ADMINISTRATION

Suitable routes of administration may, for example, include oral, rectal, transmucosal, or intestinal administration; parenteral delivery, including intramuscular, subcutaneous, intramedullary injections, as well as intrathecal, direct intraventricular, intravenous, intraperitoneal, intranasal, or intraocular injections. Administration of protein or other active ingredient of the present invention used in the pharmaceutical composition or to practice the method of the present invention can be carried out in a variety of conventional ways, such as oral ingestion, inhalation, topical application or cutaneous, subcutaneous, intraperitoneal, parenteral or intravenous injection. Intravenous administration to the patient is preferred.

Alternately, one may administer the compound in a local rather than systemic manner, for example, via injection of the compound directly into a arthritic joints or in fibrotic tissue, often in a depot or sustained release formulation. In order to prevent the scarring process frequently occurring as complication of glaucoma surgery, the compounds may be administered topically, for example, as eye drops. Furthermore, one may administer the drug in a targeted drug delivery system, for example, in a liposome coated with a specific antibody, targeting, for example, arthritic or fibrotic tissue. The liposomes will be targeted to and taken up selectively by the afflicted tissue.

The polypeptides of the invention are administered by any route that delivers an effective dosage to the desired site of action. The determination of a suitable route of administration and an effective dosage for a particular indication is within the level of skill in the art. Preferably for wound treatment, one administers the therapeutic compound directly to the site. Suitable dosage ranges for the polypeptides of the invention can be extrapolated from these dosages or from similar studies in appropriate animal models. Dosages can then be adjusted as necessary by the clinician to provide maximal therapeutic benefit.

4.9.2 COMPOSITIONS/FORMULATIONS

Pharmaceutical compositions for use in accordance with the present invention thus may be formulated in a conventional manner using one or more physiologically acceptable carriers comprising excipients and auxiliaries which facilitate processing of the active compounds into preparations which can be used pharmaceutically. These pharmaceutical compositions may be manufactured in a manner that is itself known, e.g., by means of conventional mixing, dissolving, granulating, dragee-making, levigating, emulsifying, encapsulating, entrapping or lyophilizing processes. Proper formulation is dependent upon the route of administration chosen. When a therapeutically effective amount of protein or other active ingredient of the present invention is administered orally, protein or other active ingredient of the present invention will be in the form of a tablet, capsule, powder, solution or elixir. When administered in tablet form, the pharmaceutical composition of the invention may additionally contain a solid carrier such as a gelatin or an adjuvant. The tablet, capsule, and powder contain from about 5 to 95% protein or other active ingredient of the present invention, and preferably from about 25 to 90% protein or other active ingredient of the present invention. When administered in liquid form, a liquid carrier such as water, petroleum, oils of animal or plant origin such as peanut oil, mineral oil, soybean oil, or sesame oil, or synthetic oils may be added. The liquid form of the pharmaceutical composition may further contain physiological saline solution, dextrose or other saccharide solution, or glycols such as ethylene glycol, propylene glycol or polyethylene glycol. When administered in liquid form, the pharmaceutical composition contains from about 0.5 to 90% by weight of protein or other active ingredient of the present invention, and preferably from about 1 to 50% protein or other active ingredient of the present invention.

When a therapeutically effective amount of protein or other active ingredient of the present invention is administered by intravenous, cutaneous or subcutaneous injection, protein or other active ingredient of the present invention will be in the form of a pyrogen-free, parenterally acceptable aqueous solution. The preparation of such parenterally acceptable protein or other active ingredient solutions, having due regard to pH, isotonicity, stability, and the like, is within the skill in the art. A preferred pharmaceutical composition for intravenous, cutaneous, or subcutaneous injection should contain, in addition to protein or other active ingredient of the present invention, an isotonic vehicle such as Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, Lactated Ringer's Injection, or other vehicle as known in the art. The pharmaceutical composition of the present invention may also contain stabilizers, preservatives, buffers, antioxidants, or other additives known to those of skill in the art. For injection, the agents of the invention may be formulated in aqueous solutions, preferably in physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiological saline buffer. For transmucosal administration, penetrants appropriate to the barrier to be permeated are used in the formulation. Such penetrants are generally known in the art.

For oral administration, the compounds can be formulated readily by combining the active compounds with pharmaceutically acceptable carriers well known in the art. Such carriers enable the compounds of the invention to be formulated as tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions and the like, for oral ingestion by a patient to be treated. Pharmaceutical preparations for oral use can be obtained from a solid excipient, optionally grinding a resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries, if desired, to obtain tablets or dragee cores. Suitable excipients are, in particular, fillers such as sugars, including lactose, sucrose, mannitol, or sorbitol; cellulose preparations such as, for example, maize starch, wheat starch, rice starch, potato starch, gelatin, gum tragacanth, methyl cellulose, hydroxypropylmethyl-cellulose, sodium carboxymethylcellulose, and/or polyvinylpyrrolidone (PVP). If desired, disintegrating agents may be added, such as the cross-linked polyvinyl pyrrolidone, agar, or alginic acid or a salt thereof such as sodium alginate. Dragee cores are provided with suitable coatings. For this purpose, concentrated sugar solutions may be used, which may optionally contain gum arabic, talc, polyvinyl pyrrolidone, carbopol gel, polyethylene glycol, and/or titanium dioxide, lacquer solutions, and suitable organic solvents or solvent mixtures. Dyestuffs or pigments may be added to the tablets or dragee coatings for identification or to characterize different combinations of active compound doses.

Pharmaceutical preparations which can be used orally include push-fit capsules made of gelatin, as well as soft, sealed capsules made of gelatin and a plasticizer, such as glycerol or sorbitol. The push-fit capsules can contain the active ingredients in admixture with filler such as lactose, binders such as starches, and/or lubricants such as talc or magnesium stearate and, optionally, stabilizers. In soft capsules, the active compounds may be dissolved or suspended in suitable liquids, such as fatty oils, liquid paraffin, or liquid polyethylene glycols. In addition, stabilizers may be added. All formulations for oral administration should be in dosages suitable for such administration. For buccal administration, the compositions may take the form of tablets or lozenges formulated in conventional manner.

For administration by inhalation, the compounds for use according to the present invention are conveniently delivered in the form of an aerosol spray presentation from pressurized packs or a nebuliser, with the use of a suitable propellant, e.g., dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas. In the case of a pressurized aerosol the dosage unit may be determined by providing a valve to deliver a metered amount. Capsules and cartridges of, e.g., gelatin for use in an inhaler or insufflator may be formulated containing a powder mix of the compound and a suitable powder base such as lactose or starch. The compounds may be formulated for parenteral administration by injection, e.g., by bolus injection or continuous infusion. Formulations for injection may be presented in unit dosage form, e.g., in ampules or in multi-dose containers, with an added preservative. The compositions may take such forms as suspensions, solutions or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilizing and/or dispersing agents.

Pharmaceutical formulations for parenteral administration include aqueous solutions of the active compounds in water-soluble form. Additionally, suspensions of the active compounds may be prepared as appropriate oily injection suspensions. Suitable lipophilic solvents or vehicles include fatty oils such as sesame oil, or synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes. Aqueous injection suspensions may contain substances which increase the viscosity of the suspension, such as sodium carboxymethyl cellulose, sorbitol, or dextran. Optionally, the suspension may also contain suitable stabilizers or agents which increase the solubility of the compounds to allow for the preparation of highly concentrated solutions. Alternatively, the active ingredient may be in powder form for constitution with a suitable vehicle, e.g., sterile pyrogen-free water, before use.

The compounds may also be formulated in rectal compositions such as suppositories or retention enemas, e.g., containing conventional suppository bases such as cocoa butter or other glycerides. In addition to the formulations described previously, the compounds may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus, for example, the compounds may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.

A pharmaceutical carrier for the hydrophobic compounds of the invention is a co-solvent system comprising benzyl alcohol, a nonpolar surfactant, a water-miscible organic polymer, and an aqueous phase. The co-solvent system may be the VPD co-solvent system. VPD is a solution of 3% w/v benzyl alcohol, 8% w/v of the nonpolar surfactant polysorbate 80, and 65% w/v polyethylene glycol 300, made up to volume in absolute ethanol. The VPD co-solvent system (VPD:5W) consists of VPD diluted 1:1 with a 5% dextrose in water solution. This co-solvent system dissolves hydrophobic compounds well, and itself produces low toxicity upon systemic administration. Naturally, the proportions of a co-solvent system may be varied considerably without destroying its solubility and toxicity characteristics. Furthermore, the identity of the co-solvent components may be varied: for example, other low-toxicity nonpolar surfactants may be used instead of polysorbate 80; the fraction size of polyethylene glycol may be varied; other biocompatible polymers may replace polyethylene glycol, e.g. polyvinyl pyrrolidone; and other sugars or polysaccharides may substitute for dextrose. Alternatively, other delivery systems for hydrophobic pharmaceutical compounds may be employed. Liposomes and emulsions are well known examples of delivery vehicles or carriers for hydrophobic drugs. Certain organic solvents such as dimethylsulfoxide also may be employed, although usually at the cost of greater toxicity. Additionally, the compounds may be delivered using a sustained-release system, such as semipermeable matrices of solid hydrophobic polymers containing the therapeutic agent. Various types of sustained-release materials have been established and are well known by those skilled in the art. Sustained-release capsules may, depending on their chemical nature, release the compounds for a few weeks up to over 100 days. Depending on the chemical nature and the biological stability of the therapeutic reagent, additional strategies for protein or other active ingredient stabilization may be employed.

The pharmaceutical compositions also may comprise suitable solid or gel phase carriers or excipients. Examples of such carriers or excipients include but are not limited to calcium carbonate, calcium phosphate, various sugars, starches, cellulose derivatives, gelatin, and polymers such as polyethylene glycols. Many of the active ingredients of the invention may be provided as salts with pharmaceutically compatible counter ions. Such pharmaceutically acceptable base addition salts are those salts which retain the biological effectiveness and properties of the free acids and which are obtained by reaction with inorganic or organic bases such as sodium hydroxide, magnesium hydroxide, ammonia, trialkylamine, dialkylamine, monoalkylamine, dibasic amino acids, sodium acetate, potassium benzoate, triethanol amine and the like.

The pharmaceutical composition of the invention may be in the form of a complex of the protein(s) or other active ingredient(s) of present invention along with protein or peptide antigens. The protein and/or peptide antigen will deliver a stimulatory signal to both B and T lymphocytes. B lymphocytes will respond to antigen through their surface immunoglobulin receptor. T lymphocytes will respond to antigen through the T cell receptor (TCR) following presentation of the antigen by MHC proteins. MHC and structurally related proteins including those encoded by class I and class II MHC genes on host cells will serve to present the peptide antigen(s) to T lymphocytes. The antigen components could also be supplied as purified MHC-peptide complexes alone or with co-stimulatory molecules that can directly signal T cells. Alternatively antibodies able to bind surface immunoglobulin and other molecules on B cells as well as antibodies able to bind the TCR and other molecules on T cells can be combined with the pharmaceutical composition of the invention.

The pharmaceutical composition of the invention may be in the form of a liposome in which protein of the present invention is combined, in addition to other pharmaceutically acceptable carriers, with amphipathic agents such as lipids which exist in aggregated form as micelles, insoluble monolayers, liquid crystals, or lamellar layers in aqueous solution. Suitable lipids for liposomal formulation include, without limitation, monoglycerides, diglycer ides, sulfatides, lysolecithins, phospholipids, saponin, bile acids, and the like. Preparation of such liposomal formulations is within the level of skill in the art, as disclosed, for example, in U.S. Pat. Nos. 4,235,871; 4,501,728; 4,837,028; and 4,737,323, all of which are incorporated herein by reference.

The amount of protein or other active ingredient of the present invention in the pharmaceutical composition of the present invention will depend upon the nature and severity of the condition being treated, and on the nature of prior treatments which the patient has undergone. Ultimately, the attending physician will decide the amount of protein or other active ingredient of the present invention wi th which to treat each individual patient. Initially, the attending physician will administer low doses of protein or other active ingredient of the present invention and observe the patient's response. Larger doses of protein or other active ingredient of the present invention may be administered until the optimal therapeutic effect is obtained for the patient, and at that point the dosage is not increased further. It is contemplated that the various pharmaceutical compositions used to practice the method of the present invention should contain about 0.01 μg to about 100 μg (preferably about 0.1 μg to about 10 mg, more preferably about 0.1 μg to about 1 mg) of protein or other active ingredient of the present invention per kg body weight. For compositions of the present invention which are useful for bone, cartilage, tendon or ligament regeneration, the therapeutic method includes administering the composition topically, systematically, or locally as an implant or device. When administered, the therapeutic composition for use in this invention is, of course, in a pyrogen-free, physiologically acceptable form. Further, the composition may desirably be encapsulated or injected in a viscous form for delivery to the site of bone, cartilage or tissue damage. Topical administration may be suitable for wound healing and tissue repair. Therapeutically useful agents other than a protein or other active ingredient of the invention which may also optionally be included in the composition as described above, may alternatively or additionally, be administered simultaneously or sequentially with the composition in the methods of the invention. Preferably for bone and/or cartilage formation, the composition would include a matrix capable of delivering the protein-containing or other active ingredient-containing composition to the site of bone and/or cartilage damage, providing a structure for the developing bone and cartilage and optimally capable of being resorbed into the body. Such matrices may be formed of materials presently in use for other implanted medical applications.

The choice of matrix material is based on biocompatibility, biodegradability, mechanical properties, cosmetic appearance and interface properties. The particular application of the compositions will define the appropriate formulation. Potential matrices for the compositions may be biodegradable and chemically defined calcium sulfate, tricalcium phosphate, hydroxyapatite, polylactic acid, polyglycolic acid and polyanhydrides. Other potential materials are biodegradable and biologically well-defined, such as bone or dermal collagen. Further matrices are comprised of pure proteins or extracellular matrix components. Other potential matrices are nonbiodegradable and chemically defined, such as sintered hydroxyapatite, bioglass, aluminates, or other ceramics. Matrices may be comprised of combinations of any of the above mentioned types of material, such as polylactic acid and hydroxyapatite or collagen and tricalcium phosphate. The bioceramics may be altered in composition, such as in calcium-aluminate-phosphate and processing to alter pore size, particle size, particle shape, and biodegradability. Presently preferred is a 50:50 (mole weight) copolymer of lactic acid and glycolic acid in the form of porous particles having diameters ranging from 150 to 800 microns. In some applications, it will be useful to utilize a sequestering agent, such as carboxymethyl cellulose or autologous blood clot, to prevent the protein compositions from disassociating from the matrix.

A preferred family of sequestering agents is cellulosic materials such as alkylcelluloses (including hydroxyalkylcelluloses), including methylcellulose, ethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, hydroxypropyl-methylcellulose, and carboxymethylcellulose, the most preferred being cationic salts of carboxymethylcellulose (CMC). Other preferred sequestering agents include hyaluronic acid, sodium alginate, poly(ethylene glycol), polyoxyethylene oxide, carboxyvinyl polymer and poly(vinyl alcohol). The amount of sequestering agent useful herein is 0.5-20 wt %, preferably 1-10 wt % based on total formulation weight, which represents the amount necessary to prevent desorption of the protein from the polymer matrix and to provide appropriate handling of the composition, yet not so much that the progenitor cells are prevented from infiltrating the matrix, thereby providing the protein the opportunity to assist the osteogenic activity of the progenitor cells. In further compositions, proteins or other active ingredients of the invention may be combined with other agents beneficial to the treatment of the bone and/or cartilage defect, wound, or tissue in question. These agents include various growth factors such as epidermal growth factor (EGF), platelet derived growth factor (PDGF), transforming growth factors (TGF-α and TGF-β), and insulin-like growth factor (IGF).

The therapeutic compositions are also presently valuable for veterinary applications. Particularly domestic animals and thoroughbred horses, in addition to humans, are desired patients for such treatment with proteins or other active ingredients of the present invention. The dosage regimen of a protein-containing pharmaceutical composition to be used in tissue regeneration will be determined by the attending physician considering various factors which modify the action of the proteins, e.g., amount of tissue weight desired to be formed, the site of damage, the condition of the damaged tissue, the size of a wound, type of damaged tissue (e.g., bone), the patient's age, sex, and diet, the severity of any infection, time of administration and other clinical factors. The dosage may vary with the type of matrix used in the reconstitution and with inclusion of other proteins in the pharmaceutical composition. For example, the addition of other known growth factors, such as IGF I (insulin like growth factor I), to the final composition, may also effect the dosage. Progress can be monitored by periodic assessment of tissue/bone growth and/or repair, for example, X-rays, histomorphometric determinations and tetracycline labeling.

Polynucleotides of the present invention can also be used for gene therapy. Such polynucleotides can be introduced either in vivo or ex vivo into cells for expression in a mammalian subject. Polynucleotides of the invention may also be administered by other known methods for introduction of nucleic acid into a cell or organism (including, without limitation, in the form of viral vectors or naked DNA). Cells may also be cultured ex vivo in the presence of proteins of the present invention in order to proliferate or to produce a desired effect on or activity in such cells. Treated cells can then be introduced in vivo for therapeutic purposes.

4.9.3 EFFECTIVE DOSAGE

Pharmaceutical compositions suitable for use in the present invention include compositions wherein the active ingredients are contained in an effective amount to achieve its intended purpose. More specifically, a therapeutically effective amount means an amount effective to prevent development of or to alleviate the existing symptoms of the subject being treated. Determination of the effective amount is well within the capability of those skilled in the art, especially in light of the detailed disclosure provided herein. For any compound used in the method of the invention, the therapeutically effective dose can be estimated initially from appropriate in vitro assays. For example, a dose can be formulated in animal models to achieve a circulating concentration range that can be used to more accurately determine useful doses in humans. For example, a dose can be formulated in animal models to achieve a circulating concentration range that includes the IC 50 as determined in cell culture (i.e., the concentration of the test compound which achieves a half-maximal inhibition of the protein's biological activity). Such information can be used to more accurately determine useful doses in humans.

A therapeutically effective dose refers to that amount of the compound that results in amelioration of symptoms or a prolongation of survival in a patient. Toxicity and therapeutic efficacy of such compounds can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD 50 (the dose lethal to 50% of the population) and the ED 50 (the dose therapeutically effective in 50% of the population). The dose ratio between toxic and therapeutic effects is the therapeutic index and it can be expressed as the ratio between LD 50 and ED 50 . Compounds which exhibit high therapeutic indices are preferred. The data obtained from these cell culture assays and animal studies can be used in formulating a range of dosage for use in human. The dosage of such compounds lies preferably within a range of circulating concentrations that include the ED 50 with little or no toxicity. The dosage may vary within this range depending upon the dosage form employed and the route of administration utilized. The exact formulation, route of administration and dosage can be chosen by the individual physician in view of the patient's condition. See, e.g., Fingl et al., 1975, in “The Pharmacological Basis of Therapeutics”, Ch. 1 p. 1. Dosage amount and interval may be adjusted individually to provide plasma levels of the active moiety which are sufficient to maintain the desired effects, or minimal effective concentration (MEC). The MEC will vary for each compound but can be estimated from in vitro data. Dosages necessary to achieve the MEC will depend on individual characteristics and route of administration. However, HPLC assays or bioassays can be used to determine plasma concentrations.

Dosage intervals can also be determined using MEC value. Compounds should be administered using a regimen which maintains plasma levels above the MEC for 10-90% of the time, preferably between 30-90% and most preferably between 50-90%. In cases of local administration or selective uptake, the effective local concentration of the drug may not be related to plasma concentration.

An exemplary dosage regimen for polypeptides or other compositions of the invention will be in the range of about 0.01 μg/kg to 100 mg/kg of body weight daily, with the preferred dose being about 0.1 μg/kg to 25 mg/kg of patient body weight daily, varying in adults and children. Dosing may be once daily, or equivalent doses may be delivered at longer or shorter intervals.

The amount of composition administered will, of course, be dependent on the subject being treated, on the subject's age and weight, the severity of the affliction, the manner of administration and the judgment of the prescribing physician.

4.9.4 PACKAGING

The compositions may, if desired, be presented in a pack or dispenser device which may contain one or more unit dosage forms containing the active ingredient. The pack may, for example, comprise metal or plastic foil, such as a blister pack. The pack or dispenser device may be accompanied by instructions for administration. Compositions comprising a compound of the invention formulated in a compatible pharmaceutical carrier may also be prepared, placed in an appropriate container, and labeled for treatment of an indicated condition.

4.10 ANTIBODIES

Another aspect of the invention is an antibody that specifically binds the polypeptide of the invention. Such antibodies include monoclonal and polyclonal antibodies, single chain antibodies, chimeric antibodies, bifunctional/bispecific antibodies, humanized antibodies, human antibodies, and complementary determining region (CDR)-grafted antibodies, including compounds which include CDR and/or antigen-binding sequences, which specifically recognize a polypeptide of the invention. Preferred antibodies of the invention are human antibodies which are produced and identified according to methods described in WO93/11236, published Jun. 20, 1993, which is incorporated herein by reference in its entirety. Antibody fragments, including Fab, Fab′, F(ab′) 2 , and F v , are also provided by the invention. The term “specific for” indicates that the variable regions of the antibodies of the invention recognize and bind polypeptides of the invention exclusively (i.e., able to distinguish the polypeptide of the invention from other similar polypeptides despite sequence identity, homology, or similarity found in the family of polypeptides), but may also interact with other proteins (for example, S. aureus protein A or other antibodies in ELISA techniques) through interactions with sequences outside the variable region of the antibodies, and in particular, in the constant region of the molecule. Screening assays to determine binding specificity of an antibody of the invention are well known and routinely practiced in the art. For a comprehensive discussion of such assays, see Harlow et al. (Eds), Antibodies A Laboratory Manual; Cold Spring Harbor Laboratory; Cold Spring Harbor , N.Y. (1988), Chapter 6. Antibodies that recognize and bind fragments of the polypeptides of the invention are also contemplated, provided that the antibodies are first and foremost specific for, as defined above, full length polypeptides of the invention. As with antibodies that are specific for full length polypeptides of the invention, antibodies of the invention that recognize fragments are those which can distinguish polypeptides from the same family of polypeptides despite inherent sequence identity, homology, or similarity found in the family of proteins. Antibodies of the invention can be produced using any method well known and routinely practiced in the art.

Non-human antibodies may be humanized by any methods known in the art. In one method, the non-human CDRs are inserted into a human antibody or consensus antibody framework sequence. Further changes can then be introduced into the antibody framework to modulate affinity or immunogenicity.

Antibodies of the invention are useful for, for example, therapeutic purposes (by modulating activity of a polypeptide of the invention), diagnostic purposes to detect or quantitate a polypeptide of the invention, as well as purification of a polypeptide of the invention. Kits comprising an antibody of the invention for any of the purposes described herein are also comprehended. In general, a kit of the invention also includes a control antigen for which the antibody is immunospecific. The invention further provides a hybridoma that produces an antibody according to the invention. Antibodies of the invention are useful for detection and/or purification of the polypeptides of the invention.

Polypeptides of the invention may also be used to immunize animals to obtain polyclonal and monoclonal antibodies which specifically react with the protein. Such antibodies may be obtained using either the entire protein or fragments thereof as an immunogen. The peptide immunogens additionally may contain a cysteine residue at the carboxyl terminus, and are conjugated to a hapten such as keyhole limpet hemocyanin (KLH). Methods for synthesizing such pep tides are known in the art, for example, as in R. P. Merrifield, J. Amer. Chem. Soc. 85, 2149-2154 (1963); J. L. Krstenansky, et al., FEBS Lett. 211, 10 (1987).

Monoclonal antibodies binding to the protein of the invention may be useful diagnostic agents for the immunodetection of the protein. Neutralizing monoclonal antibodies binding to the protein may also be useful therapeutics for both conditions associated with the protein and also in the treatment of some forms of cancer where abnormal expression of the protein is involved. In the case of cancerous cells or leukemic cells, neutralizing monoclonal antibodies against the protein may be useful in detecting and preventing the metastatic spread of the cancerous cells, which may be mediated by the protein. In general, techniques for preparing polyclonal and monoclonal antibodies as well as hybridomas capable of producing the desired antibody are well known in the art (Campbell, A. M., Monoclonal Antibodies Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984); St. Groth et al., J. Immunol. 35:1-21 (1990); Kohler and Milstein, Nature 256:495497 (1975)), the trioma technique, the human B-cell hybridoma technique (Kozbor et al., Immunology Today 4:72 (1983); Cole et al., in Monoclonal Antibodies and Cancer Therapy, Alan R. Liss, Inc. (1985), pp. 77-96).

Any animal (mouse, rabbit, etc.) which is known to produce antibodies can be immunized with a peptide or polypeptide of the invention. Methods for immunization are well known in the art. Such methods include subcutaneous or intraperitoneal injection of the polypeptide. One skilled in the art will recognize that the amount of the protein encoded by the ORF of the present invention used for immunization will vary based on the animal which is immunized, the antigenicity of the peptide and the site of injection. The protein that is used as an immunogen may be modified or administered in an adjuvant in order to increase the protein's antigenicity. Methods of increasing the antigenicity of a protein are well known in the art and include, but are not limited to, coupling the antigen with a heterologous protein (such as globulin or -galactosidase) or through the inclusion of an adjuvant during immunization.

For monoclonal antibodies, spleen cells from the immunized animals are removed, fused with myeloma cells, such as SP2/0-Ag14 myeloma cells, and allowed to become monoclonal antibody producing hybridoma cells. Any one of a number of methods well known in the art can be used to identify the hybridoma cell which produces an antibody with the desired characteristics. These include screening the hybridomas with an ELISA assay, Western blot analysis, or radioimmunoassay (Lutz et al., Exp. Cell Research. 175:109-124 (1988)). Hybridomas secreting the desired antibodies are cloned and the class and subclass is determined using procedures known in the art (Campbell, A. M., Monoclonal Antibody Technology: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1984)). Techniques described for the production of single chain antibodies (U.S. Pat. No. 4,946,778) can be adapted to produce single chain antibodies to proteins of the present invention.

For polyclonal antibodies, antibody-containing antiserum is isolated from the immunized animal and is screened for the presence of antibodies with the desired specificity using one of the above-described procedures. The present invention further provides the above- described antibodies in delectably labeled form. Antibodies can be delectably labeled through the use of radioisotopes, affinity labels (such as biotin, avidin, etc.), enzymatic labels (such as horseradish peroxidase, alkaline phosphatase, etc.) fluorescent labels (such as FITC or rhodamine, etc.), paramagnetic atoms, etc. Procedures for accomplishing such labeling are well-known in the art, for example, see (Sternberger, L. A. et al., J. Histochem. Cytochem. 18:315 (1970); Bayer, E. A. et al., Meth. Enzym. 62:308 (1979); Engval, E. et al., Immunol. 109:129 (1972); Goding, J. W. J. Immunol. Meth. 13:215 (1976)).

The labeled antibodies of the present invention can be used for in vitro, in vivo, and in situ assays to identify cells or tissues in which a fragment of the polypeptide of interest is expressed. The antibodies may also be used directly in therapies or other diagnostics. The present invention further provides the above-described antibodies immobilized on a solid support. Examples of such solid supports include plastics such as polycarbonate, complex carbohydrates such as agarose and Sepharose®, acrylic resins and such as polyacrylamide and latex beads. Techniques for coupling antibodies to such solid supports are well known in the art (Weir, D. M. et al., “Handbook of Experimental Immunology” 4th Ed., Blackwell Scientific Publications, Oxford, England, Chapter 10 (1986); Jacoby, W. D. et al., Meth. Enzym. 34 Academic Press, N.Y. (1974)). The immobilized antibodies of the present invention can be used for in vitro, in vivo, and in situ assays as well as for immuno-affinity purification of the proteins of the present invention.

