Patent Application
20180321160
The invention relates to a sensor device for measuring at least one first analyte and a second analyte.
The German published Patent Application DE 10 2014 112 972 A1 discloses a sensor and a membrane therefor. The membrane includes a sensor element having at least one functional layer with a sensor-specific substance. The sensor-specific substance can be such that contact with an analyte to be measured changes the chemical or physical properties of the sensor-specific substance. The sensor-specific substance may for example be a phosphorescent dye or an indicator dye. The sensor element may be a multilayer structure having at least two sensor components, examples of which are a polymer layer with encapsulated pigments, a light-blocking layer, and a pH-buffer layer.
The German translation DE 601 28 279 T2 of European Patent EP 1 130 382 B1 discloses an arrangement of sensors for measuring plural analytes, wherein a processor is provided for signal processing, in order to determine the concentration of the respective analytes despite adverse interaction between the sensors.
The German published Patent Application DE 10 2014 103 721 A1 discloses an optical sensor for measuring concentrations. The sensor has a sensitive element in a sensor head, and electronic components, separated from the sensor head. An optical fibre is connecting the sensor head and the electronic components. The optical sensor furthermore comprises a thermal sensor in close vicinity of the sensitive element.
The U.S. Pat. No. 4,270,091 discloses an apparatus for monitoring plural gases simultaneously by the use of plural filters of different transmission and a separate detector for each filter. Alternatively, plural gases can be monitored sequentially by plural filters selectively insertable between the excitation source and a single detector.
US Patent Application Publication No. 2013/0109040, discusses an in vitro sensor for detection of at least one analyte. The sensor comprises an analyte-detection optode membrane, an analyte-permeable membrane, and a plurality of microbeads. The analyte-permeable membrane covers the optode membrane, which is arranged in a cavity formed in a substrate. The sensor can comprise plural such optode membranes in respective cavities. The microbeads, arranged for example in the optode membrane or the analyte permeable membrane, facilitate diffuse reflectance, for example by filtering out the colour of an underlying biological fluid sample.
U.S. Pat. No. 6,256,522, based on U.S. patent application Ser. No. 08/516,257, refers to a sensor capsule containing at least one dye used to measure a corresponding analyte. The wall of the capsule is formed by a membrane permeable to the analyte. The capsule is intended to be implanted under the skin of a patient. As an alternative to a capsule design, also a polymer-gel structure with corresponding reporter molecules on its outside is disclosed.
German Patent DE 10 2013 108 659 B3 discloses a sensor, for example embodied as a membrane, holding a medium containing a dye which exhibits an optical behaviour dependent on an analyte. The sensor also has a restriction means to mechanically restrict a change of volume of the medium. An osmolality in the medium is set to a value greater than a maximum osmolality of a sample for which the sensor is intended. The cooperation of the restriction means and the set osmolality in the medium result in a reduced cross-sensitivity of the sensor to osmolality.
The article “Simultaneous color sensing of O2 and pH using a smartphone” by Xu Wei et al., Sensors and Actuators B: Chemical, International Journal Devoted to Research and Development of Physical and Chemical Transducers, vol. 220, 4 Jun. 2015, pages 326-330, discloses a method for measuring both O2 and pH by recording and analysing, with a smartphone, a colour image of a sensor film, in which a dye sensitive to oxygen is immobilised and to which a pH-sensitive dye has been covalently bonded.
Many different methods of conducting measurements for determining, using optical sensors, the concentration or the partial pressure of a substance, such a substance then being also referred to as an analyte, are well known. Examples, which in no way should be construed to be limiting, can be found in the International Patent Application PCT/EP2012/070552, published as WO 2013/068215 A1, or in International Patent Application PCT/IB2014/063765, published as WO 2015/025243 A1, or European Patent EP 1 000 345 B1 based on European Patent Application EP 98945125.7, as well as in references cited therein. Such methods can also be employed with the sensor device according to the invention.
