Patent Application
20100248297
This application claims priority of Taiwan Patent Application No. 098110627, filed on Mar. 31, 2009, the entirety of which is incorporated by reference herein.
1. Field of the Invention
The invention relates to a class of methods to prepare particles down to the size of nanometers or less in colloidal solutions, to the products obtained with such methods and to the ingredients used by such methods. The preparation of particles is based on the irradiation of a precursor-containing solution by a beam of high-energy photons (x-rays or gamma rays), or of neutrons, or of protons or of electrons.
2. Description of the Related Art
Colloidal nanoparticles are utilized as active elements in biosensor, bio-imaging and tumor treatment biomedical applications. Examples of excellent biocompatibility and highly selective agglomeration in cancer cells and tissues of colloidal nanoparticles in medicine applications have been disclosed (Langmuir 21 (2005) 10644; Durg Deliv. 11 (2004) 169; Curr. Opin. Biotechnol. 11 (2000) 215).
For biomedical applications of colloidal nanoparticles, chemical reduction methods are used to obtain suitable sizes, sufficient colloidal stability and appropriate surface properties for biocompatibility and favorable biological response. Unfortunately, the size of the particles synthesized by chemical reduction methods is normally larger than 30 nm (Durg Deliv. 11 (2004) 169; J. Controlled Release 111 (2006) 382; Macromol. Biosci. 6 (2006) 506).
To obtain smaller particles, some kinds of templates are usually utilized such as porous silica, micelle, and emulsion. However, a tedious procedure to remove the template materials results in serious problem in particle purification. In addition, due to the very low concentration of particles, about 0.25 mM, nanosols must be condensed to reach higher particle concentrations by high speed centrifugation.
To solve these problems, an emitted light without a reducing agent has been disclosed to synthesize particles (New J. Chem. 22 (1998) 1239; New J. Chem. 22 (1998) 1257). However, if a low dose rate of γ-rays (A typical γ-rays source has a dose rate up to 5 Gy per hou) is used, the reactions might need to proceed for several hours, which is not favorable for nanoparticle formation. Additionally, manufacturing yields are very low.
Jpn. J. Appl. Phys. 42 (2003 14240) discloses a method for manufacturing particles using microwave heating. Langmuir 21 (2005 437) disclose a method for manufacturing particles using a conventional X-ray source. However, a hydrophilic polymer must be used as a stabilizer in these methods to prevent the aggregation of particles. Further, due to the low reaction rate, exposure time in excess of 30 hours is needed. Since colloid solutions are thermodynamically instable, prolonged exposure of formed nanoparticles to X-ray radiation is unfavorable and results in temperature increase that eventually destroys colloidal stability.
Thus, a novel method for manufacturing particles is required to circumvent the previously mentioned problems.
The invention provides a method for preparing particles, comprising: providing a precursor solution containing a precursor dissolved in a solution; and irradiating the precursor solution with an ionizing radiation beam with high energy and high flux to convert the precursor to the particle.
The invention further provides a particle prepared by the above method.
A detailed description is given in the following embodiments with reference to the accompanying drawings.
The present invention can be more fully understood by reading the subsequent detailed description and examples with references made to the accompanying drawings, wherein:
a-1b show particles of the invention formed on a surface of a granular structure;
a-3j show TEM images and corresponding size histograms of Ag particles;
a-6d show images of direct observation of Fe3O4 particles obtained at different concentrations of dextran;
a-9d show TEM images of Au/TiO2 particles;
a-11b show TEM images of Au/ZnO and Au/ZrO2 particles; and
The following description is of the best-contemplated mode of carrying out the invention. This description is made for the purpose of illustrating the general principles of the invention and should not be taken in a limiting sense. The scope of the invention is best determined by reference to the appended claims.
The invention provides a method for synthesizing and preparing particles. The method comprises providing a precursor solution containing a precursor dissolved in a solution, and irradiating the precursor solution with an ionizing radiation beam with high energy and high flux to convert the precursor to the particle.
