Improved calcium phosphate cements are well tolerated by the body. These improved cements include a non-covalently bonded interpenetrating network.
Calcium phosphate composites are used as bone substitutes and bone grafts. These calcium phosphate composites tend to form complexes primarily between calcium-based salts through charge interactions. These composites are used as general bone void fillers and generally lack the adhesive strength sufficient to adhere or fix bones together, for example, fractured surfaces. These prior compositions have insufficient chemical interaction between the calcium phosphate composite and the bone surface or other surface materials and lack sufficient strength to be used to attach bone to bone or bone to other materials.
Certain marine species, such as tubeworms and sand castle worms, rely on secreted proteins containing a high amount of the amino acid phosphoserine for adhesion mechanisms (“The tube cement of Phragmatopoma californica: a solid foam,” Russell J. Stewart, James C. Weaver, Daniel E. Morse and J. Herbert Waite, Journal of Experimental Biology 207, 4727-4734, 2004). The specific mechanism of the phosphoserine involvement with the proteins is not understood; however, phosphoserine has been reported to be responsible for a specific interaction with calcium containing hydroxyapatite of bone as disclosed in U.S. Patent Application Publication No. 2005-0217538A1. In this publication, the authors mention calcium phosphate cements modified with phosphoserine (from 0.5% to 5% weight of the formulation) to aid as a compressive strength and surface area modifier in the bone cement material. In this range (from 0.5% to 5% weight of the formulation) the cement does not exhibit appreciable bone adhesion properties. In addition, certain bio-resorbable fibers have been used as adjuncts to calcium cements. These fibers include those based on polymers formed from mixtures of lactic and glycolic acids as well as other similar polymeric materials.
One aspect of the present invention relates to a non-covalently bonded interpenetrating network that comprises a reactive mixture of a small amino acid phosphate species, a multivalent metal compound, and a polymeric material that contain functional groups that contains electronegative atoms as the bonding sites of the polymer surfaces to the available metal ions, in an aqueous environment.
A further aspect of the present invention relates to a bone restorative composition that comprises (i) a multivalent metal phosphate compound, (ii) a polymer that contain esters, carbonyl, carbonate, carboxylic acids, amides, amine groups and mixtures thereof as the bonding sites of the polymer surfaces to the available metal ions, (iii) a compound of the formula;
where A is O, CH2, or S; R is H, NH2, NHCO(CH2)tCH3 where t is 0 to 2, NH(CH2)xCH3 where x is 0 to 3, NR1R2 where R1 is (CH2)yCH3 and R2 is (CH2)yCH3 where y is 0 to 2, (CH2)zCH3 where z is 0 to 3, where m is 0 to 1, and where n is 0 to 3 and wherein the compound is present in an amount greater than about 10% by weight based on the combined weight of the multivalent metal phosphate salt and the compound, and (iv) an aqueous medium.
A still further aspect of the present invention relates to kit for restoring bone comprising; A) A first container containing a composition that comprises: (i) a multivalent metal phosphate compound, (ii) a polymer that contain esters, carbonyl, carbonate, carboxylic acids, amides, amine groups and mixtures thereof as the bonding sites of the polymer surfaces to the available metal ions, and (iii) a compound of the formula;
where A is O, CH2, or S; R is H, NH2, NHCO(CH2)tCH3 where t is 0 to 2, NH(CH2)xCH3 where x is 0 to 3, NR1R2 where R1 is (CH2)yCH3 and R2 is (CH2)yCH3 where y is 0 to 2, (CH2)zCH3 where z is 0 to 3, where m is 0 to 1, and where n is 0 to 3 and wherein the compound is present in an amount greater than about 10% by weight based on the combined weight of the multivalent metal phosphate compound and the compound, and B) a second container containing an aqueous medium.
Another aspect of the present invention relates to a method of adhering a material to a polymer comprising the steps of: placing a composition between the material and the polymer wherein the composition comprises (i) a multivalent metal phosphate compound, (ii) a compound of the formula;
where A is O, CH2, or S; R is H, NH2, NHCO(CH2)tCH3 where t is 0 to 2, NH(CH2)xCH3 where x is 0 to 3, NR1R2 where R1 is (CH2)yCH3 and R2 is (CH2)yCH3 where y is 0 to 2, (CH2)zCH3 where z is 0 to 3, where m is 0 to 1, and where n is 0 to 3 and wherein the compound is present in an amount greater than about 10% by weight based on the combined weight of the multivalent metal phosphate salt and the compound, and (iii) an aqueous medium; and allowing the composition to cure to form an interpenetrating network at the interface between the composition and the polymer, wherein the polymer contain esters, carbonyl, carbonate, carboxylic acids, amides, amine groups and mixtures thereof as the bonding sites of the polymer surfaces to the available metal ions, and wherein the composition adheres to the material.
Other aspects and advantages of the present invention will become apparent upon consideration of the following detailed description.
Small molecule multivalent metal compounds such as calcium phosphates, including tetracalcium phosphate (TTCP) react with small molecule organophosphate compounds such as amino acid phosphate compounds like phosphoserine to form cements in the presence of aqueous environments that have cohesive and adhesive properties. When these cements are in the presence of polymer materials, the multivalent metal compounds and the amino acid phosphate compounds form a complex, interpenetrating network with the surface of polymer materials. The significance of these interpenetrating networks is the ability to form durable materials with high intrinsic strength (e.g. energy to failure) and in some cases extrinsic strength (e.g. adhesion to polymeric surfaces). The polymeric material added to the formulation to form high intrinsic strength can be in any form, such as solution, powder, fiber, resin, liquid crystal, hydrogel, chip, flake, sheet, mesh, and the like. The polymeric material to which the formulation can adhere can be in any form, such as a plate, sheet, mesh, screw, pin, anchor, thread, fiber, suture, foam, film and the like. The complex formation of the interpenetrating network system involves several modes of ionic or ion-dipole interactions stemming from the release of multivalent metal ions such as calcium, magnesium, barium, strontium, and titanium ions and mixtures of these ions from compounds like calcium phosphate, magnesium phosphate, barium phosphates, strontium phosphate, titanium phosphate, zirconium phosphate, calcium oxide, magnesium oxide and mixtures thereof.
Certain small molecule amino acid phosphate compounds, such as phosphoserine, have a phosphate group (PO4), a carboxyl group (COOH), and an amine group (NH2) which are all capable of forming ionic interactions with the available metal ions. For rapid and abundant interactions, TTCP is the ideal metal ion source since it has the highest calcium to phosphate ratio (2:1) of the known calcium phosphate compounds and is well tolerated by the body. Basic TTCP is a calcium rich small molecule that is highly strained and dynamic. As it reacts in an acidic environment, the structure opens to release the calcium ions for ionic bonding. When it releases the calcium, the phosphoryl oxygen of the phosphate group of the TTCP intermediate is available for additional calcium ionic bonding. On this basis the authors hypothesize one method is to manufacture a calcium rich molecule with a calcium to phosphate higher then 2:1 which is even more reactive compared to TTCP. In addition, compositions with less reactivity can also be suitable for use. Such compositions could utilize calcium phosphate compounds with a calcium to phosphate ratio less than 2:1, such as alpha-tricalcium phosphate (1.5:1) or compositions could utilize calcium based compounds which are not from the calcium-phosphate family, such as calcium chloride or calcium oxide. It is preferred that the multivalent metal compound be non-toxic as many uses of these compositions are for medical and/or veterinary uses. However, if the cement is not to be used relative to living organisms, toxicity is of less concern. Suitable multivalent metal compounds include: calcium phosphate, magnesium phosphate, barium phosphates, strontium phosphate, titanium phosphate, zirconium phosphate, calcium oxide, magnesium oxide and mixtures thereof. In addition, non-phosphate compounds of these metals such as carbonates, bicarbonates, sulfates and nitrates can replace some or all of the above phosphate compounds.
