Patent Application
20180236107
The invention relates to a composition for use in the field of medical imaging, and particularly, but not exclusively, to a particle composition for use in detecting or diagnosing a protein-related, neurodegenerative disease in a subject under Magnetic Resonance Imaging (MRI), such as, but not limited to, Alzheimer's disease (AD).
Alzheimer's disease (AD) is a progressive neurodegenerative disorder which presents the highest prevalence rate among a number of dementing diseases. The disease may initially affect cognitive abilities such as memory loss and other intellectual abilities, and in the advanced stage, patients may suffer from the loss of motor functions and may require assistance from others in performing basic, daily life activities. Recent studies have revealed that, among the 40 million people worldwide who suffer from dementia, around 60% to 80% of these cases have been classified as Alzheimer's disease. Although the risk factor of Alzheimer's disease is known to increase with age, about 5% of the total AD patients are diagnosed with early onset, i.e. with symptoms first appearing before 65 years of age. As life expectancy continuously rises, it is predicted that the prevalence of AD will drastically increase in the near future.
Despite advancements in medical technology, Alzheimer's disease (AD) is still considered incurable, with the drugs currently available only providing partial relief of the symptoms, but which do not slow down or reverse the degeneration of brain functions caused by the disease. Although the causes of AD have not been clearly identified, one commonly known hypothesis is that the disease may be related to or caused by the abnormal aggregation of beta amyloid (Aβ) proteins in brain tissues of the patient. The ability to identify or detect the presence of these beta amyloid (Aβ) aggregates at an early stage is therefore of significant importance to the diagnosis and treatment of the disease.
Nevertheless, available methods for diagnosing Alzheimer's disease are found to be inadequate and inefficient. For example, a radioactive positron emission tomography (PET) imaging reagent that binds to amyloid plaques was recently approved for clinical use. However, it is known to suffer from the short-comings of high cost, limited availability, toxic radioactivity and low spatial resolution such that individual plaques are difficult if not impossible to be clearly visualized. Another approach which uses Magnetic Resonance Imaging (MRI) to diagnose Alzheimer's disease involves the use of potentially toxic reagents which limit the maximum safe dosage administrable to a patient and accordingly, limits the sensitivity and accuracy of the method.
It is further reported by K K Cheng et al. in Biomaterials 44 (2015) 155-172 published on 12 Jan. 2015 and U.S. provisional application 62/496,757 that iron oxide particles with surfaces coated with curcumin, a naturally occurring compound extracted from turmeric, the root of the Curcuma longa plant, demonstrated ability to bind to beta amyloid (Aβ) plaques in brain tissues of mice and which are detectable under MRI. However, the technique still shows insufficiencies in terms of signal sensitivity and spatial resolution in practice for Alzheimer's disease detection, as well as the process in preparing the particles.
An object of the present invention is to provide an agent for use in medical imaging such as, but not limited to, MRI for identifying one or more neurodegenerative diseases or conditions related protein molecules.
Another object of the present invention is to provide an agent for use in MRI for diagnosing one or more neurodegenerative diseases in a subject such as, but not limited to, Alzheimer's disease.
A further object of the present invention is to mitigate or obviate to some degree one or more problems associated with known imaging techniques for brain or brain tissues, or at least to provide a useful alternative.
The above objects are met by the combination of features of the main claims; the sub-claims disclose further advantageous embodiments of the invention.
One skilled in the art will derive from the following description other objects of the invention. Therefore, the foregoing statements of object are not exhaustive and serve merely to illustrate some of the many objects of the present invention.
In general, the invention provides an agent for use in medical imaging such as, but not limited to, Magnetic Resonance Imaging (MRI) for identifying, showing, determining, or more specifically, allowing or enhancing visibility under the imaging technique of one or more proteins or protein molecules relating to one or more neurodegenerative diseases or conditions such as, but not limited to, Alzheimer's Disease (AD). Particularly, the present invention provides a composition comprising metal ion-metal ion chelating agent complex nanoparticles identifiable under the MRI. In one preferred embodiment, the composition comprises curcumin-iron metal ion complex nanoparticles capable of binding with one or more of an aggregate, plaque, tissue, deposit, fibril, oligomer and/or molecule of any forms of beta amyloid (Aβ) proteins. In the context of the present description, the expression “metal ion-metal ion chelating agent complex” relates to a complex formed by complexation between one or more metals or metal atoms with one or more metal ion chelating agents; and the expression “curcumin-iron metal ion complex” relates to a complex formed by complexation between one or more curcumins, compounds of curcumin, curcumin derivatives and/or a mixture thereof with one or more iron metal ions.