First, a precursor is dissolved in a solution to form a precursor solution, and the precursor solution is reacted by an ionizing radiation beam.
The term “precursor”, as used herein, refers to any material which is able to form particles. The precursor of the invention includes, but is not limited to, AgNO3, HAuCl4, FeCl2.4H2O, NiSO4−, CuSO4−, or ZnCl4 etc. In one embodiment, in order to form silver particles, AgNO3 can be used as a precursor. In another embodiment, in order to form iron particles, FeCl2.4H2O can be used as a precursor. The concentration of the precursor is not limited. Generally, the concentration of the precursor is about 0.5 to 2 mM.
The term “solution”, as used herein, refers to water, de-ionized water, or alcohol (methanol, ethanol, propyl alcohol, and butanol), but is not limited thereto. One of ordinary skill in the art will select other appropriate solutions, such as, carbon tetrachloride or chloroform.
Next, the precursor solution is exposed by a high energy and high flux radiation beam to convert the precursor to a particle.
The term “high energy and high flux radiation”, as used herein, refers to a radiation with a dose rate of more than 0.3 J/cm2sec. It is noted that if the dose rate is smaller than 0.3 J/cm2sec, the particle may not be synthesized. Under the irradiation conditions, a large amount of free radicals in the solution which are exposed by the ionizing radiation beam are quickly produced in a short amount of time, wherein the free radicals simultaneously react with all precursors. Thus, the high energy and high flux radiation prevents the aggregation of particles caused by long time exposure of low energy radiation. Examples of radiation include, but are not limited to, X-ray, neutron beam, electron beam, or ion beam. The radiation of the invention includes any radiation which is capable of producing a large number of free radicals for the above mentioned chemical reaction.
The exposure time for the high energy and high flux radiation may be less than 30 minutes, preferably, less than 30 seconds. In one embodiment, the exposure time can be one second. The exposure time of high energy and high flux radiation corresponds to the volume of the precursor solution. The exposure time is increased depending on increasing the volume of the precursor solution. In one embodiment, if the volume of the precursor solution is 10 ml, the exposure time of the high energy and high flux radiation may more than 5 minutes.
Further, before irradiating with the high energy and high flux radiation, poly(ethylene glycol) (PEG), poly(etherimide) (PEI), polyvinyl pyrrolidone (PVP), or isopropylalcohol (IPA) can be added to the precursor solution to increase the stability of the particles. The concentration ratio of PEG, PEI, PVP, or IPA to precursor may be about 0.0001:0.12. The molecular weight of PEG may be between 1000 and 250000.
Moreover, to achieve the best irradiation conditions, the pH level of the precursor solution can be adjusted to 4 to 10 by an NaOH or HCl solution.
In one embodiment, the precursor solution further includes a granular structure. The granular structure of the invention can be a metal or metal oxide, but is not limited thereto. Examples of the granular structure include phosphorous, gold, silver, titanium oxide, zinc oxide, or zirconium oxide. The granular structure can be a commercial product, or be formed by the method for synthesizing particles of the invention.
The particle would be formed on the surface of the granular structure, if the precursor solution contains a granular structure. Referring to
Note that it is important for the suitable irradiation intensity. The irradiation intensity of the invention clearly differentiates the present invention with respect to each and all previous investigations, and it is essential for the products quality and therefore for their commercial value with respect to the production costs. The irradiation intensity is specified in terms of dosing rate.
The product (particle) quality refers to the high colloidal density, to the high colloidal stability, and to the chemical purity of the colloidal solution. The commercial value refers, but is not limited, to applications of the products for medical diagnosis and therapy.
The irradiation intensity is a crucial element of the invention for several reasons. First, it increases the production yield thus reducing the production costs. More fundamentally, the methods of the invention are based on the production of free radicals in solution that are unstable; without a suitable irradiation intensity, the production would be simply impossible and not just less expensive. The high intensity is also required for phenomena like the particle charging, underlying the achievement of high colloidal density. Finally, high intensity and therefore fast production avoids significant evolution in time of the solution parameters, to the benefit of uniform particle size and other product characteristics.
