Claims
- 1. A method of separating a preselected biological molecule from a sample containing same comprising:
- (a) loading a column with a sample containing a preselected biological molecule to be separated from a mixture, wherein said column is packed with the passivated chromatographic media comprising (i) a porous substrate matrix having interior and exterior surfaces and innate groups that render said substrate matrix susceptible to undesirable non-specific interaction with one or more biological molecules, and (ii) a three-dimensional, pore-filling gel network derived from polymerization of a main monomer, wherein said main monomer is selected to provide a polymer network that has an affinity for a preselected biological molecule; a passivating agent comprising polyethyleneimine; and a crosslinking agent; and wherein said gel network extends into and throughout the porous volume of said substrate matrix to substantially completely fill said porous volume, and wherein said groups of said matrix become deactivated, resulting in the substantial elimination of said undesirable non-specific interaction;
- (b) passing an eluent solution through said loaded column to effect the separation of said biological molecule.
- 2. The method of claim 1, wherein said biological molecule is a protein.
- 3. A chromatographic method for the separation of biological molecules comprising passing a sample containing a mixture of biological molecules through a column containing the passivated chromatographic media comprising (i) a porous substrate matrix having interior and exterior surfaces and innate groups that render said substrate matrix susceptible to undesirable non-specific interaction with one or more biological molecules, and (ii) a three-dimensional, pore-filling gel network derived from polymerization of a main monomer, a passivating agent comprising polyethyleneimine, and a crosslinking agent, and wherein said gel network extends into and throughout the porous volume of said substrate matrix to substantially completely fill said porous volume, and wherein said groups of said matrix become deactivated, resulting in the substantial elimination of said undesirable non-specific interaction.
- 4. The chromatographic method of claim 3, wherein said column is selected from the group consisting of a packed column and a fluidized-bed column.
1. CROSS-REFERENCE TO RELATED APPLICATIONS
This application is a divisional application of application Ser. No. 08/858,924, filed on May 19, 1997, now U.S. Pat. No. 5,906,734, which is a continuation-in-part of application Ser. No. 08/394,014 filed on Feb. 22, 1995, now abandoned, which is a continuation-in-part of application Ser. No. 08/254,625, filed Jun. 6, 1994, now U.S. Pat. No. 5,445,732, which is a continuation of application Ser. No. 07/955,935, filed Oct. 5, 1992, now abandoned, which is a continuation-in-part of application Ser. No. 07/901,326, filed Jun. 19, 1992, now abandoned.
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