Patent Application
20080242698
This invention relates to the use of diamines of formula I for the manufacture of pharmaceutical preparations for the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission and of nervous system disorders regulated full or in part by mGluR7, novel diamines of formula I, methods of their preparation and pharmaceutical compositions containing them.
Surprisingly, it has been found that the diamines of formula I have advantageous pharmacological properties and are suitable, for example, for the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission and of nervous system disorders regulated full or in part by mGluR7.
Hence, in a first aspect, the present invention relates to the use of a diamine of formula I
wherein
The general terms used hereinbefore and hereinafter preferably have within the context of this disclosure the following meanings, unless otherwise indicated:
Where the plural form is used for compounds, salts, and the like, this is taken to mean also a single compound, salt, or the like.
Alkyl is especially alkyl with from and including 1 up to and including 7, preferably from and including 1 to and including 4, C atoms and is linear or branched; preferably, alkyl is methyl, ethyl, propyl, such as n-propyl or isopropyl, butyl, such as n-butyl, sec-butyl, iso-butyl or tert-butyl.
Alkoxy is especially alkyl with from and including 1 up to and including 7, preferably from and including 1 to and including 4, C atoms and is linear or branched; preferably, alkoxy is methoxy, ethoxy or propoxy, e.g. n-propoxy.
Alkenyl is especially alkenyl with from and including 1 up to and including 7, preferably from and including 1 to and including 4, C atoms and is linear or branched and bound to the molecule via a carbon atom having only single bonds, i.e. which is sp3-hybridized; preferably, alkenyl is allyl.
Halogen or halo is especially fluorine, chlorine, bromine, or iodine, especially fluorine, chlorine, or bromine.
Aryl is a monocyclic, bicyclic or tricyclic aromatic radical having 4 to 14 carbon atoms, in particular 4 to 12, preferably 6 to 12 carbon atoms, being fully unsaturated or partially saturated. In a preferred embodiment, aryl is especially phenyl, fluorenyl, indanyl, acenaphthylenyl, 10,11-dihydro-5H-dibenzo[a,d]cycloheptenyl, tetrahydronaphthyl or naphthyl.
Heterocyclyl containing oxygen, sulfur or nitrogen as defined herein denotes a mono-, bi-, tri- or tetracyclic heterocyclic system with 1 or 2 heteroatoms especially selected from nitrogen, oxygen, and sulfur, which may be unsaturated or wholly or partly saturated, and is preferably thiazolyl, especially thiazol-5-yl, benzo[b]furyl, especially benzo[b]furan-6-yl, benzoxazolyl, especially benzoxazol-2-yl, pyridyl, especially 2-pyridyl, pyrimidyl, indolyl, especially indol-6-yl, quinolinyl, dihydro-quinolinyl, especially 3,4-dihydro-2H-quinolin-1-yl, isoquinolinyl, dihydro-isoquinolinyl, especially 3,4-dihydro-1H-isoquinolin-2-yl, phenoxazinyl, especially phenoxazin-10-yl, carbazolyl, especially 1-carbazol-9-yl, dibenzo[b,f]azepinyl, especially dibenzo[b,f]azepin-5-yl, dihydro-dibenzo[b,f]azepinyl, especially 10,11-dihydro-dibenzo[b,f]azepin-5-yl, chromanyl, e.g. 2H-chromanyl, especially 2H-chroman-4-yl, dihydro-chromanyl, e.g. 3,4-dihydro-2H-chromanyl, especially 3,4-dihydro-2H-chroman-4-yl, piperidinyl, such as piperidin-1-yl or piperidin-4-yl, 5,6,11,12-tetrahydro-dibenzo[a,e]cyclo-octen-5,11-iminyl, especially 5,6,11,12-tetrahydro-dibenzo[a,e]cycloocten-5,11-imine-14-yl, 10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-iminyl, benzo[1,4]dioxinyl, e.g. 2,3-dihydro-benzo[1,4]dioxinyl, especially 2,3-dihydro-benzo[1,4]dioxin-2-yl or thieno-pyrimidyl, e.g. thieno[2,3-d]-pyrimidyl, especially thieno[2,3-d]-pyrimidin-4-yl.
Cycloalkyl is especially (C3-C8)cycloalkyl, namely cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptenyl or cyclooctyl, preferably cyclopropyl.
Hetaryl refers to a heterocyclic moiety that is unsaturated in the ring binding the heteroaryl radical to the rest of the molecule in formula I, where at least in the binding ring, but optionally also in any annealed ring, one or more, preferably 1 to 4 carbon atoms are replaced each by a heteroatom selected from the group consisting of nitrogen, oxygen and sulfur; such as thienyl, furyl, pyranyl, thianthrenyl, isobenzofuranyl, benzofuranyl, chromenyl, 2H-pyrrolyl, pyrrolyl, imidazolyl, benzimidazolyl, pyrazolyl, thiazolyl, isothiazolyl, oxazolyl, isoxazolyl, pyridyl, pyrazinyl, pyrimidyl, pyridazyl, indolizinyl, isoindolyl, 3H-indolyl, indolyl, indazolyl, triazolyl, tetrazolyl, purinyl, 4H-quinolizinyl, isoquinolyl, quinolyl, phthalazinyl, naphthyridinyl, quinoxaloyl, quinazolinyl, cinnolinyl, pteridinyl, carbazolyl, phenanthridinyl, acridinyl, perimidinyl, phenanthrolinyl, benzo[b]furyl, benzoxazolyl, benzthiazolyl, phenoxazinyl, dibenzo[b,f]azepinyl, chromanyl, benzo[1,4]dioxinyl, or thieno-pyrimidyl. Hetaryl preferably denotes a mono-, bi-, tricyclic heterocyclic system with 1 or 2 heteroatoms consisting of nitrogen, oxygen and sulfur, which is fully unsaturated, and is preferably thiazolyl, especially thiazol-5-yl, benzo[b]furyl, especially benzo[b]furan-6-yl, benzoxazolyl, especially benzoxazol-2-yl, pyridyl, especially 2-pyridyl, pyrimidyl, indolyl, especially indol-6-yl, quinolyl, isoquinolyl, phenoxazinyl, carbazolyl, dibenzo[b,f]azepinyl, chromanyl, e.g. 2H-chromanyl, benzo[1,4]dioxinyl, or thieno-pyrimidyl, e.g. thieno[2,3-d]-pyrimidyl, especially thieno[2,3-d]-pyrimidin-4-yl.
Unless otherwise mentioned, the term “unsubstituted or substituted” as used herein means that the respective radical is unsubstituted or substituted by one or more, preferably up to four, especially one or two substituents, selected from amino, C1-C4alkyl amino, di(C1-C4alky)-amino, hydroxy-C1-C4alkyl amino, phenyl-C1-C4alkyl amino, C3-C5cycloalkyl amino, di(C3-C5)cycloalkyl amino, N—C1-C4alkyl-N—C3-C5cycloalkyl amino, C1-C4alkanoyl amino, halogen, hydroxy, C1-C4alkoxy, perfluoro-C1-C4alkoxy, C3-C6cycloalkyl, C3-C5cycloalkyloxy, C1-C4alkoxy C1-C4alkoxy, di(C1-C4alkyl)-amino C1-C4alkoxy, carbamoyl, N—C1-C4alkyl-carbamoyl, N,N-di(C1-C4alkyl)-carbamoyl, nitro, cyano, carboxy, C1-C4alkoxy carbonyl, C1-C4alkanoyl, C1-C4alkanoyloxy, benzoyl, amidino, guanidino, ureido, mercapto, C1-C4alkylthio, pyridyl, phenyl, phenoxy, phenylthio, phenyl-C1-C4alkylthio, C1-C4alkylsulfonyl, phenyl-sulfonyl, C1-C4alkylphenylsulfonyl, C1-C4alkenyl, C4-C8heterocyclyl, C4-C8heterocyclyloxy, C4-C8heterocyclyl C1-C4alkoxy, C1-C4alkylene dioxy bound at adjacent C-atoms of the ring, and C1-C4alkyl, which is unsubstituted or substituted by halogen, hydroxy, C1-C4alkoxy, nitro, imino, cyano, carboxy, C1-C4alkoxy carbonyl, C1-C4alkanoyl, C1-C4alkanoyloxy or C4-C8heterocyclyl.
On account of the asymmetrical carbon atom(s) present in the diamines of formula I and their salts, the compounds may exist in optically active form or in form of mixtures of optical isomers, e.g. in form of racemic mixtures. All optical isomers and their mixtures including the racemic mixtures are part of the present invention.
In view of the close relationship between the novel compounds in free form and those in the form of their salts, including those salts that can be used as intermediates, for example in the purification or identification of the novel compounds, any reference to the free compounds hereinbefore and hereinafter is to be understood as referring also to the corresponding salts, as appropriate and expedient.
Salts are formed, for example, as acid addition salts, preferably with organic or inorganic acids, from compounds of formula I with a basic nitrogen atom, especially the pharmaceutically acceptable salts. For isolation or purification purposes it is also possible to use pharmaceutically unacceptable salts, for example picrates or perchlorates. For therapeutic use, only pharmaceutically acceptable salts or free compounds are employed (where applicable in the form of pharmaceutical preparations), and these are therefore preferred.
The compounds of formula I, including their acid addition salts, may also be obtained in the form of hydrates or may include the solvent used for crystallization.
