Patent Application
20180193417
The present invention relates to methods of treating and preventing acute Graft versus host disease (GVHD).
All documents mentioned in the text and listed at the end of this description are incorporated herein by reference.
Graft versus host disease (GVHD) is believed to arise from a complex interaction between donor and recipient adaptive immunity. GVHD often occurs in humans after allogeneic stem-cell transplantation, but may also arise after transplantation of solid organs containing lymphoid tissue and transfusion of unirradiated blood products. Acute GVHD describes a distinctive syndrome of dermatitis, hepatitis, and enteritis developing within 100 days of treatment e.g. allogeneic hematopoietic-cell transplantation (HCT). Chronic GVHD describes a more diverse syndrome developing after day 100.
For GVHD to occur the following criteria must be met, discussed in [1]:
Patient groups at risk for GVHD include allogenic HCT patients, such as those that are receiving no GVHD prophylaxis, older patients, recipients of HLA-non-identical stem cells and recipients of grafts. Solid-organ transplantation patients at risk for GVHD include recipients of small-bowel transplants (organs containing lymphoid tissue). Patients who are subject to transfusion of unirradiated blood products at particular risk include those that have congenital immunodeficiency syndromes, neonates and foetuses, those that are receiving immunosuppressive chemoradiotherapy and those that are receiving directed blood donations from partially HLA-identical, HLA-homologous donors
Autologous GVHD may also occur. This occurs after autologous or syngeneic HCT and is believed to arise from tissue damage caused by treatment or by secondary cytokine production, which exposes cryptic self-antigens, which the immune system may newly recognize only after HCT. Transfusion-associated GVHD occurs 4-30 days after transfusion and resembles hyperacute GVHD after allogeneic HCT.
Acute GVHD may initially appear within 100 days of transplant. Hyperacute GVHD is GVHD that arises 7-14 days after transplantation. Acute GVHD may have involvement of the skin (a pruritic or painful rash), liver (e.g. asymptomatic elevation of bilirubin, alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase levels similar to those observed with cholestatic jaundice, and ensuing pruritus with hyperbilirubinemia), or the intestine (e.g. the distal small bowel and colon, e.g. resulting in diarrhea, intestinal bleeding, cramping abdominal pain, and ileus, or the upper-GI, manifesting as anorexia and dyspepsia without diarrhea). Acute GVHD also has been associated with increased risk of infectious and noninfectious pneumonia and sterile effusions, hemorrhagic cystitis with infective agents, thrombocytopenia, and anemia. Hemolytic-uremic syndrome (thrombotic microangiopathy) has been observed in patients given cyclosporine A (CSP A) who developed severe GVHD.
Chronic GVHD is viewed as an extension of acute GVHD. However, it also may occur for the first time in patients who never have clinical evidence of acute GVHD, or it may emerge after a quiescent interval after acute GVHD resolves.
Various systems exist for staging and grading GVHD (see [2]):
Existing treatments for acute GVHD include prophylaxis and treatment as set out in Table 3 below:
Co-pending PCT application PCT/EP2015/062742 describes and claims methods of treating complement mediated disorders in patients who are resistant to eculizumab, and [3] also presents preliminary experimental work showing that when serum from an eculizumab resistant patient was spiked with Coversin and eculizumab, the dose response curve was normal for Coversin but not for eculizumab.
There is a need for treatments that improve on, or are at least alternatives to the known treatments for GVHD.
The present inventors have found that in a patient suffering from GVHD, who had previously not responded to treatment with eculizumab, a C5 monoclonal antibody, the systemic administration of the tick protein Coversin (also referred to as EV576 and OmCI in the art and herein [19]) led to stabilisation of vital signs and reduced rectal bleeding. Systemically administered coversin can therefore be used to treat and prevent acute GVHD.
The invention provides a method of treating or preventing acute GVHD comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
The invention also provides an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
The invention also provides a method of treating or preventing acute GVHD comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a nucleic acid molecule encoding a protein comprising amino acids 19 to 168 of the amino acid sequence in
The invention also provides an agent which is a nucleic acid molecule encoding a protein comprising amino acids 19 to 168 of the amino acid sequence in
The complement system is an essential part of the body's natural defense mechanism against foreign invasion and is also involved in the inflammatory process. More than 30 proteins in serum and at the cell surface are involved in complement system function and regulation. Recently it has become apparent that, as well as the ˜35 known components of the complement system which may be associated with both beneficial and pathological processes, the complement system itself interacts with at least 85 biological pathways with functions as diverse as angiogenesis, platelet activation, glucose metabolism and spermatogenesis
The complement system is activated by the presence of foreign antigens. Three activation pathways exist: (1) the classical pathway which is activated by IgM and IgG complexes or by recognition of carbohydrates; (2) the alternative pathway which is activated by non-self surfaces (lacking specific regulatory molecules) and by bacterial endotoxins; and (3) the lectin pathway which is activated by binding of mannan-binding lectin (MBL) to mannose residues on the surface of a pathogen. The three pathways comprise parallel cascades of events that result in the production of complement activation through the formation of similar C3 and C5 convertases on cell surfaces resulting in the release of acute mediators of inflammation (C3a and C5a) and formation of the membrane attack complex (MAC). The parallel cascades involved in the classical and alternative pathways are shown in
The classical complement pathway, the alternative complement pathway and the lectin complement pathway are herein collectively referred to as the complement pathways.
Several polymorphisms of human C5 have been reported [4-8]. Mutations in the gene encoding C5 have been associated with various pathologies including complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis. Polymorphisms in human C5 include insertions, deletions, single amino acid substitutions, frame-shifts, truncations and combinations of these changes.
Certain polymorphisms of human C5 alter C5 activity with clinical significance, e.g. polymorphisms affecting Arg885 of wildtype C5 include Arg885Cys (encoded by c.2653C>T) and p.Arg885His (encoded by c.2654G>A), both of which decrease the theraputic effectiveness of the mAb eculizumab [7].
The term “C5 polymorphism” is used herein to mean any variant of C5 other than the wild-type C5. In a human subject, the wild-type C5 is the C5 protein with accession number NP_001726.2; version GI:38016947. The term “C5 polymorphism” includes insertions, deletions, single or multiple amino acid substitutions, frame-shifts, truncations and combinations of these changes in the C5 protein.
