NYSGXRC:A Large Scale Center for the PSI

Information

  • Research Project
  • 7640674
  • ApplicationId
    7640674
  • Core Project Number
    U54GM074945
  • Full Project Number
    5U54GM074945-06
  • Serial Number
    74945
  • FOA Number
    RFA-GM-05-001
  • Sub Project Id
  • Project Start Date
    7/1/2005 - 18 years ago
  • Project End Date
    6/30/2011 - 12 years ago
  • Program Officer Name
    PREUSCH, PETER
  • Budget Start Date
    7/1/2009 - 14 years ago
  • Budget End Date
    6/30/2011 - 12 years ago
  • Fiscal Year
    2009
  • Support Year
    6
  • Suffix
  • Award Notice Date
    7/1/2009 - 14 years ago
Organizations

NYSGXRC:A Large Scale Center for the PSI

DESCRIPTION (provided by applicant): Structural GenomiX, Inc., Albert Einstein College of Medicine, the Department of Biology at Brookhaven National Laboratory, Columbia University, Sloan Kettering Institute, and the University of California at San Francisco comprise the New York Structural Genomics Research Consortium (NYSGXRC). This U54 application proposes to establish and operate a Large Scale Center for Phase 2 of the Protein Structure Initiative (PSI-2), as specified in RFA-GM-05-001. Together, members of the NYSGXRC will establish and operate a fully-integrated, high-throughput center for the following activities: (i) protein family classification and target selection, (ii) protein expression and solubility testing, (iii) E. coli and baculovirus fermentation, (iv) purification and biophysical characterization, (v) crystallization, (vi) synchrotron X-ray diffraction data collection, (vii) X-ray phase determination via molecular replacement, multi- and single-wavelength anomalous dispersion measurements, and isomorphous replacement, (viii) model building and refinement, (ix) PDB deposition of atomic coordinates and experimental structure factors, (x) comparative protein structure modeling, and (xi) functional annotation and dissemination of results. Targets for this effort will be derive from three sources, including the PSI-2 Centralized Target Selection Committee (ca. 70% of capacity), an NYSGXRC biomedical research program focused on the phosphatase superfamily (ca. 15% of capacity), and targets solicited from the larger scientific community (ca. 15% of capacity). A program of technology development will also be undertaken to both increase efficiency/throughput and accelerate structure determination for challenging, multi-domain eukaryotic proteins. At peak production, the NYSGXRC will be capable of determining 200 or more structures annually at an average cost approaching $50,000-60,000/structrure for proteins that can be expressed in E. coli. The long-term goal of the NYSGRC is to contribute substantially to the 5,000-10,000 structure objective of PSI-2.

IC Name
NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES
  • Activity
    U54
  • Administering IC
    GM
  • Application Type
    5
  • Direct Cost Amount
  • Indirect Cost Amount
  • Total Cost
    9596801
  • Sub Project Total Cost
  • ARRA Funded
    False
  • CFDA Code
    859
  • Ed Inst. Type
  • Funding ICs
    NIGMS:9596801\
  • Funding Mechanism
    Research Centers
  • Study Section
    ZGM1
  • Study Section Name
    Special Emphasis Panel
  • Organization Name
    ELI LILLY AND COMPANY
  • Organization Department
  • Organization DUNS
    006421325
  • Organization City
    INDIANAPOLIS
  • Organization State
    IN
  • Organization Country
    UNITED STATES
  • Organization Zip Code
    46285
  • Organization District
    UNITED STATES