This invention relates to an optical system and method for concurrently producing differently focused images of an object. The invention has particular application in the formation of images required to produce a phase image of the object. The invention may be embodied in a camera for producing a phase image of an object.
The phase image of an object can be calculated from the information contained within a series of intensity images, captured by a camera, of the object. This series of images is usually termed a “through-focal series” due simply to the arrangement of the intensity images at various small distances from the object's in-focus image in the direction of the light's propagation from the object itself. The process by which this calculation is performed is disclosed in International Patent Application No. PCT/AU99/00949 (Publication No. WO 00/26622) owned by The University of Melbourne, and International Patent Application No. PCT/AU02/0001398 owned by the present applicant. The content of these specifications are incorporated in this specification by this reference.
As described in the above patent applications, the method by which this through-focal series is formed is sequential in nature. Namely, the camera mechanism captures each image of the series one after the other with a small displacement in the image sensor's distance relative to the object occurring between each exposure. Since the displacement of the sensor is typically performed by mechanical means, a measurable period of time will elapse between image exposures. For many applications where the object can be considered stationary (or otherwise static), this time lapse is perfectly acceptable. However, there are numerous applications where the subject of interest moves or otherwise changes its physical appearance at a speed sufficiently fast to render the sequential imaging approach unusable. Cases where this can occur are during the observation of growth or other changes in living cells, the isolation of surface structure of moving objects on a production line, tracking atmospheric changes caused by aircraft or identifying and tracking moving camouflaged vehicles and personnel on a battlefield.
Concurrent imaging camera systems are generally available for high quality colour imaging applications. The mechanism by which the images are captured is with a dichroic beam-splitting prism that accepts an input beam of light from a lens assembly. The input beam is then split, by the prism, into three or more beams, each of a different colour, that are directed towards three or more output windows within the prism. At each of the output windows is located an imaging sensor, typically a CCD array, which create individual images of different colours (
The splitting performed by the prism is typically achieved by several thin film coatings, each of which preferentially reflect a different range of colours. Such coatings are known as dichroic reflectors (
In a first aspect, the present invention provides a system for producing at least two differently focused images of an object, including:
Thus, the invention enables the two sensors to produce the through-focal series previously described. This therefore enables the simultaneous capture of two differently focused images of an object.
In the preferred embodiment of the invention, the beam splitting means comprises a prism.
In one embodiment, the prism includes dichroic beam splitting elements which split the beam into at least two beams, each of a different colour.
However, in another embodiment, the prism may include neutral density filters so that the beam is split into the plurality of resultant beams, each of which exhibits no preferential colouration.
The level of light transmission through each of the neutral density filters will depend on the number of sensors being used. Typically for three sensors, a first filter would reflect 33% and transmit 67% of the incident beam, whilst a second filter would transmit and reflect 50% of the beam from the first filter. In this way, each sensor would receive 33% of the original incident beam.
Preferably, the sensors comprise CCD arrays. However, in other embodiments, the sensors could comprise photo diodes or the like. Photo diodes have particular application in environments in which the optical system is used in a confocal microscope which scans across an object in order to produce an image.
The beam of radiation is preferably electromagnetic radiation of any desired wavelength, including infrared, visible light, ultraviolet and X-rays. However, the beam could also be particle radiation, such as an electron beam, and mechanical radiation, such as acoustic waves.
In one embodiment, the sensors are located at different distances from respective exit points of the resultant beams from the beam splitting means to thereby produce the different path lengths. However, in another embodiment, the beam splitting means is longer or shorter in the direction of the respective resultant beam to the respective sensor, and the sensors are attached directly to the beam splitting means to thereby create the different path lengths.
In a still further embodiment, the different path lengths are provided by the location of optical elements between the beam splitting means and the sensors, so as to create a different path length of the resultant beam from the beam splitting means to the respective sensor.