4.11 COMPUTER READABLE SEQUENCES

In one application of this embodiment, a nucleotide sequence of the present invention can be recorded on computer readable media. As used herein, “computer readable media” refers to any medium which can be read and accessed directly by a computer. Such media include, but are not limited to: magnetic storage media, such as floppy discs, hard disc storage medium, and magnetic tape; optical storage media such as CD-ROM; electrical storage media such as RAM and ROM; and hybrids of these categories such as magnetic/optical storage media. A skilled artisan can readily appreciate how any of the presently known computer readable mediums can be used to create a manufacture comprising computer readable medium having recorded thereon a nucleotide sequence of the present invention. As used herein, “recorded” refers to a process for storing information on computer readable medium. A skilled artisan can readily adopt any of the presently known methods for recording information on computer readable medium to generate manufactures comprising the nucleotide sequence information of the present invention.

A variety of data storage structures are available to a skilled artisan for creating a computer readable medium having recorded thereon a nucleotide sequence of the present invention. The choice of the data storage structure will generally be based on the means chosen to access the stored information. In addition, a variety of data processor programs and formats can be used to store the nucleotide sequence information of the present invention on computer readable medium. The sequence information can be represented in a word processing text file, formatted in commercially-available software such as WordPerfect and Microsoft Word, or represented in the form of an ASCII file, stored in a database application, such as DB2, Sybase, Oracle, or the like. A skilled artisan can readily adapt any number of data processor structuring formats (e.g. text file or database) in order to obtain computer readable medium having recorded thereon the nucleotide sequence information of the present invention.

By providing any of the nucleotide sequences SEQ ID NOs: 1-1104 or a representative fragment thereof; or a nucleotide sequence at least 95% identical to any of the nucleotide sequences of the SEQ ID NOs: 1-1104 in computer readable form, a skilled artisan can routinely access the sequence information for a variety of purposes. Computer software is publicly available which allows a skilled artisan to access sequence information provided in a computer readable medium. The examples which follow demonstrate how software which implements the BLAST (Altschul et al., J. Mol. Biol. 215:403-410 (1990)) and BLAZE (Brutlag et al., Comp. Chem. 17:203-207 (1993)) search algorithms on a Sybase system is used to identify open reading frames (ORFs) within a nucleic acid sequence. Such ORFs may be protein encoding fragments and may be useful in producing commercially important proteins such as enzymes used in fermentation reactions and in the production of commercially useful metabolites.

As used herein, “a computer-based system” refers to the hardware means, software means, and data storage means used to analyze the nucleotide sequence information of the present invention. The minimum hardware means of the computer-based systems of the present invention comprises a central processing unit (CPU), input means, output means, and data storage means. A skilled artisan can readily appreciate that any one of the currently available computer-based systems are suitable for use in the present invention. As stated above, the computer-based systems of the present invention comprise a data storage means having stored therein a nucleotide sequence of the present invention and the necessary hardware means and software means for supporting and implementing a search means. As used herein, “data storage means” refers to memory which can store nucleotide sequence information of the present invention, or a memory access means which can access manufactures having recorded thereon the nucleotide sequence information of the present invention.

As used herein, “search means” refers to one or more programs which are implemented on the computer-based system to compare a target sequence or target structural motif with the sequence information stored within the data storage means. Search means are used to identify fragments or regions of a known sequence which match a particular target sequence or target motif. A variety of known algorithms are disclosed publicly and a variety of commercially available software for conducting search means are and can be used in the computer-based systems of the present invention. Examples of such software includes, but is not limited to, Smith-Waterman, MacPattern (EMBL), BLASTN and BLASTA (NPOLYPEPTIDEIA). A skilled artisan can readily recognize that any one of the available algorithms or implementing software packages for conducting homology searches can be adapted for use in the present computer-based systems. As used herein, a “target sequence” can be any nucleic acid or amino acid sequence of six or more nucleotides or two or more amino acids. A skilled artisan can readily recognize that the longer a target sequence is, the less likely a target sequence will be present as a random occurrence in the database. The most preferred sequence length of a target sequence is from about 10 to 300 amino acids, more preferably from about 30 to 100 nucleotide residues. However, it is well recognized that searches for commercially important fragments, such as sequence fragments involved in gene expression and protein processing, may be of shorter length.

As used herein, “a target structural motif,” or “target motif,” refers to any rationally selected sequence or combination of sequences in which the sequence(s) are chosen based on a three-dimensional configuration which is formed upon the folding of the target motif. There are a variety of target motifs known in the art. Protein target motifs include, but are not limited to, enzyme active sites and signal sequences. Nucleic acid target motifs include, but are not limited to, promoter sequences, hairpin structures and inducible expression elements (protein binding sequences).

4.12 TRIPLE HELIX FORMATION

In addition, the fragments of the present invention, as broadly described, can be used to control gene expression through triple helix formation or antisense DNA or RNA, both of which methods are based on the binding of a polynucleotide sequence to DNA or RNA. Polynucleotides suitable for use in these methods are preferably 20 to 40 bases in length and are designed to be complementary to a region of the gene involved in transcription (triple helix—see Lee et al., Nucl. Acids Res. 6:3073 (1979); Cooney et al., Science 15241:456 (1988); and Dervan et al., Science 251:1360 (1991)) or to the mRNA itself (antisense—Olmno, J. Neurochem. 56:560 (1991); Oligodeoxynucleotides as Antisense Inhibitors of Gene Expression, CRC Press, Boca Raton, Fla. (1988)). Triple helix-formation optimally results in a shut-off of RNA transcription from DNA, while antisense RNA hybridization blocks translation of an mRNA molecule into polypeptide. Both techniques have been demonstrated to be effective in model systems. Information contained in the sequences of the present invention is necessary for the design of an antisense or triple helix oligonucleotide.

4.13 DIAGNOSTIC ASSAYS AND KITS

The present invention further provides methods to identify the presence or expression of one of the ORFs of the present invention, or homolog thereof, in a test sample, using a nucleic acid probe or antibodies of the present invention, optionally conjugated or otherwise associated with a suitable label.

In general, methods for detecting a polynucleotide of the invention can comprise contacting a sample with a compound that binds to and forms a complex with the polynucleotide for a period sufficient to form the complex, and detecting the complex, so that if a complex is detected, a polynucleotide of the invention is detected in the sample. Such methods can also comprise contacting a sample under stringent hybridization conditions with nucleic acid primers that anneal to a polynucleotide of the invention under such conditions, and amplifying annealed polynucleotides, so that if a polynucleotide is amplified, a polynucleotide of the invention is detected in the sample.

In general, methods for detecting a polypeptide of the invention can comprise contacting a sample with a compound that binds to and forms a complex with the polypeptide for a period sufficient to form the complex, and detecting the complex, so that if a complex is detected, a polypeptide of the invention is detected in the sample.

In detail, such methods comprise incubating a test sample with one or more of the antibodies or one or more of the nucleic acid probes of the present invention and assaying for binding of the nucleic acid probes or antibodies to components within the test sample.

Conditions for incubating a nucleic acid probe or antibody with a test sample vary. Incubation conditions depend on the format employed in the assay, the detection methods employed, and the type and nature of the nucleic acid probe or antibody used in the assay. One skilled in the art will recognize that any one of the commonly available hybridization, amplification or immunological assay formats can readily be adapted to employ the nucleic acid probes or antibodies of the present invention. Examples of such assays can be found in Chard, T., An Introduction to Radioimmunoassay and Related Techniques, Elsevier Science Publishers, Amsterdam, The Netherlands (1986); Bullock, G. R. et al., Techniques in Immunocytochemistry, Academic Press, Orlando, Fla. Vol. 1 (1982), Vol. 2 (1983), Vol. 3 (1985); Tijssen, P., Practice and Theory of immunoassays: Laboratory Techniques in Biochemistry and Molecular Biology, Elsevier Science Publishers, Amsterdam, The Netherlands (1985). The test samples of the present invention include cells, protein or membrane extracts of cells, or biological fluids such as sputum, blood, serum, plasma, or urine. The test sample used in the above-described method will vary based on the assay format, nature of the detection method and the tissues, cells or extracts used as the sample to be assayed. Methods for preparing protein extracts or membrane extracts of cells are well known in the art and can be readily be adapted in order to obtain a sample which is compatible with the system utilized.

In another embodiment of the present invention, kits are provided which contain the necessary reagents to carry out the assays of the present invention. Specifically, the invention provides a compartment kit to receive, in close confinement, one or more containers which comprises: (a) a first container comprising one of the probes or antibodies of the present invention; and (b) one or more other containers comprising one or more of the following: wash reagents, reagents capable of detecting presence of a bound probe or antibody.

In detail, a compartment kit includes any kit in which reagents are contained in separate containers. Such containers include small glass containers, plastic containers or strips of plastic or paper. Such containers allows one to efficiently transfer reagents from one compartment to another compartment such that the samples and reagents are not cross-contaminated, and the agents or solutions of each container can be added in a quantitative fashion from one compartment to another. Such containers will include a container which will accept the test sample, a container which contains the antibodies used in the assay, containers which contain wash reagents (such as phosphate buffered saline, Tris-buffers, etc.), and containers which contain the reagents used to detect the bound antibody or probe. Types of detection reagents include labeled nucleic acid probes, labeled secondary antibodies, or in the alternative, if the primary antibody is labeled, the enzymatic, or antibody binding reagents which are capable of reacting with the labeled antibody. One skilled in the art will readily recognize that the disclosed probes and antibodies of the present invention can be readily incorporated into one of the established kit formats which are well known in the art.

4.14 MEDICAL IMAGING

The novel polypeptides and binding partners of the invention are useful in medical imaging of sites expressing the molecules of the invention (e.g., where the polypeptide of the invention is involved in the immune response, for imaging sites of inflammation or infection). See, e.g., Kunkel et al., U.S. Pat. No. 5,413,778. Such methods involve chemical attachment of a labeling or imaging agent, administration of the labeled polypeptide to a subject in a pharmaceutically acceptable carrier, and imaging the labeled polypeptide in vivo at the target site.

4.15 SCREENING ASSAYS

Using the isolated proteins and polynucleotides of the invention, the present invention further provides methods of obtaining and identifying agents which bind to a polypeptide encoded by an ORF corresponding to any of the nucleotide sequences set forth in the SEQ ID NOs: 1-1104, or bind to a specific domain of the polypeptide encoded by the nucleic acid. In detail, said method comprises the steps of:

(a) contacting an agent with an isolated protein encoded by an ORF of the present invention, or nucleic acid of the invention; and

(b) determining whether the agent binds to said protein or said nucleic acid.

In general, therefore, such methods for identifying compounds that bind to a polynucleotide of the invention can comprise contacting a compound with a polynucleotide of the invention for a time sufficient to form a polynucleotide/compound complex, and detecting the complex, so that if a polynucleotide/compound complex is detected, a compound that binds to a polynucleotide of the invention is identified.

Likewise, in general, therefore, such methods for identifying compounds that bind to a polypeptide of the invention can comprise contacting a compound with a polypeptide of the invention for a time sufficient to form a polypeptide/compound complex, and detecting the complex, so that if a polypeptide/compound complex is detected, a compound that binds to a polynucleotide of the invention is identified.

Methods for identifying compounds that bind to a polypeptide of the invention can also comprise contacting a compound with a polypeptide of the invention in a cell for a time sufficient to form a polypeptide/compound complex, wherein the complex drives expression of a receptor gene sequence in the cell, and detecting the complex by detecting reporter gene sequence expression, so that if a polypeptide/compound complex is detected, a compound that binds a polypeptide of the invention is identified.

Compounds identified via such methods can include compounds which modulate the activity of a polypeptide of the invention (that is, increase or decrease its activity, relative to activity observed in the absence of the compound). Alternatively, compounds identified via such methods can include compounds which modulate the expression of a polynucleotide of the invention (that is, increase or decrease expression relative to expression levels observed in the absence of the compound). Compounds, such as compounds identified via the methods of the invention, can be tested using standard assays well known to those of skill in the art for their ability to modulate activity/expression.

The agents screened in the above assay can be, but are not limited to, peptides, carbohydrates, vitamin derivatives, or other pharmaceutical agents. The agents can be selected and screened at random or rationally selected or designed using protein modeling techniques.

For random screening, agents such as peptides, carbohydrates, pharmaceutical agents and the like are selected at random and are assayed for their ability to bind to the protein encoded by the ORF of the present invention. Alternatively, agents may be rationally selected or designed. As used herein, an agent is said to be “rationally selected or designed” when the agent is chosen based on the configuration of the particular protein. For example, one skilled in the art can readily adapt currently available procedures to generate peptides, pharmaceutical agents and the like, capable of binding to a specific peptide sequence, in order to generate rationally designed antipeptide peptides, for example see Hurby et al., Application of Synthetic Peptides: Antisense Peptides,” In Synthetic Peptides, A User's Guide, W. H. Freeman, NY (1992), pp. 289-307, and Kaspczak et al., Biochemistry 28:9230-8 (1989), or pharmaceutical agents, or the like.

In addition to the foregoing, one class of agents of the present invention, as broadly described, can be used to control gene expression through binding to one of the ORFs or EMFs of the present invention. As described above, such agents can be randomly screened or rationally designed/selected. Targeting the ORF or EMF allows a skilled artisan to design sequence specific or element specific agents, modulating the expression of either a single ORF or multiple ORFs which rely on the same EMF for expression control. One class of DNA binding agents are agents which contain base residues which hybridize or form a triple helix formation by binding to DNA or RNA. Such agents can be based on the classic phosphodiester, ribonucleic acid backbone, or can be a variety of sulfhydryl or polymeric derivatives which have base attachment capacity.

Agents suitable for use in these methods preferably contain 20 to 40 bases and are designed to be complementary to a region of the gene involved in transcription (triple helix—see Lee et al., Nucl. Acids Res. 6:3073 (1979); Cooney et al., Science 241:456 (1988); and Dervan et al., Science 251:1360 (1991)) or to the mRNA itself (antisense—Okano, J. Neurochem. 56:560 (1991); Oligodeoxynucleotides as Antisense Inhibitors of Gene Expression, CRC Press, Boca Raton, Fla. (1988)). Triple helix-formation optimally results in a shut-off of RNA transcription from DNA, while antisense RNA hybridization blocks translation of an mRNA molecule into polypeptide. Both techniques have been demonstrated to be effective in model systems. Information contained in the sequences of the present invention is necessary for the design of an antisense or triple helix oligonucleotide and other DNA binding agents.

Agents which bind to a protein encoded by one of the ORFs of the present invention can be used as a diagnostic agent. Agents which bind to a protein encoded by one of the ORFs of the present invention can be formulated using known techniques to generate a pharmaceutical composition.

4.16 USE OF NUCLEIC ACIDS AS PROBES

Another aspect of the subject invention is to provide for polypeptide-specific nucleic acid hybridization probes capable of hybridizing with naturally occurring nucleotide sequences. The hybridization probes of the subject invention may be derived from any of the nucleotide sequences SEQ ID NOs: 1-1104. Because the corresponding gene is only expressed in a limited number of tissues, a hybridization probe derived from of any of the nucleotide sequences SEQ ID NOs: 1-1104 can be used as an indicator of the presence of RNA of cell type of such a tissue in a sample.

Any suitable hybridization technique can be employed, such as, for example, in situ hybridization. PCR as described in U.S. Pat. Nos. 4,683,195 and 4,965,188 provides additional uses for oligonucleotides based upon the nucleotide sequences. Such probes used in PCR may be of recombinant origin, may be chemically synthesized, or a mixture of both. The probe will comprise a discrete nucleotide sequence for the detection of identical sequences or a degenerate pool of possible sequences for identification of closely related genomic sequences.

Other means for producing specific hybridization probes for nucleic acids include the cloning of nucleic acid sequences into vectors for the production of mRNA probes. Such vectors are known in the art and are commercially available and may be used to synthesize RNA probes in vitro by means of the addition of the appropriate RNA polymerase as T7 or SP6 RNA polymerase and the appropriate radioactively labeled nucleotides. The nucleotide sequences may be used to construct hybridization probes for mapping their respective genomic sequences. The nucleotide sequence provided herein may be mapped to a chromosome or specific regions of a chromosome using well known genetic and/or chromosomal mapping techniques. These techniques include in situ hybridization, linkage analysis against known chromosomal markers, hybridization screening with libraries or flow-sorted chromosomal preparations specific to known chromosomes, and the like. The technique of fluorescent in situ hybridization of chromosome spreads has been described, among other places, in Verma et al (1988) Human Chromosomes: A Manual of Basic Techniques, Pergamon Press, New York N.Y.

Fluorescent in situ hybridization of chromosomal preparations and other physical chromosome mapping techniques may be correlated with additional genetic map data. Examples of genetic map data can be found in the 1994 Genome Issue of Science (265:1981f). Correlation between the location of a nucleic acid on a physical chromosomal map and a specific disease (or predisposition to a specific disease) may help delimit the region of DNA associated with that genetic disease. The nucleotide sequences of the subject invention may be used to detect differences in gene sequences between normal, carrier or affected individuals.

4.17 PREPARATION OF SUPPORT BOUND OLIGONUCLEOTIDES

Oligonucleotides, i.e., small nucleic acid segments, may be readily prepared by, for example, directly synthesizing the oligonucleotide by chemical means, as is commonly practiced using an automated oligonucleotide synthesizer.

Support bound oligonucleotides may be prepared by any of the methods known to those of skill in the art using any suitable support such as glass, polystyrene or Teflon. One strategy is to precisely spot oligonucleotides synthesized by standard synthesizers. Immobilization can be achieved using passive adsorption (Inouye & Hondo, (1990) J. Clin. Microbiol. 28(6) 1469-72); using UV light (Nagata et al., 1985; Dahlen et al., 1987; Morrissey & Collins, (1989) Mol. Cell Probes 3(2) 189-207) or by covalent binding of base modified DNA (Keller et al., 1988; 1989); all references being specifically incorporated herein.

Another strategy that may be employed is the use of the strong biotin-streptavidin interaction as a linker. For example, Broude et al. (1994) Proc. Natl. Acad. Sci. USA 91(8) 3072-6, describe the use of biotinylated probes, although these are duplex probes, that are immobilized on streptavidin-coated magnetic beads. Streptavidin-coated beads may be purchased from Dynal, Oslo. Of course, this same linking chemistry is applicable to coating any surface with streptavidin. Biotinylated probes may be purchased from various sources, such as, e.g., Operon Technologies (Alameda, Calif.).

Nunc Laboratories (Naperville, Ill.) is also selling suitable material that could be used. Nunc Laboratories have developed a method by which DNA can be covalently bound to the microwell surface termed Covalink NH. CovaLink NH is a polystyrene surface grafted with secondary amino groups (>NH) that serve as bridge-heads for further covalent coupling. CovaLink Modules may be purchased from Nunc Laboratories. DNA molecules may be bound to CovaLink exclusively at the 5′-end by a phosphoramidate bond, allowing immobilization of more than 1 pmol of DNA (Rasmussen et al., (1991) Anal. Biochem. 198(1) 13842).

The use of CovaLink NH strips for covalent binding of DNA molecules at the 5′-end has been described (Rasmussen et al., (1991). In this technology, a phosphoramidate bond is employed (Chu et al., (1983) Nucleic Acids Res. 11(8) 6513-29). This is beneficial as immobilization using only a single covalent bond is preferred. The phosphoramidate bond joins the DNA to the CovaLink NH secondary amino groups that are positioned at the end of spacer arms covalently grafted onto the polystyrene surface through a 2 nm long spacer arm. To link an oligonucleotide to CovaLink NH via an phosphoramidate bond, the oligonucleotide terminus must have a 5′-end phosphate group. It is, perhaps, even possible for biotin to be covalently bound to CovaLink and then streptavidin used to bind the probes.

More specifically, the linkage method includes dissolving DNA in water (7.5 ng/ul) and denaturing for 10 min. at 95° C. and cooling on ice for 10 min. Ice-cold 0.1 M 1-methylimidazole, pH 7.0 (1-MeIm 7 ), is then added to a final concentration of 10 mM 1-MeIm 7 . A ss DNA solution is then dispensed into CovaLink NH strips (75 ul/well) standing on ice.

Carbodiimide 0.2 M 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC), dissolved in 10 mM 1-MeIm 7 , is made fresh and 25 ul added per well. The strips are incubated for 5 hours at 50° C. After incubation the strips are washed using, e.g., Nunc-Immuno Wash; first the wells are washed 3 times, then they are soaked with washing solution for 5 min., and finally they are washed 3 times (where in the washing solution is 0.4 N NaOH, 0.25% SDS heated to 50° C.).

It is contemplated that a further suitable method for use with the present invention is that described in PCT Patent Application WO 90/03382 (Southern & Maskos), incorporated herein by reference. This method of preparing an oligonucleotide bound to a support involves attaching a nucleoside 3′-reagent through the phosphate group by a covalent phosphodiester link to aliphatic hydroxyl groups carried by the support. The oligonucleotide is then synthesized on the supported nucleoside and protecting groups removed from the synthetic oligonucleotide chain under standard conditions that do not cleave the oligonucleotide from the support. Suitable reagents include nucleoside phosphoramidite and nucleoside hydrogen phosphorate.

An on-chip strategy for the preparation of DNA probe for the preparation of DNA probe arrays may be employed. For example, addressable laser-activated photodeprotection may be employed in the chemical synthesis of oligonucleotides directly on a glass surface, as described by Fodor et al. (1991) Science 251(4995) 767-73, incorporated herein by reference. Probes may also be immobilized on nylon supports as described by Van Ness et al. (1991) Nucleic Acids Res. 19(12) 3345-50; or linked to Teflon using the method of Duncan & Cavalier (1988) Anal. Biochem. 169(1) 104-8; all references being specifically incorporated herein.

To link an oligonucleotide to a nylon support, as described by Van Ness et al. (1991), requires activation of the nylon surface via alkylation and selective activation of the 5′-amine of oligonucleotides with cyanuric chloride.

One particular way to prepare support bound oligonucleotides is to utilize the light-generated synthesis described by Pease et al., (1994) PNAS USA 91(11) 5022-6, incorporated herein by reference). These authors used current photolithographic techniques to generate arrays of immobilized oligonucleotide probes (DNA chips). These methods, in which light is used to direct the synthesis of oligonucleotide probes in high-density, miniaturized arrays, utilize photolabile 5′-protected N-acyl-deoxynucleoside phosphoramidites, surface linker chemistry and versatile combinatorial synthesis strategies. A matrix of 256 spatially defined oligonucleotide probes may be generated in this manner.

4.18 PREPARATION OF NUCLEIC ACID FRAGMENTS

The nucleic acids may be obtained from any appropriate source, such as cDNAs, genomic DNA, chromosomal DNA, microdissected chromosome bands, cosmid or YAC inserts, and RNA, including mRNA without any amplification steps. For example, Sambrook et al. (1989) describes three protocols for the isolation of high molecular weight DNA from mammalian cells (p. 9.14-9.23).

DNA fragments may be prepared as clones in M13, plasmid or lambda vectors and/or prepared directly from genomic DNA or cDNA by PCR or other amplification methods. Samples may be prepared or dispensed in multiwell plates. About 100-1000 ng of DNA samples may be prepared in 2-500 ml of final volume.

The nucleic acids would then be fragmented by any of the methods known to those of skill in the art including, for example, using restriction enzymes as described at 9.24-9.28 of Sambrook et al. (1989), shearing by ultrasound and NaOH treatment.

Low pressure shearing is also appropriate, as described by Schriefer et al. (1990) Nucleic Acids Res. 18(24) 7455-6, incorporated herein by reference). In this method, DNA samples are passed through a small French pressure cell at a variety of low to intermediate pressures. A lever device allows controlled application of low to intermediate pressures to the cell. The results of these studies indicate that low-pressure shearing is a useful alternative to sonic and enzymatic DNA fragmentation methods.

One particularly suitable way for fragmenting DNA is contemplated to be that using the two base recognition endonuclease, CviJI, described by Fitzgerald et al. (1992) Nucleic Acids Res. 20(14) 3753-62. These authors described an approach for the rapid fragmentation and fractionation of DNA into particular sizes that they contemplated to be suitable for shotgun cloning and sequencing.

The restriction endonuclease CviJI normally cleaves the recognition sequence PuGCPy between the G and C to leave blunt ends. Atypical reaction conditions, which alter the specificity of this enzyme (CviJI**), yield a quasi-random distribution of DNA fragments form the small molecule pUC19 (2688 base pairs). Fitzgerald et al. (1992) quantitatively evaluated the randomness of this fragmentation strategy, using a CviJI** digest of pUC19 that was size fractionated by a rapid gel filtration method and directly ligated, without end repair, to a lac Z minus M13 cloning vector. Sequence analysis of 76 clones showed that CviJI** restricts pyGCPy and PuGCPu, in addition to PuGCPy sites, and that new sequence data is accumulated at a rate consistent with random fragmentation.

As reported in the literature, advantages of this approach compared to sonication and agarose gel fractionation include: smaller amounts of DNA are required (0.2-0.5 ug instead of 2-5 ug); and fewer steps are involved (no preligation, end repair, chemical extraction, or agarose gel electrophoresis and elution are needed

Irrespective of the manner in which the nucleic acid fragments are obtained or prepared, it is important to denature the DNA to give single stranded pieces available for hybridization. This is achieved by incubating the DNA solution for 2-5 minutes at 80-90° C. The solution is then cooled quickly to 2° C. to prevent renaturation of the DNA fragments before they are contacted with the chip. Phosphate groups must also be removed from genomic DNA by methods known in the art.

4.19 PREPARATION OF DNA ARRAYS

Arrays may be prepared by spotting DNA samples on a support such as a nylon membrane. Spotting may be performed by using arrays of metal pins (the positions of which correspond to an array of wells in a microtiter plate) to repeated by transfer of about 20 nl of a DNA solution to a nylon membrane. By offset printing, a density of dots higher than the density of the wells is achieved. One to 25 dots may be accommodated in 1 mm 2 , depending on the type of label used. By avoiding spotting in some preselected number of rows and columns, separate subsets (subarrays) may be formed. Samples in one subarray may be the same genomic segment of DNA (or the same gene) from different individuals, or may be different, overlapped genomic clones. Each of the subarrays may represent replica spotting of the same samples. In one example, a selected gene segment may be amplified from 64 patients. For each patient, the amplified gene segment may be in one 96-well plate (all 96 wells containing the same sample). A plate for each of the 64 patients is prepared. By using a 96-pin device, all samples may be spotted on one 8×12 cm membrane. Subarrays may contain 64 samples, one from each patient. Where the 96 subarrays are identical, the dot span may be 1 mm 2 and there may be a 1 mm space between subarrays.

Another approach is to use membranes or plates (available from NUNC, Naperville, Ill.) which may be partitioned by physical spacers e.g. a plastic grid molded over the membrane, the grid being similar to the sort of membrane applied to the bottom of multiwell plates, or hydrophobic strips. A fixed physical spacer is not preferred for imaging by exposure to flat phosphor-storage screens or x-ray films.

The present invention is illustrated in the following examples. Upon consideration of the present disclosure, one of skill in the art will appreciate that many other embodiments and variations may be made in the scope of the present invention. Accordingly, it is intended that the broader aspects of the present invention not be limited to the disclosure of the following examples. The present invention is not to be limited in scope by the exemplified embodiments which are intended as illustrations of single aspects of the invention, and compositions and methods which are functionally equivalent are within the scope of the invention. Indeed, numerous modifications and variations in the practice of the invention are expected to occur to those skilled in the art upon consideration of the present preferred embodiments. Consequently, the only limitations which should be placed upon the scope of the invention are those which appear in the appended claims.

All references cited within the body of the instant specification are hereby incorporated by reference in their entirety.

5.0 EXAMPLES

5.1 Example 1

Novel Nucleic Acid Sequences Obtained From Various Libraries

A plurality of novel nucleic acids were obtained from cDNA libraries prepared from various human tissues and in some cases isolated from a genomic library derived from human chromosome using standard PCR, SBH sequence signature analysis and Sanger sequencing techniques. The inserts of the library were amplified with PCR using primers specific for the vector sequences which flank the inserts. Clones from cDNA libraries were spotted on nylon membrane filters and screened with oligonucleotide probes (e.g., 7-mers) to obtain signature sequences. The clones were clustered into groups of similar or identical sequences. Representative clones were selected for sequencing.