Curcumin is a naturally occurring botanical extract and has been studied for its potential applications in the treatment of a number of diseases and conditions in human and animal subjects due to their anti-inflammatory, antioxidizing, anti-cancer and metal ion-chelating properties. Particularly, curcumin has been shown to be capable of binding with beta amyloid (Aβ) aggregates and thus, has demonstrated potential to be used as indicators for Alzheimer's disease based on a well-accepted hypothesis that the disease is likely to be related to or caused by the abnormal aggregations of beta amyloid (Aβ) proteins in brain tissues.
In contrast to the prior technologies which allow tagging or labelling of beta amyloid (Aβ) plaques in brain tissues and thus are capable of revealing the affected regions at low sensitivity and spatial resolution under MRI, the present invention is advantageous in providing metal ion complex nanoparticles of sufficiently small size which enable binding of the metal ion comprising nanoparticles not only to the relatively large targets of beta amyloid (Aβ) plaques, but also beta amyloid (Aβ) fibrils or even oligomers which are typically too small to be effectively traceable or taggable in practice. The present invention therefore demonstrates a significant improvement to the current technology which allows the screening and/or diagnostic of AD at a much earlier stage. The present invention also provides a relatively simple and inexpensive method in preparing the metal ion complex nanoparticles, with high reproducibility and stability achievable.
In a first main aspect, the invention provides a particle composition for use as a contrasting agent in medical imaging. The particle composition comprises a metal ion containing compound comprises at least one metal ion complexed with at least one metal ion chelating agent; wherein the metal ion chelating agent is adapted to bind to one or more protein molecules to thereby allow or enhance visibility of the protein molecules under the medical imaging.
In a second main aspect, the invention provides a diagnostic agent for use in magnetic resonance imaging (MRI), comprising the particle composition according to the first main aspect.
In a third main aspect, the invention provides a method of preparing a particle composition. The method comprises the steps of: providing a metal ion containing compound comprises at least one metal ion complexed with at least one metal ion chelating agent; dissolving the metal ion containing compound in an organic solvent to form an organic phase; introducing an amphiphilic copolymer to the organic phase to form a mixture; mixing an aqueous phase with the mixture thereby forming core-shell particles each having a metal ion-metal ion chelating agent complex core and an amphiphilic copolymer comprising shell.
The summary of the invention does not necessarily disclose all the features essential for defining the invention; the invention may reside in a sub-combination of the disclosed features.
The foregoing and further features of the present invention will be apparent from the following description of preferred embodiments which are provided by way of example only in connection with the accompanying figure, of which:
The following description is of preferred embodiments by way of example only and without limitation to the combination of features necessary for carrying the invention into effect.
Reference in this specification to “one embodiment” or “an embodiment” means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment of the invention. The appearances of the phrase “in one embodiment” in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. Moreover, various features are described which may be exhibited by some embodiments and not by others. Similarly, various requirements are described which may be requirements for some embodiments but not other embodiments.
The present invention relates to a particle composition for use in medical imaging such as, but not limited to, Magnetic Resonance Imaging (MRI) for identifying, showing, determining, or more specifically, allowing or enhancing visibility of one or more proteins or protein molecules relating to one or more neurodegenerative diseases or conditions such as, but not limited to, Alzheimer's Disease (AD). The particle composition can be potentially applied as, for example, a contrasting agent, a diagnostic agent, an indicator and/or a probe during the medical imaging process of a subject. Particularly, the particle composition can be used to allow, enhance and/or improve visibility and/or contrast of structures and/or regions of interests as shown by the medical images generated, and/or to detect, determine and/or reveal certain conditions and/or functions of the structures and/or regions being examined under the medical imaging process.