To establish a fully integrated interpenetrating network system, ion-dipole multivalent metal ion interactions develop and are extended to the polymer surfaces. For example, with TTCP and phosphoserine, the electronegative carbonyl oxygen atom(s) of the ester group or the electronegative nitrogen atom(s) of the amine group are the bonding sites of the polymer surfaces to the available calcium ions. The reactive and charged TTCP/phosphoserine complex that develops influences the folding of the adjacent polymer chains on the polymer surface in a favorable geometry to enhance the ion-dipole bonding. In addition, both the hydrophobicity and steric structure of the polymer backbone influences the degree of interaction between the polymer to the TTCP/phosphoserine complex. This geometry is depicted in
It has further been found that certain multivalent metal compound cements that include a certain minimum amount of small amino acid phosphate compounds of the formula;
where A is O, CH2, or S; R is H, NH2, NHCO(CH2)tCH3 where t is 0 to 2, NH(CH2)xCH3 where x is 0 to 3, NR1R2 where R1 is (CH2)yCH3 and R2 is (CH2)yCH3 where y is 0 to 2, (CH2)zCH3 where z is 0 to 3, where m is 0 to 1, and where n is 0 to 3 have superior strength in the presence of polymeric compounds that have ionically available electron pairs compared to known calcium phosphate or multivalent metal compound cements. Preferred adjunct compounds are those where A is O or CH2, R is H or NH2, m is 0 or 1 and n is 0 or 1. It is also possible that other similar materials that can assist the multivalent metal ionically bond to reactive sites on the resorbable polymer materials are usable in the calcium phosphate mixture. At present one preferred species is phosphoserine.
The compositions as described herein are useful in a wide variety of medical applications. One use of the compositions is to adhere bone fragments together within the body. This is useful, for example, during surgery to allow for temporary fixation prior to definitive hardware placement, and to enhance fracture fixation by adhering both load and non-load bone fragments together alone and/or in the presence of appropriate immobilization. The compositions also enhance screw and/or bone anchor fixation into low density cancellous bone at and/or after surgery, to allow screw fixation when the core diameter of the screw hole is larger then the screw major diameter, for instance to reattach a metal or bioresorbable screws to bone that has stripped from the surrounding material, to adhere a metal or bioresorbable plate to fractured bones allowing for reduction and/or elimination of metal or bioresorbable screws or pins used to fix a metal or bioresorbable plate to bone. The use of the compositions with a bioresorbable suture may be used to help fixate small bones after fracture. The compositions also have the capacity to enhance fixation of a joint replacement prosthesis to bone (e.g. hip acetabular cup or femoral stem). The compositions adhere the junction of at least one of a tendon, ligament, cartilage, a bone graft, and/or dental implants to bone. The compositions may be used to support new bone growth for dental socket or dental ridge augmentation. The compositions have the capacity to adhere to bony defect perimeters while filling gaps creating a seal to prevent leakage (e.g. cerebral spinal fluid). Furthermore, the compositions may also be used in ossicular chain reconstruction to adhere middle ear ossicles together. The adhesive properties of the compositions of the present invention to bone and bone to other materials make them useful to provide bony contour for facial bone augmentation applications. These compositions are also useful for gluing cancellous bones, cortical bones and a combination of both, whether in fatty or greasy environments potentially without any surface pretreatment prior to application.
One particularly novel use of the compositions is as a bone restorative composition. By a bone restorative composition, it is meant a composition that is useful to restore and/or repair bone, such as bone adhesives, bone cements, bone glues, bone putties, bone void fillers, bone replacement compositions, cements and/or adhesives to fix screws, metal or bioresorbable implants and at least one of a tendon, ligament, cartilage, a bone graft, and/or a dental implant to bone.
As noted above, the compositions have a tacky state shortly after initial mixing. This tacky state enables at least two items, such as two pieces of bone, bone and another material, or two non-bone materials to be held together by the composition itself, without the need for external force, until the composition sets to the final hardened cement state. The amount of force needed to remove two opposed pieces of material from each other is the separation strength. For the composition as described herein, these compositions have a separation strength during the tacky state within the first 4 minutes and preferably within the first 2 minutes after initial mixing from about 10 kPa to about 250 kPa and preferably from about 50 kPa to about 150 kPa. For certain applications it may be useful to have a longer tack state whereby certain compositions have a separation strength which continues in this range for up to 12 minutes. This separation strength is sufficiently high that the items to be joined need not be held together unless there is an apposing strength of the items greater than the separation strength and also, the items can still be repositioned or even reapposed without loss of ultimate bond strength.
It has been found that in the present compositions tetra calcium phosphate (TTCP) has unusual properties not shared by other calcium phosphate compositions or other multivalent metal compounds. TTCP is the most basic of all the calcium phosphates; therefore, it readily reacts to acidic compounds. While other calcium phosphate compositions can be used in addition to the TTCP, it is preferred that the compositions include an effective amount of TTCP. The TTCP used in the present compositions can be made by a variety of methods. One such manufacturing method is disclosed by Chow and Takagi in U.S. Pat. No. 6,325,992, the disclosure of which is hereby incorporated by reference. The TTCP can be 100% pure material or can include other calcium and calcium phosphate materials as an impurity, e.g. α-TCP, CaO and/or HA.
Typical amounts of multivalent metal compounds are from about 10% to about 89.9% by weight based on the total composition of the dry ingredients, namely the metal compound, the small amino acid phosphate species and the polymer. A preferred and optimum amount of the divalent metal compound is from about 40% to about 80% and about 55% to about 65% by weight on the same basis.
A second component of the compositions is a small amino acid phosphate species that has the following formula;
where A is O, CH2, or S; R is H, NH2, NHCO(CH2)tCH3 where t is 0 to 2, NH(CH2)xCH3 where x is 0 to 3, NR1R2 where R1 is (CH2)yCH3 and R2 is (CH2)yCH3 where y is 0 to 2, (CH2)zCH3 where z is 0 to 3, where m is 0 to 1, and where n is 0 to 3. Preferred compounds are those where A is O or CH2, R is H or NH2, m is 0 or 1 and n is 0 or 1.
The most preferred small amino acid phosphate species is phosphoserine that has the following structure;
The compounds that are structurally similar to phosphoserine, which contain the reactive phosphonate or phosphate, and which have COOH functional groups, are capable of interacting with the Ca++ within the TTCP to form a calcium based matrix and are referred to as compounds structurally similar to phosphoserine in this description. The combination of these functional groups plus the geometry properties of the matrix such as the chain length between the phosphorous and the COOH are unique aspects to the molecules which affect the level of adhesive bonding strength to substrate surfaces such as bone and metal.
The preferred compound that is structurally similar to phosphoserine is any form of phosphoserine, including the phospho-D-serine, phospho-L-serine or the phospho-DL-serine, and/or other similarly constructed compounds. The exact stereochemistry of the phosphoserine does not seem to have any impact on the properties of the compositions disclosed herein.
It has been found that when the quantity of the small amino acid phosphate species are included in the mixture and are increased beyond about 10% w/w based on the total composition of the dry ingredients, namely the metal compound, the small amino acid phosphate species and the polymer, more generally in the range of about 10% to about 89.9%, more typically in the range of about 15% to about 50%, or preferably from about 20% to about 40%, the tack and adhesion properties of the resulting compositions were significant. At such levels, the influence of the small amino acid phosphate species extends beyond internal interaction with the cement, but also extends to significant binding with the hydroxyapatite architecture and proteins of bone. At below about 10% by weight of the small amino acid phosphate species, the compositions do not have a tacky state and these compositions do not have adhesive properties.
Factors that may affect the length of the tacky state, the length of the putty states and the ultimate cure time, as well as strength properties of the compositions include: the percentage (w/w) multivalent metal compound and the small amino acid phosphate species based solely on the weight of the multivalent metal compound and the small amino acid phosphate species in the composition, the selection of the small amino acid phosphate species, the particle size of the multivalent metal compound, and the nature and quantity of any additives and/or fillers which may be combined to the composition to enhance the material properties.
The mean particle size of the multivalent metal compound should be below about 1000 μm, preferably about 1-250 μm, most preferably about 10-100 μm. As the mean particle size of the multivalent metal compound is reduced, the multivalent metal compound tends to dissolve too fast and these compositions may not be practical for all uses as disclosed herein. On the other hand if the multivalent metal compound has a mean particle size of greater than about 1000 μm, the intra-operative performance of the compositions may not have the desired initial strength and be too slow to set. If a longer working time is desired, then multivalent metal compound with a larger mean particle size can be used; however, if a shorter working time is desired, then multivalent metal compound with a smaller mean particle sizes can be used. In certain use environments, compositions that have a multi-modal mean particle size distribution with, for example, one mode less then about 50 μm and the other mode above about 50 μm can provide unique properties such as a fast initial cure rate from the smaller mean particle size mode combined with higher intrinsic compression strength of the material from the larger mean particle size mode.