In some cases, the 5′ sequence of the amplified inserts was then deduced using a typical Sanger sequencing protocol. PCR products were purified and subjected to fluorescent dye terminator cycle sequencing. Single pass gel sequencing was done using a 377 Applied Biosystems (ABI) sequencer to obtain the novel nucleic acid sequences. In some cases RACE (Random Amplification of cDNA Ends) was performed to further extend the sequence in the 5′ direction.

5.2 Example 2

Novel Nucleic Acids

The novel nucleic acids of the present invention of the invention were assembled from sequences that were obtained from a cDNA library by methods described in Example 1 above, and in some cases sequences obtained from one or more public databases. The nucleic acids were assembled using an EST sequence as a seed. Then a recursive algorithm was used to extend the seed EST into an extended assemblage, by pulling additional sequences from different databases (i.e., Hyseq's database containing EST sequences, dbEST version 114, gb pri 114, and UniGene version 101) that belong to this assemblage. The algorithm terminated when there was no additional sequences from the above databases that would extend the assemblage. Inclusion of component sequences into the assemblage was based on a BLASTN hit to the extending assemblage with BLAST score greater than 300 and percent identity greater than 95%.

Using PHRAP (Univ. of Washington) or CAP4 (Paracel), a full length gene cDNA sequence and its corresponding protein sequence were generated from the assemblage. Any frame shifts and incorrect stop codons were corrected by hand editing. During editing, the sequence was checked using FASTY and/or BLAST against Genbank (i.e., dbEST version 117, gb pri 117, UniGene version 117, Genepet release 117). Other computer programs which may have been used in the editing process were phredPhrap and Consed (University of Washington) and ed-ready, ed-ext and gc-zip-2 (H yseq, Inc.). The full-length nucleotide and amino acid sequences, including splice variants resulting from these procedures are shown in the Sequence Listing as SEQ ID NOS: 1-1104.

Table 1 shows the various tissue sources of SEQ ID NO: 1-1104.

The nearest neighbor results for SEQ ID NO: 1-1104 were obtained by a BLASTP version 2.0al 19MP-WashU search against Genpept release 118, using BLAST algorithm. The nearest neighbor result showed the closest homologue for SEQ ID NO: 1-1104 from Genpept (and contains the translated amino acid sequences for which the nucleic acid sequence encodes). The nearest neighbor results for SEQ ID NO: 1-1104 are shown in Table 2 below.