In formula I the following significances are preferred independently, collectively or in any combination or sub-combination:
(a) Z is —C(O)— or a bond;
(b) n is 0, 1 or 2;
(c) p is 0 or 1;
(d) R1 denotes (C1-6)alkyl, e.g. methyl, which is unsubstituted or mono- or disubstituted by phenyl, which itself is unsubstituted or substituted by halogen, (C1-6)alkoxy or trifluoromethoxy, phenoxy, 2,3-dihydro-benzo[1,4]dioxanyl or (C5-C7)cycloalkyl; (C1-6)alkenyl; naphthyl, 9H-fluorenyl, 10,11-dihydro-5H-dibenzo[a,d]cycloheptenyl, 2,3-dihydrobenzo[b]furyl, 2,3-dihydrochromenyl; phenyl which is unsubstituted or substituted by phenyl or (C1-6)alkoxy; or piperidinyl which is substituted by pyrimidyl; and R1′ denotes phenyl, hydrogen or (C1-6)alkyl;
(e) alternatively, R1 and R1′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ia, Ib, Ic or Id,
(f) A denotes N and E denotes CR4R4′ or A denotes CR4R4′ and E denotes N,
(g) G denotes CH2,
(h) X represents oxygen, a bond, —CH═CH— or —CH2—CH2—,
(i) m is 0 or 1,
(j) R4 and R4′ represent, independently of each other, hydrogen or (C1-6)alkyl,
(k) R5 represents hydrogen, (C1-6)alkyl, CF3 or halogen,
(l) R6 represents hydrogen or (C1-6)alkyl,
(m) R7 and R8 are both hydrogen,
(n) R9 and R10 are both hydrogen,
(o) R11 is hydrogen or (C1-6)alkoxy,
(p) R12 represents hydrogen,
(q) R13 represents hydrogen or (C1-6)alkyl,
(r) R2 denotes hydrogen, (C1-6)alkyl, benzyl, or phenyl, which is unsubstituted or substituted by halogen,
(s) R3 denotes hydrogen, fluorenyl, 2,3-dihydrochromenyl, 1,2,2a,3,4,5-hexahydro-acenaphthyl, 1,2-dihydro-acenaphthyl, tetrahydronaphthyl which is unsubstituted or substituted by (C1-6)alkoxy; benzoxazolyl; cyano-pyridyl; (C1-6)alkyl thieno[2,3-d]-pyrimidyl; N—(C1-6)alkyl piperidinyl, which is substituted by phenyl; (C3-C4)cycloalkyl which is substituted by phenyl; 2,3-dihydro-indenyl; (C1-6)alkyl which is unsubstituted or substituted by hydroxy, amino, naphthyl, indolyl, thiazolyl, pyridyl or phenyl, which itself is unsubstituted or mono- or disubstituted by halogen, phenyl, nitro, di-(C1-6)alkyl amino, di-(C1-6)alkyl amino (C1-6)alkoxy, (C1-6)alkyl, (C1-6)alkoxy, trifluoromethyl or trifluoromethoxy; or R17—C(O)—, wherein R17 denotes (C1-6)alkyl, phenyl, indolyl or benzo[b]furyl, which in each case is substituted by cyano, carboxy or (C1-6)alkoxy carbonyl;
(t) R3′ denotes hydrogen, (C1-6)alkyl, (C1-6)alkyl carbonyl or benzyl,
(u) Alternatively, R3 and R3′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ie or If,
(v) Q denotes N and T denotes CR16R16′ or Q denotes CR16R16′ and T denotes N,
(w) R14 denotes hydrogen,
(x) R15 denotes hydrogen,
(y) R16 denotes hydrogen,
(z1) R17 denotes hydrogen,
(z2) R16′ denotes hydrogen,
(z3) R18 denotes hydrogen.
In a further aspect, the invention provides processes for the production of the compounds of formula I which comprises the steps as defined below.
a) For the production of a compound of formula I, wherein Z is C(O) and wherein the other radicals and symbols have the meanings as provided for a compound of formula I above, by reacting a halide of formula II
wherein n, p, Z, R1, R1′ and R2 are defined as for a compound of formula I above, and Y is Cl, Br or I with an amine of formula III
wherein R3 and R3′ are defined as for a compound of formula I above.
b) Compounds of formula I, wherein Z is C(O) and wherein the other radicals and symbols have the meanings as provided for a compound of formula I above, can also be prepared by coupling of a carboxylic acid of formula IV
wherein n, p, R3, R3′ and R2 are as defined above for a compound of formula I, with a primary or secondary amine of formula V
wherein R1 and R1′ are as defined above for a compound of formula I.
c) Alternatively, compounds of formula I, wherein Z is C(O), R3 is R17—C(O)—, wherein R17 denotes unsubstituted or substituted alkyl, or R3 is alkyl which is unsubstituted or substituted by hydroxy, amino, unsubstituted or substituted (C4-C12)aryl, unsubstituted or substituted (C4-C12)hetaryl or unsubstituted or substituted aryl, R3′ is hydrogen and the other radicals and symbols have the meanings as provided for a compound of formula I above, can be synthesized by alkylation, reductive alkylation or acylation of the diamine of formula I, wherein R3 and R3′ are both hydrogen, with an alkylating agent, a carbonyl compound or an acylating agent.
d) A compound of formula I, wherein Z is C(O), p is 0 and the other radicals have the meanings as provided for a compound of formula I above, can be prepared by reacting a carbamoyl chloride of formula VI
wherein R1 and R1′ defined as above for a compound of formula I, with a primary or secondary amine of formula III
wherein R3 and R3′ are defined as above for a compound of formula I.
e) A compound of formula I, wherein Z is a bond and the other radicals have the meanings as provided for a compound of formula I above, can be prepared by subjecting a diamine of formula VII
wherein n, p, R1 and R2 are as defined above for a compound of formula I alkylation or acylation known as such to provide diamines of formula I.
The reaction of process a) can be effected according to conventional methods known in the art, e.g. as described in the Examples, e.g. in a suitable solvent such as an alcohol, optionally in the presence of a suitable base, such as potassium carbonate.
The amide formation of process b) can be performed by standard procedures, e.g. as described in the Examples. The reaction can, for instance, be carried out in a suitable solvent in the presence of a base, such as a trialkylamine, optionally a water scavenger, such as dicyclohexyl-carbodiimide or diisopropylcarbodiimide, and, also optionally, hydroxyl-benzotriazol, between room temperature and the reflux temperature of the employed solvent.
Alkylation, reductive alkylation and acylation as described in process c) can be achieved as known in the art. The reductive alkylation can be carried out, e.g., with hydrogenation in the presence of a catalyst, such as platinum or palladium, which is preferably bonded to a carrier material, such as carbon, or a heavy metal catalyst, such as Raney nickel, at normal pressure or at pressures of from 0.1 to 10 MegaPascal (MPa), or with reduction by means of complex hydrides, such as borohydrides, especially alkali metal acetoborohydrides, for example sodium acetoborohydride, in the presence of a suitable acid, preferably relatively weak acids, such as acetic acid, in customary solvents, for example alcohols, such as methanol or ethanol.
Process d) can be carried out between 15° C. and 50° C., especially about 20° C. to about 25° C., in a suitable solvent, such as dichloromethane, optionally in the presence of a suitable base, for a duration between 3 and 36 hours, e.g. about 24 hours.
A diamine of formula VII, wherein n, p, R1 and R2 are as defined above for a compound of formula I used as starting material in process e) can be obtained by the following reaction sequence. A compound of formula I, wherein Z is a bond and the other radicals have the meanings as provided for a compound of formula I above, can be prepared by reacting a diamine of formula VIII
wherein n, p, and R2 are as defined above for a compound of formula I and PG represents a protecting group, is reacted with a halogenide of formula IX,
R1-Hal (IX)
wherein Hal represents halogen, preferably bromo, and R1 has the meaning as defined above for a compound of formula I to furnish a diamine of formula X,
wherein n, p, R1 and R2 are as defined above for a compound of formula I and PG represents a protecting group. Said diamine of formula X is then subjected to reaction conditions under which the protecting group is split off delivering a diamine of formula VII.
All processes a)-d) can also be performed on solid phase using well established methodology, e.g. as described in the Examples.
A so obtained compound of formula I can be converted into another compound of formula I according to conventional methods, e.g. those described in the Examples.
Working up the reaction mixtures according to the above processes and purification of the compounds thus obtained may be carried out in accordance to known procedures.
Acid addition salts may be produced from the free bases in known manner, and vice versa.
Compounds of formula I in optically pure form can be obtained from the corresponding racemates according to well-known procedures. Alternatively, optically pure starting materials can be used.
The starting materials of formulae II-IX are known or may be obtained from known compounds, using conventional procedures, e.g. those described in the Examples.
Compounds of formula I obtained in accordance with the above-described process can also be converted into other compounds of formula I in customary manner, e.g. as described in Examples 114 or 115.
Resulting acid addition salts can be converted into other acid addition salts or into the free bases in a manner known per se.
A compound of formula I, wherein Z is C(O), can be converted into the respective compound wherein Z is C(S), for example, by reaction with Lawesson's reagent (2,4-bis-(4-methoxy-phenyl)2,4-dithioxo-1,2,3,4-dithiaphosphetan) in a halogenated carbon hydrate, such as dichloromethane, or an aprotic solvent, such as toluene, at temperatures from about 30° C. to reflux.
If one or more other functional groups, for example carboxy, hydroxy, amino, or mercapto, are or need to be protected in a compound of formulae II or III, because they should not take part in the reaction, these are such groups as are usually used in the synthesis of peptide compounds, and also of cephalosporins and penicillins, as well as nucleic acid derivatives and sugars.
The protecting groups may already be present in precursors and should protect the functional groups concerned against unwanted secondary reactions, such as acylations, etherifications, esterifications, oxidations, solvolysis, and similar reactions. It is a characteristic of protecting groups that they lend themselves readily, i.e. without undesired secondary reactions, to removal, typically by solvolysis, reduction, photolysis or also by enzyme activity, for example under conditions analogous to physiological conditions, and that they are not pre-sent in the end-products. The specialist knows, or can easily establish, which protecting groups are suitable with the reactions mentioned hereinabove and hereinafter.
The protection of such functional groups by such protecting groups, the protecting groups themselves, and their removal reactions are described for example in standard reference works, such as J. F. W. McOmie, “Protective Groups in Organic Chemistry”, Plenum Press, London and New York 1973, in T. W. Greene, “Protective Groups in Organic Synthesis”, Wiley, New York 1981, in “The Peptides”; Volume 3 (editors: E. Gross and J. Meienhofer), Academic Press, London and New York 1981, in “Methoden der organischen Chemie” (Methods of organic chemistry), Houben Weyl, 4th edition, Volume 15/I, Georg Thieme Verlag, Stuttgart 1974, in H.-D. Jakubke and H. Jescheit, “Aminosäuren, Peptide, Proteine” (Amino acids, peptides, proteins), Verlag Chemie, Weinheim, Deerfield Beach, and Basel 1982, and in Jochen Lehmann, “Chemie der Kohlenhydrate: Monosaccharide und Derivate” (Chemistry of carbohydrates: monosaccharides and derivatives), Georg Thieme Verlag, Stuttgart 1974.
All process steps described here can be carried out under known reaction conditions, preferably under those specifically mentioned, in the absence of or usually in the presence of solvents or diluents, preferably such as are inert to the reagents used and able to dissolve these, in the absence or presence of catalysts, condensing agents or neutralising agents, for example ion exchangers, typically cation exchangers, for example in the H+ form, depending on the type of reaction and/or reactants at reduced, normal, or elevated temperature, for example in the range from −100° C. to about 190° C., preferably from about −80° C. to about 150° C., for example at −80 to −60° C., at room temperature, at −20 to 40° C. or at the boiling point of the solvent used, under atmospheric pressure or in a closed vessel, where appropriate under pressure, and/or in an inert atmosphere, for example under argon or nitrogen.
Salts may be present in all starting compounds and transients, if these contain salt-forming groups. Salts may also be present during the reaction of such compounds, provided the reaction is not thereby disturbed.
The solvents from which those can be selected which are suitable for the reaction in question include for example water, esters, typically lower alkyl-lower alkanoates, e.g diethyl acetate, ethers, typically aliphatic ethers, e.g. diethylether, or cyclic ethers, e.g. tetrahydrofuran, liquid aromatic hydrocarbons, typically benzene or toluene, alcohols, typically methanol, ethanol or 1- or 2-propanol, nitriles, typically acetonitrile, halogenated hydrocarbons, typically dichloromethane, acid amides, typically dimethylformamide, bases, typically heterocyclic nitrogen bases, e.g. pyridine, carboxylic acids, typically lower alkanecarboxylic acids, e.g. acetic acid, carboxylic acid anhydrides, typically lower alkane acid anhydrides, e.g. acetic anhydride, cyclic, linear, or branched hydrocarbons, typically cyclohexane, hexane, or isopentane, or mixtures of these solvents, e.g. aqueous solutions, unless otherwise stated in the description of the process. Such solvent mixtures may also be used in processing, for example through chromatography or distribution.
