Claims
- 1. A process for the fermentation of an oxygen-dependent microorganism, which comprises:
- a) inoculating a fermentation broth with said oxygen-dependent microorganism;
- b) initially supplying a carbon source to said inoculated fermentation broth at a rate proportional to the oxygen uptake rate of said oxygen-dependent microorganism until a threshold value is reached, wherein said threshold value is that value at which no carbon oversupply takes place; and
- c) after the threshold value is reached, providing said carbon source to said inoculated fermentation broth at a constant supply until fermentation is finished.
- 2. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein said oxygen-dependent microorganism is modified by genetic engineering to express a recombinant protein.
- 3. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein said oxygen-dependent microorganism belongs to the species Escherichia coli.
- 4. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 3, wherein said oxygen-dependent microorganism is of the strain W 3110 M or DH 1.
- 5. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein glutaryl acylase is produced.
- 6. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein the inoculation of the microorganism in step (a) takes place by means of a preculture.
- 7. The process of the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein the inoculation of the microorganism in step (a) takes place by a direct inoculation.
- 8. The process for the fermentation of an oxygen dependent microorganism as claimed in claim 1, wherein the supply of the carbon source takes place in a computer-controlled manner as a function of the oxygen uptake rate.
- 9. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 1, wherein the carbon source supplied to the microorganism is glycerol.
- 10. The process for the fermentation of an oxygen-dependent microorganism as claimed in claim 9, wherein said threshold value is that value at which no formation of acetate takes place.
- 11. The process as claimed in claim 1 wherein the oxygen uptake rate of step (b) and the carbon source supply of step (c) are determined empirically.
Priority Claims (1)
Number |
Date |
Country |
Kind |
43 37 787.4 |
Nov 1993 |
DEX |
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Parent Case Info
This application is a continuation of application Ser. No. 08/335,380, filed Nov. 3, 1994, now abandoned.
US Referenced Citations (6)
Foreign Referenced Citations (9)
Number |
Date |
Country |
A-1278619 |
Jun 1972 |
EPX |
A-144474 |
Jun 1985 |
EPX |
A-196061 |
Oct 1986 |
EPX |
A-504798 |
Sep 1992 |
EPX |
A-1953430 |
Apr 1970 |
DEX |
A-159011 |
Feb 1983 |
DEX |
0290212 |
May 1991 |
DEX |
0595441 |
Feb 1978 |
SEX |
A-297444 |
Jan 1992 |
GBX |
Non-Patent Literature Citations (2)
Entry |
Derwent Abstract of DD-A-159011, see abstract 1981. |
R. Gherna et al., Catalogue of Bacteria and Phages,American Type Culture Collection, 18th Ed., pp. 135, 137 (1992). |
Continuations (1)
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Number |
Date |
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Parent |
335380 |
Nov 1994 |
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