TABLE 1
		HYSEQ LIBRARY
TISSUE ORIGIN	RNA SOURCE	NAME	SEQ ID NOS:
adult brain	GIBCO	AB3001	4-5 7 29-30 35 42 52 55-56 90
			97 117 133-134 147 149 151 162
			170 174 177 193 201 222 250 258
			263 285-286 290 295 311-312
			323-324 330 336-337 339 348
			351-353 360 369 377 379 392 398
			408 415 459-461 480 489 496
			542-544 547 554 584-585 597 599
			606 609 611-616 620 623 649 666
			675-676 683 688 691-693 695-696
			706 727 735 748 753 756 759 767
			771 796 802 805-806 820 823-826
			829 838 840 846 869 895 919 924
			931 933 948 962-963 969 978-980
			984 997-998 1002 1010 1013 1020
			1046 1050-1051 1058 1063-1065
			1069 1081 1090
adult brain	GIBCO	ABD003	2-4 6-7 18-22 29-30 52-54 66 74
			82 88 93 98 100-102 104 107-110
			112-113 117 119 123 127-128
			133-135 142 145-147 150-152 157
			165 168 170 174 177 181-182 190
			193-195 200-202 209 211-215
			217-218 220 228 230 236 245-246
			250-252 262-264 269 272 274 278
			283-286 293-297 299-300 302
			305-311 313-314 321 323-327 331
			333-335 339-340 343-346 348
			350-352 358 363 369 383 392-393
			398 401 408-412 419 427 429-430
			434 437 443 449-450 457 459-462
			470 473 480 484-485 487-488
			495-496 500 502 505-506 517
			519-521 525 530-532 536 543-546
			549 554 559 563 568 582 586-587
			589-590 593 596 598-601 603
			608-609 611-614 616 619-621
			623-626 628-629 632 642-645 650
			653-656 664 666-667 672-673 677
			679-680 684-688 692-693 695 700
			705-708 711-712 717-719 722 724
			727 738 748 752-755 767 770-771
			774-775 778 786 792 796 798 801
			805-806 808 810 813 816 819 823
			833-834 838 840 846-847 856 859
			867 873 877 879 882-883 889 891
			904-906 909 915-916 919 921 931
			933 937 942 948 953 957 959 969
			971 974 976-979 983-984 996-997
			1002 1006-1010 1016 1023 1028
			1031 1034 1038 1041 1045-1047
			1058 1064 1067 1070 1076 1079-
			1080 1084 1090 1100
adult brain	Clontech	ABR001	3-4 17 21 25 35 52 57 66 78 82
			88 115-116 128 143 155 164 180
			191 262 274 309 319 338 373 398
			484 488 518 550 556 560 565 567
			593 607 624 687 692-694 715 724
			729 731 764 796 801 810 816
			825-826 833-836 921 928-929 970
			983 1010 1035 1045 1051 1073-
			1074 1090
adult brain	Clontech	ABR006	9-11 14 25 30 32-33 35 42 47 52
			57 66 69-70 88 93-94 100-102
			104 115-116 127 180 293-294 340
			371 469 483 530 598 706 742 798
			802 813 837 856 876 896 916 952
			955 975 1002 1007-1009 1014-
			1017 1034 1059 1071 1090
adult brain	Clontech	ABR008	1 3-4 6-11 14 17-20 22 25 27
			29-30 35 42-46 49 52-53 55 57
			60 63-64 66-67 70 72-76 79-85
			88-89 91-94 100-102 111-112
			114-118 127-129 136 138 141
			143-145 150 152 156-158 162-165
			171 177 180-187 190-191 194-195
			199-201 203-205 207-209 212-215
			217 219 222-226 228 237 241-243
			248-250 253 257 261 263-264
			266-267 271 274 276 278-279
			283-287 289 292-294 296-297
			299-300 305-307 309 311-312
			314-319 321 323-325 329-331
			334-340 343-345 348 351-353 355
			361 364 369 371 373-382 384 388
			392-393 398-401 404-405 409-418
			420-423 426-427 430 434-436 440
			442-443 446-448 450 452 457-462
			464-466 468-469 471-473 478 480
			484 487-488 490 496 499-500 503
			510-511 519-522 524-525 527-528
			541-544 546-547 550 552-557
			559-560 566 568-569 572 574-577
			579-580 582-583 586 589 593 595
			597-599 601 604 606 608-611
			613-614 617-619 622-628 630 632
			636 640-641 645 648-650 654 656
			658 662 664 668-670 673 675-677
			679 684 686-689 691-693 696 700
			706-711 715 717-719 723-725
			735-736 741 746 748 752 757 759
			761-764 766 770-772 774-782
			784-785 797-801 803-814 816
			820-822 824-827 831-834 837-847
			849-852 856 858 861 864 866 869
			872-877 880-884 888-889 893 895
			900 902-905 911-912 915-916
			919-921 924-925 931-932 934-953
			956 959-961 966-967 969-971
			974-976 982 984-985 989 995-999
			1002 1004 1006-1009 1012 1014-
			1017 1020 1022 1024-1025 1029
			1034-1036 1041 1044-1045 1047-
			1048 1055-1057 1059-1060 1063-
			1064 1066-1069 1071-1074 1076-
			1078 1082 1084 1086-1087 1090
			1094-1098 1101
adult brain	Clontech	ABR011	37-38 182 300 392 624 689-690
			748 893
adult brain	BioChain	ABR012	423 451 1061
adult brain	BioChain	ABR013	37-38 66 171 272 369 374-376
			515 530 757 1010 1104
adult brain	Invitrogen	ABR014	30 37-38 48 128 137 415 544 626
			670 762 952 960 1010 1094
adult brain	Invitrogen	ABR015	93 108-109 115-116 447 473 670
			1010
adult brain	Invitrogen	ABR016	37-38 52 1010 1024-1025
adult brain	Invitrogen	ABT004	9-11 19-20 22-23 29-30 35 44
			52-53 55-56 64 66 69 72 74 82
			102 112 133 135 150 156 164 176
			181-183 190 201 206 233 238 274
			279 284-286 301 330 334 349 351
			353-354 369-371 377-378 392 395
			398 405 416 423 427 434 437 450
			462 464-465 473 488 499 511 515
			522 526 542 554 559 579-580 612
			624 636 641-643 647-648 650
			655-656 675-676 687 692-693
			704-707 709 724-725 740 742 775
			779 798 802 804 809-810 8T2-813
			825-826 829 833-836 840 856 859
			863 877 882-883 894 914 919 921
			944 948 952 970 975 999 1002
			1024-1025 1031 1033-1034 1046-
			1047 1060 1068-1069 1073-1074
			1094-1095
adipocytes	Strategene	ADP001	4 9-11 52-53 64 73 102 104-105
			184-186 194 199 202 224 233 237
			279 295 297 299 309 315 325 352
			363-364 392 415 432 466 477-478
			502 519-521 528 530 543-544 564
			567 578-580 621 647 669 673
			682-683 687 689 692-693 695 713
			715-716 720 727 733 760 767 786
			788-791 825-826 829 908-909 918
			950 961 987-988 1004 1010 1012
			1019 1029 1035 1055-1056 1060-
			1061 1088 1099
adrenal gland	Clontech	ADR002	9-11 15 22 24-25 27 45-46 56-58
			64 73 84 89-90 98 100-101 105
			108-111 113 119 128 135 147 151
			157 165 167 171-172 177 190 193
			202 210 221 224 227 248-249 257
			264 272 277 279 285-287 297
			305-306 308 315 323-324 348 352
			361 385 393 396 398 403 416 418
			428-430 442 457-462 473 501 514
			522 530 533 554 560 568 583 589
			599 609-610 617-618 629 635 639
			652 654 656 663-664 668 677
			679-680 688 691 694 737 742 744
			748 760-761 765 801-802 804 810
			816 823-824 840 847 852 864 870
			877 898 907 913-914 916 921 933
			960-961 964 970 975 980 983 997
			1014-1015 1017 1020 1032-1033
			1035 1038 1055-1056 1059 1068-
			1069 1077 1088 1090 1096
adult heart	GIBCO	AHR001	3 6 9-11 18 22 24-25 27 31 34
			41 43 53 56-57 60-61 64 66 70
			74 80 82-83 85-86 88-90 104-105
			107-113 119-120 123 126 128-129
			131 133 136 143 146-147 150-154
			157-158 161-164 166-167 170-172
			177 180 182 184-187 190 193-194
			196-197 201 209 211-215 217 221
			224 228 231 236-238 250-253
			257-258 260 262-263 265 269 272
			274 281-286 288-289 292-297
			299-300 303 310 315 323-330 333
			335-339 341-342 346-347 352-354
			356-357 365-366 369 374-376 379
			383 391 393 395 398-400 403-404
			409-410 412 414-416 419 422-423
			427-428 430 435 437 443 445 449
			454-455 459-464 469 472-474 480
			487 489 495 497 502 506 511 513
			515 522 528 530-532 534-538
			542-546 548-549 554 556 560
			562-564 566 568 572 575-577
			579-580 582-583 586 588 591-592
			596-597 599-600 602 606 608-612
			614-615 620-622 632 645 648-650
			654 656-659 662 664-665 667-673
			677 679 686-689 692-695 698 704
			707-708 710-711 713 716-725
			727-728 731 735 738 743-744 746
			748 752-753 756-757 759 761
			764-765 772 774-776 778 782-783
			793 795 797-798 802 805-806 808
			810 813 815 817 820-823 833-834
			837-838 840 847 856 858-859 864
			866 881 885 888-889 891 895-897
			900 905 907 911 916-919 922-925
			928-931 933-934 937-938 940
			943-944 946-948 951 958 960-961
			967 970-972 977 982 988-989 996
			998 1007-1011 1014-1015 1020
			1022 1024-1027 1029 1034-1035
			1039-1040 1044-1046 1049-1050
			1055-1056 1059 1061 1064-1065
			1068-1070 1073-1074 1079 1087
			1090 1094 1100
adult kidney	GIBCO	AKD001	2-4 6 14 17 21-23 25 29 31-32
			35 37-38 41 43-45 47 49-50 53-
			55 65 68 71 73-74 78 81-83 88-
			89 97-98 104-111 117-118 120
			123 125 127-129 133 135-136 138
			141 143 145-148 150-151 154-155
			157 161-162 164-165 167 171-179
			184-187 190-194 197 199 201-204
			209 212-215 219 222 224 229-230
			232 236 238 240 243 247-252 254
			258 262-265 268-269 275-276 278
			281-283 285-290 292-296 298-303
			310-312 315-321 323-330 333-334
			339-340 343-345 351-354 358
			360-361 370 374-377 380 384
			393-394 396 400-401 403-404 411
			415 419-420 422 427 429-430 435
			437 439 446-447 449 455-457
			459-462 464 470 472 477 479-481
			485 488 490 493 495-497 499-504
			506 511 514 517 519-523 527-530
			533-540 542-546 549-550 552 554
			556 559 561-568 571-572 574-576
			578-581 583-585 587 591-593
			595-597 599-601 605 607 609
			612-614 616-622 624 627-629 632
			635-636 638 640-645 647-648
			650-651 656-657 659 663-665
			669-673 675-677 679-680 682-684
			686-689 691-696 698 700-701
			704-705 707-708 710 713-719 723
			726-728 730 732 735 738 742-744
			746-753 757 759-762 767 774-775
			777-778 781 783 786 788-791 796
			798-801 805-808 810 812-814
			821-824 829 831-832 835-836
			838-839 842-843 846 846 852 856
			859 867-868 870 872 881 885-889
			891-893 895-900 905-908 910-911
			914 916-917 919-920 922-924
			928-931 933-934 936-937 943
			947-948 950-951 957-958 960
			962-964 971 973 977-979 983-985
			989 996 998 1001-1003 1007-1010
			1013 1019-1020 1024-1025 1029
			1031 1039-1040 1043-1047 1049
			1057 1059 1063 1065 1068-1069
			1084 1087 1090 1094 1096 1098-
			1100
adult kidney	Invitrogen	AKT002	12 14 17-18 22 31 43-45 49 55-
			56 60 65 74 88 90 95 104 117
			119 123 127-128 146 161-162 178
			180 187 190 193 197 203 211-215
			221 229 261 263 270 275 279 284
			293-294 303-304 307 314 321 333
			343-345 377 391 405 419 427 445
			452 459-461 472 480 489 499 512
			522-523 534-535 537-538 554-555
			566 575-577 583 588 590-592 601
			606 612 614 621 628 636 639 644
			647 649 655 657 687 696 717-718
			732 740 744 752 756-757 762 774
			788-791 796 799-800 802 804 808
			815 823 825-826 835-837 842 848
			881 885 888 922-923 931 946 954
			962-963 971 975 983-984 989
			1002 1010 1014-1015 1020 1034
			1039 1041 1045-1046 1059 1068-
			1069 1094
adult lung	GIBCO	ALG001	5 18 24 35 44 54-55 67 82-83
			105 110 119 128 133 135 141 143
			145 150-151 162 178 187 202
			212-215 219 222-223 229 240 259
			284 293-294 298 300-301 312
			323-324 333 343-345 352 377 393
			399 403 427-428 447 450 458 473
			488 496 499 502 516-517 522-523
			530 537-538 543-544 546 551 565
			572 586 590 628 641-644 653 658
			664 666 668 674 677 679 683 688
			692-693 702 705 733 735 743 748
			751 753 761 769 775-778 783 796
			815 823 825-826 831 839 846 862
			879 889 897 909 931 962-963 970
			972 985 996 1010 1021-1022 1043
			1064 1069 1073-1074 1095 1100
lymph node	Clontech	ALN001	2 16 55 77 95 104 107 119 125
			135-136 149 155-156 177 201 204
			212-215 221-222 298 303 327 333
			361 403 409-410 422 477 481 488
			516 519-521 523 529 542-543 551
			564 590 600 615 650 663 692-693
			700 726 738 805-806 840 888 893
			915 933 955 964 1014-1015 1029
			1043 1064 1067
young liver	GIBCO	ALV001	15 24 29 32 35 45 47 65 67 71
			74 81-82 84 88 105 118 129 133
			150 152 157 167 184-186 190 194
			197 201 211 224 247 250-253 261
			268 278-279 284 298-301 305-306
			308 310-311 315 326 339 347 349
			360 379-380 382 401 403 412 414
			418 422 428-429 450 454 457 463
			472 485 493 497 499 526 534-536
			556 559 564 572 578 583-585
			587-589 591-592 596-597 606 609
			611 621 640 644 650 652 657 671
			673 684 688 691 695 700 717-718
			721-722 740 750 755 759 765 775
			778 782 788-791 799-801 805-806
			808 810 814 825-826 833-834
			845-846 848 885 888-889 898 900
			908-909 931 934 952 960 965 969
			973 985 1007-1010 1022 1029
			1034 1049 1052-1053 1069 1073-
			1074 1094
adult liver	Invitrogen	ALV002	6 29 32 47 49 71 74 82 88 102
			104 107-109 128 150 168 177-179
			191-192 203 209 219 227 237-239
			243 254 257 285-286 308 310 315
			322 328 333 340 346 354 384 389
			392 405-406 434 437 462 466 478
			499 505 511 519-522 532 534-535
			543 546 559 564 578 583 589 596
			605 608-609 613 633 635 659 665
			673 683 694 708 715 719 722 741
			752 757 759 762 775 778 782 786
			802 808 845 848 884 887-888 897
			909 916 931 951 962-963 967 975
			998 1005 1010 1014-1015 1020
			1052-1053 1065 1094 1099-1100
adult liver	Clontech	ALV003	358 684 752 808
adult ovary	Invitrogen	AOV001	3 5-6 8-11 14 17 22 25-26 29 31
			33 35 43-45 49-50 52-57 60 63-
			65 67 70-71 73 76 82-84 86 88-
			90 97-98 100-101 104-109 113
			117-120 122-123 125 127-129
			136-137 142-145 147 150-153
			155-158 161 163-168 170-171
			173-175 177-179 183-188 190-191
			193-195 197 199 201 203-209
			216-219 221-222 224 227 229-231
			237-240 242-243 245-246 248-249
			251-252 255 257 259 261-264
			266-267 269 272 274 276 278-282
			284-290 292 295-299 301-304
			307-308 310-311 314-318 320
			322-325 327-330 332-334 336-346
			348-357 359-360 363 365-366
			374-377 379-393 397-405 407
			409-411 413 416 418-419 427-430
			434-435 437 442 447 449 451
			456-457 459-462 464 467-468 470
			472 474-475 478-480 485 488 490
			494-496 498-500 503-504 506-509
			511 513-514 516-517 522-523
			528-535 537-542 544-549 552 554
			556-557 559-560 563-568 572-573
			575-580 583-595 597-601 603
			606-609 611 613-614 616-626 628
			630-633 635-638 640 642-645
			648-651 653-654 657-666 668-669
			672-673 675-677 679 682-684
			686-688 691-697 699-701 704
			707-708 712-713 717-719 721-723
			725 727 729-735 738-739 742-749
			751-753 756-759 761-762 764-765
			767-768 774-775 778-779 783-785
			787-792 795-797 801-806 808-809
			812-816 820-824 831-832 835-838
			842 845 847 852 854-855 858-860
			862-864 866-867 869 872-874
			879-880 884 886-889 891 894-903
			906-908 910-915 922-925 928-931
			933-934 937 939 942-944 946-948
			952 955 957-958 960 965 968
			970-973 976-979 981 983-984
			988-989 996 998 1002 1007-1011
			1013 1017 1019-1020 1022-1023
			1028-1029 1031 1033-1041 1046
			1049 1051 1055-1056 1061-1065
			1067-1069 1073-1074 1076 1079
			1084 1090 1095
adult placenta	Clontech	APL001	24 88 150 177 220 250 301 314
			403 439 457 545 575-576 611 634
			642-643 649 677 679 689 733 742
			891 1010 1059
adult placenta	Invitrogen	APL002	2 7 12 32 52-53 91 97 104 108-
			109 122 128 157 164-165 191 197
			309 407 409-410 412 422 430 453
			503 528 539-540 542-543 603 635
			640-641 663 675-676 692-693 701
			721 724 753 757759 775 828
			833-834 849 967 975 1007-1010
			1033 1035
adult spleen	GIBCO	ASP001	6 14 22 31 45 55 59-60 67 77 80
			88 105 108-110 120 122 125 147
			155 164-167 174 177 179 191 193
			205 209 211-215 222 229 233
			250-252 258 268 272 281 288-289
			297-298 300 309-310 320 330 333
			338 340 349 353 355-357 366 382
			393 405 412 414 416 422 426-428
			430 446 449 458 464 472-473 478
			495-496 502 507 515 517 530 533
			537-538 543 547 551 559 564 567
			569 591-593 599 606 608-610
			613-614 621 629 636 644-645 650
			664-665 670 677 679-681 683-684
			686 692-693 695 698 700 719 724
			730-732 734 737 748 757 759 765
			778-779 783 792 798 802 804 813
			829 852 859 863 866 879 885
			908-909 915-916 919 926 931 936
			948 959 961 964 985 991 1010
			1012 1020 1024-1025 1029 1039
			1041 1043 1049-1050 1054 1059
			1064 1076 1087 1104
adult testis	GIBCO	ATS001	3 7 22 43-45 65 71 84 89 100-
			101 104-105 107 111 120 128 135
			143 147 151 155-156 167 173
			176-177 187 197 201 222 227-228
			239-240 248-249 251-252 257-258
			261 276-279 262 289 292-294 297
			307 309 315 325 327-328 333 335
			339 346 352 356-357 395 403-405
			407 412 415 422 428 430 457 464
			468 472-473 487 489 494 496 499
			511 516 522 528 530 533 544 551
			554 562 590 597 600 603 606 608
			613 616 624 637 647 650 658 662
			664 668 677 679-680 695 704
			717-718 722-723 726-727 733
			748-749 753 759 761-762 776 778
			786 788-791 793 797 802 830 838
			850-851 864 897-898 911 916 919
			921 925 928-929 933 943 975
			977-979 981-983 997 1001 1007-
			1010 1020 1022 1028 1035 1059
			1063-1064 1069 1079 1089-1090
			1100
Genomic DNA	Research	BAC001	368 1042 1103
from BAC 63I18	Genetics
	(CITB BAC
	Library)
Genomic DNA	Research	BAC002	1102-1103
from BAC 393I6	Genetics
	(CITB BAC
	Library)
Genomic DNA	Research	BAC003	1042
from BAC 393I6	Genetics
	(CITB BAC
	Library)
adult bladder	Invitrogen	BLD001	21 36 55 87 89 98 104 117 157
			176 217-218 238 240 284 293-294
			303 313 330 349 353 392 411
			439-440 446 457 470 472 522 525
			551 578 583 594 605 616 653 664
			670 704 713 744 762 771 829 856
			874 878 888 960 1059
bone marrow	Clontech	BMD001	3 6 12 16 25 30-31 43-44 55 57-
			58 60 62 67 77 82 84 86 90 95
			98-101 103-105 110-111 113 115-
			116 119 121 128-130 135-136
			145-146 149 151-153 155-156 159
			162 167 172 174 177-180 187 190
			193 198-199 201 205 210-215 217
			219-220 224 230-231 241 243
			245-253 257 259-262 264 269 271
			278 280-281 285-286 290-296
			298-299 307 310-311 314-315 320
			327-328 333 335-339 347 352 354
			356-357 361 365 383 390 393
			399-400 402-404 415 419-420
			424-426 428-429 436 445 449
			456-457 459-462 464 469-470
			473-475 480 -483 487 495-497
			508-509 511 515 517-518 522-523
			528-529 533-540 543-544 551-552
			554 563-565 566 575-577 581 588
			590 593 598-601 609-611 614
			617-618 624 626-628 639-640
			642-645 650 656 660 663 668 672
			674 677 679 682 687 689 692-693
			695 701 704 713 716-719 721 723
			725 727 730 732 735 738 741-743
			748 750 752 755 759-760 768 795
			799-802 804-806 813 815 823-824
			831 833-834 852 861 867 886
			888-889 891 905-906 913 916-918
			930 936 944 951 956 960 968 971
			975 977 980-981 983 989-991 998
			1004 1007-1010 1017 1021 1029
			1033 1036 1039 1043 1046 1051
			1057 1059 1062-1064 1069-1070
			1084 1090 1093-1094 1097 1099
			1104
bone marrow	Clontech	BMD002	3 7-8 21 25-26 30 33 43-46 57
			59-60 70 76-78 88-90 95 99-101
			103-104 107-110 114 118 125 128
			139-140 146 149 152 165 167 190
			194 198 200 206 220 223 227 242
			245-246 257 259 266-267 271
			274-275 278 293-294 298 319 328
			330 335 338 353 356-357 361 366
			392 402-403 412 418 421-422 426
			445 449 458-462 474 496 499 503
			515 519-521 539-540 544 551 554
			556 563-564 569 574 577 589
			609-610 619 677 679 685 688 691
			704 724 730 736 739 743-744 747
			777-778 795 801-803 844 846 881
			889 912 916-917 926-927 950-951
			972-973 975 977 985 1004 1007-
			1011 1014-1017 1020 1023 1031
			1033 1037 1040 1043 1045-1047
			1057 1059-1060 1063 1067 1069
			1071 1073-1074 1084 1087 1090
			1097 1104
bone marrow	Clontech	BMD004	177 609 724
bone marrow	Clontech	BMD007	551 1010
adult colon	Invitrogen	CLN001	6 32 59 61 67 82 94 129 155 159
			177 184-186 266-267 292 313
			325-326 346 354 366 379 392
			409-410 427 464 470 472 511-512
			533-535 539-540 543 551 557 583
			605 608-610 640 663 670 680
			692-693 705 708 713 715 743-744
			752 757-758 829 842 859 874 885
			888 909 970 1004-1005 1010 1035
			1041 1045 1059
Mixture of 16	Various	CTL016	74
tissues-	Vendors*
mRNAs*
Mixture of 16	Various	CTL021	466 821-822 1094
tissues-mRNAs*	Vendors*
adult cervix	BioChain	CVX001	2-3 5 8 15 25 31 35 44-45 49 52
			54-55 57 61 71 73 84 88 90 93-
			94 100-101 104-105 107-110 113
			120 122 135-136 138 145-151 153
			156-157 165 167 170 177 180
			184-187 190-191 201 205 210
			217-219 221-222 233 237 248-249
			251-253 257-258 261-262 264-265
			269-270 277 279 281-282 284-286
			289 292-296 300 322 328 332-333
			336-337 343-346 352 354 362 365
			373-376 380 388 390-391 393-394
			396 404-405 411 413 415-416 421
			429-431 437-438 444 449
			458-461 464 474 477 480-481 483
			485 488 490 503 511 513 516-517
			519-521 528 532-533 536-540
			542-545 549-551 554 556 561
			563-565 567 572 575-576 582
			584-587 590-593 598-600 603
			605-606 609 614 619 621-625 631
			635 642-643 645 650-651 654
			656-657 659 663 665-666 670 672
			677 679 687 692-695 704-705 712
			715 721 724 726 733 735 741
			743-745 755-756 760 762 764 768
			771 787 802 813 818 823 828
			835-838 852 856 859 862 864 866
			870 886 889 891 900 903 905
			910-911 914 916 922-924 930-931
			933 948 954-955 958 960 969
			977-980 998 1000-1001 1006 1010
			1013 1017 1035 1039 1043-1044
			1050 1062-1064 1073-1074 1076
			1084 1087 1096-1097
diaphragm	BioChain	DIA002	414 464 673 1100
endothelial	Strategene	EDT001	2 4-6 8 13 15 24-28 33 44 48
cells			53-55 57 60 63 65 70 73 81-82
			84 86 88 90 99-101 105 108-110
			113 115-119 123 128 137-138
			146-149 151-153 160 162-164
			171-172 174-178 184-187 190-193
			197 199 201-203 207-209 211-215
			217-221 223 237-239 242-243
			248-252 255 257 261-262 265 269
			272 274 276 280 284-289 292-294
			297 299 303 308 310-312 316-319
			322 325 333 335-339 341-345 352
			355 360 362-364 374-379 389
			391-392 395-396 403 414 416 418
			420 422 427-430 435 443 452 455
			457 459-462 467 470-473 477 485
			488 490-492 495-496 499 501-503
			506-507 509 511 513-514 517
			519-523 527-530 533-535 539-545
			547 549 554 556 559 561-564 568
			570 572 575-576 579-580 583-585
			588 593 595-596 599-600 603
			606-609 611 613-615 617-622 626
			630 635-636 638-641 644 646-648
			651 656-657 660 662-665 670-671
			675-677 679-680 683-664 687 689
			691-701 704-708 710-713 716 719
			721-722 726-734 738 744-745
			748-749 751-753 756-759 761-763
			765 767 771 775-776 778-779
			782-786 788-792 796 798-802
			805-806 813-815 817-820 823-824
			827 829 833-834 837-838 842 846
			849 852 860 872-873 887-888 891
			894-896 900 905-906 908 910 914
			918-919 922-925 928-931 933-934
			936 940 943 947-948 951 955-956
			958 960-961 964-967 970-973
			975-976 978-980 983 985 988 998
			1002 1007-1010 1014-1015 1017
			1019-1020 1024-1025 1028 1032-
			1033 1035-1037 1040 1045-1046
			1049 1057 1059 1064 1066 1069
			1088 1090 1097 1099
Genomic clones	DNA from	EPM001	368 987 993-994 1042 1102-1103
from the short	Genetic
arm of	Research
chromosome 8
esophagus	BioChain	ESO002	53 177 545 577 687 695 1087
fetal brain	Clontech	FBR001	9-11 52 64 85 155 221 239 284
			361 392 552 700 719 744 918 941
			952 1010 1098
fetal brain	Clontech	FBR004	4 35 47 76 110 288 323-324 338
			350 352 373 469 490 530 852 898
			905 922-923 928-929 1077 1101
fetal brain	Clontech	FBR006	3 6-7 9-11 19-20 25 30 43 46 50
			52-53 55 57 64-65 70 72 75 80-
			82 84-85 91 95 98 100-101 104
			110-111 114-117 128 134 138 141
			147 150 157 162-163 169 171 182
			184-187 190 193-194 199 205
			212-215 219 222 225 237 243
			248-250 258 266-267 272 274 281
			284-286 292 300 305-306 309 312
			316-318 334 336-337 339 346 351
			356-357 361 371 373-376 378-379
			381 383 388 392 399 404 412 416
			418 420 426-428 441-444 447
			459-462 464 484 491-492 495
			502-503 511 524 528 543 546 549
			556-557 569 575-576 579-580 583
			589 597 602 608-610 622-623 625
			632 637 639 642-643 645-646 648
			650 654 656-658 677 679 686 688
			692-694 696 701 704 710 712
			717-718 720 723 730 735-736 740
			745 754 756 759 771 778 798
			803-804 808 820 832-838 840
			842-845 849 852 856 861-862 867
			873 875 877 879-889 900 905
			911-912 915-916 919 921 926 935
			943-945 948 950 952 956 960-963
			971 977 998-999 1004 1007-1010
			1016 1024-1025 1029 1031 1034
			1040 1046 1059-1061 1063 1066
			1069 1071-1072 1076 1082 1086-
			1088
fetal brain	Clontech	FBRS03	194 549 757 877
fetal brain	Invitrogen	FBT002	2 7 12 19-20 23 30-32 54-56 63
			81 92-93 104 108-109 112 117-
			118 135 138-140 157 164 168 183
			190 193 197 202 233 237-238
			248-249 266-267 272 274 300 310
			325-326 328 334 351-352 354 364
			372-373 382-383 392 401 420 430
			466 468 472-473 499-500 510 514
			525-526 532 539-540 542-543
			582-585 589 606 612 622-624 633
			635-636 641 647-649 653 656-657
			673 683 687-688 692-693 695 700
			702 710-716 733 740 744 757 759
			761 767 771 774 779 798 804 807
			809 817 825-826 833-834 838
			845-846 882-884 887 893-894 909
			911 924 947 952 961 964-965 970
			975-976 984 1002 1004 1007-1010
			1020 1032 1034-1035 1039-1040
			1045-1046 1048 1054 1059 1069
			1073 -1074
fetal heart	Invitrogen	FHR001	549 724 837 919
fetal kidney	Clontech	FKD001	3 44 60 65 68 76 96-97 105 110
			117 129 143 174 193 197 217 239
			248-249 261 277 296 300 328
			332-333 336-337 339 361 403 415
			464 483 506 518 529 539-540 542
			549 553 563 596 616 645 647 656
			677 679 695 734 776 792 818 828
			975 1002 1021 1045 1059 1061
			1066 1081 1093
fetal kidney	Clontech	FKD002	25 57 100-101 114 259 279 749
			820 1014-1015
fetal kidney	Invitrogen	FKD007	8 361 675-676 687 916 920 1010
fetal lung	Clontech	FLG001	44 71 100-101 119 147 224 236
			281 293-294 303 309 327 329 393
			400 403 430 470-471 517 527
			534-535 549 579-580 764 867 871
			889 895 918 999 1001 1035
fetal lung	Invitrogen	FLG003	6 17-18 25 46 49 52 57 82 100-
			101 104 106 141-142 149 157 162
			167 190 206 210 220 222 224 240
			258 279 300 322 339 343-345 352
			355 393 400 409-410 412 445
			450-451 458 490 515 537-538 549
			560 608 619 624 633 636 650
			675-676 702 704-705 712 715 779
			786 859 874 889 904-905 913 948
			980 999 1010 1032 1037 1045
			1059 1071 1104
fetal lung	Clontech	FLG004	7 139-140 421 528 820
fetal liver-	Soares	FLS001	2-5 15 17-18 23 25-26 29-31 33
spleen			35 43-47 49 51-57 59-60 63 65-
			66 73-74 76 80 82 84-86 89 91
			93 96-111 113-116 118-119 122
			126-128 130 133 135-144 146-153
			155-157 161-162 164-165 167
			174-175 177-180 183-187 189-194
			197-199 201-204 206 209 211-215
			217 222-224 228-229 237-238 240
			243-244 247-252 255 257 259
			261-274 276-282 284 287-290
			292-298 300-301 303 305-312
			314-318 322 325-328 333-339
			341-342 346 348 351-352 354
			356-357 360 363-364 366 372-377
			379 384 390-393 396-397 399-403
			409-412 414-415 418 420 422
			424-425 427-430 432 434-435
			443-450 452 456 458-461 463
