Peptides and analogues and mixtures thereof for detecting antibodies to HTLV-I and HTLV-II viruses

TECHNICAL FIELD OF THE INVENTION
This invention relates to novel linear peptides and mixtures and chemical combinations thereof useful for detecting and quantifying HTLV-I and HTLV-II infections. These peptides are also useful in vaccines against HTLV-I and HTLV-II viral infections.
BACKGROUND OF THE INVENTION
Human T-cell leukemia virus subgroup I (HTLV-I) is a retrovirus closely associated with adult T-cell leukemia/lymphoma (ATL) (Poiesz et al., 1980, Proc. Natl. Acad. Sci. U.S.A., 77, 7415-7419). It is also linked with neurological diseases designated as tropical spastic paraparesis and HTLV-I-associated myelopathy (HAM) (Gessain et al., 1985, Lancet ii, 407-410). Human T-cell leukemia virus subgroup II (HTLV-II) was first isolated from a patient with T-cell hairy cell leukemia (Kalyanaraman et al., 1982, Science, 218, 571-573). It is associated with a T-cell variant of hairy cell leukemia.
The HTLV-I and HTLV-II genomes exist as proviruses in the human chromosomal DNA of leukemia cells. Their complete nucleotide sequences have been elucidated (Seiki et al., 1983, Proc. Natl. Acad. Sci. U.S.A., 80, 3618-3622; Shimotohno et al., 1985, Ibid., 82, 3101-3105). Like other retroviruses, the HTLV-I and HTLV-II genomes are flanked by LTR (long-terminal-repeats) structures which are believed to play an essential role in the integration of the proviral DNA into the host chromosomal DNA. Four major genes have been identified and occupy the following relative positions in the genome: LTR-gag-pol-env-pX-LTR (Seiki et al.; Shaw et al., 1984, Proc. Natl. Acad. Sci., 81, 4544-4548). The gag gene codes for a 48,000-dalton-precursor protein (often referred to as p53) consisting of 429 amino acids (433 for HTLV-II). This precursor protein is cleaved into at least three smaller proteins (FIG. 2). The pol gene codes for the viral reverse transcriptase. The env gene presumably codes for a 54,000-dalton protein which is glycosylated and later cleaved into two glycoproteins named gp46 and gp21. (Slightly different molecular weights have been reported for what are most probably the same env gene protein products). The last coding region is called pX and is believed to code for four proteins (tax.sub.1, tax.sub.2, rex.sub.1, rex.sub.2) involved in gene expression and regulation.
As depicted in FIGS. 1 and 2, the env proteins and the gag proteins of HTLV-I and of HTLV-II share a high degree of amino acid sequence homology. Furthermore, serologic cross-reactivity has been reported between the proteins of HTLV-I and those of HTLV-II (Lee et al., 1984, Proc. Natl. Acad. Sci., 81, 7579-7583). Despite this structural resemblance and cross-reactivity, some regions of these env and gag proteins are recognized only by antibodies present in a patient infected by either the HTLV-I or the HTLV-II virus.
HTLV-I and HTLV-II both differ from the human immunodeficiency viruses (HIVs) in their morphologic and genetic structures. As a result, antibodies to the HIV proteins should not cross-react with HTLV-I and HTLV-II antigens. However, some serum samples taken from patients with AIDS have shown some degree of cross-reactivity with HTLV antigens (Essex et al., 1983, Science, 220, 859-862).
Adult T-cell leukemia/lymphoma (ATL) occurs mainly in Southwestern Japan, the Caribbean basin and parts of Central and South America. In those regions, seroprevalence varies between 5% and 15% in the general population and reaches 30% in older age groups. In the United States, HTLV-I/II infections are mainly present in intravenous drug users (IVDU). In a recent American Red Cross survey (Williams et al., 1988, Science, 240, 643-646), antibodies to HTLV-I could be detected in 10 of 39,898 random blood donors in eight U.S. cities; this represents a seroprevalence rate of 0.025%. A similar study involving 3158 individuals from Northern Egypt led to the identification of two carriers (prevalence rate of 0.06%) (El-Farrash et al., 1988, Microbiol. Immunol., 32, 981-984). In these two studies, distinction between infection with HTLV-I and HTLV-II was not clearly established. These data indicate that there is a need for a reliable test to screen all blood samples destined for blood banks in order to avoid the inadvertent spread of the virus to blood product recipients.
There have been several attempts in the prior art to develop such tests. None has been successful in detecting all serum and plasma samples that are part of a well characterized commercial panel of HTLV infected fluids. Furthermore, none is able to detect HTLV infection at very low levels, thereby, ensuring safety of the blood supply and prompt and early treatment of HTLV infections.
Saxinger et al. (1984, Science, 225, 1473-1476) has reported the use of the HTLV-I particle as the immunoadsorbent in an enzyme immunoassay (EIA) for the detection of antibodies to the virus. In an improvement to this first generation test, specific HTLV antigens have been used instead of viral particles as the immunoadsorbent. For example, Samuel et al. (1984, Science, 225, 1094-1097) refers to antigens obtained by recombinant DNA technology. These detected each of 11 sera shown to contain antibodies to HTLV-I by a whole viral lysate-based EIA similar to the one developed by Saxinger et al. Slamon et al. (PCT/US85/01803) refer to assays using polypeptides and fragments thereof associated with immunogenic sites present on proteins of the pX region of HTLV-I and HTLV-II. The reported accuracy of these assays ranged between 77% and 87%. Fukui et al. (European Patent Application 87116787) refers to assays using polypeptides encoded by a fused gene comprising all or a part of the gag gene and all or a part of the env gene. They reported a sensitivity of 100% (57/57 ) with no false-positives.
Although these results look impressive, they are likely not repeatable with sera, like those most usual in the United States, which are characterized by much lower antibody titers than the Japanese sera used to validate the above assays.
Assays which use peptides derived from HTLV-I or HTLV-II proteins have also been reported. For example, Palker et al. (1989, J. Immunol., 142, 971-978) reports that antibodies in sera from 28 out of 36 patients (78%) reacted with a peptide spanning amino acids 190 to 209 of the HTLV-I envelope (peptide 4a; env 190-209).* Ten of 35 sera samples (29%) reacted with peptide 6 (env 296-312) and 6 out of 33 (18%) bound to peptide 7 (env 374-392). Palker et al. (1986, J. Immunol., 136, 2393-2397) also refers to a peptide (SP-71; gag 120-130) that reacted with 16 out of 18 HTLV-I seropositive samples.
*In this application, the amino acid sequence and numbering published by Seiki et al. (supra.) and by Shimotohno et al. (supra.) for the HTLV-I and HTLV-II gene products are used (for ease of reference only).
A peptide (SP-70; env 296-306) has been reported by Copeland et al. (1986, J. Immunol., 137, 2945-2951) to recognize antibodies from 4 out of 12 individuals seropositive for HTLV-I.
Wang et al. (U.S. Pat. No. 4,833,071) refers to three overlapping linear peptides spanning regions of the transmembrane protein (gp21) of HTLV-I. These peptides (env 381-400, env 377-400 and env 378-393), when used in a mixture, detected 102 out of 102 serum samples from patients with ATL and 5 out of 30 patients with AIDS/ARC. No immunoreactivity was found against sera from 12 normal subjects or from 12 patients with autoimmune diseases.
Reyes (PCT/W089/06543) refers to a non-glycosylated, 41-amino acid recombinant peptide antigen derived from the gp46 of HTLV-I. This recombinant peptide, env(163-203), is reported to have been used in a solid-phase assay for the determination of serum antibodies in six patients with HTLV-I infection.
Vahlne (PCT/W089/08664) refers to four synthetic peptides, A, B, C and H, spanning env(381-404), env(273-293), env(223-242) and env(176-199) of the envelope protein of HTLV-I. From the examples provided, it appears that only peptide A was useful in an ELISA for the detection of specific antibodies to HTLV-I present in the samples tested.
None of the above-described assays has demonstrated the high specificity (no false positives) and high sensitivity (detection of all positives, even when the sera contains very low levels of HTLV antibodies) necessary to ensure that HTLV infected blood products do not enter blood banks and that infected individuals seek prompt and early treatment. The peptides of this invention remedy these failures.
SUMMARY OF THE INVENTION
The peptides of this invention are selected from the group consisting of:
(i) peptides having the formula:
a-X1-b
wherein:
X1 is a sequence of at least six amino acids taken as a block from AA.sub.283 -AA.sub.309 (SEQ ID NO:5) of the gp46 env protein (HTLV-I) and analogues thereof;
a is an amino terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide; and
b is a carboxy terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide;
(ii) peptides having the formula:
a-X2-b
wherein:
X2 is a sequence of at least six amino acids taken as a block from AA.sub.273 -AA.sub.305 (SEQ ID NO:18) of the gp46 env protein (HTLV-II), and analogues thereof and a and b are as defined above;
(iii) peptides having the formula:
a-Z1-b
wherein:
Z1 is a sequence of at least six amino acids taken as a block from AA.sub.236 -AA.sub.257 (SEQ ID NO:13) of the gag p24 protein (HTLV-I), and analogues thereof and a and b are as defined above;
(iv) peptides having the formula:
a-Z2-b
wherein:
Z2 is a sequence of at least six amino acids taken as a block from AA.sub.242 -AA.sub.263 (SEQ ID NO:14) of the gag p24 protein (HTLV-II), and analogues thereof and a and b are as defined above;
(v) peptides having the formula:
a-B2-b
wherein:
B2 is a sequence of at least six amino acids taken as a block from AA.sub.171 -AA.sub.207 (SEQ ID NO:19) of the gp46 env protein (HTLV-II), and analogues thereof and a and b are as defined above; and
(vi) tandem peptides having the formula:
a-((((J).sub.n -c).sub.o -((U).sub.p).sub.q -d).sub.r).sub.s -b
wherein:
J and U are sequences of at least six amino acids taken as a block from sequences independently selected from the group consisting of AA.sub.193 -AA.sub.210 (SEQ ID NO:16) or AA.sub.283 -AA.sub.309 (SEQ ID NO:5) of the gp46 env protein (HTLV-I); AA.sub.236 -AA.sub.257 (SEQ ID NO:15) of the p24 gag protein (HTLV-I); AA.sub.120 -AA.sub.130 (SEQ ID NO:17) of the p19 gag protein (HTLV-I); AA.sub.171 -AA.sub.207 (SEQ ID NO:19) or AA.sub.273 -AA.sub.305 (SEQ ID NO:18) of the gp46 env protein (HTLV-II); AA.sub.242 -AA.sub.263 (SEQ ID NO:14) of the p24 gag protein (HTLV-II) and AA.sub.126 -AA.sub.136 (SEQ ID NO:20) of the p19 gag protein (HTLV-II); and analogues thereof;
a and b are as defined above;
c and d, if present, are independently selected from one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide;
n=1-5;
o=1-5 if c is present, otherwise o=1;
p=1-5;
q=1-5;
r=1-5 if d is present, otherwise r=1; and
s=1-5.