In a further aspect, the present invention provides novel diamines of formula I,
wherein
Z is —C(O)—, —C(S)— or a bond;
n is 0, 1, 2 or 3;
p is 0 or 1, under the proviso that p is 1 when Z is a bond;
R1 denotes
alkyl which is unsubstituted or mono-, di- or trisubstituted by alkoxy, hydroxy, halogen, amino, alkyl amino, di-alkyl amino, unsubstituted or substituted (C4-C12)aryloxy, unsubstituted or substituted (C5-C12)heterocyclyl containing oxygen, sulfur or nitrogen, unsubstituted or substituted (C3-C8)cycloalkyl or unsubstituted or substituted (C4-C14)aryl; alkenyl, which is unsubstituted or mono-, di- or trisubstituted by alkoxy, hydroxy, halogen, amino, alkyl amino, di-alkyl amino, unsubstituted or substituted (C4-C12)aryloxy, unsubstituted or substituted (C5-C12)heterocyclyl containing oxygen, sulfur or nitrogen, unsubstituted or substituted (C3-C8)cycloalkyl or unsubstituted or substituted (C4-C12)aryl; unsubstituted or substituted (C3-C8)cycloalkyl, unsubstituted or substituted (C5-C12)heterocyclyl containing oxygen, sulfur or nitrogen, unsubstituted or substituted (C4-C12)aryl or unsubstituted or substituted (C4-C12)hetaryl; and
R1′ denotes hydrogen, unsubstituted or substituted (C4-C12)aryl, unsubstituted or substituted (C4-C12)aryl alkyl or
alkyl which is unsubstituted or mono-, di- or trisubstituted by alkoxy, hydroxy, halogen, amino, alkyl amino or di-alkyl amino; or
R1 and R1′ together with the nitrogen atom to which they are attached form an unsubstituted or substituted mono-, di, tri or tetracyclic (C5-C16)heterocyclyl group containing at least one nitrogen atom,
R2 denotes hydrogen or alkyl, unsubstituted or substituted (C4-C12)aryl alkyl or unsubstituted or substituted (C4-C12)aryl,
R3 denotes hydrogen, unsubstituted or substituted (C4-C16)aryl, unsubstituted or substituted (C3-C5)cycloalkyl, (C5-C12)heterocyclyl containing oxygen, sulfur or nitrogen; unsubstituted or substituted (C4-C12)hetaryl; alkyl which is unsubstituted or substituted by hydroxy, amino, (C4-C12)aryl, (C4-C12)hetaryl or unsubstituted or substituted aryl; or R17—C(O)—, wherein R17 denotes alkyl, (C4-C12)aryl or (C4-C12)hetaryl; and
R3′ denotes hydrogen, alkyl, alkyl carbonyl or unsubstituted or substituted (C4-C12)aryl alkyl, or
R3 and R3′ together with the nitrogen atom to which they are attached form an unsubstituted or substituted cyclic (C5-C12)heterocyclyl group containing at least one nitrogen atom, under the proviso that if n is 0, Z is —C(O)—, p is 1, R1 is benzyl, R2 is phenyl and R1′ and R3′ are hydrogen, then R3 does not denote benzyl or acetyl; if n is 0, Z is —C(O)—, p is 1, R1 is phenethyl, R1′ is hydrogen, R2 is phenyl and R3′ is hydrogen, then R3 does not denote hydrogen; if n is 1, Z is a bond, p is 1, R1 is di-phenyl methyl and R1′, R2 and R3′ are all hydrogen, then R3 is not di-phenyl methyl or benzyl; and, if Z is a bond, p is 1, R1 and R1′ together represent the structure of subformula Ic, X represents —CH2—CH2—, R2 is hydrogen and n is 1 or 2, then R3 and R3′ together with the nitrogen atom to which they are attached do not represent piperidinyl and R3 and R3′ are not simultaneously methyl; in free base or acid addition salt form.
According to the present invention, such diamines of formula I are preferred, wherein
Z is —C(O)— or a bond;
n is 0, 1 or 2;
p is 0 or 1, under the proviso that p is 1 when Z is a bond;
R1 denotes
(C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino, di-(C1-6)alkyl amino, aryloxy, 2,3-dihydro-benzo[1,4]dioxanyl, (C4-C7)cycloalkyl or phenyl, which itself is unsubstituted or substituted by halogen, (C1-6)alkoxy or trifluoromethoxy;
(C1-6)alkenyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino, di-(C1-6)alkyl amino, aryloxy, 2,3-dihydro-benzo[1,4]dioxanyl, (C4-C7)cycloalkyl or phenyl, which itself is unsubstituted or substituted by halogen, (C1-6)alkoxy or trifluoromethoxy;
(C4-C7)cycloalkyl, 1,4-dioxan, naphthyl, 9H-fluorenyl, 10,11-dihydro-5H-dibenzo[a,d]cycloheptenyl, 2,3-dihydrobenzo[b]furyl, 2,3-dihydrochromenyl;
phenyl which is unsubstituted or substituted by phenyl or (C1-6)alkoxy; or
piperidinyl which is unsubstituted or substituted by phenyl or pyrimidyl; and
R1′ denotes hydrogen, phenyl, benzyl or
(C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino; or
R1 and R1′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ia, Ib, Ic or Id,
wherein
A denotes N and E denotes CR4R4′ or A denotes CR4R4′ and E denotes N,
G denotes a single bond, CHR19, —CH2CH2—, —CH═CH—, S(O)t or NR20, wherein t is 0, 1 or 2, and R19 and R20, independently of each other, represent hydrogen or (C1-6)alkyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino
X represents oxygen, a single bond, CHR2, —CH═CH— or —CH2—CH2—, S(O)r or NR22, wherein r is 0, 1 or 2, and R21 and R22, independently of each other, represent hydrogen or (C1-6)alkyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
m is 0 or 1,
R4 and R4′ represent, independently of each other, hydrogen or (C1-6)alkyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R5 represents hydrogen, halogen, (C1-6)alkoxy, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di(C1-6)alkyl amino,
R6 represents hydrogen, halogen, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R7 and R8 represent, independently of each other, hydrogen, halogen or (C1-6)alkyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R9 and R10 represent, independently of each other, hydrogen, halogen, (C1-6)alkoxy, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R11 is hydrogen, halogen, (C1-6)alkoxy, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R12 is hydrogen, halogen, (C1-6)alkoxy, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino, and
R13 represents hydrogen or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R2 denotes hydrogen or (C1-6)alkyl, or
benzyl, naphthyl or phenyl, which in each case is unsubstituted or substituted by halogen, (C1-6)alkoxy, (C1-6)alkyl, hydroxy, trifluoromethyl, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
R3 denotes hydrogen, fluorenyl, 2,3-dihydrochromenyl, 1,2,2a,3,4,5-hexahydro-acenaphthyl, 1,2-dihydro-acenaphthyl, tetrahydronaphthyl which is unsubstituted or substituted by (C1-6)alkoxy; benzoxazolyl; cyano-pyridyl; (C1-6)alkyl thieno[2,3-d]-pyrimidyl; N—(C1-6)alkyl piperidinyl, which is substituted by phenyl; (C3-C4)cycloalkyl which is substituted by phenyl; 2,3-dihydro-indenyl; (C1-6)alkyl which is unsubstituted or substituted by hydroxy, amino, naphthyl, indolyl, thiazolyl, pyridyl or phenyl, which itself is unsubstituted or mono- or disubstituted by halogen, phenyl, nitro, di-(C1-6)alkyl amino, di-(C1-6)alkyl amino (C1-6)alkoxy, (C1-6)alkyl, (C1-6)alkoxy, trifluoromethyl or trifluoromethoxy; or R17—C(O)—, wherein R17 denotes (C1-6)alkyl, phenyl, indolyl or benzo[b]furyl, which in each case is substituted by cyano, carboxy or (C1-6)alkoxy carbonyl; and
R3′ denotes hydrogen, (C1-6)alkyl, (C1-6)alkyl carbonyl or benzyl, or
R3 and R3′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ie or If,
wherein Q denotes N and T denotes CR16R16′ or Q denotes CR16R16′ and T denotes N,
R14 denotes hydrogen, halogen, (C1-6)alkoxy, phenyl or (C1-6)alkyl which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di(C1-6)alkyl amino,
R15, R16, R16′ R17 and R18 represent, independently of each other, hydrogen, halogen or (C1-6)alkyl, which is unsubstituted or mono-, di- or trisubstituted by (C1-6)alkoxy, hydroxy, halogen, amino, (C1-6)alkyl amino or di-(C1-6)alkyl amino,
under the proviso that if n is 0, Z is —C(O)—, p is 1, R1 is benzyl, R2 is phenyl and R1′ and R3′ are hydrogen, then R3 does not denote benzyl or acetyl; if n is 0, Z is —C(O)—, p is 1, R1 is phenethyl, R1′ is hydrogen, R2 is phenyl and R3′ is hydrogen, then R3 does not denote hydrogen; if n is 1, Z is a bond, p is 1, R1 is di-phenyl methyl and R1′, R2 and R3′ are all hydrogen, then R3 is not di-phenyl methyl or benzyl; and, if Z is a bond, p is 1, R1 and R1′ together represent the structure of subformula Ic, X represents —CH2—CH2—, R2 is hydrogen and n is 1 or 2, then R3 and R3′ together with the nitrogen atom to which they are attached do not represent piperidinyl and R3 and R3′ are not simultaneously methyl.
