Claims
- 1. An isolated and purified nucleic acid molecule comprising a nucleotide sequence encoding a modified human ciliary neurotrophic factor (CNTF) with a modification consisting of Gln63→Arg.
- 2. An expression vector comprising the isolated nucleic acid molecule of claim 1.
- 3. An isolated host cell transformed with the expression vector of claim 2.
- 4. A method for producing a modified human ciliary neurotrophic factor comprising: (a) growing an isolated host cell transformed with an expression vector comprising the isolated nucleic acid molecule of claim 1 under conditions such that the nucleic acid molecule is expressed by the host cell and produces the modified human ciliary neurotrophic factor and (b) isolating the expressed, modified human ciliary neurotrophic factor.
- 5. The method according to claim 4, wherein said host cell is a eukaryotic cell.
- 6. The method according to claim 4, wherein said host cell is a prokaryotic cell.
Parent Case Info
This application is a continuation of U.S. Ser. No. 08/308,736 filed Sep. 19, 1994 now U.S. Pat. No. 5,846,935, issued Dec. 8, 1998, which is a continuation-in-part of U.S. Ser. No. 07/959,284 filed Oct. 9, 1992, now U.S. Pat. No. 5,349,056 issued Sep. 20, 1994. The present invention relates to therapeutic CNTF-related polypeptides useful for the treatment of neurological or other diseases or disorders.
Non-Patent Literature Citations (2)
Entry |
Panayotatos et al J. Biol. Chem 268:19000-19003, 1993.* |
Rudinger, In “Peptide Hormones” (Ed. J.A. Parsons) University Park Press, Baltimore, pp. 1-7, 1976. |
Continuations (1)
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Number |
Date |
Country |
Parent |
08/308736 |
Sep 1994 |
US |
Child |
09/197349 |
|
US |
Continuation in Parts (1)
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Number |
Date |
Country |
Parent |
07/959284 |
Oct 1992 |
US |
Child |
08/308736 |
|
US |