The aqueous based mixing media useful for combining the multivalent metal compound and the small amino acid phosphate species powders can include water, buffers such as sodium phosphate, saline, and blood based products such as whole blood, plasma, platelet rich plasma, serum, and/or bone marrow aspirate. The blood based products are used with the goal of achieving enhanced rate of bone healing and remodeling. It is also possible to use the compositions without premixing with an aqueous medium if the composition is to be used in a sufficiently wet environment that the aqueous medium can be absorbed from the in situ site. In this situation, the composition can be dusted on and/or other wise applied to the desired site and then mixed with the liquids that are already present at the site. The amount of aqueous medium is not particularly important other than the amount should be chosen to provide the consistency of the desired product for use as a bone restoration composition or other use.
Suitable polymers must contain functional groups that contains electronegative atoms as the bonding sites of the polymer surfaces to the available metal ions, such as electronegative carbonyl oxygen atom(s) of the ester group or electronegative nitrogen atom(s) of the amine group as the bonding sites of the polymer surfaces to the available calcium ions. These functional groups can be either in the backbone chain of the polymer or in groups that are pendant to the polymer chain. These polymeric based compounds can include, but are not limited to, one or more of the following; poly(L-lactide), poly(D,L-lactide), polyglycolide, poly(ε-caprolactone), poly(teramethylglycolic-acid), poly(dioxanone), poly(hydroxybutyrate), poly(hydroxyvalerate), poly(L-lactide-co-glycolide), poly(glycolide-co-trimethylene-carbonate), poly(glycolide-co-caprolactone), poly(glycolide-co-dioxanone-co-trimethylene-carbonate), poly(tetramethylglycolic-acid-co-dioxanone-co-trimethylene-carbonate), poly(glycolide-co-caprolactone-co-L-lactide-co-trimethylene-carbonate), poly(hydroxybutyrate-co-hydroxyvalerate), poly(methyl-methacrylate), poly(acrylate), polyamines, polyamines, polyimidazoles, poly(vinyl-pyrrolidone), collagen, silk, chitosan, hyaluronic acid, gelatin and mixtures thereof. In addition, copolymers of the above homopolymers also can be used. The general structural nature of polymers include linear homo and copolymers, cross linked polymers, block polymers, branched and hyper branched polymers and star shaped polymers. The polymers can be added to the formulation in the form of a solution, powder, fiber, resin, liquid crystal, hydrogel, chip, flake, and the like. The polymeric material can be included directly within the cement formulation or can be an adjunct that is applied in situ as the cement is applied to the bone. The only import aspect is that sufficient polymeric material is available to ionically bond with the calcium phosphate and the adjunct material as described below.
The polymeric material that is included within the composition should be sufficient to show a difference in intrinsic material strength (e.g. the bending moment energy to failure). That amount should be of sufficient weight within the formulation; amounts greater than about 0.1% w/w based on the total weight of the dry components of the composition would be sufficient. The polymeric material included within the composition can be up to about 30% w/w based on the total weight of the dry components of the composition; however, as such levels the adhesive properties decrease; therefore, a balance between intrinsic strength and material adhesive properties is required. For polymeric materials in contact with the composition it is important that the surface area of the polymer is readily available at the molecular level or is in direct contact with the composition.
It has been confirmed as noted above that the polymers form an interpenetrating network having a structure similar to what is shown in
The interpenetrating networks can be formed at both an acidic and a basic pH. However, only when the pH is raised to a level above pH of about 5 and preferably above a pH of about 6 so the interpenetrating networks form permanent bonds. Also the pH should typically be below a pH of about 9 to be tolerated by the body. If the materials are to be used for other purposes, there is no upper limit other than a pH that will degrade the polymeric material.
Additives may be included in the compositions disclosed herein to further enhance the material properties. These properties include the handling, porosity, intrinsic material strength, & bone healing rate (osteogenic). Depending on the multivalent metal compound chosen, suitable additives might include: alpha or beta tri-calcium phosphate (α-TCP or β-TCP), calcium sulfate, calcium silicate, calcium carbonate, sodium bicarbonate, sodium chloride, potassium chloride glycerol phosphate disodium, amino acids such as serine, excess amounts of phosphoserine, polyols (such as glycerol, mannitol, sorbitol, trehalose, lactose, & sucrose), silk, keratin (primarily found in human hair), autologous bone powder or chips, demineralized bone powder or chips, collagen, BMP7, stem cells, parathyroid hormone (PTH), bisphosphonates, and mixtures thereof. In addition, other additives and/or fillers could be incorporated which offer surgical visual aids & anti-infective properties.
The α-TCP and β-TCP additive component typically is also in granular form. The granules presently contemplated have an overall diameter size in the range of about 0.1 to about 2 mm, or preferably between about 0.5 to about 1 mm. Larger and smaller granules can be used depending on the other components of the composition and the desired end properties. In the present compositions, the particle size of the granules has an impact on the mechanical strengths of the resultant compositions. The total porosity of these granules is in the range of about 40-80%, more preferably about 65-75%, and the average pore diameter size of the granules in these compositions is in the range of about 20-500 μm, preferably about 50-125 μm. The granules do not dissolve within the present embodiments during the curing phase, but interact as a solid particle with the other components of the compositions. In the present compositions, the porosity and pore size listed here has an impact on the resorption characteristics of the resultant compositions and to allow for bony in growth and healing as described by Dalal et al. in U.S. Pat. No. 6,949,251.
The additives that affect the porosity include cement curing pore forming agents such as calcium carbonate or sodium bicarbonate, granules with pre-formed pores made from alpha or beta tri-calcium phosphate (α-TCP or β-TCP), biodegradable polymers usually in fiber form that open channels or pores as they degrade relatively quick in vivo such as PGA, or copolymers such as PLGA, or biodegradable fibers that open channels or pores as they degrade over relatively long time periods such as PLLA, silk, keratin, collagen, autologous bone powder or chips, or demineralized bone powder or chips. The rate at which the biodegradable polymers degrade can be dependant on the physical state of the crystalline structure when processing the polymer. Amorphous polymers may resorb faster than crystalline polymers. Other biodegradable polymers not in the form of fibers, rather powders, can be used such as PLLA, PGA, PLGA, PEG, or block polymers such as PLLA-PEG-PLLA. Small molecules may also be used which leach away relatively quickly from the cement as it cures; for example, these materials may include sodium chloride, potassium chloride, glycerol phosphate disodium, polyols (such as glycerol, mannitol, sorbitol, trehalose, lactose, & sucrose), amino acids such as serine, and/or excess amounts of phosphoserine. Other materials that form pores may dissolve or resorb over time in vivo and release from the cement opening pores; these materials include calcium sulfate, α-TCP or β-TCP powder or granules. Granules can be used to alter the in vivo resorption profile, such as α-TCP or β-TCP granules, or hybrid granules made from calcium sulfate and α-TCP or β-TCP in which the calcium sulfate portion resorbs more quickly.
The additives that affect the bone healing rate driven by new bone ingrowth can be influenced by the level of porosity of the cured cement. This rate can be manipulated by the number of pores and size of the pores created within the cured cement. Achieving such porosity up to about 60% v/v was demonstrated by controlling the ratio of composition ingredients. The porosity that develops during the curing process can be controlled by the amount of pore forming agent added (such as calcium carbonate), the level of compound structurally similar to phosphoserine added, the level of aqueous solution used, and/or the level of other agents added to the composition. Increasing the porosity reduces the material intrinsic strength; however, a balance of porosity vs. strength is critical for achieving the clinical application. Additives that increase the intrinsic material strength can be incorporated to offset the loss of strength by creating porosity.
These polymeric materials can be supplied as fibers into the composition to increase the material intrinsic strength. An important aspect for chemical ion-dipole adhesion of these fibers is the size and/or surface area. The size or surface area can be defined by the aspect ratio (length:diameter). The preferred aspect ratio is from 2:1 to 50:1; more preferable from 10:1 to 35:1. The overall length of the fiber can be up to 5 mm; however, since the material could be used as bone to bone adhesive, the length of the fiber may be more appropriate at lengths up to 2 mm.
The additives that act as visual aids in the surgical procedure include colorants such as a pigment or dye to aid in determining coverage and depth of the applied cement or contrast agents such as barium salts in determining depth on a radiograph.
Other additives can be incorporated into the compositions that enhance the bone healing rate (osteogenic) These additives comprise a class of osteogenic growth factors including bone morphogenetic proteins (BMP's), such as BMP 7, stem cells, parathyroid hormone (PTH) and/or anti-osteoporotic agents such as bisphosphonates can be contemplated for incorporation into the composition.