More preferred are those diamines of formula I, wherein
Z is —C(O)— or a bond;
n is 0, 1 or 2;
p is 0 or 1, under the proviso that p is 1 when Z is a bond;
R1 denotes
(C1-6)alkyl which is unsubstituted or mono- or disubstituted by phenyl, which itself is unsubstituted or substituted by halogen, (C1-6)alkoxy or trifluoromethoxy, phenoxy, 2,3-dihydro-benzo[1,4]dioxanyl or (C5-C7)cycloalkyl;
(C1-6)alkenyl; naphthyl, 9H-fluorenyl, 10,11-dihydro-5H-dibenzo[a,d]cycloheptenyl, 2,3-dihydrobenzo[b]furyl, 2,3-dihydrochromenyl;
phenyl which is unsubstituted or substituted by phenyl or (C1-6)alkoxy; or
piperidinyl which is substituted by pyrimidyl; and
R1′ denotes phenyl, hydrogen or (C1-6)alkyl; or
R1 and R1′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ia, Ib, Ic or Id,
wherein
A denotes N and E denotes CR4R4′ or A denotes CR4R4′ and E denotes N,
G denotes CH2,
X represents oxygen, a bond, —CH═CH— or —CH2—CH2—,
m is 0 or 1,
R4 and R4′ represent, independently of each other, hydrogen or (C1-6)alkyl,
R5 represents hydrogen, (C1-6)alkyl, CF3 or halogen,
R6 represents hydrogen or (C1-6)alkyl,
R7 and R8 are both hydrogen,
R9 and R10 are both hydrogen,
R11 is hydrogen or (C1-6)alkoxy,
R12 represents hydrogen, and
R13 represents hydrogen or (C1-6)alkyl,
R2 denotes hydrogen, (C1-6)alkyl, benzyl, or phenyl, which is unsubstituted or substituted by halogen,
R3 denotes hydrogen, fluorenyl, 2,3-dihydrochromenyl, 1,2,2a,3,4,5-hexahydro-acenaphthyl, 1,2-dihydro-acenaphthyl, tetrahydronaphthyl which is unsubstituted or substituted by (C1-6)alkoxy; benzoxazolyl; cyano-pyridyl; (C1-6)alkyl thieno[2,3-d]-pyrimidyl; N—(C1-6)alkyl piperidinyl, which is substituted by phenyl; (C3-C4)cycloalkyl which is substituted by phenyl; 2,3-dihydro-indenyl; (C1-6)alkyl which is unsubstituted or substituted by hydroxy, amino, naphthyl, indolyl, thiazolyl, pyridyl or phenyl, which itself is unsubstituted or mono- or disubstituted by halogen, phenyl, nitro, di-(C1-6)alkyl amino, di-(C1-6)alkyl amino (C1-6)alkoxy, (C1-6)alkyl, (C1-6)alkoxy, trifluoromethyl or trifluoromethoxy; or R17—C(O)—, wherein R17 denotes (C1-6)alkyl, phenyl, indolyl or benzo[b]furyl, which in each case is substituted by cyano, carboxy or (C1-6)alkoxy carbonyl; and
R3′ denotes hydrogen, (C1-6)alkyl, (C1-6)alkyl carbonyl or benzyl, or
R3 and R3′ together with the nitrogen atom to which they are attached form a cyclic structure of formula Ie or If,
wherein
Q denotes N and T denotes CR16R16′ or Q denotes CR16R16′ and T denotes N, and R14, R15, R16, R16′ R17 and R18 are all hydrogen,
under the proviso that if n is 0, Z is —C(O)—, p is 1, R1 is benzyl, R2 is phenyl and R1′ and R3′ are hydrogen, then R3 does not denote benzyl or acetyl; if n is 0, Z is —C(O)—, p is 1, R1 is phenethyl, R1′ is hydrogen, R2 is phenyl and R3′ is hydrogen, then R3 does not denote hydrogen; if n is 1, Z is a bond, p is 1, R1 is di-phenyl methyl and R1′, R2 and R3′ are all hydrogen, then R3 is not di-phenyl methyl or benzyl; and, if Z is a bond, p is 1, R1 and R1′ together represent the structure of subformula Ic, X represents —CH2—CH2—, R2 is hydrogen and n is 1 or 2, then R3 and R3′ together with the nitrogen atom to which they are attached do not represent piperidinyl and R3 and R3′ are not simultaneously methyl.
Diamines of formula I and their pharmaceutically acceptable acid addition salts, hereinafter referred to as agents of the invention, exhibit valuable pharmacological properties and are therefore useful as pharmaceuticals.
In particular, the agents of the invention exhibit a marked and selective activatory action at human metabotropic glutamate receptors (mGluRs). This can be determined in vitro for example at recombinant human metabotropic glutamate receptors, especially adenylate cyclase-coupled subtypes thereof such as mGluR7, using different procedures like, for example, measurement of inhibition of forskolin-stimulated cAMP accumulation as described by P. J. Flor et. al., Neuropharmacology Vol. 36, pages 153-159 (1997) and F. Gasparini et al., J. Pharmacol. Exp. Ther. Vol 290, pages 1678-1687 (1999) or by determination to what extent the sub-maximal agonist-induced elevation of GTP-gamma-S binding is enhanced as described by M. Maj et al., Neuropharmacology Vol. 45, 895-906 (2003), and references cited therein. Isolation and expression of human mGluR7 subtypes are described in P. J. Flor et. al., Neuropharmacology Vol. 36, pages 153-159 (1997). Selected agents of the invention show EC50 values for the stimulation of GTP-gamma-S binding, sub-maximally induced by DL-AP4, measured on membranes from recombinant cells expressing human mGluR7b or rat mGluR7a of about 1 nM to about 50 μM. Also, selected agents of the invention show EC50 values for the inhibition of forskolin-stimulated cAMP accumulation on recombinant cells expressing human mGluR7b or rat mGluR7a of about 1 nM to about 50 μM.
The agents of the invention are therefore useful in the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission, and of nervous system disorders regulated full or in part by mGluR7.
Disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission are, for example, epilepsy, cerebral ischemias, especially acute ischemias, eye disorders, especially glaucoma and ischemic diseases of the eye, itch, muscle spasms such as local or general spasticity and, in particular, convulsions or pain.
Nervous system disorders regulated full or in part by mGluR7 are for example acute, traumatic and chronic degenerative processes of the nervous system, such as Parkinson's disease, dementia associated with Parkinson's Disease, senile dementia, Alzheimer's disease, Huntington's chorea, amyotrophic lateral sclerosis and multiple sclerosis, psychiatric diseases such as schizophrenia, anxiety disorders and depression.
The usefulness of the agents of the invention in the treatment of the above-mentioned disorders can be confirmed in a range of standard tests including, as an example, the one indicated below:
Activity of the agents of the invention in anxiety can be demonstrated in standard models such as the stress-induced hyperthermia in mice [cf. A. Lecci et al., Psychopharmacol. Vol. 101, pages 255-261 (1990)]. At doses of about 0.1 to about 100 mg/kg p.o. or i.p., the agents of the invention reverse the stress-induced hyperthermia.
For all the above mentioned indications, the appropriate dosage will of course vary depending upon, for example, the compound employed, the host, the mode of administration and the nature and severity of the condition being treated. However, in general, satisfactory results in animals are indicated to be obtained at a daily dosage of from about 0.5 to about 100 mg/kg animal body weight. In larger mammals, for example humans, an indicated daily dosage is in the range from about 5 to 1500 mg, preferably about 10 to about 1000 mg of the compound conveniently administered in divided doses up to 4 times a day.
In accordance with the foregoing, the present invention also provides an agent of the invention for use as a pharmaceutical, e.g. in the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission, and of nervous system disorders regulated full or in part by mGluR7.
The invention also provides the use of an agent of the invention, in the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission, and of nervous system disorders regulated full or in part by mGluR7.
Furthermore the invention provides the use of an agent of the invention for the manufacture of a pharmaceutical composition designed for the treatment of disorders associated with irregularities of the glutamatergic, GABAergic and/or monoaminergic signal transmission, and of nervous system disorders regulated full or in part by mGluR7.
In a further aspect the invention relates to a method of treating disorders regulated full or in part by mGluR7, which method comprises administering to a warm-blooded organism in need of such treatment a therapeutically effective amount of an agent of the invention.
The pharmaceutical compositions according to the invention are compositions for enteral, such as nasal, rectal or oral, or parenteral, such as intramuscular or intravenous, administration to warm-blooded animals (human beings and animals) that comprise an effective dose of the pharmacological active ingredient alone or together with a significant amount of a pharmaceutically acceptable carrier. The dose of the active ingredient depends on the species of warm-blooded animal, body weight, age and individual condition, individual pharmacokinetic data, the disease to be treated and the mode of administration.
The pharmaceutical compositions comprise from approximately 1% to approximately 95%, preferably from approximately 20% to approximately 90%, active ingredient. Pharmaceutical compositions according to the invention may be, for example, in unit dose form, such as in the form of ampoules, vials, suppositories, dragées, tablets or capsules.
The pharmaceutical compositions of the present invention are prepared in a manner known per se, for example by means of conventional dissolving, lyophilizing, mixing, granulating or confectioning processes.
A compound of formula I can be administered alone or in combination with one or more other therapeutic agents, possible combination therapy taking the form of fixed combinations or the administration of a compound of the invention and one or more other therapeutic agents being staggered or given independently of one another, or the combined administration of fixed combinations and one or more other therapeutic agents.
For instance, if the disorder to be treated is epilepsy, the other therapeutic agents can be selected from barbiturates and derivatives thereof, benzodiazepines, carboxamides, hydantoins, succinimides, valproic acid and other fatty acid derivates, AMPA antagonists and other anti-epileptic drugs. The term “barbiturates and derivatives thereof” as used herein includes, but is not limited to Phenobarbital and primidon. The term “benzodiazepines” as used herein includes, but is not limited to clonazepam, diazepam and lorazepam. The term “carboxamides” as used herein includes, but is not limited to carbamazepine and oxcarbazepine. The term “hydantoins” as used herein includes, but is not limited to phenyloin. The term “succinimides” as used herein includes, but is not limited to ethosuximide, phensuximide and mesuximide. The term “valproic acid and other fatty acid derivates” as used herein includes, but is not limited to valproic acid sodium salt, tiagabine hydrochloride monohydrate and vigrabatrine. The term “other anti-epileptic drugs” as used herein includes, but is not limited to levetiracetam, lamotrigine, gabapentin, sultiam and felbamate.
If the disorder to be treated is Alzheimer's disease, the other therapeutic agents is preferably a nootropic. The term “nootropic” as used herein includes, but is not limited to calcium antagonists, cholinesterase inhibitors, dihydroergotoxin, nicergoline, piracetame, purine derivates, pyritinol, vincamine and vinpocetine. The term “calcium antagonists” as used herein includes, but is not limited to cinnarizine and nimodipine. The term “cholinesterase inhibitors” as used herein includes, but is not limited to donepezil hydrochloride, rivastigmine and galantamine hydrobromide. The term “purine derivates” as used herein includes, but is not limited to pentifyllin.
If the disorder to be treated is schizophrenia, the other therapeutic agents can be selected from conventional antipsychotics and atypical antipsychotics. The term “conventional antipsychotics” as used herein includes, but is not limited to haloperidol, fluphenazine, thiotixene and flupentixol. The term “atypical antipsychotics” as used herein relates to clozaril, risperidone, olanzapine, quetiapine, ziprasidone and aripiprazol. If the disorders to be treated are anxiety disorders, the other therapeutic agents can be selected from the group consisting of benzodiazepines, selective serotonin reuptake inhibitors (SSRIs), selective serotonin and norepinephrine reuptake inhibitors (SNRIs), buspirone and pregabalin. The term “benzodiazepines” as used herein includes, but is not limited to clonazepam, diazepam and lorazepam. An SSRI suitable for the present invention is especially selected from fluoxetine, fuvoxamine, sertraline, paroxetine, citalopram and escitalopram. An SNRI suitable for the present invention is especially selected from venlafaxine and duloxetine.
The structure of the active ingredients identified by code nos., generic or trade names may be taken from the actual edition of the standard compendium “The Merck Index” or from databases, e.g. Patents International (e.g. IMS World Publications). The corresponding content thereof is hereby incorporated by reference. Any person skilled in the art is fully enabled to identify the active ingredients and, based on these references, likewise enabled to manufacture and test the pharmaceutical indications and properties in standard test models, both in vitro and in vivo.