Correspondingly known are many kinds of optical sensors, which differ in their chemical composition, the optical behaviour they show, and the way the targeted analyte affects this optical behaviour. Examples can also be found in the references just cited.
The sensors this application relates to include a substance, also referred to as a dye, which shows the optical behaviour used in measuring an analyte, which means determining a concentration of the analyte, for example, if the analyte is a substance in a solution, or determining a partial pressure of the analyte, usually if the analyte is a gas in a mixture of gases or in a liquid medium. Determining a concentration, or partial pressure, of the analyte includes determining, within defined error bounds, a value for the concentration, or partial pressure, respectively, but to also includes only determining that a value for concentration, or partial pressure, is within a given range; this range may have an upper bound and a lower bound, or only just an upper bound or just a lower bound.
The optical behaviour of the dye, depending on an analyte and thus used for measuring this analyte, can for example include reflectivity, absorption or colour of the dye, and can as further example include luminescence, luminescence comprising at least fluorescence and phosphorescence. This means that in these respective cases, in dependence on the concentration or partial pressure of the analyte, the coefficients of reflection or absorption of the dye for light of a given wavelength change, or that the colour of the dye changes, which can be quantitatively determined by known colorimetric methods, or that the luminescence behaviour of the dye changes. The latter means that a luminescence effect the dye shows in reaction to excitation, for example excitation by light in a particular wavelength region, changes. The change can for example be a change of the intensity and/or wavelength of the luminescence light, or a change of a decay time of the luminescence. The decay can for example be a decay of intensity or the degree of polarization of the luminescence.
These sensors, when used in specific applications or measurement settings, can come in, and are commercialized in, many different shapes, for example as sensor spots or sensor patches. A further mode of use is as part of a probe inserted via dedicated ports into vessels, for example bio-reactors. The use of dedicated ports is in particular advisable if the measurements, or the processes to be monitored by the measurements, have to be carried out in a sterile and/or decontaminated environment. While a vast number of different bio-reactors is available on the market, these bio-reactors usually exhibit only a small number of ports, if compared with the number of quantities of interest in the processes taking place within the bio-reactors, and the corresponding number of sensors that have to be placed into the bio-reactor. The number of quantities of interest has strongly increased over the past 15 years or so, a tendency which still prevails. While bio-reactors are a prominent example, the need for monitoring a large number of quantities, in particular concentrations or partial pressures of various substances, can also arise in different settings, implying that the present invention is not limited to application in bio-reactors.
It therefore is an object of the invention to provide a sensor device capable of measuring plural analytes, while, if used in vessels via dedicated ports, only requiring a single port. Moreover, effects of sensor poisoning should be avoided.
This object is achieved by a sensor device for measuring at least one first analyte and a second analyte. The sensor device has a cavity in which a buffer is contained. A membrane limits the cavity at least on one side of the cavity. The membrane includes at least one first dye. Each of the at least one first dye exhibits an optical behaviour which depends on a respective first analyte. Furthermore, a second dye is mixed with the buffer. The second dye exhibits an optical behaviour which depends on the pH-value in the buffer, wherein the pH-value in the buffer in turn depends on a second analyte. A reservoir of the buffer and the second dye is used, wherein the reservoir is in diffusive contact with the cavity. Preferably, the buffer is a pH-buffer solution. Preferably, the reservoir is of annular shape. Preferably, the reservoir is at least partially surrounded by an opaque layer.
As a result of the dependence of the optical behaviour of each of the at least one first dye on a respective first analyte, each of the at least one first dye can be employed in measuring the respective first analyte.
Via the dependence mentioned above for the second analyte, i.e. the second analyte affecting the pH-value in the buffer, the pH-value in the buffer affecting the optical behaviour of the second dye, the second analyte can be measured.
Therefore, the sensor device according to the invention can measure not only at least one first analyte, but also a second analyte. This, in particular, also, if the optical behaviours of the dyes available for measuring the at least one first and the second analyte are affected via different transduction principles by the respective analytes.