Other additives that can be incorporated into the composition are infection preventatives such as broad spectrum antibiotics and anti-infectic additives.
The compositions as described herein have many unique properties not found in prior calcium phosphate compositions. One particularly important property is that the compositions have a tacky state immediately subsequent to mixing with an aqueous medium. This tack property is retained for a number of minutes, sometimes up to about 12 minutes depending on the application requirement, typically up to about 4 minutes, and preferably up to about 2 minutes, after mixing with the aqueous medium. The time of the tacky state is dependent on a number of factors including relative ratio of the components, the particle sizes of the component materials, the presence of additives and the like. During this time the compositions will adhere bone to bone and bone to other materials, often without the need for external clamping or other application of pressure. The tacky state is not so aggressive that the composition will permanently affix the materials together at this point in time. Rather the tacky state can allow the materials to be moved relative to each other and also to be re-opposed without appreciable loss of ultimate cured strength. This is important in a medical setting so that the user can make sure the bone and the other material to be adhered to the bone are in the proper position relative to each other.
The tacky state is followed by a putty state. In the putty state, the tacky property has substantially disappeared and the compositions can be shaped or sculpted. In addition, during the putty state, the composition can be formed into shapes or used to fill voids in bone in a manner similar to putty. This putty state is retained for a number of minutes, sometimes up to 15 minutes depending on the application requirement, typically up to about 8 minutes, and preferably up to about 5 minutes, after mixing with the aqueous medium. Like the tacky states, the putty state is dependant on a number of factors including the relative ratio of the components, the presence of additives, the particle size of the components and the like. Because the items to be affixed can be repositioned during the tacky state or the compositions can be shaped during the putty state, this combined time of the tacky state and the putty state is some times referred to as the working time. Typical compositions have a working time of up to about 8 minutes from initial mixing and often the working time is up to about 5 minutes after which time the compositions have sufficiently begun hardening that further manipulation will result in degradation of ultimate strength of the bond.
After the putty state, the compositions harden like a cement to form a substantially permanent bond between the materials. In the cement state, the composition hardens and the materials that have been affixed to each other cannot be separated without the application of significant force. The compositions typically will begin to harden within about 8 minutes, and often within about 5 minutes, after mixing with the aqueous medium. The amount of time to reach the cement state is also dependant of the same factors listed above.
A further important property of the compositions is that these compositions have significant coherency and integrity within a wet environment. In the medical field, this would include a surgical site and a wound or similar situation where blood and other bodily fluids are present. The tacky state, the putty state, and the cement state all occur in either a wet environment or in a dry environment. In order to get the desirable properties, the user need not ensure that the application site is clean and dry. In a wet environment, the compositions tend to remain together and the presence of the liquid does not significantly affect the integrity of the composition or the ultimate strength properties.
While not wishing to be bound by theory, compositions of the present disclosure are believed to function as follows: the TTCP, which is basic in nature, reacts with the small amino acid phosphate species, which is acidic in nature, upon mixing with the aqueous medium and forms a hardened, layered structure upon curing. This reaction is exothermic; the degree of exothermic activity depends on a number of factors including the volume of the composition. The low pH nature of the compounds that are structurally similar to phosphoserine enable the hydroxyl of phosphate or phosphonate and COOH functional group to bond through ionic interaction with the calcium ions from within the TTCP. This resulting reactive intermediate continues a cascade of ionic interactions with calcium and phosphate ions within the TTCP or HA on the bone surface or any other metal ions of the metal implants. This series of interactions provides transient material having the tacky properties while curing and the adhesion strength that increases upon cure.
The exothermic properties of the composition when curing are prevalent when mixing as a large volume bone void filler (usually greater then 10 cc) and this may serve as an effective means to kill the residual tumor cells locally that remain after surgical bone tumor removal.
The exothermic properties of the composition may lead to necrosis of local tissue and this also reduces the adhesive working time. The amount of heat released by the exothermic reaction is mainly influenced by the volume of the composition, the size of the particles and the ratio of compound that is structurally similar to phosphoserine to TTCP. With larger volumes of composition, more heat is released to the surrounding tissue. With volumes less than or equal to about 1 cc, the heat release is negligible with maximum temperature reached during the curing of the adhesive being below about 40° C. The higher volume compositions greater than about 1 cc, led to considerable heat release, even exceeding about 60° C. in compositions greater than about 5 cc. To manage this exothermic heat release to below about 45° C., the particle size distribution of the TTCP, and the ratio of TTCP to compound that is structurally similar to phosphoserine can be chosen appropriately. The smaller TTCP particles dissolve and react faster due to a higher specific surface area; therefore, to reduce the exothermic heat release, the composition can be adjusted by choosing a TTCP particle size distribution which generally has a mean particle size greater than about 15 μm, more specifically about 25 μm. In addition, the greater amount of TTCP to the compound that is structurally similar to phosphoserine used, the faster the reaction occurs due to the number of calcium ions available for bonding. Exothermic heat release can be limited by adding more compound that is structurally similar to phosphoserine to the composition. To further reduce the exothermic heat release, endothermic additives can be incorporated into the composition to slow the reaction rate; these include polyols (such as sorbitol or mannitol) and/or PEG. The factors discussed here can be chosen to design several compositions; all of which have exothermic profiles which limit or eliminate necrotic reactions to local tissues while tailoring the compositions with sufficient working time for the clinical application.
The compositions when mixed with aqueous medium typically have a creamy or a tacky paste consistency initially. Also, the mixing of the compositions with the aqueous medium does not require a high level of force or shear. Generally, simple hand mixing, such as with a spatula, is sufficient in most instances. It is envisioned that the present compositions may be applied via injection through a syringe or other suitable pressurized implement, applied with a spatula, and as otherwise desirable by a user. The creamy or tacky viscosity allows for application of the composition to the defect site for a defined period of time. The compositions allow the bone to be repositioned several times within about 4 minutes and preferably within about 2 minutes without losing tack properties. If the compositions need to be injected through a syringe or cannula, the viscosity of the composition during the working time can be important. For these situations, viscosities of the compositions herein should be preferably below about 150 centipoise.
Still further embodiments have a consistency similar to putty. These embodiments are useful for filling larger defects, have sculpting properties, or for mechanical interlocking into cancellous bone. These compositions hold their cohesive, tacky, and sculpting properties over a longer period of time even when subjected to a wet field. The compositions have working time for sculpting sometimes up to about 15 minutes depending on the application requirement, typically up to about 8 minutes, and preferably up to about 5 minutes, after mixing with the aqueous medium. Formulations with an increased amount of small amino acid phosphate species greater than about 25% w/w or increased TTCP mean particle size greater than about 250 microns tend to have longer working times and seem to be suitable for use in situations were the putty will fill defects in structures that are well supported by surrounding bone. In these situations the putty does not need to harden as fast provided it maintains its cohesive properties in the wet field. Another property of the compositions is that the compositions will adhere to themselves as well as to an external surface such as bone. This is useful in situations where a shape is formed during the putty state and this shape can then adhere to bone. Also, in some instances a user may apply a mass of the composition to a bone or other surface and then shape the composition into the final desired shape during the working time of the composition.
Compositions which have a putty consistency to be used a void filler can be enhanced by incorporating macro porous granules or chips to allow for new bone ingrowth. These granules may come from synthetic sources such α-TCP or β-TCP granules or it may be preferred to select the granules or chips from autologous bone sources or demineralized bone to enhance the bone healing rate.
It is further envisioned that the cement compositions disclosed herein may be packaged into kits that may include a vial containing the TTCP with the small amino acid phosphate species pre-filled together and packaged under vacuum, nitrogen, or dry air to preserve the shelf life. Further, if additives are used, they may be included within this vial or in a separate vial. The aqueous medium is provided in a separate vial. The kit may include mixing bowls, stirring sticks, spatulas, syringes, and/or any other desirable component for application.