In further aspects, the present invention provides
Further, properly isotope-labeled agents of the invention exhibit valuable properties as histopathological labeling agents, imaging agents and/or biomarkers, hereinafter “markers”, for the selective labeling of the metabotropic glutamate receptor subtype 7 (mGlu7 receptor). More particularly the agents of the invention are useful as markers for labeling the central and peripheral mGlu7 receptors in vitro or in vivo. In particular, compounds of the invention which are properly isotopically labeled are useful as PET markers. Such PET markers are labeled with one or more atoms selected from the group consisting of 11C, 13N, 15O, 18F.
The agents of the invention are therefore useful, for instance, for determining the levels of receptor occupancy of a drug acting at the mGlu7 receptor, or diagnostic purposes for diseases resulting from an imbalance or dysfunction of mGlu7 receptors, and for monitoring the effectiveness of pharmacotherapies of such diseases.
In accordance with the above, the present invention provides an agent of the invention for use as a marker for neuroimaging.
In a further aspect, the present invention provides a composition for labeling brain and peripheral nervous system structures involving mGlu7 receptors in vivo and in vitro comprising an agent of the invention.
In still a further aspect, the present invention provides a method for labeling brain and peripheral nervous system structures involving mGlu7 receptors in vitro or in vivo, which comprises contacting brain tissue with an agent of the invention.
The method of the invention may comprise a further step aimed at determining whether the agent of the invention labeled the target structure. Said further step may be effected by observing the target structure using positron emission tomography (PET) or single photon emission computed tomography (SPECT), or any device allowing detection of radioactive radiations.
The following Examples illustrate the invention.
Abbreviations used are those conventional in the art and, in particular, have the meanings provided below.
BAL=Backbone amide linker
EE=Ethyl acetate
ESI-MS=Electro-spray ionization mass spectroscopy
HATU=O-(7-Azobenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate
HPLC=High performance liquid chromatography
m.p.=Melting point
MTBE=Methyl tert.-butyl ether
NMR=Nuclear magnetic resonance spectroscopy
Rf=Retention factor (TLC)
RT=Room temperature
TFA=Trifluoroacetic acid
TLC=Thin layer chromatography
tR=Retention time (HPLC)
Temperatures are measured in degrees Celsius. Unless indicated otherwise, reactions are carried out at room temperature. The structure of final products, intermediates and starting materials is confirmed by standard analytical methods, e.g. microanalysis and spectroscopic characteristics (e.g. MS, IR, NMR).
Gradient A: Performed on a Waters system equipped with a CTC Analytics HTS PAL autosampler, 515 pumps, and a 996 DAD detector operating at 210 nm. Column: CC70/3 Nucleosil 100-3 C18 (3μ, 70×3 mm, Macherey-Nagel, order # 721791.30), temperature: 45° C., flow: 0.8 mL min−1. Eluents: A: Water+0.2% H3PO4 (85%, (Merck 100552)+2% Me4NOH, (10%, Merck 108123), B: Acetonitril+20% water+0.1% H3PO4 (85%)+1% Me4NOH (10%). Gradient: 0% B to 95% B within 10 min., then 95% B 5 min.
Gradient B: Performed on a Waters 2690 system equipped with a 996 DAD detector operating at 220-400 nm (Total Plot). Column: CC30/3 Nucleosil 100-3 C18 HD (3μ, 30×3 mm, Macherey-Nagel, order # 721196.30), temperature: 35° C., flow: 1 mL min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 5% B to 100% B within 4 min., then 100% B 1 min.
Gradient C: Performed on a Agilent 1100 series system equipped with DAD detector operating at 254 nm. Column: ZORBAX Eclips XDB C18 (5μ, 150×4.6 mm, Agilent, order # 7995118-595), flow: 1.5 mL min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 0% B to 100% B within 15 min., then 100% B 5 min.
Gradient D: Performed on a Agilent 1100 series device and UV detection at 215 nm. Column: Prontosil RP-C18-H (3μ, 53×4 mm, Bischoff, order # 0604F185PS030), flow: 1.5 mL min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 5% B to 100% B within 10 min.
Gradient E: Performed on a Agilent 1100 series device and UV detection at 215 nm. Column: Nucleodur RP-C18-ec (3μ, 53×4 mm, Macherey-Nagel, order # 760050.40), flow: 1 mL min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 5% B to 100% B within 10 min.
Gradient F: Performed on a Waters system equipped with 600 pump, ZQ Micromass MS-detector, and a UV 2487 detector operating at 214 nm. Column: XTerra Prep MS C18 (5μ 50×19 mm, Waters, order # 18600 1930), flow: 20 ml min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 5% B for 1 min. then 5% B to 95% B within 6 min.
Gradient G: Performed on a Shimadzu system equipped with SCL-8A controller, 2×LC-8A pumps, and a Bischoff Lambda 1000 detector operating at 215 nm. Column: Waters Symmetry C18 (5μ 50×19 mm, Waters, order # 18600 0210), flow: 20 ml min−1. Eluents: A: Water+0.1% TFA, B: Acetonitril+0.1% TFA. Gradient: 5% B to 100% B within 15 min.
A solution of chloro-acetylchloride (4.48 ml, 56.3 mmol) in toluene (5 ml) is added at room temperature to a stirred solution of diphenylamine (8.66 g 51.1 mmol) and triethylamine (8.6 ml, 61.4 mmol) in toluene (45 ml). The resulting solution is stirred for 90 min. at room temperature and is then brought to reflux for 18 hours. The toluene is evaporated and the residue is dissolved in DCM, washed consecutively with a diluted HCl solution (2N) and brine. The organic layer is dried with sodium sulfate and evaporated. The residue is purified by chromatography (CH2Cl2/hexanes, 4:1) to afford 2-chloro-N,N-diphenyl-acetamide as tan crystals (modified method according to R. Sarges et al., J. Med. Chem. 1989 32(2), 437-444).
b) A solution of 2-chloro-N,N-diphenyl-acetamide (5 g, 20.3 mmol), 3,4-dichlorobenzyl-amine (8.95 g, 50.9 mmol) in ethanol (30 ml) is refluxed for 12 hours. The solution is concentrated to about 10 ml and the suspension is filtered off. The filtrate is evaporated and the residue is filtered over silica (CH2Cl2/MeOH, 98:2). The purified fraction is dissolved in a saturated solution of HCl in ethyl acetate from which the hydrochloric salt was filtered off after cooling. The title compound is obtained as colorless crystals: 1H-NMR (400 MHz; DMSO-d6): δ 3.75 (s, 2H); 4.15 (s, 2H); 7.2-7.6 (m, 11H); 7.7 (d, 1H); 7.8 (d, 1H); 9.55 (NH 1H), ESI+ MS: m/z=385.1 (MH+).
a) To an ice cooled solution of diphenyl-amine (4.87 g, 28.8 mmol) and pyridine (2.52 ml, 31.4 mmol) in 1,2-dichloroethane (40 ml) is added a solution of bromoacetylbromide (2.5 ml, 30.0 mmol) in 1,2-dichloroethane (10 ml) at such a rate that the internal temperature of the reaction vessel remains below 30° C. After completion of the addition the ice bath is removed and the reaction mixture is stirred for 1 h at room temperature. Then, the reaction mixture is poured on ice water (100 ml). The mixture is made acidic by addition of 2 M HCl and ethyl acetate (50 ml) is added. The organic layer is separated, washed (2× water (100 ml), 1×brine (100 ml)), dried over Na2SO4, filtered and evaporated in vacuo. The obtained brown oil is purified by recrystallization from diethyl ether to provide 2-bromo-N,N-diphenyl-acetamide as off white crystals. (modified method according to F. Oezkanli et al., Arzneim. Forsch. 1994 44(8), 920-924).
b) A solution of 2-bromo-N,N-diphenyl-acetamide (200 mg, 0.69 mmol) and 2-chlorobenzylamine (208 μl, 1.73 mmol) in dry ethanol (1.5 ml) is heated to 80° C. with stirring in a 4 ml Pierce Reacti-Vial over night. After reaching room temperature the reaction mixture is poured on a mixture of ethyl acetate (10 ml) and saturated NaHCO3 solution (10 ml). The organic layer is separated, washed (1×H2O, 2×brine), dried over Na2SO4, and evaporated to dryness. The residue is purified by flash chromatography (FlashMaster chromatography system, 10 g Flash-Si cartridge (IST ISOLUTE® SPE, ethyl acetate/hexanes gradient) to give a yellow oil. The free base is dissolved in ethyl acetate (2 ml) and acidified by addition of a few drops of 4M HCl in dioxane. The precipitate is filtered off and washed with diethyl ether. Drying in vacuo at 60° C. over night give 2-(2-Chloro-benzylamino)-N,N-diphenyl-acetamide hydrochloride as pale yellow powder. M.p.=213-214.5° C.; HPLC: tR=6.99 min (gradient A), ESI+ MS: m/z=351 (MH+).
Following the same procedures as described under Examples 1 and 2 the following compounds are obtained using suitable starting materials:
M.p.=232-234° C.; HPLC: tR=8.18 min (gradient A); ESI+ MS: m/z=407.5 (MH+).
M.p.=233-235° C.; HPLC: tR=7.60 min (gradient A); ESI+ MS: m/z=367.5 (MH+).
M.p.=98-99° C.; HPLC: tR=7.56 min (gradient A); ESI+ MS: m/z=365.3 (MH+).
M.p.=182-186° C.; HPLC: tR=6.58 min (gradient A); ESI+ MS: m/z=315.2 (MH+).
M.p.=174-178° C.; HPLC: tR=8.07 min (gradient A); ESI+ MS: m/z=371.5 (MH+).
M.p.=221-223° C.; HPLC: tR=6.11 min (gradient A); ESI+ MS: m/z=345.5 (MH+).
M.p.=221-224° C.; HPLC: tR=6.04 min (gradient A); ESI+ MS: m/z=323.2 (MH+).
M.p.=72-78° C.; HPLC: tR=7.93 min (gradient A); ESI+ MS: m/z=371.5 (MH+).
M.p.=240-243° C.; HPLC: tR=8.19 min (gradient A); ESI+ MS: m/z=373.5 (MH+).
M.p.=224-226° C.; HPLC: tR=7.50 min (gradient A); ESI+ MS: m/z=333.3 (MH+).
M.p.=245-250° C.; HPLC: tR=7.61 min (gradient A); ESI+ MS: m/z=351.3 (MH+).
M.p.=202-204° C.; HPLC: tR=7.11 min (gradient A); ESI+ MS: m/z=343.4 (MH+).
M.p.=122-123° C.; HPLC: tR=8.56 min (gradient A); ESI+ MS: m/z=393.5 (MH+).
M.p.=215-216° C.; HPLC: tR=8.77 min (gradient A); ESI+ MS: m/z=421.5 (MH+).
M.p.=250° C.; HPLC: tR=9.06 min (gradient A); ESI+ MS: m/z=405.3 (MH+).
M.p.=132-134° C.; HPLC: tR=8.85 min (gradient A); ESI+ MS: m/z=367.3 (MH+).
M.p.=151-158° C.; HPLC: tR=10.74 min (gradient A); ESI+ MS: m/z=393.4 (MH+).
M.p.=188-190° C.; HPLC: tR=8.83 min (gradient A); ESI+ MS: m/z=421.6 (MH+).
M.p.=190-192° C.; HPLC: tR=7.63 min (gradient A); ESI+ MS: m/z=365.4 (MH+).
