Claims
- 1. An isolated DNA consisting essentially of nucleotides encoding a protein having the amino acid sequence of SEQ ID NO:2.
- 2. An isolated DNA consisting of nucleotides encoding a protein having the amino acid sequence of SEQ ID NO:2.
- 3. An isolated DNA comprising the nucleotide sequence of SEQ ID NO:1 nucleotides 151 to 612 or its complement.
- 4. An isolated DNA comprising the nucleotide sequence of SEQ ID NO:1 or its complement.
- 5. A vector containing a DNA of any one of the claims 1 to 4.
- 6. A vector for expressing the leucine responsive protein (lrp) of Corynebacterium glutamicum comprising a promotor and a coding sequences, wherein said coding sequence consists of the DNA of any one of the claims 1 to 4.
- 7. A bacterial host cell transformed with the vector of claim 9 or 10.
- 8. An isolated polypeptide consisting essentially of the amino acid sequence of SEQ ID NO:2.
- 9. A process for the production of an L-amino acid, comprising:
a) fermenting bacteria producing said amino acid, wherein the lrp gene in said bacteria is attenuated or amplified; b) allowing said L-amino acid to accumulate in the fermentation medium or in said bacteria; c) isolating said L-amino acid.
- 10. The process of claim 9, wherein said bacteria contain at least one additional gene of the biosynthetic pathway of said L-amino acid that has been amplified.
- 11. The process of claim 9, wherein a metabolic pathway that reduces the formation of said L-amino acid is suppressed in said bacteria.
- 12. The process of claim 9, wherein said bacteria have been transformed with a plasmid vector comprising a sequence that codes for lrp.
- 13. The process of claim 9, wherein a deletion or insertion is made in said lrp gene and wherein said deletion or insertion attenuates the gene.
- 14. The process of claim 10, wherein said bacteria are coryneform bacteria.
- 15. The process of claim 11, wherein said bacteria are coryneform bacteria.
- 16. The process of claim 12, wherein said bacteria are coryneform bacteria.
- 17. The process of claim 13, wherein said bacteria are coryneform bacteria.
Priority Claims (1)
Number |
Date |
Country |
Kind |
199 47 792.2 |
Oct 1999 |
DE |
|
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] The present application is a continuation-in-part of U.S. Ser. No. 09/456,304, filed on Dec. 8, 1999 and claims priority to German application DE 199 47 792.2, filed on Oct. 5, 1999.
Continuation in Parts (1)
|
Number |
Date |
Country |
Parent |
09456304 |
Dec 1999 |
US |
Child |
10134640 |
Apr 2002 |
US |