The peptides and mixtures thereof of this invention are useful for the screening of blood and body fluids for the presence of HTLV-I and HTLV-II infraction and in the preparation of safe, effective vaccines against HTLV-I and HTLV-II infections. For example, the peptides of this invention and mixtures thereof are useful in a wide variety of specific binding assays for the detection of antibodies to HTLV-I and HTLV-II, as immunogens for eliciting antibodies useful for the detection of HTLV-I and HTLV-II antigens and in the preparation of vaccines against HTLV-I and HTLV-II viral infections.

BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 depicts the amino acid sequence of the env gene products (gp46 and gp21) of HTLV-I (SEQ ID NO:1) and HTLV-II (SEQ ID NO:2). The amino acid residues of the sequence are given using the following single letter code: A=ala, C=cys, D=asp, E=glu, F=phe, G=gly, H=his, I=ile, K=lys, L=leu, M=met, N=asn, P=pro, Q=gln, R=arg, S=ser, T=thr, V=val, W=trp, Y=tyr. The ". . ." are spaces added to align the depicted sequences as closely as possible.
FIG. 2 depicts the amino acid sequence of the gag gene products of HTLV-I (SEQ ID NO:3) and HTLV-II (SEQ ID NO:4). Again, the ". . ." are spaces added to align the depicted sequences as closely as possible.

DESCRIPTION OF THE INVENTION
The present invention provides novel peptides and analogues thereof corresponding to immunodominant regions of the env gene and gag gene products of HTLV-I and HTLV-II. It also provides mixtures and chemical combinations of those peptides and analogues. As will be plain from the following description, these peptides, analogues, mixtures and chemical combinations are useful in a wide variety of diagnostic and preventive methods, means and compositions with respect to HTLV-I and HTLV-II and the infections caused by them. Some of the peptides, analogues, mixtures and chemical combinations of this invention are also capable of distinguishing with which virus, HTLV-I or HTLV-II, a particular patient is infected.
As set forth above, the peptides of this invention are selected from the group consisting of:
(i) peptides having the formula:
a-X1-b
wherein:
X1 is a sequence of at least six amino acids taken as a block from AA.sub.283 -AA.sub.309 of the gp46 env protein (HTLV-I), and analogues thereof;
a is an amino terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide; and
b is a carboxy terminus, one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide;
(ii) peptides having the formula:
a-X2-b
wherein:
X2 is a sequence of at least six amino acids taken as a block from AA.sub.273 -AA.sub.305 of the gp46 env protein (HTLV-II), and analogues thereof and a and b are as defined above;
(iii) peptides having the formula:
a-Z1-b
wherein:
Z1 is a sequence of at least six amino acids taken as a block from AA.sub.236 -AA.sub.257 of the gag p24 protein (HTLV-I), and analogues thereof and a and b are as defined above;
(iv) peptides having the formula:
a-Z2-b
wherein:
Z2 is a sequence of at least six amino acids taken as a block from AA.sub.242 -AA.sub.263 of the gag p24 protein (HTLV-II), and analogues thereof and a and b are as defined above;
(v) peptides having the formula:
a-B2-b
wherein:
B2 is a sequence of at least six amino acids taken as a block from AA.sub.171 -AA.sub.207 of the gp46 env protein (HTLV-II), and analogues thereof and a and b are as defined above; and
(vi) tandem peptides having the formula:
a-((((J).sub.n -c).sub.o -((U).sub.p).sub.q -d).sub.r).sub.s -b
wherein:
J and U are sequences of at least six amino acids taken as a block from sequences independently selected from the group consisting of AA.sub.193 -AA.sub.210 or AA.sub.283 -AA.sub.309 of the gp46 env protein (HTLV-I); AA.sub.236 -AA.sub.257 of the p24 gag protein (HTLV-I); AA.sub.120 -AA.sub.130 of the p19 gag protein (HTLV-I); AA.sub.171 -AA.sub.207 or AA.sub.273 -AA.sub.305 of the gp46 env protein (HTLV-II); AA.sub.242 -AA.sub.263 of the p24 gag protein (HTLV-II) and AA.sub.126 -AA.sub.136 of the p19 gag protein (HTLV-II); and analogues thereof;
a and b are as defined above;
c and d, if present, are independently selected from one to eight amino acids or a substituent effective to facilitate coupling or to improve the immunogenic or antigenic activity of the peptide;
n=1-5;
o=1-5 if c is present, otherwise o=1;
p=1-5;
q=1-5;
r=1-5 if d is present, otherwise r=1; and
s=1-5.
As used herein "analogues" denote amino acid insertions, deletions, substitutions and modifications at one or more sites in the peptide chain in that portion of it that consists of the block of the naturally occurring HTLV-I or HTLV-II amino acid sequences.
Preferred modifications and substitutions to the native amino acid sequence of the peptides of this invention are conservative ones (i.e., those having minimal influence on the secondary structure and hydropathic nature of the peptide). These include substitutions such as those described by Dayhoff in the Atlas of Protein Sequence and Structure 5, 1978 and by Argos in EMBO J. 8, 779-785, 1989. For example, amino acids belonging to one of the following groups represent conservative changes: ala, pro, gly, glu, asp, gln, asn, ser, thr; cys, ser, tyr, thr; val, ile, leu, met, ala, phe; lys, arg, his; and phe, tyr, trp, his. In like manner, methionine, an amino acid which is prone to oxidation may be replaced by norleucine. The preferred substitutions also include substitutions of D-isomers for the corresponding L-amino acids.
However, as described above, irrespective of such insertions, deletions, substitutions and modifications, the peptides of this invention must contain at least six amino acids taken as a block from one of the following: AA.sub.283 -AA.sub.309 of the gp46 env protein (HTLV-I); AA.sub.273 -AA.sub.305 of the gp46 product of the env gene (HTLV-II); AA.sub.236 -AA.sub.257 of the p24 product of the gag protein (HTLV-I); AA.sub.242 -AA.sub.263 of the p24 product of the gag protein (HTLV-II); and AA.sub.171 -AA.sub.207 of the gp46 product of the env protein (HTLV-II). Likewise, the tandem peptides of this invention must contain at least six amino acids taken as a block from J and U as defined above.
Preferably, in the peptide formulas of this invention, block sequences X1, X2, Z1, Z2, B2, J and U are sequences of at least 10 amino acids. More preferably, they are sequences of at least 15 amino acids. Even more preferably, they are sequences of at least 20 amino acids and most preferably, they are sequences of at least 25 amino acids. Preferably, in the tandem peptide formula of this invention, n, o, p, q, r and s=1-3. More preferably, n, o, p, q, r and s=1-2.
The term "amino acid" as employed in this description (e.g., in the definition of a and b) except when referring to the amino acid sequence of the gene products of the HTLV-I or HTLV-II, encompasses all of the natural amino acids, those amino acids in their D-configurations, and the known non-native, synthetic, and modified amino acids, such as homocysteine, ornithine, norleucine and .beta.-valine.
The term "block" as used herein refers to a sequence of amino acids identical to that present in a native polypeptide of HTLV-I or HTLV-II.
As reflected in (vi) above, it is within the scope of this invention to employ tandem peptides. These peptides may be homopolymers, copolymers or multimers. We have unexpectedly found that these tandem peptides show a very high degree of reactivity to HTLV antibodies. Physical mixtures of the peptides and tandem peptides of this invention are also within its scope.
Illustrative of peptides of this invention that are derived from gp46 of the env gene of HTLV-I is:
BCH-416 a-(SEQ ID NO:5)-b env(283-309)
wherein the cysteine residue in position 289 was replaced by a serine and also wherein a and b are as defined above.
Illustrative of peptides of this invention derived from gp46 of the env gene of HTLV-II are: ##STR1## wherein a and b are as defined above.
Illustrative of the peptides of this invention derived from p24 of the gag gene of HTLV-I is:
BCH-411 a-(SEQ ID NO:13)-b gag(236-257)
wherein a and b are as defined above.
Illustrative of the peptides derived from p24 of the gag gene of HTLV-II is:
BCH-412 a-(SEQ ID NO:14)-b gag(242-263)
wherein a and b are as defined above.
The preferred tandem peptides of this invention are defined by the formula:
a-J-c-U-b
wherein a, b, c, J and U are as defined above. Most preferably, one of J and U is selected from an HTLV-I peptide and the other is selected from an HTLV-II peptide. Illustrative of these preferred tandem peptides is:
BCH-234 a-(SEQ ID NO:15)-b
wherein:
J is BCH-152 (SEQ ID NO:16) (derived from the env gene of HTLV-I: env (193-210)) to which a Pro residue was added at the N-terminus (a) and a Leu residue at the C terminus was removed, c not being present and U being BCH-228 (SEQ ID NO:17) (derived from the gag gene of HTLV-I:, gag (120-130), the last 11 amino acids of p19 (FIG. 2)).