M.p.=181-182° C.; HPLC: tR=8.97 min (gradient A); ESI+ MS: m/z=407.6 (MH+).
M.p.=191-194° C.; HPLC: tR=7.90 min (gradient A), ESI+ MS: m/z=357.6 (MH+).
M.p.=115-116° C.; HPLC: tR=7.24 min (gradient A); ESI+ MS: m/z=331.5 (MH+).
M.p.=241-243° C.; HPLC: tR=8.09 min (gradient A); ESI+ MS: m/z=359.6 (MH+).
M.p.=82-95° C.; HPLC: tR=8.25 min (gradient A); ESI+ MS: m/z=385.3 (MH+).
M.p.=128-136° C.; HPLC: tR=8.22 min (gradient A), ESI+ MS: m/z=371.3 (MH+).
M.p.=212-215° C.; HPLC: tR=8.21 min (gradient A), ESI+ MS: m/z=385.5 (MH+).
Yellow gum, HPLC: tR=7.23 min (gradient A), ESI+ MS: m/z=323.4 (MH+).
Yellow gum, HPLC: tR=6.65 min (gradient A), ESI+ MS: m/z=309.5 (MH+).
M.p.=82-84° C.; HPLC: tR=7.75 min (gradient A), ESI+ MS: m/z=355.6 (MH+).
M.p.=210-212° C.; HPLC: tR=8.20 min (gradient A), ESI+ MS: m/z=385.8 (MH+).
M.p.=201-203° C.; HPLC: tR=6.63 min (gradient A), ESI+ MS: m/z=307.3 (MH+).
Pale brown syrup, HPLC: tR=10.57 min (gradient A), ESI+ MS: m/z=321.4 (MH+).
M.p.=232-236° C.; 1H-NMR (400 MHz; CDCl3): δ 1.36 (d, J=8 Hz, 3H), 3.13 (s, 2H), 3.77 (q, J=8 Hz, 1H), 7.08-7.35 (m, 15H); ESI+ MS: m/z=331.2 (MH+).
M.p.=245-246° C.; HPLC: tR=2.65 min (gradient B), ESI+ MS: m/z=381.2 (MH+).
M.p.=234-237° C.; HPLC: tR=2.40 min (gradient B), ESI+ MS: m/z=317.2 (MH+).
HPLC: tR=2.64 min (gradient B), ESI+ MS: m/z=357.2 (MH+).
M.p.=167-271° C.; HPLC: tR=2.73 min (gradient B), ESI+ MS: m/z=411.2 (MH+).
M.p.=227-230° C.; HPLC: tR=2.94 min (gradient B), ESI+ MS: m/z=421.2 (MH+).
M.p.=250-253° C.; HPLC: tR=2.48 min (gradient B), ESI+ MS: m/z=331.2 (MH+).
M.p.=244-249° C.; HPLC: tR=2.82 min (gradient B), ESI+ MS: m/z=409.0 (MH+).
M.p.=243-259° C.; HPLC: tR=2.58 min (gradient B), ESI+ MS: m/z=355.2 (MH+).
a) 2-Bromo-1-(3,4-dihydro-2H-quinolin-1-yl)-ethanone is prepared analogously to bromo-N,N-diphenyl-acetamide (Example 1, step a) from 1,2,3,4-tetrahydroquinoline (7.89 ml, 59.1 mmol), pyridine (5.23 ml, 65.0 mmol) and bromoacetylbromide (5.77 ml, 65 mmol) in 1,2-dichloroethane (60 ml). The title compound is obtained as greenish oil.
b) To a solution of 2-bromo-1-(3,4-dihydro-2H-quinolin-1-yl)-ethanone (25 mg, 0.1 mmol) and rac-1-phenylethylamine (25 μl, 0.2 mmol) in N,N-dimethyl acetamide (0.6 ml) is added a 30% Na2CO3 solution in water (75 μl, 0.21 mmol). The reaction mixture is shaken in a 4.5 ml fritted polypropylene reaction tube for 24 h at 50° C. (Mettler-Toledo Bohdan 48 position MiniBlock™ synthesizer). Then, the solution is filtered and directly submitted to preparative HPLC (gradient F). The title compound is obtained as a yellow gum; HPLC: tR=6.64 min (gradient A); ESI+ MS: m/z=295 (MH+).
Following the same procedures as described under Example 44 the following compounds are obtained using suitable starting materials:
HPLC: tR=7.40 min (gradient A), ESI+ MS: m/z=345.5 (MH+).
HPLC: tR=7.24 min (gradient A); ESI+ MS: m/z=365.3 (MH+).
HPLC: tR=6.59 min (gradient A), ESI+ MS: m/z=309.4 (MH+).
HPLC: tR=7.06 min (gradient A), ESI+ MS: m/z=329.3 (MH+).
HPLC: tR=6.54 min (gradient A), ESI+ MS: m/z=323.4 (MH+).
HPLC: tR=7.69 min (gradient A), ESI+ MS: m/z=401.6 (MH+).
HPLC: tR=7.34 min (gradient A), ESI+ MS: m/z=370.6 (MH+).
HPLC: tR=6.65 min (gradient A), ESI+ MS: m/z=347.4 (MH+).
HPLC: tR=7.54 min (gradient A), ESI+ MS: m/z=365.4 (MH+).
HPLC: tR=7.70 min (gradient A), ESI+ MS: m/z=383.6 (MH+).
HPLC: tR=7.50 min (gradient A), ESI+ MS: m/z=345.5 (MH+).
HPLC: tR=7.48 min (gradient A), ESI+ MS: m/z=385.5 (MH+).
HPLC: tR=7.74 min (gradient A), ESI+ MS: m/z=400.7 (MH+).
HPLC: tR=7.46 min-(gradient A), ESI+ MS: m/z=343.4 (MH+).
HPLC: tR=7.45 min (gradient A), ESI+ MS: m/z=387.7 (MH+).
HPLC: tR=8.33 min (gradient A), ESI+ MS: m/z=407.7 (MH+).
HPLC: tR=7.82 min (gradient A), ESI+ MS: m/z=381.7 (MH+).
HPLC: tR=7.48 min (gradient A), ESI+ MS: m/z=365.5 (MH+).
HPLC: tR=7.38 min (gradient A), ESI+ MS: m/z=343.4 (MH+).
HPLC: tR=7.37 min (gradient A), ESI+ MS: m/z=395.3 (MH+).
HPLC: tR=7.33 min (gradient A), ESI+ MS: m/z=331.4 (MH+).
HPLC: tR=7.69 min (gradient A), ESI+ MS: m/z=385.4 (MH+).
HPLC: tR=7.61 min (gradient A), ESI+ MS: m/z=361.4 (MH+).
HPLC: tR=8.21 min (gradient A), ESI+ MS: m/z=393.5 (MH+).
HPLC: tR=7.90 min (gradient A), ESI+ MS: m/z=379.4 (MH+).
HPLC: tR=6.51 min (gradient A), ESI+ MS: m/z=295.4 (MH+).
HPLC: tR=6.54 min (gradient A), ESI+ MS: m/z=295.4 (MH+).
HPLC: tR=8.83 min (gradient A), ESI+ MS: m/z=489.7 (MH+).
HPLC: tR=7.93 min (gradient A), ESI+ MS: m/z=411.6 (MH+).
HPLC: tR=5.23 min (gradient A), ESI+ MS: m/z=371.2 (MH+).
HPLC: tR=5.51 min (gradient A), ESI+ MS: m/z=385.6 (MH+).
HPLC: tR=5.11 min (gradient A), ESI+ MS: m/z=364.3 (MH+).
HPLC: tR=5.16 min (gradient A), ESI+ MS: m/z=359.1 (MH+).
HPLC: tR=4.63 min (gradient A), ESI+ MS: m/z=337.0 (MH+).
HPLC: tR=4.97 min (gradient A), ESI+ MS: m/z=391.0 (MH+).
HPLC: tR=5.26 min (gradient A), ESI+ MS: m/z=389.1 (MH+).
HPLC: tR=6.02 min (gradient A), ESI+ MS: m/z=413.1 (MH+).
HPLC: tR=4.51 min (gradient A), ESI+ MS: m/z=309.0 (MH+).
a) To a solution of α-aminodiphenylmethane (3.40 ml, 20 mmol) in chloroform (20 ml) is added triethylamine (1.4 ml, 10 mmol), ethyl bromoacetate (1.1 ml, 10 mmol), and molecular sieves 4 Å. The reaction mixture is shaken at room temperature under argon for 20 h (Bücchi Syncore apparatus). Then, the molecular sieves is filtered off and the filtrate extracted once with water. The aqueous layer is washed with chloroform once. The combined organic layers are dried (Na2SO4) and evaporated to give a yellow syrup. The crude product is purified by flash chromatography to afford ethyl (benzhydryl-amino)-acetate as a colorless oil.
b) A solution of ethyl (benzhydryl-amino)-acetate (781 mg, 2.9 mmol) in 2M KOH in methanol (5 ml, 10 mmol) is refluxed for 2 h. Then, the solvent is evaporated and the residue re-dissolved in water. The pH of the solution is adjusted to 7.3 by addition of 2M HCl. The precipitated crystals are collected, washed with water and high vacuum dried to give (benzhydryl-amino)-acetic acid as colorless crystals.
c) To a solution of (benzhydryl-amino)-acetic acid (139 mg, 0.5 mmol) in DMF (5 ml) is added triethylamine (209 μl, 1.5 mmol), HOBt (122 mg, 0.9 mmol), DIC (116 μl, 0.75 mmol), and 3,3-diphenylpropylamine (106 mg, 0.5 mmol). The reaction mixture is shaken on a Bücchi Syncore reactor for 72 h at room temperature. The mixture is diluted with water and extracted twice with toluene. The combined organic layers are dried (Na2SO4) and evaporated under reduced pressure. The residue is dissolved in ether and filtered over Hyflo. To the clear filtrate 4 M HCl in dioxane (125 μl, 0.5 mmol) is added and the suspension obtained is stirred for 15 min. The white precipitate is filtered off, washed with ether and high vacuum dried to afford the title compound as colorless crystals: M.p.=177.5-178.1° C.; HPLC: tR=11.52 min (gradient C), ESI+ MS: m/z=435.2 (MH+).