These polymorphisms can be present as either heterozygous or homozygous polymorphisms, such as heterozygous C5 for a given polymorphism, homozygous for one polymorphism or heterozygous for different polymorphisms.
Polymorphisms include changes to the amino acid sequence of wildtype C5 which are in proximity to, or within the epitope for eculizumab, (i.e. 879KSSKC883, including K879, S880, S881, K882 and/or C883). For example, any change may be in the epitope for eculizumab or up to 10, 9, 8, 7, 6, 5, 4, 3, 2, 1 amino acids to the N- or C-terminus of the epitope for eculizumab.
Preferably, the amino acid change is not within or in proximity to the Coversin binding site of C5. This is believed to be a conserved region atop C5a at the distal end of the highly conserved CUB-C5d-MG8 superdomain of C5.
C5 polymorphisms may decrease the effectiveness of one or more agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with wild-type C5. By “decrease the effectiveness” it is meant that the agent has an IC50 for the polymorphic C5 protein that is at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 100, 1000 or more times greater than the IC50 of the same agent for the wild-type C5 protein.
The C5 polymorphism may decrease the effectiveness of one or more agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway, but does not decrease the effectiveness of Coversin or functional equivalents thereof. The C5 polymorphism may decrease the effectiveness of one or more anti-C5 monoclonal antibodies that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway in a subject with wild-type C5, but does not decrease the effectiveness of other agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by binding to C5 without blocking the C5 convertase binding site.
By “does not decrease the effectiveness” it is meant that the IC50 of Coversin or other agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by binding to C5 without blocking the C5 convertase binding site, for the wild-type C5 protein is at least 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or 100% of the IC50 of Coversin or other agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by binding to C5 without blocking the C5 convertase binding site for the polymorphic C5 protein. The term “does not decrease” also encompasses an increase in effectiveness.
In an alternative embodiment, effectiveness can be measured by measuring the ability of the agent to inhibit complement activation in serum taken from the subject. For example, complement activity in the serum of said subjects can be measured by any means known in the art or described herein, for example the haemolytic assays described in reference [9]. An agent would be considered to inhibit complement activity in said subject if complement activity in the presence of the agent is reduced when compared to a control. By “reduced” in this context it is meant that complement activity in the treated sample is at least 10, 20, 30, 40, 50, 60, 70, 80, 90, or 100%, reduced compared to a control.
The C5 polymorphism may decrease the effectiveness of monoclonal antibody agents in inhibiting activation of one or more of the complement pathways. The C5 polymorphism may decrease the effectiveness of the monoclonal antibody eculizumab in inhibiting activation of one or more of the complement pathways. The C5 polymorphism may decrease the effectiveness of agents that inhibit the classical complement pathway, the alternative complement pathway and the lectin complement pathway by blocking the C5 convertase binding site. The C5 polymorphism may be at position Arg885. Specific polymorphisms at this position include Arg885Cys or Arg885His.
Polymorphisms that alter binding the affinity of C5 to known anti-C5 monoclonal antibodies such as eculizumab, Pexelizumab, and/or N19-8, or the effectiveness of peptidic complement inhibitors such as ARC1905 are also of interest.
Thus, in a specific embodiment the invention provides a method of treating or preventing acute GVHD comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
Specific examples of such polymorphisms and anti C5 antibodies are discussed above.
The invention also provides an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
Specific examples of such polymorphisms and anti C5 antibodies are discussed above.
The invention also provides a method of treating or preventing acute GVHD comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a nucleic acid molecule encoding a protein comprising amino acids 19 to 168 of the amino acid sequence in
Specific examples of such polymorphisms and anti C5 antibodies are discussed above.
The invention also provides an agent which is a nucleic acid molecule encoding a protein comprising amino acids 19 to 168 of the amino acid sequence in
Specific examples of such polymorphisms and anti C5 antibodies are discussed above.
As discussed above, subjects with two C5 polymorphisms at position Arg885 (c.2653C>T (p.Arg885Cys) and c.2654G>A (p.Arg885His)) do not respond to eculizumab, and such subjects are examples of subjects who are eculizumab resistant. Coversin has been shown to be able to inhibit C5 cleavage and activation of the complement pathways irrespective of the presence or absence of the polymorphisms because it interacts with complement C5 protein in a different manner to eculizumab. Coversin and eculizumab bind to C5 at different locations on the C5 molecule. Coversin binds to C5, which results in stabilization of the global conformation of C5 but does not block the C5a cleavage site [10].
The polymorphism Arg885His is particularly prevalent in subjects of Japanese and Han Chinese origin. Coversin can be used in a sub-population with these ethnic origins. Coversin may also be used in populations of other ethnic origins, e.g. those who are not of Japanese origin or Han Chinese origin and/or those who are not Caucasian. Suitable ethnic groups include the Afro-Caribbean, Slavic Caucasian, Non-Slavic Caucasian, Oriental Asiatic and Non Oriental Asiatic groups.
Subjects with C5 polymorphisms can be identified by routine techniques including molecular genetic analysis of the gene encoding the C5 protein including sequencing of the gene [7]; testing the ability of various agent to inhibit complement activation in the subject as described herein or by other methods known in the art; and/or biochemical analysis of the C5 protein from the subject, including isoelectric focusing and functional detection [11]. In a clinical setting, a subject with a C5 polymorphism may be identified by an unexpectedly poor response to an agent that inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway.
Complement activity in the serum of said subjects can be measured by any means known in the art or described herein, for example the haemolytic assays described in reference [12] and/or by using the Quidel CH50 method as referred to in the example. An agent would be considered to inhibit complement activity in said subject if complement activity in the presence of the agent is reduced when compared to a control. By “reduced” in this context it is meant that complement activity in the treated sample is at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 100%, reduced compared to a control.