An analyte affecting the pH-value of a buffer can be measured as the second analyte, by a pH-sensitive second dye mixed with the buffer, while further analytes, even if they do not suitably affect a pH-value of a buffer, can be measured via suitable dyes in the membrane. As the membrane is at the same time used to limit the cavity, at least on one side, thus preventing the buffer with the second dye from escaping from the cavity at this at least one side, the dyes can easily be combined into a single sensor device.
An example of a transduction principle not involving a pH-value is non-radiative energy transfer from a dye to an analyte. For instance, a luminescent dye, elevated into an excited state by absorbing energy in form of excitation light, may release energy as luminescence light. If, as a further channel for energy release, the dye can also transfer energy to the analyte in a non-radiative way, the luminescence behaviour of the dye changes in presence of the analyte. Often the decay time of the luminescence is used for measuring the analyte. If there is an additional channel for the dye to release energy, the luminescence will decay faster. In the case of non-radiative transfer of energy to the analyte, the luminescence will decay the faster the higher the number of analyte molecules that can interact with the dye, i.e. the higher the concentration or partial pressure of the analyte. This and further transduction principles are well known in the art.
A general requirement for the sensor device to function properly is that the at least one first analyte and the second analyte reach the respective dyes provided to change their optical behaviour in reaction to the respective analyte. When measuring, the membrane is brought into contact with a medium containing the analytes to be measured. The membrane must be of such configuration that the analytes can enter the membrane, that each of the at least one first analyte can reach the respective one of the at least one first dye within the membrane, and such that at least the second analyte can pass through the membrane and reach the second dye in the cavity. The analytes enter in and pass through the membrane usually by diffusion. When this paragraph speaks of an analyte reaching a dye, what is meant is that the analyte reaches a part of the sensor device where it can interact with the at least one first dye or the second dye in such a way that the optical behaviour of the respective dye can be affected by the corresponding analyte. This interaction between the dye and the respective analyte may be a direct interaction between dye and analyte, or an indirect interaction. An example of an indirect interaction is the above described case of the second dye, where the second analyte changes the pH-value of a buffer, and the second dye reacts to the change of the pH-value. An example of a direct interaction is the above described non-radiative energy transfer from the dye to the analyte. Although the analytes must be able to enter into the membrane, the membrane may in particular be hydrophobic and impermeable to ions, in order to protect the composition of the buffer-dye mixture in the cavity and also the at least one first dye in the membrane from unwanted exposure to the medium.
According to the invention, the sensor device includes a reservoir of the buffer and second dye, wherein the reservoir is in diffusive contact with the cavity. In sensors based on the cooperation of a buffer and a dye, as is the case for the second dye in the present invention, the chemical composition of the buffer, its osmolality, the pKa-values of buffer and dye, as well as their concentrations are chosen according to the desired target analyte and the desired range of sensitivity. A disadvantage of such sensors, for example in bio-technological processes, is the “poisoning” of the sensor. “Poisoning” of a sensor refers to substances other than the target analyte entering the sensor, in particular the buffer, and changing the chemical conditions there, so that the buffer and the dye, when used for a measurement, no longer operate at the intended conditions, for example the conditions at which calibration of the sensor was performed. This leads to measurement errors and therefore should be avoided. The effect accumulates over time, reducing the lifetime of the sensor. The reservoir of buffer and second dye is provided to counter the sensor poisoning. Substances other than the target analyte entering the cavity can be removed from the cavity into the reservoir by diffusion, while, also by diffusion, fresh buffer and second dye are supplied to the cavity from the reservoir.