Specifically, the particle composition can be used with, for example, MRI, for visualizing, enhancing the received signal of, and/or determining or identifying the presence of one or more proteins or aggregates of the proteins, and preferably, one or more proteins or aggregates of the proteins relating to one or more neurodegenerative diseases or conditions. In one embodiment, the proteins may comprise, but are not limited to beta amyloid (Aβ) proteins. The particle composition may further be applied for diagnosing one or more protein-related neurodegenerative diseases or conditions such as, but not limited to, Alzheimer's disease, Parkinson's disease, Lewy body disease, and/or other dementing conditions, etc.
Although the technique of medical imaging described and exemplified herein relates mainly to Magnetic Resonance Imaging (MRI), it will be appreciated that the present invention should not be restricted to the application of MRI, but any other means of medical imaging, techniques, instruments and tomographic scanners or the like, whether or not they are primarily designed to generate an image as such, shall also be encompassed, as long as they are considered suitable and applicable for the present invention without departing from the inventive concept.
Referring to
In the context of the present description, the terms “complex”, “complexing” and/or “complexation” generally relate to a physical attraction or chemical reaction such as, but not limited to, ionic binding between one or more ligands with one or more metal ions to thereby combine the one or more individual ions, atoms and/or molecules to become a larger group or aggregate of ions, atoms and/or molecules. For example, in one specific embodiment as described herein, the iron ions or iron atoms are allowed to bind with the curcumin ligands to thereby enable the iron-curcumin complex to grow in size and thus forming the iron-curcumin complex particle. Throughout the iron-curcumin complex particle, the curcumin and the iron metal ion are preferably randomly located as a cluster due to the ionic attractions therebetween, for example, as shown in
Curcumin is also known to be able to bind to various amyloid proteins including beta amyloid, although the mechanism causing the binding is not yet clearly understood. In the present invention, the ability for curcumin to bind with both the iron ions and also, beta amyloid at different regions of the curcumin ligand molecule allows the beta amyloid to become visible or visible with enhanced contrast or resolution under the MRI due to the characteristic of the curcumin-iron complexes as being magnetisable under the magnetic field of the MRI. In one embodiment, the curcumin-iron complexes demonstrate single-domain magnetism, which is known to provide an enhanced magnetic signal when compared with other forms of magnetization such as super paramagnetism.
In particular, the very small size of the resulting nanoparticles as achieved by the present invention, for example, with an average diameter equal to or smaller than about 100 nm, and preferably, equal to or smaller than about 50 nm, enables binding of the particles to fibrils or oligomers of the beta amyloid protein. This facilities diagnosis of the relevant beta amyloid related diseases or conditions, such as Alzheimer's disease, at their early stage.
In one embodiment, the metal ion containing compound of the particle composition can be magnetisable under at least one of an electromagnetic wave, an electric field and a magnetic field; preferably, under a magnetic field with a strength ranged from about 1 Tesla (T) to about 9 T; and more preferably, about 3 T for an MRI operation on a human subject. The metal ion may comprise one or more of a ferromagnetic metal ion or metal ion alloy, such as but not limited to, iron ion. Alternatively, the metal ion may also comprise one or more transition metals or metal ions, such as but not limited to, iron, zinc and/or copper etc. in their metallic or ionic forms, as long as they are capable of complexing with the metal ion-chelating agent such as curcumin. The metal ion chelating agent may comprise one or more synthetic, naturally occurring, and/or naturally derived compounds, such as but not limited to, curcuminoids, flavonoids, resveratrols, compounds or derivatives thereof and/or mixture thereof. In one embodiment, the metal ion chelating agent may comprise synthetic molecules or compounds which are designed to mimic one or more of a naturally occurring and a naturally derived compounds. The metal ion chelating agent are capable of binding with one or more metal atoms and/or ions such as, but not limited to, one or more of iron, copper, and/or zinc in their metallic or ionic forms. Alternatively, the metal ion chelating agent can be a natural or synthetic compound comprising one or more metal binding moieties or functional groups such as, but not limited to, phenol, keto and/or hydroxyl groups, and preferably, be a polyphenol bearing compound, with the binding moieties or functional groups being capable of binding or interacting with beta amyloid (Aβ) proteins.
Preferably, the metal ion containing compound comprises an iron metal ion-curcumin complex, i.e. as a result of an ionic complexation process between iron atoms and/or ions with curcumin, to form one or more particles or particulates; and more preferably, each with an average size of equal to or smaller than about 100 nm in diameter, and more preferably, equal to or smaller than about 50 nm.
