Optical waveguide bio-sensing device

Abstract

An optical waveguide bio-sensing device, comprising: a monochromatic light source, a beam splitter, a grating chip with a sub-wavelength grating structure and a sensor. Moreover, in order to enhance the sensitivity of the system using the optical waveguide bio-sensing device, a light concentrating element or a quarter waveplate (¼λ) is arranged in the optical path during detection. When the grating chip is not coated with a layer of biochemical substance corresponding to a monochrome light emitted from the monochromatic light source, a reflected light of a specific narrow wavelength is reflected by a specific angle as a surface plasmon resonant effect caused by waveguide coupling is excited by the incidence of the monochrome light to the grating chip through the beam splitter; otherwise, there will be little or no reflection. Accordingly, the optical waveguide bio-sensing device can detect bio-molecular interactions, reaction rate and/or molecular dynamics without any labeling in real time. Optical systems using the present invention are simplified and capable of being portable. Thus a high sensitivity detection technique is achieved.

Description

BRIEF DESCRIPTION OF THE DRAWINGS

The objects, spirits and advantages of the preferred embodiments of the present invention will be readily understood by the accompanying drawings and detailed descriptions, wherein:

FIG. 1 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is not bounded with any bio-molecule according to a first embodiment of the present invention;

FIG. 1A shows the relation between the wavelength λ and the reflectance;

FIG. 2 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is bounded with bio-molecules according to the optical waveguide bio-sensing device in FIG. 1;

FIG. 2A shows the relation between the wavelength λ+Δλ and the reflectance after bio-molecular bounding;

FIG. 3 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is not not bounded with any bio-molecule according to a second embodiment of the present invention;

FIG. 3A shows the relation between the wavelength λ and the reflectance;

FIG. 4 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is bounded with bio-molecules according to the optical waveguide bio-sensing device in FIG. 3;

FIG. 4A shows the relation between the wavelength λ+Δλ and the reflectance after bio-molecular bounding;

FIG. 5 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is not bounded with any bio-molecule according to a third embodiment of the present invention;

FIG. 5A shows a cross-sectional view of a Fresnel surface;

FIG. 6 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is bounded with bio-molecules according to the optical waveguide bio-sensing device in FIG. 5;

FIG. 7 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is not not bounded with any bio-molecule according to a fourth embodiment of the present invention;

FIG. 8 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is bounded with bio-molecules according to the optical waveguide bib-sensing device in FIG. 7;

FIG. 9 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is not not bounded with any bio-molecule according to a fifth embodiment of the present invention;

FIG. 10 is a schematic diagram showing an optical waveguide bio-sensing device wherein the grating chip is bounded with bio-molecules according to the optical waveguide bio-sensing device in FIG. 9;

FIG. 11 is a schematic diagram showing an optical waveguide bio-sensing device according to a sixth embodiment of the present invention; and

FIG. 11A is a schematic diagram showing an optical waveguide bio-sensing device according to a seventh embodiment of the present invention.

Claims

1. An optical waveguide bio-sensing device, comprising: a light source, capable of emitting a monochromatic light;a grating chip, comprising at least a grating structure and a waveguide layer on the grating structure for receiving the monochromatic light, wherein a receptor is provided on the waveguide layer;a beam splitter, disposed between the light source and the grating chip for the monochromatic light to transmit therethrough or to be reflected thereby to generate a reflected light; anda sensor, for detecting the reflected light.

2. The optical waveguide bio-sensing device as recited in claim 1, wherein the light source is a laser source.

3. The optical waveguide bio-sensing device as recited in claim 1, wherein the light source comprises at least a light emitting element.

4. The optical waveguide bio-sensing device as recited in claim 1, wherein the waveguide layer comprises at least one of silicon nitride, gallium nitride, tantalum oxide, indium-tin oxide, indium- gallium arsenide, gallium arsenide, indium phosphide, gallium arsenide-antimonide, magnesium fluoride, zinc sulfide, zinc telluride, beryllium-zinc telluride, magnesium selenide, aluminum-gallium nitride, aurum, argentum, sulfhydryl (HS), amine (NH2), aldehyde (CHO), carboxyl (COOH), biotin and combination thereof.

5. The optical waveguide bio-sensing device as recited in claim 1, wherein the grating chip comprises grating structures on both the top surface and the bottom surface.