In an example embodiment, the cavity is limited by a transparent element on a side of the cavity on which the cavity is not limited by the membrane. In this way the cavity is limited on one side by the membrane and on a further side by the transparent element. As used herein, “transparent” is intended to mean that at least light of the wavelengths used in measurements with the sensor device are transmitted through the transparent element at at least 50% intensity, typically with over 90% intensity. This includes at least the wavelengths of light emitted from a light source for excitation light; such a light source in embodiments may form part of the sensor device. It includes further the wavelengths associated with the optical behaviour of the at least one first and the second dye in the sensor device, for example the wavelengths of the luminescence light in case the optical behaviour is a luminescence phenomenon. The transparent element may of course exhibit a high transmission, above 50% or above 90%, for example, for a wider range of wavelengths. The transparent element may for example be made of a glass or a plastic. Apart from limiting the cavity on one side, the transparent element may also function as a window for excitation light to impinge on the at least one first dye and the second dye in the sensor element, and/or as window for light from the at least one first dye and the second dye in the sensor element, i.e. for light emitted by or having interacted with at least one of the at least one first dye or the second dye. The transparent element may furthermore be shaped so as to shape the light passing through it, for example, the transparent element may be shaped like, and also function as, a lens.
In another example embodiment, the reservoir is of annular shape. The reservoir may, in particular, be attached to the cavity in such a way that light propagating towards the dyes as well as in the reverse direction passes through an area surrounded by the annular reservoir. With an annular reservoir it is possible to counter sensor poisoning throughout the cavity in a more homogeneous fashion than would be possible with a reservoir in diffusive contact with the cavity only at one location. The reservoir may be at least partially surrounded by an opaque layer. An opaque layer protects the content of the reservoir, in particular the second dye, from light, especially from light used for excitation of the second dye in the cavity. As some dyes tend to be destroyed with increasing exposure to light, fully or partially surrounding the reservoir with an opaque layer contributes to an increased lifetime of the sensor.
An opaque layer fully or partially surrounding the reservoir may also be arranged so as to shield any detection system for light from the first dye and/or for light from the second dye against light from within the reservoir. Due to the dimensions of the reservoir, which normally is of larger volume than the cavity, the second analyte will typically take longer to diffuse through the reservoir than through the cavity. This implies that the buffer and second dye within the reservoir, considered as a whole, show a slower response to the second analyte, especially a slower response to changes in concentration or partial pressure of the second analyte, than the buffer and second dye in the cavity. Capturing also light from within the reservoir with the above mentioned detection system therefore would lead to a strong hysteresis of the sensor signal. Such a hysteresis is advantageously avoided by the opaque layer. In case the reservoir is protected against excitation light by the opaque layer, there will be no light from within the reservoir against which the detection system needs to be shielded; in this case a strong hysteresis of the sensor signal is avoided as well.
An annular reservoir at least partially surrounded by an opaque layer and attached to the cavity in such a way that light propagating towards the dyes as well as in the reverse direction passes through an area surrounded by the annular reservoir can in addition function as a collimator, contributing to a better controlled propagation of light within the sensor device.
In an embodiment the sensor device includes an optics section, arranged to receive light from the at least one first dye and the second dye. For each of the at least one first dye, the optics section includes a dichroic mirror and an associated photodetector. Each dichroic mirror is provided to direct light from the corresponding first dye to the associated photodetector. The optics section in this embodiment furthermore includes a dichroic mirror and an associated photodetector for the second dye; this dichroic mirror is provided to direct light from the second dye to the associated photodetector.
In another example embodiment, the optics section includes a beam splitter and an associated photodetector for generating a reference signal for a light source. The light source is provided for generating the excitation light used in measurements with the sensor device. The light source may be an external light source or a light source integrated into the sensor device. The beam splitter directs a part of the light generated by the light source to the associated photodetector, for monitoring the intensity of the light sent towards the dyes from the light source.
The beam splitter may in particular be configured to direct 0.5% to 6% of the light intensity impinging on it from the light source to the associated photodetector, while letting the remaining light pass on towards the dyes.