The preferred peptides of this invention are BCH-219, BCH-407, BCH-416, BCH-418, BCH-447, BCH-456, BCH-486 and tandem peptide BCH-234. The most preferred peptides of this invention are BCH-416, BCH-456, BCH-486 and BCH-234. The preferred peptide mixture of this invention contains at least BCH-416. More preferably, it contains at least BCH-416 and BCH-234 and most preferably, it contains at least BCH-416, BCH-234 and BCH-456.
In addition to their use in HTLV-I and II diagnostic means, methods and compositions, the peptides of this invention may also be used to distinguish and HTLV-I infection from an HTLV-II infection. Such diagnostic distinction is done, for example, by testing the sample separately with an HTLV-I derived peptide of this invention, or mixture thereof with other HTLV-I peptides and with an HTLV-II derived peptide of this invention or mixture thereof with other HTLV-II peptides and determining which peptide reacts most strongly. The preferred peptides for such methods are BCH-234 for the identification of an HTLV-I infection and BCH-456 and BCH-486 for the identification of an HTLV-II infection.
The peptides of this invention and mixtures thereof are also useful in vaccines against HTLV infections. Antibody titers against HTLV-I and II are notoriously low indicating that these viruses are not very immunogenic or bear immunosuppressive epitopes dampening the normal immune response. Use of synthetic peptides mimicking only those epitopes responsible for the induction of neutralizing antibodies may, thus, be of value in the development of candidate synthetic vaccines. Therefore, it is also within the scope of this invention to use these peptides in vaccines against HTLV-I and HTLV-II infections.
An unexpected advantage of the peptides and mixtures of this invention is their high sensitivity. They are capable of detecting HTLV-I/II-specific antibodies in the majority, and sometimes in all, of confirmed seropositive samples present in a commercial panel sold for the assessment of the performance of anti-HTLV-I/II kits. Another advantage of the peptides and mixtures of this invention is their high level of specificity--a minimal number of false positives. For example, using the above described peptide mixture (BCH-219, BCH-234 and BCH-416) no false-positive was recorded when 150 samples taken from the normal blood donor population were tested. Moreover, average ratio of O.D. recorded upon cut off was equal to 0.25 for these negative samples.
As set forth briefly above, it is often useful and certainly within the scope of this invention to modify the peptides of this invention in order to make the chosen peptide more useful as an immunodiagnostic reagent or as an active ingredient of a vaccine. Such changes, for example, include:
addition of a cysteine residue to one or both terminals in order to facilitate coupling of the peptide to a suitable carrier with heterobifunctional cross-linking reagents such as sulfosuccinimidyl-4-(p-maleimidophenyl) butyrate, preferred reagents for effecting such linkages are sulfosuccinimidyl- sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate and N-succinimidyl-3-(2-pyridyldithio) propionate;
addition of 1 to 8 additional amino acids at one or both terminals of the peptide to facilitate linking of the peptides to each other, for coupling to a support or larger peptide or protein or for modifying the physical or chemical properties of the peptide. Examples of such changes are the addition of N- or C-terminal tyrosine, glutamic acid or aspartic acid as linkers via an esterification reaction and lysine which can be linked via Schiff base or amide formation. As described above such additional amino acids may include any of the natural amino acids, those amino acids in their D-configurations, and the known non-native, synthetic and modified amino acids; and
derivatization of one or both terminals of the peptide by, for example, acylation or amidation. These modifications result in changes in the net charge on the peptide and can also facilitate covalent linking of the peptide to a solid support, a carrier or another peptide. Examples of the substituents effective to facilitate coupling or to improve the immunogenicity or antigenic activity of the peptide are C.sub.2 -C.sub.16 acyl groups, polyethylene glycol and phospholipids.
To prepare the novel peptides of this invention any of the conventional peptide production methodologies may be used. These include synthesis, recombinant DNA technology and combinations thereof. We prefer solid phase synthesis. In that synthetic approach, the resin support may be any suitable resin conventionally employed in the art for the solid phase preparation of peptides. Preferably, it is a p-benzyloxyalcohol polystyrene or p-methylbenzydrylamine resin. Following the coupling of the first protected amino acid to the resin support, the amino protecting group is removed by standard methods conventionally employed in the art. After removal of the amino protecting group, the remaining protected amino acids and, if necessary, side chain protected amino acids are coupled, sequentially, in the desired order to obtain the chosen peptide. Alternatively, multiple amino acid groups may be coupled using solution methodology prior to coupling with the resin-supported amino acid sequence.
The selection of an appropriate coupling reagent follows established art. For instance, suitable coupling reagents are N,N'-diisopropylcarbodiimide or N,N'-dicyclohexylcarbodiimide (DCC) or benzotriazol-1-yloxy-tris(dimethylamino)phosphonium hexafluoro-phosphate either alone or preferably in the presence of 1-hydroxybenzotriazole. Another useful coupling procedure employs preformed symmetrical anhydrides of protected amino acids.
The necessary .alpha.-amino protecting group employed for each amino acid introduced onto the growing polypeptide chain is preferably 9-fluorenylmethyloxycarbonyl (FMOC), although any other suitable protecting group may be employed as long as it does not degrade under the coupling conditions and is readily removable selectively in the presence of any other protecting group already present in the growing peptide chain.
The criteria for selecting protecting groups for the side chain amino acids are: (a) stability of the protecting group to the various reagents under reaction conditions selective for the removal of the .alpha.-amino protecting group at each step of the synthesis; (b) retention of the protecting group's strategic properties (i.e., not be split off under coupling conditions) and (c) removability of protecting group easily upon conclusion of the peptide synthesis and under conditions that do not otherwise affect the peptide structure.
The fully protected resin-supported peptides are preferably cleaved from the p-benzyloxy alcohol resin with 50% to 60% solution of trifluoroacetic acid in methylene chloride for 1 to 6 hours at room temperature in the presence of appropriate scavengers such as anisole, thioanisole, ethyl methyl sulfide, 1,2-ethanedithiol and related reagents. Simultaneously, most acid labile side-chain protecting groups are removed. More acid resistant protecting groups are typically removed by HF treatment.
The peptides of the present invention are useful as diagnostic reagents for the detection and quantification of HTLV-I and HTLV-II-associated antibodies in accordance with methods well-known in the art. These include ELISA, hemagglutination, single-dot and multi-dot methods and assays.
A preferred convenient and classical technique for the determination of antibodies against the HTLV-I and HTLV-II using a peptide or a peptide mixture of this invention is an enzyme-linked immunosorbent assay (ELISA). In this assay, for example, a peptide or mixture of this invention is adsorbed onto, or covalently coupled to, the wells of a microtiter plate. The wells are then treated with the sera or analyte to be tested. After washing, anti-human IgG or anti-human IgM labeled with peroxidase is added to the wells. The determination of the peroxidase is performed with a corresponding substrate, e.g., 3,3',5,5'-tetra-methylbenzidine. Without departing from the usefulness of this illustrative assay, the peroxidase can be exchanged by another label, e.g., by a radioactive, fluorescence, chemiluminescence or infra-red emitting label.
Another method for the determination of antibodies against the HTLV-I and HTLV-II with the peptides and mixtures of this invention is an enzyme immunological test according to the so-called "Double-Antigen-Sandwich-Assay". This method is based on the work of Maiolini, as described in Immunological Methods, 20, 25-34, 1978. According to this method, the serum or other analyte to be tested is contacted with a solid phase on which a peptide of this invention has been coated (capture layer) and with a peptide of this invention which has been labeled with peroxidase or other label (probe layer).
The immunological reaction can be performed in one or two steps. If the immunological reaction is performed in two steps, then a washing step is typically carried out between the two incubations. After the immunological reaction or reactions, a washing step is also usually performed. Thereafter, the peroxidase or other signal is determined, e.g., using o-phenylene diamine for peroxidase. Other enzymes and chromogens, including those already described, can also be employed in this assay.
Suitable solid phases for use in the above-described assays and assay methods include organic and inorganic polymers, e.g., amylases, dextrans, natural or modified celluloses, polyethylene, polystyrene, polyacrylamides, agaroses, magnetite, porous glass powder, polyvinyldiene fluoride (kynar) and latex, the inner wall of test vessels (i.e., test tubes, titer plates or cuvettes of glass or artificial material) as well as the surface of solid bodies (i.e., rods of glass and artificial material, rods with terminal thickening, rods with terminal lobes or lamellae). Spheres of glass and artificial material are especially suitable as solid phase carriers.
The peptides and mixtures of this invention are not only useful in the determination and quantification of antibodies against the HTLV-I and HTLV-II. They are also useful for the determination and quantification of HTLV-I and HTLV-II antigens themselves because the peptides of this invention, either free, polymerized or conjugated to an appropriate carrier, are useful in eliciting antibodies, in particular and preferably, monoclonal antibodies, immunologically cross reactive to the antigens of the HTLV-I and HTLV-II. Such antibodies, for example, can be produced by injecting a mammalian or avian animal with a sufficient amount of the peptide to elicit the desired immune response and recovering said antibodies from the serum of said animals. Suitable host animals for eliciting antibodies include, for example, rabbits, horses, goats, guinea pigs, rats, mice, cows, sheep and hens. Preferably, hybridomas producing the desired monoclonal antibodies are prepared using the peptides of this invention and conventional techniques.
For example, the well-known Kohler and Milstein technique for producing monoclonal antibodies may be used. In order to distinguish monoclonal antibodies which are directed against the same antigen, but against different epitopes, the method of Stahli et al. (J. of Immunological Methods, 32, 297-304, 1980) can be used.