Composition of the current disclosure are envisioned to provide ease of use in different medical applications based on ease of application, duration of use before cure, resistance to in vivo environments, extended maneuverability of bone fragments and/or implant devices prior to cure onset, good load bearing capabilities after cure, and good ultimate bond strength. For example, compositions may have an adequate working period after mixing sometimes up to about 15 minutes depending on the application requirement, typically up to about 8 minutes or less, and preferably up to about 5 minutes or less. Further, the relative force of pressure required to inject the composition from an appropriately sized syringe may remain constant or below a certain injection force threshold from the point of mixing and loading the syringe to the end of the working period. It is contemplated that bone fragments adhered together or implanted devices may exhibit moderate separation strengths within the working period. Such moderate separation strengths may be exhibited regardless of the relative compressive force used during apposition. It is further contemplated that cement compositions of the present disclosure may have sufficient material cohesion when applied in moist, wet, greasy and/or fatty saline environments, such as in vivo settings, thereby reducing the need for surface preparation and maintaining a dry environment. As well, good capacity for supporting passive movement and maintaining load and non-load bearing bone fragment alignment after surgery during initial rehabilitation period and active range of motion rehabilitation period are envisioned for cement compositions contemplated herein.
Typical compositions exhibit an adhesive strength upon curing, typically after greater than about 10 minutes from initial mixing, in the range of about 250 to about 2000 kPa on cancellous bone and from about 250 to about 10,000 kPa on cortical bone in at least one of compression, tension, shear, and/or bending. Compositions can be chosen to achieve the strength in these ranges; the level of strength required is dependent upon the clinical application. Also, it is important to note that the curing can be accomplished either in a wet environment, such as in bodily fluids, or in a dry environment, and the ultimate strength of the bond after cure does not seem to be significantly affected.
In the following examples all shear, tension and bending testing was done using an Instron Force test machine (Model #5564) setup as follows. For shear testing, the sample was supported and fastened to the machine at one end of the sample and the other end was left free and unsupported. For shear testing the samples have a bond surface that was 90° to the face of the bone samples unless there was an indication that the bond surface was at an angle of 45° from the face of the bone surface. The force test probe was placed in plane against the top of the bond line of the sample and force was applied until failure. For tension testing each end of the sample was clamped to the testing machine and the force was applied at 90° to the bond to pull the sample apart. When the bond fails the result was recorded. For the 3 point bending testing each end of the sample was supported without clamping the sample to create a span distance of 35 mm. Force was applied by the force probe to the top of the sample at the center point (same position as the bond line) between both ends until the bond fails. The TTCP that was used in all the following examples was a commercially available material that included from about 17% to 32% of related impurities. These materials all contained about 68% to 83% TTCP.
A cement formulation having the following formulation: 250 mg OPLS, 400 mg TTCP, and 133 μl of water was mixed and applied to a 10 mm×10 mm×2 mm thick sheet of either 10/90 PLGA or 50/50 PLGA as shown in the table and to a cortical bone cube. Before strength testing the samples were allowed to cure for 10 minutes submersed in water at 30° C. The test was repeated 6 times and compared to two commercially available calcium phosphate based bone cements that were mixed in accord with label directions.
As shown in
A cement formulation having the following formulation: 250 mg OPLS, 400 mg TTCP, and 133 μl of water was mixed and applied between two 10 mm×10 mm×2 mm thick sheets of either 10/90 PLGA or 50/50 PLGA as shown in the table. Before strength testing the samples were allowed to cure for 10 minutes submersed in water at 30° C. The test was repeated at minimum 6 times and compared to two commercially available calcium phosphate based bone cements that were mixed in accord with label directions.
As shown in
In order to see the effect on the intrinsic material strength, polymer fibers were added to the compositions to form an interpenetrating network. A series of compositions set out in Table 1 were prepared and tested as follows. Each composition was mixed for 20 seconds in a polycarbonate bowl using either a polycarbonate pestle or spatula. After mixing, the composition was applied to both surfaces of bovine cortical bone cubes that had apposing faces using a spatula. The faces were created with a 90° angle for the bending tests (9×9.5 mm face). Prior to testing, the bone cubes were incubated within a phosphate buffered saline (PBS) solution bath at 30° C. and had pre-dampened surfaces during composition application. By 90 seconds from the start of mixing, the apposing faces were adhered together and aligned with minimal hand compression force for 10 seconds and were immediately transferred and submerged within a PBS solution bath held at 30° C. for the duration of 10 minutes. After the samples cured, the cubes were loaded onto the 3 pt bending sample fixture and tested on an Instron force test machine. The compositions that form an interpenetrating network with polymer fibers (3A & 3B) show increased bending strength compared to the fiber free composition (Comparative Example 3) that does not form an interpenetrating polymer network system.
The procedure of Example 2 was repeated except that the PLGA sheets were replace by polycarbonate and acrylic sheets. The surface treatment on these sheets was removed by rubbing the surface with the edge of a glass slide. The polycarbonate sheet was clear polycarbonate sheet 0.236 inches thick (part 8574K11) and the acrylic sheet was clear 0.25 inch thick acrylic sheet (part 8560K354), both available from McMaster-Carr, Aurora, Ohio. An attempt was made to test the Norian cement as was done in Example 2 however the Norian cements displayed no adhesion to the polycarbonate or acrylic sheets and the cement disintegrated over time in a wet environment. The test was repeated at minimum 6 times.
Cement compositions disclosed herein provide adhesive and cohesive strength through mechanical and chemical interlocking with bone substrates. The cement formulations have a tack and/or sticky quality that allows temporary adherence early in the curing process and a delay in significant curing early on. The cement formulations may mimic natural bone architecture and provide superior mechanical strength over longer periods of time relative to convention cement formulations.
The disclosure has been presented in an illustrative manner in order to enable a person of ordinary skill in the art to make and use the disclosure, and the terminology used is intended to be in the nature of description rather than of limitation. It is understood that the disclosure may be practiced in ways other than as specifically disclosed, and that all modifications, equivalents, and variations of the present disclosure, which are possible in light of the above teachings and ascertainable to a person of ordinary skill in the art, are specifically included within the scope of the impending claims. All patents, patent publications, and other references cited herein are incorporated herein by reference.
The present application is a division of U.S. patent application Ser. No. 14/980,022, filed Dec. 28, 2015, which is a continuation of U.S. Pat. No. 9,265,857, filed May 10, 2011, which claims the benefit of U.S. Provisional Application No. 61/333,478, filed May 11, 2010. The entire contents of each are incorporated herein by reference.