In addition to high intensity, the invented methods also require high-energy irradiation allowing production in the entire volume of the precursor-containing solution. For example, the methods can use high-energy photons (x-rays or gamma rays) but not visible photons.
The value of the invention resides on the fact that products with similar quality cannot at present be obtained with other methods. This refers, but is not limited, to the coating of voids inside particles.
In addition to the product quality (stability, concentration and purity), the method of the invention also provides the benefits. The benefits includes: (1) the absence of any pre-added reducing agent or surfactant so that the fabrication process inherently clean and simple for large scale production; (2) the methods do not require templates for particle size control, which simplifies the process; (3) the high intensity irradiation automatically performs sanitization of the products; (4) the irradiation-induced synthesis allows patterning with high precision; (5) the high intensity irradiation induces the required processes for the fabrication in very short exposure times, allowing high production yields and decreasing the costs; (6) the methods allow accurate control of the product characteristics including, but not limited to, the particle size; (7) the methods use water solutions, more biocompatible, simpler to use and less expensive than solutions in oil or other solvents; (8) the chemical reactions can be complete so that chemical purity is obtained without additional processing; (9) the production occurs at room temperature or low temperature, reducing the fabrication costs and avoiding high-temperature damage of the ingredients and products; (10) the entire procedure takes place in only one container even for complex products such as coated particles; (11) the products include not only particles with coated outside surfaces but also particles with controlled filling of the internal voids; (12) the product characteristics are uniform; this specifically refers, but is not limited, to the particle size distribution and to the particle coating, and (13) the methods can be applied to complex chemical reactions to obtain products with versatile characteristics.
In contrast to the conventional method, the method of the invention provides advantages including: (a) having a one-pot reaction without a reducing agent; (b) a pure reactant and product with no need for a surfactant and product side-product; (c) no free radical scavenger, such as 2-propyl alcohol, is required; (d) a short reaction time at room temperature; (e) a colloidal containing particles with high concentrations by large scale manufacturing process; (f) maintenance of superior dispersion, shape, and size of the particles, with recovery rate of about 80%, due to easy concentration of the particles by a centrifugation at 4000. In contrast, the particles synthesized by the conventional method have to be concentrated by a centrifugation at 10000 rpm; (g) superior in vivo stability of the particles; and (h) applicability to synthesize particles from various materials.
Further, the invention also provides a particle colloidal, comprising a solution and a plurality of particles dispersed in the solution. The particle colloidal may not contain a surfactant or stabilizer.
The particle colloidal can be concentrated by a centrifugation to obtain a concentrated liquid, and the concentrated liquid can be further diluted to a solution. In the repeated concentration and dilatation process, the size of the particles is consistent.
The material of the particle of the invention is not limited. In one embodiment, the material of the invention may be a metal, such as gold, silver, nickel, platinum, iron, titanium, zinc, zirconium, or tungsten. In another embodiment, the material of the invention may be a metal oxide, such as iron oxide, nickel oxide, silicon oxide, zinc oxide, zirconium oxide, cerium oxide, tungsten oxide, titanium oxide, or copper oxide,
The surface of the particle of the invention can be coated with PEG, PEI, PVP, or IPA, etc.
The particle of the invention can be formed on a surface of a granular structure or a substrate. The granular structure or substrate can be a metal or metal oxide, but is not limited thereto. For example, the particle can be completely or partially formed on the surface of the granular structure. In another embodiment, the granular structure has internal voids, and the particle is partially or completely formed in internal voids of the granular structure. The granular structure with internal voids may be a porous silicon oxide or carbon nanotube.
The particle of the invention can be a nanoparticle. The size of the particle of the invention is not limited, preferably, about 15±5 nm, and the particle of the invention has superior dispersion, shape, and size.