Various methods which are generally known can be employed in the determination or quantification of the HTLV-I and HTLV-II or a portion thereof using the above antibodies. In one such procedure, known amounts of a serum sample or other analyte to be assayed, a radiolabeled peptide or mixture of this invention and an unlabeled peptide or mixture of this invention are mixed together, a given amount of an antipeptide antibody, preferably a monoclonal antibody, is added and the mixture allowed to stand. The resulting antibody/antigen complex is then separated from the unbound reagents by procedures known in the art, i.e., by treatment with ammonium sulphate, polyethylene glycol, a second antibody either in excess or bound to an insoluble support, or dextran-coated charcoal. The concentration of the labeled peptide is then determined in either the bound or unbound phase and the HTLV-I or HTLV-II antigen content of the sample determined by comparing the level of labeled component to a standard curve in a manner known per se.
Another suitable method for using these antibodies in assays is the "Double-Antibody-Sandwich-Assay". According to this assay, the sample to be tested is treated with two different antibodies, e.g., raised by immunizing different animals, e.g., sheep and rabbits, with a peptide or mixture of this invention. One of the antibodies is labeled and the other is coated on a solid phase. The preferred solid phase is a plastic bead and the preferred label is horse-radish peroxidase.
Typically in the "Double-Antibody-Sandwich-Assay", the sample is incubated with the solid phase antibody and the labeled antibody. However, it is also possible to contact the sample first with the solid phase antibody and then, after an optional washing, to contact the sample with the labeled antibody. Preferably, however, the sample is treated together with the solid phase and the labeled antibody. After the immunological reaction(s), the mixture is washed and the label is determined according to procedures known in the art. In the case where peroxidase is used as the label, the determination may be performed using a substrate, e.g., with o-phenylene diamine or with tetramethylbenzidine. The amount of the labeled component is proportional to the amount of the antigen(s) present in the analyte or serum sample.
The methods and assays for the determination and quantification of HTLV-I and HTLV-II antigens or antibodies against those viruses, as described above, can also be conducted in suitable test kits characterized by a peptide or mixture of this invention, or antibodies against HTLV-I or HTLV-II elicited by those peptides and mixtures.
As described above, the peptides and mixtures of this invention are also useful as the active component of vaccines capable of inducing protective immunity against the HTLV-I and HTLV-II in hosts susceptible to infection with those viruses. Routes of administration, antigen doses, number and frequency of injections will vary from individual to individual and parallel those currently being used in providing immunity to other viral infections. For example, the vaccines of this invention are pharmaceutically acceptable compositions containing at least one peptide or mixture of this invention in an amount effective in the mammal, including a human, treated with that composition to raise antibodies sufficient to protect the treated mammal from a HTLV-I or HTLV-II infection for a period of time.
The vaccines are prepared in accordance with known methods. The vaccine compositions of this invention may be conveniently and conventionally combined with physiologically acceptable carrier materials, such as pharmaceutical grade saline, tetanus toxoid, and keyhole limpet hemocyanin. The vaccine compositions of this invention may also contain adjuvants or other enhancers of immune response, such as alum preparations, liposomes or immunomodulators. Furthermore, these vaccine compositions may comprise other antigens to provide immunity against other viruses (e.g., HIV-1 and HIV-2, cytomegalovirus) or pathogens in addition to HTLV-I or HTLV-II. The amount of these other antigens is again dependent on the mammal to be treated and the course of the disease. However, the antigen should be present in an amount effective to raise antibodies sufficient to protect the treated mammal from that pathogen or virus for a period of time.
General procedures for the synthesis and utilization of the peptides of this invention are provided below.
PROCEDURE 1
Preparation of Resins Carrying the N-FMOC Protected Amino Acid Residue
The desired N-FMOC protected amino acid residue in a mixture of methylene chloride (CH.sub.2 Cl.sub.2) and dimethylformamide (DMF) (4:1) was added to a suspension of p-benzyloxy alcohol resin in CH.sub.2 Cl.sub.2: DMF (4:1) at 0.degree. C. The mixture was stirred manually for a few seconds and then treated with N,N'-dicyclohexyl-carbodiimide (DCC) followed by a catalytic amount of 4-(dimethylamino) pyridine. The mixture was stirred at 0.degree. C. for an additional 30 minutes and then at room temperature overnight. The filtered resin was washed successively with CH.sub.2 Cl.sub.2, DMF and isopropanol (3 washes each) and finally, with CH.sub.2 Cl.sub.2. The resin was suspended in CH.sub.2 Cl.sub.2, chilled in an ice bath and redistilled pyridine was added to the stirred suspension followed by benzoyl chloride. Stirring was continued at 0.degree. C. for 30 minutes and then at room temperature for 60 minutes. After filtration, the resin was washed successively with CH.sub.2 Cl.sub.2, DMF and isopropanol (3 washes each) and finally with petroleum ether (twice) before being dried under high vacuum to a constant weight.
Spectrophotometric determination of substitution according to Meienhofer et al. (Int. J. Peptide Protein Res., 13, 35, 1979) indicates the degree of substitution on the resin.
PROCEDURE 2
Coupling of Subsequent Amino Acids
The resin carrying the N-FMOC protected first amino acid residue was placed in a reaction vessel of a Biosearch 9600 Peptide Synthesizer and treated as follows:
1) Washed with DMF (4 times for 20 sec. each)
2) Prewashed with a 30% solution of piperidine in DMF (3 min.)
3) Deprotected with a 30% solution of piperidine in DMF (7 min.)
4) Washed with DMF (8 times for 20 sec. each)
5) Checked for free amino groups--Kaiser Test (must be positive)
6) The peptide resin was then gently shaken for 1 or 2 hrs with 8 equivalents of the desired FMOC-protected amino acid and 1-hydroxybenzotriazole and benzotriazol-1-yloxy-tris(dimethyl-amino)phosphonium hexafluorophosphate all dissolved in dry redistilled DMF containing 16 equivalents of 4-methylmorpholine.
7) Washed with DMF (6 times for 20 sec. each)
After step 7, an aliquot was taken for a ninhydrin test. If the test was negative, one goes back to step 1 for coupling of the next amino acid. If the test was positive or slightly positive, steps 6 and 7 should be repeated.
The above scheme may be used for coupling each of the amino acids of the peptides described in this invention. N-protection with FMOC may also be used with each of the remaining amino acids throughout the synthesis.
Radiolabeled peptides may be prepared by incorporation of a tritiated amino acid using the above coupling protocol.
After the addition of the last amino acid, the N-FMOC of the N-terminal residue is removed by going back to steps 1-7 of the above scheme. The peptide resin is washed with CH.sub.2 Cl.sub.2 and dried in vacuo to give the crude protected peptide.
PROCEDURE 3
Deprotection and Cleavage of the Peptides from the Resin
The protected peptide-resin was suspended in a 55% solution of trifluoroacetic acid (TFA) in CH.sub.2 Cl.sub.2, containing 2.5% ethanedithiol and 2.5% anisole. The mixture was flushed with N.sub.2 and stirred for 1.5 hours at room temperature. The mixture was filtered and the resin washed with CH.sub.2 Cl.sub.2. The resin was treated again with 20% TFA in CH.sub.2 Cl.sub.2 for 5 minutes at room temperature. The mixture was filtered and the resin washed with 20% TFA in CH.sub.2 Cl.sub.2 and then washed with CH.sub.2 Cl.sub.2. The combined filtrates were evaporated in vacuo below 35.degree. C. and the residue triturated several times with dry dimethyl ether. The solid was dissolved in 10% aqueous acetic acid and lyophilized to afford the crude product.
The peptides containing arg and cys residues are further deprotected by HF treatment at 0.degree. C. for 1 hour in the presence of anisole and dimethylsulfide. The peptides were extracted with 10% aqueous acetic acid, washed with dimethyl ether and lyophilized to afford the crude peptides.
PROCEDURE 4
Purification of Peptides
The crude peptides were purified by preparative HPLC on a Vydac column (2.5.times.25 mm) of C.sub.18 or C.sub.4 reverse phase with a gradient of the mobile phase. The effluent was monitored at 220 nm and subsequently by analytical HPLC. Relevant fractions were pooled, evaporated and lyophilized. The identity of the synthetic peptides was verified by analytical reverse phase chromatography and by amino acid analysis.
PROCEDURE 5
Conjugation of Peptides to Bovine Serum Albumin or Keyhole Limpet Hemocyanin
Peptides were conjugated to BSA or KLH previously derivatized with either sulfosuccinimidyl 4-(p-maleimidophenyl) butyrate (Sulfo-SMPB) or sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (Sulfo-SMCC).
An aqueous solution of sulfo-SMPB or sulfo-SMCC (Pierce Chemicals) was added to a solution of BSA or KLH in 0.02M sodium phosphate buffer (pH 7.0). The mixture was shaken at room temperature for 45 minutes and the activated carrier immediately applied to a Sephadex G-25 column equilibrated with 0.1M sodium phosphate buffer (pH 6.0) at 4.degree. C.
The fractions of the first peak absorbance (280 nm) corresponding to activated carrier were combined in a round bottom flask to which was added a solution of peptide in 0.05M sodium phosphate buffer (pH 6.2). The mixture was thoroughly flushed with N.sub.2 and incubated overnight at room temperature. The coupling efficiency was monitored using .sub.3 H-labeled peptide and by amino acid analysis of the conjugate.