Number | Name | Date | Kind |
---|---|---|---|
3899556 | Heide et al. | Aug 1975 | A |
4183915 | Gaffar et al. | Jan 1980 | A |
4234972 | Hench et al. | Nov 1980 | A |
4373217 | Draenert | Feb 1983 | A |
4491987 | Park | Jan 1985 | A |
4563432 | Ehlert et al. | Jan 1986 | A |
4563489 | Urist | Jan 1986 | A |
4596574 | Urist | Jun 1986 | A |
4600546 | Grundei | Jul 1986 | A |
4610692 | Eitenmuller et al. | Sep 1986 | A |
4629464 | Takata et al. | Dec 1986 | A |
4634720 | Dorman et al. | Jan 1987 | A |
4636526 | Dorman et al. | Jan 1987 | A |
4645503 | Lin et al. | Feb 1987 | A |
4654314 | Takagi et al. | Mar 1987 | A |
4661536 | Dorman et al. | Apr 1987 | A |
4672032 | Slavkin et al. | Jun 1987 | A |
4693986 | Vit et al. | Sep 1987 | A |
4713076 | Draenert | Dec 1987 | A |
4737411 | Graves, Jr. et al. | Apr 1988 | A |
4777153 | Sonuparlak et al. | Oct 1988 | A |
4781721 | Grundei | Nov 1988 | A |
4797282 | Wahlig et al. | Jan 1989 | A |
4798585 | Inoue et al. | Jan 1989 | A |
4839215 | Starling et al. | Jun 1989 | A |
4842603 | Draenert | Jun 1989 | A |
4846838 | Takai et al. | Jul 1989 | A |
4861733 | White | Aug 1989 | A |
4869906 | Dingeldein et al. | Sep 1989 | A |
4878914 | Miwa et al. | Nov 1989 | A |
RE33161 | Brown et al. | Feb 1990 | E |
4911720 | Collier | Mar 1990 | A |
RE33221 | Brown et al. | May 1990 | E |
4963145 | Takagi et al. | Oct 1990 | A |
4965039 | Schuetz | Oct 1990 | A |
5011495 | Hollinger | Apr 1991 | A |
5017518 | Hirayama et al. | May 1991 | A |
5030396 | Saita et al. | Jul 1991 | A |
5034352 | Vit et al. | Jul 1991 | A |
5053212 | Constantz et al. | Oct 1991 | A |
5055307 | Tsuru et al. | Oct 1991 | A |
5059388 | Kihara et al. | Oct 1991 | A |
5064436 | Ogiso et al. | Nov 1991 | A |
5089195 | Ichitsuka et al. | Feb 1992 | A |
5092888 | Iwamoto et al. | Mar 1992 | A |
5106626 | Parsons et al. | Apr 1992 | A |
5135394 | Hakamatsuka et al. | Aug 1992 | A |
5149368 | Liu et al. | Sep 1992 | A |
5152791 | Hakamatsuka et al. | Oct 1992 | A |
5171720 | Kawakami | Dec 1992 | A |
5207710 | Chu et al. | May 1993 | A |
5258029 | Chu et al. | Nov 1993 | A |
5263985 | Bao et al. | Nov 1993 | A |
5279831 | Constantz et al. | Jan 1994 | A |
5306303 | Lynch | Apr 1994 | A |
5318779 | Hakamatsuka et al. | Jun 1994 | A |
5338356 | Hirano et al. | Aug 1994 | A |
5338772 | Bauer et al. | Aug 1994 | A |
5385887 | Yim et al. | Jan 1995 | A |
5398483 | Smith et al. | Mar 1995 | A |
5409982 | Imura et al. | Apr 1995 | A |
5422340 | Ammann et al. | Jun 1995 | A |
5439951 | Glimcher et al. | Aug 1995 | A |
5464440 | Johansson | Nov 1995 | A |
5492697 | Boyan et al. | Feb 1996 | A |
5508267 | Czemuszka et al. | Apr 1996 | A |
5520923 | Tjia et al. | May 1996 | A |
RE35267 | Tsuru et al. | Jun 1996 | E |
5522893 | Chow et al. | Jun 1996 | A |
5522894 | Draenert | Jun 1996 | A |
5525148 | Chow et al. | Jun 1996 | A |
5531794 | Takagi et al. | Jul 1996 | A |
5536575 | Imura et al. | Jul 1996 | A |
5542973 | Chow et al. | Aug 1996 | A |
5545254 | Chow et al. | Aug 1996 | A |
5571185 | Schug | Nov 1996 | A |
5574075 | Draenert | Nov 1996 | A |
5597897 | Ron et al. | Jan 1997 | A |
5626861 | Laurencin et al. | May 1997 | A |
5641502 | Skalla et al. | Jun 1997 | A |
5650108 | Nies et al. | Jul 1997 | A |
5681872 | Erbe | Oct 1997 | A |
5695729 | Chow et al. | Dec 1997 | A |
5707962 | Chen et al. | Jan 1998 | A |
5717006 | Daculsi et al. | Feb 1998 | A |
5741329 | Agrawal et al. | Apr 1998 | A |
5766618 | Laurencin et al. | Jun 1998 | A |
5776193 | Kwan et al. | Jul 1998 | A |
5782971 | Constantz et al. | Jul 1998 | A |
5820632 | Constantz et al. | Oct 1998 | A |
5851670 | Mitoh et al. | Dec 1998 | A |
5866155 | Laurencin et al. | Feb 1999 | A |
5897953 | Ogawa et al. | Apr 1999 | A |
5914121 | Robey et al. | Jun 1999 | A |
5914356 | Erbe | Jun 1999 | A |
5922025 | Hubbard | Jul 1999 | A |
5939388 | Rosen et al. | Aug 1999 | A |
5962427 | Goldstein et al. | Oct 1999 | A |
5988171 | Sohn et al. | Nov 1999 | A |
6010648 | Yamamoto et al. | Jan 2000 | A |
6018095 | Lerch et al. | Jan 2000 | A |
6027744 | Vacanti et al. | Feb 2000 | A |
6030635 | Gertzman et al. | Feb 2000 | A |
6048964 | Lee et al. | Apr 2000 | A |
6051247 | Hench et al. | Apr 2000 | A |
6063117 | Perry | May 2000 | A |
6077989 | Kandel et al. | Jun 2000 | A |
6080801 | Draenert et al. | Jun 2000 | A |
6110503 | Rickey et al. | Aug 2000 | A |
6118043 | Nies et al. | Sep 2000 | A |
6129928 | Sarangapani et al. | Oct 2000 | A |
6136029 | Johnson et al. | Oct 2000 | A |
6165486 | Marra et al. | Dec 2000 | A |
6180605 | Chen et al. | Jan 2001 | B1 |
6180606 | Chen et al. | Jan 2001 | B1 |
6187046 | Yamamoto et al. | Feb 2001 | B1 |
6187047 | Kwan et al. | Feb 2001 | B1 |
6194006 | Lyons et al. | Feb 2001 | B1 |
6201065 | Pathak et al. | Mar 2001 | B1 |
6203574 | Kawamura | Mar 2001 | B1 |
6210612 | Pickrell et al. | Apr 2001 | B1 |
6235225 | Okada et al. | May 2001 | B1 |
6235665 | Pickrell et al. | May 2001 | B1 |
6261322 | Despres, III et al. | Jul 2001 | B1 |
6280478 | Richter et al. | Aug 2001 | B1 |
6281257 | Ma et al. | Aug 2001 | B1 |
6287341 | Lee et al. | Sep 2001 | B1 |
6296667 | Johnson et al. | Oct 2001 | B1 |
6306297 | Ichitsuka et al. | Oct 2001 | B1 |
6316091 | Richart et al. | Nov 2001 | B1 |
6325992 | Chow et al. | Dec 2001 | B1 |
6331312 | Lee et al. | Dec 2001 | B1 |
6335430 | Qvist | Jan 2002 | B1 |
6340648 | Imura et al. | Jan 2002 | B1 |
6346123 | McKay | Feb 2002 | B1 |
6350462 | Hakamatsuka et al. | Feb 2002 | B1 |
6358532 | Starling et al. | Mar 2002 | B2 |
6376573 | White et al. | Apr 2002 | B1 |
6395036 | Czemuszka et al. | May 2002 | B1 |
6432437 | Hubbard | Aug 2002 | B1 |
6432919 | Wang et al. | Aug 2002 | B1 |
6451059 | Janas et al. | Sep 2002 | B1 |
6458162 | Koblish et al. | Oct 2002 | B1 |
6479418 | Li et al. | Nov 2002 | B2 |
6495156 | Wenz et al. | Dec 2002 | B2 |
6511510 | de Bruijn et al. | Jan 2003 | B1 |
6521246 | Sapieszko et al. | Feb 2003 | B2 |
6524345 | Valimaa et al. | Feb 2003 | B1 |
6527810 | Johnson et al. | Mar 2003 | B2 |
6533821 | Lally | Mar 2003 | B1 |
6540784 | Barlow et al. | Apr 2003 | B2 |
6547866 | Edwards et al. | Apr 2003 | B1 |
6585992 | Pugh et al. | Jul 2003 | B2 |
6589590 | Czemuszka et al. | Jul 2003 | B2 |
6592787 | Pickrell et al. | Jul 2003 | B2 |
6599515 | Delmotte | Jul 2003 | B1 |
6616742 | Lin et al. | Sep 2003 | B2 |
6667049 | Janas et al. | Dec 2003 | B2 |
6670293 | Edwards et al. | Dec 2003 | B2 |
6692761 | Mahmood et al. | Feb 2004 | B2 |
6696073 | Boyce et al. | Feb 2004 | B2 |
6709744 | Day et al. | Mar 2004 | B1 |
6790233 | Brodke et al. | Sep 2004 | B2 |