In another example embodiment, an optical waveguide is provided for guiding light from the optics section towards the at least one first dye and the second dye. Alternatively or additionally, the optical wave guide may be used for guiding light emitted from or having interacted with at least one of the at least one first dye to the optics section, e.g. luminescence light emitted from at least one first dye or light reflected or scattered from at least one first dye. In the same manner, the optical waveguide may guide light emitted from or having interacted with the second dye to the optics section. The reservoir of the sensor device may be arranged around the optical waveguide, or, put differently, the optical waveguide may pass through the reservoir.
In other example embodiments, in addition to an optics section as described above, the sensor device includes a control and evaluation section for controlling the optics section and processing signals received from the photodetectors of the optics section. Processing the signals from the photodetectors may in particular include converting the signals into any desired data format, in particular a digital data format, for communicating the signals received from the photodetectors, which in particular represent light intensity received by the photodetectors, to an external device.
In another example embodiment of the sensor device, the control and evaluation section, the optics section, and the cavity are contained in a common housing. The housing is closed on one side by the membrane. The housing may in particular be provided with means for connecting the sensor device to a port provided in a vessel. The vessel may for example be a bio-reactor. The port in the vessel mechanically fixes the housing, the side of the housing closed with the membrane pointing towards the interior of the vessel. The housing may also be provided with an interface serving as a connection for a power supply of the sensor device and/or for communication between the sensor device and an external device. The external device may for example be a process control unit, controlling operation of the sensor device, or a data logging device, without being limited thereto. In particular, signals processed by the control and evaluation section may be communicated to the external device via the interface. A further possibility is that calibration data for the sensor device are transferred via the interface to a memory forming part of the control and evaluation section. In particular, a portion of the calibration data required for the signal processing performed by the control and evaluation section may be communicated via the interface to the memory forming part of the control and evaluation section, while a portion of the calibration data required for the further processing of the data received from the sensor device by an external device may be stored in the external device.
In another example embodiment of the sensor device, a portion including the cavity with the membrane is detachable from the optics section. In this case, the cavity will usually be closed on one side by a transparent element, which remains at the cavity when the portion including the cavity is detached from the optics section. The reservoir may be detached along with the portion including the cavity, to which it remains connected. Additionally or alternatively, the control and evaluation section may be detachable from the optics section. In this way, if one of the portions of the sensor device, i.e. the control and evaluation section, or the optics section, or the portion including the cavity with the membrane fails, it is sufficient to replace the failed portion, making maintenance of the sensor device more economical and, due to the modular structure provided by the portions, also easier. Furthermore, the modular structure also allows quick and easy assembling of a sensor device suitable for a specific measurement application by combining an adequate membrane and cavity portion with a suitable optics section and a suitable control and evaluation section.
For other example embodiments, e.g., those of modular structure just mentioned, it is conceivable that a user of the sensor device is provided calibration data along with the portion including the cavity with the membrane, by, for example, the manufacturer or retailer of the sensor device. The user can communicate the calibration data, or a relevant portion thereof, to the sensor device via the interface, as described above. The calibration data may be provided in any suitable form, for example as clear text, barcode, as data file on a storage medium, like for example a USB stick or a flash card, or via download from a data network. The calibration data may be transferred to the sensor device via the interface using any suitable means, like personal computer, tablet, smartphone, by wired or wireless connection. It may also be possible to connect the storage medium, like USB stick or flash card, directly to the sensor device.
The at least one first dye in the membrane may be contained in the membrane in various forms. For example, the at least one first dye can be distributed homogeneously in the membrane or in a layer of the membrane. The at least one first dye also may, within the membrane, be enclosed in hollow particles. In case of more than one first dye, there may be plural types of hollow particles, one type of hollow particle per first dye. A hollow particle containing a dye may contain further substances for establishing a chemical environment required by the dye for proper operation in measurements. A hollow particle may for example contain a buffer in addition to the dye. This buffer may be different from the buffer in the cavity. The hollow particles have a shell enclosing the interior of the particle; the hollow particles may in particular be micelles.