In one embodiment, the iron-curcumin complex particles are preferably surrounded by amphiphilic copolymers thereby forming particles with core-shell structures, i.e. each comprising an iron-curcumin complex core surrounded by an amphiphilic copolymers comprising shell, as shown in
In one further embodiment, the core-shell nanoparticles may further be stabilized by using a stabilizing agent such as a surfactant. The surfactant may be selected from a group consisting of polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), polyethylene glycols (PEG), hydroxypropyl methylcellulose (HPMC), poloxamers, polylactic-co-glycolic acid (PLGA), poly caprolactone (PCL), polylactic acid (PLA), poly(butyl)cyanoacrylate (PBCA), chitosan and a mixture thereof. The stabilization of the core-shell structure can be achieved by using PVP as illustrated in
Despite the above described examples of amphiphilic copolymers and surfactants, one skilled in the art will appreciate that the present invention should not be restricted to the specific examples provided, but any other compounds, reagents or the like shall also be encompassed as long as they are considered suitable and applicable for the present invention without departing from the inventive concept.
Preferably, the stabilized core-shell particles are of an average size equal to or smaller than about 50 nm in diameter. For example, the TEM of
In another aspect, the present invention relates to a diagnostic agent for use in medical imaging such as, but is not limited to, MRI and that the diagnostic agent comprises the particle composition of any one of the embodiments as herein described.
In one further aspect, the present invention also relates to a method of preparing one or more of the above described embodiments of the particle composition. The method may comprise the steps of, for example, providing a metal ion containing compound comprises at least one metal ion such as, but not limited to, iron metal ion complexed with at least one metal ion chelating agent such as, but not limited to, curcumin thereby forming an iron-curcumin complex. Preferably, the complex is prepared by having iron and curcumin in a ratio ranging from about 1:2 to about 1:5, and more preferably, iron and curcumin in about 1:3 mole ratio, for example.
The iron-curcumin complex can be formed by various complexing mechanisms including, for example, complexation due to ionic attraction between the metal ion chelating agent and one or more metal ions. Preferably, the iron-curcumin complex is prepared in the form of particles with an average size equal to or smaller than about 100 nm in diameter, or more preferably, equal to or smaller than about 50 nm in diameter.
The method may further comprise dissolving the iron-curcumin complex in an organic solvent to form an organic phase. The organic solvent can be any solvents capable of substantially or fully dissolving the iron-curcumin complex, which can be, for example, dimethyl formamide (DMF), dimethyl sulfoxide (DMSO), acetone, ethanol or a mixture thereof. Following the preparation of the iron-curcumin complex containing organic phase, the next step is to introduce one or more amphiphilic copolymers, as described earlier, to the iron-curcumin complex containing organic phase. In one embodiment, this can be done by introducing or dissolving the copolymers directly to the organic phase to form an organic mixture. Alternatively, the copolymers can be priorly dissolved in another solvent and subsequently, added the copolymer solution into the iron-curcumin complex containing organic phase.
The core-shell structure of the nanoparticles can then be achieved by mixing in an aqueous phase, which may comprise phosphate buffered saline (PBS) or an isotonic solution comprising, for example, 0.9% sodium chloride solution, 0.45% sodium chloride solution with 2.5% dextrose, 5% dextrose solution, or the like, with the iron-curcumin complex/copolymers containing organic mixture. Based on the solubility difference between the two phases, a self-assembly of the amphiphilic copolymers will be induced thereby forming the core-shell structures, i.e. each comprising an iron-curcumin complex core and an amphiphilic copolymer comprising shell. In one embodiment, the aqueous phase may comprise, for example, only water or deionized water.
Preferably, the method may further comprise a step of stabilizing the formed core-shell particles by at least one stabilizing agent such as a surfactant, as described above. More preferably, the surfactant is dissolved in an aqueous medium such as water, deionized water, isotonic solution or PBS prior to reacting or mixing with the formed core-shell particles to thereby stabilize the formed core-shell particles. In one embodiment, the surfactant can be priorly dissolved in the aqueous phase of the previously step, i.e. the core-shell structure forming step.