Activation of complement must be tightly controlled to prevent damage to the body's own tissues. Failure to control complement activation has been shown to play a role in a variety of diseases including, amongst others, acute pancreatitis, Age Related Macular Degeneration (AMD), atypical haemolytic uremeic syndrome (aHUS), Alzheimer's disease, Huntingdon's disease, Parkinson's disease, allergic encephalomyelitis, allotransplatation, asthma, adult respiratory distress syndrome, influenza, burn injuries, Crohn's disease, glomerulonephritis, haemolytic anaemia, haemodialysis, hereditary angioedema, ischaemia reperfusion injuries, multiple system organ failure, multiple sclerosis, myasthenia gravis, myocardial infarction, paroxysmal nocturnal haemoglobinuria (PNH), psoriasis, rheumatoid arthritis, septic shock, systemic lupus erythematosus, stroke, thrombotic thrombocytopaenicpurpura (TTP), traumatic brain injury, vascular leak syndrome, and transplantation rejection and graft versus host disease (GvHD), as well as various other peripheral nerve disorders and respiratory disorders [13-18].
The invention concerns the treatment of acute GVHD.
The subject may be suffering from acute GVHD, may have GVHD with one or more symptoms that are at stage +, ++, +++ or ++++ and/or the subject may have a clinical grading of I, II, III or IV [2]. The outcome of the treatment of the invention may be an improvement in the stage and/or grade of the GVHD.
The subject suffering from acute GVHD may have tissue damage, e.g. internal (such as intestinal) tissue damage arising from the GVHD. As such, the outcome of the treatment of the invention may be a reduction in this tissue damage.
Symptoms of GVHD can be measured by serum LDH. As such the outcome of the treatment of the invention may be a reduction in serum LDH e.g. as measured by standard methods known in the art.
The subject may have a reduced platelet count. As such the outcome of the treatment of the invention may be an increase in platelet count, e.g. as measured by standard methods known in the art.
Thus in a further embodiment of the invention, there is provided a method of reducing tissue damage in a subject with acute GVHD, comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
Thus in a further embodiment of the invention, there is provided a method of reducing serum LDH in a subject with acute GVHD, comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
Thus in a further embodiment of the invention, there is provided a method of increasing platelet count in a subject with acute GVHD, comprising systemically administering to a subject in need thereof a therapeutically or prophylactically effective amount of an agent which is a protein comprising amino acids 19 to 168 of the amino acid sequence in
Preferred subjects, agents, doses and the like are as disclosed herein. Any reference to any reduction or increase is a reduction or increase compared to said subject in the absence of the treatment. Preferably, the parameter can be quantitated and where this is the case the increase or decrease is preferably statistically significant. For example the increase or decrease may be at least 3, 5, 10, 15, 20, 30, 40, 50% compared to the parameter in the absence of treatment.
The subject to which the agent is administered in the practice of the invention is preferably a mammal, preferably a human. The subject may be an adult, a child, or an infant. The subject to which the agent is administered is suffering from acute GVHD.
The subject may be e.g. at least 5 years old, or at least 6, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 50, 60, 70 years old. The subject may be less than 4 years old, less than 3, 2, or 1 year old.
Subjects of interest are those with acute GVHD, as described herein. Preferred subjects may, for example have one or more of the following attributes:
According to one embodiment of the invention, the agent is a protein comprising amino acids 19 to 168 of the amino acid sequence in
According to an alternative embodiment, the protein used according to this embodiment of the invention may comprise or consist of amino acids 1 to 168 of the amino acid sequence in
The protein having the amino acid sequence given in
The Coversin protein and the ability of this protein to inhibit complement activation has been disclosed in [19], where the Coversin protein was referred to as the “OmCI protein”. The Coversin protein has also been shown to be effective in the treatment of myasthenia gravis [15], respiratory disorders [16] and peripheral nerve disorders [17]. The ability of the Coversin protein to bind eicosanoids including LTB4 and its use in the treatment of diseases mediated by a leukotriene or hydroxyeicosanoid has been suggested in [20].
It has now been found that the Coversin protein is effective in the treatment and prevention of acute GVHD in subjects. The data presented herein demonstrate that, in a subject with GVHD, complement activity in vitro was completely sensitive to Coversin, with 100% inhibition at all concentrations tested, and the subject furthermore showed an encouraging clinical response, with stabilisation of vital signs and reduced rectal bleeding.
According to a further embodiment of the invention, the agent may be a nucleic acid molecule encoding the Coversin protein or a functional equivalent thereof. For example, gene therapy may be employed to effect the endogenous production of the Coversin protein by the relevant cells in the subject, either in vivo or ex vivo. Another approach is the administration of “naked DNA” in which the therapeutic gene is directly injected into the bloodstream or into muscle tissue.
Preferably, such a nucleic acid molecule comprises or consists of bases 55 to 507 of the nucleotide sequence in
The Coversin protein has been demonstrated to bind to C5 and prevent its cleavage by C5 convertase in rat, mouse and human serum with an IC50 of approximately 0.02 mg/ml. Functional equivalents of the Coversin protein preferably retain the ability to bind C5 with an IC50 of less than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than 0.05 mg/ml, preferably less than 0.02 mg/ml, preferably less than preferably less than 100 ng/ml, preferably less than 10 ng/ml, more preferably still, less than 1 ng/ml.
The Coversin protein has also been demonstrated to bind LTB4. Functional equivalents of the Coversin protein may also retain the ability to bind LTB4 with a similar affinity as the Coversin protein.
In one aspect of the invention, the agent may bind complement C5, including complement C5 from subjects with complement C5 polymorphisms. The agent may act to prevent the cleavage of complement C5, including complement C5 from subjects with complement C5 polymorphisms, by C5 convertase into complement C5a and complement C5b-9. The agent may act to reduce C5a levels in a subject compared to an untreated subject. Functional equivalents of the agent also preferably share these properties.
In one aspect of the invention, the agent inhibits the classical complement pathway, the alternative complement pathway and the lectin complement pathway. In one aspect the agent binds to C5 in such a way as to stabilize the global conformation of C5 but not block the C5 convertase cleavage site. Binding of Coversin to C5 results in stabilization of the global conformation of C5 but does not block the convertase cleavage site. Functional equivalents of the agent also preferably share these properties.
The complement C5 protein, also referred to herein as C5, is cleaved by the C5 convertase enzyme (
MAC is associated with other important pathological processes including rheumatoid arthritis [22;23], proliferative glomerulonephritis [24], idiopathic membranous nephropathy [25], proteinurea [26], demyelination after acute axonal injury [27] and is also responsible for acute graft rejection following xenotransplantation [28].