PROCEDURE 6
Detection of Antibodies to HTLV-I or HTLV-II by an Enzyme Linked Immunosorbent Assay (ELISA)
Each well of the microtiter plate was saturated with 100 l of a solution containing a peptide or mixture of peptides (10 .mu.g/ml) and left overnight. The wells were emptied and washed twice with a washing buffer (Tris, 0.043M; NaCl, 0.5M; thimerosal, 0.01% w/v; Tween 20, 0.05% v/v; pH 7.4). The wells were then saturated with 0.35 ml of washing buffer for 1 hour at 37.degree. C. and washed once with the same buffer. Serum samples to be analyzed were diluted with specimen buffer (washing buffer plus casein, 0.05% w/v). The wells were rinsed with washing buffer prior to the addition of the diluted serum sample (0.1 ml). These were left to incubate for 1 hour at room temperature. The wells were then emptied, washed twice rapidly and then once for two minutes with washing buffer. The conjugate solution (peroxidase labeled affinity purified goat antibody to human IgG or to human IgM, 0.5 mg in 5 ml 50% glycerol) diluted with 1% w/v bovine serum albumin in washing buffer was added to each well (0.1 ml) and incubated for 1 hour at room temperature. The wells were then emptied and washed five times with the washing buffer. The substrate solution (3,3',5,5'-tetramethylbenzidine, 8 mg per ml of DMSO) was diluted with 100 volumes 0.1M citrate-acetate buffer (pH 5.6) containing 0.1% v/v of 30% H.sub.2 O.sub.2 and added to each well (0.1 ml per well). After 30 minutes, the contents of each well were treated with 0.1 ml 2N H.sub.2 SO.sub.4 and the optical density read at 450 nm. All determinations were done in duplicate.
Using general procedures substantially as described above, the following specific peptides were prepared: BCH-152, BCH-219, BCH-221, BCH-228, BCH-234, BCH-296, BCH-298, BCH-404, BCH-407, BCH-411, BCH-412, BCH-416, BCH-418, BCH-447, BCH-456 and BCH-486. These peptides were then evaluated for their ability to detect HTLV-I and HTLV-II-specific antibodies.
EXPERIMENT 1
In Experiment 1 individual peptides BCH-152, BCH-228, a cocktail formed by the mixture of equal amounts of BCH-152 and BCH-228, and individual peptide BCH-234 were compared in ELISA assays using a panel of seropositive and seronegative serum and plasma samples. BCH-234 is a tandem peptide wherein BCH-152 (with a Pro residue at the N-terminus) is attached to BCH-228. The results represent signal to cut off ratios and are displayed in Table 1.
TABLE 1______________________________________ EIA WITH SYNTHETIC PEPTIDESERUM 152 +NO. W.B. 152 228 228 234______________________________________BBI-168 NEG 0.50 0.58 0.50 0.44SC1*1/250 POS 8.71 5.94 -- 13.5B-12 POS 1.60 2.12 1.80 3.78B-14 POS 0.85 6.37 2.10 11.5B-18 POS 0.50 1.75 0.95 3.72B-20 POS 0.45 6.33 1.55 11.7B-21 POS 0.40 2.58 1.00 9.67B-22 POS 0.50 1.58 0.55 9.89B-23 POS 2.75 3.42 2.70 9.67B-27 POS 2.11 2.79 2.45 7.17B-30 POS 10.4 2.04 9.85 10.7B-31 POS 1.55 5.08 2.10 12.8A02-1 POS 0.39 0.45 0.30A02-2 POS >14 13.7 >14A02-3 POS 0.34 0.34 0.21A02-4 POS 0.75 0.76 0.40A02-5 POS 0.32 0.39 0.28A02-6 IND** 0.38 0.36 0.23A02-7 POS 0.89 0.79 2.72A02-8 POS 0.52 0.63 0.43A02-9 POS 0.78 0.66 0.47A02-10 POS 8.71 4.73 10.5______________________________________ **Indeterminable
In Table 1, the samples labeled B- were obtained from Japan and those labeled BBI-168, SC1* 1/250 and A02- are from the U.S. As mentioned above, infected American samples have antibody titers that are lower than those measured in blood samples from Japan.
In this experiment, the cut off above which a sample was considered positive for the presence of antibodies to HTLV-I and HTLV-II was defined as being equal to the O.D. value obtained with the negative control sample (BBI-168) plus 0.10. Results shown are signal to cut off ratios. Serum samples giving a ratio equal or above 1.0 are considered as positive.
Peptide BCH-152 recognized 8 of these 21 HTLV-I/HTLV-II seropositive samples. Peptide BCH-228 detected 14 out of 21 samples. Although peptide BCH-228 could detect all samples obtained from Japan (albeit with relatively low titers), it detected only two of the ten American samples. The origin of the sera thus lead to a net difference in sensitivity using these individual peptides.
Because peptides BCH-152 and BCH-228 represent distinct epitopes of the HTLV-I virus, we mixed them in an attempt to augment the sensitivity of detection. As indicated in Table 1, the signal measured with the mixture was, in all cases, lower than the signal measured with the best of the two peptides taken singly. There is, thus, no observed additive effect when these two peptides were mixed together.
Surprisingly, a tandem of these two peptides, e.g., peptide BCH-234, dramatically augmented the sensitivity of detection of antibodies to HTLV-I/II. In all cases, the signal/cut off ratio recorded was higher with tandem peptide BCH-234 than with the individual peptides or cocktail. BCH-234 detected 15 of the 21 samples tested. Furthermore, in addition to being able to detect an extra sample on the American panel (A02-7), the signal/cutoff ratios measured on the Japanese panel (B-series) with BCH-234 were, in all cases, higher than those obtained with the individual peptides.
EXPERIMENT 2
In Experiment 2, peptides BCH-219, BCH-221, BCH-296 and BCH-298 were tested in an ELISA using a panel of seropositive and seronegative serum and plasma samples obtained from Boston Biomedica Inc. (all U.S. strains).
The results represent signal to cut off ratios and are displayed in Table 2.
TABLE 2______________________________________SERUM EIA WITH SYNTHETIC PEPTIDENO. W.B. 219 221 298 296______________________________________BBI-163 NEG 0.20 0.33 0.50 0.21SC1*1/250 POS 0.30 0.39 12.74 9.50A02-1 POS 11.8 0.41 4.50 3.71A02-2 POS 0.55 0.71 >14 >14A02-3 POS 1.20 0.38 0.85 0.38A02-4 POS 0.55 0.61 2.30 0.62A02-5 POS 1.15 0.53 2.85 1.67A02-6 IND 0.40 0.35 0.60 0.42A02-7 POS 0.35 0.48 9.45 7.71A02-8 POS 4.15 0.52 1.65 1.00A02-9 POS 11.5 0.51 1.25 0.75A02-10 POS 0.55 0.55 12.6 8.92A02-11 IND 4.40 0.57 4.35 2.75A02-12 POS 1.15 0.44 2.85 1.83A02-13 IND 0.80 0.43 1.45 1.04A02-14 POS 9.75 0.44 12.1 8.71A02-15 POS 3.30 0.37 6.00 2.71A02-16 POS 1.60 0.41 3.00 2.04A02-17 IND 0.80 0.39 1.20 2.75A02-18 POS 3.20 3.68 11.1 1.46A02-19 POS 10.0 0.41 0.85 2.21A02-20 POS 0.45 0.34 >14 >14A02-21 POS 4.85 0.38 3.05 2.71A02-22 POS 4.60 0.36 4.00 2.25A02-23 IND 5.15 0.54 3.95 2.33A02-24 POS >14 0.35 13.6 3.12A02-25 POS 2.55 0.49 1.75 1.21______________________________________
Peptide BCH-219 detected 17 out of the 26 seropositive samples tested. More importantly, peptide BCH-219 could detect the presence of HTLV antibodies in samples that were not detectable with BCH-234 (see Table 1). Peptide BCH-298 (a-TTDNSNNSIILPPFSLAPVPPP ATRRRR-b; HTLV-II env 281-308), which is a longer version of peptide BCH-221 and which has a serine residue at position 285 instead of the natural cysteine residue (this was done to avoid dimerization of the peptide, to increase its stability and to increase the specificity of the test), detected 23 out of 26 samples. This latter result illustrates the importance of residues env(281-292) which are absent on the poorly performing peptide BCH-221 (a-PFSLAPVPPPATRRRR-b HTLV-II env(293-308)) and which contribute largely to the increased antigenicity of peptide BCH-298. Peptide BCH-296 (a-AIVSSPSHNSLILPPFSLSPVPTLGSRSRR-b HTLV-I env (283-312)) detected 22 out of 26 samples but the signals were in general lower than those obtained when using BCH-298. It was later found, however, that when peptides BCH-298 or BCH-296 were used in a cocktail, they were responsible for the detection of a large number of false positives. For example, the screening of 500 samples obtained from normal blood donors lead to 38 putative seropositives when BCH-298 was part of a three-peptide cocktail. Different peptide analogs were synthesized in order to identify and eliminate the regions on BCH-298 and on BCH-296 which were responsible for these non-specific responses. Peptides BCH-404, BCH-407, BCH-416 and BCH-418 were particularly useful to achieving this goal (see Table 3).
EXPERIMENT 3
In Experiment 3, solutions prepared with each of the following peptides: BCH-404, BCH-407, BCH-416 and BCH-418, at 10 .mu.g/ml, were used to coat EIA plates as described above. A cocktail of peptides was also prepared by the mixing of equal volumes of three of the peptide solutions described above (BCH-219, BCH-234 and BCH-416) and used to coat another series of plates. All seropositive samples tested were obtained from Boston Biomedica Inc.
The results represent signal to cut off ratios and are displayed in Table 3. In this experiment the cut off (0.20) is the average O.D. measured on 50 samples taken from the normal blood donor population to which were added 4 standard deviations. (Cut off=X.sub.NEG +4 S.D.)