In one further embodiment, the mixing between the aqueous phase with the iron-curcumin complex containing organic phase may be conducted via a mixing device 10 having at least two inlets 20A, 20B and at least one outlet 30, as shown in
For example, in one specific embodiment, the iron-curcumin complex containing organic mixture and the aqueous phase can be separately introduced via the two respective inlets 20A, 20B into the mixing device 10 to thereby form a solution mixture. Due to the solubility difference, the amphiphilic copolymers will self-assemble with the iron-curcumin complex to form nanoparticles with the core-shell structures, i.e. each having an iron-curcumin complex core and an amphiphilic copolymer comprising shell. The solution mixture will then be discharged via the outlet 30, with the solution mixture optionally collected in an aqueous medium comprising, for example, PVP dissolved in deionized water. The PVP aqueous solution is adapted to stabilize the formed core-shell structure, as well as to reduce a size of the formed core-shell particles.
The following examples illustrate exemplified processes with steps for preparing one or more of the embodied particle composition as described above.
180 mg of iron nitrate (Fe(NO3)3.9H2O; 0.45 mmol) was weighed and dissolved in 5 mL of dimethylformamide (DMF). Separately, 500 mg of curcumin (1.36 mmol) was weighed and dissolved in 25 mL of DMF in a 200 mL round-bottom flask. Both of the solutions were sonicated until the iron nitrate and the curcumin were completed dissolved. The content of iron nitrate and curcumin are prepared in a 1:3 mole ratio.
The iron nitrate solution was then added dropwise to the curcumin solution, with color of the mixture slowly turning from deep orange to brown showing the formation of the complex. The round bottom flask was then wrapped with aluminum foil to ensure the reaction carried out under darkness, i.e. in the absence of light. The reaction was carried out at room temperature overnight.
After the overnight reaction, the reaction mixture was lyophilized and a viscous gel was obtained. 100 mL of deionized water was added to the flask and it was sonicated for 10 minutes for washing. Deep brown precipitate was observed during the sonication. The mixture was then transferred to 50 mL centrifuge tube and was centrifuged at 7800 rpm at 4° C. for 10 minutes. The supernatant was discarded and slurry precipitate was obtained. This washing process with centrifugation was repeated twice with deionized water to remove the nitrate salts.
About 10 mL of dichloromethane (DCM) was added to the slurry precipitate and the mixture was sonicated for 1-2 minutes. The mixture was then centrifuged and the supernatant was discarded. This washing process was repeated once to remove any unbound iron and/or curcumin. After the washing process, the powder was lyophilized by using a freeze dryer overnight. Deep brown powder of the iron-curcumin complex was obtained.
Preparation of the Core-Shell Nanoparticles with Iron-Curcumin Complex Cores and Amphiphilic Diblock Copolymer Shells Stabilized by PVP 40 mg of the prepared iron-curcumin complex powder was weighed and dissolved in 8 mL of DMF to obtain a solution at a concentration of 5 mg/mL. Subsequently, 80 mg of PEG(2k)-b-PLA(10k) diblock copolymer was weighed and transferred to the iron-curcumin complex/DMF solution. The content of the iron-curcumin complex powder and the PEG(2k)-b-PLA(10k) diblock copolymer were prepared in 1:2 mass ratio. The mixture was sonicated for 10 minutes to ensure the diblock copolymer was completely dissolved. The solution was then filtered by a 0.45 μm PTFE filter.
0.3% of polyvinylpyrrolidone-K30 (PVP-K30) solution was separately prepared by dissolving 0.3 g of PVP-K30 into 100 mL deionized water to form a PVP aqueous solution.
7 mL of the iron-curcumin complex/diblock copolymer/DMF solution and 7 mL of deionized water were separately prepared in two 10 mL syringes, respectively. The syringes were then connected to the T-joint mixing device 10 (as shown in
Core-shell nanoparticles having iron-curcumin complex cores and diblock copolymer shells were prepared through T-joint mixing device 10 under a pump flow rate of 35 mL/min. The initial 5 mL of the liquid mixture, i.e. 2.5 mL from each of the syringes, was discarded. Then the subsequent 5 mL of the liquid mixture comprising the core-shell nanoparticles was collected with a container with 45 mL of 0.3% PVP-K30 solution. The resulting solution was stirred at 400 rpm to form the PVP stabilized core-shell nanoparticles. Clear orange solution was obtained and the formed nanoparticles were characterized by a particle size analyzer.