The title compound is prepared by coupling of (benzhydryl-amino)-acetic acid (example 44, step b) with benzylamine according to the procedure given in example 44, step c) and is obtained as colorless crystals: M.p.=161.9-162.9° C.; HPLC: tR=12.08 min (gradient C), ESI+ MS: m/z=330.9 (MH+).
a) 2-Amino-N,N-diphenyl-acetamide: A solution of 2-chloro-N,N-diphenyl-acetamide (8.5 g, 34.6 mmol, Example 1, step a) in methanol saturated with ammonia (800 ml, 2.8 M) is stirred at 60° C. for 19 hours. Then another 80 ml of the ammonia in methanol is added and stirring is continued for 24 hours at 60° C. Evaporation of the solvent and chromatography on silica gel (DCM/methanol gradient) yields pure fractions of a yellow oil which, upon standing solidifies. M.p.=108-110° C.
b) 2-[2-(2-Hydroxy-ethoxy)-benzylamino]-N,N-diphenyl-acetamide hydrochloride: To a solution of 2-amino-N,N-diphenyl-acetamide (102 mg, 0.451 mmol) in methanol (1 ml) is added 2-(2-hydroxy-ethoxy)-benzaldehyde (50 mg, 0.301 mmol), acetic acid (26 μl, 0.451 mmol) and sodium triacetoxyborohydride (191 mg, 0.903 mmol). After stirring the mixture for 1.5 hours at room temperature the solvent is removed in vacuo and the residue is dissolved in DCM, washed consecutively twice with an aqueous sodium hydroxide solution (1M) and brine. The organic layer is dried with sodium sulfate and evaporated. The residue is purified by chromatography on silica gel (DCM/methanol gradient) and the obtained title compound converted into its hydrochloride salt by treatment of a dioxan solution with hydrogen chloride in dioxan. M.p.=90-160° C.; HPLC: tR=2.34 min (gradient B), ESI+ MS: m/z=377.2 (MH+).
The title compound is obtained according to the procedure described in Example 85. m.p.=274-295° C.; HPLC: tR=2.60 min (gradient B), ESI+ MS: m/z=357.2 (MH+).
a) (R)-({[(Diphenylcarbamoyl)-methyl]-carbamoyl}-phenyl-methyl)-carbamic acid tert-butyl ester: To a suspension of (R)-tert-butoxycarbonylamino-phenyl-acetic acid (100 mg, 0.398 mmol) and 2-amino-N,N-diphenyl-acetamide (90 mg, 0.398 mmol, Example 85, step a) in DCM/THF (1.5 ml, 2:1) is added pyridine (6 μl, 0.08 mmol), DCC (90 mg, 0.438 mmol) and HOBT (54 mg, 0.398 mmol) and the mixture is stirred at room temperature for 16 hours. The reaction suspension is filtered, the filtrate diluted with ethyl acetate and washed consecutively three times with a saturated aqueous solution of sodium hydrogen carbonate. The organic layer is dried with sodium sulfate and evaporated to yield the desired product as a yellow oil which is used in the next step without further purification. HPLC: tR=3.14 min (gradient B), ESI+ MS: m/z=482.2 (M-Na+).
b) (R)-2-Amino-N-[(diphenylcarbamoyl)-methyl]-2-phenyl-acetamide: ({[(Diphenylcarbamoyl)-methyl]-carbamoyl}-phenyl-methyl)-carbamic acid tert-butyl ester (95 mg, 0.207 mol) is treated with 1 ml of a solution of hydrogen chloride in dioxan (4 M) for 10 minutes. The light brown oil obtained after evaporation of the solvent is crystallized from DCM and diethyl ether to afford the title compound as a white powder. M.p.=177-185° C. (dec.); 1H-NMR (400 MHz; CDCl3): δ 3.94 (d, J=7 Hz, 2H), 4.53 (s, 1H), 7.10-7.45 (m, 15H), 7.82 (b, 1H); ESI+ MS: m/z=360.2 (MH+).
a) 2-Amino-1-dibenzo[b,f]azepin-5-yl-ethanone: This compound is prepared in a similar way as in Example 85, step a). HPLC: tR=2.07 (gradient B), ESI+ m/z=251.2 (MH+).
b) (R)-[(2-Dibenzo[b,f]azepin-5-yl-2-oxo-ethylcarbamoyl)-phenyl-methyl]-carbamic acid tert-butyl ester: This compound is prepared in a similar way as in Example 87 step a). HPLC: tR=3.14 (gradient c), ESI+ m/z=506.2 (M-Na+).
c) (R)-2-Amino-N-(2-dibenzo[b,f]azepin-5-yl-2-oxo-ethyl)-2-phenyl-acetamide hydrochloride: This compound is prepared in a similar way as in example 87 step b). M.p. 179-185° C.; HPLC: tR=2.43 (gradient B), ESI+ m/z=384.0 (MH+).
This compound is prepared in accordance with the procedures as described in Example 88. M.p. 192-233° C.; HPLC: tR=2.43 (gradient B), ESI+ m/z=384.2 (MH+).
The following examples are prepared according to the procedure outlined in Example 2.
M.p.=122-125° C.; HPLC: tR=8.79 min (gradient A), ESI+ MS: m/z=421.6 (MH+).
M.p.=208-210° C.; HPLC: tR=7.61 min (gradient A), ESI+ MS: m/z=357.6 (MH+).
The following examples are prepared according to the procedure outlined in Example 44.
HPLC: tR=7.47 min (gradient A), ESI+ MS: m/z=345.5 (MH+).
HPLC: tR=8.08 min (gradient A), ESI+ MS: m/z=399.4 (MH+).
HPLC: tR=8.40 min (gradient A), ESI+ MS: m/z=407.6 (MH+).
The title compound is prepared according to the procedure outlined in Example 83. Colorless crystals: m.p.=210.3-212.0° C.; HPLC: tR=15.92 min (gradient C), ESI+ MS: m/z=448.9 (MH+).
The following examples are prepared according to the procedure outlined in Example 44.
M.p.=192-198° C.; HPLC: tR=7.91 min (gradient A), ESI+ MS: m/z=382.1 (MH+).
M.p.=239-241° C.; HPLC: tR=7.50 min (gradient A), ESI+ MS: m/z=358.2 (MH+).
a) tert.-Butyl benzylcarbamoyl-phenyl-methyl)-carbamate: A solution of tert.-butoxycarbonyl-amino-phenyl-acetic acid (7.54 g, 30 mmol), 4-N,N-dimethylamino-pyridine (1.83 g, 15 mmol) in THF (150 ml) is stirred for 5 minutes at room temperature. Di-(N-succinimidyl)carbonate (7.69 g, 30 mmol) is added and the resulting mixture is stirred for 18 hours. Benzylamine (3.2 g, 30 mmol) is added and the solution is stirred for another 18 hours at room temperature. The resulting suspension is filtered and the filtrate is evaporated. The residue is dissolved in dichloromethane and washed successively with a NaHSO4 solution (2N), a saturated K2CO3 solution and brine. The organic layer is dried over MgSO4 and evaporated. The crude product is recrystallized from ether to give the desired compound.
b) 2-Amino-N-benzyl-2-phenyl-acetamide: (Benzylcarbamoyl-phenyl-methyl)-carbamic acid tert-butyl ester (8.7 g, 25.4 mmol) is dissolved in HCl saturated ethyl-acetate (100 ml). The solution is stirred at room temperature over night. The solution is concentrated and the resulting crystals are dissolved in CH2Cl2 and washed with a saturated solution of K2CO3. The organic layer is dried and evaporated to afford the desired compound.
c) N-Benzyl-2-benzylamino-2-phenyl-acetamide fumarate: 2-Amino-N-benzyl-2-phenylacet-amide (529 mg, 2.2 mmol), benzaldehyde (202 μl, 2 mmol) are stirred for 2 hours at room temperature in a solution of 1,2-dichloroethane, methanol and acetic acid (6:3:1, 20 ml). Polymer supported cyano-borohydride (1 g, 4.3 mmol) is added and the mixture is stirred for 18 hours. The suspension is filtered, the filtrate is evaporated and the residue is purified by chromatography (CH2Cl2/MeOH, 99:1) to afford the desired compound. The compound is dissolved in ethanol and fumaric acid (1 mole-equivalent) is added. The solution is brought to its boiling point and cooled to afford the fumarate salt. ESI+ MS: m/z=331.2 (MH+).
The following compounds are prepared in accordance to the procedures as described in Example 99 starting with the appropriate amines and aldehydes:
ESI+ MS: m/z=408.2 (MH+).
ESI+ MS: m/z=410.1 (MH+).
Colorless crystals, ESI+ MS: m/z=422.2 (MH+).
Colorless crystals, mp. 153-155° C., ESI+ MS: m/z=361.3 (MH+).
Colorless crystals, ESI+ MS: m/z=433.2 (MH+).
Colorless crystals, ESI+ MS: m/z=395.2 (MH+).
Colorless crystals, mp. 109-119.5° C., ESI+ MS: m/z=421.2 (MH+).
Yellow crystals, mp. 159-161° C., ESI+ MS: m/z=374.3 (MH+).
Colorless crystals, mp. 142-205° C. (dec.), ESI+ MS: m/z=365.2 (MH+).
Colorless crystals, ESI+ MS: m/z=433.1 (MH+).
Colorless crystals, ESI+ MS: m/z=331.2 (MH+).
Colorless crystals, ESI+ MS: m/z=466.2 (MH+)
Colorless crystals, mp. 107-108° C., ESI+ MS: m/z=391.3 (MH+)
The title compound is prepared in the same manner starting from 2-Amino-N-benzyl-N-methyl-2-phenyl-acetamide: Colorless crystals, ESI+ MS: m/z=379.2 (MH+)
N-Benzyl-2-(4-chloro-benzylamino)-2-phenyl-acetamide (730 mg, 2 mmol, example 8u), formaldehyde (1 ml, 37% aq. solution) are stirred for 2 hours at room temperature in a solution of dichloroethylene, methanol and acetic acid (6:3:1, 20 ml). Polymer supported cyano-borohydride (1 g, 4.3 mmol) is added and the mixture is stirred for 18 hours. The suspension is filtered, the filtrate is evaporated and the residue is purified by chromatography (CH2Cl2/MeOH, 99:1) to afford the desired compound as colorless crystals; 1H-NMR (400 MHz; DMSO-d6): 2.05 (s, 3H); 3.4 (dd, 2H); 4.08 (s, 1H); 4.3 (dd, 2H); 7.1-7.4 (m, 12H); 7.48 (s, 1H); 7.51 (s, 1H); ESI+ MS: m/z=379.2 (MH+).
N-Benzyl-2-(4-chloro-benzylamino)-2-phenyl-acetamide (730 mg, 2 mmol, Example 108) and triethylamine (348 μl, 2.5 mmol) is dissolved in methylenechloride (20 ml) and cooled to −20° C. Acetylbromide (163 μl, 2.2 mmol) in methylenechloride (1 ml) is added slowly and the resulting solution is stirred for 30 min. and is then warmed to 0° C. and stirred for another 90 min. The solution is warmed to room temperature and is stirred overnight. The mixture is washed with a diluted HCl solution (2N), dried and evaporated. The residue is purified by chromatography (CH2Cl2/MeOH, 99:1) and the enriched fraction is recrystallized from ether to afford the desired compound. ESI+ MS: m/z=407.2 (MH+)
The following compounds are prepared in accordance to the procedures as described in Example 115 starting with the appropriate starting materials:
Colorless crystals, mp. 176-178° C., ESI+ MS: m/z=368.2 (MH+).
Prepared from 2-Amino-N-benzyl-2-phenyl-acetamide (example 8a) and 3-Methoxycarbonylmethyl-benzofuran-6-carboxylic acid; mp. 129-131° C.
The following examples are prepared according to the procedure outlined in Example 44:
Colorless solid: m.p.=218.4-219.2° C. (dec.); HPLC: tR=6.13 min (gradient C), ESI+ MS:
m/z=407.1 (MH+).