6. The optical waveguide bio-sensing device as recited in claim 1, further comprising a light concentrating element.

7. The optical waveguide bio-sensing device as recited in claim 6, wherein the light concentrating element is disposed between the light source and the beam splitter, between the grating chip and the beam splitter, between the beam splitter and the sensor, or on the beam splitter.

8. The optical waveguide bio-sensing device as recited in claim 6, wherein the light concentrating element is a concentrating lens or an equivalent lens with a Fresnel surface.

9. The optical waveguide bio-sensing device as recited in claim 1, further comprising a quarter waveplate.

10. The optical waveguide bio-sensing device as recited in claim 9, wherein the quarter waveplate is disposed between the light source and the beam splitter, between the grating chip and the beam splitter, or on the beam splitter.

11. An optical waveguide bio-sensing device, comprising: a light source, capable of emitting a monochromatic light;a grating chip, comprising at least a grating structure and a waveguide layer on the grating structure for receiving the monochromatic light, wherein a receptor is provided on the waveguide layer; anda sensor, disposed opposite to the light source with the grating chip in-between for detecting the monochromatic light transmitting through the grating chip.

12. The optical waveguide bio-sensing device as recited in claim 11, wherein the light source is a laser source.

13. The optical waveguide bio-sensing device as recited in claim 11, wherein the light source comprises at least a light emitting element.

14. The optical waveguide bio-sensing device as recited in claim 11, wherein the waveguide layer comprises at least one of silicon nitride, gallium nitride, tantalum oxide, indium-tin oxide, indium-gallium arsenide, gallium arsenide, indium phosphide, gallium arsenide-antimonide, magnesium fluoride, zinc sulfide, zinc telluride, beryllium-zinc telluride, magnesium selenide, aluminum-gallium nitride, aurum, argentum, sulfhydryl (HS), amine (NH2), aldehyde (CHO), carboxyl (COOH), biotin and combination thereof.

15. The optical waveguide bio-sensing device as recited in claim 11, wherein the grating chip comprises grating structures on both the top surface and the bottom surface.

16. The optical waveguide bio-sensing device as recited in claim 11, further comprising a light concentrating element.

17. The optical waveguide bio-sensing device as recited in claim 16, wherein the light concentrating element is disposed between the light source and the grating chip, or between the grating chip and the sensor.

18. The optical waveguide bio-sensing device as recited in claim 16, wherein the light concentrating element is a concentrating lens or an equivalent lens with a Fresnel surface.

19. An optical waveguide bio-sensing device, comprising: a light source, capable of emitting a monochromatic light;a grating chip, comprising at least a grating structure and a waveguide layer on the grating structure for receiving the monochromatic light, wherein a receptor is provided on the waveguide layer; anda sensor, disposed on the same side of the grating chip as the light source for detecting a reflected light reflected by the grating chip.

20. The optical waveguide bio-sensing device as recited in claim 19, wherein the light source is a laser source.

21. The optical waveguide bio-sensing device as recited in claim 19, wherein the light source comprises at least a light emitting element.

22. The optical waveguide bio-sensing device as recited in claim 19, wherein the waveguide layer comprises at least one of silicon nitride, gallium nitride, tantalum oxide, indium-tin oxide, indium-gallium arsenide, gallium arsenide, indium phosphide, gallium arsenide-antimonide, magnesium fluoride, zinc sulfide, zinc telluride, beryllium-zinc telluride, magnesium selenide, aluminum-gallium nitride, aurum, argentum, sulfhydryl (HS), amine (NH2), aldehyde (CHO), carboxyl (COOH), biotin and combination thereof.

23. The optical waveguide bio-sensing device as recited in claim 19, wherein the grating chip comprises grating structures on both the top surface and the bottom surface.

24. The optical waveguide bio-sensing device as recited in claim 19, further comprising a light concentrating element.

25. The optical waveguide bio-sensing device as recited in claim 24, wherein the light concentrating element is disposed between the light source and the grating chip, or between the grating chip and the sensor.

26. The optical waveguide bio-sensing device as recited in claim 24, wherein the light concentrating element is a concentrating lens or an equivalent lens with a Fresnel surface.

27. The optical waveguide bio-sensing device as recited in claim 19, further comprising a quarter waveplate.

28. The optical waveguide bio-sensing device as recited in claim 27, wherein the quarter waveplate is disposed between the light source and the grating chip, or between the grating chip and the sensor.