Monoclonal antibodies and small molecules that bind and inhibit C5 have been developed to treat various diseases [14], in particular PNH, psoriasis, rheumatoid arthritis, systemic lupus erythematosus and transplant rejection. However, these monoclonal antibodies do not bind to certain C5 proteins from subjects with C5 polymorphisms, and are thus ineffective in these subjects [7]. In contrast, the Coversin agents, and functional equivalents thereof, inhibit complement C5 cleavage both in subjects with wild-type C5 and in subjects with C5 polymorphisms.
The ability of an agent to bind C5, including C5 from subjects with C5 polymorphisms, may be determined by standard in vitro assays known in the art, for example by western blotting following incubation of the protein on the gel with labelled C5. Preferably, the agent according to the invention binds C5, either wild-type and/or C5 from subjects with C5 polymorphisms, with an IC50 of less than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than 0.05 mg/ml, preferably less than 0.04 mg/ml, preferably less than 0.03 mg/ml, preferably 0.02 mg/ml, preferably less than 1 μg/ml, preferably less than 100 ng/ml, preferably less than 10 ng/ml, more preferably still, less than 1 ng/ml. The agent need not have the same affinity for wild-type C5 and C5 from subjects with C5 polymorphisms. It may show higher, lower or the same affinity for wild-type C5 and C5 from subjects with C5 polymorphisms.
The ability of an agent to inhibit complement activation may be determined by measuring the ability of the agent to inhibit complement activation in serum. For example, complement activity in the serum can be measured by any means known in the art or described herein.
The agent may also be defined as having the function of inhibiting eicosanoid activity.
The agent according to this aspect of the invention may inhibit leukotrine B4 (LTB4) activity. In particular, the agent according to this aspect of the invention may bind LTB4. The ability of an agent to bind LTB4 may be determined by standard in vitro assays known in the art, for example by means of a competitive ELISA between coversin and anti-LTB4 antibody competing for binding to labelled LTB4. The agent according to the invention may bind LTB4 with an IC50 of less than 0.2 mg/ml, preferably less than 0.1 mg/ml, preferably less than 0.05 mg/ml, preferably less than 0.04 mg/ml, preferably less than 0.03 mg/ml, preferably 0.02 mg/ml, preferably less than 1 μg/ml, preferably less than 100 ng/ml, preferably less than 10 ng/ml, more preferably still, less than 1 ng/ml. Functional equivalents of the agent also preferably share these properties.
According to one embodiment of the invention, the agent may bind all of C5, C5 from subjects with C5 polymorphisms, and LTB4. The agent according to this embodiment may thus act to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9 (MAC), and also to inhibit LTB4 activity. Using an agent which binds to both C5 and LTB4 is particularly advantageous. C5 and the eicosanoid pathway are both believed to contribute to the observed pathology in many complement mediated diseases and disorders. Thus by using a single agent which inhibits multiple pathways involved in the inflammatory effects of complement-mediated diseases and disorders, an enhanced effect can be achieved, compared to using an agent which inhibits only a single pathway involved in the inflammatory effects of complement-mediated diseases and disorders. There are furthermore practical advantages associated with administering a single molecule.
Preferably, the agent of the invention is derived from a haematophagous arthropod. The term “haematophagous arthropod” includes all arthropods that take a blood meal from a suitable host, such as insects, ticks, lice, fleas and mites. Preferably, the agent is derived from a tick, preferably from the tick Ornithodoros moubata.
In one respect, the term “functional equivalent” is used herein to describe homologues and fragments of the Coversin protein which: a) retain its ability to bind C5, either wild-type C5 or C5 from a subject with a C5 polymorphism, and to prevent the cleavage of complement C5 by C5 convertase into complement C5a and complement C5b-9; and/or b) retain its ability to bind LTB4.
The term “functional equivalent” also refers to molecules that are structurally similar to the Coversin protein or that contain similar or identical tertiary structure, particularly in the environment of the active site or active sites of the Coversin protein that binds to C5, either wild-type C5 or C5 from a subject with a C5 polymorphism, and/or LTB4, such as synthetic molecules. Amino acids in Coversin that are likely to be required for LTB4 binding are described in [20].
The term “homologue” is meant to include reference to paralogues and orthologues of the Coversin sequence that is explicitly identified in
Methods for the identification of homologues of the Coversin sequence given in
Typically, greater than 30% identity between two polypeptides (preferably, over a specified region such as the active site) is considered to be an indication of functional equivalence and thus an indication that two proteins are homologous. Preferably, proteins that are homologues have a degree of sequence identity with the Coversin protein sequence identified in
Functional equivalents of the Coversin protein sequence given in
Fragments of the Coversin protein and of homologues of the Coversin protein are also embraced by the term “functional equivalents” providing that such fragments retain the ability to bind wild-type C5 and/or C5 from subjects with a C5 polymorphism and/or LTB4. Fragments may include, for example, polypeptides derived from the Coversin protein sequence which are less than 150 amino acids, less than 125 amino acids, less than 100 amino acids, less than 75 amino acids, less than 50 amino acids, or even 25 amino acids or less, provided that these fragments retain the ability to bind to complement wild-type C5 and/or C5 from subjects with a C5 polymorphism and/or LTB4. Fragments may include, for example, polypeptides derived from the Coversin protein sequence which are at least 150 amino acids, at least 125 amino acids, at least 100 amino acids, at least 75 amino acids, at least 50 amino acids, or at least 25 amino acids, provided that these fragments retain the ability to bind to complement wild-type C5 and/or C5 from subjects with a C5 polymorphism and/or LTB4.
Any functional equivalent or fragment thereof preferably retains the pattern of cysteine residues that is found in Coversin. For example, said functional equivalent comprises six cysteine residues that are spaced relative to each other at a distance of 32 amino acids apart, 62 amino acids apart, 28 amino acids apart, 1 amino acid apart and 21 amino acids apart as arranged from the amino terminus to the carboxyl terminus of the sequence according to amino acids 1 to 168 of the amino acid sequence in
Included as such fragments are not only fragments of the O. moubata Coversin protein that is explicitly identified herein in
A functional equivalent used according to the invention may be a fusion protein, obtained, for example, by cloning a polynucleotide encoding the Coversin protein in frame to the coding sequences for a heterologous protein sequence. The term “heterologous”, when used herein, is intended to designate any polypeptide other than the Coversin protein or its functional equivalent. Example of heterologous sequences, that can be comprised in the soluble fusion proteins either at N- or at C-terminus, are the following: extracellular domains of membrane-bound protein, immunoglobulin constant regions (Fc region), multimerization domains, domains of extracellular proteins, signal sequences, export sequences, or sequences allowing purification by affinity chromatography. Many of these heterologous sequences are commercially available in expression plasmids since these sequences are commonly included in the fusion proteins in order to provide additional properties without significantly impairing the specific biological activity of the protein fused to them [29]. Examples of such additional properties are a longer lasting half-life in body fluids, the extracellular localization, or an easier purification procedure as allowed by a tag such as a histidine, GST, FLAG, avidin or HA tag.