			467-472 477 480-481 483 485-490
			493 495-497 499 503-504 506-509
			511-517 519-522 528-532 534-552
			554-561 563-568 572 574-580
			582-583 587 589-596 599-601 603
			606 608-615 617-624 626 632-637
			640-645 647-648 650-652 654 656
			658 661-665 667-670 673 675-677
			679-680 682-684 687-689 691-696
			700 702 704-707 713-714 716-719
			721 723-724 727-732 735 737-738
			741 743-745 747-748 750-753
			756-759 761-763 765 767 771
			774-776 778-779 786 788-792
			796-802 804-808 810-812 814
			816-817 820-823 825-826 833-839
			842 848-849 852 859 864 866-867
			877 879-881 884-889 891 895
			897-900 903 905 907 910-911 914
			916 918 924 927 931 934 936-937
			941 944 948 951 956 958 960-963
			967-972 974-975 977-980 984
			989-990 996-998 1003-1010 1014-
			1015 1017 1020 1022-1023 1028
			1031 1033 1035-1037 1039-1040
			1043-1050 1052-1053 1055-1057
			1059-1060 1062-1064 1066 1068-
			1071 1073-1074 1076 1079-1081
			1090 1092 1094 1096-1101 1104
normalized	Soares	FLS002	2 4 7 15 21-22 25-26 29 32 41
fetal liver-			44 47 49-52 54-57 59-60 65-66
spleen			70-71 73-74 76 84-87 95 97 100-
			101 103-104 107-110 112 114 117
			119 122 125-126 128-129 134-137
			139-141 143 145-147 149-150
			152-153 155-157 161 164-165
			167-168 171 174-175 177-179
			183-187 189-191 193-194 199
			201-206 209 212-217 219 222
			227-229 231-232 234 237-238
			240-243 247-253 259-263 266-267
			269 271-272 274 278 281-283
			285-290 293-294 296-301 303-310
			312 315-320 322 325 327-330 332
			334-338 341-346 348 351-354
			356-357 363-364 367 370 372 378
			380 382-385 389 391-393 396
			400-401 403-405 407-408 412
			415-416 418 421-422 426-429 432
			436 438 443 447-450 452 454
			457-461 464 466 468 470 475
			479-483 486-487 490 493 496-497
			503 508-509 511-512 517 519-522
			524 528 531 533-541 543-546 550
			552 554 556 558-559 561 563
			566-567 572 574 577 579-583
			586-587 589 591-593 597 600-602
			606 608-611 613-619 621-622 624
			626-628 630 633-634 637 640 645
			648 652-654 658 660-662 664-666
			668 671-672 677 679-680 682-684
			686 688 691-693 695 697-699 701
			704 708 711 713 715-719 721-723
			727-730 732-733 737 741 744-745
			747-748 751 753-754 756-759
			761-763 768 771 776 778 782 786
			788-792 794-796 798-803 812
			814-816 821-823 829 832-834
			837-839 845 847 849 852 861-862
			866-867 870-873 879-881 884-886
			888 893 895 897 900 903 905 908
			916 918 920 924-925 927 930-931
			933 935 937 946 948 950-951 954
			958 960-961 965 968-969 974-976
			978-979 989-991 996 998 1002
			1004 1007-1009 1013-1015 1017-
			1020 1024-1025 1029 1033-1037
			1039-1040 1044-1045 1047 1050
			1052-1053 1055-1057 1059 1062
			1066 1068-1070 1076 1079-1081
			1084 1090 1094 1097 1100
fetal liver-	Soares	FLS003	25 45 52 54 57 65-66 76 96 98
spleen			103 108-109 114 118 127 177 189
			219 268 665 753 1014-1015 1017
			1023 1047 1059 1068
fetal liver	Invitrogen	FLV001	2 17 29 44 47 49 52-55 68 82 86
			88 93 103 108-109 115-116 139-
			140 164 168 178-180 183 197 202
			209 227-228 231 238 243 245-246
			248-250 284 309 336-337 349 351
			354 367-368 384 401 409-410 416
			432 443 445 462 468 470 494 502
			514 522 542 546 557-558 560 566
			574 579-580 594 596 603 641 650
			654 661 664 673 675-676 680 682
			684 687 692-693 696 702-703 708
			730-731 759 775-776 778-779
			807-808 811 833-834 837 842 846
			848 872 884-885 916 940 947-948
			952 957 970 975 997 1002 1004
			1007-1010 1034-1035 1039-1040
			1045 1052-1053 1094 1096
fetal liver	Clontech	FLV002	358 685
fetal liver	Clontech	FLV004	28 47 52 230 257 278 361 522
			532 544 812 926 960 1003 1014-
			1015 1017 1045 1057
fetal muscle	Invitrogen	FMS001	32 34 49 52 56 71 86-87 93 104
			110 122 126 136-137 147 151 177
			193 204 237 245-246 266-267 274
			284 300 303 313 325 339 354 398
			409-410 414 417 445 459-461 472
			511 522 545 559 600 602 608 622
			657 667 673 675-677 679 687-688
			695 702 715 723-724 733 749 753
			759 772 786 788-791 805-806 810
			853 862 887 897 917 930 948 956
			960 967 988 1007-1010 1014-1015
			1019 1026-1027 1059 1073-1074
			1089-1090 1094 1100
fetal muscle	Invitrogen	FMS002	75 100-101 137 222 276 420 554
			687 721 772 956
fetal skin	Invitrogen	FSK001	1 3 6-8 12 14 18 24-25 28-29
			31-32 35 41 43 45 49 52-55 57
			61 67 78-79 82-83 85-86 93-94
			98 100-101 110 115-116 119 122-
			123 128 136 139-140 146 150 164
			167 178 180 183 189-191 193 195
			201 206 212-215 222 224 231
			236-237 243 248-249 257 260 265
			271 288-289 292 300 308-309 313
			325 327 329 336-337 339 343-346
			349 351 354 358 360 363 370 383
			386-367 391-392 396 400-401
			404-405 409-410 412-413 416 432
			434 437 443 448 450 454 459-461
			464-465 470 472-473 483 494 496
			499 501-502 507 509-511 516 527
			542 544 546 548-549 566 579-580
			593 595-596 603 605 609-610 615
			619-620 622 624 633 635-636 640
			647-648 653-654 657 662 664 673
			677 679-680 686-687 691 695 701
			706 713 715 720 723 729-731 743
			746-747 749 751-753 758 761 766
			779-780 786 788-792 797 802 808
			812 817 820 823 829 837-838
			841-842 845 852 856 867 871 878
			881 884-885 887 890-894 897
			900-901 905 908-909 911 914 918
			930 933 948 956-957 964 967 970
			972-975 989 1000 1007-1012
			1014-1015 1019-1020 1032 1034
			1036 1039-1041 1044-1047 1055-
			1056 1059 1061 1063 1066 1068
			1070 1073-1074 1077 1089-1090
			1096 1101
fetal skin	Invitrogen	FSK002	3 8 12 14 27 43 45 58 73 75 82
			88 100-101 107 110 124 128 189
			219 261 275 451 495 530 626 765
			798 844 1007-1010 1016 1023-
			1025 1033 1037 1040 1059-1061
			1067-1068 1081 1087
fetal spleen	BioChain	FSP001	26 265 598 614 797
umbilical cord	BioChain	FUC001	6-8 18 24 29 41 44-46 52-53 59
			63 65 67 77 80 82-84 86 88 90
			94 98 101 104-110 112 118-120
			128 133 135 137 141 147 156-157
			160 162 164 169 178 183 199 201
			212-215 222 230-231 237 250-253
			264-267 269 283 292 297-298 310
			315 323-325 328 338-339 343-345
			349-350 356-357 366 369 378 380
			383 385-387 391 394 397 403-404
			409-411 416 433-434 446 449 455
			459-461 464 473-475 477 480
			487-488 499-502 507 518 528 530
			533 543 546 549 560 564 573
			579-580 582-585 588 591-592 596
			599 612 617-620 623 627 635 640
			647 653 664 671 677 679 685
			687-688 692-693 695 704 711 713
			725 734-735 748 750-752 762
			765-766 778 783-786 801-802 804
			823 833-837 840 843 845 852 860
			886 893-894 897 905 907 913
			924-925 933-934 936 969 973
			976-977 988 1006 1010 1034 1036
			1040 1047 1049 1061 1063 1065
			1071 1088 1090 1094 1099
fetal brain	GIBCO	HFB001	3-4 12 14 19-22 25 27 29-32 35
			37-38 44-45 50 52-53 55 57 60
			63 73 82 88-90 93 98 105 108-
			109 112 115-117 123 125 127-129
			131-137 144 146-147 149 151-152
			156-157 160-162 164-165 167 170
			173-174 176-178 180-181 183-186
			190 193-194 197 200-201 204-205
			212-215 218-219 221-222 231
			242-243 245-246 248-258 261 264
			266-267 269 272 276 278 281
			283-286 288-289 291-297 299-301
			308 310 314 321 323-325 328-330
			332-333 338 340 346 349-350
			352-354 358 360 363 369 371
			374-376 379 381 392-393 397 401
			405 411 413-416 418 420 422
			427-430 434-435 443 447 450 454
			456 459-461 468 473 475 480 484
			487-490 495-496 499 504-506 509
			511 515 519-522 524-535 539-541
			543-545 547 549 554 556 561-562
			564 568 571-572 575-576 578-580
			583-586 590 593-595 597 599-603
			606 609-610 612 614 617-620
			622-625 635 639-647 650-651 654
			656-659 662 665-666 668 670
			673-674 677 679 682 684 686-689
			692-697 699-701 704 706-707 711
			714 717-719 721-723 726 729 735
			738 741-742 746 748 753 757 759
			761-762 767-768 770-771 775 778
			783 786 792 796-798 802-804
			810-811 816 823-824 827 829 832
			837-840 842 845-846 848 856 859
			864 882-883 889 891 896-898 900
			905 908-909 915-916 919 922-924
			930-931 933 936 939-941 943-944
			946 957-958 961 966 969 972
			975-979 983-984 995 997-998
			1002 1006 1010 1017 1028-1029
			1031-1034 1036 1039 1045-1047
			1049 1055-1056 1059-1060 1063-
			1065 1069 1076 1079 1084 1088
			1100 1104
macrophage	Invitrogen	HMP001	177 312 360 544 563 620 675-676
			1069
infant brain	Soares	IB2002	3-4 6-7 12 19-20 22 25 29-30 35
			44-45 50 52-53 56-57 65 70 74
			80 82 85 87-88 90 92-93 96 102
			104-105 108-109 112 117 123
			127-128 135 138-140 143-144
			146-147 149-150 152 156-157 161
			164 166 168 171 177 181-182
			184-187 190 193-194 199-201
			205-209 211-215 222 225-226 237
			239-240 242-243 248-252 255
			257-258 260-263 266-267 269
			272-274 284-286 288-290 293-294
			297 299-301 304 309 315 319
			323-325 328-334 336-337 339-340
			343-346 348-357 371 373 377 379
			381 385 389 396-398 401 405
			412-415 417-420 423 427 429 435
			439 443 447 453-454 456 462
			464-465 469 472-473 477-478 484
			488 493 496 499 510-511 515
			519-521 525-528 530 533-535
			539-540 545 547 549-550 555 557
			560-561 563 571 573 575-576
			579-581 583 588-589 597 603-604
			606 609-612 614-615 617-619
			621-623 625 628 632-633 635-637
			640-641 646-649 653-656 664 666
			668 673-677 679 684 686-687
			691-694 702 704 706-707 712-713
			721 724 735 739-740 743-744
			746-748 752-753 757-759 762
			770-771 774 778 797-802 807-809
			812-813 819 821-827 829 838 840
			842-843 852-859 861 863-864
			872-873 877 879 882-883 885-886
			888 893 897-898 900 907-911
			915-916 919 921 924 931 937 939
			941 946 948 951-952 959 964 969
			971 973 975 977-979 984-985 995
			997 1003-1004 1006-1010 1019
			1022 1031-1032 1035 1039 1046
			1052-1053 1055-1056 1058-1060
			1064 1071 1073-1074 1078 1090
			1094 1097-1098 1104
infant brain	Soares	IB2003	4 7 12 19-20 22-23 25 30 44 49-
			50 55 57 76 82-83 88-89 127-129
			146 150 152-153 164 177 181 190
			194 205 209 211-215 226 239
			248-252 261 263 266-267 269
			272-273 278 283 289 293-294 297
			299-301 328 330-331 340 346 348
			352-353 356-357 360 371 398 401
			411-412 414 418 443 459-461
			464-465 469 480491-492 496 509
			530-531 549 554 560 567 574
			579-580 583 589 597 604 606
			608-610 619 622-623 633 637 641
			649 664 666 684 686-687 692-693
			697 699 705 712 721 725 731 744
			746 798 803-804 809 812 816
			833-834 838 840-842 849 856 859
			863 882-883 885 888 891 895 898
			909-910 915 918-919 930-931 941
			948 974 978-979 995 1004 1017
			1023 1034 1037 1040 1046-1047
			1055-1056 1068-1069 1078 1090
			1094
infant brain	Soares	IBM002	47 50 226 239 285-286 331 338
			348 499 641 692-693 717-718 819
			853 898 948 984 995 1010 1017
			1087
infant brain	Soares	IBS001	7 44 120 226 239 300 330 349
			351 353-354 504 636 653 802 819
			840 877 882-883 885 919 948 955
			961 977 1007-1009 1014-1015
			1032
lung	Strategene	LFB001	6 29 43 101 104 110 120 136 146
fibroblast			167 172 177 190 193 202 210
			212-215 217-219 238 241 243 262
			276-277 284 292 308 325 343-345
			374-376 395 403 429 432 459-461
			478-480 495 522 528-532 534
			539-541 554 561 588 621 640
			642-643 651 662-663 671 687
			692-693 695 712-713 721 727 741
			753 759 761 771 801-802 823
			825-827 829 884 912 918 922-923
			936 969 975 985 1001 1010 1024-
			1025 1033
lung tumor	Invitrogen	LGT002	2 5 14-15 17-18 21 25 31-33 36
			41 43-44 49 53-54 57 62-63 71
			73 77 83 88 97 100-101 104 106-
			110 117-118 124-125 128 136 142
			146-151 154-157 161-162 164-165
			167 170-172 174 177-180 183-186
			189-190 193 196-198 201 205 209
			216 219 222 229 231 233 240
			242-243 248-252 261-262 264-265
			272-273 278 284 288-289 292 296
			298 300 303 307-308 310-311 322
			325 327 329 334-335 339 343-346
			349 351 354 365-366 378 382 388
			392-393 396 399-400 402-403
			406-407 412 418-423 427 434 436
			447 449-450 457-462 468-469
			471-473 475 481 485 487-488
			494-497 499 509 511 513-514 517
			523-524 526-530 534-535 537-540
			542-546 549 551 559 563-565 567
			572 575-576 582-583 587 589
			591-592 595 597-598 603 605-607
			609 611-612 614 617-619 621-622
			624 634 637 640 642-643 648 650
			653-654 656 658 664-666 668 671
			673-674 677 679-680 682 684
			686-688 692-695 697 700-701 706
			708 710-712 714 717-718 721 726
			729 733 735-738 740 743-744 748
			752-753 756-760 762-763 767 774
			776 778 784-786 793 795-796 798
			801 805-806 808 815 823-824 828
			830-831 833-834 837 841-842
			845-847 856 859 861 867 884-885
			887-889 891 893 897 903 905 908
			911 916 924 931 937 942 947-948
			955 957-960 966 968 970-971 973
			975 978-979 985 990 995 998
			1002 1007-1011 1019 1028-1029
			1031 1034-1036 1039-1041 1043-
			1045 1047 1055-1056 1059 1068-
			1069 1073-1075 1084 1087 1090
			1092 1094 1099
lymphocytes	ATCC	LPC001	12 21 25 30 44 55 57 63 73 77-
			78 86 88 95 97 100-101 104-105
			119 121-122 125 128-129 149 177
			190 201-202 205 212-215 224
			232-236 239 250 253 265 279 290
			292-294 298 300 305-306 308
			314-315 325 327 333 336-338
			341-342 372 379 401 407 439 448
			459-461 464 470 473-474 477 485
			487 507 523 534 551 554 561
			566-568 577 583 591-592 596
			608-610 615 662 677 679 683 688
			691-695 721 738 743 767 796 824
			829-830 832 837 847 852 866 888
			894 905 915 921 926 931 934 940
			948 950 955 959-961 980-983 998
			1002 1007 1009-1010 1029 1035-
			1036 1038 1040-1041 1047 1057
			1059 1069 1071 1073-1074 1076-
			1077 1079 1084 1087 1096
leukocyte	GIBCO	LUC001	1-3 6-7 13-15 21-22 25 27-31
			43-44 53-58 60-61 73 77 79 82-
			84 86 88-90 95-99 104-105 108-
			109 111 113 115-116 119-125
			127-128 130 133 135-138 142-146
			148-153 155 157-159 161-165 170
			173-175 177-178 183-187 190-193
			197 201-202 204-206 209 211-215
			217 222-224 228-230 232-235
			242-243 247 250-253 255 258
			260-262 265 269 272-274 278-279
			281 283-284 288-290 292-298 300
			304-306 311 314-315 320 322-328
			333-334 338-339 341-346 349 351
			356-357 360 364-366 374-378 380
			382 388 390 392 399-404 409-410
			413-420 422-423 427-430 433 435
			437 440 443 449-450 452 454-462
			464 468-470 472-474 477 480 483
			485 487-488 495-496 499 502 504
			511 513-515 517 519-521 523 528
			530-545 548-549 551-556 562-564
			566-577 579-581 583 587 589-601
			603 606-612 614 617-619 622-623
			628-629 632 635-636 640 642-643
			645 647-649 651 654 657-658 660
			662 664 672-677 679-680 682-683
			686-689 691-697 699 702 705-707
			710-711 713 715 717-719 721-724
			726 729 731 735 738 741-743
			747-748 750-751 753 757 759 761
			767 774-775 778-780 782 786
			795-796 799-802 804-806 811-812
			814 818 821-822 824-826 829-834
			841 847 852 857 859-863 865-867
			869 871-872 875 884 887-889 891
			894-895 900 905 908-910 912-916
			920-926 928-932 937 942 944 946
			948 951-952 954-955 959-960
			962-965 967 970-974 977-983
			989-990 996 998 1002-1003 1007-
			1010 1012 1014-1020 1023-1025
			1029 1031 1033-1037 1039 1043-
			1047 1049-1051 1055-1057 1059-
			1060 1063-1064 1067 1069-1071
			1076-1077 1084 1090 1094 1100
leukocyte	Clontech	LUC003	45 78 104-105 129 135 150-151
			155 177 212-215 229 251-252 257
			265 285-286 298-299 301 308 310
			325 343-345 351 361 380 400 448
			457 459-461 487 533 536 541-542
			564 590-592 602 636 639 677 679
			689 802 808 859 872 905 926-927
			931 965 1036 1043 1047 1049
			1067
melanoma from	Clontech	MEL004	9-11 18 24 45 53-54 56 60 86
cell line			89-90 104 119 122 128 130 141
ATCC # CRL			155 164 173 177 201 203 221 223
1424			243 251-252 264 278 290 292 325
			339 341-342 346 418-419 421 443
			462 471 474 485 517 530 541 544
			554 563 568 572 590 601 619 621
			645 663 672 677 679 686-687 713
			719 726 738 744 757 763 777
			788-791 796 825-827 838 845 852
			861 879 884 889 903-904 906
			915-916 925 928-929 934 943 968
			970 983 1001 1010 1020 1024-
			1025 1035 1045 1057 1076-1077
			1084 1094 1097
mammary gland	Invitrogen	MMG001	1-2 4-5 7 12 14 17-16 21 23 29
			32-33 35-36 41 44 49 52-56 61
			63 66-68 70-71 79 82 86 88-89
			97 104 108-111 115-118 122 125-
			129 136-138 148 150 153 156
			159-160 162 164 168-169 172 177
			180 183-186 190-191 193-194 197
			202 204 209 212-216 222 224 229
			231 233 238 248-250 263 265-267
			274 279 284 295 300 305-306 310
			312-318 322 325-326 328-330 334
			336-337 339 341-342 346 349
			351-354 356-358 361 364 370
			377-378 388-389 392 396 400-401
			404 406-414 416-418 420 422 427
			431-432 434 439 443 445 450 454
			464 468 470-473 478 488 491-492
			494 499 502-507 512-514 522 527
			539-543 548-549 551 554-555 557
			563 566 574 578-585 589 591-592
			596 603 606 608-610 613 622-624
			633 635-636 640-641 644 647-648
			650 652-653 657 664-665 671-676
			680-684 687-688 692-694 696
			701-702 704-705 711 713 715
			720-721 724 727 731 733 744
			746-747 752 757-764 766-767 774
			776 778-780 784-791 796-797
			801-808 810-812 820-822 825-826
			828-831 833-834 837-838 842 848
			856 859 861-862 872-874 879-881
			885-888 896-897 901 905-907
			911-912 914 921 924 930-931 937
			943-944 948-949 951-952 960-970
			973 975-976 988 997 1004 1006-
			1010 1013-1015 1020 1024-1025
			1032-1035 1039-1042 1044-1045
			1049 1059-1060 1067 1069 1071
			1073-1074 1079 1096
induced neuron	Strategene	NTD001	3 13 31 44 48 50 53 55 96 98
cells			166 171 207-208 217 221 224 242
			262 272 289 323-325 332 418
			459-462 464 476 484 506 511 541
			543 560 623 640 672 677 679
			729-731 744 761 793 882-883 895
			912 936 943 964 978-979 981 984
			1001 1010 1039 1049 1103
retinoid acid	Strategene	NTR001	56 105 180 300 359 415 686-687
induced			888
neuronal cells
neuronal cells	Strategene	NTU001	5 7 9-11 48 64 66 80 88-90 128
			139-140 144 162 177 180 184-186
			193 212-215 248-250 274 279 284
			300 325 340 382 384 399 427 455
			476 543 589 635 641 664 687
			692-693 713 753 757 811 837 874
			908 915 924 936 961 973 985-986
			1001 1007-1010 1019-1020 1040
			1045 1055-1056 1068 1073-1074
			1090 1103
pituitary	Clontech	PIT004	52 82 93 104 128 744 784-785
gland			962-963 1002 1010 1068
placenta	Clontech	PLA003	43 528 591-592 970 1007-1009
			1059
prostate	Clontech	PRT001	3 31 46 55-56 73 104 108-110
			135-136 143 151 163 171 174
			179-180 187 196 201 206-208 222
			295 327 333 336-337 343-345 392
			413 451 455 473 505 546 556 559
			575-576 590 597 625 632 650-651
			675-676 689 713 721 733 742 750
			784-785 801 814 831 885 887 891
			922-923 931 948 962-963 977 985
			1003 1023 1031 1034 1039 1050
			1052-1053 1057
rectum	Invitrogen	REC001	14 17 41 46 61 68 82 88 94 104
			115-116 120 122 138 142 157 191
			212-215 222 243 261 265 279 300
			305-306 310 323-325 329 336-337
			351 401-403 405 409-410 422 432
			434 440 446-447 450 454 458 474
			504 510 528 534 536 566 588 594
			598 635-636 647 673 683 708 711
			721-722 753 756 759 764 775
			797-798 802 819 828 842 848 861
			867 874 876-878 894 909 914 930
			934 961 1007-1010 1013 1024-
			1025 1040-1041 1045 1059 1063
			1065
salivary gland	Clontech	SAL001	6 24 27 33 84 111 122 147 161
			168 170 175 210 230 245-246
			248-249 266-267 301 314 329 443
			446 448 452 455 472 485 494 511
			533 550 564 591-592 612 620 650
			704 708 713 728 734 744 755 792
			839 861 870 924 971-972 978-979
			988 1005 1010 1023 1026-1027
			1036 1049 1063 1069
skin	ATCC	SFB001	1010 1090
fibroblast
skin	ATCC	SFB002	420 713 943
fibroblast
skin	ATCC	SFB003	391 808 1040
fibroblast
small	Clontech	SIN001	7 13 44 71 82 86 88 90 97 100-
intestine			101 104 108-109 119 126 128 152
			177 190 193 196 198 218 222 224
			230 235 239 245-246 261 268 281
			288 303 305-306 310 328 338 363
			392-393 397 402 434 448 464 483
			514 527 542 544 551 572 577
			579-580 586 589 591-592 599 606
			622 684-685 687 695-696 714-716
			732 744 778 786 805-806 820 837
			858 888 908 924 937 954 969
			1006 1010 1016 1031 1033 1039
			1041 1064 1077 1088 1090 1094
			1098
skeletal	Clontech	SKM001	2 34 86 88 98 114 126 133 144
muscle			162 177-178 212-215 325 339
			355-357 398 457 464 470 481 515
			590 609 637 650 669 677 679 708
			735 752 804-806 810 823 917-918
			958 974 988 1011 1019 1026-1027
			1033 1100 1104
skeletal	Clonetech	SKMS03	1100
muscle
spinal cord	Clontech	SPC001	45 52 60 65 93 100-101 104 107
			122 141 147 161 163 180 183 187
			193 199 201 212-215 218 222 224
			226 231 235 243 245-246 269 284
			293-294 300 302 307 315 327-328
			330 336-337 352-353 355 361
			363-364 391 394 398 400 403
			412-413 418 433-434 437 459-461
			465 473 475 494 505 517 519-521
			532-534 539-540 543 546 549
			556-557 560 569 583 591-593 604
			606 611 621 629 631 657 672 677
			679-680 685 701 727 741 748 754
			761 764 766 771 774 786 792 798
			805-806 817 823 840 859 863
			881-883 904 914 931 933 973 984
			997 1002 1010 1013 1017 1020
			1040 1043-1044 1046 1049 1058
			1065 1090 1097-1098
adult spleen	Clontech	SPLc01	21 98 100-101 105 530 551 1010
			1020 1023 1034 1045-1047 1061
			1077 1087
stomach	Clontech	STO001	31 35 77 100-101 104 120 136
			149 153 210 274 297 308 329
			343-345 406 423 464 466 469 531
			606 647 651 653-654 663 696 700
			720 730 752 760 824 859 897
			924-925 949 967 970-971 1012
			1039 1049 1059
Mixture of 16	Various	SUP002	4 37-38 47-48 94 108-109 472
tissues-	Vendors*		544 859 1010 1016 1102
mRNAs*
Mixture of 16	Various	SUP005	88 99 108-109 115-116 195 547
tissues-	Vendors*		1010 1064 1078
mRNAs*
Mixture of 16	Various	SUP008	19-20 24 82 98 104 115-116 157
tissues-	Vendors*		326 705 852 952 1068 1073-1074
mRNAs*
Mixture of 16	Various	SUP009	12 43 104 472 850-851 1010 1040
tissues- mRNAs	Vendors*		1043 1090
thalamus	Clontech	THA002	6-7 14 24 29 37-38 52 61 66 69
			93-94 115-116 132 151 157 182
			200 206 224 243 283 285-286 325
			333 348 363 398 409-411 420 458
			466 468 478 512 568 575-576
			584-585 648 684 686-688 691 694
			722 744 786 818 833-834 839-840
			846 861-862 866 882-883 897 909
			939 970 995 1035 1045 1059
thymus	Clonetech	THM001	3 6 15 24 31 44 84 104 119 139-
			140 142 145 157 161 167 177 180
			183 191 201 210 212-215 221 223
			229 231 243 260 262 266-267 281
			289 298 316-318 323-325 327 335
			339 346 352 380 392 400 404 412
			419 423 430 443 446 450-451
			459-461 464 468-469 481 485 487
			494 509 511 513 530 536 543-544
			549 551 555-556 563 569 572 577
			584-585 598 614 617-618 654 670
			673 677 679 686 702 717-718 721
			748 755 762 792 796 802 805-806
			808 829-831 847 852 861 866 884
			895 898 902 905 907-908 910-911
			916 961 967 972 1010 1019 1039
			1057 1059 1073-1074
thymus	Clontech	THMc02	7 15 23 25-26 33 43-46 55 57 73
			76 84 88-90 98 104-105 110 119
			128-130 135 138-141 144 146
			148-150 157 162 171 174 178 180
			187 190 193-194 199 201 205-206
			209 212-215 224 229-230 241-242
			245-246 248-249 251-253 262-263
			272 280-281 283 289 308-310 312
			314 325 328 333 336-339 347 349
			351 355-357 360 380 382 385 390
			400 404 409-410 415 422-423
			427-428 434-437 441 443 447-449
			451 456-463 471 473-474 481 483
			485 495 508 514 519-521 526 533
			539-541 544 546 549 555-556 566
			575-576 583 593-594 598 600 615
			620 628 636 645 648 650 654 656
			662 668 673 675-677 679 686
			695-696 702 704 727 729 762-763
			768 778 786 798 803-806 811 820
			829-830 833-834 852 861 864-866
			875 885 887 895 900 905 909 912
			916 924-933 951-952 957 961
			970-971 974 980 998 1007-1010
			1012 1014-1015 1017 1020 1024-
			1025 1029 1036 1040-1041 1045
			1047 1050 1060 1064 1067-1069
			1076 1079-1080 1082 1087 1090
			1092-1093 1097-1098
thyroid gland	Clontech	THR001	5 8 14-15 17 21 23 27 31-32 36
			41 44-46 55-56 58 67 71 73-74
			85 88-89 95 98 104-105 107-110
			117-120 124-126 128-129 132
			146-148 150-152 156 158-159
			161-162 164-168 170-171 174-175
			177-178 180 183-187 190-191 193
			199 201-202 204 210-215 222 224
			238 240 245-246 251-252 264 270
			272 278-279 281 283 287 289
			292-294 296-300 305-306 308
			312-313 320 325 327-329 333 339
			343-346 348 350 352 363 372-376
			378 380 382 391 393 405 411-413
			416 418 422 424-425 427 429 434
			443 445 447 449 454 457 464 469
			479-480 483 487 489 508-509 511
			513 518 523 526-530 532 534
			542-545 552 559-560 563 565-566
			572-573 575-577 579-580 583 593
			595-596 600-601 609-610 613-614
			616 619-621 624 626 628-629 635
			640 644 648 650-651 653 665-666
			668-669 674 677 679 688 691-693
			698 702 708 712 721 726-727 732
			735 738 740-741 743 745 747 757
			759-760 766-768 777 783-786
			794-795 801-802 805-806 813 815
			817-818 820 823 825-826 831-832
			835-836 838-839 845 850-853 856
			859-862 866-870 879 881 886 889
			893 897-898 903 906 908 911 920
			922-923 925 931 935 937 944
			947-948 950 952 961-963 973
			976-979 989 996 1004 1010 1020-
			1022 1024-1025 1032 1035 1039
			1055-1057 1060-1062 1064 1071
			1084 1098
trachea	Clontech	TRC001	4 25 45 50 57 88 98 119-120 128
			146 148 165 170 236 255 264 269
			274 284 289 303 363 384 403 495
			544 551 563 579-580 599 603 609
			619 622 734 764 769 788-791 802
			897 904 918 922-923 927 971
			1002 1075 1077 1096
uterus	Clontech	UTR001	60 82 94 112 120 122 126 147
			165 167 173 177 180 187 193 197
			201 205 217-218 236 278 287 310
			338 346 404 435 457 464 518 530
			542 557 562 599 616 621 624 683
			697 699 706 738 764 796 813 859
			908 948 969 977 1000 1010 1013
			1033-1034 1065
*The 16 tissue-mRNAs and their vendor source, are as follows: 1) Normal adult brain mRNA (Invitrogen), 2) normal adult kidney mRNA (Invitrogen), 3) normal adult liver mRNA (Invitrogen), 4) normal fetal brain mRNA (Invitrogen), 5) normal fetal kidney mRNA (Invitrogen), 6) normal fetal liver mRNA (Invitrogen), 7) normal fetal skin mRNA (Invitrogen), 8) human adrenal gland mRNA (Clontech), 9) human bone marrow mRNA (Clontech),
#10) human leukemia lymphablastic mRNA (Clontech), 11) human thymus mRNA (Clontech), 12) human lymph node mRNA (Clontech), 13) human spinal cord mRNA (Clontech), 14) human thyroid mRNA (Clontech), 15) human esophagus mRNA (BioChain), 16) human conceptional umbilical cord mRNA (BioChain).