6793725 | Chow et al. | Sep 2004 | B2 |
6800245 | Erbe et al. | Oct 2004 | B1 |
6808561 | Genge et al. | Oct 2004 | B2 |
6808585 | Boyce et al. | Oct 2004 | B2 |
6863899 | Koblish et al. | Mar 2005 | B2 |
6949251 | Dalal et al. | Sep 2005 | B2 |
6955716 | Xu et al. | Oct 2005 | B2 |
6987136 | Erbe et al. | Jan 2006 | B2 |
6987170 | Silverman et al. | Jan 2006 | B1 |
7018460 | Xu et al. | Mar 2006 | B2 |
7037304 | Lyles et al. | May 2006 | B2 |
7045125 | Erbe et al. | May 2006 | B2 |
7066999 | Lin et al. | Jun 2006 | B2 |
7081161 | Genge et al. | Jul 2006 | B2 |
7091260 | K hn | Aug 2006 | B2 |
7122057 | Beam et al. | Oct 2006 | B2 |
7172629 | McKay | Feb 2007 | B2 |
7238203 | Bagga et al. | Jul 2007 | B2 |
7258735 | Lin et al. | Aug 2007 | B2 |
7270705 | Lin et al. | Sep 2007 | B2 |
7294187 | Chow et al. | Nov 2007 | B2 |
7303646 | Qvist | Dec 2007 | B2 |
7318841 | Tofighi et al. | Jan 2008 | B2 |
7357941 | Dalal et al. | Apr 2008 | B2 |
7390498 | Dalal et al. | Jun 2008 | B2 |
7407542 | Lemaitre et al. | Aug 2008 | B2 |
7416602 | Murphy et al. | Aug 2008 | B2 |
7473312 | Barralet et al. | Jan 2009 | B2 |
7494950 | Armitage et al. | Feb 2009 | B2 |
7514248 | Gower et al. | Apr 2009 | B2 |
7514249 | Gower et al. | Apr 2009 | B2 |
7527687 | Genge et al. | May 2009 | B2 |
7544496 | Gower et al. | Jun 2009 | B2 |
7547449 | Gower et al. | Jun 2009 | B2 |
7575628 | Lu et al. | Aug 2009 | B2 |
7589133 | Pomrink | Sep 2009 | B2 |
7619016 | Dickens et al. | Nov 2009 | B2 |
7628851 | Armitage et al. | Dec 2009 | B2 |
7632878 | Xu et al. | Dec 2009 | B2 |
7648728 | Yamamoto et al. | Jan 2010 | B2 |
8173149 | Dalal et al. | May 2012 | B2 |
8232327 | Garigapati et al. | Jul 2012 | B2 |
8273803 | Garigapati et al. | Sep 2012 | B2 |
9265857 | Garigapati et al. | Feb 2016 | B2 |
20010014667 | Chen et al. | Aug 2001 | A1 |
20010051815 | Esplin | Dec 2001 | A1 |
20020022885 | Ochi | Feb 2002 | A1 |
20020035402 | de Bruijn et al. | Mar 2002 | A1 |
20020082694 | McKay | Jun 2002 | A1 |
20020114795 | Thorne et al. | Aug 2002 | A1 |
20020115742 | Trieu et al. | Aug 2002 | A1 |
20020151466 | Hubbard et al. | Oct 2002 | A1 |
20020193883 | Wironen | Dec 2002 | A1 |
20030009235 | Manrique et al. | Jan 2003 | A1 |
20030031698 | Roeder et al. | Feb 2003 | A1 |
20030069638 | Barlow et al. | Apr 2003 | A1 |
20030152606 | Gerber | Aug 2003 | A1 |
20040002770 | King et al. | Jan 2004 | A1 |
20040059416 | Murray | Mar 2004 | A1 |
20050217538 | Reinstort et al. | Oct 2005 | A1 |
20060089646 | Bonutti | Apr 2006 | A1 |
20060096504 | Grover et al. | May 2006 | A1 |
20060263443 | Chow et al. | Nov 2006 | A1 |
20070092580 | Chow et al. | Apr 2007 | A1 |
20070092856 | Chow et al. | Apr 2007 | A1 |
20070221093 | Erdrich et al. | Sep 2007 | A1 |
20080009955 | Shimp et al. | Jan 2008 | A1 |
20080028992 | Lee et al. | Feb 2008 | A1 |
20080069852 | Shimp et al. | Mar 2008 | A1 |
20080095817 | Murphy | Apr 2008 | A1 |
20080187571 | Clineff et al. | Aug 2008 | A1 |
20080188945 | Boyce et al. | Aug 2008 | A1 |
20090028949 | Leonard et al. | Jan 2009 | A1 |
20090028960 | Leonard et al. | Jan 2009 | A1 |
20090158964 | Insley et al. | Jun 2009 | A1 |
20090220475 | Bohner et al. | Sep 2009 | A1 |
20090258966 | Hirayarna et al. | Oct 2009 | A1 |
20100120923 | Stewart et al. | May 2010 | A1 |
20100129416 | Murphy et al. | May 2010 | A1 |
20100168798 | Clineff et al. | Jul 2010 | A1 |
20100305626 | Stewart et al. | Dec 2010 | A1 |
20110150963 | Clineff et al. | Jun 2011 | A1 |
20110151027 | Clineff et al. | Jun 2011 | A1 |
20110277931 | Garigapati et al. | Nov 2011 | A1 |
20110287067 | Stewart | Nov 2011 | A1 |
20120237568 | Murphy et al. | Sep 2012 | A1 |
Number | Date | Country |
---|---|---|
2620890 | Nov 1977 | DE |
4216496 | Nov 1993 | DE |
29922585 | Jul 2000 | DE |
10063119 | Aug 2001 | DE |
0267624 | May 1988 | EP |
0360244 | Mar 1990 | EP |
0987032 | Mar 2000 | EP |
1 027 897 | Aug 2000 | EP |
1142597 | Oct 2001 | EP |
2078696 | Jan 1982 | GB |
2 323 083 | Sep 1998 | GB |
2 348 872 | Oct 2000 | GB |
2354518 | Mar 2001 | GB |
59101145 | Jun 1984 | JP |
59131346 | Jul 1984 | JP |
59171546 | Sep 1984 | JP |
60142857 | Jul 1985 | JP |
6141466 | Feb 1986 | JP |
6145748 | Mar 1986 | JP |
6168054 | Apr 1986 | JP |
61127658 | Jun 1986 | JP |
63046167 | Feb 1988 | JP |
64-030572 | Feb 1989 | JP |
1108143 | Apr 1989 | JP |
1126977 | May 1989 | JP |
1230412 | Sep 1989 | JP |
3191963 | Aug 1991 | JP |
364482 | Oct 1991 | JP |
429630 | May 1992 | JP |
5042168 | Feb 1993 | JP |
526504 | Apr 1993 | JP |
5208877 | Aug 1993 | JP |
566909 | Sep 1993 | JP |
5237178 | Sep 1993 | JP |
5305134 | Nov 1993 | JP |
588687 | Dec 1993 | JP |
655219 | Jul 1994 | JP |
6296676 | Oct 1994 | JP |
6102582 | Dec 1994 | JP |
07-023994 | Jan 1995 | JP |
7291759 | Nov 1995 | JP |
9030988 | Feb 1997 | JP |
11276468 | Oct 1999 | JP |
2000262608 | Sep 2000 | JP |
2001058885 | Mar 2001 | JP |
3231135 | Sep 2001 | JP |
1986001113 | Feb 1986 | WO |
1987004110 | Jul 1987 | WO |
1987007495 | Dec 1987 | WO |
1993007835 | Apr 1993 | WO |
1993015721 | Aug 1993 | WO |
1993020859 | Oct 1993 | WO |
1994015652 | Jul 1994 | WO |
1994015653 | Jul 1994 | WO |
1995021634 | Aug 1995 | WO |
1997031661 | Sep 1997 | WO |
1997045147 | Dec 1997 | WO |
1997046178 | Dec 1997 | WO |
1998038949 | Sep 1998 | WO |
1998047485 | Oct 1998 | WO |
1999016478 | Apr 1999 | WO |
1999016479 | Apr 1999 | WO |
1999020319 | Apr 1999 | WO |
1999037246 | Jul 1999 | WO |
00018443 | Apr 2000 | WO |
00035511 | Jun 2000 | WO |
00045870 | Aug 2000 | WO |
00045871 | Aug 2000 | WO |
00050104 | Aug 2000 | WO |
01003709 | Jan 2001 | WO |
01013970 | Mar 2001 | WO |
01028603 | Apr 2001 | WO |
01029189 | Apr 2001 | WO |
01044141 | Jun 2001 | WO |
01054746 | Aug 2001 | WO |
01066163 | Sep 2001 | WO |
0211781 | Feb 2002 | WO |
0215881 | Feb 2002 | WO |
2006019649 | Feb 2006 | WO |
2010056811 | May 2010 | WO |
2011143226 | Nov 2011 | WO |
Entry |
---|
(Vo-Dinh) Nanotechnology in Biology and Medicine, CRC Press (Jan. 24, 2007) [retrieved on Feb. 8, 2012] entire document retrieved from the Internet: <URL:http://books.google.com/books/aboutNanotechnology-in-biology-and-medicine.html?id=t9IPt1Hu6bAC>, (1 page). |
A. Reinstorf et al., “Phosphoserine—a convenient compound for modification of calcium phosphate bone cement collagen composites,” Journal of Materials Science: Materials in Medicine 15 (2004), pp. 451-455, 2004 Kluwer Academic Publishers, Dresden, Germany. |
Andriano, K.P. et al., “Preliminary In Vivo Studies on the Osteogenic Potential of Bone Morphogenetic Proteins Delivered from an Absorbable Puttylike Polymer Matrix,” J. Biomed. Mater Re (Appl. Biomater.) 53:36-43 (2000). (8 pages). |
