Claims
- 1. A microfluidic device for performing PCR and nucleic acid separations, the device comprising at least one microscale channel and a sieving medium, which sieving medium is disposed within the at least one microscale channel and comprises a polymer solution, which polymer solution comprises less than about 0.5% polymer.
- 2. The microfluidic device of claim 1, wherein the polymer solution comprises less than about 0.4% polymer.
- 3. The microfluidic device of claim 1, wherein the polymer solution comprises about 0.35% polymer or less.
- 4. The microfluidic device of claim 1, wherein the polymer solution comprises acrylamide.
- 5. The microfluidic device of claim 4, wherein the acrylamide comprises linear acrylamide, polyacrylamide, polydimethylacrylamide, or polydimethylacrylamide/coacrylic acid.
- 6. The microfluidic device of claim 1, wherein the polymer solution comprises agarose, methyl cellulose, polyethylene oxide, hydroxycellulose, or hydroxy ethyl cellulose.
- 7. The microfluidic device of claim 1, further comprising one or more proteins, nucleic acids, PCR reaction components, or PCR products disposed within the at least one microfluidic channel.
- 8. The microfluidic device of claim 7, wherein the PCR reaction components comprise one or more of: a thermostable DNA polymerase, a plurality of nucleotides, a nucleic acid template, a primer which hybridizes to the nucleic acid template, or Mg++.
- 9. A method of separating polynucleotides, the method comprising:
(i) providing two or more polynucleotides; (ii) providing a sieving medium, which sieving medium comprises a polymer solution, which polymer solution comprises less than about 0.5% polymer; (iii) allowing the two or more polynucleotides to migrate through the sieving medium, thereby separating the two or more polynucleotides.
- 10. The method of claim 9, wherein the polynucleotides comprise one or more PCR products, RNA, or DNA.
- 11. The method of claim 9, wherein the polymer solution comprises less than about 0.4% polymer.
- 12. The method of claim 9, wherein the polymer solution comprises about 0.35% polymer or less.
- 13. The method of claim 9, wherein the polymer solution comprises acrylamide.
- 14. The method of claim 13, wherein the acrylamide comprises linear acrylamide, polyacrylamide, polydimethylacrylamide, or polydimethylacrylamide/coacrylic acid.
- 15. The method of claim 9, wherein the polymer solution comprises agarose, methyl cellulose, polyethylene oxide, hydroxycellulose, or hydroxy ethyl cellulose.
- 16. The method of claim 9, further comprising introducing the sieving medium into a microfluidic channel and allowing the two or more polynucleotides to migrate through the sieving medium in the microfluidic channel.
- 17. The method of claim 9, wherein the separating comprises electrophoretically separating the two or more polynucleotides.
- 18. A method of performing PCR and separating one or more PCR products, the method comprising:
(i) mixing one or more PCR reaction components with a sieving medium to provide a PCR sieving medium, wherein the sieving medium comprises a polymer solution, which polymer solution comprises less than about 0.5% polymer; and (ii) thermocycling the PCR sieving medium to produce one or more PCR products; and, (iii) separating the one or more PCR products by flowing the one or more PCR products through the sieving medium.
- 19. The method of claim 18, wherein the polymer solution comprises less than about 0.4% polymer.
- 20. The method of claim 19, wherein the polymer solution comprises about 0.35% polymer or less.
- 21. The method of claim 18, wherein the polymer solution comprises acrylamide.
- 22. The method of claim 21, wherein the acrylamide comprises linear acrylamide, polyacrylamide, polydimethylacrylamide, or polydimethylacrylamide/coacrylic acid.
- 23. The method of claim 28, wherein the polymer solution comprises agarose, methyl cellulose, polyethylene oxide, hydroxycellulose, or hydroxy ethyl cellulose.
- 24. The method of claim 18, wherein the one or more PCR reaction components comprise one or more of: a thermostable DNA polymerase, a plurality of nucleotides, a nucleic acid template, a primer which hybridizes to the nucleic acid template, or Mg++.
- 25. The method of claim 18, comprising mixing the PCR reaction components with the sieving medium in a microfluidic channel.
- 26. The method of claim 25, further comprising separating the one or more PCR products by flowing the one or more PCR products through the sieving medium in the microfluidic channel.
- 27. The method of claim 26, wherein separating comprises electrophoretically separating.
- 28. A nucleic acid sieving medium, which medium comprises one or more polynucleotides, one or more PCR reagents, and a polymer solution, which polymer solution comprises less than about 0.5% polymer.
- 29. The sieving medium of claim 28, wherein the polymer solution comprises less than about 0.4% polymer.
- 30. The sieving medium of claim 29, wherein the polymer solution comprises about 0.35% polymer or less.
- 31. The sieving medium of claim 28, wherein the polymer solution comprises acrylamide.
- 32. The sieving medium of claim 31, wherein the acrylamide comprises linear acrylamide, polyacrylamide, polydimethylacrylamide, or polydimethylacrylamide/coacrylic acid.
- 33. The sieving medium of claim 28, wherein the polymer solution comprises agarose, methyl cellulose, polyethylene oxide, hydroxycellulose, or hydroxy ethyl cellulose.
- 34. The sieving medium of claim 28, wherein the one or more PCR reaction components comprise one or more of: a thermostable DNA polymerase, a plurality of nucleotides, a nucleic acid template, a primer which hybridizes to the nucleic acid template, or Mg++.
- 35. The sieving medium of claim 28, wherein the one or more polynucleotides comprise DNA, RNA, or PCR products.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] Pursuant to 35 U.S.C. § 119(e) and any other applicable statute or rule, the present application claims benefit of and priority to U.S. Ser. No. 60/190,773, entitled “PCR COMPATIBLE NUCLEIC ACID SIEVING MEDIUM,” filed Mar. 20, 2000 by Burd Mehta.
Provisional Applications (1)
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Number |
Date |
Country |
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60190773 |
Mar 2000 |
US |