TABLE 3______________________________________EIA WITH SYNTHETIC PEPTIDESERUM 219 +No. 404 407 416 418 234 + 416______________________________________A02-01 0.74 0.81 7.17 9.21 >23.33A02-02 >10.37 >23.33 >23.33 >23.33 >23.33A02-03 0.63 0.31 0.38 0.38 1.20A02-04 0.87 1.13 6.25 0.99 6.31A02-05 0.81 1.34 2.42 5.26 7.46A02-06 0.66 0.23 0.24 0.16 0.66A02-07 7.20 18.78 18.33 22.92 >23.33A02-08 0.67 0.63 2.53 2.45 7.42A02-09 0.96 1.58 7.93 1.72 >23.33A02-10 1.15 7.17 22.33 >23.33 >23.33A02-11 0.68 1.39 7.80 7.78 16.77A02-12 0.77 1.53 2.74 6.00 7.87A02-13 0.63 0.49 2.02 2.37 5.77A02-14 5.78 15.67 22.28 >23.33 >23.33A02-15 2.33 11.32 4.76 9.52 16.43A02-16 0.71 0.43 5.38 6.17 13.39A02-17 0.67 0.46 1.51 1.79 2.17A02-18 0.69 0.52 2.95 >23.33 8.06A02-19 0.65 0.37 0.80 0.66 7.17A02-20 1.10 10.03 >23.33 >23.33 >23.33A02-21 0.90 1.59 3.73 4.95 9.14A02-22 0.91 1.48 5.86 7.13 22.23A02-23 1.10 1.98 5.93 6.97 18.39A02-24 4.13 22.01 13.82 >23.33 > 23.33A02-25 0.83 0.98 2.17 2.56 5.87______________________________________
Peptides BCH-404, BCH-407 and BCH-418 were synthesized in order to find one that would keep the same levels of sensitivity as BCH-298 (a-TTDNSNNSIILPPFSLAPVPPPATRRRR-b HTLV-II env (281-308)) but would not lead to false-positive signals. Peptide BCH-418 is as useful as BCH-298 in detecting the seropositive samples and did not give false-positive signals when tested on 500 serum samples taken from normal blood donors. Thus BCH-418 (which is identical to BCH-298 with the exception that the last three arginine residues were absent) is as sensitive as BCH- 298 in detecting HTLV-I and HTLV-II antibodies and also much more specific. A similar observation was made with synthetic peptide BCH-416, which is a shorter version of HTLV-I peptide BCH-296. As a result, we have concluded that these three amino acid residues located near the C-terminal end of the gp46 protein of both HTLV-I and HTLV-II contribute to the binding of antibody molecules not related to HTLV-I and HTLV-II infection.
We also tested a peptide cocktail including a preferred HTLV-I tandem peptide BCH-234 and peptides derived from the sequence of HTLV-II (BCH-219 and BCH-416). The results presented in Table 3 show that this cocktail can surprisingly detect all confirmed HTLV-I and HTLV-II seropositive samples tested. Specimen A02-6, which was found indeterminate by Western Blot and by immunofluorescence (IFA) was found negative in this test. That sample was found weakly positive in one test out of two.
EXPERIMENT 4
In Experiment 4, solutions prepared with synthetic peptides BCH-411 and BCH-412 at 10 .mu.g/ml, were used to coat EIA plates as described above. Samples tested were obtained from Japan.
The results represent signal to cut off ratios and are displayed in Table 4. The cut off in this experiment is the average of O.D. values obtained with three negative samples to which 4 S.D. were added.
TABLE 4______________________________________ EIA WITHSERUM SYNTHETIC PEPTIDENo. 411 412______________________________________B-12 1.74 1.34B-13 3.55 1.21B-14 9.84 2.42B-15 6.50 1.97B-16 2.64 2.24B-17 14.43 2.26B-18 1.81 1.39B-19 2.01 1.95B-20 7.17 1.47B-21 2.70 1.87B-22 1.78 1.89B-23 3.51 1.05B-24 4.61 1.55B-25 2.74 2.16B-26 12.09 2.24B-27 2.38 2.32B-28 10.56 1.92B-29 1.90 2.26B-30 5.39 2.16B-31 34.54 3.55______________________________________
EXPERIMENT 5
In Experiment 5, peptides BCH-219, BCH-447, BCH-456 and BCH-486 were tested in ELISA using a panel of seropositive and seronegative serum and plasma samples obtained from Boston Biomedica Inc.
The results (Table 5) represent the signal to cut off ratios. The cut off is the average of the absorbancy values measured with three negative serum samples to which was added 0.100.
Peptide BCH-219 detected 18 out of the 25 seropositive samples tested (Table 5). Two of the samples (No. A02-6 and A02-17) missed by BCH-219 are WB indeterminate. In this experiment, sample A02-2, which was taken from an HTLV-I infected patient was found positive. This was not always the case. In some other experiments, this sample was not detected as positive with BCH-219. Similarly, sample A02-16, which is not detected positive in this experiment, could in other tests be detected by BCH-219.
Peptide BCH-447 detected all HTLV-I and HTLV-II samples tested. Peptide BCH-456 did not detect any of the four HTLV-I samples and was also negative with one WB-indeterminate sample (A02-6). The profile or reactivity of BCH-486 is similar to BCH-456. Although the signals measured with BCH-486 are generally higher than those obtained with BCH-456, the latter is preferred because BCH-486 gave a borderline negative signal (0.94) with one HTLV-II sample (A02-4).
TABLE 5______________________________________SERUM EIA WITH SYNTHETIC PEPTIDENO. W.B. 219 447 456 486______________________________________A02-1 HTLV-II 14.99 18.75 9.09 16.67A02-2 HTLV-I 1.91 1.81 0.60 0.50A02-3 HTLV-II 1.62 4.38 1.91 3.50A02-4 HTLV-II 0.73 1.50 1.30 0.94A02-5 HTLV-II 1.19 8.25 8.45 5.00A02-6 Ind. 0.51 1.19 0.15 0.33A02-7 HTLV-I 0.57 1.31 0.30 0.50A02-8 HTLV-II 3.91 10.75 5.09 11.17A02-9 HTLV-II 17.65 16.75 8.12 15.39A02-10 HTLV-I 0.52 1.44 0.39 0.56A02-11 HTLV-II 4.42 9.63 4.91 9.28A02-12 HTLV-II 1.72 9.06 7.88 4.72A02-13 Ind. 1.11 2.56 5.55 1.72A02-14 HTLV-II 10.74 15.25 8.30 16.67A02-15 HTLV-II 3.65 6.50 3.52 6.61A02-16 HTLV-II 0.58 12.50 6.12 12.17A02-17 Ind. 0.86 2.06 1.36 1.50A02-18 HTLV-II 2.95 6.81 3.15 5.61A02-19 HTLV-II 12.83 14.38 9.09 16.67A02-20 HTLV-I 0.64 1.25 0.12 0.22A02-21 HTLV-II 3.68 11.31 6.18 10.50A02-22 HTLV-II 6.45 9.69 5.33 10.78A02-23 HTLV-II 6.20 10.44 5.48 11.33A02-24 HTLV-II 17.65 18.75 9.09 16.67A02-25 HTLV-II 2.62 6.31 4.94 6.39______________________________________
EXPERIMENT 6
In Experiment 6, three cocktails of peptides were prepared (10 .mu.g total peptide/ml of solution) and used to coat EIA plates as described above. The composition of each peptide cocktail and the results obtained are described in Table 6. All samples tested were bought from Boston Biomedica Inc. and the results are expressed as signal to cut off ratios as explained before.
The first cocktail containing BCH-219 did not detect samples A02-4 and A02-6; sample A02-3 was borderline negative. Although sample A02-6 is WB-indeterminate, samples A02-3 and A02-4 are confirmed HTLV-II positive and should not be missed. The third cocktail, containing BCH-447, also missed samples 4 and 6 but could recognize sample A02-3 as positive. The signals recorded with the BCH-447 cocktail were higher than those measured using the BCH-219 cocktail. Finally, the cocktail containing BCH-456 detected all samples confirmed to be HTLV-I or HTLV-II positive and, in most cases, ratios were higher than with the other two cocktails. This experiment showed that a cocktail composed of BCH-234, BCH-456 and BCH-416 gives the best sensitivity in detecting both HTLV-I and HTLV-II seropositive samples.
In this experiment, 36 samples (No. 301 to 336) taken from a normal blood donor population were also tested. All were found negative but sample 310 which gave a strong positive signal when measured on the peptide cocktail containing BCH-219. This sample was later confirmed negative by WB. Finally, another sample, E8-2109-272, was similarly found positive with the BCH-234:BCH-219:BCH-416 cocktail although it was WB negative. This sample did not give a false signal on the other two peptide cocktails thus demonstrating their higher degree of specificity.
Experiment 6 demonstrated the superiority of peptide BCH-447, and more preferably BCH-456, over BCH-219 in increasing both the sensitivity and specificity of a peptide cocktail aimed at detecting HTLV-I and HTLV-II specific antibodies.
TABLE 6______________________________________SERUM EIA WITH SYNTHETIC PEPTIDENO. 234:219:416 234:447:416 234:456:416______________________________________A02-1 6.99 13.95 12.94A02-2 13.95 13.95 15.00A02-3 0.96 1.35 1.33A02-4 0.68 0.87 1.44A02-5 1.40 2.24 8.45A02-6 0.30 0.54 0.60A02-7 6.92 11.07 12.61A02-8 2.75 5.71 6.06A02-9 13.95 13.95 12.02A02-10 13.95 13.95 15.00A02-11 3.73 5.51 6.65A02-12 1.79 2.88 9.15A02-13 1.17 2.08 2.78A02-14 10.71 13.95 15.00A02-15 3.87 5.75 7.09A02-16 2.80 6.33 5.77A02-17 1.13 1.69 1.54A02-18 2.03 2.65 3.70A02-19 5.46 13.95 13.46A02-20 13.95 13.95 15.00A02-21 2.23 3.70 5.72A02-22 5.56 7.49 8.75A02-23 4.61 7.90 9.20A02-24 13.95 13.95 15.00A02-25 1.91 3.19 3.32301 0.29 0.39 0.51302 0.49 0.56 0.36303 0.49 0.48 0.50304 0.39 0.41 0.39305 0.51 0.38 0.52306 0.33 0.53 0.53307 0.38 0.56 0.50308 0.33 0.7 0.44309 0.37 0.62 0.50310 4.29 0.73 0.48311 0.42 0.46 0.57312 0.48 0.42 0.43313 0.33 0.48 0.48314 0.56 0.59 0.36315 0.33 0.76 0.52316 0.58 0.60 0.55317 0.28 0.54 0.40318 0.47 0.61 0.36319 0.62 0.56 0.52320 0.49 0.51 0.42321 0.41 0.57 0.54322 0.33 0.52 0.53323 0.32 0.71 0.30324 0.59 0.53 0.56325 0.46 0.56 0.34326 0.30 0.44 0.51327 0.36 0.43 0.52328 0.29 0.40 0.52329 0.33 0.51 0.54330 0.39 0.55 0.54331 0.41 0.56 0.24332 0.50 0.60 0.31333 0.57 0.67 0.40334 0.40 0.53 0.43335 0.59 0.47 0.44336 0.52 0.59 0.33E8-2109-272 2.68 0.77 0.51______________________________________
EXPERIMENT 7
In Experiment 7, 25 serum samples obtained from patients identified by polymerase chain reaction (PCR) as being HTLV-I (HT-100 series) or HTLV-II (HT-200 series) carriers were analyzed on plates coated with BCH-234 (HTLV-I specific) or with BCH-219 or BCH-456 (HTLV-II specific). Results displayed in Table 7 are signal to cut off ratios calculated as stated above.