TABLE 2
	CORRESPONDING
	SEQ ID NO. IN			SMITH-
SEQ	U.S. Ser. No.	ACCESSION		WATERMAN	%
ID NO	09/552,317	NUMBER	DESCRIPTION	SCORE	IDENTITY
1	152	AL122081	Homo sapiens	1930	100
			hypothetical protein
2	167	AF212921	Mus musculus MMTV	484	94
			receptor variant 1
3	205	Z75330	Homo sapiens nuclear	6492	99
			protein SA-1
4	210	AL008583	Homo sapiens	2133	99
			dJ327J16.3 (supported
			by GENSCAN, FGENES and
			GENEWISE)
5	225	AK000381	Homo sapiens unnamed	1028	98
			protein product
6	226	AK000418	Homo sapiens unnamed	1747	100
			protein product
7	264	AF156598	Mus musculus p53-	997	65
			regulated DDA3
8	268	AK001463	Homo sapiens unnamed	1131	100
			protein product
9	293	AB033039	Homo sapiens KIAA1213	2438	100
			protein
10	293	AB033039	Homo sapiens KIAA1213	1510	74
			protein
11	293	AB033039	Homo sapiens KIAA1213	2415	98
			protein
12	302	AK001184	Homo sapiens unnamed	2830	99
			protein product
13	311	AB021643	Homo sapiens	2761	99
			gonadotropin inducible
			transcription
			repressor-3
14	352	AL122089	Homo sapiens	593	100
			hypothetical protein
15	358	AC007228	Homo sapiens BC37295_1	1178	44
16	368	L29154	Homo sapiens	439	84
			immunoglobulin heavy
			chain VDJ region
17	393	AB037780	Homo sapiens KIAA1359	1439	74
			protein
18	477	AK000404	Homo sapiens unnamed	1177	99
			protein product
19	508	L22557	Rattus norvegicus	1949	85
			calmodulin-binding
			protein
20	508	L22557	Rattus norvegicus	2363	92
			calmodulin-binding
			protein
21	515	AK002158	Homo sapiens unnamed	1588	99
			protein product
22	578	AL080076	Homo sapiens	107	30
			hypothetical protein
23	588	AJ251516	Mus musculus cysteine	1460	99
			and histidine-rich
			protein
24	591	AL117551	Homo sapiens	1773	100
			hypothetical protein
25	593	AB033076	Homo sapiens KIAA1250	6286	100
			protein
26	594	AK000625	Homo sapiens unnamed	721	100
			protein product
27	619	AF161420	Homo sapiens HSPC302	2623	97
28	620	AL117477	Homo sapiens	2551	100
			hypothetical protein
29	654	AK001782	Homo sapiens unnamed	1161	100
			protein product
30	692	D25217	Homo sapiens KIAA0027	1911	100
			protein
31	753	AB041581	Mus musculus unnamed	1758	95
			protein product
32	758	X03414	Drosophila	316	45
			melanogaster Kr
			polypeptide
33	787	AF151079	Homo sapiens HSPC245	643	100
34	833	AK000643	Homo sapiens unnamed	614	53
			protein product
35	838	AB029022	Homo sapiens KIAA1099	1095	61
			protein
36	870	AF213465	Homo sapiens dual	2016	100
			oxidase
37	891	AF181562	Homo sapiens proSAAS	1319	100
38	891	AF181562	Homo sapiens proSAAS	1024	99
39	921	AB020671	Homo sapiens KIAA0864	5438	99
			protein
40	924	AB033051	Homo sapiens KIAA1225	4438	100
			protein
41	932	AB011105	Homo sapiens KIAA0533	8255	100
			protein
42	942	AB032983	Homo sapiens KIAA1157	2231	100
			protein
43	958	AF139077	Homo sapiens M5-14	1463	98
44	968	AK001366	Homo sapiens unnamed	2940	97
			protein product
45	992	AF198454	Homo sapiens	3927	100
			epithelial protein
			lost in neoplasm beta
46	1025	AK001753	Homo sapiens unnamed	217	68
			protein product
47	1074	AF169017	Homo sapiens	2717	98
			formiminotransferase
			cyclodeaminase
48	1104	A95106	unidentified RED ALPHA	1202	99
49	1114	AL137479	Homo sapiens	1942	100
			hypothetical protein
50	1144	AF072372	Mus musculus lysosomal	3388	97
			trafficking regulator
			2
51	1262	M14912	Homo sapiens pol	132	86
52	1318	AF090934	Homo sapiens PRO0518	382	100
53	1319	X66363	Homo sapiens	2499	100
			serine/threonine
			protein kinase
54	1328	AF072758	Mus musculus fatty	2097	87
			acid transport protein
			3; FATP3
55	1436	AB014514	Homo sapiens KIAA0614	8406	100
			protein
56	1464	AE003453	Drosophila	654	51
			melanogaster CG10509
			gene product
57	1584	AB033076	Homo sapiens KIAA1250	6286	100
			protein
58	1617	AB033067	Homo sapiens KIAA1241	4229	99
			protein
59	1724	D88585	Chlorocebus aethiops	401	36
			hepatitis A virus
			receptor
60	1728	AF208845	Homo sapiens BM-003	1375	99
61	1772	AB015427	Homo sapiens zinc	3934	100
			finger protein 219
62	1809	X57821	Homo sapiens	797	76
			immunoglobulin lambda
			light chain
63	1868	AF043695	Caenorhabditis elegans	555	43
			Similar to
			mitochondrial carrier
			protein
64	1898	AB033039	Hotno sapiens KIAA1213	2438	100
			protein
65	1926	AK000279	Homo sapiens unnamed	3271	99
			protein product
66	1965	AF178432	Homo sapiens SH3	3700	100
			protein
67	1967	AB033099	Homo sapiens KIAA1273	3082	99
			protein
68	1995	AF181721	Homo sapiens RU2S	2254	100
69	2005	AL133093	Homo sapiens	2241	100
			hypothetical protein
70	2027	U48238	Mus musculus zinc	749	63
			finger protein neuro-
			d4
71	2055	AL133105	Homo sapiens	1783	99
			hypothetical protein
72	2103	AB032958	Homo sapiens KIAA1132	9116	100
			protein
73	2106	AE003528	Drosophila	472	25
			melanogaster CG5018
			gene product
74	2166	AK001713	Homo sapiens unnamed	5323	99
			protein product
75	2175	AB010266	Mus musculus tenascin-	10246	64
			X
76	2176	AE003746	Drosophila	363	40
			melanogaster CG5986
			gene product
77	2194	AL163206	Homo sapiens protein	1944	100
			with homology to
			KIAA0790
78	2236	AB033020	Homo sapiens KIAA1194	2918	99
			protein
79	2250	AL122081	Homo sapiens	1930	100
			hypothetical protein
80	2300	AL133572	Homo sapiens	3303	100
			hypothetical protein
81	2323	AB033107	Homo sapiens KIAA1281	3228	100
			protein
82	2340	AB030183	Mus musculus contains	380	42
			transmembrane (TM)
			region
83	2371	Z18361	Ovis aries	184	32
			trichohyalin
84	2399	AJ010045	Mus musculus guanine	1470	58
			nucleotide-exchange
			factor
85	2411	AF176529	Mus musculus F-box	2072	94
			protein FBX13
86	2428	AF210842	Homo sapiens HARP	4880	100
87	2430	AL031658	Homo sapiens	776	98
			dJ310013.7 (novel
			protein similar to H.
			roretzi HRPET-3)
88	2439	X57398	Homo sapiens pm5	6131	99
			protein
89	2447	AE003779	Drosophiia	1670	62
			melanogaster CG2118
			gene product
90	2461	AL122097	Homo sapiens	3213	99
			hypothetical protein
91	2487	AE003801	Drosophila	247	38
			melanogaster CG14490
			gene product
92	2492	AB033072	Homo sapiens KIAA1246	4087	99
			protein
93	2512	AB033103	Homo sapiens KIAA1277	5252	99
			protein
94	2564	AF117946	Homo sapiens Link	2363	100
			guanine nucleotide
			exchange factor II
95	2678	AL133087	Homo sapiens	4159	99
			hypothetical protein
96	2816	AK001529	Homo sapiens unnamed	1420	99
			protein product
97	2818	AL137530	Homo sapiens	433	94
			hypothetical protein
98	2819	AB028942	Homo sapiens KIAA1019	7437	98
			protein
99	2943	AF189722	Homo sapiens PDZ-	1688	99
			binding kinase
100	3137	AE003450	Drosophila	681	48
			melanogaster CG2221
			gene product
101	3137	AE003450	Drosophila	716	38
			melanogaster CG2221
			gene product
102	3160	AK000708	Homo sapiens unnamed	1103	99
			protein product
103	3323	Y07829	Homo sapiens RING	2201	99
			finger protein
104	3360	AB007931	Homo sapiens KIAA0462	11741	99
			protein
105	3362	U41387	Homo sapiens Gu	4021	99
			protein
106	3417	AF023674	Homo sapiens	3783	100
			nephrocystin
107	3418	AF146760	Homo sapiens septin 2-	2284	100
			like cell division
			control protein
108	3442	Z66524	Caenorhabditis elegans	1190	48
			Homology with Squid
			retinal-binding
			protein (PIR Acc. No.
			A53057) ˜cDNA EST
			yk463d10.3 comes from
			this gene˜cDNA EST
			yk663h12.3 comes from
			this gene
109	3442	Z66524	Caenorhabditis elegans	848	42
			Homology with Squid
			retinal-binding
			protein (PIR Acc. No.
			A53057) ˜cDNA EST
			yk463d10.3 comes from
			this gene˜cDNA EST
			yk663h12.3 comes from
			this gene
110	3444	M26576	Homo sapiens alpha-1	9412	99
			type IV collagen
111	3855	AF113536	Homo sapiens MO25	1381	81
			protein
112	3863	AJ271385	Homo sapiens UDP-N-	733	46
			acetyl-alpha-D-
			galactosamine:polypeptide N-
			acetylgalactosaminyl
			transferase 8
113	4090	AF105228	Bos taurus tuftelin	285	33
114	4105	U32614	Mus musculus SOX6	2855	96
115	4142	X14971	Mus musculus alpha-	4897	98
			adaptin (A) (AA 1-977)
116	4142	X53773	Rattus norvegicus	3979	82
			alpha-c large chain
			(AA 1-938)
117	4149	AF034746	Mus musculus LNXp70	2922	88
118	4196	AC006551	Arabidopsis thaliana	214	34
			Hypothetical protein
119	4202	AF229032	Mus musculus piL	2077	93
120	4274	AF056035	Rattus norvegicus s-	2662	85
			nexilin
121	4304	AK000080	Homo sapiens unnamed	3037	99
			protein product
122	4306	D88158	Sus scrofa cytochrome	474	47
			b561
123	4311	AF161445	Homo sapiens HSPC327	1606	100
124	4321	AL133112	Homo sapiens	1861	100
			hypothetical protein
125	4323	AL137432	Homo sapiens	3002	100
			hypothetical protein
126	4332	AF186461	Rattus norvegicus ring	204	22
			finger protein Fxy
127	4488	AE003749	Drosophila	422	33
			melanogaster CG13644
			gene product
128	4588	D87438	Homo sapiens Similar	4069	100
			to a C. elegans protein
			in cosmid C14H10
129	5569	D87442	Homo sapiens KIAA0253	3682	100
130	5573	Z15005	Homo sapiens CENP-E	13305	99
131	5577	M59216	Homo sapiens gamma-	2477	100
			aminobutyric acid
			receptor beta-1
			subunit
132	5579	D31884	Homo sapiens KIAA0063	518	55
133	5582	AF188706	Homo sapiens g20	188	49
			protein
134	5583	AB029030	Homo sapiens KIAA1107	6581	99
			protein
135	5584	D87446	Homo sapiens Similar	9196	99
			to a C. elegans protein
			encoded in cosmid
			C27F2 (U40419)
136	5585	AF047663	Caenorhabditis elegans	22S	37
			W09G12.7 gene product
137	5591	AC002398	Homo sapiens F25965_1	1018	100
138	5593	AB023215	Homo sapiens KIAA0998	6323	99
			protein
139	5594	AF223408	Homo sapiens B99	3686	99
140	5594	AF223408	Homo sapiens B99	2878	88
141	5598	D83781	Homo sapiens the	6859	99
			KIAA0197 gene is
			expressed
			ubiquitously.; the
			KIAA0197 protein has
			histidine acid
			phosphatase signature
			at amino acid
			positions 1047-1061.
142	5602	U53450	Rattus norvegicus Jun	196	49
			dimerization protein 1
			JDP-1
143	5605	AL117233	Homo sapiens	3564	99
			hypothetical protein
144	5608	U38253	Rattus norvegicus	1203	89
			initiation factor eIF-
			2B gamma subunit
145	5617	AE003538	Drosophila	354	44
			melanogaster CG10191
			gene product
146	5620	AB020694	Homo sapiens KIAA0887	2328	100
			protein
147	5622	AB029025	Homo sapiens KIAA1102	4394	100
			protein
148	5623	AL137255	Homo sapiens	2636	100
			hypothetical protein
149	5624	AB018289	Homo sapiens KIAA0746	5223	99
			protein
150	5625	D38549	Homo sapiens ha1025 is	6533	99
			new
151	5627	AF241230	Homo sapiens TAK1-	3656	100
			binding protein 2
152	5628	AK000759	Homo sapiens unnamed	3306	100
			protein product
153	5630	AL117665	Homo sapiens	6463	100
			hypothetical protein
154	5632	AF161544	Homo sapiens HSPC059	434	77
155	5640	AJ238248	Homo sapiens centaurin	3986	99
			beta2
156	5641	AB007929	Homo sapiens KIAA0460	4781	99
			protein
157	5643	AF161381	Homo sapiens HSPC263	1404	100
158	5647	AF223468	Homo sapiens AD021	1314	100
			protein
159	5649	AF203343	Mus musculus RIBP	115	39
160	5658	X57527	Homo sapiens alpha	4166	99
			1 (VIII) collagen
161	5659	Y19062	Homo sapiens 39k3	2475	100
			protein
162	5667	AK000566	Homo sapiens unnamed	1053	100
			protein product
163	5672	AL021918	Homo sapiens b34I8.1	4184	100
			(Kruppel related Zinc
			Finger protein 184)
164	5674	AB020706	Homo sapiens KIAA0899	4732	100
			protein
165	5678	AB040915	Homo sapiens KIAA1482	2828	99
			protein
166	5680	AE001448	Helicobacter pylori	698	37
			J99 THREONINE
			SYNTHASE
167	5684	AF226614	Homo sapiens	2929	100
			ferroportin1
168	5686	Z93241	Homo sapiens	513	96
			dJ222E13.1 (novel
			protein with some
			similarity to
			Drosophila KRAKEN)
169	5694	AF036977	Homo sapiens unknown	1812	100
170	5698	AK001746	Homo sapiens unnamed	141	45
			protein product
171	5699	AF108843	Homo sapiens env	320	47
			protein
172	5712	AF069781	Drosophila	653	43
			melanogaster Bem46-
			like protein
173	5719	U95098	Xenopus laevis mitotic	1200	70
			phosphoprotein 44
174	5720	X70944	Homo sapiens PTB-	3883	100
			associated splicing
			factor
175	5727	AE003741	Drosophila	456	44
			melanogaster CG13832
			gene product
176	5730	AF195833	Mus musculus cell	2693	93
			adhesion molecule
			nectin-3 alpha
177	5734	AJ249732	Homo sapiens G8	669	100
			protein
178	5738	AF208861	Homo sapiens BM-019	1629	100
179	5739	L09708	Homo sapiens	4022	100
			complement component
			C2
180	5740	AF156961	Homo sapiens gag	106	47
181	5744	X66285	Mus musculus HC1 ORF	115	44
182	5748	D00189	Rattus norvegicus	5227	99
			Na+, K+-ATPase alpha-
			subunit
183	5749	U10185	Xenopus laevis XPMC2	1020	53
			protein
184	5750	AB019038	Homo sapiens beta-1,4	781	77
			mannosyltransferase
185	5750	AB019038	Homo sapiens beta-1,4	1347	100
			mannosyltransferase
186	5750	AB019038	Homo sapiens beta-1,4	1520	99
			mannosyltransferase
187	5761	X84908	Homo sapiens	5729	99
			phosphorylase kinase
188	5762	X52851	Homo sapiens	650	76
			peptidylprolyl
			isomerase
189	5767	AJ245671	Homo sapiens	3064	100
			hypothetical protein
190	5773	AC004447	Homo sapiens KIAA0365	4963	99
191	5783	U04706	Bos taurus 50 kDa	1749	78
			protein
192	5784	AF092207	Rattus norvegicus	1180	84
			unknown
193	5788	AK001934	Homo sapiens unnamed	1368	100
			protein product
194	5798	AK000284	Homo sapiens unnamed	3385	97
			protein product
195	5802	AF247042	Homo sapiens tandem	2186	99
			pore domain potassium
			channel TRAAK
196	5807	AF114494	Homo sapiens putative	1284	99
			tyrosine phosphatase
197	5818	AE000995	Archaeoglobus fulgidus	153	20
			chromosome segregation
			protein (smc1)
198	5819	AF062249	Homo sapiens	605	97
			immunoglobulin heavy
			chain variable region
199	5827	AJ223830	Rattus norvegicus ARE1	2950	98
200	5828	AL133027	Homo sapiens	1224	84
			hypothetical protein
201	5842	D87684	Homo sapiens KIAA0242	2566	100
			protein
202	5853	AL050318	Homo sapiens	524	79
			dJ977B1.3.1 (novel
			protein similar to
			putative RAB5-
			interacting protein
			(isoform 1))
203	5861	D49387	Homo sapiens NADP	1616	100
			dependent leukotriene
			b4 12-
			hydroxydehydrogenase
204	5864	AL050022	Homo sapiens	330	34
			hypothetical protein
205	5865	AL050267	Homo sapiens	3325	99
			hypothetical protein
206	5871	AL137300	Homo sapiens	1056	98
			hypothetical protein
207	5873	AK001480	Homo sapiens unnamed	1562	99
			protein product
208	5873	AK001480	Homo sapiens unnamed	1082	98
			protein product
209	5875	X12966	Homo sapiens 3-	1972	100
			oxoacyl-CoA thiolase
			propeptide (424 AA)
210	5878	Y09267	Homo sapiens flavin-	2486	100
			containing
			monooxygenase 2
211	5879	Z11773	Homo sapiens SRE-ZBP	2201	99
212	5880	D84224	Homo sapiens methionyl	4741	99
			tRNA synthetase
213	5880	D84224	Homo sapiens methionyl	3887	99
			tRNA synthetase
214	5880	D84224	Homo sapiens methionyl	2933	96
			tRNA synthetase
215	5880	D84224	Homo sapiens methionyl	4529	99
			tRNA synthetase
216	5885	J03244	Bos taurus H+ ATPase	848	77
			31 kDa subunit (EC
			3.6.1.3)
217	5895	AK001589	Homo sapiens unnamed	2313	100
			protein product
218	5898	AL117615	Homo sapiens	3174	99
			hypothetical protein
219	5902	AE003735	Drosophila	436	58
			melanogaster CG6353
			gene product
220	5904	A06669	synthetic construct	2070	99
			preTGF-beta1
221	5918	AE003487	Drosophila	238	26
			melanogaster CG1905
			gene product
222	5921	AL110243	Homo sapiens	2275	100
			hypothetical protein
223	5927	X60271	Mus musculus c-rel	2264	74
224	5932	AK001475	Homo sapiens unnamed	3025	100
			protein product
225	5939	AF131851	Homo sapiens Unknown	262	44
226	5945	AB002320	Homo sapiens KIAA0322	8183	100
227	5946	AE003518	Drosophila	135	22
			melanogaster CG6836
			gene product
228	5947	AF119855	Homo sapiens PRO1847	265	67
229	5956	M17236	Homo sapiens MHC HLA-	1332	100
			DQ alpha precursor
230	5967	AK001345	Homo sapiens unnamed	1453	99
			protein product
231	5968	M28515	Mus musculus zinc	225	28
			finger protein mfg3
			mRNA (put.); putative
232	5975	AB037730	Homo sapiens KIAA1309	515	44
			protein
233	5977	AE003464	Drosophila	610	44
			melanogaster CG11414
			gene product
234	5978	M12140	Homo sapiens pol gene	117	50
			protein; Xxx
235	5979	U79267	Homo sapiens unknown	225	56
236	5980	X56681	Homo sapiens junD	373	88
			protein
237	5988	AB023151	Homo sapiens KIAA0934	7099	100
			protein
238	5989	AL109839	Homo sapiens	877	100
			dJ1069P2.3.1 (novel
			PABPC1 (poly(A) -
			binding protein,
			cytoplasmic 1)
			(PABPL1) like protein
			(putative isoform 1))
239	5991	AE003583	Drosophila	289	42
			melanogaster
			BcDNA:GH09817 gene
			product
240	5997	AF052723	Feline leukemia virus	1547	44
			gag-pol precursor
			polyprotein gPr80
241	5998	AF161472	Homo sapiens HSPC123	439	45
242	6003	AK000360	Homo sapiens unnamed	796	100
			protein product
243	6004	U09848	Homo sapiens zinc	1738	100
			finger protein
244	6013	U19177	Homo sapiens Hin-2	55	46
245	6028	AF155113	Homo sapiens NY-REN-55	3603	93
			antigen
246	6028	AF155113	Homo sapiens NY-REN-55	3951	99
			antigen
247	6029	AL032821	Homo sapiens dJ55C23.1	1821	98
			(vanin 1)
248	6031	M69181	Homo sapiens non-	7350	99
			muscle myosin B
249	6031	M69181	Homo sapiens non-	7311	98
			muscle myosin B
250	6032	X61280	Oryza sativa	143	38
			hydroxyproline-rich
			glycoprotein
251	6037	AB002330	Homo sapiens KIAA0332	5362	100
252	6037	AB002330	Homo sapiens KIAA0332	4897	97
253	6043	X06745	Homo sapiens DNA	7619	99
			polymerase alpha-
			subunit (AA 1-1462)
254	6044	AF252292	Homo sapiens PAR6C	1342	100
255	6046	D86984	Homo sapiens similar	2446	100
			to yeast adenylate
			cyclase (S56776)
256	6048	AF165124	Homo sapiens gamma-	2499	99
			aminobutyric acid A
			receptor gamma 2
257	6049	AF110267	Rattus norvegicus	2088	89
			golgi stacking protein
			homolog GRASP55
258	6051	U82319	Homo sapiens novel ORF	342	100
259	6053	Y00816	Homo sapiens CR1	11396	99
			precursor protein
260	6060	AJ223948	Homo sapiens RNA	6608	99
			helicase
261	6063	Y08612	Homo sapiens 88 kDa	3874	99
			nuclear pore complex
			protein
262	6066	AB014597	Homo sapiens KIAA0697	5060	100
			protein
263	6067	AF129756	Homo sapiens BAT4	1873	98
264	6068	AF131775	Homo sapiens Unknown	1929	99
265	6073	AJ250865	Homo sapiens TESS 2	2348	100
266	6076	Z98885	Homo sapiens dJ522J7.2	5588	100
			(bromodomain-
			containing 1 (similar
			to peregrin, BR140))
267	6076	Z98885	Homo sapiens dJ522J7.2	4167	100
			(bromodomain-
			containing 1 (similar
			to peregrin, BR140))
268	6077	L76571	Homo sapiens nuclear	1355	100
			hormone receptor
269	6079	AF091622	Homo sapiens PHD	9054	100
			finger protein 3
270	6082	X56807	Homo sapiens	4443	100
			desmocollin type 2a
271	6087	AC002464	Homo sapiens organic	1542	99
			cation transporter;
			50% similarity to
			JC4884 (PID:g2143892)
272	6088	AL050272	Homo sapiens	697	99
			hypothetical protein
273	6091	AL022329	Homo sapiens	3653	100
			bK407F11.2
			(adrenergic, beta,
			receptor kinase 2)
274	6094	AK000833	Homo sapiens unnamed	2001	98
			protein product
275	6101	AJ245600	Homo sapiens	2616	99
			hypothetical protein
276	6103	AB041810	Mus musculus unnamed	1468	91
			protein product
277	6104	L36531	Homo sapiens integrin	5386	99
			alpha 8 subunit
278	6108	AL117646	Homo sapiens	1491	100
			hypothetical protein
279	6112	AF218584	Homo sapiens GGA1	3265	100
280	6121	Y13115	Homo sapiens	5071	99
			serine/threonine
			protein kinase
281	6125	AB018319	Homo sapiens KIAA0776	3960	99
			protein
282	6126	AL034452	Homo sapiens	1979	100
			dJ682J15.1 (novel
			Collagen triple helix
			repeat containing
			protein)
283	6128	Y14494	Homo sapiens aralar1	3465	99
284	6129	AJ001981	Homo sapiens OXA1L	2603	100
285	6133	A58799	unidentified unnamed	3069	100
			protein product
286	6133	A58799	unidentified unnamed	2464	100
			protein product
287	6135	AF163572	Homo sapiens Forssman	1865	99
			glycolipid synthetase
288	6139	AF161503	Homo sapiens HSPC154	1261	97
289	6141	AB011125	Homo sapiens KIAA0553	5754	100
			protein
290	6145	AJ250014	Homo sapiens Familial	3655	99
			Cylindromatosis Gene
291	6146	D25217	Homo sapiens KIAA0027	361	94
			protein
292	6148	X85786	Homo sapiens binding	3203	100
			regulatory factor
293	6149	Y08319	Homo sapiens kinesin-2	3487	99
294	6149	D12644	Mus musculus KIF2	3609	97
			protein
295	6153	U28789	Mus musculus PACT	5936	89
296	6159	AL137515	Homo sapiens	1687	100
			hypothetical protein
297	6164	AB020705	Homo sapiens KIAA0898	5017	100
			protein
298	6167	Y00062	Homo sapiens precursor	3440	99
			polypeptide (AA-23 to
			1120)
299	6172	AB007941	Homo sapiens KIAA0472	1925	99
			protein
300	6173	X98248	Homo sapiens sortilin	4403	99
301	6190	X61100	Homo sapiens 75 kDa	3734	99
			subunit NADH
			dehydrogenase
			precursor
302	6194	S58544	Homo sapiens 75 kDa	2125	99
			infertility-related
			sperm protein
303	6196	AL110265	Homo sapiens	744	100
			hypothetical protein
304	6197	X14968	Homo sapiens RII-alpha	2079	100
			subunit (AA 1-404)
305	6198	AL050283	Homo sapiens	1983	100
			hypothetical protein
306	6198	AL050283	Homo sapiens	1694	100
			hypothetical protein
307	6205	AJ011863	Homo sapiens homeobox	3841	99
			protein LSX
308	6214	AF098786	Homo sapiens 17 beta-	1754	100
			hydroxysteroid
			dehydrogenase type VII
309	6215	AL034555	Homo sapiens	4273	100
			dJ134O19.3 (zinc
			finger protein 151
			(pHZ-67))
310	6219	AB011167	Homo sapiens KIAA0595	7678	98
			protein
311	6226	U39205	Saccharomyces	277	29
			cerevisiae Lpe10p
312	6229	AF041429	Homo sapiens pRGR1	823	99
313	6234	X66357	Homo sapiens	1589	100
			serine/threonine
			protein kinase
314	6237	Y11284	Homo sapiens AFX1	2571	98
315	6238	AB004884	Homo sapiens PKU-alpha	3718	99
316	6239	AJ002303	Homo sapiens	1020	100
			synaptogyrin 1c
317	6239	AJ002304	Homo sapiens	1002	100
			synaptogyrin 1b
318	6239	AJ002303	Homo sapiens	933	94
			synaptogyrin 1c
319	6240	D87682	Homo sapiens similar	2676	100
			to a C. elegans protein
			encoded in cosmid
			T26A5.
320	6244	M14660	Homo sapiens ISG-K54	2473	99
321	6245	X06661	Homo sapiens calbindin	1358	100
			(AA 1-261)
322	6250	AF119900	Homo sapiens PR02822	185	76
323	6252	AB014527	Homo sapiens KIAA0627	6478	99
			protein
324	6252	AB014527	Homo sapiens KIAA0627	6372	98
			protein
325	6256	X86691	Homo sapiens Mi-2	10110	99
			protein
326	6260	AE003628	Drosophila	985	57
			melanogaster CG7475
			gene product
327	6261	AF236061	Oryctolagus cuniculus	3830	91
			RING-finger binding
			protein
328	6264	AB018327	Homo sapiens KIAA0784	5708	100
			protein
329	6265	AB018314	Homo sapiens KIAA0771	4949	100
			protein
330	6266	AB002318	Homo sapiens KIAA0320	4639	99
331	6270	X14766	Homo sapiens GABA-A	2388	99
			receptor alpha 1
			subunit
332	6271	AB023177	Homo sapiens KIAA0960	7294	99
			protein
333	6272	AB032957	Homo sapiens KIAA1131	8443	100
			protein
334	6274	AF007155	Homo sapiens unknown	187	61
335	6276	Z34975	Homo sapiens 1d1Cp	3733	100
336	6281	AL050306	Homo sapiens dJ475B7.2	3796	100
			(novel protein)
337	6281	AL050306	Homo sapiens dJ475B7.2	1942	99
			(novel protein)
338	6288	AB014566	Homo sapiens KIAA0666	5541	100
			protein
339	6292	AB018353	Homo sapiens KIAA0810	4246	100
			protein
340	6294	Z21966	Homo sapiens mPOU	1529	100
			homeobox protein
341	6299	AL022395	Homo sapiens	3287	100
			dJ273N12.1 (PUTATIVE
			protein based on EST
			matches)
342	6299	AL022395	Homo sapiens	2403	83
			dJ273N12.1 (PUTATIVE
			protein based on EST
			matches)
343	6312	AL096713	Homo sapiens	7599	99
			hypothetical protein
344	6312	AF182316	Homo sapiens myoferlin	6232	99
345	6312	AL096713	Homo sapiens	6120	99
			hypothetical protein
346	6322	AK000218	Homo sapiens unnamed	1163	99
			protein product
347	6324	D42046	Homo sapiens The	5568	100
			ha3631 gene product is
			related to
			S. cerevisiae protein
			encoded in chromosome
			VIII.
348	6328	AB023624	Rattus norvegicus SCOP	4792	92
349	6329	X59303	Homo sapiens valyl-	3393	99
			tRNA synthetase
350	6331	AC004142	Homo sapiens similar	3676	100
			to murine leucine-rich
			repeat protein;
			possible role in
			neural development by
			protein-protein
			interactions; 93%
			similarity to D49802
			(PID:g1369906)
351	6333	AC009991	Arabidopsis thaliana	609	51
			unknown protein
352	6334	AB018271	Homo sapiens KIAA0728	4316	98
			protein
353	6337	AB002318	Homo sapiens KIAA0320	4639	99
354	6339	AB039371	Homo sapiens	2902	99
			mitochondrial ABC
			transporter 3
355	6346	AK002198	Homo sapiens unnamed	2570	99
			protein product
356	6348	AB033087	Homo sapiens KIAA1261	4094	99
			protein
357	6348	L14463	Rattus norvegicus	3619	92
			transducin
358	6350	AC005757	Homo sapiens R32611_1	2779	100
359	6351	S61069	Homo sapiens reverse	252	66
			transcriptase
			homolog = pol
			{retroviral element}
360	6355	AF271388	Homo sapiens CMP-N-	2273	100
			acetylneuraminic acid
			synthase
361	6362	X79066	Homo sapiens ERF-1	1783	100
362	6368	AF118566	Mus musculus	769	51
			hematopoietic zinc
			finger protein
363	6369	AB020710	Homo sapiens KIAA0903	4915	99
			protein
364	6371	AF143321	Homo sapiens unknown	661	65
365	6376	AF260011	Homo sapiens HSPC087-	8764	99
			KIAA0714
366	6379	583365	Homo sapiens putative	131	49
			Rab5-interacting
			protein {clone L1-94}
367	6380	AL021878	Homo sapiens	154	68
			dJ257I204
			(transcription factor
			20 (AR1) (KIAA0292)
			(isoform 2))
368	6381	D90734	Escherichia coli	628	100
			ORF_ID:o223#11
369	6392	M58378	Homo sapiens synapsin	3730	99
			I
370	6395	AF039697	Homo sapiens antigen	508	98
			NY-CO-31
371	6397	U09355	Oryctolagus cuniculus	2356	99
			protein phosphatase
			2A1 B gamma subunit
372	6400	AB002293	Homo sapiens KIAA0295	5054	100
373	6401	AC004774	Homo sapiens Dlx-5	1542	100
374	6411	X90530	Homo sapiens ragB	1926	99
375	6411	X90530	Homo sapiens ragB	1405	99
376	6411	X90530	Homo sapiens ragB	1590	85
377	6416	AL080157	Homo sapiens	2100	94
			hypothetical protein
378	6418	AE003628	Drosophila	659	49
			melanogaster CG5188
			gene product
379	6422	AB007884	Homo sapiens KIAA0424	2757	99
380	6423	AB018323	Homo sapiens KIAA0780	5631	100
			protein
381	6426	AF042713	Rattus norvegicus	1337	96
			neurexophilin 3
382	6427	AJ131891	Homo sapiens DNA	1451	100
			polymerase mu
383	6428	AF221712	Homo sapiens Smad- and	6705	100
			Olf-interacting zinc
			finger protein
384	6429	X83573	Homo sapiens ARSE	3184	99
385	6430	AJ243274	Homo sapiens AP-2rep	2078	99
			protein
386	6432	AL035608	Homo sapiens dJ479J7.1	1440	100
			(similar to
			CHONDROMODULIN-1)
387	6432	AL035608	Homo sapiens dJ479J7.1	1316	93
			(similar to
			CHONDROMODULIN-1)
388	6438	AK001444	Homo sapiens unnamed	943	100
			protein product
389	6441	AL022237	Homo sapiens	2030	100
			bK1191B2.3 (PUTATIVE
			novel Acyl Transferase
			similar to C. elegans
			C50D2.7) (isoform 1))
390	6446	AJ006266	Homo sapiens AND-1	5942	100
			protein
391	6454	AL110240	Homo sapiens	704	98
			hypothetical protein
392	6459	AL050149	Homo sapiens	2899	100
			hypothetical protein
393	6460	AL096772	Homo sapiens	7049	99
			dJ365O12.1 (KIAA0758
			protein)
394	6461	AB008376	Sus scrofa 17-kDa PKC-	689	91