The mixture having the formed PVP stabilized core-shell nanoparticles was purified by dialysis (Spectra/Por 1 Dialysis Tubing, 6-8 kD MWCO) against deionized water, stirred at 300 rpm at 4° C., to thereby remove the residual DMF in the solution. The deionized water was changed every hour for the first 3 hours and then the dialysis was continued overnight. The purified core-shell nanoparticles were then collected and stored at 4° C.
The synthesized core-shell nanoparticles particles were characterized by nanoparticle size and zeta potential analyzer (Malvern Zetasizer Nano-ZS90), with the measured particle sizes and polydispersity indexes (PDI) for three separate batches of nanoparticles synthesized under the same formulation and condition being shown in Tables 1 and 2 below. The characterization revealed a mean particle size of 55.34 nm, with a narrow mean PDI of 0.178 for the three batches of the core-shell nanoparticles. The relatively low standard deviations (SD) and % relative standard deviations (%RSD) also support the high reproducibility of the nanoparticles achievable by the preparation method of the present invention.
Magnetic Resonance Imaging (MRI) of Amyloid Proteins Targeting by the Synthesized Core-Shell Nanoparticles with Iron-Curcumin Complex Cores and Amphiphilic Diblock Copolymer Shells Stabilized by PVP
The synthesized, PVP stabilized core-shell nanoparticles having iron-curcumin complex cores and amphiphilic diblock copolymer shells were tested in AD diseased mice for their potential targeting of beta amyloid (Aβ) protein aggregates in brain tissues under the MRI. Specifically, core-shell nanoparticles (400 μl of nanoparticle suspension which contains 3.75 mg Fe/ml) prepared in accordance with the above described methods, were administered via intravenous (IV) injection into an AD diseased mouse. The brain of the mouse was scanned in vivo under a 2-D FLASH MRI 4 hours after the injection. A healthy, non-AD diseased mouse was also injected with the core-shell nanoparticles prepared under the same condition, and was scanned under the same MRI setting as a control experiment. Both the AD diseased mouse and the healthy, control mouse are of about 7 months old. Both mice were anesthetized (5 ml/kg body weight) with a mixture of 100 mg/ml ketamine and 10 mg/ml xylazine in phosphate-buffered saline (PBS). For each of the mice, skin of the left leg was cut open to expose the femoral vein. Injection of the nanoparticle suspension to the mice was done using the smallest gauge needle (G30), and the injection area was magnified using a magnifying glass. After the injection, the cut in the left leg of each of the mice was sewn up, and the mice were kept for 4 hours prior to the MRI scan. During the MRI scan, the mice were anesthetized by inhalation of isoflurane mixed with air, and their body temperatures were kept at 37° C. by temperature-controlled water blanket. Both mice were scanned by 7-tesla MRI, with the parameter settings as follows:
Serial MRI images produced from whole brain scans on the AD diseased mouse and the control mouse are shown in
The full brain scan of the AD diseased mouse revealed a number of dark spots in various sizes, shapes and darkness and are consistently visible in every one of the MRI images from positions #1 to #15. No dark spots are seemed to be present in the MRI images from the corresponding scans of the healthy, non-AD diseased mouse. The dark spots are believed to be caused by the accumulation or localization of the core-shell iron-curcumin complex nanoparticles of the present invention, which indicate the presence of beta amyloid (Aβ) proteins or aggregates of such proteins under the MRI due to the strong affinity of the nanoparticles with beta amyloid (Aβ) proteins. In other words, the beta amyloid (Aβ) protein aggregates in the brain tissues of the AD diseased mouse are found to be successfully taggable by the core-shell iron-curcumin complex nanoparticles, with the iron-curcumin complex core of the nanoparticles being visualisable or identifiable under the MRI due to the magnetizability of the iron-curcumin complex.