In the preferred embodiment of the invention, the element comprises a pair of transparent wedge-shaped members which are movable relative to one another so as to alter the amount of the wedge through which the resultant beam passes to thereby change the path length of the resultant beam to produce the different path lengths. In this embodiment, the sensors are located at equal distances from the beam splitting means.
In one embodiment of the invention, a beam conditioning element is located between the beam splitting means and the respective sensor.
Preferably, a plurality of beam conditioning elements are locatable between the beam splitting means and the sensors, and moving means is provided for moving the elements, such as to bring one of the elements in turn into registry with the respective sensor so the resultant beam passes through the said one of the elements. In this way, the moving means can move any one of the elements into alignment so as to produce the required conditioning of the beam prior to detection by the sensor.
The conditioning elements may include colour imaging filters, a de-focus wedge system comprised of a pair of transparent wedge elements, and a polariser.
In one embodiment of the invention, the beam comprises an electron beam, and the beam splitting means comprises a plurality of sensors arranged along the direction of the path of the electron beam, and wherein some of the electron beam is detected by a first of the sensors and some of the beam passes through the first of the sensors to a subsequent sensor for detection by that sensor to thereby produce the different path lengths.
In a second aspect, the invention may be said to reside in a system for producing differently focused images of an object, including:
In the preferred embodiment of the invention, the beam splitting means comprises a prism. In one embodiment, the prism includes dichroic beam splitting elements which split the beam into at least two beams, each of a different colour.
However, in another embodiment, the prism may include neutral density filters so that the beam is split into the plurality of resultant beams, each of which exhibits no preferential colouration.
The level of light transmission through each of the neutral density filters will depend on the number of sensors being used. Typically for three sensors, a first filter would reflect 33% and transmit 67% of the incident beam, whilst a second filter would transmit and reflect 50% of the beam from the first filter. In this way, each sensor would receive 33% of the original incident beam.
Preferably, the sensors comprise CCD arrays. However, in other embodiments, the sensors could comprise photo diodes or the like. Photo diodes have particular application in environments in which the optical system is used in a confocal microscope which scans across an object in order to produce an image.
The beam of radiation is preferably electromagnetic radiation of any desired wavelength, including infrared, visible light, ultraviolet and X-rays. However, the beam could also be particle radiation, such as an electron beam, and mechanical radiation, such as acoustic waves.
In the preferred embodiment of the invention, the element comprises a pair of transparent wedge-shaped members which are movable relative to one another so as to alter the amount of the wedge through which the resultant beam passes to thereby change the path length of the resultant beam to produce the different path lengths. In this embodiment, the sensors are located at equal distances from the beam splitting means.
In one embodiment of the invention, a beam conditioning element is located between the beam splitting means and the respective sensor.
Preferably, a plurality of beam conditioning elements are locatable between the beam splitting means and the sensors, and moving means is provided for moving the elements, such as to bring one of the elements in turn into registry with the respective sensor so the resultant beam passes through the said one of the elements. In this way, the moving means can move any one of the elements into alignment so as to produce the required conditioning of the beam prior to detection by the sensor.
The conditioning elements may include colour imaging filters.
A third aspect of the invention may be said to reside in a system for producing differently focused images of an object, including:
In the preferred embodiment of the invention, the beam splitting means comprises a prism. In one embodiment, the prism includes dichroic beam splitting elements which split the beam into at least two beams, each of a different colour.
However, in another embodiment, the prism may include neutral density filters so that the beam is split into the plurality of resultant beams, each of which exhibits no preferential colouration.
The level of light transmission through each of the neutral density filters will depend on the number of sensors being used. Typically for three sensors, a first filter would reflect 33% and transmit 67% of the incident beam, whilst a second filter would transmit and reflect 50% of the beam from the first filter. In this way, each sensor would receive 33% of the original incident beam.
Preferably, the sensors comprise CCD arrays. However, in other embodiments, the sensors could comprise photo diodes or the like. Photo diodes have particular application in environments in which the optical system is used in a confocal microscope which scans across an object in order to produce an image.