Alternatively, the at least one first dye may be contained within pores in the membrane. As a further alternative, carrier particles may be dispersed in the membrane, and the at least one first dye is absorbed or dissolved in the carrier particles or adsorbed to the carrier particles. Just as in the case of hollow particles, if there is more than one first dye, there may be plural types of carrier particles, one type of carrier particle per first dye. Yet another alternative is that the at least one first dye itself forms particles within the membrane.
In addition, combinations of the above possibilities may be used. For example, one dye may be enclosed in hollow particles, while another dye is absorbed in carrier particles.
In the sensor device according to the invention, the membrane limits the cavity. This means that there is one side of the membrane facing the cavity, and a side of the membrane facing away from the cavity. In an advantageous example embodiment, the side of the membrane facing away from the cavity is opaque. This prevents light directed towards the dyes from escaping from the sensor device and entering a medium containing the analytes to be measured. In this way problems arising from the unintended effects of this light in the medium are avoided, like the unintended excitation of luminescent substances that may be present in the medium. A related problem, equally avoided, is that light from one sensor device disturbs the operation of a further sensor device used at the same time in the medium. The further sensor device may be a sensor device according to the invention or may be of a different type. Furthermore avoided is that any light from outside the sensor device enters the sensor device through the membrane. In particular, if used in combination with an opaque housing, an opaque membrane as described above ensures that only light from the dyes in the sensor device reaches the optics section.
In another example embodiment the cavity contains a spacer element. The spacer element may for example be a porous and at least semi-transparent mat or mesh, woven or non-woven, of, for example, cellulose, plastic, or stainless steel. The spacer element can be used to guarantee a defined distance between the transparent element limiting the cavity and the side of the cavity opposite the transparent element during assembly of the sensor device. Furthermore, the spacer element influences diffusion through the cavity, and therefore also through the spacer element itself, in particular diffusion of buffer and second dye. For reducing the effects of sensor poisoning mentioned above and at the same time allowing a proper function of the sensor device for measurement of the second analyte, the membrane and the spacer element in embodiments are selected in such a way that the diffusion coefficient for the diffusion of the second analyte through the membrane is higher by a factor of 10 to 100 than the diffusion coefficient of the buffer and the second dye in the cavity through the spacer element. The second analyte must be able to change the pH-value of the buffer in the cavity sufficiently for performing measurements of the second analyte with the desired accuracy, which would be difficult or impossible, if changes of the pH-value in the cavity were equilibrated by a fast diffusion process of fresh buffer and second dye from the reservoir into the cavity and through the cavity.
The membrane, in example embodiments, has a layered structure, including a mesh sandwiched between two fluoropolymer films. A non-limiting example of a fluoropolymer is polytetrafluoroethylene (PTFE). Additionally, the mesh between the fluoropolymer films may be embedded in silicone.
These and other objects, features, and advantages of the present disclosure will become readily apparent upon a review of the following detailed description of the disclosure, in view of the drawings and appended claims.
Various embodiments are disclosed, by way of example only, with reference to the accompanying schematic drawings in which corresponding reference symbols indicate corresponding parts, in which:
At the outset, it should be appreciated that like drawing numbers on different drawing views identify identical, or functionally similar, structural elements. It is to be understood that the claims are not limited to the disclosed aspects.
Furthermore, it is understood that this disclosure is not limited to the particular methodology, materials and modifications described and as such may, of course, vary. It is also understood that the terminology used herein is for the purpose of describing particular aspects only, and is not intended to limit the scope of the claims.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure pertains. It should be understood that any methods, devices or materials similar or equivalent to those described herein can be used in the practice or testing of the example embodiments.
It should be appreciated that the term “substantially” is synonymous with terms such as “nearly,” “very nearly,” “about,” “approximately,” “around,” “bordering on,” “close to,” “essentially,” “in the neighborhood of,” “in the vicinity of,” etc., and such terms may be used interchangeably as appearing in the specification and claims. It should be appreciated that the term “proximate” is synonymous with terms such as “nearby,” “close,” “adjacent,” “neighboring,” “immediate,” “adjoining,” etc., and such terms may be used interchangeably as appearing in the specification and claims. The term “approximately” is intended to mean values within ten percent of the specified value.