			potentiated inhibitory
			protein of PP1
395	6467	M22334	Homo sapiens unknown	796	59
			protein
396	6468	AK002144	Homo sapiens unnamed	2719	100
			protein product
397	6487	AL117429	Homo sapiens	1077	100
			hypothetical protein
398	6491	AB027004	Homo sapiens protein	435	48
			phosphatase
399	6506	AL137013	Homo sapiens bA311P8.3	862	100
			(probable uracil
			phosphoribosyltranferase)
400	6513	AL080141	Homo sapiens	4793	99
			hypothetical protein
401	6514	AB035123	Mus musculus GD1	1696	93
			alpha/GT1a alpha/GQ1b
			alpha synthase
402	6519	K02882	Homo sapiens	2048	100
			immunoglobulin delta-
			chain
403	6521	X07979	Homo sapiens integrin	4347	99
			beta 1 subunit
			precursor
404	6532	AJ224819	Homo sapiens tumor	2149	99
			suppressor
405	6536	Y07595	Homo sapiens	2373	100
			transcription factor
			TFIIH
406	6543	D14479	Rattus norvegicus	1428	88
			calpain
407	6544	AF161341	Homo sapiens HSPC078	1097	98
408	6548	AF187318	Homo sapiens F-box	1607	100
			protein Fbx2
409	6551	AL050369	Homo sapiens	2495	99
			hypothetical protein
410	6551	AL050369	Homo sapiens	2135	99
			hypothetical protein
411	6552	AF003785	Drosophila	1211	56
			melanogaster CG12792
			gene product
412	6554	AF091083	Homo sapiens unknown	1514	100
413	6556	AK001708	Homo sapiens unnamed	2334	99
			protein product
414	6560	AE003602	Drosophila	462	38
			melanogaster CG2109
			gene product
415	6563	AB011139	Homo sapiens KIAA0567	4966	99
			protein
416	6564	AK001177	Homo sapiens unnamed	1933	100
			protein product
417	6567	D63484	Homo sapiens The	4951	99
			KIAA0150 gene product
			is novel.
418	6573	AB029012	Homo sapiens KIAA1089	5128	100
			protein
419	6575	AL035461	Homo sapiens	1562	98
			dJ967N21.6 (novel CDP-
			alcohol
			phosphatidyltransferase
			family member
			protein)
420	6577	AK001236	Homo sapiens unnamed	1676	99
			protein product
421	6593	AF079098	Homo sapiens arginine-	2733	99
			tRNA-protein
			transferase 1-lp;
			ATE1-lp
422	6595	AJ131712	Homo sapiens nucleolar	2793	100
			RNA-helicase
423	6599	AJ133115	Homo sapiens TSC-22-	2054	99
			like protein
424	6625	X98258	Homo sapiens M-phase	953	100
			phosphoprotein 9
425	6625	X98258	Homo sapiens M-phase	564	75
			phosphoprotein 9
426	6626	U97191	Caenorhabditis elegans	960	85
			strong similarity to
			the YPT1 sub-family of
			RAS proteins
427	6630	X76057	Homo sapiens	2191	100
			phosphomannose
			isomerase
428	6631	AE003559	Drosophila	650	31
			melanogaster CG8605
			gene product
429	6632	X97064	Homo sapiens Sec23	4034	99
			protein
430	6633	AF161401	Homo sapiens HSPC283	779	100
431	6634	AJ005642	Rattus rattus serine	717	48
			protease
432	6638	M19529	Sus scrofa follistatin	1906	98
			A
433	6641	AJ249457	Trichomonas vaginalis	183	28
			centrin, putative
434	6644	AC004410	Homo sapiens	2094	100
			fos39554_1
435	6646	AK000096	Homo sapiens unnamed	2157	99
			protein product
436	6648	AF252284	Homo sapiens	4005	100
			transcription
			specificity factor Sp1
437	6652	Z92825	Caenorhabditis elegans	541	43
			predicted using
			Genefinder˜cDNA EST
			yk315e12.3 comes from
			this gene˜cDNA EST
			yk315e12.5 comes from
			this gene˜cDNA EST
			yk605b12.3 comes from
			this gene
438	6654	D79205	Homo sapiens ribosomal	160	77
			protein L39
439	6657	AL031027	Unknown/	584	58
			prediction = (method: ““
			genefinder””,
			version: ““084””,
			score: ““67.72””) ˜/pred
			iction = (method
440	6658	S49657	Mus sp. mitochondrial	91	35
			capsule selenoprotein;
			MCS
441	6663	M26312	Oryctolagus cuniculus	82	30
			unknown protein
442	6664	L32162	Homo sapiens	574	80
			transcription factor
443	6668	AL050060	Homo sapiens	526	99
			hypothetical protein
444	6669	AF205936	Mus musculus ADP-	296	39
			ribosylation factor-
			like membrane-
			associated protein
445	6673	AK000387	Homo sapiens unnamed	1136	100
			protein product
446	6685	U38934	Gallus gallus histone	625	97
			H2A
447	6687	U76374	Mus musculus skm-B0P2	602	31
448	6689	X13403	Homo sapiens Oct-1	3626	100
			protein (AA 1-743)
449	6693	AB023139	Homo sapiens KIAA0922	4258	100
			protein
450	6698	AE003467	Drosophila	274	27
			melanogaster CG7047
			gene product
451	6699	AL049176	Homo sapiens dA141H5.1	1401	99
			(C-terminal part of a
			Chordin LIKE protein
			with von Willebrand
			factor type C domains)
452	6705	X92475	Homo sapiens ITBA1	1429	100
453	6711	Y16752	Homo sapiens	1422	99
			secretagogin
454	6713	X51416	Homo sapiens hormone	2641	97
			receptor hERR1 (AA 1-
			521)
455	6716	AJ006591	Homo sapiens cysteine-	1793	100
			rich protein
456	6725	A08695	Homo sapiens rap2	935	100
457	6726	Z12173	Homo sapiens N-	2970	100
			acetylglucosamine-6-
			sulphatase
458	6727	AL355092	Homo sapiens	924	98
			hypothetical protein
459	6730	AB007930	Homo sapiens KIAA0461	7164	100
			perotein
460	6730	AB007930	Homo sapiens KIAA0461	6960	99
			perotein
461	6730	AB007930	Homo sapiens KIAA0461	6018	89
			perotein
462	6732	D38491	Homo sapiens KIAA0117	1119	99
463	6733	AJ012590	Homo sapiens glucose	4155	99
			1-dehydrogenase
464	6737	AL080133	Homo sapiens	5677	100
			hypothetical protein
465	6745	Z75532	Caenorhabditis elegans	220	35
			similar to
			mitochrondrial carrier
			protein˜cDNA EST
			yk264h5.5 comes from
			this gene
466	6751	AF207829	Homo sapiens SCAN-	900	100
			related protein RAZ1
467	6754	AF061262	Mus musculus semaF	1316	83
			cytoplasmic domain
			associated protein 2
468	6758	AF220189	Homo sapiens	605	89
			uncharacterized
			hypothalamus protein
			HBEX2
469	6761	AL079292	Homo sapiens	4135	100
			hypothetical protein,
			similar to (AC007017)
			putative RNA helicase
			A
470	6765	Z22819	Mus musculus Rab24	1042	98
			protein
471	6768	Z97029	Homo sapiens	1548	99
			ribonuclease HI large
			subunit
472	6773	AB035384	Homo sapiens SRp25	962	94
			nuclear protein
473	6776	AF024631	Homo sapiens ANG2	2644	100
474	6796	AJ006710	Rattus norvegicus	4508	97
			phosphatidylinositol
			3-kinase
475	6798	AL137275	Homo sapiens	4310	100
			hypothetical protein
476	6823	V00638	bacteriophage lambda	600	100
			reading frame ea10
477	6825	AF049103	Homo sapiens	819	100
			Huntingtin interacting
			protein
478	6826	U50312	Caenorhabditis elegans	92	40
			strong similarity to
			the a portion of the
			triple-helical region
			of collagen alpha
			chain
479	6839	Z26317	Homo sapiens	4810	99
			desmoglein 2
480	6844	AF227899	Homo sapiens breast	4443	99
			carcinoma-associated
			antigen isoform I
481	6847	AF106037	Homo sapiens	4905	99
			adipocyte-derived
			leucine aminopeptidase
482	6849	U15155	Gallus gallus	372	37
			trypsinogen
483	6854	D86974	Homo sapiens KIAA0220	2870	99
484	6857	AF112201	Homo sapiens neuronal	1053	100
			protein NP25
485	6861	AF234765	Rattus norvegicus	958	64
			serine-arginine-rich
			splicing regulatory
			protein SRRP86
486	6873	AF117383	Homo sapiens placental	502	68
			protein 13; PP13
487	6875	AK002059	Homo sapiens unnamed	1665	100
			protein product
488	6877	AE003438	Drosophila	338	43
			melanogaster CG3184
			gene product
489	6880	AK000101	Homo sapiens unnamed	814	100
			protein product
490	6885	AK000609	Homo sapiens unnamed	1160	100
			protein product
491	6890	AB023201	Homo sapiens KIAA0984	3743	98
			protein
492	6890	AB023201	Homo sapiens KIAA0984	2361	97
			protein
493	6894	AB013885	Homo sapiens beta-	1494	100
			ureidopropionase
494	6901	AL096725	Homo sapiens	1901	100
			hypothetical protein
495	6904	AK001901	Homo sapiens unnamed	2212	99
			protein product
496	6907	AF226077	Homo sapiens CHRAC17	724	99
497	6914	AE003762	Drosophila	646	75
			melanogaster CG5590
			gene product
498	6917	Z73497	Homo sapiens cU240C2.2	324	100
			(Core histone
			H2A/H2B/H3/H4)
499	6923	Z83246	Caenorhabditis elegans	891	60
			predicted using
			Genefinder˜cDNA EST
			EMBL:M79771 comes from
			this gene
500	6929	X16282	Homo sapiens zinc	1109	99
			finger protein (217
			AA) (1 is 2nd base in
			codon)
501	6931	Z92539	Mycobacterium	300	36
			tuberculosis pth
502	6935	M62324	Homo sapiens modulator	2902	96
			recognition factor I
503	6940	AC024762	Caenorhabditis elegans	434	43
			Hypothetical protein
			Y38F2AL.f
504	6945	AL117555	Homo sapiens	321	94
			hypothetical protein
505	6946	AC005328	Homo sapiens R26660_2,	865	97
			partial CDS
506	6947	AF151075	Homo sapiens HSPC241	686	98
507	6949	L34807	Musca domestica	174	21
			transposase
508	6959	AJ271091	Homo sapiens B-ind1	494	42
			protein
509	6960	AK001348	Homo sapiens unnamed	1853	99
			protein product
510	6962	AJ006692	Homo sapiens ultra	693	74
			high sulfer keratin
511	6963	U23037	Oryctolagus cuniculus	3406	90
			eIF-2Bepsilon
512	6967	AL136571	Homo sapiens	413	58
			hypothetical protein
513	6983	AF151800	Homo sapiens CGI-41	84	35
			protein
514	6988	AF198100	Fowlpox virus ORF	567	54
			FPV114 HAL3 domain
515	6996	AL137764	Homo sapiens	2162	100
			hypothetical protein
516	7003	AB011792	Homo sapiens	274	35
			extracellular matrix
			protein
517	7016	AB011542	Homo sapiens MEGF9	2097	100
518	7017	AL096744	Homo sapiens	231	68
			hypothetical protein
519	7025	AF119664	Homo sapiens	1574	100
			transcriptional
			regulator protein
			HCNGP
520	7025	AF119664	Homo sapiens	1144	89
			transcriptional
			regulator protein
			HCNGP
521	7025	AF119664	Homo sapiens	1448	94
			transcriptional
			regulator protein
			HCNGP
522	7050	X12517	Homo sapiens C protein	918	100
			(AA 1-159)
523	7051	AL079277	Homo sapiens	1294	100
			hypothetical protein,
			similar to (U32865)
			linotte protein
524	7055	AF067730	Homo sapiens TLS-	631	57
			associated protein
			TASR-2
525	7060	U27831	Homo sapiens striatum-	2840	98
			enriched phosphatase
526	7064	L26288	Rattus norvegicus CaM-	1416	82
			like protein kinase
527	7067	AL032684	Schizosaccharomyces	300	37
			pombe hypothetical
			protein
528	7071	AL050028	Homo sapiens	671	100
			hypothetical protein
529	7072	X78444	Rattus norvegicus	450	73
			ribosomal protein L22
530	7073	U27838	Mus musculus glycosyl-	3305	96
			phosphatidyl-inositol-
			anchored protein
			homolog
531	7076	AB037807	Homo sapiens KIAA1386	4001	99
			protein
532	7088	AJ276504	Mus musculus	1705	85
			phosphorylated adaptor
			for RNA export
533	7089	AB033079	Homo sapiens KIAA1253	2398	100
			protein
534	7091	U41315	Homo sapiens ZNF127-Xp	2458	93
535	7091	AF192784	Homo sapiens makorin 1	2062	97
536	7104	AE003704	Drosophila	510	44
			melanogaster CG3307
			gene product
537	7105	Z22968	Homo sapiens M130	6205	100
			antigen
538	7105	Z22971	Homo sapiens M130	6380	100
			antigen extracellular
			variant
539	7109	AL050225	Homo sapiens	1431	99
			hypothetical protein
540	7109	AL050225	Homo sapiens	932	99
			hypothetical protein
541	7119	Z46522	Drosophila subobscura	237	55
			bcn92
542	7120	AE003771	Drosophila	2185	68
			melanogaster CG1972
			gene product
543	7121	AL021546	Homo sapiens	593	100
			Cytochrome C Oxidase
			Polypeptide VIa-liver
			precursor (EC 1.9.3.1)
544	7126	L02956	Xenopus laevis	1664	87
			ribonucleoprotein
545	7127	AF201947	Homo sapiens MEK	616	100
			binding partner 1
546	7130	L31783	Mus musculus uridine	1266	92
			kinase
547	7131	AK001534	Homo sapiens unnamed	652	97
			protein product
548	7144	AE003834	Drosophila	485	57
			melanogaster CG8026
			gene product
549	7159	AF154108	Homo sapiens tumor	3559	99
			necrosis factor type 1
			receptor associated
			protein
550	7163	AE003066	Drosophila	251	34
			melanogaster CG13865
			gene product
551	7175	X57807	Homo sapiens	699	91
			immunoglobulin lambda
			light chain
552	7188	AL031673	Homo sapiens	4066	99
			dJ694B14.1 (PUTATIVE
			novel KRAB box protein
			with 18 C2H2 type Zinc
			finger domains)
553	7189	Y11652	Homo sapiens phosphate	238	100
			cyclase
554	7190	AF192968	Homo sapiens high-	3041	99
			glucose-regulated
			protein 8
555	7191	AB020648	Homo sapiens KIAA0841	3237	99
			protein
556	7203	AL031427	Homo sapiens	1608	100
			dJ167A19.1 (novel
			protein)
557	7204	AF151534	Homo sapiens core	1866	100
			histone macroH2A2.2
558	7208	AL021331	Homo sapiens	1129	100
			dJ366N23.1 (putative
			C. elegans UNC-93
			(protein 1, C46F11.1)
			LIKE protein)
559	7209	X14608	Homo sapiens	3579	100
			propionyl-COA
			carboxylase
560	7210	AL110249	Homo sapiens	4488	99
			hypothetical protein
561	7216	AC004982	Homo sapiens similar	2038	100
			to yeast hypothetical
			protein ybk4; similar
			to P38164
			(PID:g586461)
562	7221	AE003628	Drosophila	148	30
			melanogaster CG5676
			gene product
563	7230	AE003519	Drosophila	711	75
			melanogaster CG4108
			gene product
564	7237	X79417	Sus scrofa 40S	687	100
			ribosomal protein S12
565	7240	AB023203	Homo sapiens KIAA0986	7551	100
			protein
566	7245	AE003684	Drosophila	1106	51
			melanogaster CG8412
			gene product
567	7250	AL117662	Homo sapiens	1078	99
			hypothetical protein
568	7251	AB041261	Homo sapiens calcium-	2903	100
			independent
			phospholipase A2
569	7255	AK000812	Homo sapiens unnamed	1350	100
			protein product
570	7260	Y10936	Homo sapiens	1104	99
			hypothetical protein
571	7265	AK000444	Homo sapiens unnamed	2900	99
			protein product
572	7268	AK001798	Homo sapiens unnamed	1460	99
			protein product
573	7275	AL117635	Homo sapiens	929	99
			hypothetical protein
574	7279	M55531	Homo sapiens GLUT5	924	45
			protein
575	7283	AL117573	Homo sapiens	2907	99
			hypothetical protein
576	7283	AL117573	Homo sapiens	2457	97
			hypothetical protein
577	7287	AF237631	Homo sapiens	1798	100
			ubiquitous
			tropomodulin U-Tmod
578	7301	AF090929	Homo sapiens PRO0477p	653	99
579	7308	AL031228	Homo sapiens	3196	100
			dJ1033B10.2 (WD40
			protein BING4 (similar
			to S. cerevisiae
			YER082C, M. sexta
			MNG10 and C. elegans
			F28D1.1)
580	7308	AL031228	Homo sapiens	2825	96
			dJ1033B10.2 (WD40
			protein BING4 (similar
			to S. cerevisiae
			YER082C, M. sexta
			MNG10 and C. elegans
			F28D1.1)
581	7309	AF171102	Homo sapiens retinal	1302	95
			degeneration B beta
582	7319	AK001598	Homo sapiens unnamed	2775	100
			protein product
583	7320	AJ237946	Homo sapiens DEAD Box	2443	100
			Protein 5
584	7326	Z97184	Homo sapiens HKE2	624	100
585	7326	Z97184	Homo sapiens HKE2	409	98
586	7334	AJ245587	Homo sapiens Kruppel-	1942	100
			type zinc finger
587	7337	Z22820	Canis familiaris	995	98
			Rab22a protein
588	7339	X64701	Haloferax mediterranei	103	28
			gvpI
589	7344	L04733	Homo sapiens kinesin	1936	72
			light chain
590	7355	AB020681	Homo sapiens KIAA0874	3090	100
			protein
591	7363	M55542	Homo sapiens guanylate	2993	98
			binding protein
			isoform I
592	7363	M55542	Homo sapiens guanylate	2901	96
			binding protein
			isoform I
593	7365	U41857	Xenopus laevis WD-40	937	53
			motifs; up-regulated
			by thyroid hormone in
			tadpoles
594	7368	M26285	Xenopus laevis myc	82	28
			protein
595	7369	AB029150	Homo sapiens KRAB zinc	2196	100
			finger protein HFB101L
596	7372	AK000706	Homo sapiens unnamed	1641	100
			protein product
597	7373	AB041648	Mus musculus unnamed	625	100
			protein product
598	7374	AB032976	Homo sapiens KIAA1150	1929	100
			protein
599	7375	AB011182	Homo sapiens KIAA0610	3467	100
			protein
600	7381	AJ243721	Homo sapiens dTDP-4-	1710	100
			keto-6-deoxy-D-glucose
			4-reductase
601	7383	Z46676	Caenorhabditis elegans	312	40
			cDNA EST yk484g1.3
			comes from this
			gene˜cDNA EST
			yk484g1.5 comes from
			this gene
602	7387	L24804	Homo sapiens p23	350	43
603	7391	AK000453	Homo sapiens unnamed	1843	99
			protein product
604	7393	D50807	Bos taurus synaphin	146	35
605	7395	M23159	Cricetus cricetus	163	31
			DHFR-coamplified
			protein
606	7397	AB020684	Homo sapiens KIAA0877	3034	100
			protein
607	7399	AK002205	Homo sapiens unnamed	1331	97
			protein product
608	7405	AL096779	Homo sapiens	1544	100
			hypothetical protein
609	7406	AL161495	Arabidopsis thaliana	866	43
			putative WD-repeat
			protein
610	7406	AL161495	Arabidopsis thaliana	442	36
			putative WD-repeat
			protein
611	7409	U97001	Caenorhabditis elegans	605	52
			similar to
			Schizosaccharomyces
			pombe 4-
			nitrophenylphosphatase
			(PNPPASE) (GB:X62722,
			NID:g5005)
612	7410	X71978	Mus musculus Fif	1503	95
613	7411	AL117526	Homo sapiens	4375	99
			hypothetical protein
614	7417	AL031765	Unknown/	364	35
			prediction = (method: ““
			genefinder””,
			version: ““084””,
			score: ““31.96””) ˜/pred
			iction = (method
615	7418	AK001743	Homo sapiens unnamed	2248	99
			protein product
616	7421	AE003557	Drosophila	471	39
			melanogaster CG7388
			gene product
617	7422	AJ224326	Homo sapiens ribulose-	912	100
			5-phosphate-epimerase
618	7422	AE003840	Drosophila	363	60
			melanogaster CG1364
			gene product
619	7423	AB023191	Homo sapiens KIAA0974	2953	100
			protein
620	7424	AE003750	Drosophila	201	31
			melanogaster CG11839
			gene product
621	7426	AJ276485	Homo sapiens integral	1200	100
			membrane transporter
			protein
622	7427	AK000062	Homo sapiens unnamed	1390	63
			protein product
623	7428	AB026808	Mus musculus	2142	95
			synaptotagmin XI
624	7430	AB015345	Homo sapiens	2601	99
			HRIHFB2216
625	7435	X65724	Homo sapiens ORF2	498	100
626	7437	AE003474	Drosophila	489	43
			melanogaster CGT275
			gene product
627	7439	AK002204	Homo sapiens unnamed	1138	100
			protein product
628	7440	AK001675	Homo sapiens unnamed	1289	100
			protein product
629	7442	AC006978	Homo sapiens supported	501	100
			by human and rodent
			ESTs; match to
			AA454028
			(NID:g2167697),
			similar to AA9255224
			(NID:g4236415) and
			AA023712
			(NID:g1487627)
630	7450	AF129756	Homo sapiens G5c	273	100
631	7451	M23765	Rattus norvegicus	133	96
			alpha - tropomyosin
632	7452	Z80220	Caenorhabditis elegans	601	57
			Similarity to yeast
			protein TREMBL ID
			E246895)˜cDNA EST
			EMBL:T00018 comes from
			this gene˜cDNA EST
			EMBL:C13908 comes from
			this gene˜cDNA EST
			EMBL:C11656 comes from
			this gene˜cDNA EST
			yk234a5.3 comes from
			this gene˜cDNA EST
			yk234a5.5 comes from
			this gene˜cDNA EST
			yk590h6.3 comes from
			this gene
633	7454	AL117530	Homo sapiens	2121	99
			hypothetical protein
634	7457	AF055473	Homo sapiens GAGE-8	273	52
635	7459	AL050147	Homo sapiens	2847	100
			hypothetical protein
636	7461	AF143956	Mus musculus coronin-2	2300	93
637	7463	AK002072	Homo sapiens unnamed	1858	98
			protein product
638	7466	AF060076	Mus musculus	147	45
			polyhomeotic 2 protein
639	7469	Z98944	Schizosaccharomyces	159	44
			pombe hypothetical
			protein
640	7473	U66208	Ascaris suum AsSLR8.60	128	54
641	7481	AK000337	Homo sapiens unnamed	1319	62
			protein product
642	7482	U09410	Homo sapiens zinc	2483	99
			finger protein ZNF131
643	7482	U09410	Homo sapiens zinc	1853	99
			finger protein ZNF131
644	7483	AF068302	Homo sapiens	1356	66
			choline/ethanolamineph
			osphotransferase
645	7485	AK000427	Homo sapiens unnamed	1140	100
			protein product
646	7486	U54807	Rattus norvegicus GTP-	1167	97
			binding protein
647	7487	AF058807	Bos taurus GTP-binding	606	97
			protein rah
648	7491	AL050269	Homo sapiens	1066	99
			hypothetical protein
649	7492	AE003652	Drosophila	587	40
			melanogaster CG13284
			gene product
650	7494	AE003526	Drosophila	753	51
			melanogaster CG4098
			gene product
651	7498	AB033045	Homo sapiens KIAA1219	2674	99
			protein
652	7504	X61381	Rattus rattus	202	46
			interferon-induced
			protein
653	7508	D38169	Homo sapiens inositol	3278	100
			1,4,5-trisphosphate 3-
			kinase isoenzyme
654	7516	AL031432	Homo sapiens	893	100
			dJ465N24.2.1 (PUTATIVE
			novel protein)
			(isoform 1)
655	7518	U79275	Homo sapiens unknown	611	100
656	7519	AJ011306	Homosapiens guanine	2752	99
			nucleotide exchange
			factor (long isoform)
657	7521	AL355775	Arabidopsis thaliana	368	48
			putative protein
658	7529	AF116827	Homo sapiens unknown	3020	99
659	7532	AE003795	Drosophila	630	59
			melanogaster CG15120
			gene product
660	7533	AB031292	Mus musculus	130	31
			proteolipid protein 2
661	7535	U25801	Homo sapiens Tax1	852	98
			binding protein
662	7545	AF049523	Homo sapiens	1390	97
			huntingtin-interacting
			protein HYPA/FBP11
663	7546	AK001809	Homo sapiens unnamed	1040	100
			protein product
664	7552	AF028823	Homo sapiens Tax	581	100
			interaction protein 1
665	7554	AE003467	Drosophila	262	41
			melanogaster CG13880
			gene product
666	7567	U94991	Xenopus laevis	795	97
			transcription factor
			XLMO1
667	7569	S73775	Homo sapiens	2029	100
			calmitine;
			calsequestrine
668	7575	AE003579	Drosophila	1023	45
			melanogaster CG17593
			gene product
669	7576	AJ243191	Homo sapiens heat	827	96
			shock protein
670	7577	X65020	Bos taurus PSST	964	86
			subunit of the NADH:
			ubiquinone
			oxidoreductase complex
671	7579	AE003731	Drosophila	495	49
			melanogaster CG10877
			gene product
672	7582	Z30093	Homo sapiens basic	1576	99
			transcription factor
			2, 35 kD subunit
673	7587	AB030835	Homo sapiens contains	4697	99
			two glutamine rich
			domains, three zinc-
			finger domains, and
			matrin 3 homologous
			domain 3 (MH3)
674	7589	AB023222	Homo sapiens KIAA1005	5410	100
			protein
675	7597	AL022238	Homo sapiens	4048	99
			dJ1042K10.2 (supported
			by GENSCAN, FGENES and
			GENEWISE)
676	7597	AL022238	Homo sapiens	2321	99
			dJ1042K10.2 (supported
			by GENSCAN, FGENES and
			GENEWISE)
677	7609	AL117237	Homo sapiens	4820	99
			hypothetical protein
678	7609	AK000726	Homo sapiens unnamed	3767	96
			protein product
679	7609	AK000726	Homo sapiens unnamed	3227	92
			protein product
680	7613	AL023859	Schizosaccharomyces	172	42
			pombe trna-splicing
			endonuclease subunit
681	7623	AC005023	Homo sapiens match to	789	100
			EST AA361117
			(NID:g2013436)
682	7629	AC005253	Homo sapiens R26445_1	902	100
683	7630	AF151070	Homo sapiens HSPC236	951	98
684	7633	AF103801	Homo sapiens unknown	2555	100
685	7635	AC004000	Homo sapiens match to	388	100
			EST AA085966
			(NID:g1629547)
686	7638	AK001712	Homo sapiens unnamed	1586	99
			protein product
687	7639	M24103	Bos taurus translocase	1512	97
688	7646	D79990	Homo sapiens KIAA0168	899	60
689	7647	AF208844	Homo sapiens BM-002	428	100
690	7648	AL023496	Streptomyces	163	35
			coelicolor A3 (2)
			hypothetical protein
691	7658	AL031431	Homo sapiens	2058	100
			dJ462023.2 (novel
			protein)
692	7664	S45367	Canis familiaris	1949	100
			centractin
693	7664	S45367	Canis familiaris	1315	98
			centractin
694	7672	U88573	Homo sapiens NBR2	566	92
695	7674	D43950	Homo sapiens KIAA0098	2732	100
			protein
696	7675	AE003708	Drosophila	930	40
			melanogaster CG5038
			gene product
697	7676	AL080125	Homo sapiens	3002	100
			hypothetical protein
698	7681	AE003690	Drosophila	276	67
			melanogaster CG14701
			gene product
699	7688	AL080125	Homo sapiens	3181	100
			hypothetical protein
700	7693	Z14000	Homo sapiens RING1	2017	100
701	7694	AC013289	Arabidopsis thaliana	189	44
			hypothetical protein
702	7715	AB041607	Mus musculus unnamed	2345	94
			protein product
703	7716	AF251041	Homo sapiens SGC32445	535	70
			protein
704	7718	AE003427	Drosophila	527	51
			melanogaster CG10802
			gene product
705	7721	AC012329	Arabidopsis thaliana	690	38
			putative transporter
706	7723	X67250	Rattus norvegicus n-	1710	97
			chimaerin
707	7729	U05784	Rattus norvegicus	609	96
			light chain 3 subunit
			of microtubule-
			associated proteins 1A
			and 1B
708	7733	S77099	Drosophila	276	48
			pseudoobscura Jan A
709	7735	AF060862	Homo sapiens unknown	638	96
710	7741	AL133363	Arabidopsis thaliana	155	38
			putative protein
711	7743	AB034912	Homo sapiens WD-repeat	2483	100
			like sequence
712	7748	AF177145	Homo sapiens mammalian	2232	99
			inositol
			hexakisphosphate
			kinase 2
713	7749	X69910	Homo sapiens P63	2958	99
			protein
714	7750	U80736	Homo sapiens CAGF9	1657	100
715	7757	AC004997	Homo sapiens match to	2335	100
			ESTs AA667999
			(NID:g2626700),
			AA165465
			(NID:g1741481), Z45871
			(NID:g575105), and
			T84026 (NID:g712314);
			similar to various
			tre-like proteins
			including: AF040654
			(PID:g2746883), D13644
			(PID:g2104571),
			AL0211483
			(PID:g2815076) and
			Z797052 (PID:g2213552)
716	7759	AK000504	Homo sapiens unnamed	1045	100
			protein product
717	7760	AE003565	Drosophila	345	48
			melanogaster CG12756
			gene product
718	7760	AE003565	Drosophila	345	48
			melanogaster CG12756
			gene product
719	7764	AF193795	Homo sapiens vacuolar	960	100
			sorting protein
			VPS29/PEP11
720	7765	AJ222968	Mus musculus L-	120	30
			periaxin
721	7766	AK001456	Homo sapiens unnamed	4311	100
			protein product
722	7767	AE003431	Drosophila	322	36
			melanogaster CG15912
			gene product
723	7769	AK000505	Homo sapiens unnamed	2190	100
			protein product
724	7770	AE003525	Drosophila	383	42
			melanogaster CG7725
			gene product
725	7774	U37251	Homo sapiens	196	44
			Description: KRAB zinc
			finger protein; this
			is a splicing variant
			that contains a stop
			codon and frame shift
			between the KRAB box
			and the zinc finger
			region; Method:
			conceptual translation
			supplied by author
726	7779	AF233321	Mus musculus zinc	1864	94
			transporter like 1
727	7781	AE003790	Drosophila	339	86
			melanogaster CG3450
			gene product
728	7782	X95826	Homo sapiens mono-ADP-	1390	98
			ribosyltransferase
729	7783	M12098	Rattus norvegicus	155	25
			myosin heavy chain
730	7787	AF140683	Mus musculus F-box	2397	98
			protein FWD2
731	7792	AF151023	Homo sapiens HSPC189	1104	100
732	7795	AL117639	Homo sapiens	1342	99
			hypothetical protein
733	7801	AB007829	Homo sapiens CSR1	528	54
734	7807	AJ243972	Homo sapiens 6-	1317	100
			phosphogluconolactonase
735	7808	AB035863	Homo sapiens ATP	2324	99
			specific succinyl CoA
			synthetase beta
			subunit precursor
736	7819	AB015339	Homo sapiens	575	66
			HRIHFB2255
737	7824	AF163825	Homo sapiens pre-B	634	100
			lymphocyte protein 3
738	7826	AF201949	Homo sapiens 60S	868	100
			ribosomal protein L30
			isolog
739	7829	AF060862	Homo sapiens unknown	236	85
740	7832	AJ011373	Homo sapiens	549	100
			hypothetical protein
741	7839	AL031778	Homo sapiens dJ34B21.3	421	100
			(PUTATIVE novel
			protein)
742	7844	AK000452	Homo sapiens unnamed	1473	100
			protein product
743	7847	AK001851	Homo sapiens unnamed	2711	99
			protein product
744	7848	AK000510	Homo sapiens unnamed	1536	100
			protein product
745	7853	U89649	Chlamydomonas	244	34
			reinhardtii Mr19,000
			outer arm dynein light
			chain
746	7854	AL050008	Homo sapiens	591	56
			hypothetical protein
747	7856	AJ009985	Homo sapiens annexin	1675	99
			31 (annexin XXXI)
748	7862	AL080097	Homo sapiens	1363	100
			hypothetical protein
749	7865	AF224263	Heterodontus francisci	742	84
			HoxD8
750	7874	X63417	Homo sapiens IRLB	1037	100
751	7877	AE003485	Drosophila	622	53
			melanogaster CG11757
			gene product
752	7880	AK001939	Homo sapiens unnamed	2532	99
			protein product
753	7882	AF263614	Homo sapiens acetyl-	3493	99
			CoA synthetase