The Coversin protein and functional equivalents thereof, may be prepared in recombinant form by expression in a host cell. Such expression methods are well known to those of skill in the art and are described in detail by [30] and [31]. Recombinant forms of the Coversin protein and functional equivalents thereof are preferably unglycosylated.
The proteins and fragments of the present invention can also be prepared using conventional techniques of protein chemistry. For example, protein fragments may be prepared by chemical synthesis. Methods for the generation of fusion proteins are standard in the art and will be known to the skilled reader. For example, most general molecular biology, microbiology recombinant DNA technology and immunological techniques can be found in [30] or [32].
Coversin and its functional equivalents do not require a medical professional for administration to be carried out, and these molecules are rapidly absorbed. Many recombinant antibodies are absorbed very slowly and as a result need to be infused over long periods (e.g. intravenously). The administration of such molecules thus also requires a medical professional. Thus, as well as having the advantage of being more effective at inhibiting the activation of the complement pathways in subjects with a C5 polymorphism, Coversin also possesses the advantage of being easier to administer than other agents such as antibodies like eculizumab.
The agent is administered in a therapeutically or prophylactically effective amount. The term “therapeutically effective amount” refers to the amount of agent needed to treat the complement-mediated disease or disorder, as defined elsewhere herein. The term “prophylactically effective amount” used herein refers to the amount of agent needed to prevent complement-mediated disease or disorder as defined elsewhere herein. Preferably, the dose of the agent is sufficient to bind as much available C5 as possible in the subject, more preferably, all available C5. The dose of the agent may alternatively be sufficient to bind as much available LTB4 as possible in the subject, more preferably, all available LTB4. In some aspects, the dose of the agent is sufficient to binds as much available C5 and LTB4 as possible, for example all available C5 and LTB4. The dose of the agent supplied may be at least 1× or 1.5 times or twice the molar dose needed to bind all available C5 and/or LTB4 in the subject. The dose of the agent supplied may be e.g. about 1, 1.5, 2, 2.5 times, 3 times or 4 times the molar dose needed to bind all available C5 and/or LTB4 in the subject. Preferably, the dose is from 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, preferably 0.001 mg/kg to 10 mg/kg, preferably 0.01 mg/kg to 2 mg/kg, preferably 0.1 mg/kg to 1 mg/kg; alternatively 0.2 mg/kg to 0.8 mg/kg; alternatively 0.3 mg/kg to 0.7 mg/kg; alternatively 0.4 mg/kg to 0.6 mg/kg; for example 0.14 mg/kg or 0.57 mg/kg. The therapeutically or prophylactically effective amount can additionally be defined in terms of the inhibition of terminal complement, for example, an amount that means that terminal complement activity (TCA) is reduced by at least 10, 20, 30, 40, 50, 60, 70, 80, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100%, compared to terminal complement activity in the absence of treatment. Dose and frequency may be adjusted in order to maintain terminal complement activity at the desired level, which may be, for example 10% or less, for example 9, 8, 7, 6, 5, 4, 3, 2, 1% or less compared to terminal complement activity in the absence of treatment.
Where a dose is given, this relates to a dose of the agent which is a protein or functional equivalent thereof. Appropriate doses for an agent which is a nucleic acid molecule may be used to give rise to these levels.
Terminal complement activity can be measured by standard assays known in the art, e.g. using the Quidel CH50 haemolysis assay and the sheep red blood cell lytic assay.
The frequency with which the dose needs to be administered will depend on the half-life of the agent involved. The Coversin protein or a functional equivalent thereof, may be administered as a continuous infusion, in bolus doses or on a daily basis, twice daily basis, or every two, three, four days, five, six, seven, 10, 15 or 20 days or more. As noted elsewhere, a particular advantage of the Coversin protein and its functional equivalents is the relative ease and rapidity with which it can be administered, and the fact that medical professionals are not required for administration.
Single or multiple doses may be administered. For example at least 2, 3, 4, 5, 6, 7, or 8 doses may be administered. Single doses are one embodiment. The exact dosage and the frequency of doses may also be dependent on the patient's status at the time of administration. Factors that may be taken into consideration when determining dosage include the need for treatment or prophylaxis, the severity of the disease state in the patient, the general health of the patient, the age, weight, gender, diet, time and frequency of administration, drug combinations, reaction sensitivities and the patient's tolerance or response to therapy. The precise amount can be determined by routine experimentation, but may ultimately lie with the judgement of the clinician.
The dosage regimen may also take the form of an initial “loading dose” followed by one or more subsequence “maintenance doses”. In general, the loading dose will be greater than the maintenance dose. The loading dose may be 2, 5, 10 or more times greater than the maintenance dose. The loading dose may be administered as a single dose, or as one or more doses in a particular time frame. Typically, the loading dose will be 1, 2, 3, 4 or 5 doses administered in a single 24 hour period. The maintenance dose may be a lower dose that is repeated at regular intervals. The maintenance dose may be repeated at intervals, such as every 3, 4, 6, 8, 12, 24, or 48 hours. The precise regimen can be determined by routine experimentation, but may ultimately lie with the judgement of the clinician. The maintenance dose may be at least 20, 30, 40, 50, 60, 70, 80, 90 or 100% of the initial loading dose, or up to 20, 30, 40, 50, 60, 70, 80, 90 or 100% of the initial loading dose.
In a further embodiment the same dose is used throughout the course of treatment (e.g. daily).
