Claims
- 1. An isolated polynucleotide sequence which encodes a polypeptide having the amino acid sequence of SEQ ID NO:2.
- 2. The polynucleotide sequence of claim 1, wherein the polynucleotide is DNA.
- 3. The polynucleotide sequence of claim 1, wherein the polynucleotide is RNA.
- 4. A recombinant expression vector containing the polynucleotide of claim 1.
- 5. The expression vector of claim 4, wherein the vector is a plasmid.
- 6. The vector of claim 4, wherein the polynucleotide sequence is from L. alstoni.
- 7. A host cell transformed with the expression vector of claim 4.
- 8. The host cell of claim 7, wherein the cell is a prokaryote.
- 9. The prokaryote of claim 8, which is E. coli.
- 10. The host cell of claim 7, wherein the cell is a eukaryote.
- 11. A method of producing OmpL2 polypeptide which comprises:
- a. transforming a host with the polynucleotide of claim 1;
- b. expressing the polynucleotide in the host; and
- c. recovering the OmpL2 polypeptide.
- 12. The method of claim 11, wherein the host is a prokaryote.
- 13. An isolated polynucleotide selected from the group consisting of:
- a. the nucleotide sequence of SEQ ID NO:1, wherein T can also be U; and
- b. nucleic acid sequences complementary to the nucleotide sequence of SEQ ID NO:1.
- 14. A kit useful for the detection of OmpL2 nucleic acid, the kit comprising carrier means being compartmentalized to receive in close confinement therein one or more containers comprising a first container containing a polynucleotide of claim 13 which hybridizes to Leptospira OmpL2 nucleic acid.
Government Interests
This invention was made with Government support by the Veteran's Administration Research Advisory Group and Grant Nos. Al-21352, Al-29733, and Al-12601 awarded by the National Institutes of Health. The Government has certain rights in the invention.