Table 7 illustrates the ease with which peptides BCH-234 and BCH-456 can selectively distinguish samples obtained from a patient infected with HTLV-I from one infected with HTLV-II. Samples from the five HTLV-I infected patients give a strong signal when tested with the HTLV-I specific peptide (BCH-234) and only a low signal on BCH-219. It is noteworthy that these five samples are all negative when tested on the HTLV-II specific peptide BCH-456. Furthermore, the 20 samples taken from HTLV-II infected patients are all easily detected with BCH-456 thus showing its excellent capacity in selectively discriminating samples from HTLV-II infected patients from those taken from an HTLV-I infected one.
TABLE 7______________________________________ EIA WITH SYNTHETIC PEPTIDESERUM PCR BCH-234 BCH-219 BCH-456NO. ANALYSIS (HTLV-I) (HTLV-II) (HTLV-II)______________________________________HT-101 HTLV-I 11.11 0.62 0.85HT-102 HTLV-I 11.11 1.07 0.97HT-103 HTLV-I 11.11 1.92 0.79HT-104 HTLV-I 11.11 1.12 0.61HT-105 HTLV-I 8.72 1.32 0.75HT-201 HTLV-II 1.03 5.52 25.00HT-202 HTLV-II 1.68 1.86 16.63HT-203 HTLV-II 0.81 2.25 10.44HT-204 HTLV-II 0.75 3.34 15.07HT-205 HTLV-II 1.07 9.09 25.00HT-206 HTLV-II 1.43 9.09 25.00HT-207 HTLV-II 1.82 1.94 14.37HT-208 HTLV-II 1.75 5.82 21.03HT-209 HTLV-II 0.91 4.05 25.00HT-210 HTLV-II 0.90 1.58 6.38HT-211 HTLV-II 0.76 9.09 25.00HT-212 HTLV-II 0.67 6.87 22.54HT-213 HTLV-II 1.17 2.42 8.53HT-214 HTLV-II 1.48 6.87 25.00HT-215 HTLV-II 1.85 9.09 25.00HT-216 HTLV-II 1.09 2.98 17.37HT-217 HTLV-II 1.15 1.43 9.57HT-218 HTLV-II 1.16 0.67 1.75HT-219 HTLV-II 0.66 0.61 3.78HT-220 HTLV-II 0.99 5.79 16.75______________________________________
While we have herein before described a number of embodiments of this invention, it is apparent that our basic constructions can be altered to provide other embodiments which utilize the processes and compositions of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the claims appended hereto rather than by the specific embodiments which have been presented herein before by way of example.
__________________________________________________________________________SEQUENCE LISTING(1) GENERAL INFORMATION:(iii) NUMBER OF SEQUENCES: 20(2) INFORMATION FOR SEQ ID NO:1:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 488 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:MetGlyLysPheLeu AlaThrLeuIleLeuPhePheGlnPheCysPro151015LeuIlePheGlyAspTyrSerProSerCysCysThrLeuThrIleGly20 2530ValSerSerTyrHisSerLysProCysAsnProAlaGlnProValCys354045SerTrpThrLeuAspLeu LeuAlaLeuSerAlaAspGlnAlaLeuGln505560ProProCysProAsnLeuValSerTyrSerSerTyrHisAlaThrTyr6570 7580SerLeuTyrLeuPheProHisTrpThrLysLysProAsnArgAsnGly859095GlyGlyTyrTyrSerAla SerTyrSerAspProCysSerLeuLysCys100105110ProTyrLeuGlyCysGlnSerTrpThrCysProTyrThrGlyAlaVal115 120125SerSerProTyrTrpLysPheGlnHisAspValAsnPheThrGlnGlu130135140ValSerArgLeuAsnIleAsnLeuHisP heSerLysCysGlyPhePro145150155160PheSerLeuLeuValAspAlaProGlyTyrAspProIleTrpPheLeu165 170175AsnThrGluProSerGlnLeuProProThrAlaProProLeuLeuPro180185190HisSerAsnLeuAspHisIl eLeuGluProSerIleProTrpLysSer195200205LysLeuLeuThrLeuValGlnLeuThrLeuGlnSerThrAsnTyrThr210215 220CysIleValCysIleAspArgAlaSerLeuSerThrTrpHisValLeu225230235240TyrSerProAsnValSerValPro SerSerSerSerThrProLeuLeu245250255TyrProSerLeuAlaLeuProAlaProHisLeuThrLeuProPheAsn260 265270TrpThrHisCysPheAspProGlnIleGlnAlaIleValSerSerPro275280285CysHisAsnSerLeuIleLeuPro ProPheSerLeuSerProValPro290295300ThrLeuGlySerArgSerArgArgAlaValProValAlaValTrpLeu305310 315320ValSerAlaLeuAlaMetGlyAlaGlyValAlaGlyGlyIleThrGly325330335SerMetSerLeuAlaSerGlyL ysSerLeuLeuHisGluValAspLys340345350AspIleSerGlnLeuThrGlnAlaIleValLysAsnHisLysAsnLeu355 360365LeuLysIleAlaGlnTyrAlaAlaGlnAsnArgArgGlyLeuAspLeu370375380LeuPheTrpGluGlnGlyGlyLeuCysLysAl aLeuGlnGluGlnCys385390395400ArgPheProAsnIleThrAsnSerHisValProIleLeuGlnGluArg405 410415ProProLeuGluAsnArgValLeuThrGlyTrpGlyLeuAsnTrpAsp420425430LeuGlyLeuSerGlnTrpAlaArg GluAlaLeuGlnThrGlyIleThr435440445LeuValAlaLeuLeuLeuLeuValIleLeuAlaGlyProCysIleLeu450455 460ArgGlnLeuArgHisLeuProSerArgValArgTyrProHisTyrSer465470475480LeuIleLysProGluSerSerLeu 485(2) INFORMATION FOR SEQ ID NO:2:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 486 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:2:MetGlyAsnValPhePheLeuLeuLeuPheSerLeuThrHisPhePr o151015LeuAlaGlnGlnSerArgCysThrLeuThrIleGlyIleSerSerTyr202530 HisSerSerProCysSerProThrGlnProValCysThrTrpAsnLeu354045AspLeuAsnSerLeuThrThrAspGlnArgLeuHisProProCysPro 505560AsnLeuIleThrTyrSerGlyPheHisLysThrTyrSerLeuTyrLeu65707580Ph eProHisTrpIleLysLysProAsnArgGlnGlyLeuGlyTyrTyr859095SerProSerTyrAsnAspProCysSerLeuGlnCysProTyrLeuGly 100105110CysGlnAlaTrpThrSerAlaTyrThrGlyProValSerSerProSer115120125Trp LysPheHisSerAspValAsnPheThrGlnGluValSerGlnVal130135140SerLeuArgLeuHisPheSerLysCysGlySerSerMetThrLeuLeu145 150155160ValAspAlaProGlyTyrAspProLeuTrpPheIleThrSerGluPro165170175T hrGlnProProProThrSerProProLeuValHisAspSerAspLeu180185190GluHisValLeuThrProSerThrSerTrpThrThrLysIleLeuLys 195200205PheIleGlnLeuThrLeuGlnSerThrAsnTyrSerCysMetValCys210215220ValAspArgSe rSerLeuSerSerTrpHisValLeuTyrThrProAsn225230235240IleSerIleProGlnGlnThrSerSerArgThrIleLeuPheProSer 245250255LeuAlaLeuProAlaProProSerGlnProPheProTrpThrHisCys260265270Tyr GlnProArgLeuGlnAlaIleThrThrAspAsnCysAsnAsnSer275280285IleIleLeuProProPheSerLeuAlaProValProProProAlaThr 290295300ArgArgArgArgAlaValProIleAlaValTrpLeuValSerAlaLeu305310315320AlaAla GlyThrGlyIleAlaGlyGlyValThrGlySerLeuSerLeu325330335AlaSerSerLysSerLeuLeuLeuGluValAspLysAspIleSerHis 340345350LeuThrGlnAlaIleValLysAsnHisGlnAsnIleLeuArgValAla355360365GlnTyrA laAlaGlnAsnArgArgGlyLeuAspLeuLeuPheTrpGlu370375380GlnGlyGlyLeuCysLysAlaIleGlnGluGlnCysCysPheLeuAsn385 390395400IleSerAsnThrHisValSerValLeuGlnGluArgProProLeuGlu405410415LysAr gValIleThrGlyTrpGlyLeuAsnTrpAspLeuGlyLeuSer420425430GlnTrpAlaArgGluAlaLeuGlnThrGlyIleThrIleLeuAlaLeu 435440445LeuLeuLeuValIleLeuPheGlyProCysIleLeuArgGlnIleGln450455460AlaLeuProGlnArg LeuGlnAsnArgHisAsnGlnTyrSerLeuIle465470475480AsnProGluThrMetLeu485(2) INFORMATION FOR SEQ ID NO:3:(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 429 amino acids(B) TYPE: amino acid(C) STRANDEDNESS: single(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:3:MetGlyGlnIlePheSerArgSerAlaSerProIleProArgProPro15 1015ArgGlyLeuAlaAlaHisHisTrpLeuAsnPheLeuGlnAlaAlaTyr202530ArgLeuGluProGlyProSer SerTyrAspPheHisGlnLeuLysLys354045PheLeuLysIleAlaLeuGluThrProAlaArgIleCysProIleAsn5055 60TyrSerLeuLeuAlaSerLeuLeuProLysGlyTyrProGlyArgVal65707580AsnGluIleLeuHisIleLeuIleGln ThrGlnAlaGlnIleProSer859095ArgProAlaProProProProSerSerProThrHisAspProProAsp100 105110SerAspProGlnIleProProProTyrValGluProThrAlaProGln115120125ValLeuProValMetHisProHisGlyA laProProAsnHisArgPro130135140TrpGlnMetLysAspLeuGlnAlaIleLysGlnGluValSerGlnAla14515015 