The loading dose may be 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20 mg/kg; alternatively the loading dose is 0.001 mg/kg to 10 mg/kg and the maintenance dose is 0.001 mg/kg to 10 mg/kg, alternatively the loading dose is 0.01 mg/kg to 2 mg/kg and the maintenance dose is 0.01 mg/kg to 2 mg/kg; alternatively the loading dose is 0.1 mg/kg to 1 mg/kg and the maintenance dose is 0.1 mg/kg to 1 mg/kg; alternatively the loading dose is 0.1 mg/kg to 1 mg/kg and the maintenance dose is 0.05 mg/kg to 0.5 mg/kg; alternatively the loading dose is 0.2 mg/kg to 0.8 mg/kg and the maintenance dose is 0.1 mg/kg to 0.4 mg/kg; alternatively the loading dose is 0.3 mg/kg to 0.7 mg/kg and the maintenance dose is 0.1 mg/kg to 0.3 mg/kg; alternatively the loading dose is 0.4 mg/kg to 0.6 mg/kg and the maintenance dose is 0.1 mg/kg to 0.2 mg/kg for example where the loading dose is 0.57 mg/kg and the maintenance dose is 0.14 mg/kg.
The loading dose may be 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20 mg/kg; alternatively the maintenance dose may be 0.001 mg/kg to 10 mg/kg, alternatively the maintenance dose may be 0.01 mg/kg to 2 mg/kg; alternatively the maintenance dose may be 0.1 mg/kg to 1 mg/kg; alternatively the maintenance dose may be 0.1 mg/kg to 0.8 mg/kg; alternatively the maintenance dose may be 0.1 mg/kg to 0.6 mg/kg; alternatively the maintenance dose may be 0.1 mg/kg to 0.4 mg/kg; alternatively the maintenance dose may be 0.1 mg/kg to 0.2 mg/kg.
The loading dose may be 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, and the maintenance dose may be between 0.0001 mg/kg to 20 mg/kg; alternatively the loading dose may be 0.001 mg/kg to 10 mg/kg, alternatively the loading dose may be 0.01 mg/kg to 2 mg/kg; alternatively the loading dose may be 0.1 mg/kg to 1 mg/kg; alternatively the loading dose may be 0.1 mg/kg to 1 mg/kg; alternatively the loading dose may be 0.2 mg/kg to 0.8 mg/kg; alternatively the loading dose may be 0.3 mg/kg to 0.6 mg/kg; alternatively the loading dose may be 0.4 mg/kg to 0.6 mg/kg. The agent will generally be administered in conjunction with a pharmaceutically acceptable carrier. The term “pharmaceutically acceptable carrier”, as used herein, includes genes, polypeptides, antibodies, liposomes, polysaccharides, polylactic acids, polyglycolic acids and inactive virus particles or indeed any other agent provided that the carrier does not itself induce toxicity effects or cause the production of antibodies that are harmful to the individual receiving the pharmaceutical composition. Pharmaceutically acceptable carriers may additionally contain liquids such as water, saline, glycerol, ethanol or auxiliary substances such as wetting or emulsifying agents, pH buffering substances and the like. The pharmaceutical carrier employed will thus vary depending on the route of administration. Carriers may enable the pharmaceutical compositions to be formulated into tablets, pills, dragees, capsules, liquids, gels, syrups, slurries, suspensions to aid intake by the patient. A thorough discussion of pharmaceutically acceptable carriers is available in [33].
The agent is delivered systemically. The agent may be delivered by a parenteral route (e.g. by injection, either subcutaneously, intraperitoneally, intravenously or intramuscularly or delivered to the interstitial space of a tissue). Other modes of administration include oral and pulmonary administration, suppositories.
Preferably the agent is delivered via subcutaneous injection. In some embodiments this is via once daily subcutaneous injection, for example at an initial loading dose of between 0.0001 mg/kg (mass of drug compared to mass of patient) to 20 mg/kg, followed by once daily maintenance doses of between 0.0001 mg/kg to 20 mg/kg, or other doses disclosed elsewhere herein. Alternatively the agent may be delivered via subcutaneous injection every other day.
In a preferred embodiment the agent is delivered via once daily subcutaneous injection at an initial loading dose of 0.4 mg/kg-0.6 mg/kg (for example 0.57 mg/kg) followed by once daily maintenance doses of 0.1 mg/kg-0.2 mg/kg (for example 0.14 mg/kg).
Preferably the course of treatment is continued for at least 6 weeks, e.g. at least 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 50 weeks or at least 2, 3, 4, 5, 6, 7, 8, 9, 10 months, or at least 1, 2, 3 years. The course of treatment is preferably continued until the subject is no longer considered to be suffering from acute GVHD, or until the subject no longer requires treatment. The course of treatment may thus be administration of the agent (e.g. daily) for at least 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 50 weeks or at least 2, 3, 4, 5, 6, 7, 8, 9, 10 months, or at least 1, 2, 3 years. Daily administration may be continued for at least 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks. The frequency of administration may be modified after at least 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks, e.g. to once every two days.
The maintenance dose (e.g. a single daily maintenance dose) may remain constant throughout the course of treatment) or the maintenance dose (e.g. a daily maintenance dose) may be modified (e.g. increased or decreased) during the course of treatment. The maintenance dose may be modified in order to maintain terminal complement activity at a desired level, e.g. 10% or less compared to serum from said patient in the absence of treatment or compared to normal control serum. The or each maintenance dose may be continued for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks, e.g. daily for at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 weeks. The maintenance dose may be decreased as the subject's symptoms improve. The amount of agent or the frequency with which the agent is administered may be decreased as the subject's symptoms improve.
There may thus be an initial loading dose, followed by an initial maintenance dose (e.g. a daily initial maintenance dose) which may be a maintenance dose as defined above, and one or more further maintenance doses (e.g. a daily further maintenance dose), e.g. at least 2, 3, 4, 5 further maintenance doses.
The invention thus further comprises a method of treating acute GVHD in a subject comprising systemically administering an initial loading dose of the agent as defined above, and then systemically administering maintenance doses (e.g. daily maintenance doses) of the agent as defined above, wherein there is an initial maintenance dose and one or more further maintenance doses.
The invention thus further comprises an agent as defined above for use in a method of treating acute GVHD in a subject, the method comprising systemically administering an initial loading dose of the agent as defined above, and then systemically administering maintenance doses (e.g. daily maintenance doses) of the agent as defined above, wherein there is an initial maintenance dose and one or more further maintenance doses.