754	7884	AF022977	Caenorhabditis elegans	177	36
			contains similarity to
			leucine-rich repeats
			(LRR)
755	7886	AC006153	Homo sapiens similar	662	98
			to Aquifex aeolicus
			GTP-binding protein;
			similar to AE000771
			(PID:g2984292)
756	7888	AE003734	Drosophila	416	47
			melanogaster CG3337
			gene product
757	7889	AF110764	Mus musculus RS21-C6	655	75
758	7901	AE003459	Drosophila	507	59
			melanogaster CG9848
			gene product
759	7910	AF177476	Rattus norvegicus CDK5	1995	86
			activator-binding
			protein
760	7911	AL049946	Homo sapiens	3091	99
			hypothetical protein
761	7921	AL121733	Homo sapiens	314	39
			hypothetical protein
762	7923	AE003772	Drosophila	299	46
			melanogaster CG15525
			gene product
763	7924	AE003834	Drosophila	710	42
			melanogaster
			BcDNA:GH08789 gene
			product
764	7925	U16307	Homo sapiens glioma	329	40
			pathogenesis-related
			protein
765	7928	AF161457	Homo sapiens HSPC339	571	100
766	7929	AL050137	Homo sapiens	2319	100
			hypothetical protein
767	7930	AF223466	Homo sapiens HT015	831	66
			protein
768	7934	AL132965	Arabidopsis thaliana	286	30
			putative WD-40 repeat-
			protein
769	7938	AB024937	Homo sapiens LUNX	1284	100
770	7942	Y14768	Homo sapiens V-ATPase	579	100
			G-subunit like protein
771	7945	AL110235	Homo sapiens	870	100
			hypothetical protein
772	7946	L13291	Homo sapiens ADP-	802	46
			ribosylarginine
			hydrolase
773	7948	AK000771	Homo sapiens unnamed	1067	99
			protein product
774	7951	AE003808	Drosophila	319	54
			melanogaster CG8441
			gene product
775	7952	X92814	Homo sapiens	830	99
			homologous to rat
			HREV107 (ACC. NO.
			X76453)
776	7953	AF151638	Homo sapiens	1142	100
			phosphatidylcholine
			transfer protein
777	7954	AF059531	Homo sapiens protein	2679	99
			arginine N-
			methyltransferase 3
778	7957	AF161392	Homo sapiens HSPC274	370	79
779	7958	AL050100	Homo sapiens	165	53
			hypothetical protein
780	7961	AL117444	Homo sapiens	1991	100
			hypothetical protein
781	7965	X83006	Homo sapiens	208	40
			neutrophil gelatinase
			associated lipocalin
782	7966	U34973	Mus musculus protein	1131	95
			tyrosine phosphatase-
			like
783	7979	M86510	Schistosoma mansoni	327	43
			glutathione peroxidase
784	7986	AE000850	Methanobacterium	407	55
			thermoautotrophicum
			transcriptional
			regulator
785	7986	AE000850	Methanobacterium	406	55
			thermoautotrophicum
			transcriptional
			regulator
786	7988	AF161455	Homo sapiens HSPC337	742	98
787	7991	Z48795	Caenorhabditis elegans	247	38
			similarity to a
			thioredoxin-like
			protein from Bacillus
			subtilis (Swiss Prot
			accession number
			P35160)˜cDNA EST
			EMBL:D69151 comes from
			this gene˜cDNA EST
			EMBL:D69212 comes from
			this gene˜cDNA EST
			EMBL:D76199 comes from
			this gene˜cDNA EST
			EMBL:D76335 comes from
			this gene˜cDNA EST
			EMBL:D65648 comes from
			this gene˜cDNA EST
			EMBL:D65690 comes from
			this gene˜cDNA EST
			EMBL:D73198 comes from
			this gene˜cDNA EST
			EMBL:D73307 comes from
			this gene˜cDNA EST
			yk257e10.3 comes from
			this gene˜cDNA EST
			yk257e10.5 comes from
			this gene˜cDNA EST
			yk228e3.3 comes from
			this gene˜cDNA EST
			yk228e3.5 comes from
			this gene˜cDNA EST
			yk199h7.5 comes from
			this gene
788	7992	AJ005866	Homo sapiens Sqv-7-	1321	99
			like protein
789	7992	AJ005866	Homo sapiens Sqv-7-	1118	99
			like protein
790	7992	AJ005866	Homo sapiens Sqv-7-	891	99
			like protein
791	7992	AJ005866	Homo sapiens Sqv-7-	1016	99
			like protein
792	8003	AB040964	Homo sapiens KIAA1531	337	31
			protein
793	8014	AL117587	Homo sapiens	902	100
			hypothetical protein
794	8015	AL031010	Homo sapiens	968	100
			dJ422F24.1 (PUTATIVE
			novel protein similar
			to C. elegans C02C2.5)
795	8016	U28016	Mus musculus parathion	1624	87
			hydrolase
			(phosphotriesterase)-
			related protein
796	8017	AK001704	Homo sapiens unnamed	2207	99
			protein product
797	8019	AF117587	Manduca sexta unknown	348	71
798	8020	AB018260	Homo sapiens KIAA0717	3331	99
			protein
799	8022	AE003446	Drosophila	772	51
			melanogaster CG12121
			gene product
800	8022	AE003446	Drosophila	1074	52
			melanogaster CG12121
			gene product
801	8028	AL137520	Homo sapiens	2032	99
			hypothetical protein
802	8030	AF182076	Homo sapiens glioma	2418	100
			tumor suppressor
			candidate region
			protein 2
803	8038	AE003552	Drosophila	388	43
			melanogaster CG3967
			gene product
804	8042	AL159143	Homo sapiens	1045	60
			hypothetical protein
805	8045	L40357	Homo sapiens thyroid	509	100
			receptor interactor
806	8045	L40357	Homo sapiens thyroid	404	85
			receptor interactor
807	8046	Y18503	Homo sapiens XAP-5-	1672	100
			like protein
808	8047	AB041600	Mus musculus unnamed	1053	87
			protein product
809	8051	AL049688	Homo sapiens	2514	9B
			hypothetical protein
810	8059	AK001355	Homo sapiens unnamed	625	41
			protein product
811	8064	Z14122	Xenopus laevis XLCL2	455	77
812	8069	X67712	Psychrobacter	272	28
			immobilis
			triacylglycerol lipase
813	8074	AB033105	Homo sapiens KIAA1279	3221	99
			protein
814	8077	AK001963	Homo sapiens unnamed	952	100
			protein product
815	8078	AJ000217	Homo sapiens CLIC2	1286	99
816	8079	AB030505	Mus musculus UBE-1c2	1069	79
817	8084	AL080118	Homo sapiens	738	96
			hypothetical protein
818	8088	AE003829	Drosophila	641	71
			melanogaster CG11777
			gene product
819	8090	AL023553	Homo sapiens	557	100
			dJ347H13.4 (novel
			protein)
820	8091	AL109978	Homo sapiens	1679	100
			hypothetical protein
821	8099	AE003839	Drosophila	1037	58
			melanogaster CG8722
			gene product
822	8099	AE003839	Drosophila	678	53
			melanogaster CG8722
			gene product
823	8100	AF180681	Homo sapiens guanine	3597	100
			nucleotide exchange
			factor
824	8102	AK001433	Homo sapiens unnamed	944	100
			protein product
825	8103	M62419	Mus musculus clathrin-	2189	99
			associated protein
826	8103	AJ006219	Drosophila	1254	79
			melanogaster clathrin-
			associated protein
827	8104	AB006191	Mus musculus	362	78
			cornichon-like protein
828	8108	L03303	Oryctolagus cuniculus	1034	96
			small GTP-binding
			protein
829	8110	AB037823	Homo sapiens KIAA1402	4037	100
			protein
830	8116	A84493	unidentified unnamed	3309	100
			protein product
831	8117	AB030184	Mus musculus contains	1586	92
			transmembrane (TM)
			region and ATP binding
			region
832	8123	AL023694	Homo sapiens	663	100
			dJ511E16.2 (putative
			protein based on ESTs)
833	8130	AK001138	Homo sapiens unnamed	2182	99
			protein product
834	8130	AK001138	Homo sapiens unnamed	1858	99
			protein product
835	8143	AL022157	Homo sapiens SPIN	1233	100
			(SPINDLIN HOMOLOG
			(PROTEIN DXF34))
836	8143	AL022157	Homo sapiens SPIN	1233	100
			(SPINDLIN HOMOLOG
			(PROTEIN DXF34))
837	8154	AK001914	Homo sapiens unnamed	2176	99
			protein product
838	8155	AL020996	Homo sapiens	1492	100
			dJ317E23.2 (novel
			protein with remote
			similarity to
			KIAA0009)
839	8162	Z69637	Caenorhabditis elegans	240	57
			predicted using
			Genefinder-Similarity
			to E. coli hypothetical
			protein YCAC
			(SW:YCAC_ECOLI)˜cDNA
			EST yk555d12.3 comes
			from this gene
840	8163	AB023167	Homo sapiens KIAA0950	1664	100
			protein
841	8172	AE003527	Drosophila	737	40
			melanogaster CG4729
			gene product
842	8173	AK001350	Homo sapiens unnamed	1730	99
			protein product
843	8179	AF131852	Homo sapiens Unknown	473	100
844	8182	AF186593	Homo sapiens	406	27
			butyrophilin-like
845	8183	AC008015	Homo sapiens unknown	815	96
846	8184	AE003499	Drosophila	558	42
			melanogaster CG7860
			gene product
847	8185	AK001441	Homo sapiens unnamed	378	46
			protein product
848	8187	AJ272267	Homo sapiens choline	2449	100
			dehydrogenase
849	8188	AB001773	Ciona savignyi PEM-6	196	34
850	8190	AC004955	Homo sapiens supported	1618	85
			by ESTs T61992
			(NID:g665235) and
			W26450 (NID:g1307167)
			and Genscan
851	8190	AC004955	Homo sapiens supported	1618	85
			by ESTs T61992
			(NID:g665235) and
			W26450 (NID:g1307167)
			and Genscan
852	8192	AF113534	Homo sapiens HP1-BP74	2723	96
			protein
853	8193	AF232226	Danio rerio Dedd1	191	42
854	8197	AF132732	Homo sapiens unknown	1116	70
855	8197	AF132732	Homo sapiens unknown	1010	74
856	8199	AB040905	Homo sapiens KIAA1472	3062	99
			protein
857	8202	AB018268	Homo sapiens KIAA0725	3013	100
			protein
858	8203	AE003800	Drosophila	648	53
			melanogaster CG5742
			gene product
859	8208	AL117442	Homo sapiens	1344	100
			hypothetical protein
860	8209	AF040964	Homo sapiens unknown	3033	100
			protein IT1
861	8211	AB020713	Homo sapiens KIAA0906	4668	99
			protein
862	8214	AJ245417	Homo sapiens G5b	794	100
			protein
863	8217	AB037859	Homo sapiens KIAA1438	4761	99
			protein
864	8223	AE003469	Drosophila	352	45
			melanogaster CG13886
			gene product
865	8224	X58769	Homo sapiens V alpha	284	83
			gene segment
866	8226	AC012680	Arabidopsis thaliana	209	38
			putative protein
			phosphatase 2C
867	8227	AF132174	Drosophila	563	54
			melanogaster unknown
868	8229	AK000576	Homo sapiens unnamed	1342	100
			protein product
869	8232	AE003638	Drosophila	1420	47
			melanogaster CG5142
			gene product
870	8236	Y11710	Homo sapiens collagen	1048	97
			type XIV
871	8239	X82240	Homo sapiens T cell	617	100
			leukemia/lymphoma 1
872	8244	U42841	Caenorhabditis elegans	161	34
			short region of weak
			similarity to collagen
873	8245	AF023130	Homo sapiens Ras-GRF2	6413	100
874	8248	AJ131613	Homo sapiens	1470	99
			dicarboxylate carrier
			protein
875	8251	L27645	Danio rerio growth-	130	37
			associated protein
876	8253	AF141377	Mus musculus Ly-	527	81
			6/neurotoxin homolog
877	8260	AF217544	Xenopus laevis	1451	59
			ornithine
			decarboxylase-2
878	8262	AF136631	Homo sapiens neuritin	182	33
879	8268	X67098	Homo sapiens ORF1	493	100
880	8270	AB033064	Homo sapiens KIAA1238	1480	100
			protein
881	8272	AF154831	Rattus norvegicus PV-1	1403	60
882	8274	AF026528	Rattus norvegicus	915	99
			stathmin-like-protein
			RB3
883	8274	AF026530	Rattus norvegicus	1093	97
			stathmin-like-protein
			splice variant RB3″
884	8275	U35244	Rattus norvegicus	2981	96
			vacuolar protein
			sorting homolog r-
			vps33a
885	8277	AL353814	Arabidopsis thaliana	425	30
			putative protein
886	8281	AF157318	Homo sapiens AD-017	912	47
			protein
887	8283	AK000461	Homo sapiens unnamed	1594	100
			protein product
888	8289	AE003681	Drosophila	518	38
			melanogaster CG11986
			gene product
889	829S	AL031775	Homo sapiens dJ30M3.3	1902	100
			(novel protein similar
			to C. elegans
			Y63D3A.4)
890	8300	M21103	Ovis aries BIIIB4	484	82
			high-sulfur keratin
891	8303	Z85986	Homo sapiens	1143	75
			dJ108K11.3 (similar to
			yeast suppressor
			protein SRP40)
892	8304	U18762	Rattus norvegicus	890	52
			retinol dehydrogenase
			type I
893	8305	AF072467	Homo sapiens unknown	2495	100
894	8309	AB037779	Homo sapiens KIAA1358	2271	100
			protein
895	8318	AE003491	Drosophila	527	59
			melanogaster CG2453
			gene product
896	8319	AF136631	Homo sapiens neuritin	742	100
897	8321	AF207989	Homo sapiens orphan G-	2326	100
			protein coupled
			receptor
898	8322	Z97630	Homo sapiens dJ466N1.4	181	44
			(novel protein similar
			to ANK3 (ankyrin 3,
			node of Ranvier
			(ankyrin G)))
899	8323	U21549	Mus musculus	1280	68
			Ac39/physophilin
900	8325	AF036694	Caenorhabditis elegans	189	25
			CD4.4 gene product
901	8331	AF117814	Mus musculus odd-	945	68
			skipped related 1
			protein
902	8332	AE003442	Drosophila	360	50
			melanogaster CG2256
			gene product
903	8333	AK002084	Homo sapiens unnamed	2469	100
			protein product
904	8335	AL008729	Homo sapiens predicted	737	100
			protein dJ257A7.2
905	8336	AB032986	Homo sapiens KIAA1160	1458	100
			protein
906	8337	AK000523	Homo sapiens unnamed	1563	99
			protein product
907	8340	AE003658	Drosophila	436	47
			melanogaster CG7200
			gene product
908	8343	AK001344	Homo sapiens unnamed	1436	99
			protein product
909	8347	AK002182	Homo sapiens unnamed	1810	99
			protein product
910	8349	AK001715	Homo sapiens unnamed	715	99
			protein product
911	8351	AF155100	Homo sapiens zinc	2261	100
			finger protein NY-REN-
			21 antigen
912	8353	J05071	Bos taurus GTP-binding	356	100
			regulatory protein
			gamma-6 subunit
913	8355	AK001046	Homo sapiens unnamed	1173	99
			protein product
914	8361	AL050170	Homo sapiens	714	100
			hypothetical protein
915	8365	X64002	Homo sapiens RAP74	2661	99
916	8367	X04085	Homo sapiens catalase	2846	100
917	8369	AJ278124	Homo sapiens	1570	100
			hypothetical protein
918	8370	Z48745	Mus musculus ABC8	1101	69
919	8375	AF045564	Rattus norvegicus	1715	93
			development-related
			protein
920	8387	X97571	Mus musculus HCMV-	479	96
			interacting protein
921	8391	L08239	Homo sapiens located	2274	100
			at OATL1
922	8393	AF121863	Homo sapiens sorting	1964	100
			nexin 14
923	8393	AF121863	Homo sapiens sorting	1203	84
			nexin 14
924	8394	AL050101	Homo sapiens	2848	100
			hypothetical protein
925	8395	AE003681	Drosophila	1517	59
			melanogaster CG11990
			gene product
926	8396	Y18101	Mus musculus	1559	87
			macrophage actin-
			associated-tyrosine-
			phosphorylated protein
927	8398	AL050318	Homo sapiens dJ977B1.4	1224	100
			(novel protein similar
			to TGIF (TG-
			interacting factor
			(TALE family
			homeobox)))
928	8402	AB026264	Homo sapiens IMPACT	1694	100
929	8402	AB026264	Homo sapiens IMPACT	1123	100
930	8405	Z82062	Caenorhabditis elegans	431	42
			cDNA EST yk415c12.5
			comes from this
			gene˜cDNA EST
			yk526h3.3 comes from
			this gene˜cDNA EST
			yk599b1.3 comes from
			this gene
931	8406	AK001692	Homo sapiens unnamed	2492	99
			protein product
932	8409	AL035602	Arabidopsis thaliana	499	28
			putative protein
933	8410	AL050107	Homo sapiens	1342	100
			hypothetical protein
934	8414	AK000508	Homo sapiens unnamed	503	100
			protein product
935	8415	AL021453	Homo sapiens	856	100
			dJ821D11.3 (PUTATIVE
			protein)
936	8419	AJ276003	Homo sapiens GAR1	1216	100
			protein
937	8426	D26185	Bacillus subtilis	365	33
			unknown
938	8430	AC004874	Homo sapiens similar	957	100
			to N-
			acetylgalactosaminyl
			transferase; similar to
			Q07537 (PID:g1171989)
939	8431	AF199597	Homo sapiens A-type	1139	100
			potassium channel
			modulatory protein 1
940	8432	Y13148	Rattus norvegicus	1350	88
			PAG608
941	8433	M24852	Rattus norvegicus	124	46
			neuron-specific
			protein PEP-19
942	8434	AF146738	Rattus norvegicus	771	83
			testis specific
			protein
943	8438	AK000427	Homo sapiens unnamed	358	36
			protein product
944	8439	AB017644	Homo sapiens	919	85
			ubiquitin-conjugating
			enzyme E2
945	8441	AC006538	Homo sapiens BC41195_1	831	78
946	8450	AB004316	Bos taurus	1556	88
			mitochondrial
			methionyl-tRNA
			transformylase
947	8451	Z35094	Homo sapiens SURF-2	1354	97
948	8452	AL050275	Homo sapiens	2351	99
			hypothetical protein
949	8460	AC006014	Homo sapiens similar	1299	100
			to RFP transforming
			protein; similar to
			P14373 (PID:g132517)
950	8461	AC005099	Homo sapiens match to	469	100
			AI222572
			(NID:g3804775)
951	8462	V00507	Homo sapiens coding	984	100
			sequence of DHFR (1 is
			1st base in codon)
			(561 is 3rd base in
			codon)
952	8464	AL049709	Homo sapiens dJ18C9.2	3370	99
			(novel gene (locus
			D20S101) similar to
			Gamma-
			glutamyltranspeptidase,
			contains CCA
			trinucleotide repeat,
			based on Genscan and
			Fgenesh predictions.)
953	8465	AF173871	Mus musculus neuronal	977	94
			PAS3
954	8467	AF178983	Homo sapiens Ras-	433	97
			associated protein
			Rapl
955	8470	AB037858	Homo sapiens KIAA1437	1724	58
			protein
956	8471	AF109674	Rattus norvegicus late	846	74
			gestation lung protein
			1
957	8473	AF061346	Mus musculus Edp1	1077	64
			protein
958	8474	AK000343	Homo sapiens unnamed	1272	100
			protein product
959	8475	AF233582	Mus musculus GTPase	942	95
			Rab37
960	8476	AF195951	Homo sapiens signal	3127	98
			recognition particle
			68
961	8480	AL080168	Homo sapiens	2128	100
			hypothetical protein
962	8482	AE003713	Drosophila	207	44
			melanogaster CG14898
			gene product
963	8482	AE003713	Drosophila	91	60
			melanogaster CG14898
			gene product
964	8486	Z81592	Caenorhabditis elegans	426	55
			predicted using
			Genefinder
965	8488	AK000559	Homo sapiens unnamed	1319	99
			protein product
966	8492	Z71181	Caenorhabditis elegans	601	38
			similar to hydrolase
967	8494	Z81105	Caenorhabditis elegans	460	40
			similar to alpha/beta
			hydrolase fold˜cDNA
			EST EMBL:T02320 comes
			from this gene
968	8496	S94421	Homo sapiens T cell	478	100
			receptor eta-exon
969	8497	AL050214	Homo sapiens	949	99
			hypothetical protein
970	8499	AF161380	Homo sapiens HSPC262	772	100
971	8513	AE003802	Drosophila	423	44
			melanogaster CG14480
			gene product
972	8522	AK001972	Homo sapiens unnamed	520	38
			protein product
973	8526	U41012	Caenorhabditis elegans	172	24
			C06A6.3 gene product
974	8531	AE003635	Drosophila	1064	50
			melanogaster CG5336
			gene product
975	8533	AJ001019	Homo sapiens ring	1301	100
			finger protein
976	8542	AF003388	Caenorhabditis elegans	346	37
			R10F2.5 gene product
977	8544	AF178632	Homo sapiens FEM-1-	3261	100
			like death receptor
			binding protein
978	8565	AC006033	Homo sapiens similar	1195	100
			to MLN 64; similar to
			I38027 (PID:g2135214)
979	8565	AC006033	Homo sapiens similar	668	93
			to MLN 64; similar to
			I38027 (PID:g2135214)
980	8572	AB023811	Homo sapiens TU3A	351	55
981	8576	AE003802	Drosophila	362	37
			melanogaster CG4996
			gene product
982	8578	AF065441	Mus musculus FGF	174	24
			binding protein 1
983	8584	AK000367	Homo sapiens unnamed	3440	98
			protein product
984	8598	D87463	Homo sapiens KIAA0273	1396	76
985	8602	AL117600	Homo sapiens	2786	99
			hypothetical protein
986	8604	AJ249735	Homo sapiens claudin-6	1142	100
987	8609	X57560	Escherichia coli pspE	535	100
			protein
988	8612	AF169284	Homo sapiens LIM and	1997	100
			cysteine-rich domains
			protein 1
989	8637	AE003559	Drosophila	592	46
			melanogaster CG8576
			gene product
990	8640	AB024523	Homo sapiens basic	1206	100
			kruppel like factor
991	8643	X55989	Homo sapiens	737	99
			eosinophil cationic-
			related protein
992	8645	AF007151	Homo sapiens unknown	1481	100
993	8650	X52904	Escherichia coli open	359	100
			reading frame (AA 1-
			65)
994	8651	U19577	Escherichia coli	242	93
			galactonate
			dehydratase
995	8654	AL117660	Homo sapiens	447	100
			hypothetical protein
996	8655	AK001355	Homo sapiens unnamed	1553	100
			protein product
997	8657	AE003693	Drosophila	686	54
			melanogaster CG18347
			gene product
998	8665	AF044774	Homo sapiens	2681	99
			breakpoint cluster
			region protein 2
999	8668	AL008729	Homo sapiens predicted	416	100
			protein dJ257A7.1
1000	8671	X82693	Homo sapiens E48	620	96
			antigen
1001	8672	AE003499	Drosophila	692	51
			melanogaster CG7872
			gene product
1002	8692	AF131218	Homo sapiens	1493	100
			chromosome 16 open
			reading frame 5
1003	8706	AL021396	Homo sapiens	1375	100
			dJ971N18.2
1004	8716	AF196972	Homo sapiens JM24	2239	100
			protein
1005	8719	AF053356	Homo sapiens insulin	228	97
			receptor substrate
			like protein
1006	8743	AL050214	Homo sapiens	949	99
			hypothetical protein
1007	8764	AF153127	Gallus gallus SAPK	2442	89
			interacting protein
1008	8764	AF153127	Gallus gallus SAPK	1477	83
			interacting protein
1009	8764	AF153127	Gallus gallus SAPK	1651	86
			interacting protein
1010	8774	X56932	Homo sapiens 23 kD	1044	100
			highly basic protein
1011	8782	AF174605	Homo sapiens F-box	467	70
			protein Fbx25
1012	8796	AB033097	Homo sapiens KIAA1271	2824	100
			protein
1013	8827	Y17013	porcine endogenous	304	64
			retrovirus pol
1014	8842	AE003416	Unknown	1550	48
			Symbol = BG:DS01068.6;
			cDNA = method: ‘‘sim4’’,
			score: ‘‘1000.0’’,
			desc: ‘‘LD09509 LD
			Drosophila
1015	8842	AE003416	Unknown	1207	45
			symbol = BG:DS01068.6;
			cDNA = method: ‘‘sim4’’,
			score: ‘‘1000.0’’,
			desc: ‘‘LD09509 LD
			Drosophila
1016	8858	AL133215	Homo sapiens bA108L7.2	1322	99
			(novel protein similar
			to rat tricarboxylate
			carrier)
1017	8871	AK001721	Homo sapiens unnamed	1707	99
			protein product
1018	8921	U29495	Mus musculus Zfp61p	299	52
1019	8927	AK001344	Homo sapiens unnamed	1086	100
			protein product
1020	8942	AF146568	Homo sapiens MIL1	1936	100
			protein
1021	8994	AE003802	Drosophila	349	42
			melanogaster CG6410
			gene product
1022	9023	U10362	Homo sapiens GP36b	1001	55
			glycoprotein
1023	9028	AB018341	Homo sapiens KIAA0798	307	70
			protein
1024	9058	AE003442	Drosophila	636	54
			melanogaster CG10778
			gene product
1025	9058	AE003442	Drosophila	429	53
			melanogaster CG10778
			gene product
1026	9079	AB027004	Homo sapiens protein	1018	100
			phosphatase
1027	9079	AB027003	Mus musculus protein	378	84
			phosphatase
1028	9082	U64856	Caenorhabditis elegans	215	40
			weak similarity to TPR
			domains
1029	9084	AL110241	Homo sapiens	1240	97
			hypothetical protein
1030	9093	X76717	Homo sapiens MT-11	204	89
			protein
1031	9101	AK00181B	Homo sapiens unnamed	910	100
			protein product
1032	9103	AK001182	Homo sapiens unnamed	1752	94
			protein product
1033	9105	AF187016	Homo sapiens myosin	2303	99
			regulatory light chain
			interacting protein
			MIR
1034	9151	AB037730	Homo sapiens KIAA1309	894	35
			protein
1035	9161	AK001659	Homo sapiens unnamed	1886	99
			protein product
1036	9172	Plasmodium	3′ end., gene product	178	23
		falciparum
1037	9174	AK001324	Homo sapiens unnamed	2657	99
			protein product
1038	9204	AF161548	Homo sapiens HSPC063	1018	98
1039	9234	AB041581	Mus musculus unnamed	1758	95
			protein product
1040	9235	X98507	Homo sapiens myosin I	5288	99
			beta
1041	9239	AL133107	Homo sapiens	1388	100
			hypothetical protein
1042	9256	D90869	Escherichia coli	2047	100
			similar to
1043	9276	A12029	Homo sapiens MRP-14	613	100
1044	9345	AC005328	Homo sapiens R26660_1,	870	74
			partial CDS
1045	9379	AC024876	Caenorhabditis elegans	829	61
			contains similarity to
			SW:RPB1_CRIGR
1046	9435	AB014536	Homo sapiens KIAA0636	1876	64
			protein
1047	9437	U85055	Mus musculus rap1/rap2	2103	90
			interacting protein
1048	9469	AP000060	Aeropyrum pernix 264aa	108	33
			long hypothetical
			protein
1049	9500	AE003638	Drosophila	583	48
			melanogaster CG12404
			gene product
1050	9502	X78927	Homo sapiens zinc	3865	99
			finger protein
1051	9520	AL163279	Homo sapiens homolog	5035	99
			to cAMP response
			element binding and
			beta transducin family
			proteins
1052	9541	Z48475	Homo sapiens	3160	99
			glucokinase regulator
1053	9541	Z48475	Homo sapiens	2682	97
			glucokinase regulator
1054	9548	AF195764	Homo sapiens	2055	99
			megakaryocyte - enhanced
			gene transcript 1
			protein; MEGT1 protein
1055	9556	AC004382	Homo sapiens Unknown	1593	100
			gene product
1056	9556	AC004382	Homo sapiens Unknown	984	100
			gene product
1057	9575	AL117352	Homo sapiens	2581	99
			dJ876B10.3 (novel
			protein similar to C.
			elegans T19B10.6
			(Tr:Q22557))
1058	9589	AE003454	Drosophila	218	43
			melanogaster CG10440
			gene product
1059	9599	AJ245621	Homo sapiens CTL2	3728	99
			protein
1060	9602	AE003673	Drosophila	440	40
			melanogaster CG1939
			gene product
1061	9606	X05562	Homo sapiens alpha-2	5908	99
			chain precursor (AA -
			25 to 1018) (3416 is
			2nd base in codon)
1062	9622	Z98048	Homo sapiens	1296	99
			dJ408N23.4 (novel DnaJ
			domain protein)
1063	9623	AF154415	Homo sapiens FLASH	10253	100
1064	9646	U20286	Rattus norvegicus	1567	70
			lamina associated
			polypeptide 1C
1065	9747	AB033101	Homo sapiens KIAA1275	5625	99
			protein
1066	9773	AL117337	Homo sapiens	250	60
			bA393J16.1 (zinc
			finger protein 33a
			(KOX 31))
1067	9785	AC005328	Homo sapiens R26660_1,	1126	100
			partial CDS
1068	9801	AB033092	Homo sapiens KIAA1266	3067	99
			protein
1069	9811	AE003633	Drosophila	961	76
			melanogaster CG14939
			gene product
1070	9843	AL080080	Homo sapiens	1508	100
			hypothetical protein
1071	9854	AB037360	Homo sapiens ANKHZN	5734	95
1072	9854	AB037360	Homo sapiens ANKHZN	959	97
1073	9864	AF237676	Mus musculus G beta-	1721	96
			like protein GBL
1074	9864	AF237676	Mus musculus G beta-	1043	70
			like protein GBL
1075	9871	U26358	Rattus norvegicus	137	36
			S100A1 gene product
1076	9879	AF212162	Homo sapiens ninein	10369	99
1077	9881	AK000463	Homo sapiens unnamed	1252	99
			protein product
1078	98B5	AC004890	Homo sapiens similar	542	86
			to zinc finger
			proteins; similar to
			BAA24380
1079	9901	AF187989	Homo sapiens zinc	2665	99
			finger protein ZNF223
1080	9912	AC035150	Homo sapiens Zinc	3459	100
			finger protein ZNF221
1081	9916	Z82095	Caenorhabditis elegans	702	54
			similar to PDZ domain
			(Also known as DHR or
			GLGF). ˜cDNA EST
			EMBL:M75803 comes from
			this gene
1082	9921	AF117610	Mus musculus inner	583	58
			centromere protein
			INCENP
1083	9925	X90840	Homo sapiens axonal	4584	99
			transporter of
			synaptic vesicles
1084	9930	AF148848	Homo sapiens myoneurin	3208	99
1085	9949	AB033037	Homo sapiens KIAA1211	3939	98
			protein
1086	9951	AK001605	Homo sapiens unnamed	647	96
			protein product
1087	9959	AF140342	Homo sapiens	37	36
			autoantigen SS-N
1088	9973	AK001753	Homo sapiens unnamed	193	82
			protein product
1089	9982	AL133396	Homo sapiens	962	100
			dJ1068H6.4 (prion
			protein like protein
			doppel)
1090	9994	AK001192	Homo sapiens unnamed	2550	100
			protein product
1091	10021	AK001842	Homo sapiens unnamed	546	100
			protein product
1092	10041	Z54096	Schizosaccharomyces	320	40
			pombe hypothetical
			coiled-coil protein
1093	10045	AK001122	Homo sapiens unnamed	227	43
			protein product
1094	10067	Y12090	Lycopersicon	1040	42
			esculentum putative
			3,4-dihydroxy-2-
			butanone kinase
1095	10073	X81058	Mus musculus tex261	1010	99
1096	10112	AB012084	Mus musculus ITM	194	30
1097	10117	AB030251	Homo sapiens GTPase	3233	99
			activating protein ID-
			GAP
1098	10132	AJ01058S	Rattus rattus PTB-like	2684	99
			protein
1099	10169	X75760	Drosophila	364	30
			melanogaster LRR47
1100	10217	U76618	Mus musculus N-RAP	804	48
1101	10226	AC005578	Homo sapiens F20887_1,	835	65
			partial CDS
1102	10232	D90832	Escherichia coli	360	100
			ORF_ID:o341#12;
			similar to
1103	10237	X01563	Escherichia coli L5	911	100
			(rp1E)(aa 1-179)
1104	10279	AL133206	Homo sapiens	1820	99
			hypothetical protein

SEQUENCE LISTING
The patent contains a lengthy “Sequence Listing” section. A copy of the “Sequence Listing” is available in electronic form from the USPTO
web site (http://seqdata.uspto.gov/sequence.html?DocID=06569662B1). An electronic copy of the “Sequence Listing” will also be available from the
USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).

Claims

1. The isolated polypeptide of SEQ ID NO: 1105.

2. A composition comprising the polypeptide of claim 1 and a carrier.