Claims
- 1. A polymerase chain reaction method for amplifying a gene, comprising,
- providing a polymerase chain reaction mixture stored in a sample container,
- forming a throughhole for inserting said sample container in a heating and cooling apparatus,
- inserting said sample container in said throughhole, said sample container comprising a container made of metal having a thickness ranging from 0.02 mm to 1.0 mm, and a resin layer on the whole inner surface of the container, the resin layer having a thickness ranging from 1 .mu.m to 100 .mu.m, and
- heating and cooling said sample container.
- 2. A polymerase chain reaction method for amplifying a gene, comprising,
- providing a polymerase chain reaction mixture stored in a sample container,
- forming a throughhole for inserting said sample container in a heating and cooling apparatus,
- inserting said sample container in said throughhole, said sample container comprising a container made of metal having a thickness ranging from 0.02 mm to 1.0 mm, and a metal oxide layer on at least the whole inner surface of the container, and
- heating and cooling said sample container.
- 3. The method of claim 1, wherein a metal constituting the container has a heat conductivity of 20 W/m.multidot.k or more.
- 4. The method of claim 2, wherein a metal constituting the container has a heat conductivity of 20 W/m.multidot.k or more.
- 5. The method of claim 1, wherein said resin is selected from the group consisting of polyimide, ABS resin, polypropylene, acryl, polytetrafluoroethylene, and poly(butylene terephthalate).
- 6. The method of claim 1, wherein the resin layer has a thickness of from 1 .mu.m to 50 .mu.m.
- 7. The method of claim 1, wherein the resin layer has a thickness of from 1 .mu.m to 10 .mu.m.
- 8. The method of claim 1, wherein the container has a thickness of from 0.02 mm to 0.5 mm.
- 9. The method of claim 2, wherein the container has a thickness of from 0.02 mm to 0.5 mm.
- 10. The method of claim 1, wherein the container has a thickness of from 0.02 mm to 0.3 mm.
- 11. The method of claim 2, wherein the container has a thickness of from 0.02 mm to 0.3 mm.
- 12. The method of claim 1, wherein a metal constituting the container has a heat conductivity of 100 W/m.multidot.k or more.
- 13. The method of claim 2, wherein a metal constituting the container has a heat conductivity of 100 W/m.multidot.k or more.
- 14. The method of claim 1, wherein a metal constituting the container has a heat conductivity of 200 W/m.multidot.k or more.
- 15. The method of claim 2, wherein a metal constituting the container has a heat conductivity of 200 W/m.multidot.k or more.
- 16. The method of claim 1, wherein said metal is selected from the group consisting of nickel, molybdenum, aluminum, silver, copper and gold and alloys thereof.
- 17. The method of claim 2, wherein said metal is selected from the group consisting of nickel, molybdenum, aluminum, silver, copper and gold and alloys thereof.
Priority Claims (1)
Number |
Date |
Country |
Kind |
8-276497 |
Oct 1996 |
JPX |
|
Parent Case Info
This application is a divisional of application Ser. No. 08/951,508, filed Oct. 16, 1997.
US Referenced Citations (11)
Foreign Referenced Citations (4)
Number |
Date |
Country |
4-330272 |
Nov 1992 |
JPX |
6-86941 |
Mar 1994 |
JPX |
6-99085 |
Apr 1994 |
JPX |
WO 9401529 |
Jan 1994 |
WOX |
Divisions (1)
|
Number |
Date |
Country |
Parent |
951508 |
Oct 1997 |
|