Benoit, M.-A. et al., “Antibiotic-Loaded Plaster of Paris Implants Coated With Poly Lactide-co-glycolide as a Controlled Release Delivery System for the Treatment of Bone Infections,” International Orthopaedics 21(6):403-408 (1997). (6 pages). |
Breitbart, A.S. et al., “Tricalcium Phosphate and Osteogenin: A Bioactive Onlay Bone Graft Substitute,” Plastic and Reconstructive Surgery 96:699-708 (1995). (10 pages). |
Bucholz, R.W. et al., “Hydroxyapatite and Tricalcium Phosphate Bone Graft Substitutes,” Orthopedic Clinics of North America 18:323-334 (1987). (12 pages). |
Bundy et al. “The Effect of Surface Preparation on Metal/Bone Cement Interfacial Strength” Journal of Biomedical Materials Research, vol. 21, Issue 6, pp. 773-805 esp. abstract, Jun. 1987. |
Chow et al. “A Natural Bone Cement—A Laboratory Novelty Led to the Development of Revolutionary New Biomaterials” Journal of Research of the National Institute of Standards and Technology, vol. 106, No. 6, pp. 1029-1033, Nov. 2001. |
Chow, L.C., “Solubility of Calcium Phosphates,” Monogr Oral Sci. Basel. Karger 18:94-111 (2001). (18 pages). |
Clarke “Role of Ceramic Implants, Design and Clinical Success With Total Hip Prosthetic Ceramic-to-Ceramic Bearings” Clinical Orthopaedics and Related Research, vol. 282, pp. 19-30 esp. abstract, Sep. 1992. |
Eggli, P.S. et al., “Porous Hydroxyapatite and Tricalcium Phosphate Cylinders with Two Different Pore Size Ranges Implanted in the Cancellous Bone of Rabbits,” Clinical Orthopaedics and Related Research 232:127-138 (1988). (12 pages). |
Fujimura et al. “A bioactive bone cement containing Bis-GMA resin and A-W glass-ceramic as an augmentation graft material on mandibular bone” Clinical Oral Implants Research, vol. 14, Issue 5, pp. 659-667 esp. abstract, Oct. 2003. |
Gerhart, T.N. et al., “In vitro Characterization and Biomechanical Optimization of a Biodegradable Particulate Composite Bone Cement,” Journal of Biomedical Materials Research 22:1071-1082 (1988). (12 pages). |
Gombotz, W.R. et al., “Stimulation of Bone Healing by Transforming Growth Factor-Beta1 Released from Polymeric or Ceramic Implants,” Journal of Applied Biomaterials 5(2):141-150 (1994). (10 pages). |
Hua, W. et al. “New Bone Formation in the in vivo Implantation of Bioceramics,” Chinese Medical Journal, vol. 105, pp. 753-757 (1992) (5 pages). |
Hutmacher, D.W., “Scaffolds in Tissue Engineering Bone and Cartilage,” Biomaterials 21:2529-2543 (2000). (14 pages). |
International Preliminary Report on Patentability for Application No. PCT/US2009/64135 dated May 26, 2011. |
International Search Report and Written Opinion, dated Feb. 21, 2012, International Application No. PCT/US2011/060956, Applicant Howmedica Osteonics Corp. (13 pages). |
International Search Report for Application No. PCT/US2009/64135 dated Apr. 2, 2010. |
Ishikawa et al. “Behavior of a Calcium Phosphate Cement in Simulated Blood Plasma in Vitro” Dental Materials, vol. 10, Issue 1, pp. 26-32 esp. abstract, Jan. 1994. |
Laffargue, PH. et at., “Evaluation of Human Recombinant Bone Morphogenetic Protein-2-Loaded Tricalcium Phosphate Implants in Rabbits' Bone Defects,” Bone 25:55S-58S (1999). (4 pages). |
Lange, T.A. et al. “Granular Tricalcium Phosphate in Large Cancellous Defects,” Annals of Clinical and Laboratory Science, vol. 16, No. 6, pp. 467-472 (1986) (6 pages). |
Mai et al. “O-phospho-L-serine: A Modulator of Bone Healing in Calciumphosphate Cements” Biomedizinische Technik, vol. 53, Issue 5, p. 229-233 esp. abstract, Oct. 2008. |
Metsger, D.S. et al., “Histomorphometric Analysis of Tricalcium Phosphate Ceramic Implanted Into Turkeys,” Bone 14:243-248 (1993). (6 pages). |
Nade, S. et at., “Osteogenesis After Bone and Bone Marrow Transplantation,” Clinical Orthopaedics and Related Research 181: 255-263 (1983). (9 pages). |
Nery, E.B. et al., “Tissue Response to Biphasic Calcium Phosphate Ceramic With Different Ratios of HA/betaTCP in Periodontal Osseous Defects,” Journal of Periodontology 63:729-735 (1992). (7 pages). |
Ohgushi, H. et al., “Marrow Cell Induced Osteogenesis in Porous Hydroxyapatite and Tricalcium Phosphate: A Comparative Histomorphometric Study of Ectopic Bone Formation,” Journal of Biomedical Materials Research 24:1563-1570 (1990). (8 pages). |
Ohura, K. et at., “Healing of Segmental Bone Defects in Rats Induced by a beta-TCP-MCPM Cement Combined with rhBMP-2,” Journal of Biomedical Materials Research 44:168-175 (1999). (7 pages). |
Soriano, I. et at., “Formulation of Calcium Phosphates/Poly (d,l-lactide) Blends Containing Gentamicin for Bone Implantation,” Journal of Controlled Release 68:121-134 (2000). (14 pages). |
Tancred, D.C. et at., “A Synthetic Bone Implant Macroscopically Identical to Cancellous Bone,” Biomaterials 19:2303-2311 (1998). (9 pages). |
Thoma, K. et al., “Biodegradable Controlled Release Implants Based on beta-Tricalcium Phosphate Ceramic,” European Journal Pharmaceutics and Biopharmaceutics 38:107-112 (1992). (6 pages). |
Uchida, A. et al., “The Use of Ceramics for Bone Replacement,” The Journal of Bone and Joint Surgery 66-B:269-275 (1984). (7 pages). |
Uchida, A. et at., “Bone Ingrowth into Three Different Porous Ceramics Implanted into the Tibia of Rats and Rabbits,” Journal of Orthopaedic Research 3:65-77 (1985). (13 pages). |
Vaccaro, A.R., “The Role of the Osteoconductive Scaffold in Synthetic Bone Graft,” Orthopedics 25(5):571-578 (2002). (8 pages). |
W. Schneiders et al., “Effect of modification of hydroxyapatite-collagen composites with sodium citrate, phosphoserine, phosphoserine-RGD-peptide and calcium carbonate on bone remodelling,” Bone 40 (2007) pp. 1048-1059, Elsevier, Nov. 19, 2006, Dresden, Germany, ww.elsevier.com-locate-bone. |
White, E. et al., “Biomaterial Aspects of Interpore-200 Porous Hydroxyapatite,” Dental Clinics of North America 30 (1):49-67 (1986). (19 pages). |
Zheng, Q. et al. “Artificial bone of Porous Tricalcium Phosphate Ceramics and Its Preliminary Clinical Application,” Journal of Tongji Medical University, vol. 12, pp. 173-178 (1992) (6 pages). |
Frayssinet, P. and Rouquet, N. “High Compressive Strength Macroporous Calcium Phosphate Ceramics for Bone Repair,” Journal of Biomechanics (1998) 31 (Supp1): 50-50. |
Number | Date | Country | |
---|---|---|---|
20180250432 A1 | Sep 2018 | US |
Number | Date | Country | |
---|---|---|---|
61333478 | May 2010 | US |
Number | Date | Country | |
---|---|---|---|
Parent | 14980022 | Dec 2015 | US |
Child | 15873381 | US |
Number | Date | Country | |
---|---|---|---|
Parent | 13104716 | May 2011 | US |
Child | 14980022 | US |