Silver (Ag) particles were synthesized from aqueous silver nitrate solutions (AgNO3, 0.1 N, Acros Organics Inc. NJ US.) by synchrotron x-ray irradiation. Silver nitrate was dissolved in de-ionized water (18.2 MΩcm, Millipore, Milli-Q, MA, U.S.), and the pH value of the silver nitrate solution was adjusted using a NaOH solution to achieve desired irradiation conditions. 0.5, 1, or 2 mM of silver nitrate solution was added to the polypropylene conical tubes of different sizes (2, 15 ml, Falcon®, Becton Dickinson, N.J. US), and then exposed to a Bl01A beam line. The experiments were performed at the BL01A beam line of the NSRRC (National Synchrotron Radiation Research Center) storage rings (Margaritondo G, Hwu Y and Je J H 2004 Rivista del Nuovo Cimento 27 7). The photons energy distribution was centered between 10 and 15 keV and the dose rate was 5.1±0.9 kGy/sec as determined by a Fricke dosimeter with an estimated G value of 13. Detailed descriptions of the experimental system have been described in Kim C C, Wang C H, Yang Y C, Hwu Y, Seol S K, Kwon Y B, Chen C H, Liou H W, Lin H M, Margaritondo G and Je J H 2006 Mater Chem. Phys. 100 292; Yang Y C, Wang C H, Hwu and Y, Je J H Mater Chem. Phys. 100 72; Wang C H, Hua T E, Chien C C, Yu Y L, Yang T Y, Liu C J, Leng W H, Hwu Y, Yang Y C, Kim C C, Je J H, Chen C H, Lin H M and Margaritondo G. 2007 Mater Chem. Phys. 106 323; and Wang C H, Chien C H, Yu Y L, Liu C J, Lee C F, Chen C H, Hwu Y, Yang C H, Je J H and Margaritondo G. 2007 J. Synchrotron Radiation 14 477. Unmonochromatized (“pink”) x-ray beams with no optical elements except one set of beryllium and Kepton windows was used in this Example. A slit system was used to obtain a transversal beam section of 13×10 mm2. The exposure time ranged from 1 sec to 3 min.
Before irradiation of X ray, PEG (MW 6000, Showa Inc., Japan), PVP (MW 10000, Tokyo Chemical Industry Co., Ltd. Japan) or isopropanol (Mallinckrodt Baker Inc. NJ, US) was added to the silver nitrate solution to improve colloidal stability, size distribution, and biocompatibility. In addition, conventional citrate-reduced silver nanoparticles (citrate-Ag) were also prepared as a control group.
The resulting Ag nanosols of the invention were determined to have been stable for over one year in an ambient environment. On the contrary, the reference silver nanoparticle solutions prepared by citrate reduction (and no irradiation) tended to flocculate and precipitate before one year.
Referring to
The influence of the irradiation time on the structure and optical absorption spectra of Ag particles prepared by Example 1 was investigated.
i to 3j show the size histograms of Au particles synthesized from hydrogen tetrachloroaurate solution (HAuCl4.3H2O) by the method of Example 1. After 1 second exposure of X-ray, the size of the gold particle was smaller than 10 nm. The results indicate that particles of the invention were stably synthesized by a short 1 second exposure time.
The Ni—P colloidal was also well synthesized by the method of Example 1. The Ni base structure could be applied in diverse fields such as the electronics, medical, aerospace, automobile, oil, and gas industries since they exhibit excellent resistance to corrosion, oxidation, and wear.
The plating bath consisted of 26 g 1−1 nickel sulfate, 30 g 1−1 sodium hypophosphite, 20 g 1−1 ammonium chloride, and 30 g 1−1 sodium acetate. Sodium hypophosphite was used as a reduction agent and sodium acetate was used as a complexing agent. The pH level of the solution was adjusted to 4, 5, 6, 7, and 8 by diluted HCl and NaOH solutions, respectively.
Iron oxide (Fe3O4 ferrifluid, SDCIO) particles were synthesized from aqueous iron chloride (FeCl2.4H2O) solutions by the method of Example 1. To achieve the best irradiation conditions, the pH level of the iron chloride solution was adjusted by adding an NH4OH solution. The exposure time was set at 5 minutes for each 10 ml of the iron chloride solution.