The nanoparticles of the present invention thus serve as a useful means to indicate the presence of and/or to provide location information of amyloid (Aβ) protein aggregates under the MRI, with potential applications in the detecting or diagnosing of Alzheimer's disease in animal and human subjects, as well as to allow, improve or enhance visibility and/or contrast of the detected amyloid (Aβ) aggregates under the MRI. The sufficiently small size of the core-shell nanoparticles, with the core having a diameter of less than 10 nm in general, further enable their binding not only to the relatively large targets of the beta amyloid (Aβ) plaques in the brain tissues, but also beta amyloid (Aβ) fibrils and/or oligomers. It is known that beta amyloid (Aβ) fibrils and/or oligomers are typically too small to be effectively traceable and/or taggable by any traditional techniques, and therefore the ability for the present invention to identify or detect the presence of these beta amyloid (Aβ) fibrils and oligomers represent a significant improvement to the diagnosis and treatment of protein relating neurodegenerative diseases such as, but not limited to, Alzheimer's Disease (AD) at early stage. In addition, the nanoparticles of the present invention are found to be capable of effectively penetrating the blood brain barrier, as well as targeting the amyloid aggregates in brain tissues with shorter scanning and imaging time (i.e. of about 40 minutes) under the MRI. This is in contrast to, and thus demonstrates an improvement to, the traditional MRI methods which may usually require over 60 minutes of scanning and imaging in order to produce the same quality of images. The present invention thus offers higher sensitivity when compared with the traditional MRI methods.
In summary, the present invention provides a contrasting and/or diagnostic agent for use in medical imaging such as, but is not limited to, Magnetic Resonance Imaging (MRI) in identifying, showing, determining, or more specifically, allowing or enhancing visibility of one or more proteins or protein molecules relating to one or more neurodegenerative diseases or conditions such as, but not limited to, Alzheimer's Disease (AD). Particularly, the present invention provides a composition comprising metal ion-metal ion chelating agent complex nanoparticles identifiable under the MRI. In one preferred embodiment, the composition comprises curcumin-iron metal ion complex nanoparticles capable of binding with one or more of an aggregate, plaque, tissue, deposit, fibril, oligomer and/or molecule of beta amyloid (Aβ) proteins.
Specifically, in contrast to the prior technologies which allow tagging or labelling of beta amyloid (Aβ) plaques in brain tissues and thus revealing the affected regions under the MRI at low sensitivity and spatial resolution, the present invention provides metal ion complex nanoparticles of sufficiently small size which enable binding of the nanoparticles to not only the relatively large targets of the beta amyloid (Aβ) plaques, but also beta amyloid (Aβ) fibrils and/or oligomers, which are typically too small to be effectively traceable and/or taggable in practice. The present invention therefore offers a significant improvement to the current technology to allow the screening and/or diagnostic of AD at a much earlier stage. The present invention also provides a relatively simple and inexpensive method in preparing the metal ion complex nanoparticles, with high reproducibility achievable. The prepared nanoparticles also demonstrate superior stability, for example, with the PVP stabilized core-shell nanoparticles formed via the T-joint mixing device showed no signs of precipitation after being stored in room condition for more than six months. The present description illustrates the principles of the present invention. It will thus be appreciated that those skilled in the art will be able to devise various arrangements that, although not explicitly described or shown herein, embody the principles of the invention and are included within its spirit and scope.
Moreover, all statements herein reciting principles, aspects, and embodiments of the invention, as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents as well as equivalents developed in the future, i.e., any elements developed that perform the same function, regardless of structure.
While the invention has been illustrated and described in detail in the drawings and foregoing description, the same is to be considered as illustrative and not restrictive in character, it being understood that only exemplary embodiments have been shown and described and do not limit the scope of the invention in any manner. It can be appreciated that any of the features described herein may be used with any embodiment. The illustrative embodiments are not exclusive of each other or of other embodiments not recited herein. Accordingly, the invention also provides embodiments that comprise combinations of one or more of the illustrative embodiments described above. Modifications and variations of the invention as herein set forth can be made without departing from the spirit and scope thereof, and, therefore, only such limitations should be imposed as are indicated by the appended claims.
In the claims hereof, any element expressed as a means for performing a specified function is intended to encompass any way of performing that function. The invention as defined by such claims resides in the fact that the functionalities provided by the various recited means are combined and brought together in the manner which the claims call for. It is thus regarded that any means that can provide those functionalities are equivalent to those shown herein.
In the claims which follow and in the preceding description of the invention, except where the context requires otherwise due to express language or necessary implication, the word “comprise” or variations such as “comprises” or “comprising” is used in an inclusive sense, i.e. to specify the presence of the stated features but not to preclude the presence or addition of further features in various embodiments of the invention.
It is to be understood that, if any prior art publication is referred to herein, such reference does not constitute an admission that the publication forms a part of the common general knowledge in the art.