Adverting now to the figures,
When measuring at least one first analyte and a second analyte in a medium, membrane 1 is brought into contact with the medium, the at least one first analyte and the second analyte enter membrane 1 by diffusion, and subsequently at least the second analyte passes from membrane 1 into cavity 2 by diffusion. The at least one first analyte affects an optical behaviour of a corresponding at least one first dye 11 in membrane 1, and the second analyte affects an optical behaviour of second dye 22 in cavity 2 via a change of the pH-value of buffer 21. The optical behaviour of the at least one first dye 11 and second dye 22 can be monitored by shining light through transparent element 23 on the at least one first dye 11 and second dye 22, which results in light emitted from, or having interacted with, at least one of the at least one first dye 11 or second dye 22, which can pass through transparent element 23 to be registered for evaluation by adequate means. Such means are not shown here. Although such means are not required to form part of a sensor device according to the invention, some example embodiments, for example the embodiment shown in
Sensor device 100 may for example be used to detect gases like sulphur dioxide (SO2), ammonia (NH3), oxygen (O2), or carbon dioxide (CO2). For the detection of oxygen, for example, platinum octaethylporphyrin may be used as a first dye, attached to polystyrene nanoparticles embedded in the membrane. An example of a second dye is hydroxypyrenetrisulfonic acid, and it may be employed in a bicarbonate buffer for the detection of carbon dioxide. Another example for a buffer may be a solution of sodium bisulfate. Additionally, when setting a desired osmolality in the buffer, sodium sulfate may be used. Neither this example nor the invention is limited to the dyes, buffers, and osmolality setting additives just mentioned. Further examples of suitable dyes, and, where applicable, adequate buffers to be used with the dyes, as well as of additives suitable for setting a desired osmolality in a respective buffer, are known in the art for measuring a wide range of analytes.
Reservoir 3 here is formed as an annular recess in transparent element 23. Reservoir 3 is partially covered with opaque layer 32. Opaque layer 32 prevents the contents of reservoir 3, i.e. buffer and second dye (not indicated here), from undesired exposure to light, thus increasing the lifetime of sensor device 100 and preventing a strong hysteresis of the sensor signal, as has already been discussed above. Cavity 2 contains spacer element 24, which at the same time limits reservoir 3 in such a way that reservoir 3 remains in diffusive contact with cavity 2. Spacer element 24, in addition to examples mentioned elsewhere in the application, may for example be a woven or non-woven steel or nylon mesh or PETE-mesh, these examples being independent of the specific embodiment of sensor device 100. In the embodiment shown in
Reservoir 3 of annular shape assists in establishing chemically more homogeneous conditions in cavity 2 via diffusive exchange of buffer and second dye between cavity 2 and reservoir 3. However, the invention is not limited to reservoirs of annular shape. In embodiments like those shown in
In another example embodiment, films 14 and 15 each have a thickness of 5 μm, mesh 16 is a steel mesh of 80 μm layer thickness with a 60 μm mesh size, spacer element 24 also is a steel mesh of 80 μm layer thickness with a 60 μm mesh size. These dimensions are in no way limiting to the invention.
Optics section 50 has one dichroic mirror 51 for each first dye 11 contained in membrane 1. Dichroic mirror 51 here is associated with photodetector 53. Only one dichroic mirror 51 with associated photodetector 53 is shown. Additionally, brackets 110 with subscript “n” are provided, which indicate that this combination of elements may be present in sensor device 100 repeatedly, once for each first dye 11 in membrane 1. Dichroic mirror 51 exhibits a wavelength-dependent reflectivity which is chosen such that dichroic mirror 51 reflects light from the corresponding first dye 11 in membrane 1 to the associated photodetector 53. Photodetector 53 outputs an electric signal indicative of the light intensity received by it. Thus the optical behaviour of first dye 11 can be monitored.