In addition, to improve the colloidal stability of Fe3O4 colloidal, dextran was added to the iron chloride solution before synchrotron X-ray irradiation. Direction observations of Fe3O4 nanosols obtained from the iron chloride solutions on different dextran concentrations are shown in
Referring to
The photocatalytic efficiency of specific metal oxide, such as TiO2, ZnO, or ZrO2 is an important performance factor for practical applications in areas such as solar cells, sanitization and cancer therapy. Thus, efficiency can be improved by adding Au to a surface thereof. Gold (Au) is particularly interesting because of its biocompatibility and its enhancements of the radiation response. The surfaces of both oxide and of the metal particles must be optimized to achieve maximum efficiency.
0.01 g of commercial P25 TiO2 (anatase phase, specific surface area 50±15 m2/g, average primary particle size 21±3 nm, purity>99.5%) were added to 10 ml of de-ionized water to prepare a well mixed TiO2 nanosol. A mixed aqueous solution containing 0.02 M of gold precursor (hydrogen tetrachloroaurate trihydrate, HAuCl4.3H2O, Aldrich) with an appropriate amount of NaOH (0.1 M) was subsequently added while stirring. The resulting solution was then exposed to high flux of X-ray photons at the BL01A beam line to form the Au/TiO2 particles. The synthesis of Au/TiO2 particles was the same as Example 1.
Referring to
Further, TEM analysis was also used to confirm the size distribution and morphology of the Au particles.
The hydrodynamic size of TiO2 and Au/TiO2 nanosols in the RPMI medium or de-ionized water was monitored during 4 hours by dynamic light scattering and the results are summarized in Table 1.
For suspensions in distilled water, the initial average hydrodynamic size of the Au/TiO2 nanosol was 225.3 nm, which is almost the same as that of the TiO2 nanosol (239.1 nm). However, while the TiO2 particles incubated for up to 4 hours, their average hydrodynamic size increased to approximately 342.5 nm. On the contrary, no such increase was observed for Au/TiO2 nanoparticles with average sizes almost unchanged (246.2 nm).
For suspensions in cell culture medium, the initial average hydrodynamic size of TiO2 and Au/TiO2 particles (487.2 and 318.7) was large compared to distilled water. During incubation for up to 4 hours, the TiO2 particle size increased significantly to 635.1 nm, whereas the growth was more limited for Au/TiO2 particles (341 nm). All the results indicated that the colloidal stability of Au/TiO2 nanosol was much better than pure TiO2 nanosol both in distilled water and in the cell culture medium. The size increase of particles in the cell culture medium may be explained by protein absorption.
Treatment condition: MB=0.25 mM; TiO2=0.5 mM; Au/TiO2=0.028 mM/0.5 mM
Au/TiO2, Au/ZnO and AuZrO2 were synthesized by synchrotron X-ray irradiation as mentioned in Example 5.
In Comparative Example 1, the X-Ray exposure experiments were conducted at two additional X-Ray sources: (1) in house X-Ray diffractometer (Photon energy of 8 KeV); and (2) Synchrotron X-Ray at BL 17C1 with a photon energy of 11.919 KeV (matching Au L3 edge). The criteria to judge whether the gold ions had been reduced to zero-valence gold was by examining the color change of the solution after exposure. The hydrogen tetrachloroaurate solution with the same composition as for white synchrotron X-Rays were contained either in a plastic plate (For in house X-Ray machine) or capsulated within a plastic bag (for Synchrotron X-Ray). After 2-3 hours of exposure, the solution color didn't change. The results indicated that both X-Rays sources are incapable for producing gold particles in solutions due to their low intensity of X-Rays.
While the invention has been described by way of example and in terms of the preferred embodiments, it is to be understood that the invention is not limited to the disclosed embodiments. To the contrary, it is intended to cover various modifications and similar arrangements (as would be apparent to those skilled in the art). Therefore, the scope of the appended claims should be accorded the broadest interpretation so as to encompass all such modifications and similar arrangements.
| Number | Date | Country | Kind |
|---|---|---|---|
| 098110627 | Mar 2009 | TW | national |