5160AlaProGlySerProGlnPheMetGlnThrIleArgLeuAlaValGln165170175GlnPheAspProThrAlaLysAspLe uGlnAspLeuLeuGlnTyrLeu180185190CysSerSerLeuValAlaSerLeuHisHisGlnGlnLeuAspSerLeu195200 205IleSerGluAlaGluThrArgGlyIleThrGlyTyrAsnProLeuAla210215220GlyProLeuArgValGlnAlaAsnAsnProGlnGln GlnGlyLeuArg225230235240ArgGluTyrGlnGlnLeuTrpLeuAlaAlaPheAlaAlaLeuProGly245 250255SerAlaLysAspProSerTrpAlaSerIleLeuGlnGlyLeuGluGlu260265270ProTyrHisAlaPheValGluArgLeu AsnIleAlaLeuAspAsnGly275280285LeuProGluGlyThrProLysAspProIleLeuArgSerLeuAlaTyr290295 300SerAsnAlaAsnLysGluCysGlyLysLeuLeuGlnAlaArgGlyHis305310315320ThrAsnSerProLeuGlyAspMetLeuArgA laCysGlnThrTrpThr325330335ProLysAspLysThrLysValLeuValValGlnProLysLysProPro34034 5350ProAsnGlnProCysPheArgCysGlyLysAlaGlyHisTrpSerArg355360365AspCysThrGlnProArgProProProGlyPr oCysProLeuCysGln370375380AspProThrHisTrpLysArgAspCysProArgLeuLysProThrIle385390395 400ProGluProGluProGluGluAspAlaLeuLeuLeuAspLeuProAla405410415AspIleProHisProLysAsnSerIleGly GlyGluVal420425(2) INFORMATION FOR SEQ ID NO:4:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 433 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: protein(xi) SEQUENCE DESCRIPTION: SEQ ID NO:4:MetGlyGlnIleHisGlyLeuS erProThrProIleProLysAlaPro151015ArgGlyLeuSerThrHisHisTrpLeuAsnPheLeuGlnAlaAlaTyr20 2530ArgLeuGlnProArgProSerAspPheAspPheGlnGlnLeuArgArg354045PheLeuLysLeuAlaLeuLysThrP roIleTrpLeuAsnProIleAsp505560TyrSerLeuLeuAlaSerLeuIleProLysGlyTyrProGlyArgVal6570 7580ValGluIleIleAsnIleLeuValLysAsnGlnValSerProSerAla859095ProAlaAlaProValProThrProI leCysProThrThrThrProPro100105110ProProProProProSerProGluAlaHisValProProProTyrVal11512 0125GluProThrThrThrGlnCysPheProIleLeuHisProProGlyAla130135140ProSerAlaHisArgProTrpGlnMetLysAspLe uGlnAlaIleLys145150155160GlnGluValSerSerSerAlaLeuGlySerProGlnPheMetGlnThr165 170175LeuArgLeuAlaValGlnGlnPheAspProThrAlaLysAspLeuGln180185190AspLeuLeuGlnTyrLeuCysSerSer LeuValValSerLeuHisHis195200205GlnGlnLeuAsnThrLeuIleThrGluAlaGluThrArgGlyMetThr210215 220GlyTyrAsnProMetAlaGlyProLeuArgMetGlnAlaAsnAsnPro225230235240AlaGlnGlnGlyLeuArgArgGluTyrGln AsnLeuTrpLeuAlaAla245250255PheSerThrLeuProGlyAsnThrArgAspProSerTrpAlaAlaIle2602 65270LeuGlnGlyLeuGluGluProTyrCysAlaPheValGluArgLeuAsn275280285ValAlaLeuAspAsnGlyLeuProGluGlyT hrProLysGluProIle290295300LeuArgSerLeuAlaTyrSerAsnAlaAsnLysGluCysGlnLysIle305310315 320LeuGlnAlaArgGlyHisThrAsnSerProLeuGlyGluMetLeuArg325330335ThrCysGlnAlaTrpThrProLysAspLy sThrLysValLeuValVal340345350GlnProArgArgProProProThrGlnProCysPheArgCysGlyLys355360 365ValGlyHisTrpSerArgAspCysThrGlnProArgProProProGly370375380ProCysProLeuCysGlnAspProSerHisTrpLysArg AspCysPro385390395400GlnLeuLysProProGlnGluGluGlyGluProLeuLeuLeuAspLeu405410 415ProSerThrSerGlyThrThrGluGluLysAsnSerLeuArgGlyGlu420425430Ile(2) INFORMATION FOR SEQ ID NO:5:(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5:AlaIleValSerSerProSerHisAsnSerLeuIleLeuProProPhe1510 15SerLeuSerProValProThrLeuGlySerArg2025(2) INFORMATION FOR SEQ ID NO:6:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:6:LeuValHisAspSerAspLeuGluHisValLeuThrProSerThrSer151015TrpThrThrLysIleLeu20 (2) INFORMATION FOR SEQ ID NO:7:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 19 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:7:ThrThrAspAsnSerAsnAsnSerIleIleLeuProProPheSerLeu1 51015AlaProVal(2) INFORMATION FOR SEQ ID NO:8:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 27 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:8:TyrGlnProArgLeuGl nAlaIleThrThrAspAsnSerAsnAsnSer151015IleIleLeuProProPheSerLeuAlaProVal2025(2) INFORMATION FOR SEQ ID NO:9:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 25 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:9:ThrThrAspAsnSerAsnAsnSerIleIleLeuProProPheSerLeu1 51015AlaProValProProProAlaThrArg2025(2) INFORMATION FOR SEQ ID NO:10:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 31 amino acids(B) TYPE: amino acid (D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:10:PheIleThrSerGluProThrGlnProProProThrSerProProLeu151015ValHisAspSerAsp LeuGluHisValLeuThrProSerThrSer202530(2) INFORMATION FOR SEQ ID NO:11:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 24 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO:11:ThrSerGluProThrGlnProProProThrSerProProLeuValHis151015AspSerAspLeuGluHisValLeu 20(2) INFORMATION FOR SEQ ID NO:12:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 25 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:12:ThrSerGluProThrGlnProProProThrSerProProLeuLeuVal1 51015HisAspSerAspLeuGluHisValLeu2025(2) INFORMATION FOR SEQ ID NO:13:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 amino acids(B) TYPE: amino acid (D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:13:GlnGlnGlyLeuArgArgGluTyrGlnGlnLeuTrpLeuAlaAlaPhe151015AlaAlaLeuP roGlySer20(2) INFORMATION FOR SEQ ID NO:14:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 22 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:14:GlnGlnGlyLeuArgArgGluTyrGlnAsnLeuTrpLeu AlaAlaPhe151015SerThrLeuProGlyAsn20(2) INFORMATION FOR SEQ ID NO:15:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 29 amino acids(B) TYPE: amino acid (D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:15:ProHisSerAsnLeuAspHisIleLeuGluProSerIleProTrpLys151015SerLysProTyrVal GluProThrAlaProGlnValLeu2025(2) INFORMATION FOR SEQ ID NO:16:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 18 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:16:HisSerAs nLeuAspHisIleLeuGluProSerIleProTrpLysSer151015LysLeu(2) INFORMATION FOR SEQ ID NO:17:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 11 amino acids(B) TYPE: amino acid (D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:17:ProTyrValGluProThrAlaProGlnValLeu1510(2) INFORMATION FOR SEQ ID NO:18:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 33 amino acids(B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:18:TyrGlnProArgLeuGlnAlaIleThrThrAspAsnCysAsnAsnSer151015IleIleL euProProPheSerLeuAlaProValProProProAlaThr202530Arg(2) INFORMATION FOR SEQ ID NO:19:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 37 amino acids(B) TYPE: amino acid (D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:19:PheIleThrSerGluProThrGlnProProProThrSerProProLeu151015ValHisAspSerAspL euGluHisValLeuThrProSerThrSerTrp202530ThrThrLysIleLeu35(2) INFORMATION FOR SEQ ID NO:20:(i) SEQUENCE CHARACTERISTICS:(A) LENGTH: 11 amino acids (B) TYPE: amino acid(D) TOPOLOGY: linear(ii) MOLECULE TYPE: peptide(xi) SEQUENCE DESCRIPTION: SEQ ID NO:20:ProTyrValGluProThrThrThrGlnCysPhe1510

Number	Name	Date
4525300	Yoshida et al.	Jun 1985
4629783	Cosand	Dec 1986
5066579	Reyes	Nov 1991

Peptides and analogues and mixtures thereof for detecting antibodies to HTLV-I and HTLV-II viruses

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Entry
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