The one or more further maintenance doses may be determined by testing the terminal complement activity in the subject (e.g. in a biological sample from the subject), and determining the further maintenance dose for said subject on the basis of the level of terminal complement activity and/or testing the subject's symptoms and determining the further maintenance dose for said subject on the basis of the subject's symptoms. The method may optionally further comprise administering said further maintenance dose. Said further dose may be calculated to be at a level that maintains terminal complement activity at the desired level.
Where a biological sample is taken, this may be blood, e.g. a whole blood or a serum sample. The method optionally further comprises the step of taking the sample, and further optionally comprises the step of determining the TCA of the sample.
The one or more further maintenance doses may be determined by testing the terminal complement activity in the subject (e.g. in a biological sample from the subject), and determining the further maintenance dose for said subject on the basis of the level of terminal complement activity and/or testing the subject's symptoms and determining the further maintenance dose for said subject on the basis of the subject's symptoms. The method may optionally further comprise administering said further maintenance dose. Said further dose may be calculated to be at a level that maintains terminal complement activity at the desired level.
In certain aspects, the desired complement activity level is 10% or less compared to serum from said subject in the absence of treatment or compared to normal control serum.
In certain aspects, if the TCA is higher than the desired level the maintenance dose is increased, and optionally wherein if TCA is less than 5, 4, 3, 2, 1% the dose is maintained or decreased.
In certain aspects, if the symptoms deteriorate the maintenance dose is increased, and optionally wherein if the symptoms improve the dose is maintained or decreased.
In some embodiments the subject is tested within one month of initiating the treatment, within two weeks of initiating the treatment, within a week of initiating the treatment. In other embodiments the subject is tested once a day or at least once a day, once a week, or at least once a week, once every two weeks or at least once every two weeks, once a month or once every two months.
Preferably the loading dose is 0.57 mg/kg or about 0.57 mg/kg protein or functional equivalent and the maintenance dose is at least 0.15 mg/kg (e.g. at least 0.2 mg/kg, 0.15-0.6, 0.2-0.5 or 0.25-0.45 mg/kg) or is up to 0.13 mg/kg (e.g. up to 0.1 mg/kg, 0.05-0.13, 0.075-0.1 mg/kg) and optionally (i) that maintenance dose is continued for at least 2, 3, 4, 5, 6 weeks and/or (ii) treatment is continued for at least 6 weeks and/or (iii) treatment is continued daily for at least 3, 4, 5, 6 weeks.
Preferably the loading dose is 0.4-0.6 mg/kg protein or functional equivalent and the maintenance dose is 0.1-0.2 mg/kg, e.g. about 0.14 mg/kg, and (i) that maintenance dose is continued for at least 2, 3, 4, 5, 6 weeks and/or (ii) treatment is continued for at least 6 weeks and/or (iii) treatment is continued daily for at least 3, 4, 5, 6 weeks.
The present invention relates to the systemic administration of the agents. This means that the agent is present in the circulatory system so that the entire body is affected. This is in contrast with topical administration where the effect is generally local. The systemic presence of the agent can be determined functionally, e.g. on the basis of TCA in the serum of the subject, or directly (e.g. by ELISA or mass spectrometry).
The agent may be administered alone or as part of a treatment regimen also involving the administration of other drugs currently used in the treatment or prophylaxis of patients with acute GVHD, such as Cyclosporine A, Methotrexate, Tacrolimus and glucocorticoids such as prednisone. The agent, alone or with one or more other drug currently used in the treatment or prophylaxis of acute GVHD may be administered at least once before the transplant which gives rise to acute GVHD (e.g. at least one day, or one week or 2 weeks before the transplant), with the transplant or after the transplant (e.g. the initial administration of the agent is up to one day or one week or 2 weeks, or one month or 2 months after the transplant, or up to one day or one week or 2 weeks, or one month or 2 months after diagnosis of acute GVHD)
The agent may be administered simultaneously, sequentially or separately with the other drug(s). For example, the agent may be administered before or after administration of the other drug(s).
Various aspects and embodiments of the present invention will now be described in more detail by way of example. It will be appreciated that modification of detail may be made without departing from the scope of the invention.
The patient was a boy of 4 years old who first presented in 2013 with intermittent abdominal pain and rectal bleeding and was subsequently diagnosed as having chronic granulomatous disease. He underwent haematopoietic stem cell bone marrow replacement from a well-matched donor in October 2013 and received cyclosporine and tacrolimus post operatively. His course was complicated by lower gastrointestinal (GI) bleeding and an initial diagnosis of graft versus host disease (GVHD) was made. Subsequently severe thrombocytopenia developed although ADAMTS13 activity remained normal and antibodies were not detected. Renal function remained normal.
The patient was treated initially with rituximab and infliximab with little improvement. Eculizumab was started in April 2014 but there was no improvement in platelet levels and he continued to need infusion of 2 adult units of fresh platelets daily. Lower GI bleeding continued intermittently and upper GI endoscopy on 14th May revealed ongoing gastritis and duodenitis with thinning and patchy necrosis of the mucosa. Lower GI endoscopy as far as the splenic flexure showed tar-like stools but it was not possible to identify a definite bleeding site. It was considered to be too hazardous to proceed further and the procedure was terminated. Histology of upper GI biopsies confirmed the clinical findings and were considered to be consistent with graft versus host disease.
In late May 2014, after 2 months of weekly infusions of a full adult dose of eculizumab with no clinical improvement, genetic testing revealed that the patient had the sArg885His polymorphism. On 29 May 2014 a serum sample was tested in the Volution complement laboratory at the Haemostasis Research Unit (HRU) at University College, London (UCL) using the Quidel CH50 complement haemolysis assay and spiking with ascending doses of both Coversin and eculizumab. Pooled serum from normal subjects was used as control. The results of this are shown in
This demonstrated that Coversin at c. 15 μg/mL, the known therapeutic concentration, completely ablated terminal complement activity (TCA) in both serum from BJ and in normal control serum. Eculizumab 50 μg/mL ablated TCA in normal control serum but was unable to inhibit TCA beyond c. 80% at any concentration tested. During the eculizumab treatment there was a continuing clinical picture which included thrombocytopaenia, rectal bleeding and serum lactic dehydrogenase (LDH) fluctuating between c.900 and 2,000 IU/L.