Optics section 50 furthermore has dichroic mirror 52 corresponding to second dye 22 contained in cavity 2. Dichroic mirror 52 here is associated with photodetector 54. Dichroic mirror 52 exhibits a wavelength-dependent reflectivity which is chosen such that dichroic mirror 52 reflects light from the corresponding second dye 22 in cavity 2 to the associated photodetector 54. Photodetector 54 outputs an electric signal indicative of the light intensity received by it. Thus the optical behaviour of second dye 22 can be monitored.
In the example embodiment shown in
Control and evaluation section 70, in the embodiment shown in
Optics section 50 may contain further optical elements. As non-limiting examples thereof, lenses 62 and 63 are shown. For example, lens 62 may be used to collect light emitted by LEDs 61 and shape it into a beam directed towards lens 63, which focuses the light into waveguide 33. Housing 80 may contain further elements. For example, a thermistor (e.g. NTC, Pt100, Pt1000) may be provided in housing 80 for measuring the temperature and thus determine a parameter of the ambient conditions sensor device 100 is used in. Knowledge of the ambient conditions may contribute to the accuracy of the measurement results obtained with sensor device 100, as, for example, the ambient conditions, in particular temperature, can affect a calibration of sensor device 100. In other example embodiments, photodetectors 53 and 54, associated with dichroic mirrors 51 and 52, respectively, may in addition be covered with filters 58, to more precisely define the wavelength range of light reaching respective photodetectors 53 and 54. For more precisely defining the wavelength range of excitation light, one or more filters 64 with a respective suitable transmission may be provided for light source 60, for example one filter for each LED 61, where LEDs 61 can be controlled independently of each other, so that excitation light of different wavelength ranges may be used, depending on requirements of the measuring task.
Sensor device 100, including in particular plug 81, may be sterilisable, for example in an autoclave. Plug 81, without being limited thereto, may be an Interconnex VP6 or VP8.
Control and evaluation section 70, optics section 50, and the portion including membrane 1, cavity 2, reservoir 3, and transparent element 23 here are shown separate from each other, in order to emphasize the modular configuration of the embodiment of sensor device 100 according to the invention shown here. For conducting a measurement, control and evaluation section 70, optics section 50, and a portion including membrane 1, cavity 2, reservoir 3, and transparent element 23 may be selected, according to their respective suitability for the specific measurement to be performed. In the resulting sensor device 100, the portion including membrane 1, cavity 2, reservoir 3, and transparent element 23 is in optical contact with optics section 50, and photodetectors 53 and 54 are in electrical contact with control and evaluation section 70, for example as shown in
External device 90 can, for example, receive signals processed by control and evaluation section 70 (see
Apart from providing a communication or data link between external device 90 and sensor device 100, interface 94, cable 95, and interface 81 may also be used for power supply of sensor device 100. Alternatively, of course, power may be supplied to sensor device 100 in a different way, for example by a separate power line. Wireless communication between sensor device 100 and external device 90 may also be used in example embodiments. User interface 93 can have further purposes, like for example starting, and setting parameters for, one or plural measurements to be conducted with sensor device 100. To the extent necessary, such parameters may also be communicated to sensor device 100, in the example embodiment shown in
It will be appreciated that various aspects of the disclosure above and other features and functions, or alternatives thereof, may be desirably combined into many other different systems or applications. Various presently unforeseen or unanticipated alternatives, modifications, variations, or improvements therein may be subsequently made by those skilled in the art which are also intended to be encompassed by the following claims.
This application is filed under 35 U.S.C. § 111(a) and § 365(c) as a continuation of International Patent Application No. PCT/IB2016/050643, filed Feb. 8, 2016, which application is incorporated herein by reference in its entirety.
| Number | Date | Country | |
|---|---|---|---|
| Parent | PCT/IB2016/050643 | Feb 2016 | US |
| Child | 16039034 | US |