Colorless solid: m.p.=215-216° C., HPLC: tR=6.54 min (gradient C), ESI+ MS: m/z=441.2 (MH+).
a) A DMA solution (1 ml) containing (9H-Fluoren-9-ylmethoxycarbonylamino)-(4-fluorophenyl)-acetic acid (55 mg, 0.14 mmol, 2 eq.), HATU (53 mg, 0.14 mmol, 2 eq.), and DIPEA (48 μl, 0.28 mmol, 4 eq.) is prepared and added to benzylamine-BAL resin (85 mg, 70 μmol, loading=0.8 mmol/g, LCC Reactospheres, LCC Engineering & Trading GmbH, CH-4622 Egerkingen, Switzerland) after 5 min of activation. The suspension is shaken overnight at room temperature. The solution is drained and fresh coupling solution (1 ml) is added and the mixture is shaken for further 3 hours. The resin is drained and washed with DMA (3×).
b) The resin obtained in step a) is suspended in 20% piperidine in DMA (1 ml) and shaken for 30 min. at room temperature. This procedure is repeated twice with fresh 20% piperidine in DMA (2×1 ml). Then, the resin is drained and washed with DMA (3×), isopropanol (2×), DCE (2×), NMP (2×).
c) A NMP solution (0.5 ml) containing 2-chlorobenzooxazole (107 mg, 0.7 mmol, 5 eq.) and triethylamine (97 μl, 0.7 mmol, 5 eq.) is prepared and added to the resin obtained in step b). The suspension is shaken overnight at 95° C. and the resin is washed with DMA (2×), DMA/H2O 7:3 (1×), isopropanol (2×), DCE (2×). Crude product is obtained after cleavage in 20% TFA in DCM for 3.5 hours at room temperature, and purified by preparative HPLC (gradient G). Lyophilisation with tert.-butanol gives the title compound as colorless powder. 1H-NMR (400 MHz, DMSO-d6): δ 4.25 (dd, J=15.3, 5.5 Hz, 1H), 4.35 (dd, 1H), 5.53 (d, J=7.9 Hz, 1H), 7.02 (t, J=7.3 Hz, 1H), 7.14 (t, 1H), 7.24 (m, 8H), 7.38 (d, J=7.9 Hz, 1H), 7.59 (dd, J=9.2, 5.5 Hz, 2H), 8.79 (d, J=8.6 Hz, 1H), 8.94 (t, J=6.1 Hz, 1H); HPLC: tR=5.5 min (gradient D), ESI+ MS: m/z=376.4 (MH+).
The following examples are prepared according to the procedures as described in Example 121.
HPLC: tR=4.0 min (gradient D), ESI+ MS: m/z=300.3 (MH+).
HPLC: tR=4.7 min (gradient D), ESI+ MS: m/z=326.3 (MH+).
HPLC: tR=6.3 min (gradient D), ESI+ MS: m/z=460.4 (MH+).
HPLC: tR=6.1 min (gradient D), ESI+ MS: m/z=349.4 (MH+).
HPLC: tR=3.9 min (gradient D), ESI+ MS: m/z=414.4 (MH+).
HPLC: tR=5.6 min (gradient D), ESI+ MS: m/z=323.4 (MH+).
HPLC: tR=5.6 min (gradient D), ESI+ MS: m/z=357.4 (MH+).
HPLC: tR=5.9 min (gradient D), ESI+ MS: m/z=403.5 (MH+).
HPLC: tR=5.7 min (gradient D), ESI+ MS: m/z=401.4 (MH+).
HPLC: tR=4.63 min (gradient E), ESI+ MS: m/z=497.7 (MH+).
To 2-amino-N-benzyl-N-BAL-2-phenyl-acetamide resin (160 mg, 0.128 mmol, loading 0.8 mmol g−1, prepared according to example 4a, steps a) & b)) is added a mixture of acetic anhydride, pyridine and DMA 1:1:8 (1.2 ml). The suspension is shaken for 10 min at room temperature. The solution is drained, fresh acylation solution (1.2 ml) is added and the suspension is shaken for further 10 min. The resin is then drained and washed with DMA, methanol, water, DCM, methanol, and DCM (2×). Crude product is obtained after cleavage in 20% TFA in DCM (2×1.5 hours), and purified by preparative HPLC (gradient G). Lyophilisation with tert.-butanol gives the title compound as colorless powder. HPLC: tR=4.0 min (gradient D), ESI+ MS: m/z=283.1 (MH+).
The following examples are prepared according to the procedures as described in Example 132.
HPLC: tR=4.3 min (gradient D), ESI+ MS: m/z=313.5 (MH+).
HPLC: tR=5.1 min (gradient D), ESI+ MS: m/z=367.5 (MH+).
The title compound is prepared by the procedure described in Example 99 starting from 3-tert-butoxycarbonylamino-3-phenyl-propionic acid: Colorless crystals, 1H-NMR (400 MHz; DMSO-d6): δ 2.5 (ddd, 2H); 3.45 (ddd, 2H), 3.95 (t, 1H); 4.28 (ddd, 2H); 7.0-7.4 (m, 14H); 8.35 (t, 1H), ESI+ MS: m/z=379.2 (MH+),
The title compound is prepared in the same manner as described in Example 99 starting from 2-tert-butoxycarbonylamino-3-phenyl-propionic acid, 2,2-diphenyl-ethylamine and pyridine-2-carbaldehyde: Colorless crystals, mp. 68-70° C., 1H-NMR (400 MHz; CDCl3): δ 2.65 (dd, 1H); 3.1 (dd, 1H); 3.2 (dd, 1H); 3.75 (m, 2H); 3.8-4.1 (abx, 2H); 4.2 (t, 1H); 6.85 (t, NH); 7.0-7.4 (m, 15H); 7.8 (t, 1H); 8.6 (d, 1H) ESI+ MS: m/z=436.2 (MH+).
To a solution of 10,11-Dihydro-dibenzo[b,f]azepine-5-carbonyl chloride (194 mg, 0.602 mmol, CAS 33948-19-5) in DCM (2 ml) is added benzylamine (145 μl, 1.32 mmol) and the mixture is allowed to stir at room temperature for 27 hours. The mixture is concentrated in vacuo and purified by chromatography on silica gel (hexane/ethyl acetate gradient 85:15-0:100). The title compound is obtained as a white solid. HPLC: tR=3.16 (gradient c), 1H-NMR (400 MHz; CDCl3): δ 2.70-2.90 (b, 2H), 3.30-3.50 (b, 2H), 4.44 (d, J=7 Hz, 2H), 4.89 (t, J=7 Hz, 1H); ESI+ m/z=329.2 (MH+).
The synthesis of the title compound is described in EP058373A1 (Ciba-Geigy AG). Colorless crystals: mp 282-283° C.
The synthesis of the title compound is described in EP058373A1 (Ciba-Geigy AG). Colorless crystals: mp 297-298° C.
The synthesis of the title compound is described in EP058373A1 (Ciba-Geigy AG). Colorless crystals: mp 97-98° C.
(4-Chloro-phenyl)-phenyl-methanone (21.5 g, 99 mmol), ethane-1,2-diamine (25 ml) are stirred for 20 hours at 180° C. A suspension of sodium borohydride (13 g) in water (30 ml) is added to a solution of the crude compound dissolved in methanol (300 ml). The mixture is refluxed for 1 hour. Water is added and the mixture is extracted with methylenechloride, dried and evaporated to afford 23 g of crude compound. Methanesulfonic acid is added to a solution of the crude compound in acetone. The resulting crystals are the dimethanesulfonate salt, mp 233-235° C., ESI+ MS: m/z=463 (MH+).
To an ice cooled solution of (S)-4-aminochromane (200 mg, 1.34 mmol) and triethyl-amine (207 μl, 1.47 mmol) in dry dichloro methane (5 ml) is added slowly oxalyl chloride (57 μl, 0.67 mmol) via a hypodermic syringe. Then, the light brown suspension is stirred for 3 h at room temperature under argon. After this, the reaction mixture is poured on ice water. The precipitate is filtered off, washed with cold water followed by diethyl ether. The residue is vacuum dried at 50° C. over night to give (S,S)-N,N′-di-chroman-4-yl-oxalamide as tan powder.
To a suspension of (S,S)-N,N′-di-chroman-4-yl-oxalamide (102 mg, 0.29 mmol) in dry THF (5 ml) is added slowly 1M borane-THF complex in THF (4.92 ml, 4.63 mmol) via a hypodermic syringe under argon. After the effervescence has ceased the now colorless thick suspension is brought to reflux and kept at this temperature over night. After reaching room temperature the colorless clear reaction mixture is poured on a mixture of ethyl acetate (25 ml) and water (25 ml). The organic layer is separated and the water phase extracted twice with ethyl acetate (10 ml). The combined organic extracts are washed with brine, dried over Na2SO4 and evaporated to dryness. The residue is purified by preparative HPLC (gradient F) to afford the title compound as a colorless powder, HPLC: tR=3.35 min (gradient A); ESI+ MS: m/z=295 (MH+).
N′1′-Benzhydryl-ethane-1,2-diamine (600 mg, 2 mmol) [prepared according to EP58373A1, Ciba-Geigy AG] is suspended in 10 mL DCM. To this mixture 1.4 mL triethylamine and 9-bromofluorene (540 mg, 2.2 mmol) are added and the reaction is stirred for 22 hours at room temperature. Subsequently DCM is added and the organic phase is washed with conc. NaHCO3 solution. The water phase is re-extracted with DCM. The combined organic phases are dried over Na2SO4, filtered and evaporated to dryness. The residue is purified by flash chromatography (cyclohexane/ethyl acetate, 1:1) to yield the title compound. Colorless crystals: mp. 89.0-91.1° C., ESI+ MS: m/z=391 (MH+).
Prepared in accordance to the procedures described in Example 143. Colorless resin HPLC: tR=6.55 min (gradient Acetonitrile+0.1% AcOH/water+0.1% AcOH, Acetonitrile from 0 to 100% in 8 min. 100% acetonitril for 2 min.; column: Waters XTerra® MS C18, 4.6×100 mm), ESI+ MS: m/z=419 (MH+).
Synthesis according to CH368493, Geigy AG, 24.06.1958. ESI+ MS: m/z=329 (MH+).
Synthesis according to CH368493, Geigy AG, 24.06.1958. mp. 172-173° C.
Synthesis according to W. Schindler, Helv. Chim. Acta; 37, 472, 481, (1954); mp. 278-280° C.
5000 soft gelatin capsules, each comprising as active ingredient 0.05 g of one of the compounds of formula I mentioned in the preceding Examples, are prepared as follows:
Preparation process: The pulverized active ingredient is suspended in Lauroglykol® (propylene glycol laurate, Gattefossé S. A., Saint Priest, France) and ground in a wet pulverizer to produce a particle size of about 1 to 3 μm. 0.419 g portions of the mixture are then introduced into soft gelatin capsules using a capsule-filling machine.
| Number | Date | Country | Kind |
|---|---|---|---|
| 0517740.7 | Aug 2005 | GB | national |
| Filing Document | Filing Date | Country | Kind | 371c Date |
|---|---|---|---|---|
| PCT/EP06/08426 | 8/29/2006 | WO | 00 | 2/29/2008 |
