Claims
- 1. A compound having the carbocyanine dye bioconjugate of formula 2
- 2. The compound of claim 1 wherein W1 and W2 are NR12; Y1 and Y2 are selected from the group consisting of hydrogen, tumor-specific agent, —CONH-Bm, —NHCO-Bm, —(CH2)a—CONH-Bm, —CH2—(CH2OCH2)b—CH2—CONH-Bm, —(CH2)a—NHCO-Bm, —CH2—(CH2OCH2)b—CH2—NHCO-Bm, —(CH2)a—NR12R13, and —CH2(CH2OCH2)b—CH2NR12R13; Z1 and Z2 are independently selected from the group consisting of hydrogen, phototherapy agent, —CONH-Dm, —NHCO-Dm, —(CH2)a—CONH-Dm, —CH2—(CH2OCH2)b—CH2—CONH-Dm, —(CH2)a—NHCO-Dm, —CH2—(CH2OCH2)b—CH2—NHCO-Dm, —(CH2)a—N R12R13, and —CH2(CH2OCH2)b—CH2N R12R13; K1 and K2 are independently selected from the group consisting of C1-C10 alkyl, C5-C20 aryl, C1-C20 alkoxyl, C1-C20 aminoalkyl, —(CH2)a—CO—, —(CH2)a—CONH, —CH2—(CH2OCH2)b—CH2—CONH—, —(CH2)a—NHCO—, —CH2—(CH2OCH2)b—CH2—NHCO—, and —CH2—(CH2OCH2)b—CO—; X1 and X2 are single bonds, or are independently selected from the group consisting of nitrogen, —CR14—, —CR14R15, and —NR16R17; Q is a single bond or is selected from the group consisting of —O—, —S—, and —NR18; a1 and b1 independently vary from 0 to 3; Bm is selected from the group consisting of bioactive peptide containing 2 to 30 amino acid units, protein, antibody fragment, mono- and oligosaccharide; Dm is selected from the group consisting of photosensitizer, photoactive molecule, and phototherapy agent; a and c independently vary from 1 to 10; b and d independently vary from 1 to 30.
- 3. The compound of claim 2 wherein each K1 and K2 is —(CH2)4CO—; each Q, X1 and X2 is a single bond; each R19 to R31, Y1 and Z1 is H; Y2 is a tumor-specific agent; and Z2 is a phototherapy agent.
- 4. The compound according to claim 3 wherein the said tumor-specific agent is a bioactive peptide containing 2 to 30 amino acid units.
- 5. The compound according to claim 4 wherein the said tumor-specific agent is octreotate and bombesin (7-14).
- 6. The compound according to claim 3 wherein the said phototherapy agent is a photosensitizer.
- 7. The compound according to claim 6 wherein the said photosensitizer is 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a.
- 8. A method for performing a diagnostic and therapeutic procedure comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate of Formula 2
- 9. The method for performing the diagnostic and therapeutic procedure of claim 8 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein W1 and W2 are NR12; Y1 and Y2 are selected from the group consisting of hydrogen, tumor-specific agent, —CONH-Bm, —NHCO-Bm, —(CH2)a—CONH-Bm, —CH2—(CH2OCH2)b—CH2—CONH-Bm, —(CH2)a—NHCO-Bm, —CH2—(CH2OCH2)b—CH2—NHCO-Bm, —(CH2)a—NR12R13, and —CH2(CH2OCH2)b—CH2NR12R13; Z1 and Z2 are independently selected from the group consisting of hydrogen, phototherapy agent, —CONH—Dm, —NHCO-Dm, —(CH2)a—CONH-Dm, —CH2—(CH2OCH2)b—CH2—CONH-Dm, —(CH2)a—NHCO-Dm, —CH2—(CH2OCH2)b—CH2—NHCO-Dm, —(CH2)a—N R12R13, and —CH2(CH2OCH2)b—CH2N R12R13; K1 and K2 are independently selected from the group consisting of C1-C10 alkyl, C5-C20 aryl, C1-C20 alkoxyl, C1-C20 aminoalkyl, —(CH2)a—CO—, —(CH2)a—CONH—, —CH2—(CH2OCH2)b—CH2—CONH—, —(CH2)a—NHCO—, —CH2—(CH2OCH2)b—CH2—NHCO—, and —CH2—(CH2OCH2)b—CO—; X1 and X2 are single bonds, or are independently selected from the group consisting of nitrogen, —CR14—, —CR14R15, and —NR16R17; Q is a single bond or is selected from the group consisting of —O—, —S—, and —NR18; a1 and b1 independently vary from 0 to 3; Bm is selected from the group consisting of bioactive peptide containing 2 to 30 amino acid units, protein, antibody fragment, mono- and oligosaccharide; Dm is selected from the group consisting of photosensitizer, photoactive molecule, and phototherapy agent; a and c independently vary from 1 to 10; b and d independently vary from 1 to 30.
- 10. The method for performing the diagnostic and therapeutic procedure of claim 9 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein each K1 and K2 is —(CH2)4CO—; each Q, X1 and X2 is a single bond; each R19 to R31, Y1 and Z1 is H; Y2 is a tumor-specific agent; and Z2 is a phototherapy agent.
- 11. The method for performing the diagnostic and therapeutic procedure of claim 10 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein the said tumor-specific agent is a bioactive peptide containing 2 to 30 amino acid units.
- 12. The method for performing the diagnostic and therapeutic procedure of claim 11 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein the said tumor-specific agent is octreotate and bombesin (7-14).
- 13. The method for performing the diagnostic and therapeutic procedure of claim 10 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein the said phototherapy agent is a photosensitizer.
- 14. The method for performing the diagnostic and therapeutic procedure of claim 13 comprising administering to an individual an effective amount of the composition of cyanine dye bioconjugate wherein the said photosensitizer is 2-[1-hexyloxyethyl]-2-devinylpyropheophorbide-a.
- 15. The method of claim 8 wherein said procedure utilizes light of wavelength in the region of 300-1300 nm.
- 16. The method of claim 8 wherein the diagnostic procedure is optical tomography.
- 17. The method of claim 8 wherein said diagnostic procedure is fluorescence endoscopy.
- 18. The method of claim 8 wherein said procedure further comprises a step of imaging and therapy wherein said imaging and therapy is selected from the group consisting of absorption, light scattering, photoacoustic and sonofluoresence technique.
- 19. The method of claim 8 wherein said procedure is for diagnosing and treating atherosclerotic plaques and blood clots.
- 20. The method of claim 8 wherein said procedure comprises administering localized therapy.
- 21. The method of claim 8 wherein said therapeutic procedure comprises photodynamic therapy.
- 22. The method of claim 8 wherein said therapeutic procedure comprises laser assisted guided surgery (LAGS) for the detection and treatment of micrometastases.
Parent Case Info
[0001] This application is a Divisional of U.S. patent application Ser. No. 09/978,725 filed Oct. 17, 2001, now pending and expressly incorporated by reference herein in its entirety.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09978725 |
Oct 2001 |
US |
Child |
10847121 |
May 2004 |
US |