The beam of radiation is preferably electromagnetic radiation of any desired wavelength, including infrared, visible light, ultraviolet and X-rays. However, the beam could also be particle radiation, such as an electron beam, and mechanical radiation, such as acoustic waves.
In one embodiment, the sensors are located at different distances from respective exit points of the resultant beams from the beam splitting means to thereby produce the different path lengths. However, in another embodiment, the beam splitting means is longer or shorter in the direction of the respective resultant beam to the respective sensor, and the sensors are attached directly to the beam splitting means to thereby create the different path lengths.
In a still further embodiment, the different path lengths are provided by the location of optical elements between the beam splitting means and the sensors, so as to create a different path length of the resultant beam from the beam splitting means to the respective sensor.
In the preferred embodiment of the invention, the element includes a pair of transparent wedge-shaped members which are movable relative to one another so as to alter the amount of the wedge through which the resultant beam passes to thereby change the path length of the resultant beam to produce the different path lengths.
The conditioning elements may include colour imaging filters and a polariser.
The invention may also be said to reside in a method of producing differently focused images of an object, including:
In the preferred embodiment of the inventions referred to above, the differently focused images are comprised of at least one negatively focused image, an in-focus image, and at least one positively focused image. In this embodiment, three sensors are provided and the beam splitting means splits the radiation into three resultant beams, each for detection by one of the sensors.
In a still further aspect of the invention, motion or movement detection is contemplated.
In one embodiment, the system may comprise any of the systems described previously, and the images received by the sensors are time delayed with respect to one another so at least two images which are time delayed are detected by the sensors. These images can then be compared with one another to determine whether there has been any movement of the object to determine motion of the object. The time delay can be provided by taking the images sequentially, rather than concurrently as described above, or producing a time delay by causing one beam to travel along a relatively lengthy path, and another beam to travel along a much shorter path so that the images can be captured concurrently on the different sensors whilst still providing two images which are time delayed for comparison to determine motion. The time delay image can be provided by causing one of the beams to pass through a significant length of optical fibre or the like.
This aspect of the invention may also be said to reside in a system for determining movement of an object, including:
Preferably, the images comprise phase images of the object.
Preferably, the comparison is made by the processing means based on a difference between the images.
The comparison of the images and the determination of whether the object has moved may be performed by a single processing means.
In the preferred embodiment of this aspect of the invention, the determination of whether the object has moved is made by creating a phase image of the object from the images which are captured by the sensor and inspecting the phase image to observe light and dark shadows on details in the image, and thereby determining whether the object is moving towards or away from the sensor.
This aspect of the invention may also be said to reside in a method of determining movement of an object, including:
Preferred embodiments of the invention will be described, by way of example, with reference to the accompanying drawings, in which:
With reference to
In the preferred embodiment, the optical system shown in
As shown in
Thus, according to the embodiment of
In a second embodiment shown in
In these embodiments, to ensure no time lapse during the capture of the individual images, each of the image sensors must be triggered at precisely the same time. This triggering method is a standard procedure in existing commercially available 3-CCD cameras. However, it should be noted that in some embodiments, as will be explained in more detail, a short delay between the images may be advantageous. These embodiments include the highlighting of turbulent airflow, and also systems which are used for determining movement of an object.
It should be noted that this embodiment can also be arranged in the same manner as
The configuration shown in
In embodiments which use acoustic waves, the beam splitter could be in the form of a member or beam splitter based on a refractive mismatch which provides for amplitude splitting, and the sensors could be ultra sonic transducers.
With reference to
Since this technique requires the capture of images which are sequential in time rather than concurrent in time, only a single sensor need be used as is shown in
Since modifications within the spirit and scope of the invention may readily be effected by persons skilled within the art, it is to be understood that this invention is not limited to the particular embodiment described by way of example hereinabove.
Number | Date | Country | Kind |
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2004901223 | Mar 2003 | AU | national |
Filing Document | Filing Date | Country | Kind | 371c Date |
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PCT/AU05/00204 | 2/17/2004 | WO | 8/30/2006 |