Claims
- 1. A murein binding polypeptide in vitro diagnostic reagent for detecting a eubacteria or a fungus in a biological sample, wherein
- said murein binding polypeptide in vitro diagnostic reagent comprises a murein binding polypeptide chemically conjugated to a signal generating compound;
- said murein binding polypeptide comprises a catalytically inactive enzyme polypeptide having a binding site capable of binding to either a eubacterial murein compound or a fungal murein like compound with a binding affinity of about 5.times.10.sup.-7 L/mol to about 5.times.10.sup.-9 L/mol;
- and wherein said conjugate comprising said murein binding polypeptide and signal generating compound is effective when bound to said eubacteria or said fungus to produce a detectable signal in a diagnostic assay format.
- 2. The murein binding polypeptide in vitro diagnostic reagent of claim 1, wherein said signal generating compound is selected from the group consisting of an enzyme, a fluorophore, a phycobilin, a biotin, an avidin, a streptavidin, a bioluminescent compound, a chemiluminescent compound, a histochemical dye, and a radiolabeled compound.
- 3. The murein binding polypeptide in vitro diagnostic reagent of claim 1, wherein said catalytically inactive enzyme polypeptide comprises a derivative of a wild-type enzyme selected from the group consisting of a mutant enzyme, a recombinant enzyme, and a chemically inactivated enzyme.
- 4. The murein binding polypeptide in vitro diagnostic reagent of claim 3, wherein said wild-type enzyme polypeptide is selected from the group consisting of an acetyl-muramoyl-D,L-Alanyl amidase, a bacterial cell wall penicillin binding protein, an alanyl D,D- or D,L-endopeptidase, a D,D- or D,L-carboxypeptidase, a transglycosyl transferase, a peptidyl transferase, a muramoyl isomerase, a muramoyl transglycosylase, a murein autolysin, a murein hydrolase, and a lysozyme.
- 5. The murein binding polypeptide in vitro diagnostic reagent of claim 1, further comprising an additive selected from the group consisting of a stabilizer, a buffer, an emulsifier and an agent for promoting a binding interaction between said murein binding polypeptide and said murein or said murein like compound.
- 6. The murein binding polypeptide in vitro diagnostic reagent of claim 1, wherein said catalytically inactive enzyme polypeptide comprises a portion of a wild-type enzyme having said binding site.
- 7. A murein binding polypeptide in vitro diagnostic reagent for detecting a eubacteria or a fungus in a biological sample, wherein
- said murein binding polypeptide in vitro diagnostic reagent comprises a multimeric murein binding polypeptide chemically conjugated to a signal generating compound;
- said multimeric murein binding polypeptide comprises two or more murein binding polypeptides each independently comprising a catalytically inactive enzyme polypeptide having a binding site capable of binding to either a eubacterial murein compound or a fungal murein like compound with a binding affinity of about 5.times.10.sup.-7 L/mol to about 5.times.10.sup.-9 L/mol;
- wherein said conjugate comprising said murein binding polypeptide and signal generating compound is effective when bound to said eubacteria or said fungus to produce a detectable signal in a diagnostic assay format.
- 8. The murein binding polypeptide in vitro diagnostic reagent of claim 7, wherein said two or more catalytically inactive enzyme polypeptides each comprise a derivative of a wild-type enzyme selected from the group consisting of a mutant enzyme, a recombinant enzyme, and a chemically inactivated enzyme.
- 9. The murein binding polypeptide in vitro diagnostic reagent of claim 8, wherein said two or more murein binding polypeptides comprise derivatives of different wild-type enzymes.
- 10. The murein binding polypeptide in vitro diagnostic reagent of claim 9, wherein said two or more different catalytically inactive enzymes comprises a chimeric polypeptide.
Parent Case Info
This application is a division of U.S. patent application Ser. No. 08/823,293 filed Mar. 21, 1997, now U.S. Pat. No. 5,935,804.
US Referenced Citations (5)
Foreign Referenced Citations (1)
Number |
Date |
Country |
9217786 |
Oct 1992 |
WOX |
Non-Patent Literature Citations (1)
Entry |
Hough et al., "Ubiquitin-Lysozyme Conjugates, Purification and Susceptibility to Proteolysis", J. Biol. Chem., 261, pp. 2391-2399, Feb. 1987. |
Divisions (1)
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Number |
Date |
Country |
Parent |
823293 |
Mar 1997 |
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