A request was made by the responsible physician for Coversin to treat the patient and, following discussions with the patient's parents a limited supply of Coversin was provided for subcutaneous injection and eculizumab was discontinued.
The quantity of clinical grade Coversin available at the time was extremely limited and consisted of the residual from the PIa clinical trial for Coversin. After discussion, despite the limited availability of Coversin, it was decided to commence treatment on empirical grounds with an ablating dose of 0.57 mg/kg and thereafter a daily maintenance dose of 25% of that. Treatment commenced on 1st June 2014 and the initial clinical response appeared to be encouraging with stabilisation of vital signs and reduced rectal bleeding. After 7 daily maintenance doses of Coversin, it was decided to space out the daily maintenance doses to alternate days in order to conserve the supply of Coversin as long as possible, although, at the start of treatment whilst the assay was in development, accurate CH50 assays were not available. By 4th July, 34 days after the start of Coversin treatment it was clear that, although there was clinical improvement compared with the period prior to Coversin, control of TCA was not complete and this was subsequently confirmed by retrospective CH50 assays. A decision was made to increase the dose of Coversin. A further ablating dose of 0.57 mg/kg was given on 5th July and daily maintenance doses of 25% of that were resumed thereafter until Coversin ran out 6 days later.
The LDH results (
The CH50 data indicate that daily dosing with Coversin at 25% of the ablating dose controlled TCA.
During the 58 days that the patient received Coversin there were no reported adverse events attributable to the drug and no injection site reactions. Low titre anti-Coversin antibodies (IgG) were detected after 14 days treatment but were non-neutralising.
This case shows that people of non-Japanese/Chinese origin may also be affected by the polymorphisms previously described only in these ethnic groups by Nishimura, J et al., New Engl J. Med., 30; 7: 632-639 (2014).
Elevated serum LDH is an early indicator of TMAs and occult malignancy and is a good indicator of response to therapy [34]. In this case, although it never fully returned to normal levels, there was a clear indication that during the two periods when an adequate dose of Coversin was being given it fell suggesting that red cell and platelet destruction and mucosal damage were diminishing. By contrast, during the two months of eculizumab treatment, the LDH remained generally above 1,000 IH/L and extremely variable.
Coversin completed a single dose Phase I clinical trial in 2014 in normal volunteers by subcutaneous injection. At a dose of 0.57 mg/kg the drug caused total blockade of complement haemolytic activity in all subjects at 12 hours post-dose returning to ˜50% activity by 48 hours.
The Quidel Microvue CH50 Eq enzyme immunoassay (cat #A018) is for in vitro measurement of total classical pathway activity in human serum. http://www.quidel.com/sites/quidel.com/files/productldocuments/a018_microvue_ch50_eq_english_1.pdf
The kit provides a direct measure of the terminal complement complex (TCC) formation under standard conditions. Measurement of CH50 with the kit has 3 steps:
1. Activation of the classical complement pathway in undiluted serum resulting in formation of TCC.
2. Dilution of serum and addition to microassay wells coated with an antibody that captures TCC.
3. Quantification of captured TCC with and anti-TCC horse radish peroxidase (HRP) conjugated antibody.
Colour intensity on addition of substrate is proportional to the concentration of TCC present in each reaction. Using the kit standard curve (determined during each assay) assay results are expressed in CH50 unit equivalents per millilitre (CH50 U Eq/ml).
The linear range for the kit is 30-310 CH50 U Eq/ml.
According to the manufacturers the cut off for normality determined from 234 individual human samples is 70 CH50 U Eq/ml.
Sample Preparation: Serum was prepared by collecting blood into plain glass or SST Vacutainer tubes (or equivalent) and allowing it to clot for 1 hour, before centrifugation at 1500 g for 10 minutes. The serum was separated immediately (avoiding contamination with any blood cells) and stored in screw cap cryotubes (approximately 0.5 ml aliquots) at −70° C.
Coversin: Frozen 10.9 mg/ml solution at −70° C. Dilute 10 uL in 90 uL normal control or patient serum to give a final concentration of 1.09 mg/ml. Dilute 10 uL in 90 uL autologous serum to give a final concentration of 109 ug/ml. Double dilute in autologous serum to achieve a final concentration range of: 0.4-54.5 ug/ml.
Eculizumab: Frozen solution of 10 mg/ml. Dilute 10 uL in 90 uL normal control or patient serum to give a final concentration of 1 mg/ml. Dilute 10 uL in 90 uL autologous serum to give a final concentration of 100 ug/ml. Double dilute in autologous serum to achieve a final concentration range of: 0.4-50 ug/ml.
Buffer: Phosphate Buffered Saline (0.01M phosphate buffer, 0.0027M potassium chloride, 0.137M sodium chloride, pH7.4).
Coversin, Eculizumab, or buffer (control), are spiked into serum according to the procedure above to achieve a range of final concentrations. These are then assayed for CH50 Equivalent activity using the Quidel CH50 kit, using duplicate wells.
CH50 values are calculated from the calibration curve provided with the kit and plotted as raw CH50 values against C5 inhibitor concentration. The CH50 results are calculated at each C5 inhibitor concentration as a percentage of the CH50 concentration of the relevant buffer control and plotted against inhibitor concentration.
Repeat the experiment on separate days to obtain 3 measurements in each patient and in a single normal control. This provides an estimate of between experiment variability.
Repeat the experiment on separate days in single experiments on 6 different normal controls. This provides an estimate of between subject responsiveness (and avoids the risk of using a single subject who may have an unknown C5 mutation or polymorphism).
The highest dose of each drug to whole serum was added and then two-fold serial dilutions were made in whole serum. One replicate was used for each drug dose.
After serial dilution the serum was activated and assayed in accordance with the instructions for the Quidel CH50 kit.
CH50 U Eq/ml were calculated in comparison with the kit standards and plotted against drug concentration for each of the three serum samples and two drug treatments. They were also plotted as a percentage of the CH50 value of the relevant buffer only control.
| Number | Date | Country | Kind |
|---|---|---|---|
| PCT/EP2015/062742 | Jun 2015 | EP | regional |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/EP2015/079172 | 12/9/2015 | WO | 00 |
